mRNA_Ecto-sp13_S_contig11676.1458.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig11676.1458.1
Unique NamemRNA_Ecto-sp13_S_contig11676.1458.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig11676.1458.1 vs. uniprot
Match: D8LKW5_ECTSI (Chorismate synthase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LKW5_ECTSI)

HSP 1 Score: 281 bits (718), Expect = 1.730e-91
Identity = 143/146 (97.95%), Postives = 145/146 (99.32%), Query Frame = 1 
Query:    1 QIGSGFAGTLLSGKTHNDEFYVDEEGQTRTRTNRSGGVQGGISNGETIVVRVAFKPTSTIGQLQSTVTRGGEETTMRGKGRHDPCVLPRAAPMVEAMVALVLADVLMQQKAQCELFPADALETLETNPLGKKLSGAGVSPASVNTP 438
            +IGSGFAGTLLSGKTHNDEFYVDEEGQTRTRTNRSGGVQGGISNGETIVVRVAFKPTSTIGQLQ+TVTRGGEETTMRGKGRHDPCVLPRAAPMVEAMVALVLADVLMQQKAQCELFPADALETLETNPLGKKLSGAGVS ASVNTP
Sbjct:  292 EIGSGFAGTLLSGKTHNDEFYVDEEGQTRTRTNRSGGVQGGISNGETIVVRVAFKPTSTIGQLQNTVTRGGEETTMRGKGRHDPCVLPRAAPMVEAMVALVLADVLMQQKAQCELFPADALETLETNPLGKKLSGAGVSAASVNTP 437
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BLAST of mRNA_Ecto-sp13_S_contig11676.1458.1 vs. uniprot
Match: A0A835Z9B0_9STRA (Chorismate synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z9B0_9STRA)

HSP 1 Score: 223 bits (569), Expect = 1.350e-69
Identity = 107/139 (76.98%), Postives = 123/139 (88.49%), Query Frame = 1 
Query:    1 QIGSGFAGTLLSGKTHNDEFYVDEEGQTRTRTNRSGGVQGGISNGETIVVRVAFKPTSTIGQLQSTVTRGGEETTMRGKGRHDPCVLPRAAPMVEAMVALVLADVLMQQKAQCELFPADALETLETNPLGKKLSGAGVS 417
            ++GSGFAGTLL+G+ HNDEFY+DE+G+TRTRTNRSGG+QGGISNGETI++RVAFKPTSTIGQ Q TVTR G ET MRGKGRHDPCVLPRA PMVEAM+ALVLAD LM   AQC+LFPA AL++++ NPLGKKL G G S
Sbjct:  251 EVGSGFAGTLLTGRQHNDEFYIDEDGRTRTRTNRSGGIQGGISNGETIIIRVAFKPTSTIGQEQETVTREGVETKMRGKGRHDPCVLPRAVPMVEAMMALVLADALMAHAAQCQLFPAGALDSVDANPLGKKLEGLGAS 389
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BLAST of mRNA_Ecto-sp13_S_contig11676.1458.1 vs. uniprot
Match: A0A7S2FB41_9STRA (Chorismate synthase n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2FB41_9STRA)

HSP 1 Score: 200 bits (508), Expect = 7.320e-63
Identity = 100/135 (74.07%), Postives = 112/135 (82.96%), Query Frame = 1 
Query:    1 QIGSGFAGTLLSGKTHNDEFYVDEEGQTRTRTNRSGGVQGGISNGETIVVRVAFKPTSTIGQLQSTVTRGGEETTMRGKGRHDPCVLPRAAPMVEAMVALVLADVLMQQKAQCELFPADALETLETNPLGKKLSG 405
            +IGSGF GTLL+GK HNDEFYVDE G+TRT+TNRSGG+QGGISNGETI++RVAFKPTSTIGQ Q TVTR GEET MRGKGRHDPCVLPRA PMVE+MVALV+AD +MQQ AQC L   +       NPLGK + G
Sbjct:   21 EIGSGFGGTLLTGKEHNDEFYVDENGRTRTKTNRSGGIQGGISNGETIIIRVAFKPTSTIGQEQETVTRTGEETLMRGKGRHDPCVLPRACPMVESMVALVMADHIMQQHAQCNLLAVEG--DSGANPLGKLVEG 153
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BLAST of mRNA_Ecto-sp13_S_contig11676.1458.1 vs. uniprot
Match: A0A7S2B041_9STRA (Chorismate synthase n=2 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2B041_9STRA)

HSP 1 Score: 206 bits (525), Expect = 1.740e-62
Identity = 100/134 (74.63%), Postives = 112/134 (83.58%), Query Frame = 1 
Query:    1 QIGSGFAGTLLSGKTHNDEFYVDEEGQTRTRTNRSGGVQGGISNGETIVVRVAFKPTSTIGQLQSTVTRGGEETTMRGKGRHDPCVLPRAAPMVEAMVALVLADVLMQQKAQCELFPADALETLETNPLGKKLS 402
            ++GSGF GT+L+GK HNDEFY+DE GQTRTR+NRSGG+QGGISNGETI++RVAFKPTSTIGQLQ TVTRGGEETTMRGKGRHDPCVLPRA PMVE+MVAL L D LM   AQC+L P         NPLGK L+
Sbjct:  298 EVGSGFGGTMLTGKEHNDEFYIDENGQTRTRSNRSGGIQGGISNGETIILRVAFKPTSTIGQLQETVTRGGEETTMRGKGRHDPCVLPRAVPMVESMVALTLVDHLMMHHAQCQLLPLPMENEEAPNPLGKALN 431
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BLAST of mRNA_Ecto-sp13_S_contig11676.1458.1 vs. uniprot
Match: F0YC54_AURAN (Chorismate synthase n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YC54_AURAN)

HSP 1 Score: 204 bits (519), Expect = 4.810e-62
Identity = 109/145 (75.17%), Postives = 120/145 (82.76%), Query Frame = 1 
Query:    1 QIGSGFAGTLLSGKTHNDEFYVDEEGQTRTRTNRSGGVQGGISNGETIVVRVAFKPTSTIGQLQSTVTRGGEETTMRGKGRHDPCVLPRAAPMVEAMVALVLADVLMQQKAQCEL--FPADALETLETNPLGKK-LSGAGVSPAS 426
            ++GSGF GTLL+GK HNDEFYVD +G+TRTR+NRSGG+QGGISNGETIVVRVAFKPTSTIGQLQ+TVTR GEE  +RGKGRHDPCVLPRAAPMVE+MVAL L D LMQQKAQCEL  F AD       NPLG K +   GV PAS
Sbjct:  252 EVGSGFDGTLLAGKDHNDEFYVDADGRTRTRSNRSGGIQGGISNGETIVVRVAFKPTSTIGQLQATVTRDGEEVELRGKGRHDPCVLPRAAPMVESMVALTLMDALMQQKAQCELLDFEADG----GFNPLGSKPVHDLGVVPAS 392
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BLAST of mRNA_Ecto-sp13_S_contig11676.1458.1 vs. uniprot
Match: A0A8J2SL65_9STRA (Chorismate synthase n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SL65_9STRA)

HSP 1 Score: 198 bits (504), Expect = 1.800e-59
Identity = 98/115 (85.22%), Postives = 107/115 (93.04%), Query Frame = 1 
Query:    1 QIGSGFAGTLLSGKTHNDEFYVDEEGQTRTRTNRSGGVQGGISNGETIVVRVAFKPTSTIGQLQSTVTRGGEETTMRGKGRHDPCVLPRAAPMVEAMVALVLADVLMQQKAQCEL 345
            +IGSGF GTLL+GK HNDEFYVD  G+TRTR+NRSGGVQGGISNGETI VRVAFKPTSTIGQLQ+TVTR G+ET +RGKGRHDPCVLPRAAPMVE+MVALVLAD LMQQKAQC+L
Sbjct:  283 EIGSGFGGTLLAGKDHNDEFYVDANGRTRTRSNRSGGVQGGISNGETIWVRVAFKPTSTIGQLQNTVTRDGQETELRGKGRHDPCVLPRAAPMVESMVALVLADALMQQKAQCDL 397
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BLAST of mRNA_Ecto-sp13_S_contig11676.1458.1 vs. uniprot
Match: A0A7S2XR93_9STRA (Chorismate synthase n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2XR93_9STRA)

HSP 1 Score: 194 bits (492), Expect = 2.360e-59
Identity = 100/147 (68.03%), Postives = 112/147 (76.19%), Query Frame = 1 
Query:    1 QIGSGFAGTLLSGKTHNDEFYVDEE-GQTRTRTNRSGGVQGGISNGETIVVRVAFKPTSTIGQLQSTVTRGGEETTMRGKGRHDPCVLPRAAPMVEAMVALVLADVLMQQKAQCELFPADALETLETNPLGKKLSGAGVSPASVNTP 438
            + G GFAGTLLSGK HNDEFYVD E G TRT TNRSGG+QGGISNGE +V+ VAFKPTSTIGQ+Q TVTR G E  +RGKGRHDPCVLPRA PMVEAM+ALVL D LM Q+AQCELFP +A    + NP+G      G  P  +N P
Sbjct:  118 ESGDGFAGTLLSGKDHNDEFYVDPETGATRTSTNRSGGIQGGISNGENVVIHVAFKPTSTIGQVQKTVTRDGREVELRGKGRHDPCVLPRAVPMVEAMMALVLVDALMMQQAQCELFPNEASSEEKPNPMGTTARYEG-EPRHMNEP 263
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BLAST of mRNA_Ecto-sp13_S_contig11676.1458.1 vs. uniprot
Match: A0A7S3P6R5_9STRA (Chorismate synthase n=1 Tax=Amphora coffeiformis TaxID=265554 RepID=A0A7S3P6R5_9STRA)

HSP 1 Score: 190 bits (483), Expect = 2.680e-59
Identity = 98/144 (68.06%), Postives = 110/144 (76.39%), Query Frame = 1 
Query:    1 QIGSGFAGTLLSGKTHNDEFYVDEE-GQTRTRTNRSGGVQGGISNGETIVVRVAFKPTSTIGQLQSTVTRGGEETTMRGKGRHDPCVLPRAAPMVEAMVALVLADVLMQQKAQCELFPADALETLETNPLGKKLSGAGVSPASV 429
            + G GFAGTLL+GK HNDEFY+D E G TRT+TNRSGG+QGGISNGE IVV VAFKPTSTIGQ+Q TVTR G+E  +RGKGRHDPCVLPRA PMVEAMVAL L D LM Q+AQCELF  +A      NP+G      G +P  V
Sbjct:   23 ESGDGFAGTLLTGKDHNDEFYIDPETGATRTKTNRSGGIQGGISNGENIVVHVAFKPTSTIGQVQKTVTRDGKEVELRGKGRHDPCVLPRAVPMVEAMVALTLVDHLMMQQAQCELFEIEAPLADRPNPMGTTAHREGGAPVQV 166
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BLAST of mRNA_Ecto-sp13_S_contig11676.1458.1 vs. uniprot
Match: A0A7S0SX69_9STRA (Chorismate synthase n=1 Tax=Chromulina nebulosa TaxID=96789 RepID=A0A7S0SX69_9STRA)

HSP 1 Score: 197 bits (500), Expect = 5.720e-59
Identity = 100/139 (71.94%), Postives = 112/139 (80.58%), Query Frame = 1 
Query:    1 QIGSGFAGTLLSGKTHNDEFYVDEEGQTRTRTNRSGGVQGGISNGETIVVRVAFKPTSTIGQLQSTVTRGGEETTMRGKGRHDPCVLPRAAPMVEAMVALVLADVLMQQKAQCELFPADAL-----ETLETNPLGKKLS 402
            +IGSGF GTLLSG  HNDEFY DE G+ RTR+NRSGG+QGGISNGE I++RVAFKPTSTIG+ Q+TVTR G  TT+RGKGRHDPCVLPRA PMVEAMVALVL D LMQQ AQCELFP D        ++  N LGKKL+
Sbjct:  274 EIGSGFGGTLLSGYDHNDEFYSDESGRIRTRSNRSGGIQGGISNGENIILRVAFKPTSTIGREQNTVTREGNSTTLRGKGRHDPCVLPRAVPMVEAMVALVLLDALMQQNAQCELFPRDKFVDSDSNSISENILGKKLA 412
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BLAST of mRNA_Ecto-sp13_S_contig11676.1458.1 vs. uniprot
Match: A0A7S0FMT2_9STRA (Chorismate synthase n=1 Tax=Minutocellus polymorphus TaxID=265543 RepID=A0A7S0FMT2_9STRA)

HSP 1 Score: 191 bits (484), Expect = 6.080e-59
Identity = 97/131 (74.05%), Postives = 107/131 (81.68%), Query Frame = 1 
Query:    1 QIGSGFAGTLLSGKTHNDEFYVDEE-GQTRTRTNRSGGVQGGISNGETIVVRVAFKPTSTIGQLQSTVTRGGEETTMRGKGRHDPCVLPRAAPMVEAMVALVLADVLMQQKAQCELFPADALETLETNPLG 390
            + G GFAGTLL+GK HNDEFY+D E G TRTRTNRSGG+QGGISNGE IVV VAFKPTSTIGQ Q+TVTR G+E  +RGKGRHDPCVLPRA PMVEAMVAL L D LM Q+AQCELFP +A      NP+G
Sbjct:   58 ESGDGFAGTLLTGKDHNDEFYIDPETGATRTRTNRSGGIQGGISNGEDIVVHVAFKPTSTIGQAQNTVTRDGQEVELRGKGRHDPCVLPRAVPMVEAMVALTLVDHLMMQQAQCELFPNEAAVEDLPNPMG 188
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig11676.1458.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LKW5_ECTSI1.730e-9197.95Chorismate synthase n=2 Tax=Ectocarpus TaxID=2879 ... [more]
A0A835Z9B0_9STRA1.350e-6976.98Chorismate synthase n=1 Tax=Tribonema minus TaxID=... [more]
A0A7S2FB41_9STRA7.320e-6374.07Chorismate synthase n=1 Tax=Florenciella parvula T... [more]
A0A7S2B041_9STRA1.740e-6274.63Chorismate synthase n=2 Tax=Dictyocha speculum Tax... [more]
F0YC54_AURAN4.810e-6275.17Chorismate synthase n=1 Tax=Aureococcus anophageff... [more]
A0A8J2SL65_9STRA1.800e-5985.22Chorismate synthase n=1 Tax=Pelagomonas calceolata... [more]
A0A7S2XR93_9STRA2.360e-5968.03Chorismate synthase n=1 Tax=Attheya septentrionali... [more]
A0A7S3P6R5_9STRA2.680e-5968.06Chorismate synthase n=1 Tax=Amphora coffeiformis T... [more]
A0A7S0SX69_9STRA5.720e-5971.94Chorismate synthase n=1 Tax=Chromulina nebulosa Ta... [more]
A0A7S0FMT2_9STRA6.080e-5974.05Chorismate synthase n=1 Tax=Minutocellus polymorph... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig11676contigEcto-sp13_S_contig11676:3894..4957 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score283.1
Seed ortholog evalue7.4e-74
Seed eggNOG ortholog2880.D8LKW5
Model size441
KEGG rclassRC00586
KEGG koko:K01736
KEGG ReactionR01714
KEGG Pathwayko00400,ko01100,ko01110,ko01130,ko01230,map00400,map01100,map01110,map01130,map01230
KEGG ModuleM00022
Hectar predicted targeting categoryother localisation
GOsGO:0000166,GO:0003674,GO:0003824,GO:0004107,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006082,GO:0006520,GO:0006725,GO:0006807,GO:0008150,GO:0008152,GO:0008652,GO:0009058,GO:0009072,GO:0009073,GO:0009423,GO:0009507,GO:0009536,GO:0009987,GO:0010181,GO:0016053,GO:0016829,GO:0016835,GO:0016838,GO:0019438,GO:0019752,GO:0032553,GO:0036094,GO:0043167,GO:0043168,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0043648,GO:0043650,GO:0044237,GO:0044238,GO:0044249,GO:0044281,GO:0044283,GO:0044424,GO:0044444,GO:0044464,GO:0046394,GO:0046417,GO:0048037,GO:0050662,GO:0071704,GO:0097159,GO:0097367,GO:1901265,GO:1901360,GO:1901362,GO:1901363,GO:1901564,GO:1901566,GO:1901576
Exons3
EggNOG free text desc.chorismate synthase activity
EggNOG OGsCOG0082@1,KOG4492@2759
EC4.2.3.5
Cds size441
COG Functional cat.E
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462615.2384627-CDS-Ecto-sp13_S_contig11676:3893..40491681462615.2384627-CDS-Ecto-sp13_S_contig11676:3893..4049Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11676 3894..4049 -
1681462615.2488868-CDS-Ecto-sp13_S_contig11676:4454..45831681462615.2488868-CDS-Ecto-sp13_S_contig11676:4454..4583Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11676 4455..4583 -
1681462615.2625818-CDS-Ecto-sp13_S_contig11676:4801..49571681462615.2625818-CDS-Ecto-sp13_S_contig11676:4801..4957Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11676 4802..4957 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig11676.1458.1prot_Ecto-sp13_S_contig11676.1458.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig11676 3894..4957 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig11676.1458.1

>prot_Ecto-sp13_S_contig11676.1458.1 ID=prot_Ecto-sp13_S_contig11676.1458.1|Name=mRNA_Ecto-sp13_S_contig11676.1458.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=147bp
QIGSGFAGTLLSGKTHNDEFYVDEEGQTRTRTNRSGGVQGGISNGETIVV
RVAFKPTSTIGQLQSTVTRGGEETTMRGKGRHDPCVLPRAAPMVEAMVAL
VLADVLMQQKAQCELFPADALETLETNPLGKKLSGAGVSPASVNTP*
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mRNA from alignment at Ecto-sp13_S_contig11676:3894..4957-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig11676.1458.1 ID=mRNA_Ecto-sp13_S_contig11676.1458.1|Name=mRNA_Ecto-sp13_S_contig11676.1458.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=1064bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11676:3894..4957- (Ectocarpus species13 EcNAP12_S_4_19m)
CAGATCGGATCGGGATTCGCTGGCACCCTCCTGTCGGGGAAGACCCACAA
CGATGAGTTTTACGTCGACGAGGAAGGGCAAACGCGCACGCGCACAAACC
GCAGCGGGGGAGTTCAGGGGGGCATCAGCAACGGGGAGACAATAGTGGTT
AGAGTGGTGAGTCAAAATGGCGATGGCGTGGAGATGGCGATGGCATCTGT
GTGGTTGTCACCCCCCTTAGCCCCCCCCGTGTACGACGAAGACATATATC
GTGTCTCGACGCGTCGCACGTCGTGTTGGAGAACTGCGTGCTGCTTCCTT
CTCGCCTGTAGCACACGCAACTGCATCTGTCTCTGCTTCCGTTCAGTCAA
CCCCACGCAATCGCTAATCATCAGGCATTCAAGCCGACGTCCACCATCGG
GCAGCTTCAAAGTACGGTCACGAGGGGGGGAGAGGAGACGACCATGAGGG
GAAAGGGTCGCCATGACCCCTGCGTTCTTCCTCGGGCAGCTCCCATGGTC
GAGGTAAGTTCGGCGGTGTCGCGCCTCGAGTTGACGTACACTGTTGAAAC
GAGTTATTTTCGTGTCTCAACTCTGTTGACGTCCACCAGTCTCATCACCA
CGGAGGCATTCCGACGCGGTCTCGAGCACCCCACGGGTTTCAAGAGTCTC
AGTTGTTGGTCATCCTCGGTAGGTCGTGGATTTTACGGACTCGTGCTCCC
TCCTACCCCCTTCCCTTGTTTTCAAAGTGCCATCACGGTGACGCCGCGCA
AATACTTACACCTTGCTCGCTTGTAGTTTTTATGGCAATCTTTTGTCCGC
ACTCCCGAATATTATGTCGTGCCCCGGAAGCTGCCCGCATGAAGTTGATT
TAGGTCGGCCGTACCCTTAACCGTCCCCTTCGTGGTATGTCCTTGGTGTG
GTTCACAGGCGATGGTTGCCTTAGTCCTTGCGGACGTCTTGATGCAGCAA
AAGGCGCAGTGCGAGCTGTTCCCAGCTGACGCGTTGGAGACTCTGGAGAC
GAACCCTTTGGGGAAGAAATTGTCTGGGGCGGGGGTATCTCCTGCTTCGG
TAAACACACCATGA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig11676:3894..4957-

>mRNA_Ecto-sp13_S_contig11676.1458.1 ID=mRNA_Ecto-sp13_S_contig11676.1458.1|Name=mRNA_Ecto-sp13_S_contig11676.1458.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=441bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11676:3894..4957- (Ectocarpus species13 EcNAP12_S_4_19m)
CAGATCGGATCGGGATTCGCTGGCACCCTCCTGTCGGGGAAGACCCACAA
CGATGAGTTTTACGTCGACGAGGAAGGGCAAACGCGCACGCGCACAAACC
GCAGCGGGGGAGTTCAGGGGGGCATCAGCAACGGGGAGACAATAGTGGTT
AGAGTGGCATTCAAGCCGACGTCCACCATCGGGCAGCTTCAAAGTACGGT
CACGAGGGGGGGAGAGGAGACGACCATGAGGGGAAAGGGTCGCCATGACC
CCTGCGTTCTTCCTCGGGCAGCTCCCATGGTCGAGGCGATGGTTGCCTTA
GTCCTTGCGGACGTCTTGATGCAGCAAAAGGCGCAGTGCGAGCTGTTCCC
AGCTGACGCGTTGGAGACTCTGGAGACGAACCCTTTGGGGAAGAAATTGT
CTGGGGCGGGGGTATCTCCTGCTTCGGTAAACACACCATGA
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