mRNA_Ecto-sp13_S_contig14897.3725.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig14897.3725.1
Unique NamemRNA_Ecto-sp13_S_contig14897.3725.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig14897.3725.1 vs. uniprot
Match: D7FVZ4_ECTSI (Ferredoxin nitrite reductase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FVZ4_ECTSI)

HSP 1 Score: 239 bits (611), Expect = 8.640e-74
Identity = 116/117 (99.15%), Postives = 116/117 (99.15%), Query Frame = 1 
Query:    1 IKSGVVSCTGSQYCGLGLVETKNRAVDYAGKLDAMLDFPEGTKPPRIHWTGCPNSCGQAQVGDIGLMGGPARVNKKAVEGVNVFLGGGIGETHGLGEIAMKGIPAAEESLLPVLRDL 351
            I SGVVSCTGSQYCGLGLVETKNRAVDYAGKLDAMLDFPEGTKPPRIHWTGCPNSCGQAQVGDIGLMGGPARVNKKAVEGVNVFLGGGIGETHGLGEIAMKGIPAAEESLLPVLRDL
Sbjct:  516 ITSGVVSCTGSQYCGLGLVETKNRAVDYAGKLDAMLDFPEGTKPPRIHWTGCPNSCGQAQVGDIGLMGGPARVNKKAVEGVNVFLGGGIGETHGLGEIAMKGIPAAEESLLPVLRDL 632
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BLAST of mRNA_Ecto-sp13_S_contig14897.3725.1 vs. uniprot
Match: A0A5J4XDN5_9CHLO (Ferredoxin--nitrite reductase (Fragment) n=1 Tax=Trebouxia sp. A1-2 TaxID=2608996 RepID=A0A5J4XDN5_9CHLO)

HSP 1 Score: 145 bits (367), Expect = 1.120e-41
Identity = 71/117 (60.68%), Postives = 86/117 (73.50%), Query Frame = 1 
Query:    1 IKSGVVSCTGSQYCGLGLVETKNRAVDYAGKLDAMLDFPEGTKPPRIHWTGCPNSCGQAQVGDIGLMGGPARVNKKAVEGVNVFLGGGIGETHGLGEIAMKGIPAAEESLLPVLRDL 351
            +  G+VSCTG+Q+CG+ ++ETKNRA+D   KL+  LD PE     RIHWTGCPNSCGQAQVGDIGLMGGPA+++ KAVEGV +F GG IGE   L     KG+P  E  LLP LR+L
Sbjct:   93 LSRGLVSCTGAQFCGVAIIETKNRAMDIVEKLEQQLDIPEAV---RIHWTGCPNSCGQAQVGDIGLMGGPAKLDGKAVEGVRLFRGGIIGENPTLATEFEKGVPCEESILLPKLREL 206
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BLAST of mRNA_Ecto-sp13_S_contig14897.3725.1 vs. uniprot
Match: A0A5J4XGH9_9CHLO (Assimilatory nitrite reductase (Ferredoxin) n=1 Tax=Trebouxia sp. A1-2 TaxID=2608996 RepID=A0A5J4XGH9_9CHLO)

HSP 1 Score: 145 bits (367), Expect = 3.680e-40
Identity = 71/117 (60.68%), Postives = 86/117 (73.50%), Query Frame = 1 
Query:    1 IKSGVVSCTGSQYCGLGLVETKNRAVDYAGKLDAMLDFPEGTKPPRIHWTGCPNSCGQAQVGDIGLMGGPARVNKKAVEGVNVFLGGGIGETHGLGEIAMKGIPAAEESLLPVLRDL 351
            +  G+VSCTG+Q+CG+ ++ETKNRA+D   KL+  LD PE     RIHWTGCPNSCGQAQVGDIGLMGGPA+++ KAVEGV +F GG IGE   L     KG+P  E  LLP LR+L
Sbjct:  228 LSRGLVSCTGAQFCGVAIIETKNRAMDIVEKLEQQLDIPEAV---RIHWTGCPNSCGQAQVGDIGLMGGPAKLDGKAVEGVRLFRGGIIGENPTLATEFEKGVPCEESILLPKLREL 341
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BLAST of mRNA_Ecto-sp13_S_contig14897.3725.1 vs. uniprot
Match: A0A7S2X967_9EUKA (Hypothetical protein n=1 Tax=Lotharella oceanica TaxID=641309 RepID=A0A7S2X967_9EUKA)

HSP 1 Score: 142 bits (358), Expect = 8.980e-40
Identity = 70/125 (56.00%), Postives = 88/125 (70.40%), Query Frame = 1 
Query:    1 IKSGVVSCTGSQYCGLGLVETKNRAVDYAGKLDAMLDFPEGTKPPRIHWTGCPNSCGQAQVGDIGLMGGPARVNKK--------AVEGVNVFLGGGIGETHGLGEIAMKGIPAAEESLLPVLRDL 351
            +  G+VSCTG+Q+CG+ L+ETKNRAV    KL+  L  P    P RIHWTGCPNSCGQAQ+ D+GLMG PAR+ K+        AVEGVN+FLGG +GE   LGE+  KGIPA  E L+PV+ ++
Sbjct:  136 LSRGLVSCTGAQFCGIALIETKNRAVALTKKLEEKLKLPA---PVRIHWTGCPNSCGQAQIADVGLMGAPARIEKEVDGKIKKVAVEGVNIFLGGKVGEDPFLGEVYKKGIPADFEYLMPVMEEI 257
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BLAST of mRNA_Ecto-sp13_S_contig14897.3725.1 vs. uniprot
Match: A0A7S0E024_9CRYP (Hypothetical protein n=2 Tax=Cryptophyceae TaxID=3027 RepID=A0A7S0E024_9CRYP)

HSP 1 Score: 144 bits (362), Expect = 2.540e-39
Identity = 71/125 (56.80%), Postives = 91/125 (72.80%), Query Frame = 1 
Query:    1 IKSGVVSCTGSQYCGLGLVETKNRAVDYAGKLDAMLDFPEGTKPPRIHWTGCPNSCGQAQVGDIGLMGGPARVNKK--------AVEGVNVFLGGGIGETHGLGEIAMKGIPAAEESLLPVLRDL 351
            +  G+VSCTGSQ+CG+ L+ETKNRA++ + +L+  L       P RIHWTGCPNSCGQAQV DIGLMGGPARV K+        AVEGVN+FLGG +GE   LGE+  KG+PA  + L+PV++D+
Sbjct:  243 LSRGLVSCTGSQFCGVALIETKNRAIELSKRLEEELKVD---MPVRIHWTGCPNSCGQAQVADIGLMGGPARVEKEIDGKVKKVAVEGVNIFLGGKVGEDPFLGEVYKKGVPADYKYLIPVMKDI 364
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BLAST of mRNA_Ecto-sp13_S_contig14897.3725.1 vs. uniprot
Match: A0A7S2QSF0_9EUKA (Hypothetical protein n=1 Tax=Norrisiella sphaerica TaxID=552664 RepID=A0A7S2QSF0_9EUKA)

HSP 1 Score: 145 bits (367), Expect = 2.210e-38
Identity = 74/125 (59.20%), Postives = 89/125 (71.20%), Query Frame = 1 
Query:    1 IKSGVVSCTGSQYCGLGLVETKNRAVDYAGKLDAMLDFPEGTKPPRIHWTGCPNSCGQAQVGDIGLMGGPARVNKK--------AVEGVNVFLGGGIGETHGLGEIAMKGIPAAEESLLPVLRDL 351
            +  G+VSCTG+Q+CG+ L+ETKNRAV    KL+  L      +P RIHWTGCPNSCGQAQV DIGLMG PARV K+        AVEGVN+FLGG +GE   LGE+  KGIPA EE LLPV+ ++
Sbjct:  565 LSRGLVSCTGAQFCGIALIETKNRAVALTRKLEQRLSLD---RPVRIHWTGCPNSCGQAQVADIGLMGAPARVEKEVDGKLKKVAVEGVNIFLGGKVGEDPFLGEVFKKGIPADEEYLLPVMEEI 686
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BLAST of mRNA_Ecto-sp13_S_contig14897.3725.1 vs. uniprot
Match: A0A7S0D8G4_9EUKA (Hypothetical protein n=1 Tax=Amorphochlora amoebiformis TaxID=1561963 RepID=A0A7S0D8G4_9EUKA)

HSP 1 Score: 143 bits (360), Expect = 1.020e-37
Identity = 69/122 (56.56%), Postives = 91/122 (74.59%), Query Frame = 1 
Query:   10 GVVSCTGSQYCGLGLVETKNRAVDYAGKLDAMLDFPEGTKPPRIHWTGCPNSCGQAQVGDIGLMGGPARVNKK--------AVEGVNVFLGGGIGETHGLGEIAMKGIPAAEESLLPVLRDL 351
            G+VSCTG+Q+CG+ L+ETKNRA++ + +L+  L  P   +P RIHWTGCPNSCGQAQ+ DIGLMG PARV K+        AVEGVN+FLGG +GE   LG+I  KG+PA  +SL+PV+ ++
Sbjct:  458 GLVSCTGAQFCGIALIETKNRALELSKRLEEKLKLP---RPVRIHWTGCPNSCGQAQIADIGLMGAPARVEKEVNGKIKKVAVEGVNIFLGGRVGEDPFLGDIHKKGVPADFDSLIPVMTEI 576
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BLAST of mRNA_Ecto-sp13_S_contig14897.3725.1 vs. uniprot
Match: Q7XYQ0_BIGNA (Ferredoxin nitrite reductase n=1 Tax=Bigelowiella natans TaxID=227086 RepID=Q7XYQ0_BIGNA)

HSP 1 Score: 143 bits (361), Expect = 1.160e-37
Identity = 70/125 (56.00%), Postives = 91/125 (72.80%), Query Frame = 1 
Query:    1 IKSGVVSCTGSQYCGLGLVETKNRAVDYAGKLDAMLDFPEGTKPPRIHWTGCPNSCGQAQVGDIGLMGGPARVNKK--------AVEGVNVFLGGGIGETHGLGEIAMKGIPAAEESLLPVLRDL 351
            +  G+VSCTGSQ+CG+ L+ETKNRA++ + +L+  L       P RIHWTGCPNSCGQAQV DIGLMGGPARV K+        AVEGVN+FLGG +GE   LGE+  KG+PA  + L+P+++D+
Sbjct:  516 LSRGLVSCTGSQFCGVALIETKNRAIELSKRLEEELKVD---MPVRIHWTGCPNSCGQAQVADIGLMGGPARVEKEIDGKVKKVAVEGVNIFLGGKVGEDPFLGEVYKKGVPADYKYLIPIMKDI 637
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BLAST of mRNA_Ecto-sp13_S_contig14897.3725.1 vs. uniprot
Match: A0A383W6Q2_TETOB (Uncharacterized protein n=2 Tax=Scenedesmaceae TaxID=3086 RepID=A0A383W6Q2_TETOB)

HSP 1 Score: 142 bits (359), Expect = 1.250e-37
Identity = 70/117 (59.83%), Postives = 85/117 (72.65%), Query Frame = 1 
Query:    1 IKSGVVSCTGSQYCGLGLVETKNRAVDYAGKLDAMLDFPEGTKPPRIHWTGCPNSCGQAQVGDIGLMGGPARVNKKAVEGVNVFLGGGIGETHGLGEIAMKGIPAAEESLLPVLRDL 351
            ++ G+VSCTGSQ+CG GL+ETK RA+    KL+A L  P   +P RIHWTGCPNSCGQAQV DIGLMGGPA+++ KAVEGV +FLGG IGE   L      G+P  E+ LLP L+ L
Sbjct:  446 LERGLVSCTGSQFCGFGLIETKQRAMAVVSKLEASLHLP---RPVRIHWTGCPNSCGQAQVADIGLMGGPAKLDGKAVEGVKIFLGGKIGENPELAAEIEAGVPCHEDYLLPKLQQL 559
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BLAST of mRNA_Ecto-sp13_S_contig14897.3725.1 vs. uniprot
Match: A0A2P6TEN2_CHLSO (Ferredoxin--nitrite reductase n=1 Tax=Chlorella sorokiniana TaxID=3076 RepID=A0A2P6TEN2_CHLSO)

HSP 1 Score: 143 bits (360), Expect = 1.380e-37
Identity = 72/114 (63.16%), Postives = 86/114 (75.44%), Query Frame = 1 
Query:   10 GVVSCTGSQYCGLGLVETKNRAVDYAGKLDAMLDFPEGTKPPRIHWTGCPNSCGQAQVGDIGLMGGPARVNKKAVEGVNVFLGGGIGETHGLGEIAMKGIPAAEESLLPVLRDL 351
            G+VSCTG+Q+C L LVETKNRA++   KL+A LD P   +  R+HWTGCPNSCGQAQVGDIGLMG PA+++ KAVEGV +FLGG IGE   L +   +GIP  E  LLP LRDL
Sbjct:  478 GLVSCTGAQFCSLALVETKNRALEVVRKLEAELDIP---RLVRMHWTGCPNSCGQAQVGDIGLMGAPAKLDGKAVEGVKIFLGGKIGENPELAKEFERGIPVDESILLPRLRDL 588
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig14897.3725.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FVZ4_ECTSI8.640e-7499.15Ferredoxin nitrite reductase n=2 Tax=Ectocarpus Ta... [more]
A0A5J4XDN5_9CHLO1.120e-4160.68Ferredoxin--nitrite reductase (Fragment) n=1 Tax=T... [more]
A0A5J4XGH9_9CHLO3.680e-4060.68Assimilatory nitrite reductase (Ferredoxin) n=1 Ta... [more]
A0A7S2X967_9EUKA8.980e-4056.00Hypothetical protein n=1 Tax=Lotharella oceanica T... [more]
A0A7S0E024_9CRYP2.540e-3956.80Hypothetical protein n=2 Tax=Cryptophyceae TaxID=3... [more]
A0A7S2QSF0_9EUKA2.210e-3859.20Hypothetical protein n=1 Tax=Norrisiella sphaerica... [more]
A0A7S0D8G4_9EUKA1.020e-3756.56Hypothetical protein n=1 Tax=Amorphochlora amoebif... [more]
Q7XYQ0_BIGNA1.160e-3756.00Ferredoxin nitrite reductase n=1 Tax=Bigelowiella ... [more]
A0A383W6Q2_TETOB1.250e-3759.83Uncharacterized protein n=2 Tax=Scenedesmaceae Tax... [more]
A0A2P6TEN2_CHLSO1.380e-3763.16Ferredoxin--nitrite reductase n=1 Tax=Chlorella so... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig14897contigEcto-sp13_S_contig14897:14..1063 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score239.2
Seed ortholog evalue9.7e-61
Seed eggNOG ortholog2880.D7FVZ4
Preferred nameNIR
Model size351
KEGG rclassRC00176
KEGG koko:K00366
KEGG ReactionR00790
KEGG Pathwayko00910,ko01120,map00910,map01120
KEGG ModuleM00531
Hectar predicted targeting categoryother localisation
GOsGO:0001101,GO:0003674,GO:0003824,GO:0005575,GO:0005576,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0008150,GO:0008152,GO:0009507,GO:0009532,GO:0009536,GO:0009570,GO:0010035,GO:0010167,GO:0016020,GO:0016491,GO:0016661,GO:0016662,GO:0042221,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0048046,GO:0050421,GO:0050896,GO:0055114,GO:0098809,GO:1901698,GO:1901700
Exons2
EggNOG free text desc.sulfite reductase (ferredoxin) activity
EggNOG OGsCOG0155@1,KOG0560@2759
EC1.7.7.1
Cds size351
COG Functional cat.P
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462794.4920433-CDS-Ecto-sp13_S_contig14897:13..1841681462794.4920433-CDS-Ecto-sp13_S_contig14897:13..184Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig14897 14..184 -
1681462794.50411-CDS-Ecto-sp13_S_contig14897:883..10631681462794.50411-CDS-Ecto-sp13_S_contig14897:883..1063Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig14897 884..1063 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig14897.3725.1prot_Ecto-sp13_S_contig14897.3725.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig14897 14..1063 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig14897.3725.1

>prot_Ecto-sp13_S_contig14897.3725.1 ID=prot_Ecto-sp13_S_contig14897.3725.1|Name=mRNA_Ecto-sp13_S_contig14897.3725.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=117bp
IKSGVVSCTGSQYCGLGLVETKNRAVDYAGKLDAMLDFPEGTKPPRIHWT
GCPNSCGQAQVGDIGLMGGPARVNKKAVEGVNVFLGGGIGETHGLGEIAM
KGIPAAEESLLPVLRDL
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mRNA from alignment at Ecto-sp13_S_contig14897:14..1063-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig14897.3725.1 ID=mRNA_Ecto-sp13_S_contig14897.3725.1|Name=mRNA_Ecto-sp13_S_contig14897.3725.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=1050bp|location=Sequence derived from alignment at Ecto-sp13_S_contig14897:14..1063- (Ectocarpus species13 EcNAP12_S_4_19m)
ATCAAGTCTGGCGTGGTGTCATGCACGGGGTCCCAGTACTGCGGCCTGGG
CCTGGTAGAAACCAAGAACAGGGCGGTGGACTATGCGGGCAAACTAGATG
CGATGCTGGACTTCCCCGAGGGAACCAAGCCGCCGCGTATCCACTGGACG
GGGTGTCCCAACAGCTGTGGCCAGGCGCAGGTATGACGTAGGGGTAGGGA
GGGGGGGGGGGGGATCCGTGGATGAAGGAGAATTGATTATACTTGTACGA
GGACGCCGACTTTGTTCACGCGCCCAATTAGGGAGGAAGGGGACAGATAC
GTTTCATTTTTTGTGTGTGTTCAAGCAGACAACACACGATGTTCAAGCAG
ACAAGACGATGGTGTTGTAACGCAAATCCGACCGGCCAGATCATTTAAAG
AGAATTTTCGTCTGTTCAGGACAACCTTTTTTCAACGTTCTTCTCCACAG
GTTGATGTTTGAAACAGCAACGCGTTCATGATGACGGCATCGAGCTTCGC
CAATACCTGCTTTGTGTTCGTGGCCGTGGTGGTGTGTGGTGTGTGCCTCA
GTGCACGCTCGTCCGCGGGCCTTTTCCGTCCTGAGTGGTGGACCCCACAC
CTGCCCGCAACTGCCTTGTACTTGACGAACACAGAAACTTGCGTGACCCC
TCCCCCCCATAGCCAGCTGAACAAACTGCAGTGCGGCCGCCTGAACAGTT
TACTTGCCTTCGAGTCAACGCATAGTGTAGCACCAGATCAAGCGAAGACG
TGCTGTTACCCCCCCAAAATCAGACCTTTCTCCAAAACAAAAGGTCAGGC
ATTTGTTGGTACCACAACTTTAACTCCGAGATCACGTTTGTGTGCGTGTG
CGTGTACGCTTTCGCCTCTCTCTTGGAAGGTGGGCGACATCGGCCTGATG
GGTGGGCCGGCTAGGGTGAACAAGAAGGCCGTCGAGGGCGTGAACGTGTT
CTTGGGGGGAGGCATCGGCGAGACTCACGGGCTGGGAGAGATCGCGATGA
AGGGCATCCCCGCGGCGGAGGAGTCTCTGCTCCCGGTGCTTCGCGACCTC
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig14897:14..1063-

>mRNA_Ecto-sp13_S_contig14897.3725.1 ID=mRNA_Ecto-sp13_S_contig14897.3725.1|Name=mRNA_Ecto-sp13_S_contig14897.3725.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=351bp|location=Sequence derived from alignment at Ecto-sp13_S_contig14897:14..1063- (Ectocarpus species13 EcNAP12_S_4_19m)
ATCAAGTCTGGCGTGGTGTCATGCACGGGGTCCCAGTACTGCGGCCTGGG
CCTGGTAGAAACCAAGAACAGGGCGGTGGACTATGCGGGCAAACTAGATG
CGATGCTGGACTTCCCCGAGGGAACCAAGCCGCCGCGTATCCACTGGACG
GGGTGTCCCAACAGCTGTGGCCAGGCGCAGGTGGGCGACATCGGCCTGAT
GGGTGGGCCGGCTAGGGTGAACAAGAAGGCCGTCGAGGGCGTGAACGTGT
TCTTGGGGGGAGGCATCGGCGAGACTCACGGGCTGGGAGAGATCGCGATG
AAGGGCATCCCCGCGGCGGAGGAGTCTCTGCTCCCGGTGCTTCGCGACCT
C
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