mRNA_Ecto-sp13_S_contig11426.1258.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig11426.1258.1
Unique NamemRNA_Ecto-sp13_S_contig11426.1258.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig11426.1258.1 vs. uniprot
Match: D8LH14_ECTSI (PHM7_cyt domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LH14_ECTSI)

HSP 1 Score: 521 bits (1343), Expect = 7.400e-176
Identity = 264/273 (96.70%), Postives = 266/273 (97.44%), Query Frame = 1 
Query:    1 IRQLQEKRSKASRKIIHFAAEAKMAVMMTNKAVAAYVTFDDEMAAVEARVSYSGSWITRLCVKEKMKLKGKRISVEEAPEPSTILWENYGYRRCELWTRRTATLLVGTSFITLSIAMSIYALYQNQQAESEGGTAECPEGWDALSRREKEDAVAEDGTILHCLCDGGDGSAWTLSPNDVCYEYAWAKVQVTWLVLGTSFMVAFTNAAINKITRMMGVFEKHLSVDKQEVAVYRRLVLLKFVNIGLTVLVTNSRRVIDVLGVSLDYEEDFTSEW 819
            IRQLQEKRSKASRKIIHFA EAKMAVMMTNKAVAAYVTFDDEMAAVEARVSYSGSWITRLC KE MKLKGKRISVEEAPEPSTILWENYGYRRCELWTRRTATLL+GTSFITLSI MSIYALYQNQQAESEGGTAECPEGWDALSR E+EDAVAEDGTILHCLCDG DGSAWTLSPNDVCYEYAWAKVQ TWLVLGTSFMVAFTNAAINKITRMMGVFEKHLSVDKQEVAVYRRLVLLKFVNIGLTVLVTNSRRVIDVLGVSLDYEEDFTSEW
Sbjct:  418 IRQLQEKRSKASRKIIHFAMEAKMAVMMTNKAVAAYVTFDDEMAAVEARVSYSGSWITRLCAKENMKLKGKRISVEEAPEPSTILWENYGYRRCELWTRRTATLLIGTSFITLSIGMSIYALYQNQQAESEGGTAECPEGWDALSRWEQEDAVAEDGTILHCLCDGADGSAWTLSPNDVCYEYAWAKVQATWLVLGTSFMVAFTNAAINKITRMMGVFEKHLSVDKQEVAVYRRLVLLKFVNIGLTVLVTNSRRVIDVLGVSLDYEEDFTSEW 690
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BLAST of mRNA_Ecto-sp13_S_contig11426.1258.1 vs. uniprot
Match: A0A7S2PSB5_9STRA (Hypothetical protein n=2 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2PSB5_9STRA)

HSP 1 Score: 115 bits (289), Expect = 1.990e-25
Identity = 79/248 (31.85%), Postives = 126/248 (50.81%), Query Frame = 1 
Query:   94 AVAAYVTFDDEMAAVEARVSYSGSWI--TRLCV-KEKMKLKGKRISVEEAPEPSTILWENYGYRRCELWTRRTATLLVGTSFITLSIAMSIYAL-YQNQQ-AESEGGTAECPEGWDALSRREKEDAVAEDGTILHCLCDGGDGSAWTLSPNDVCYEYAWAKVQVTWLVLGTSFMVAFTNAAINKITRMMGVFEKHLSVDKQEVAVYRRLVLLKFVNIGLTVLVTNSRRVIDVLGVSL-DYEEDFTSEW 819
            A+ A+VTF  E   V+A  +Y  SWI  T  C    ++K +GK++ +++APEPSTILWEN  +       R+  T  V  S I +S+  +  A  +QN+  A+SE     CPE +      E+   V+ D ++LHC C   + S      +  CY+Y  + ++   +  G +  +   N     +    G FEKH SVD+ E ++  R+ LLKF+N G  VL+ N   +  ++ +   D + DF  +W
Sbjct:  759 AIQAFVTFRSEEGGVKAMKAYQLSWIRSTLFCFYPRRLKFRGKKLYIDKAPEPSTILWENLEHGYFNRMCRKGVTNTVALSAILVSVVFTFMARDFQNKTLAKSE---MPCPEDFTDAPENEQLSIVSSDESLLHCYCT--NLSILDQGRSSECYDYLKSNLESMAMSYGAAATICIMNVFFTWLMTKAGSFEKHHSVDRMEASIMFRVFLLKFINTGCLVLLFNQAWLQHMVNIEFEDKQPDFGVQW 1001
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BLAST of mRNA_Ecto-sp13_S_contig11426.1258.1 vs. uniprot
Match: K0SAW4_THAOC (PHM7_cyt domain-containing protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0SAW4_THAOC)

HSP 1 Score: 108 bits (270), Expect = 5.860e-23
Identity = 77/252 (30.56%), Postives = 119/252 (47.22%), Query Frame = 1 
Query:   91 KAVAAYVTFDDEMAAVEARVSYSGSWITR--LCVKEKMKLKGKRISVEEAPEPSTILWENYGYRRCELWTRRTATLLVGTSFITLSIAMSIYALYQNQQAESE---GGTAECPEGWDALSRREKEDAVAEDGTILHCLCDG-GDGSAWTLSPNDVCYEYAWAKVQVTWLVLGTSFMVAFTNAAINKITRMMGVFEKHLSVDKQEVAVYRRLVLLKFVNIGLTVLVTNSRRVIDVLGVSLDYEED---FTSEW 819
            +A+ A+VTF+ E   V+A  +Y+ SWI R   C   ++  KG R+ +++AP+PSTILWEN  Y   +   R+  T    TS   L+I  S+Y  +  +  + E     +  CP G+       +   V +D  + HC C        W+++    C+++     Q T        +V FTN     +    G FEKH S+D+ E +   R+ LLKFVN G  VL+     +  ++GV      D   F  EW
Sbjct:  318 RAIQAFVTFETETGFVKAMANYNLSWIRRNCCCYPTRLLFKGSRVDIDQAPQPSTILWENLEYTSGQRLVRKCLT----TSVALLAILFSVYITFLARDYKGEVLHKASRPCPSGFFEQDPAYQLKVVEQDLDLSHCYCRTLSIFKQWSINQ---CHDHLLTSSQATATSYAAGLIVVFTNIFFTILMDRAGRFEKHHSLDEMEASNMVRVFLLKFVNTGCIVLLYGQTWLQRLVGVQFSDASDLNTFGVEW 562
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BLAST of mRNA_Ecto-sp13_S_contig11426.1258.1 vs. uniprot
Match: F2E1A3_HORVV (Predicted protein n=1 Tax=Hordeum vulgare subsp. vulgare TaxID=112509 RepID=F2E1A3_HORVV)

HSP 1 Score: 57.8 bits (138), Expect = 8.560e-6
Identity = 53/234 (22.65%), Postives = 105/234 (44.87%), Query Frame = 1 
Query:   97 VAAYVTFDDEMAAVEARVSYSGS----WITRLC----VKEKMKLKGKRISVEEAPEPSTILWENYGYRRCELWTRRTATLLVGTSFITLSIAMSIYALYQNQQAESEGGTAECPEGWDALSRREKEDAVAEDGTI--LHCLC-DGGDGSAWTLSPND--VCYEYAWAKVQVTWLVLGTSFMVAFTNAAINKITRMMGVFEKHLSVDKQEVAVYRRLVLLKFVNIGLTVLVTNSR 759
            V+A+VTF    A  +   ++  +    +I  +C    V++K+  +GK +  + A +P +ILWEN G  +  ++ RR  +L +       +  + I + Y   + +    T +C +  D       +D       +  L C C D   G  + + PN+  +C  +   K+  T +     F+V   N  +  + + +  FEKH +++ Q      +    +F+N GL +L+ N++
Sbjct:  227 VSAFVTFSSIPARNKILDAFISTPFERFIRAICGGGNVQDKV-FEGKVLRAKSAADPGSILWENIGESKTNVYGRRAFSLALTVILWGTTAILLIVSTYYKNEFKKNFPTVDCGDYGDVTIDEAAKDYALGQYQVGKLECYCSDDLVGRLYDIFPNNQTLCVTWFNNKLIETSVTFAIVFIVLVINYGVQFVFQALSKFEKHSTLNNQLATRVIKTFAAQFLNTGLLLLLINAK 459
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BLAST of mRNA_Ecto-sp13_S_contig11426.1258.1 vs. uniprot
Match: A0A812WSS5_9DINO (Hypothetical protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A812WSS5_9DINO)

HSP 1 Score: 55.8 bits (133), Expect = 3.360e-5
Identity = 31/100 (31.00%), Postives = 54/100 (54.00%), Query Frame = 1 
Query:   94 AVAAYVTFDDEMAAVEARVSYSGS--WITRLCVKEKMKLKGKRISVEEAPEPSTILWENYGYRRCELWTRRTATLLVGTSFITLSIAMSIYALYQNQQAE 387
            AV A+VTF+ E + +     Y GS  ++ R+   + ++++G+ +SVE+AP PS +LWEN      + W R     ++    I  S  + +YA  +  +AE
Sbjct:  358 AVGAFVTFNHEESLIRCLDEYHGSNTFLGRMRQPKLLRMRGQPVSVEQAPNPSDVLWENLEVTDFQRWKRSFIIGIITLLLIAGSFGIVLYAKVERTKAE 457
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig11426.1258.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 5
Match NameE-valueIdentityDescription
D8LH14_ECTSI7.400e-17696.70PHM7_cyt domain-containing protein n=2 Tax=Ectocar... [more]
A0A7S2PSB5_9STRA1.990e-2531.85Hypothetical protein n=2 Tax=Leptocylindrus danicu... [more]
K0SAW4_THAOC5.860e-2330.56PHM7_cyt domain-containing protein n=1 Tax=Thalass... [more]
F2E1A3_HORVV8.560e-622.65Predicted protein n=1 Tax=Hordeum vulgare subsp. v... [more]
A0A812WSS5_9DINO3.360e-531.00Hypothetical protein n=1 Tax=Symbiodinium sp. KB8 ... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig11426contigEcto-sp13_S_contig11426:2151..5365 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score523.9
Seed ortholog evalue4.6e-146
Seed eggNOG ortholog2880.D8LH14
Model size824
Hectar predicted targeting categoryother localisation
Exons6
EggNOG OGs2CY1P@1,2S1C7@2759
Cds size822
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig11426.1258.1prot_Ecto-sp13_S_contig11426.1258.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig11426 2151..5365 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462599.0852177-CDS-Ecto-sp13_S_contig11426:2150..22971681462599.0852177-CDS-Ecto-sp13_S_contig11426:2150..2297Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11426 2151..2297 +
1681462599.0944417-CDS-Ecto-sp13_S_contig11426:2615..27461681462599.0944417-CDS-Ecto-sp13_S_contig11426:2615..2746Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11426 2616..2746 +
1681462599.1035416-CDS-Ecto-sp13_S_contig11426:3432..35471681462599.1035416-CDS-Ecto-sp13_S_contig11426:3432..3547Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11426 3433..3547 +
1681462599.1131735-CDS-Ecto-sp13_S_contig11426:4161..43231681462599.1131735-CDS-Ecto-sp13_S_contig11426:4161..4323Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11426 4162..4323 +
1681462599.1231737-CDS-Ecto-sp13_S_contig11426:4709..48381681462599.1231737-CDS-Ecto-sp13_S_contig11426:4709..4838Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11426 4710..4838 +
1681462599.1367884-CDS-Ecto-sp13_S_contig11426:5227..53651681462599.1367884-CDS-Ecto-sp13_S_contig11426:5227..5365Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig11426 5228..5365 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig11426.1258.1

>prot_Ecto-sp13_S_contig11426.1258.1 ID=prot_Ecto-sp13_S_contig11426.1258.1|Name=mRNA_Ecto-sp13_S_contig11426.1258.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=274bp
IRQLQEKRSKASRKIIHFAAEAKMAVMMTNKAVAAYVTFDDEMAAVEARV
SYSGSWITRLCVKEKMKLKGKRISVEEAPEPSTILWENYGYRRCELWTRR
TATLLVGTSFITLSIAMSIYALYQNQQAESEGGTAECPEGWDALSRREKE
DAVAEDGTILHCLCDGGDGSAWTLSPNDVCYEYAWAKVQVTWLVLGTSFM
VAFTNAAINKITRMMGVFEKHLSVDKQEVAVYRRLVLLKFVNIGLTVLVT
NSRRVIDVLGVSLDYEEDFTSEW*
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mRNA from alignment at Ecto-sp13_S_contig11426:2151..5365+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig11426.1258.1 ID=mRNA_Ecto-sp13_S_contig11426.1258.1|Name=mRNA_Ecto-sp13_S_contig11426.1258.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=3215bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11426:2151..5365+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATCCGCCAGCTCCAAGAGAAGCGATCTAAGGCAAGCCGGAAAATCATACA
CTTCGCGGCGGAGGCCAAGATGGCCGTCATGATGACCAACAAGGCAGTGG
CTGCCTATGTGACCTTCGACGACGAGATGGCGGCTGTAGAAGCCAGGGTA
AGAGACCAGACATTCTTGCCGTTTTTTCACGCTTTTTTGTCCAGCCATGG
CCTATTTAGCTTCATACACCCATGAGGTGGCTGGCGATTCTTGAAGCTGG
TAGGTAATATCTACTGTTTTGTGGTCATGTCGGAATGTTTTCGTTTGCGA
TATCTGTTTGGTTGAGTTTGTTACGATCGATGTGAATATCGCGTCTAAGT
CAGGGTAATGAGTATTGAGGGAAGCAGGGCGAGAGGGGCGGGAAATGAAT
GATGAGCAAGAACCCGCATCTCAAACCCGGTAGTGTTGTTTGGCTATTTT
GATGGCAACTGGAAGGTTTCCTACAGTGGCAGCTGGATCACGCGACTGTG
TGTCAAAGAGAAGATGAAGCTTAAGGGTAAGCGCATCAGCGTCGAGGAGG
CGCCCGAGCCGTCGACGATCTTGTGGGAAAACTACGGTTACCGGAGGTAC
GATGAGTCCCGGAAGCCAACTACTCGGTACCAATAGAACAAAACGTGCTA
AATCACGAAGCTGCTGCTATCGAAGCAATTCGACGCCAGATCGTTATGCT
GTAGCGCCATCCTTTCTTGTTTCCTTCGAATCGCTCGTCCTGGGTAAGGA
GCACCTTTGTCCGGTCCACAAGAGCAGCCAATCCCTAGACAAACCAGTTC
TCCATTGATCGACGTTGTTTAACGTGTTTCAAACCTTTGAAGAGATGACC
TCGAAAACTGTCGGCGGTTTCTTGTTGAAGTCTGCTGGTCTAGCAAACAA
GCAGAAGGCTCCAACATGGGGGTGGAGGGGGGGCATGTGGGGATGGCCTG
AAACATTCGTCTCATCTCCCCAACGTCTGTTGACGACGACTGATTCCCTC
TTTCAACACATTTCCATGTTTTCTTGTGATGTCTGATTGTTTTTCGTTTT
TTCCCGGTCTTGGTGTGAATCCAAGGTTATGGTTCGTCGGATCCACATGA
ACGTCTTGAACTGATCCATATCTTCGGGTACGAACGGTGGTTCACAAAAG
TGTTTGAAGAGACCTCGTGGTGATGTTTCCCTCTTCGCATCAACCAACCC
CTGCTAATAGGCAACAAACACAAATACGGCCGTCTAATTTCCGTTTCGGT
TATCACGTCATTGGACGTTGAATGTTCCGAAGGTGCGAGCTGTGGACGCG
GCGCACAGCGACCTTGCTCGTTGGGACGAGCTTTATCACCTTGTCCATCG
CCATGTCTATCTATGCCCTCTACCAGAACCAGCAGGCCGAGAGCGAGGTA
CGACGAGTGTAGTGATCCCCTTGTGAGGTGTTTGTACTGCACGTTTTGCT
ATCATCGTCTAAGGTGTTGCTCTTGTATTTTTTGTCGTTGTCCGCAACAT
GTACCATGCATGGTGGCCATGAACAAAGCATAAAGTCATACTAAATCCCC
CTTCCTCGTAAGCTGAACCTCTACAGCGCATGGCACACCGCCTGTGAGTA
CAGTAAGTGTGTCAAGCGCAGGGCGGCACCTGGCGTTTGGTGAAGATGGT
CCCTATCAGGTAGATTACCAGCGTCGAAAAGTTCATTCCGGGCCTCTGTG
CTCACAACCAGGCCCCAACTACGCCTGCCTTTCCTGCGAGCTACGCTGAC
AAGAACTTTGGCACATGGCTGGCAATGCCGAGTGGAAACCAAATACGAGT
ACGGTGTCACTACAGTACCTGGGTCGGCCCAACGAGACACACGGTCCCGG
AGCAAGACATTCGTTCATATACACTCCCCCCAACGAATTTGAAAAAGGTT
GGGGTGTTTCCGAGCACGATCCCCCCCCGCCAACAAACGACGACTCACGT
CTACAACTTGCGCTACTACCTCCTGCTGATAATACGTTGCCTGGTGTACT
GATGGCACAAGGGGGGCACCGCGGAGTGTCCCGAGGGTTGGGATGCACTG
TCTAGGCGGGAAAAAGAAGATGCTGTTGCGGAGGACGGAACTATCTTGCA
CTGCCTCTGCGACGGTGGCGATGGTTCGGCATGGACGCTCAGCCCCAATG
ACGTCTGCTACGAGTACGCCTGGGTAAGTTTGTTTCTCTCGTGCTCCTGT
CGACGTTGTTGTTGTTATCTCAGGTCGTGATGGTGGTTGTTGCTGTTGTC
TCCGGATGCTGATGGTGTTGTAACATTGGATTGCTGATGTAAGGACAAAG
ACACTACTGTTCTTAACGTTGGACGAATAGACTGGATCAGATGACCCGGC
AACATTGGCCATGATTTGTCTTCCAGTCGGAGAAACACTCAACATCTCAC
AGGTGCCGTGTTGCCGTACATAAATATAGCTGACAGACACTCCGCCTCAA
CTCCTTGCCTGTCGTTATCGCTATTCACACACGCAACAAGGTTCCACACC
GCAGTCTAGGCCCTTTCCTGGTACATCACTTCCCTTTCGATTTCGGTTGC
GTTTGGTAGGCCAAGGTCCAGGTAACCTGGCTTGTTCTGGGGACGTCGTT
CATGGTCGCCTTCACCAACGCCGCGATCAACAAAATAACTAGGATGATGG
GGGTCTTCGAGAAGCACCTGAGCGTGGACAAGCAAGAGGTAGGCGCCGAG
TAAGGAAATAACATAAAACACTCCCCTTCCTACATCCCTCGCGACAAAGT
TCGATCGGACTCCGAGGACCCTCGGCGTAAACGTCATGTCGTAGTTTTCT
TTGTGCTGTCGCTGCTGTTGCTGCTGCTTCGTGATGGTGATGATTTTGTT
TTTGTGCGATTCGCACGACCCCGGCAATCAACCAACCGACGGTTGGTGCT
ACCTAAGACTTGTTAACCGCCAAAATTACTTCGGAAATATGTATATGTTA
CTTGACTCATTTATTCTGCAACGTGCACATAAAGGGTTCGACACATGAAT
CAGCGGAGGTTTAGAGATAACAAAACCGGTGGGCTTGTACGGTGACTGTA
TTGTTACGCTGTGCATTCCCTTCGAAGGTGGCGGTTTACAGACGGCTGGT
GCTGCTCAAATTTGTCAACATCGGCCTGACTGTCTTGGTCACCAACAGCC
GCAGGGTCATCGATGTCTTAGGAGTTAGCCTCGACTACGAAGAAGATTTC
ACGTCCGAATGGTGA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig11426:2151..5365+

>mRNA_Ecto-sp13_S_contig11426.1258.1 ID=mRNA_Ecto-sp13_S_contig11426.1258.1|Name=mRNA_Ecto-sp13_S_contig11426.1258.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=822bp|location=Sequence derived from alignment at Ecto-sp13_S_contig11426:2151..5365+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATCCGCCAGCTCCAAGAGAAGCGATCTAAGGCAAGCCGGAAAATCATACA
CTTCGCGGCGGAGGCCAAGATGGCCGTCATGATGACCAACAAGGCAGTGG
CTGCCTATGTGACCTTCGACGACGAGATGGCGGCTGTAGAAGCCAGGGTT
TCCTACAGTGGCAGCTGGATCACGCGACTGTGTGTCAAAGAGAAGATGAA
GCTTAAGGGTAAGCGCATCAGCGTCGAGGAGGCGCCCGAGCCGTCGACGA
TCTTGTGGGAAAACTACGGTTACCGGAGGTGCGAGCTGTGGACGCGGCGC
ACAGCGACCTTGCTCGTTGGGACGAGCTTTATCACCTTGTCCATCGCCAT
GTCTATCTATGCCCTCTACCAGAACCAGCAGGCCGAGAGCGAGGGGGGCA
CCGCGGAGTGTCCCGAGGGTTGGGATGCACTGTCTAGGCGGGAAAAAGAA
GATGCTGTTGCGGAGGACGGAACTATCTTGCACTGCCTCTGCGACGGTGG
CGATGGTTCGGCATGGACGCTCAGCCCCAATGACGTCTGCTACGAGTACG
CCTGGGCCAAGGTCCAGGTAACCTGGCTTGTTCTGGGGACGTCGTTCATG
GTCGCCTTCACCAACGCCGCGATCAACAAAATAACTAGGATGATGGGGGT
CTTCGAGAAGCACCTGAGCGTGGACAAGCAAGAGGTGGCGGTTTACAGAC
GGCTGGTGCTGCTCAAATTTGTCAACATCGGCCTGACTGTCTTGGTCACC
AACAGCCGCAGGGTCATCGATGTCTTAGGAGTTAGCCTCGACTACGAAGA
AGATTTCACGTCCGAATGGTGA
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