mRNA_Ecto-sp13_S_contig12217.1868.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig12217.1868.1
Unique NamemRNA_Ecto-sp13_S_contig12217.1868.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig12217.1868.1 vs. uniprot
Match: D7FLF7_ECTSI (Probable Spc3 homolog, subunit of Signal Peptidase Complex n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FLF7_ECTSI)

HSP 1 Score: 205 bits (522), Expect = 5.210e-67
Identity = 105/105 (100.00%), Postives = 105/105 (100.00%), Query Frame = 1 
Query:    1 MHTAWVRVNAVVFFGLTVLLVLSVLTALSTYLHQGFPDVRKLGVSKFRTFRKSEGTDRLLLNIDVDADLAPAFNWNVKQLFAFVVAEYETPTNPVNQASQSVRFA 315
            MHTAWVRVNAVVFFGLTVLLVLSVLTALSTYLHQGFPDVRKLGVSKFRTFRKSEGTDRLLLNIDVDADLAPAFNWNVKQLFAFVVAEYETPTNPVNQASQSVRFA
Sbjct:    1 MHTAWVRVNAVVFFGLTVLLVLSVLTALSTYLHQGFPDVRKLGVSKFRTFRKSEGTDRLLLNIDVDADLAPAFNWNVKQLFAFVVAEYETPTNPVNQASQSVRFA 105
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BLAST of mRNA_Ecto-sp13_S_contig12217.1868.1 vs. uniprot
Match: A0A6V1NT77_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1NT77_HETAK)

HSP 1 Score: 130 bits (328), Expect = 1.710e-36
Identity = 62/97 (63.92%), Postives = 78/97 (80.41%), Query Frame = 1 
Query:    1 MHTAWVRVNAVVFFGLTVLLVLSVLTALSTYLHQGFPDVRKLGVSKFRTFRKSEGTDRLLLNIDVDADLAPAFNWNVKQLFAFVVAEYETPTNPVNQ 291
            M+T WVRVNAVVFFGLTVLLVL+++ A STYLH+G P+++ LGV+  R+ +   G DR L++ D+DADL PAFNWN+KQLF FVVAEY T  NP+NQ
Sbjct:    4 MNTVWVRVNAVVFFGLTVLLVLAIICAFSTYLHKGEPNIKYLGVNTIRSLKSHGGVDRALISFDLDADLTPAFNWNIKQLFVFVVAEYATEKNPLNQ 100
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BLAST of mRNA_Ecto-sp13_S_contig12217.1868.1 vs. uniprot
Match: A0A835YJ42_9STRA (Signal peptidase 22kDa subunit n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YJ42_9STRA)

HSP 1 Score: 129 bits (323), Expect = 8.740e-36
Identity = 60/97 (61.86%), Postives = 79/97 (81.44%), Query Frame = 1 
Query:    1 MHTAWVRVNAVVFFGLTVLLVLSVLTALSTYLHQGFPDVRKLGVSKFRTFRKSEGTDRLLLNIDVDADLAPAFNWNVKQLFAFVVAEYETPTNPVNQ 291
            M+T WVR+NAVVFFGLTVLL L+++TA STYLH+G P V+ L ++K ++FR     DR ++N D++ADLAPAFNWN+KQ+F FVVAE+ T TNP+NQ
Sbjct:    1 MNTVWVRLNAVVFFGLTVLLGLALMTATSTYLHEGHPTVKTLRLNKIKSFRSQSKVDRAIINFDLEADLAPAFNWNIKQIFVFVVAEFATKTNPLNQ 97
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BLAST of mRNA_Ecto-sp13_S_contig12217.1868.1 vs. uniprot
Match: A0A7S2XZ74_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XZ74_9STRA)

HSP 1 Score: 121 bits (303), Expect = 9.090e-33
Identity = 57/97 (58.76%), Postives = 76/97 (78.35%), Query Frame = 1 
Query:    1 MHTAWVRVNAVVFFGLTVLLVLSVLTALSTYLHQGFPDVRKLGVSKFRTFRKSEGTDRLLLNIDVDADLAPAFNWNVKQLFAFVVAEYETPTNPVNQ 291
            M+T WVR+NAVVFFG TVLL L+ + + ST+LH+G P ++ LG++  R+ R  +G DR+L++ D+DADL+ AFNWN+KQLF FVVAEY T TNP NQ
Sbjct:    1 MNTIWVRLNAVVFFGFTVLLCLACMCSFSTFLHKGEPHIKYLGLNTIRSMRAQKGGDRVLISFDIDADLSGAFNWNIKQLFVFVVAEYATKTNPFNQ 97
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BLAST of mRNA_Ecto-sp13_S_contig12217.1868.1 vs. uniprot
Match: A0A7S2SN40_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2SN40_9STRA)

HSP 1 Score: 117 bits (292), Expect = 6.490e-31
Identity = 58/97 (59.79%), Postives = 72/97 (74.23%), Query Frame = 1 
Query:    1 MHTAWVRVNAVVFFGLTVLLVLSVLTALSTYLHQGFPDVRKLGVSKFRTFRKSEGTDRLLLNIDVDADLAPAFNWNVKQLFAFVVAEYETPTNPVNQ 291
            MHT WVR+NAVVFFGLTV++VL+VLT  ST  H+G+P V  L ++   T R     D  +L+ D+ ADL+PAFNWN+KQLF FVVAEY T TNP+NQ
Sbjct:    1 MHTVWVRLNAVVFFGLTVIVVLAVLTWFSTVTHRGYPTVDTLQLNSIGTLRNHGERDIAILSFDLHADLSPAFNWNIKQLFVFVVAEYRTKTNPLNQ 97
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BLAST of mRNA_Ecto-sp13_S_contig12217.1868.1 vs. uniprot
Match: A0A7S4J3F9_9STRA (Hypothetical protein n=1 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4J3F9_9STRA)

HSP 1 Score: 112 bits (280), Expect = 2.970e-29
Identity = 58/99 (58.59%), Postives = 71/99 (71.72%), Query Frame = 1 
Query:    1 MHTAWVRVNAVVFFGLTVLLVLSVLTALSTYLHQGF--PDVRKLGVSKFRTFRKSEGTDRLLLNIDVDADLAPAFNWNVKQLFAFVVAEYETPTNPVNQ 291
            MHT WVR+NAVVFFGLTVLL LS+L ALS   H     PDV++L +   R+ +   G DR LL+ D+D DL PAF+WN+KQLF +VVA YE+ TNP NQ
Sbjct:    1 MHTVWVRLNAVVFFGLTVLLGLSILAALSKSGHSNRYRPDVKRLELKTLRSLKSHGGVDRALLSFDIDVDLRPAFHWNIKQLFVYVVASYESDTNPNNQ 99
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BLAST of mRNA_Ecto-sp13_S_contig12217.1868.1 vs. uniprot
Match: A0A7R9UGA1_9STRA (Hypothetical protein n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9UGA1_9STRA)

HSP 1 Score: 112 bits (281), Expect = 3.040e-29
Identity = 60/98 (61.22%), Postives = 68/98 (69.39%), Query Frame = 1 
Query:    1 MHTAWVRVNAVVFFGLTVLLVLSVLTALSTYL-HQGFPDVRKLGVSKFRTFRKSEGTDRLLLNIDVDADLAPAFNWNVKQLFAFVVAEYETPTNPVNQ 291
            MHT WVR+NAVVFF  TVLL LS L A+ T L H G P V  L + + R+ RK    DR +L  D+DADL  AFNWNVKQLF FVVAEY T TNP+NQ
Sbjct:   18 MHTIWVRLNAVVFFSFTVLLALSTLAAVQTMLFHDGQPVVHTLRLRELRSLRKHANIDRAMLTFDIDADLRGAFNWNVKQLFVFVVAEYATQTNPLNQ 115
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BLAST of mRNA_Ecto-sp13_S_contig12217.1868.1 vs. uniprot
Match: A0A7S2XM83_9STRA (Hypothetical protein (Fragment) n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2XM83_9STRA)

HSP 1 Score: 109 bits (273), Expect = 4.080e-28
Identity = 58/99 (58.59%), Postives = 72/99 (72.73%), Query Frame = 1 
Query:    1 MHTAWVRVNAVVFFGLTVLLVLSVLTALSTYLH--QGFPDVRKLGVSKFRTFRKSEGTDRLLLNIDVDADLAPAFNWNVKQLFAFVVAEYETPTNPVNQ 291
            MHT WVR+NAVVFFGLTVLL LSVL A+S   H  +  P V KL +++ R+ +   G DR LL+ D+ AD+ PAF+WN+KQLF +VVA YET TNP NQ
Sbjct:    5 MHTVWVRLNAVVFFGLTVLLGLSVLAAISAIGHSTRHKPVVSKLSLNQLRSLKSHGGVDRALLSFDLHADMRPAFHWNIKQLFVYVVASYETDTNPNNQ 103
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BLAST of mRNA_Ecto-sp13_S_contig12217.1868.1 vs. uniprot
Match: B8CCG8_THAPS (Uncharacterized protein n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8CCG8_THAPS)

HSP 1 Score: 107 bits (267), Expect = 2.730e-27
Identity = 55/99 (55.56%), Postives = 71/99 (71.72%), Query Frame = 1 
Query:    1 MHTAWVRVNAVVFFGLTVLLVLSVLTALSTYLH--QGFPDVRKLGVSKFRTFRKSEGTDRLLLNIDVDADLAPAFNWNVKQLFAFVVAEYETPTNPVNQ 291
            MHT WVR+NAVVFFGLTVLL LS+L ALS   H  +  P + KL ++  R+ +   G DR LL+ D+ AD+ PAF+WN+KQ+F +VVA Y+T TNP NQ
Sbjct:    1 MHTVWVRLNAVVFFGLTVLLCLSILAALSKIGHTRRYQPKIHKLALNNLRSVKNHGGVDRALLSFDLHADMNPAFHWNIKQIFVYVVATYKTDTNPKNQ 99
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BLAST of mRNA_Ecto-sp13_S_contig12217.1868.1 vs. uniprot
Match: A0A7R9WMP4_9STRA (Hypothetical protein n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A7R9WMP4_9STRA)

HSP 1 Score: 107 bits (267), Expect = 1.300e-26
Identity = 55/99 (55.56%), Postives = 69/99 (69.70%), Query Frame = 1 
Query:    1 MHTAWVRVNAVVFFGLTVLLVLSVLTALSTYLHQGF--PDVRKLGVSKFRTFRKSEGTDRLLLNIDVDADLAPAFNWNVKQLFAFVVAEYETPTNPVNQ 291
            MHT WVR+NAVVFFGLTVLL LS L A+S   H  +  P +  L + K R+ +   G DR LL+ D+ AD+APAF+WN+KQLF +VVA YETP   +NQ
Sbjct:   67 MHTLWVRLNAVVFFGLTVLLGLSCLAAVSKIGHSLYYKPVIHNLEIRKLRSLKSHGGVDRALLSFDIHADMAPAFHWNIKQLFVYVVASYETPNKKINQ 165
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig12217.1868.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FLF7_ECTSI5.210e-67100.00Probable Spc3 homolog, subunit of Signal Peptidase... [more]
A0A6V1NT77_HETAK1.710e-3663.92Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A835YJ42_9STRA8.740e-3661.86Signal peptidase 22kDa subunit n=1 Tax=Tribonema m... [more]
A0A7S2XZ74_9STRA9.090e-3358.76Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A7S2SN40_9STRA6.490e-3159.79Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A7S4J3F9_9STRA2.970e-2958.59Hypothetical protein n=1 Tax=Odontella aurita TaxI... [more]
A0A7R9UGA1_9STRA3.040e-2961.22Hypothetical protein n=1 Tax=Pinguiococcus pyrenoi... [more]
A0A7S2XM83_9STRA4.080e-2858.59Hypothetical protein (Fragment) n=1 Tax=Attheya se... [more]
B8CCG8_THAPS2.730e-2755.56Uncharacterized protein n=1 Tax=Thalassiosira pseu... [more]
A0A7R9WMP4_9STRA1.300e-2655.56Hypothetical protein n=1 Tax=Craspedostauros austr... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig12217contigEcto-sp13_S_contig12217:2044..4367 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score206.8
Seed ortholog evalue4.9e-51
Seed eggNOG ortholog2880.D7FLF7
Preferred nameSPCS3
Model size318
KEGG koko:K12948
KEGG Pathwayko03060,map03060
Hectar predicted targeting categorysignal peptide
GOsGO:0003674,GO:0003824,GO:0005575,GO:0005618,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005787,GO:0005789,GO:0005911,GO:0006465,GO:0006508,GO:0006518,GO:0006605,GO:0006807,GO:0006810,GO:0006886,GO:0008104,GO:0008150,GO:0008152,GO:0008233,GO:0009506,GO:0009987,GO:0010467,GO:0012505,GO:0015031,GO:0015833,GO:0016020,GO:0016485,GO:0016787,GO:0019538,GO:0030054,GO:0030154,GO:0030312,GO:0031984,GO:0032502,GO:0032991,GO:0033036,GO:0033365,GO:0034613,GO:0034641,GO:0042175,GO:0042886,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043603,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0045047,GO:0045184,GO:0045444,GO:0046907,GO:0048869,GO:0051179,GO:0051234,GO:0051604,GO:0051641,GO:0051649,GO:0055044,GO:0070727,GO:0070972,GO:0071702,GO:0071704,GO:0071705,GO:0071944,GO:0072594,GO:0072599,GO:0098796,GO:0098827,GO:0140096,GO:1901564,GO:1902494,GO:1905368
Exons2
EggNOG free text desc.signal peptide processing
EggNOG OGsKOG3372@1,KOG3372@2759
Ec32 ortholog descriptionProbable Spc3 homolog, subunit of Signal Peptidase Complex
Ec32 orthologEc-12_007990.1
Cds size318
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462649.666432-CDS-Ecto-sp13_S_contig12217:2043..21461681462649.666432-CDS-Ecto-sp13_S_contig12217:2043..2146Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig12217 2044..2146 +
1681462649.6815681-CDS-Ecto-sp13_S_contig12217:4152..43671681462649.6815681-CDS-Ecto-sp13_S_contig12217:4152..4367Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig12217 4153..4367 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig12217.1868.1prot_Ecto-sp13_S_contig12217.1868.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig12217 2044..4367 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig12217.1868.1

>prot_Ecto-sp13_S_contig12217.1868.1 ID=prot_Ecto-sp13_S_contig12217.1868.1|Name=mRNA_Ecto-sp13_S_contig12217.1868.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=106bp
MHTAWVRVNAVVFFGLTVLLVLSVLTALSTYLHQGFPDVRKLGVSKFRTF
RKSEGTDRLLLNIDVDADLAPAFNWNVKQLFAFVVAEYETPTNPVNQASQ
SVRFA*
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mRNA from alignment at Ecto-sp13_S_contig12217:2044..4367+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig12217.1868.1 ID=mRNA_Ecto-sp13_S_contig12217.1868.1|Name=mRNA_Ecto-sp13_S_contig12217.1868.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2324bp|location=Sequence derived from alignment at Ecto-sp13_S_contig12217:2044..4367+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGCATACAGCATGGGTTCGTGTAAATGCGGTGGTCTTCTTTGGGCTCAC
CGTTCTTCTTGTGTTGAGTGTGCTCACGGCGCTGAGTACATATCTCCACC
AAGGTATGCCGGTCCTTTGCCAGCCAGCTCCTTCAGCTGTTGGAAATCGC
GGTGTTCATAGGTCTTAGTCAAGTTGGTGAGACCTCTGTACAGTAAGACT
CGCTAGGCACGAAAGATGCGAAAATGACTGACTTAGCTGAAGCGCGCGAG
GCATTCATTTGGGTAGTGCTGTGTGGGACAGTGGGATCTTGATTTTTCCG
CTGTCGGCCCCGTTAAACAGTTAAATTTGATGATGCGATCATGTGCAAGC
AGCCGCAGCACCATTGTAGCACCACACAGCCGATAAATACGAAGATCGAG
TGATTTAAGGTAATTTGGATGATGCCACACTTCTCAAGGCCAGCACTCCT
CTGTCGAGTTCGGCGCGTAAGCCGCAAGGATTTATCTACTTGGGCCAGTT
TTGCGGGCGTGACACGTGTCAAGTACGAGGACTACCAAATTCCAGCATGC
ACCACACCCTGCTCACTGCACCCTCGTTTCTGTGACTCTCACCCATGTCG
CGTCTGTGGTCTTGATGTAGTGGTCAGCCAAATCTATCCATCCGCGAAAG
GACGATGCAAACTTGAAAGCGGGCAAATTTTCAGAATTCTGCGGTTGACG
AAATGGCTACCTTTTTTTGTCCTGGCAAGGTTCGTCTTACAGCAACCCCT
CTCTGCGTAATGCCGAACGTTTACGATTTCCAGTCATCCGCTATGGCTCC
GCTGTAGATATGATCCGTTGTTATGCATCCGCCTTGTACTGTCTTCATGG
TCGATGCCGGAAGCCGTTGCATATTGTAAAAATGGAGGAAAGCTGTTGCC
GTGTCGTATTTTTCGTTTTGCTTGCAATCTCACTGCGAAGGTGCGAACTG
CAGCGTATGAACTTGATGTTGGCTACTGTTTGCCGACCTTTCAAACCGAC
TCCGATCGATGAAGTTGTGTTCATCCGATTCTCGACCGTCCCTTACAGGT
GTCACTTACCCTTCGGCCTTGCCTCATTATTACGAAAGAACTCCTGAGTT
CGACACCGAACTGTGCGTATGGTAAAGTGATCGTGCTTCAATTTACACAC
CTCTCATGGTGGAATTTCACTGCTGTTGCAATTCAGTCGGGACGTAGTTG
TGCCTCCCGATAGCTACAACGAACGCCTCCTGAAGGAGCAGAAAAGACGT
TGGATTAATGTGCGTGATGGCGCAGAAGTTGTCCCATTCGCACCATCAGA
GGATAATCAACAGCTCGCGGTTGGAGCGGAGCAACGCGTGTGTATGGTAA
ATCTCCGATTTGTTGTTCCCCCATCACTTCAACAACTCCTCGGGGAAAGG
GGTCAAACTGTCTCATCATTTTAGGGGGAACTGCCTGTCAAGCACGGCCA
TCTTTTGTCGTAGCGTGACGAGACCGGTGTGACGAAGAGTTACCTTATTT
GCTTCCTCCTCACAACCCGGCAGATCGTAAATCGGTGCACTTTAGATCTT
GTTCTGGTCGAACGAAAGGCGTTCTTTTTTTACATGCCATAATTAGTCGG
CAGTTGCAGAAGGGGCCCCACATCGCGTTGGTTGGAAGAGCCCGTTATAT
AGAGCCTTGTTTTCCCTTTCCTGGGGATGACGGGAAGAAGTGTCGCGCTC
CGCGGCTCATTATTACCTAGCGGTCCGCTCTTTACGGCAAACAGCAGTGT
ACTTGATGGAGGGCACGCTAGTGCAGACGCTTCTGTTGAAGACCCAACAA
AGTGTCAGATGTTTCCTCCATTTGTGAGAGAACCCTGCATCCTTGCTTGT
ACCGCGGCGTACCGCGGCGGTGCTACTCTCCTGGCCCATTGGTAACTCGA
CCGGAATAAATTCATATGTTTGTGTGATTCTTGGAGGTGAGTCATGGACC
GGCTCTAGGATATCCCTAGCACGGCAGTTAATTCGTCAAAATTATTTTGC
GGTGATGGCATACATGATTTGCCGTTTGCACGTTCACTTGCCGCTCCTTG
TTTCTTTTTGGTTTTGCTTAAATCTCCTTCCCGTCTCCCCGTACGCGGTG
ATCTGGCAGGGTTTCCGGACGTGAGGAAGCTAGGGGTGAGCAAATTCAGG
ACATTCCGAAAATCTGAAGGCACGGATCGGTTGCTCCTCAACATCGATGT
TGACGCAGATCTGGCTCCGGCCTTCAACTGGAATGTGAAACAGCTCTTCG
CGTTCGTGGTAGCTGAATACGAGACCCCCACAAACCCTGTAAACCAGGCA
AGTCAATCCGTTCGGTTTGCGTAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig12217:2044..4367+

>mRNA_Ecto-sp13_S_contig12217.1868.1 ID=mRNA_Ecto-sp13_S_contig12217.1868.1|Name=mRNA_Ecto-sp13_S_contig12217.1868.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=318bp|location=Sequence derived from alignment at Ecto-sp13_S_contig12217:2044..4367+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGCATACAGCATGGGTTCGTGTAAATGCGGTGGTCTTCTTTGGGCTCAC
CGTTCTTCTTGTGTTGAGTGTGCTCACGGCGCTGAGTACATATCTCCACC
AAGGGTTTCCGGACGTGAGGAAGCTAGGGGTGAGCAAATTCAGGACATTC
CGAAAATCTGAAGGCACGGATCGGTTGCTCCTCAACATCGATGTTGACGC
AGATCTGGCTCCGGCCTTCAACTGGAATGTGAAACAGCTCTTCGCGTTCG
TGGTAGCTGAATACGAGACCCCCACAAACCCTGTAAACCAGGCAAGTCAA
TCCGTTCGGTTTGCGTAG
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