mRNA_Ecto-sp13_S_contig11996.1702.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig11996.1702.1
Unique NamemRNA_Ecto-sp13_S_contig11996.1702.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig11996.1702.1 vs. uniprot
Match: D7G295_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G295_ECTSI)

HSP 1 Score: 519 bits (1336), Expect = 1.050e-182
Identity = 294/362 (81.22%), Postives = 300/362 (82.87%), Query Frame = 1 
Query:    1 PPISPMAEELIRARDVQHGRHRHGSQQPEEPAQVLPQPRGHDFKFFIHSIFDNNNGRGDPTLVFTRATVGHHNINGYGHNFEEKLTEKDTHAPWDAGTKRVVTKMNMDGMALLVRSEVDACDGTGSTRLVPEAKGRASSXXXXXXXXXXCESTSAKADAKSMEDLLVEQLDGLGLSSXXXXXXGTSEFEPASGLTCEKRGKLLGHDAKVLELKTKNSRYAAEINWKDIYWQLALGGAHALVLGLHNRGTFGAGDMRHHSIGEVKTRAEESGSASMVFLANTLRGITKAAHRIIGAPSTARTRPAAPPTPSAVPGETG---TRGESNPRVAAAAAVVVEYSPGRDSRRICIRLATAADGAAPP 1077
            PPISPM EELIRARDVQHGRHR GS QPEEPAQVLPQPRGHDFKF IHSI D+N G GDPTLVFTRATVGHHNINGYGHNFE+KLTEKD HAPWDAGT RVVTKMNMDGMALLVRSEVDACDGTGSTRLVP  KGRAS XXXXXXXXXX         AKS EDLLVEQL GLGLSS      G SE +PASGLTCEKRG+LLGHDAKVLELKTKNSRYA EI+WKDIYWQL LGGAHALVLGLHNRGTFGAGD +HHSIGEVK RAEESGSASMVFLANTLRGITKAAHRIIGA ST   R                  T GESNPRVAAAAAVVVEYSPGRDSR I IRLATAADGA  P
Sbjct:    2 PPISPMTEELIRARDVQHGRHRDGSPQPEEPAQVLPQPRGHDFKFIIHSIGDSN-GHGDPTLVFTRATVGHHNINGYGHNFEKKLTEKDPHAPWDAGTFRVVTKMNMDGMALLVRSEVDACDGTGSTRLVPAGKGRASXXXXXXXXXXXX--XXXXXXAKSTEDLLVEQLGGLGLSSSSPGGEGKSESKPASGLTCEKRGQLLGHDAKVLELKTKNSRYANEISWKDIYWQLTLGGAHALVLGLHNRGTFGAGDTKHHSIGEVKARAEESGSASMVFLANTLRGITKAAHRIIGAASTTPARXXXXXXXXXXXXXXXXXXTHGESNPRVAAAAAVVVEYSPGRDSRCIRIRLATAADGAVSP 360
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BLAST of mRNA_Ecto-sp13_S_contig11996.1702.1 vs. uniprot
Match: A0A6H5KRV4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KRV4_9PHAE)

HSP 1 Score: 90.5 bits (223), Expect = 5.550e-19
Identity = 62/72 (86.11%), Postives = 64/72 (88.89%), Query Frame = 1 
Query:  313 MNMDGMALLVRSEVDACDGTGSTRLVPEAKGRASSXXXXXXXXXXCESTSAKADAKSMEDLLVEQLDGLGLS 528
            MNMDGMAL VRSEVDACDGTGSTRLVPE  GRASSXXXXXXXXXX  + SAKADAKS EDLLVE+L GLGLS
Sbjct:    1 MNMDGMALYVRSEVDACDGTGSTRLVPEGTGRASSXXXXXXXXXXXXN-SAKADAKSTEDLLVERLGGLGLS 71
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BLAST of mRNA_Ecto-sp13_S_contig11996.1702.1 vs. uniprot
Match: A0A8K0UEH8_9AGAM (Uncharacterized protein n=1 Tax=Cristinia sonorae TaxID=1940300 RepID=A0A8K0UEH8_9AGAM)

HSP 1 Score: 70.9 bits (172), Expect = 1.190e-9
Identity = 58/176 (32.95%), Postives = 84/176 (47.73%), Query Frame = 1 
Query:  223 NGYGHNFEEKLTEKDTHAPWDAGTKRVVTKMNMDGMALLVRSEVDACDGTGSTRLVPEAKGRASSXXXXXXXXXXCESTSAKADAKSMEDLLVEQLDGLGLSSXXXXXXGTSEFEPASGLTCEKRGKLLGHDAKVLELKTKNSRYAAEINWKDIYWQLALGGAHALVLGLHNRGTF 750
            N +G+NFE+  T         +G  R++ K +MDG+AL+VR EVDAC         P  K + ++            ST+  + A S  D L + L GL +SS       T+     + +T  + G  +   A ++EL T++ R AA  NW D Y QL L      VLG+H RG F
Sbjct:  526 NTFGYNFEKAHTTPVAGCEGSSGHHRII-KYDMDGLALVVRFEVDAC--------TPRPKSKVTA------------STNEVSPAVSNVDDLSDLLSGLNVSSQTSATADTTTS--TADITIVRAGYEVPQSA-IMELTTRSERNAANFNWDDSYPQLWLSQTSQHVLGVHARGNF 677
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig11996.1702.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 3
Match NameE-valueIdentityDescription
D7G295_ECTSI1.050e-18281.22Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5KRV4_9PHAE5.550e-1986.11Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A8K0UEH8_9AGAM1.190e-932.95Uncharacterized protein n=1 Tax=Cristinia sonorae ... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig11996contigEcto-sp13_S_contig11996:549..5013 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score511.1
Seed ortholog evalue4e-142
Seed eggNOG ortholog2880.D7G295
Model size1083
Hectar predicted targeting categoryother localisation
Exons7
EggNOG OGs2CIRU@1,2SG1W@2759
Ec32 ortholog descriptionhypothetical protein
Ec32 orthologEc-16_001990.1
Cds size1068
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig11996.1702.1prot_Ecto-sp13_S_contig11996.1702.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig11996 564..5013 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462636.2438977-UTR-Ecto-sp13_S_contig11996:548..5631681462636.2438977-UTR-Ecto-sp13_S_contig11996:548..563Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig11996 549..563 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462636.2534316-CDS-Ecto-sp13_S_contig11996:563..5991681462636.2534316-CDS-Ecto-sp13_S_contig11996:563..599Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11996 564..599 +
1681462636.2689424-CDS-Ecto-sp13_S_contig11996:1504..15761681462636.2689424-CDS-Ecto-sp13_S_contig11996:1504..1576Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11996 1505..1576 +
1681462636.2772722-CDS-Ecto-sp13_S_contig11996:2022..22111681462636.2772722-CDS-Ecto-sp13_S_contig11996:2022..2211Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11996 2023..2211 +
1681462636.2877471-CDS-Ecto-sp13_S_contig11996:2695..29441681462636.2877471-CDS-Ecto-sp13_S_contig11996:2695..2944Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11996 2696..2944 +
1681462636.2982743-CDS-Ecto-sp13_S_contig11996:3345..34371681462636.2982743-CDS-Ecto-sp13_S_contig11996:3345..3437Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11996 3346..3437 +
1681462636.310712-CDS-Ecto-sp13_S_contig11996:3937..40701681462636.310712-CDS-Ecto-sp13_S_contig11996:3937..4070Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11996 3938..4070 +
1681462636.321758-CDS-Ecto-sp13_S_contig11996:4716..50131681462636.321758-CDS-Ecto-sp13_S_contig11996:4716..5013Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11996 4717..5013 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig11996.1702.1

>prot_Ecto-sp13_S_contig11996.1702.1 ID=prot_Ecto-sp13_S_contig11996.1702.1|Name=mRNA_Ecto-sp13_S_contig11996.1702.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=356bp
MAEELIRARDVQHGRHRHGSQQPEEPAQVLPQPRGHDFKFFIHSIFDNNN
GRGDPTLVFTRATVGHHNINGYGHNFEEKLTEKDTHAPWDAGTKRVVTKM
NMDGMALLVRSEVDACDGTGSTRLVPEAKGRASSSGGGGGGGGGCESTSA
KADAKSMEDLLVEQLDGLGLSSSSSGGGGTSEFEPASGLTCEKRGKLLGH
DAKVLELKTKNSRYAAEINWKDIYWQLALGGAHALVLGLHNRGTFGAGDM
RHHSIGEVKTRAEESGSASMVFLANTLRGITKAAHRIIGAPSTARTRPAA
PPTPSAVPGETGTRGESNPRVAAAAAVVVEYSPGRDSRRICIRLATAADG
AAPPS*
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mRNA from alignment at Ecto-sp13_S_contig11996:549..5013+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig11996.1702.1 ID=mRNA_Ecto-sp13_S_contig11996.1702.1|Name=mRNA_Ecto-sp13_S_contig11996.1702.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=4465bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11996:549..5013+ (Ectocarpus species13 EcNAP12_S_4_19m)
CCCCCGATATCGCCCATGGCTGAAGAACTTATTCGCGCTCGTGATGTTCA
GGTGTGTGTGTGTGAGCTGGGAGCATGGCGTACAGTCTACACCTTCGACG
CAAAGGATGCATAATAGTCTGCGCAAGGCAGTTAGTTCCTTGTGCCACAG
ATTTCCATATCACAGCAAACTTTATTTTGTTAAGGGTTTCTGCATCATCT
TGAACTCGTGTAGTGCAAGACGTATGGATCAGTGATGTAAAGTATGCTAC
GTGCCTTGAGTGGATGATGCACATCAGGCACATGTATGTACACGTAGAAG
TCTAAAGCCGGGCCAACCAAATAACAAGCTGTTGTACGAAACGGAAAAAA
CTTGCAAATCCAGCCAATCGGCCCGGTTGGCACCACCGTCGGTCCTGCTC
TTTATGGTATGATCAATATGTGTGTCGTCCCCCTCCCCCCCTACGCGCAA
CCAGCCTTGACCAGAAGGTGTAGCATCAAGTGTTGTACCTGGTCTACCTT
GTCGTTCGTAGAGCACTGTAGTCTACGCACAAGGCAGACAAATGTGGTAC
TTGTTTTGTCGCCAACTTTTTCATCCTGCACCACCAGCTCTATCGCAAAC
AGTAGTAAAGAACATAAATTTACTTCATTTGTAAAACAGTAAGATGTGTG
TTGCTCTAAAAGTTGTGCTTTCCAACCTCTTCGTATGATCGTATCTGTAG
ACTGACCACAGCAACGTGCTCCCCACGGGCACCGAGGTAGTAGCCTCTAA
CAACTGGAGCACGAGGTCGAGAGAGGGGCACCCTCGCATCATCGTCCCTG
GCGCGCCGCGCAATTATGTCAGCTGCATGAGCCGTCACGGGTTCATTTTG
GGTAAGGACGGCGAAGACTAATTTGTGGACGAAAACCGCGCGCGGATGAA
ACCATCCAGCCGCTGTTTCGCTCCCTCGACATCTGCTCCCCCGCTTTCGA
CATCAGCACGGTCGACATCGTCACGGATCGCAACAACCTGAGGAACCTGC
TCAGGTTCTGCCGCAGCCCCGGGGACACGTGAGTTGTGTTTGGTCAACCA
GGCGGCGCAAACAGGGCAACCCCGGTACCATTCCTTTTCTTTACGAGAGG
TTAGCGGGTGGGGGGGTTGCGGAGTCATTCTTTCCACCCGCTCAGTTTTT
TTGTCGTTTTTTCCTGACGAACGTCAACGGTTCTTCTGTCATGTGCGTGT
CGTGGCTCGCTTGATCTACTGCTTGGAGTCGTACTTTGTCCACTTGCTTT
GCTCTTGGGCCCTTGTCCAAGCTTCGGGGTCAGAAAGCGTATGTTACTGG
GGCATGGGAGTCAGCGGGCACTCCGCGCAAGCGCGCATTTCATTGTTTTT
CATTCACATCGAACGGCTCCTGGAGCAATAATTTTTCGACAATTTTTCAC
GCTTCCCCTGCTTCTTGCCGCTCGGCAACGTCGTACAATTTCGCAGCACC
ACGGCCAACGAGTTTGGCAACCAGGACTTCAAGTTCTTCATCCACTCCAT
TTTCGACAACAACAACGGACGCGGCGACCCGACCCTGGTCTTCACGAGGG
CGACGGTAGGCCATCACAACATCAACGGGTACGGCCACAACTTCGAGGAA
AAGCTCACCGAGAAGGATACGCATGCTCCGTGGGACGCCGGGACGAAACG
TGTCGTAACAAAGGTCGAGAACTACTGTAACAAAGTACCTGCCCATCGTT
AGTAGAGTACGCCTTCCGCAACGCATGTACCATGATTTCGAAATATTCTG
GGTGACTACATACGGCATCACCGACGCACATGAAACATGACGGAGACAAC
CTAAGCTAGTTGAATTGAATGTTCTCAGAGCATCACGTATCGCTTATCCC
CTCTAATCGAGCATCACAGACAACGTCCCGTTCAAGATATTGGTCAGAGG
GGGACGAAGTGAAGTCTTCGTCTTCACAACGATCTTTTTTACCCTCTACA
GATATTTCTCACGCTGCTGGTGAAGTTGGGGTCAGGCTGCTTCCCCCCCC
TTCCTTGCGCGCATGTGTCTCTGACGAAAGTTGAGCAACACGCGACCGAA
GATTTGCGCGTTCCTTACTGCAGAGTTGTCGTCGTTTCTACATCATAGGT
TTTTCATCGAACGCGCCCTTCCCCGTTGCGTGTGTAACCCTCCCCAGATG
AACATGGATGGAATGGCGCTGCTTGTGCGCTCTGAGGTGGACGCCTGCGA
CGGGACTGGTTCCACTAGGCTCGTTCCTGAGGCGAAGGGGCGCGCCTCAT
CTTCCGGTGGTGGTGGTGGTGGTGGTGGTGGTTGTGAAAGTACCTCTGCC
AAGGCTGACGCCAAGAGTATGGAAGACCTGCTGGTTGAACAGCTCGATGG
CCTGGGGCTCTCTTCTTCCTCGTCGGGAGGAGGAGGAACGAGTGAGGTGG
GCCGACAGACAAATTCGTGATTGCTTGCTGTTGTCTTCCACATGGAAAAG
GAAAATATTGAAACGGACAGGGAGCGTCCAGGCTTACTTAACAAGCCTAA
GATATTTTTTCGATTCGAGTACCACACATGGGGGGGGGAAGTCAATCCCA
TAGAAGCAAGCAACGTATTTCTGTCATTTACACAACATCGGCGCCAGCTC
TCCTTCGGCCTCGAACCACAGCGTCGATTGTATTTGCACTTTATCCCGTC
GAAGAACTTAAAGGCCTACAAACAAGCGGGTAGCTAGCCTAGTCACTAAC
ACAACCACCCGTACCATTACATGAGTCCTTCCTGTTTTCGTTGTTTGTTC
GCCTCCTCCCTCCCCTCCCCTCTCGGGCATGTACGCTACGGTCAAAGTTT
GAGCCTGCCTCTGGTCTGACGTGCGAAAAGAGGGGGAAGCTTCTCGGACA
CGACGCCAAGGTTTTGGAGCTCAAGACCAAAAACTCACGGTATGATGCGT
CGTCTTCTTGTTCGTGCTTCTTAACAACACATGAAACATAATTTCAGAGG
AATAATCTGTATGCCTGGGAGGCACGGGCTGCACGGCTTGACCGAAGCTC
GTTGGGGGCAGAAAACCTGCGCTATTTCAGCAATCAACACCGCTATTGCG
AAAACAAGGCTGGTCATTTGTGTTCTAAGGATACCCGAGGGTTGAAAAGA
CGGGAAGTGACATCGATCGGAAATTATAGACTAGGGAAAACATCTCTTGA
CCCACTTTCGGGAAGGCACGATTGGCCATCGATGATCGGTCCTGTGGTAG
CGATGCTCCGTCCTCTCGAGGACGCGTGGCCAATGGCCTAGCCGCCAAGG
CATGCAAAACATAAGAGTACGCCCCCCCCCCCCCCCCCCCCCCCCCCTCC
CCTTCCCCCGCCGCCCCCCCCTTCCTCCCCCCCCCTCTCCCACTCCCCCC
ACCGCCCGCGGGGCCAACCCCATGCATGCCGTCCCGAAGGTACGCCGCGG
AGATCAACTGGAAGGACATCTACTGGCAGCTGGCCCTGGGCGGCGCTCAC
GCGCTGGTCCTCGGCCTACACAACCGCGGGACCTTTGGGGCCGGGGACAT
GAGGCACCATTCCATCGGCGAGGTGTGGGACAACCCTACGAAAAACACCT
TGAACGAGAACACACATGATGCGCCTCAGGCGTAACGTTATTTTTTCTTG
GTCTACAAGAATGCCGCCGTGAAGCCAATGATATCTATACGCGTACCGAA
ATTTCTTGTTGAGAAAGCAAGCACCGAACGCCCTTCGATATGAAGCGGTT
TCGGTTGTGTCGTATTTGGATTCGAGATAGGGTTATCGCGCCCGGCGTGG
AGTGGCATCGATGCTCTCTCCCGGTTGAACCCATTCTTTTCCTTTGCGAT
GTTCCACCTGTAAATGATGGCTTGTTTTTGCGCCATGGTGAACGCATCGA
CCGATGTACTTTACGCAGTTACTTCTGAGCACATGTGCCCGAAAACAAGG
GACGGCGCGACTCGAAAACGTCCAACAGGAGACTGCGGTTCTTCGTAGAA
CATTTATTTCAAGCATTGACATTGTGCTTGAGGATCGACGACGACCTTCC
CCAGGAAAAGGATAAACTGTTATTGACGACCAAACAAACACAAGCAAAAA
TTTAGCAAGCATTGTTCCACCATAACGTGCAGACAACCACGTCATCGTGT
ATAAATATGTTCGAGCCGTCACATCTAAATATTTTCTGCTATTACGCATA
CGACTGTCCGTTCACTAGGTCAAGACTCGCGCGGAAGAGTCCGGGTCGGC
GTCCATGGTGTTCCTAGCGAACACCTTGCGCGGTATCACCAAGGCCGCGC
ACCGCATCATCGGTGCCCCGTCTACTGCGCGCACGCGCCCGGCTGCGCCT
CCTACGCCGTCCGCTGTTCCTGGCGAGACGGGAACACGCGGCGAATCAAA
CCCCCGCGTCGCCGCCGCCGCTGCCGTCGTCGTCGAGTACTCGCCTGGCC
GCGACAGCAGGCGCATCTGCATCAGGCTTGCCACCGCCGCGGATGGGGCC
GCTCCTCCCTCCTGA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig11996:549..5013+

>mRNA_Ecto-sp13_S_contig11996.1702.1 ID=mRNA_Ecto-sp13_S_contig11996.1702.1|Name=mRNA_Ecto-sp13_S_contig11996.1702.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=1068bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11996:549..5013+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGGCTGAAGAACTTATTCGCGCTCGTGATGTTCAGCACGGTCGACATCG
TCACGGATCGCAACAACCTGAGGAACCTGCTCAGGTTCTGCCGCAGCCCC
GGGGACACGACTTCAAGTTCTTCATCCACTCCATTTTCGACAACAACAAC
GGACGCGGCGACCCGACCCTGGTCTTCACGAGGGCGACGGTAGGCCATCA
CAACATCAACGGGTACGGCCACAACTTCGAGGAAAAGCTCACCGAGAAGG
ATACGCATGCTCCGTGGGACGCCGGGACGAAACGTGTCGTAACAAAGATG
AACATGGATGGAATGGCGCTGCTTGTGCGCTCTGAGGTGGACGCCTGCGA
CGGGACTGGTTCCACTAGGCTCGTTCCTGAGGCGAAGGGGCGCGCCTCAT
CTTCCGGTGGTGGTGGTGGTGGTGGTGGTGGTTGTGAAAGTACCTCTGCC
AAGGCTGACGCCAAGAGTATGGAAGACCTGCTGGTTGAACAGCTCGATGG
CCTGGGGCTCTCTTCTTCCTCGTCGGGAGGAGGAGGAACGAGTGAGTTTG
AGCCTGCCTCTGGTCTGACGTGCGAAAAGAGGGGGAAGCTTCTCGGACAC
GACGCCAAGGTTTTGGAGCTCAAGACCAAAAACTCACGGTACGCCGCGGA
GATCAACTGGAAGGACATCTACTGGCAGCTGGCCCTGGGCGGCGCTCACG
CGCTGGTCCTCGGCCTACACAACCGCGGGACCTTTGGGGCCGGGGACATG
AGGCACCATTCCATCGGCGAGGTCAAGACTCGCGCGGAAGAGTCCGGGTC
GGCGTCCATGGTGTTCCTAGCGAACACCTTGCGCGGTATCACCAAGGCCG
CGCACCGCATCATCGGTGCCCCGTCTACTGCGCGCACGCGCCCGGCTGCG
CCTCCTACGCCGTCCGCTGTTCCTGGCGAGACGGGAACACGCGGCGAATC
AAACCCCCGCGTCGCCGCCGCCGCTGCCGTCGTCGTCGAGTACTCGCCTG
GCCGCGACAGCAGGCGCATCTGCATCAGGCTTGCCACCGCCGCGGATGGG
GCCGCTCCTCCCTCCTGA
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