mRNA_Ecto-sp13_S_contig146.3533.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig146.3533.1
Unique NamemRNA_Ecto-sp13_S_contig146.3533.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig146.3533.1 vs. uniprot
Match: A0A6H5K070_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K070_9PHAE)

HSP 1 Score: 494 bits (1273), Expect = 2.460e-170
Identity = 301/391 (76.98%), Postives = 312/391 (79.80%), Query Frame = 3 
Query:  132 LEWGRRYQEEELLSLRLEIGEVSREVEAETKQLRDREEIIRAIRDEQRQDRIKIQTLLALSQPITPDITVVFQALAPDACPSSLPPPSRAARPLDRSFVSQNNARAVASNSPSRRSVAAAAAATAGTNAGRGVSGEEDRRGVFGDESPVERPRHVERRRIRQQEQRPRRRRYLNDGIGSDGGSGGEQRNPEGERDNXXXXXXXXXXXXAGSDSHTHQEETTGGVSYGLRPGRRWTSERGEGNGGHADRRRGGRGGGAPG-RXXXRPRSGDGGPSLGTRKSREANKLRCTRGYQQHLERRLELTQRHLRELERLEGSYHETLTRDKQEREIHARIQKDNAARVVEALGRRTAEVEKRLSGATDDYLRLRHKAKEAHAVSAEEQRQCSEAREK 1301
            L+WGRRYQEEELL+LRLE+GEVS EVEAE KQLRDREEIIRAIRDEQRQDRIKIQTLLALSQPITPDITVVFQALAPDACP  LPPPSR ARPLDRS+V QNN RAVASNSPSRRSVAAAAA TAG NAGRG                               EQRPRRRRYLNDGIGSDG SGGEQ N EGER+                 +   +EE TGGVSYGL PGRRWTSER  GNGGHADRRRGGR GG PG R   RPRSGDGG SLGTRKSREAN+L CTRGYQQHLERRLEL +RHLRELERLEGSYHETLTRDKQEREIHAR+Q+DNAARVVEAL RR AEVEKRLSGATDDYLRLRHKAKEAHAVSAEEQRQC EAREK
Sbjct:   13 LQWGRRYQEEELLALRLEVGEVSGEVEAEIKQLRDREEIIRAIRDEQRQDRIKIQTLLALSQPITPDITVVFQALAPDACPPPLPPPSRGARPLDRSYVPQNNRRAVASNSPSRRSVAAAAA-TAGRNAGRG-------------------------------EQRPRRRRYLNDGIGSDGCSGGEQINMEGERN-----------------AGRGEEEATGGVSYGLYPGRRWTSERVGGNGGHADRRRGGREGGVPGSRRRRRPRSGDGGGSLGTRKSREANRL-CTRGYQQHLERRLELNERHLRELERLEGSYHETLTRDKQEREIHARVQRDNAARVVEALERRNAEVEKRLSGATDDYLRLRHKAKEAHAVSAEEQRQCREAREK 353
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BLAST of mRNA_Ecto-sp13_S_contig146.3533.1 vs. uniprot
Match: D8LLH3_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LLH3_ECTSI)

HSP 1 Score: 347 bits (889), Expect = 1.070e-113
Identity = 231/424 (54.48%), Postives = 240/424 (56.60%), Query Frame = 3 
Query:   30 MECYQEVMRAIERERINFLDKIDLVQPSFEEQHQLEWGRRYQEEELLSLRLEIGEVSREVEAETKQLRDREEIIRAIRDEQRQDRIKIQTLLALSQPITPDITVVFQALAPDACPSSLPPPSRAARPLDRSFVSQNNARAVASNSPSRRSVAAAAAATAGTNAGRGVSGEEDRRGVFGDESPVERPRHVERRRIRQQEQRPRRRRYLNDGIGSDGGSGGEQRNPEGERDNXXXXXXXXXXXXAGSDSHTHQEETTGGVSYGLRPGRRWTSERGEGNGGHADRRRGGRGGGAPGRXXXRPRSGDGGPSLGTRKSREANKLRCTRGYQQHLERRLELTQRHLRELERLEGSYHETLTRDKQEREIHARIQKDNAARVVEALGRRTAEVEKRLSGATDDYLRLRHKAKEAHAVSAEEQRQCSEAREK 1301
            MECYQE MRAIERERIN L+KIDLVQPSFEEQHQLEWGRRYQEEELL+LRLE+GEVSREVEAETKQLRDREEIIRAIRDEQRQDRIKIQTLLALSQPITPDITVVFQALAPDACP  LPPPSR +RPLDRS V QNN RAV SNSPSRRSVAAAA  TAGT+AGRG                                                                                                                                                                           RHLRELERLEGS+HETLTRDKQERE+HAR+QKDNAARV+EALGRRTAEVEKRLSGATDDYLRLRHKAKEAHAVSAE QRQC EAREK
Sbjct:    1 MECYQEAMRAIERERINVLNKIDLVQPSFEEQHQLEWGRRYQEEELLALRLEVGEVSREVEAETKQLRDREEIIRAIRDEQRQDRIKIQTLLALSQPITPDITVVFQALAPDACPPPLPPPSRGSRPLDRSSVPQNNRRAVTSNSPSRRSVAAAA--TAGTSAGRG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------RHLRELERLEGSFHETLTRDKQEREMHARVQKDNAARVMEALGRRTAEVEKRLSGATDDYLRLRHKAKEAHAVSAEGQRQCGEAREK 251
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BLAST of mRNA_Ecto-sp13_S_contig146.3533.1 vs. uniprot
Match: A0A835ZIT2_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZIT2_9STRA)

HSP 1 Score: 56.6 bits (135), Expect = 4.880e-5
Identity = 40/110 (36.36%), Postives = 56/110 (50.91%), Query Frame = 3 
Query:   81 FLDKIDLVQPSFEEQHQLEWGRRYQEEELL-------SLRLEIGEVSREVEAETKQLRDREEIIRAIRDEQRQDRIKIQTLLALSQPITPDITVVFQALAPDACPSSLPP 389
            F  +I++VQPS EE+H++EW     E EL        SLR   G     + A       R   I  +  EQR+DR ++Q LLAL QP++ D+TV+F        P + PP
Sbjct:   46 FRKQIEMVQPSQEERHRIEWQLEALERELAVASGEAASLRASTGTAGAAIAA-------RAAAIAELLAEQREDRTRVQRLLALCQPVSHDVTVLFDHFGASEGPRAAPP 148
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig146.3533.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 3
Match NameE-valueIdentityDescription
A0A6H5K070_9PHAE2.460e-17076.98Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LLH3_ECTSI1.070e-11354.48Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A835ZIT2_9STRA4.880e-536.36Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig146contigEcto-sp13_S_contig146:32122..36120 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Model size1301
Hectar predicted targeting categoryother localisation
Exons5
Ec32 ortholog descriptionhypothetical protein
Ec32 orthologEc-00_004610.1
Cds size1272
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig146.3533.1prot_Ecto-sp13_S_contig146.3533.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig146 32151..36120 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462779.959876-UTR-Ecto-sp13_S_contig146:32121..321501681462779.959876-UTR-Ecto-sp13_S_contig146:32121..32150Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig146 32122..32150 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462779.9683113-CDS-Ecto-sp13_S_contig146:32150..322581681462779.9683113-CDS-Ecto-sp13_S_contig146:32150..32258Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig146 32151..32258 +
1681462779.9762034-CDS-Ecto-sp13_S_contig146:32991..331321681462779.9762034-CDS-Ecto-sp13_S_contig146:32991..33132Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig146 32992..33132 +
1681462779.9840546-CDS-Ecto-sp13_S_contig146:33494..342561681462779.9840546-CDS-Ecto-sp13_S_contig146:33494..34256Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig146 33495..34256 +
1681462779.992041-CDS-Ecto-sp13_S_contig146:35169..353031681462779.992041-CDS-Ecto-sp13_S_contig146:35169..35303Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig146 35170..35303 +
1681462779.9998796-CDS-Ecto-sp13_S_contig146:35993..361201681462779.9998796-CDS-Ecto-sp13_S_contig146:35993..36120Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig146 35994..36120 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig146.3533.1

>prot_Ecto-sp13_S_contig146.3533.1 ID=prot_Ecto-sp13_S_contig146.3533.1|Name=mRNA_Ecto-sp13_S_contig146.3533.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=424bp
MECYQEVMRAIERERINFLDKIDLVQPSFEEQHQLEWGRRYQEEELLSLR
LEIGEVSREVEAETKQLRDREEIIRAIRDEQRQDRIKIQTLLALSQPITP
DITVVFQALAPDACPSSLPPPSRAARPLDRSFVSQNNARAVASNSPSRRS
VAAAAAATAGTNAGRGVSGEEDRRGVFGDESPVERPRHVERRRIRQQEQR
PRRRRYLNDGIGSDGGSGGEQRNPEGERDNDEECEEEEGGDDAGSDSHTH
QEETTGGVSYGLRPGRRWTSERGEGNGGHADRRRGGRGGGAPGRRRRRPR
SGDGGPSLGTRKSREANKLRCTRGYQQHLERRLELTQRHLRELERLEGSY
HETLTRDKQEREIHARIQKDNAARVVEALGRRTAEVEKRLSGATDDYLRL
RHKAKEAHAVSAEEQRQCSEAREK
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mRNA from alignment at Ecto-sp13_S_contig146:32122..36120+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig146.3533.1 ID=mRNA_Ecto-sp13_S_contig146.3533.1|Name=mRNA_Ecto-sp13_S_contig146.3533.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=3999bp|location=Sequence derived from alignment at Ecto-sp13_S_contig146:32122..36120+ (Ectocarpus species13 EcNAP12_S_4_19m)
CCTCAAGGCCGTCCACGACAGCCAGGACGATGGAGTGCTACCAGGAGGTG
ATGCGCGCGATAGAGAGGGAGCGCATAAACTTTTTGGACAAGATCGACCT
GGTTCAGCCTTCCTTCGAGGAGCAGCATCAACTCGAGGTGGGCAGTAATC
AAACGTAAAATACCCAACGGTTTCCACTATCTACAGCAGTGTAATTGTTT
TCTCTCGACAGCACAGATACTTCATGGCGGCATGATGCTCCCGGGCGCTA
GACTCTCTCACGTGTCAGCACAACGAGAGCTACGTGGGCGTGTCACCTGA
GCACAACTTTTAGCGGTGATGTTCGCTGTTCAGAACACATAAAGTGTGTG
TGTGTGCGGTCTGTTTGGTCTCCGAGAATGAAGTCAAGCACACAGCGATC
ACGCGCTAAGAAAGAAGCCTCGCCAACGTTGTTCCCAGTGCGTGCGGCTG
TGTTTCTTGGGCCATGTCGCGAGTGCCACATTCGAAGCAGTCCACGAAAG
CATTGGGTGTTGGAACGTTGGCTTTCCTTCCGATCTAGCAAATAGCCCAC
AGATCTTGGCGTAGCGATATGGTAAAGCTTACCAACTGGAGTGGACGACG
AACTCCGCACACATGCTACACTCCTCACAGTAAACATCACCCATTGTTGC
TTATAGAAGGACCTTCGGAAGTGTGCGAAGCGGGCGCGTTCTGCTCTCTA
GACCAGGCAGTACCTGTAGCTGCCGTAGAGGATCGTCGGAGATCAAGGCA
GATTGGGGAGGGGGTACGGCTGCCAGGTTCAAAGCACTGCAAAGCAGCAT
CTGCCGCGAACGCTGCTTTGTGCACCTGATGACCATCCTTGCTTCAACTC
TGTTAAACTGTTGTCTGCAGTGGGGGCGCCGTTACCAGGAGGAAGAGCTT
CTATCCCTGCGGTTGGAGATCGGTGAGGTCTCGAGAGAGGTGGAAGCGGA
GACAAAGCAGCTTCGAGACAGGGAGGAGATCATCCGGGCCATCCGTGACG
AGCAACGACAGGTACGGCCCTATGTGGGGATAGGTTCTACCCGTGCGGTG
AGGCCGTGGAGAGACTCGTCCCCACCCCCCGCCCCTCGGCGTCGGTCAAT
GAGGCACCGCTTTTCGCCATTATGAGGAAAAGCGGCAGTTTGGCCGGGTG
GCGTCTGAGTGCTCTGGTCGTGGTGGCTATTCGTCACTACTGTTGATGTT
GGTTTGCTCGACTTTATCTTGTGTACCAATTTGTAGTGCAGCAAGCCCCT
GCCTCAAGTCGGACCCGAATACATACACGTGCCCCAAAATGCAACAATAC
TGCTGTACGCCCAGTTTTCTCTTTCCGTGTTCGCTGTGCAATCACCACCG
CCCCGCCCCGCCAACCCGTGCAGGACCGGATAAAAATCCAGACGTTACTC
GCCCTGTCTCAGCCCATCACGCCGGACATCACCGTGGTATTCCAGGCTTT
GGCGCCCGACGCCTGCCCCTCGTCCTTGCCACCGCCGTCGAGAGCTGCTC
GACCCCTCGATAGGTCGTTTGTCTCTCAAAACAATGCAAGAGCGGTAGCT
TCGAACTCGCCTTCACGCAGGAGTGTTGCGGCGGCGGCGGCGGCGACAGC
CGGGACGAATGCTGGGCGAGGTGTAAGTGGAGAGGAAGATAGAAGAGGCG
TTTTTGGAGACGAAAGTCCTGTCGAACGCCCAAGACACGTTGAACGTCGT
AGAATCAGGCAGCAGGAGCAGCGGCCGCGGCGACGGCGGTACTTGAACGA
CGGTATCGGCAGCGACGGGGGCAGCGGTGGGGAACAAAGAAATCCGGAGG
GAGAAAGGGATAACGACGAAGAGTGCGAGGAGGAAGAAGGTGGCGATGAT
GCCGGCAGTGATAGCCACACACATCAAGAGGAGACGACTGGAGGGGTGAG
CTACGGGTTGCGTCCGGGACGGCGATGGACCTCGGAGAGAGGGGAGGGAA
ATGGAGGCCACGCAGACAGAAGAAGGGGGGGGAGGGGGGGAGGAGCTCCT
GGCAGGAGGAGGAGGAGGCCGAGAAGTGGCGACGGTGGGCCCAGTCTAGG
CACGAGAAAAAGCAGGGAGGCGAACAAGCTGAGGTGTACCCGGGGATATC
AACAGCATCTGGAACGAAGATTGGAACTCACCCAGGTTCGTCGGCAGAAT
ACGTTGCCTCCCCCCCCCCAGAACCCACAATGAGTGTACGGTGAAAAAGA
CACAAATCGCCCTTGATGGACATTTGGGAATGTAAGTTCTTTTGAACAAA
TCACTTGTAAATGTTTCTACACGACTTTGTGCTACATCGGGGGTCACAGT
ATTTCGGGCTACGGTGCATACCACCGTCTCGCCACCTAGAAGAAGCTATC
TACCGCAGAGTCATCAGGAAAAAACAATCGTCCGCAAGCGCCTTCCTTGA
TCTTTCGAACAGTCTCCACAAGGTTGGAGGAAAATGTGTTGCCATCAGGA
GTGTGCGCTCCTCAACAATTGTGGAGGTGGCAGTGCGACTGCTCCACTTT
TCTAGAACTGGAGTAGTCACGGGTGCATTCTGCTAACGTAGCTCAACTAG
GGTGAATTTCTGGTGATGTCGTCCACTCTGTTTTAGCGGATCTGATGCGT
GCATTTTGGTGCTGAGAGCTACGAAGACGAGGTTTCAAGTATGTAGCAGG
GCTCACAAAGGTTATGACATGTCATTTGTCCACAATCAATCCCCTCTGGA
CACAGAACAGGACGGTTTTAGGCTTCCTGCTGATTTCATGACAAGCGCGG
TTTATCTGAGTTGCATCAGCGGTGACAAAAGCCTTGGTCGAATTAATCGT
CAGCGGCAACAGAGGGGATGAGGTGAAGGGAATGATTCCTACAAGAAGCA
AGAGCTTTCGACAACGCAGAAATCTGTTGTTTTTGAATTGTGCTCGGCTG
AAACACGCGTGGCACAGACAGTCTTCATCCCTGGGGCAGGGTCCGGGGGT
AAGCTCCGTGAGGCCCTCTTCTCCCAGTTGCCAGAACTGTTTTCGCCACC
TCATACGTCTTGCCTATGCTCCTTGCTCGACTGCGTTTTGATTCCTAGCG
GCACTTGCGGGAACTGGAGCGCCTCGAAGGTTCGTACCACGAAACGCTAA
CCAGGGATAAGCAGGAGAGAGAGATACATGCTCGGATTCAGAAGGACAAC
GCCGCCCGCGTTGTCGAAGCCCTGGGACGGAGGTGAGTCGAAGCTGTTAA
ACACAGTGGCGGCATGTGTCATCAGTCTTACACAATTCTTGTTGGCGTTG
AAGGCGTCTTGGTTGCGGAGCAATGGATTTTTCGCGCGCCCATGAGTTCG
GCCGCACGTTTTACATCGCAACCTTGTGAATCTCTAGATTGAGGGTTCGA
ATCCCTCCGGAGTGAAAATTCGAACAATAATTATTGTAGAGGTTTCGAGA
GCATGGTCGTGAGTAAACACGAAAACGAGGGAAGATCCAACTGTTGAACC
CTTCGCGTTCGAATTAAAGGCAAGTAAGGGGAACAGGCGTTGAAAAAAGA
ATAAAAAATACGGTGTTTAACTCCCTCCCATGCGCCCCGAACTACTGTGT
CAAACATAGCTAAATTCGGTATCGATCGTCGCCCAAAATAGATGAGACGA
AAACAATAACACAGCAACAATGTAATGATGGTTTGAGCTTGTTTTGTCGC
TTATTCAGGAAGCGTGTGAAATTTATTTCCGTGTGCACACCTGGTATTTA
ATTTTTGTGTGTATGTCTGTGATTGTTGCCTGTTTTTTCTCGCATCGAGC
ACGCTCATCCTGTAGGCTGATATTATGAAAAGACGTCGGTCTTGTCAGTG
CGGCTGAAAACACAAAGCGCTCCTGAAGGCCATCATCGGCTTTTTGTGTG
GGCACAATCGTGTTGGTTGCAGGACCGCCGAGGTGGAGAAGCGGTTATCC
GGGGCAACCGACGACTACCTTCGTTTGAGACACAAGGCAAAAGAGGCTCA
CGCCGTGTCTGCGGAGGAGCAAAGGCAGTGCAGCGAGGCGCGGGAAAAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig146:32122..36120+

>mRNA_Ecto-sp13_S_contig146.3533.1 ID=mRNA_Ecto-sp13_S_contig146.3533.1|Name=mRNA_Ecto-sp13_S_contig146.3533.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=1272bp|location=Sequence derived from alignment at Ecto-sp13_S_contig146:32122..36120+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGGAGTGCTACCAGGAGGTGATGCGCGCGATAGAGAGGGAGCGCATAAA
CTTTTTGGACAAGATCGACCTGGTTCAGCCTTCCTTCGAGGAGCAGCATC
AACTCGAGTGGGGGCGCCGTTACCAGGAGGAAGAGCTTCTATCCCTGCGG
TTGGAGATCGGTGAGGTCTCGAGAGAGGTGGAAGCGGAGACAAAGCAGCT
TCGAGACAGGGAGGAGATCATCCGGGCCATCCGTGACGAGCAACGACAGG
ACCGGATAAAAATCCAGACGTTACTCGCCCTGTCTCAGCCCATCACGCCG
GACATCACCGTGGTATTCCAGGCTTTGGCGCCCGACGCCTGCCCCTCGTC
CTTGCCACCGCCGTCGAGAGCTGCTCGACCCCTCGATAGGTCGTTTGTCT
CTCAAAACAATGCAAGAGCGGTAGCTTCGAACTCGCCTTCACGCAGGAGT
GTTGCGGCGGCGGCGGCGGCGACAGCCGGGACGAATGCTGGGCGAGGTGT
AAGTGGAGAGGAAGATAGAAGAGGCGTTTTTGGAGACGAAAGTCCTGTCG
AACGCCCAAGACACGTTGAACGTCGTAGAATCAGGCAGCAGGAGCAGCGG
CCGCGGCGACGGCGGTACTTGAACGACGGTATCGGCAGCGACGGGGGCAG
CGGTGGGGAACAAAGAAATCCGGAGGGAGAAAGGGATAACGACGAAGAGT
GCGAGGAGGAAGAAGGTGGCGATGATGCCGGCAGTGATAGCCACACACAT
CAAGAGGAGACGACTGGAGGGGTGAGCTACGGGTTGCGTCCGGGACGGCG
ATGGACCTCGGAGAGAGGGGAGGGAAATGGAGGCCACGCAGACAGAAGAA
GGGGGGGGAGGGGGGGAGGAGCTCCTGGCAGGAGGAGGAGGAGGCCGAGA
AGTGGCGACGGTGGGCCCAGTCTAGGCACGAGAAAAAGCAGGGAGGCGAA
CAAGCTGAGGTGTACCCGGGGATATCAACAGCATCTGGAACGAAGATTGG
AACTCACCCAGCGGCACTTGCGGGAACTGGAGCGCCTCGAAGGTTCGTAC
CACGAAACGCTAACCAGGGATAAGCAGGAGAGAGAGATACATGCTCGGAT
TCAGAAGGACAACGCCGCCCGCGTTGTCGAAGCCCTGGGACGGAGGACCG
CCGAGGTGGAGAAGCGGTTATCCGGGGCAACCGACGACTACCTTCGTTTG
AGACACAAGGCAAAAGAGGCTCACGCCGTGTCTGCGGAGGAGCAAAGGCA
GTGCAGCGAGGCGCGGGAAAAG
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