mRNA_Ecto-sp13_S_contig113.1152.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig113.1152.1
Unique NamemRNA_Ecto-sp13_S_contig113.1152.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig113.1152.1 vs. uniprot
Match: D7FXJ8_ECTSI (Pinin_SDK_memA domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FXJ8_ECTSI)

HSP 1 Score: 979 bits (2532), Expect = 0.000e+0
Identity = 695/799 (86.98%), Postives = 702/799 (87.86%), Query Frame = 2 
Query:  143 MDFDRNAIGSLEDVHREWDSLETERAKVEDKLRSFDAAAGGRGGGFGGRGVRVIGGGRGRDRSGGGDWRRDRPRDTSNGGREEAPHRPSQVSRDKYRAMSGTWERGLDPATAALDDDAPPEGRERGGGGTAGLRGGTKGSIAHYQRPADSHGDRRGRGRDRGVAPMHGAGVRGDRDGMRGDRTGDRDVFARGGXXXXXXXTARXDFXXXXXXXXXXXXXXXRARRESNGXXXXXXEPEAKRPRVTSTIVVAGDEDTIGDGTMENGELDRWNTELPPASXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEVTGQEGDQQPMEEDDGCGRRPVGKIVVVSGGGPRGRAANIGGVAGSGGGRGSLASRLGPXXXXXXXXXXXXXXXXXXXXFVPLSERPMPEPSKPELHVAYKDGDTKKRNRRMFGGLMAHLGRARQQLDHDKSIIQRQKSVVEMAAIRQAQEKRQRRQTAENAAREERDEELHKRDYIRARQQKTLVALHGNGFIANQEQLKGFIFTKTLPKLAWSPNEHTDVTQALLADSSKEIDGNIERRKEADDKEFKEIDDSVAERAATRASRRMAHAPGVRQMGVRQDMDANPHGEGNGHRWQMAAGESDTNGEGPGQAHTVAQDDDDGNAGATEDIGGAPSMSQDVVDDGDQPGVAVSEADGGEGREMAGGAGDGMEVDGDDVGVATGLAEADDEEGQEGAEASPPRSLAPAAAVGGDLSTGEXXXXXXXXXXXXXXXXXXXXXXXXXXXR-VEGRTEAIVEAIGEEEVSRA 2536
            MDFDRNAIGSLEDVHREWDSLETER +VEDKLRSFDA AGGRGGGFGGRGVRV+GGGRGRDRS GGDWRRDRPRDTSNGGREEAPHRPSQ SRDKYRAMSGTWERGLDPATAALDDDAPPEGR RGGGGTAGLRGGTKGSIAHYQRPADSHGDRRGRGRDRGVAPMHGAGVRGDRDGMRGDRTGDRDVF+R  XXXXXXX   X  XXXXXXXXXXXXXXX        XXXXXX        VTSTIVVAGDEDT GDGT ENGELDRWNTELPPASXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX                                GGPRGRAANIGGVAGSGGGRGSLASRLGPXXXXXXXXXXXXXXXXXXXXF+PLSERPMPEPSKPELHVAYKDGDTKKRNRRMFGGLMAHLGRARQQLDHDKSIIQRQKSVVEMAAIRQAQEKRQRRQ AE AAREERDEELHKRDYIRARQQKTLVALHGNGFIANQEQLKGFIFTKTLPKLAWSPNEHTDVTQALLADSSKEIDGNIERRKEADDKEF++IDDSVAERAATRASRRMAHAPG RQMGVRQD DAN HGEGNGHRWQMAAGESDTNGEGPGQA TVAQDD DGNAGAT D GGA S SQDVVDD DQPG AVSEADG EGREMAGGAGDGMEVDGDDVGVATG AEADD  GQEGAE SP RSLAPAA          XXXXXXXXXXXXXXXXXXXXXXXX     V+GRTEAI EAIGEEEVSRA
Sbjct:    1 MDFDRNAIGSLEDVHREWDSLETERVRVEDKLRSFDAPAGGRGGGFGGRGVRVMGGGRGRDRSDGGDWRRDRPRDTSNGGREEAPHRPSQASRDKYRAMSGTWERGLDPATAALDDDAPPEGRGRGGGGTAGLRGGTKGSIAHYQRPADSHGDRRGRGRDRGVAPMHGAGVRGDRDGMRGDRTGDRDVFSRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVTSTIVVAGDEDTNGDGTTENGELDRWNTELPPASXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGGPRGRAANIGGVAGSGGGRGSLASRLGPXXXXXXXXXXXXXXXXXXXXFMPLSERPMPEPSKPELHVAYKDGDTKKRNRRMFGGLMAHLGRARQQLDHDKSIIQRQKSVVEMAAIRQAQEKRQRRQMAETAAREERDEELHKRDYIRARQQKTLVALHGNGFIANQEQLKGFIFTKTLPKLAWSPNEHTDVTQALLADSSKEIDGNIERRKEADDKEFQKIDDSVAERAATRASRRMAHAPGGRQMGVRQDRDANSHGEGNGHRWQMAAGESDTNGEGPGQARTVAQDDGDGNAGATGDTGGASSRSQDVVDDADQPGAAVSEADGAEGREMAGGAGDGMEVDGDDVGVATGYAEADDAGGQEGAETSPRRSLAPAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDGGDGVDGRTEAIAEAIGEEEVSRA 799
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BLAST of mRNA_Ecto-sp13_S_contig113.1152.1 vs. uniprot
Match: A0A7S2R5E1_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2R5E1_9STRA)

HSP 1 Score: 102 bits (253), Expect = 9.750e-19
Identity = 66/174 (37.93%), Postives = 96/174 (55.17%), Query Frame = 2 
Query: 1415 KPELHVAYKDGDTKKRNRRMFGGLMAHLGRARQQLDHDKSIIQRQKSVVEMAAIRQAQEKRQRRQTAENAAREERDEELHKRDYIRARQQKTLVALHGNGFIANQEQLKGFIFTKTLPKLAWSPNEHTDVTQALLADSSKEIDGNIERRKEADDKEFKEIDDSVAERAATRASR 1936
            KPE   AY+D   KKRNRRMFG LM HLG A++ L  D+  IQ +  V E   ++  +  ++ R+     +  E+++ L +RD I    +KT  AL    +   Q QL+ FI TK  P + W P +H D T ALLA+S   ++  I  R+  D++EF  I+  + ERA  R +R
Sbjct:  183 KPESQEAYEDEGVKKRNRRMFGALMGHLGTAQRLLKRDEKKIQSKAKVHETVELKNKESSQKLRKLEREMSAAEKEKALTRRDEILVEMRKTERALMTADWEKRQRQLEPFIATKAQPAIYWLPKDHNDATTALLAESKAAVERLITDRRAEDEQEFVTIETQIRERAEKRQAR 356
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BLAST of mRNA_Ecto-sp13_S_contig113.1152.1 vs. uniprot
Match: A0A835ZEL7_9STRA (Pinin_SDK_memA domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZEL7_9STRA)

HSP 1 Score: 84.3 bits (207), Expect = 6.860e-13
Identity = 51/150 (34.00%), Postives = 82/150 (54.67%), Query Frame = 2 
Query: 1457 KRNRRMFGGLMAHLGRARQQLDHDKSIIQRQKSVVEMAAIRQAQEKRQRRQTAENAAREERDEELHKRDYIRARQQKTLVALHGNGFIANQEQLKGFIFTKTLPKLAWSPNEHTDVTQALLADSSKEIDGNIERRKEADDKEFKEIDDSV 1906
            KR++RMFG LM HLG ARQ+LD DK++I  Q  +    A +Q Q+ +Q RQ      ++ R+ ++ +R     R Q          ++  QE L+G + T+ LP L W PNEH D+TQA+       +D  ++ R + D+   + I++ +
Sbjct:  161 KRDKRMFGALMGHLGAARQRLDRDKAVIDTQARLARSVADKQQQQSQQLRQLERELEQQRRNLDMARRAEAAGRVQVEERVAVTQLWLKAQEPLRGVLVTRALPPLTWLPNEHNDITQAMAQAQRAAVDDAMQARLDEDEDFARSIEEDI 310
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BLAST of mRNA_Ecto-sp13_S_contig113.1152.1 vs. uniprot
Match: A0A7S2BBZ9_9STRA (Hypothetical protein (Fragment) n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2BBZ9_9STRA)

HSP 1 Score: 68.9 bits (167), Expect = 1.370e-9
Identity = 43/119 (36.13%), Postives = 66/119 (55.46%), Query Frame = 2 
Query: 1433 AYKDGDTKKRNRRMFGGLMAHLGRARQQLDHDKSIIQRQKSVVEMAAIRQAQEKRQRRQTAENAAREERDEELHKRDYIRARQQKTLVALHGNGFIANQEQLKGFIFTKTLPKLAWSPN 1789
            AY     KKRNRRMFG LM HLG AR  +  D  +I+++  V +    + A+   + R+     +  E+++   KRD I  + ++T +++    +I  Q++LK FI TKT P L W PN
Sbjct:   46 AYSGDAVKKRNRRMFGALMGHLGNARSAIKRDSKLIEQKAKVEQTVRNKNAESSVRIRKLERQMSISEKEKAFTKRDQILCQMRQTELSMLTEEWIQRQQKLKQFIGTKTEPVLFWMPN 164
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BLAST of mRNA_Ecto-sp13_S_contig113.1152.1 vs. uniprot
Match: A0A7S0GT65_9EUKA (Hypothetical protein n=1 Tax=Amorphochlora amoebiformis TaxID=1561963 RepID=A0A7S0GT65_9EUKA)

HSP 1 Score: 67.4 bits (163), Expect = 8.450e-8
Identity = 47/135 (34.81%), Postives = 75/135 (55.56%), Query Frame = 2 
Query: 1457 KRNRRMFGGLMAHLGRARQQLDHDKSIIQ--RQKSVVEMAAIRQAQEKRQRRQTAENAAREERDEELHKRDYIRARQQKTLVALHGNGFIANQEQLKGFIFTKTLPKLAWSPNEHTDVTQALLADSSKEIDGNIE 1855
            KR+RR+FG LM HLG+A+ QLD  +   Q  ++KS+ E    R  + + +  + A+  A +++ + L  RD I   Q+K  + L       ++++L  FI TKT P L W P +H + T+ LLA   +E +  IE
Sbjct:  179 KRDRRLFGMLMGHLGKAKSQLDRLRKSKQARKKKSMEEKVEARMVEVRHEAHEHAKRRAEKKKMKVLKNRDEILREQRKKHMLLLQLRLADHEKKLSNFIRTKTTPGLCWLPGKHNESTEKLLAKGREESEKRIE 313
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BLAST of mRNA_Ecto-sp13_S_contig113.1152.1 vs. uniprot
Match: A0A4D9CWH8_9STRA (Pinin_SDK_memA domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CWH8_9STRA)

HSP 1 Score: 65.5 bits (158), Expect = 4.050e-7
Identity = 53/177 (29.94%), Postives = 92/177 (51.98%), Query Frame = 2 
Query: 1400 MPEPSKPELHVAYKDGDTKKRNRRMFGGLMAHLGRARQQLDHD---KSIIQRQKSVVEMAAI--RQAQEKRQRRQTAENAAREERDEELHKRDYIRARQQKTLVALHGNGFIANQEQLKGFIFTKTLPKLAWSPNEHTDVTQALLADSSKEIDGNIERRKEADDKEFKEIDDSVAER 1915
            +PE  K E+   YK  + +KR+RRMFG LM HLG A+ +L  D     I+++Q+ V+   +   R+  EKR+ ++  E AA   R  E  +R ++  RQ+     L    +        GFI T T P+L + P   T+  + L+  +  E++  + R++E  D+  +E++ +  E+
Sbjct:  173 LPEAEKGEVKEIYKTDEVQKRDRRMFGALMGHLGAAQARLRQDTGASGILKKQREVLTNVSEKDREMAEKRRAQKAEEQAA--ARRGEGKERAWLEVRQKTVEKELDCARYRDYWRAFSGFILTDTEPRLFYVPARMTEALEKLVEKTRGEMEAKVGRKEEEKDESVRELERAFREK 347
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig113.1152.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
D7FXJ8_ECTSI0.000e+086.98Pinin_SDK_memA domain-containing protein n=2 Tax=E... [more]
A0A7S2R5E1_9STRA9.750e-1937.93Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A835ZEL7_9STRA6.860e-1334.00Pinin_SDK_memA domain-containing protein n=1 Tax=T... [more]
A0A7S2BBZ9_9STRA1.370e-936.13Hypothetical protein (Fragment) n=1 Tax=Florenciel... [more]
A0A7S0GT65_9EUKA8.450e-834.81Hypothetical protein n=1 Tax=Amorphochlora amoebif... [more]
A0A4D9CWH8_9STRA4.050e-729.94Pinin_SDK_memA domain-containing protein n=1 Tax=N... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig113contigEcto-sp13_S_contig113:330..9364 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score62.8
Seed ortholog evalue9e-07
Seed eggNOG ortholog227086.JGI_V11_73139
Model size3909
KEGG koko:K13114
KEGG Pathwayko03013,ko03015,map03013,map03015
KEGG ModuleM00430
Hectar predicted targeting categoryother localisation
GOsGO:0000375,GO:0000377,GO:0000398,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005681,GO:0005911,GO:0006139,GO:0006396,GO:0006397,GO:0006725,GO:0006807,GO:0007155,GO:0008150,GO:0008152,GO:0008380,GO:0009987,GO:0010467,GO:0016070,GO:0016071,GO:0022610,GO:0030054,GO:0032991,GO:0034641,GO:0035145,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044464,GO:0045292,GO:0046483,GO:0071013,GO:0071704,GO:0090304,GO:1901360,GO:1902494,GO:1990904
Exons9
EggNOG free text desc.RNA splicing
EggNOG OGsKOG3756@1,KOG3756@2759
Ec32 ortholog descriptionPinin/SDK/MemA protein
Ec32 orthologEc-28_001970.1
Cds size2517
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko03019,ko03041
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig113.1152.1prot_Ecto-sp13_S_contig113.1152.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig113 472..8114 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462590.8070672-UTR-Ecto-sp13_S_contig113:329..4711681462590.8070672-UTR-Ecto-sp13_S_contig113:329..471Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig113 330..471 +
1681462590.9026098-UTR-Ecto-sp13_S_contig113:8114..93641681462590.9026098-UTR-Ecto-sp13_S_contig113:8114..9364Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig113 8115..9364 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462590.8199391-CDS-Ecto-sp13_S_contig113:471..6861681462590.8199391-CDS-Ecto-sp13_S_contig113:471..686Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig113 472..686 +
1681462590.8303413-CDS-Ecto-sp13_S_contig113:1950..30631681462590.8303413-CDS-Ecto-sp13_S_contig113:1950..3063Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig113 1951..3063 +
1681462590.8396094-CDS-Ecto-sp13_S_contig113:3492..35531681462590.8396094-CDS-Ecto-sp13_S_contig113:3492..3553Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig113 3493..3553 +
1681462590.8492458-CDS-Ecto-sp13_S_contig113:3825..39271681462590.8492458-CDS-Ecto-sp13_S_contig113:3825..3927Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig113 3826..3927 +
1681462590.860676-CDS-Ecto-sp13_S_contig113:4671..47971681462590.860676-CDS-Ecto-sp13_S_contig113:4671..4797Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig113 4672..4797 +
1681462590.8688831-CDS-Ecto-sp13_S_contig113:5287..53381681462590.8688831-CDS-Ecto-sp13_S_contig113:5287..5338Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig113 5288..5338 +
1681462590.8772502-CDS-Ecto-sp13_S_contig113:5809..59021681462590.8772502-CDS-Ecto-sp13_S_contig113:5809..5902Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig113 5810..5902 +
1681462590.8852115-CDS-Ecto-sp13_S_contig113:6620..67611681462590.8852115-CDS-Ecto-sp13_S_contig113:6620..6761Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig113 6621..6761 +
1681462590.8947868-CDS-Ecto-sp13_S_contig113:7499..81141681462590.8947868-CDS-Ecto-sp13_S_contig113:7499..8114Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig113 7500..8114 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig113.1152.1

>prot_Ecto-sp13_S_contig113.1152.1 ID=prot_Ecto-sp13_S_contig113.1152.1|Name=mRNA_Ecto-sp13_S_contig113.1152.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=839bp
MDFDRNAIGSLEDVHREWDSLETERAKVEDKLRSFDAAAGGRGGGFGGRG
VRVIGGGRGRDRSGGGDWRRDRPRDTSNGGREEAPHRPSQVSRDKYRAMS
GTWERGLDPATAALDDDAPPEGRERGGGGTAGLRGGTKGSIAHYQRPADS
HGDRRGRGRDRGVAPMHGAGVRGDRDGMRGDRTGDRDVFARGGRGGTRGD
TARGDFDRFRNGGSGGRGGGSRARRESNGVGSGGGEPEAKRPRVTSTIVV
AGDEDTIGDGTMENGELDRWNTELPPASPPPAAEAAVATDIAATTTTTPD
DDLVDYEPEAEPEEDTASGPPPAAAAAAPPGEVTGQEGDQQPMEEDDGCG
RRPVGKIVVVSGGGPRGRAANIGGVAGSGGGRGSLASRLGPPMGRPVMVK
GGSGDNGGEGGFVPLSERPMPEPSKPELHVAYKDGDTKKRNRRMFGGLMA
HLGRARQQLDHDKSIIQRQKSVVEMAAIRQAQEKRQRRQTAENAAREERD
EELHKRDYIRARQQKTLVALHGNGFIANQEQLKGFIFTKTLPKLAWSPNE
HTDVTQALLADSSKEIDGNIERRKEADDKEFKEIDDSVAERAATRASRRM
AHAPGVRQMGVRQDMDANPHGEGNGHRWQMAAGESDTNGEGPGQAHTVAQ
DDDDGNAGATEDIGGAPSMSQDVVDDGDQPGVAVSEADGGEGREMAGGAG
DGMEVDGDDVGVATGLAEADDEEGQEGAEASPPRSLAPAAAVGGDLSTGE
EENNGGCRGGGDVEKVGGGGGGSDGGGRVEGRTEAIVEAIGEEEVSRAGG
GGDTDADEETKKKKKSSKKDRGSSSSGRKKSSRRSSDK*
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mRNA from alignment at Ecto-sp13_S_contig113:330..9364+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig113.1152.1 ID=mRNA_Ecto-sp13_S_contig113.1152.1|Name=mRNA_Ecto-sp13_S_contig113.1152.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=9035bp|location=Sequence derived from alignment at Ecto-sp13_S_contig113:330..9364+ (Ectocarpus species13 EcNAP12_S_4_19m)
GCCCGACTTATGGGGCCCAACTCTTGAGTGTCTTACCACCACCGCTGCTG
ATCGCAGAAGGACGGTGATTGCCCACTTGTCGCCTTGACGTGCTGCTGTT
AGATATAATCACGTATACCCGTATCTGGCTCATTTCAGCGTGATGGATTT
CGACCGGAATGCCATCGGATCTCTGGAGGACGTCCATCGGGAATGGGACA
GCCTCGAGACCGAGCGTGCCAAGGTGGAAGACAAGCTGCGGAGTTTCGAC
GCAGCGGCTGGGGGTAGGGGCGGTGGCTTTGGCGGGCGGGGGGTAAGGGT
TATTGGCGGCGGCAGGGGTAGGGACAGGAGTGGCGGGGGAGACTGGCGCA
GGGATAGGTGAGAGCACGACGGCAGCAGCACGGGTTCTACAGCTGTACGT
TGATGGACGTCGGTGTTGGTACCGGACGATGAACCAGATCCTACCCGCAC
GGGATGCAACACTCACACGTGCCTACTAGGAGCATGTGGCATGCTGTAAG
GCGGCACGTCGAAGCATCTTCGCGACCCTGGCCATGAAAACTAATGAGAG
GGCGGGCGAAACCTTATGTGTTCTTGGCGTCGATATTGATCCAAGATAAC
CACACACCCTTGTACAACGATCATCGCGATTGCCAAGCGGACATCCCTTT
GCATCCCAAGCGAAAGCTTCGCTTGCATGCTGACTCCGTACGTATTCCGA
AGCCTTAGTTATGCTCGGGGCTGCCCACTGCGCAGAGAGTCCGGGCGGTG
AGCTGACAGCAGTTCCCGAACTGCTGGTGCGTGGTTTCTGAAATCCGGTG
TCAGCCCAAATTGTTCGGACAAAGTGGTGCAATTGCCGGCTAGGATATAC
GAGTAAGACACGACGTGACAAGCCGAAATCTGCACAGGCCTGGAGATAAG
AAACATCAAATCGGGTCGAGGCTCAGTTTCGTCGTCACTGCTGTCGAGCG
CAGGTTGTTTCATAGAGTCTAGAAATTCGGCTGATGTGTGTTTCTGAACC
TTACCTTCTTGTTATAATGCGAGCATCTGTTTCTGAACAAAATATTTCTT
GATTGCGGGATCGTACTGCTGCATCTGTGTACGGACATGGGTCATGATCA
CTCTTACATGCACACGCACCACCGCCTCCTTAGCGGCAACGTTACACCTG
TGCGTTTGTAGTCGAACCGGGGTCAGGGTGAGTCGGGACAGGCAGGGATG
AATGTGTGCCGTGCACGCCCATCCCAGAGGACGGTTTGGTGCGGCTCTTG
CACGGAACGTGGCTCGTGTTGGTGTTGCTACCGGGTTGCCCTTACCCATC
TTGGGAACGAGTATGGTCGCTAAGATGCCGAGAGGTCGGCGGGAAAGTTC
GAGGGAAAATTCTGACGGTGGAAAACCAACGCCGCGGCCCGTGGCCCGTT
TCACGTTCCCCCGCATCCGGCCAGGAGGTCGTATTTTCTCCGCTCTCAGC
GCCCTGCTTCCCACCATCCCCCTACACGCGCACAGATGATCACCACCCTA
CACACCTGGTACGTGCGGCTGTGGTAAAGTTTCCCCGGCACACAAAAATG
TCGAACAAACGCACACATTGCGATGACTCTTATGCTCTTACCCCTGTCTT
TGTTCTTAAACTGTAACGCAGGCCCCGCGACACCAGCAATGGCGGTCGCG
AGGAGGCCCCGCACAGACCTTCCCAAGTATCGCGAGACAAGTACCGCGCG
ATGTCGGGTACGTGGGAGCGAGGGCTAGACCCTGCAACGGCTGCCCTGGA
CGATGACGCCCCGCCGGAAGGCAGGGAGAGGGGCGGCGGCGGGACAGCGG
GGCTTCGGGGAGGCACCAAGGGTTCCATCGCGCACTACCAACGCCCCGCA
GACAGCCACGGTGACAGGAGGGGACGGGGTAGAGACCGCGGCGTTGCTCC
AATGCATGGAGCTGGTGTGCGGGGAGACAGGGACGGTATGCGGGGCGATA
GGACCGGCGACAGGGACGTCTTCGCGCGGGGAGGACGAGGCGGAACGCGG
GGTGATACGGCGCGGGGAGACTTCGATCGGTTCAGGAACGGTGGCTCGGG
GGGACGAGGAGGAGGGTCGCGGGCACGCCGCGAGTCCAACGGCGTCGGGA
GTGGCGGCGGTGAGCCGGAAGCGAAGCGACCTCGGGTGACGAGCACGATC
GTCGTCGCGGGTGATGAGGACACCATCGGGGACGGGACGATGGAAAACGG
CGAGCTGGACCGTTGGAACACGGAGCTTCCCCCCGCATCGCCACCACCAG
CGGCAGAAGCTGCTGTCGCAACAGACATAGCAGCGACGACAACAACAACT
CCAGACGACGACCTCGTGGACTACGAGCCGGAGGCAGAACCGGAGGAGGA
CACCGCTAGCGGGCCTCCACCCGCCGCTGCCGCCGCCGCCCCCCCCGGCG
AGGTAACGGGCCAGGAAGGCGATCAGCAGCCCATGGAAGAGGACGACGGC
TGCGGGCGTAGGCCCGTCGGCAAGATCGTGGTCGTTTCGGGCGGCGGCCC
TAGAGGGCGCGCTGCCAACATCGGCGGCGTCGCCGGCAGCGGCGGCGGGC
GCGGGAGCCTGGCTAGCAGGTTGGGCCCTCCCATGGGGAGGCCGGTGATG
GTGAAAGGGGGTTCGGGGGACAACGGCGGGGAAGGGGGCTTTGTGCCTCT
GTCGGAGAGACCCATGCCGGAGCCCTCGAAGCCAGAGCTTCACGTGGCGT
ACAAGGACGGGGACACCAAGAAGCGAAACAGGAGGTGCTCCATATACCAT
AGAAATTCGCCCGCCGTTTTGCTGTTTGTTTTTGCCGGCGTCGCTGTTTC
GTTTTTTTTTTTGTTTCAAGAGCAGCGATTCAAGTGCTGCCTCATCCCCC
GAACACCCCACACCCCGCTTGGCCTCGTTGATACGCCTTCGAGGCTTACT
TTTTCAGGTTTGCTCCCTCGCGCTGTGGCCACGAGTGTCCCCTCACGGCA
TCCTTTCTCCCTCCGCCATGTGTGTCGCGCCCCCCCCCCCCCCCCCCCCC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCGC
CCCCCCCGCCCCCCCCACACCTACCTTCCCCCCTCCCCCAAGGGCGGGGC
GCCCCCCCCCCCCCCCCCGTCTCACTTCCCGATGCGCCGTCTGCACCACC
ACCCCCATCTCAGGATGTTCGGTGGCCTCATGGCCCACCTCGGCCGAGCA
CGACAGCAGCTAGACCATGACAAGGTATGCCGCGTGATAGCGCCAGGTGC
ACAACGGAAGGGAAAATGACGGATTTTGATAAACATGCGGTAGCGCCGTC
GTTGCTACTGCTGTTGCCAGTATCGCGCATTTCCTCCCTGCTCCGACCTC
TCTGCTCCCTTTGGCATATTTTTTTCGTGCGTATTTTCCGATGGCGTACC
CGGCGTTACCGTTCCTTTTTCCGGTCTGTCTGATTTTGGGCGCGCGCGCG
TCGGGGGGGGGGGCGCCGTGGCATGACAACGGTCGAACGTGCACAGTCCA
TTATACAGCGGCAGAAGAGCGTTGTCGAGATGGCGGCGATCAGGCAGGCG
CAAGAGAAGAGGCAACGCCGACAGACGGCTGAAAATGCGGCTCGTGAGGT
GAGAGACCGGACGGGACAGGATGGCATGGAATGGCGATAGAATGGGATAG
GATGGGAAAGGATGAGATAGAATGGGATAGGATGAGACGGAACGAGGCTT
CTCGGTGTCATTTTATCGCAAGTACAGGGTCACGTTCCGCCCCGGAGGAA
TGTTCACCACTTTCCTTCTACAGTATTCAATACCTAATGGTAGCTCCTCT
TCTTCGTGCGTGAGAGCGTGGATGTGTGCAGCTGGCAATTGGAATGGACC
CCACCACGTGAAGGAGCGAAGAGCATCGCGTTTGTGTCGACGCGGTTGGT
GTTCCCCCCGCTGCTATGTGGCCCCGCTGTGACTCGAATTTGAAGTTTCA
CGTCGAGTGTCACCTTGCTCGGTGCTTTATTTTTGTCGCCCTTTATTGTC
GCGGCCCATTCCACCCCTGACGGGGGTCCCAATGCCGAAGAAAGGAGCTC
GTAGGTATTCAATAGGGGGAGAGTGGTGGGACATTTTCTTGGGGCGGGAC
GTGTCCCGATGTGTGCGACACTAGATCACGAGTTCTCAAGGAACAGCTTT
TGAGCCACCTGGGTCTGCAATTTTTTGCCTGTACATGTTCTCGATGCCCA
GAGAAACATTGTTGGTCATCATCCTGTACATGTTCTTTTTTTACTGGGAG
AAATGTGCCTGGCATCTGTCCTGCTTCTGAGTGTGCTCCTCCTTCGATTG
TGTGTGTGTGTGCGCGTGCGTGTGCTTGCGTCGAATCGGCAGGAGCGCGA
TGAGGAGCTGCACAAGCGCGACTACATCCGCGCGAGGCAGCAGAAGACCT
TGGTTGCTCTTCACGGAAACGGTTTCATCGCCAACCAGGAGCAGCTCAAG
GGGTTCATCTTCACAAAGGTATGCCTCACGACAGCGCCAGCAACATTGTA
GTACGGAAGCGTGCAGAGACTACCATGATTTGGCTTGAGGCAACCCGGCA
GCACGTTGACGGGAACACCGTAGGTAACCTTACGCAAGATTTCAGCCACC
CTGAACCTATTTCCAACGCTCGATTGCTCCACGCCAAAGAAGATGCCACC
GCTGAAAAAGTGCGCATTTTCGGCAAGCTGTCGACGGTAGATGTTTGCAG
CGTCACCATTTTCGGCACCGCCACTCTTTCTTCTGACGAGACCGCGACCT
TCGAAAATTGGTCAGGGTGTGTAGTCATCTTGTCACCTACTACGGTACCT
AGTGCCAATTGACTTGCCGTTGCCGTGTAGATCTCCACGTGTGCAGGCCG
AGCATTCATCACCGTGTTTCAACCCATACGCTGTACGCGCACGCCCCAGG
TCTGCTGCTCATCCTCCTCCCGCCTTGCCCGCCGTTTCCAATTTGTCGAC
TTTCGCAGACCCTGCCCAAGCTAGCTTGGTCACCCAACGAGCATACCGAC
GTTACCCAGGTGTGCCTTGTCGCGATTGGTTGCTGTTATTGACGCCGGTG
CCCCCGTCCTCCCCCCACCCGGTCGCGTCAATTTTTTTGTTTCTGTTTTG
CTTGGTTGTCCTGCTTATGGGGCTGGTGTAGACGAACAACGTGGGCCTTG
CCATTCTCTGTTTGTGTGCTTGATTTTTTGGGGGTAGCGTAGCTTTACTT
ATGGTAGCAACACCGAAGCGATTGAGCCAATCAAAACGGCCGATTCGTTT
GGTATTTCAGCACTTGATGCTTTTCGGAAAATCGAACCCTGTGTCTTTAA
TCCTTGTTTTTTTTTTACATCTATCTCGTGATGAGTATCCCGCCCTTTTA
GGAAGCTGCGCATGCCTGGCTGCGCATTGCTGCCTCTCTTTATGGTACCT
ATGTTGCCGTTTGCCGCTTTTTAACCGTTGCTGTGACGTTCTGTTTGCTC
CCTCCCATTTTCTCCCTCTCTCTTGCGCAGGCTCTGCTGGCAGACTCTAG
CAAGGAGATTGACGGTAACATCGAACGGAGGAAAGAGGCCGACGACAAGG
AGTTCAAGGAGATCGACGATTCGGTGAGTGGATCCCCCCCCCTCTCTGTC
TGTCTGTCTCTCTCCCTCTCCCTCTCTCCCGCTCTCCCTCTGCCTCTCTC
TCTCTCTCTCTCTCGCTCTCTTTCTCTGTCTCTCTGCCTCTGTCTCTCTA
TTTCTCTCTCTCCCTCACTGTCTGGCTGTCTCTGTCTCTGTCTCTGTCTC
TCTGTCTCCCTGTCTCTTTCCCTCCTTGTTCTGGAAAGTGCCGATTCTGT
CATGTGTGACGGATTCTTCCGAACAACAAGAAATATGTGGCGCTGACGAA
GTTCCACATTTTTTCGCAAAGGACACCTTCTTGTATTACTGCGTATACCA
GCCTGCTCCCCCTTCTAAACGGCAGAGAGGTCCTCCTCGAGCAGCTTCTT
CCTTACGCAACATGCCTACCAGCTCCCTCTCGCCGCCGCCCCGTCGTGGC
ACACGTATCACCTGCACGTAGGTTTCGATTCCACCTTTAGCCTTTCAACC
GCCTTTGGTCGCGGCGGCCACGCATGGATGGTGTACCTTTTTCTGACACC
GCTTGCGTTCTTGAAGCATTTCGTGCGGACTGAAAACGACCCGCCGTCGC
ACACAACTCCTAATCGAAAGGTATGCGGAGGTCGCAGGGTCCAATGGCTC
GGCAACCCCTCCCCCCCATGTGCCCAACGCCCCCCCCCCCCCCCCCCACC
CCCCCACCCCCATCTCTGCCGCCACCCTGCCGCCCCATCAGGTTGCAGAA
CGAGCCGCGACGCGGGCCAGCCGGAGGATGGCACACGCTCCGGGGGTGCG
GCAGATGGGGGTGCGGCAAGACATGGACGCTAACCCTCACGGAGAAGGCA
ACGGGCATCGCTGGCAGATGGCGGCGGGAGAGGTGAGCTTTTGCGCGGTC
GGGCTGAAAAGTGTTCGAGGAACTACTTTAATTGCCACCGTGTGTCTTTT
AGTCGCGTGTTTTTTTTTGTGTTGTACTTCTTTCCAGGAGTGATATCATA
CATCAGTGCACTCTTGAGGTTGGCGAGGAGCAGTTTCGTGCTTCGCATAT
GCTTTTTTGACGACGAAGAAAAGCGACATAAGAACGACGGTCGTTCTTGA
AGGGGGCTTGCGTTTGTGTGGCCTCTAGCCAGACTTCGTTTGAGTACAGA
CTATGGTAGATCAGGATAAAACCATGGGCACAGGGAAGCGCAGAGGTCTG
GAAGCCAGAGAAATGTCGCGGAAGTTTCTCGTGAGCGGAAGCTTGAAAAT
CGGCGGCGAGTATCGGATGGACCCATGACGAGCAGATTCAGATAACAGTG
CGCTGCGTAATAGGCGGGGCAACATGCAAGTTGTGCCCACCTTTTTCGTC
TCAACACCTGCACCTTTTATTGATGATGATGATGGTCTCGCGAGGGACGG
AACCGGCGCTGCGCCATCTGTGCTCGGGGATAGGATGATCTCCAATCCCT
TGACATTGAATCTTATGTTGCCATCAGTATGTGTCTTAGTGTCGTCCTTG
GTGGTGTGTGCCACCGTACTGGCGTTCGGCGATTCTCTCTCCCCCCTTCT
GCCTCTCGAGCGCCATCCCCTTCCACGATTTGTGCCGATCTGTTCTCCGC
ACGCACCCTTAACCCTCCAGAGTGACACAAATGGGGAGGGTCCCGGCCAA
GCCCACACGGTCGCTCAGGATGATGACGACGGCAATGCTGGTGCGACAGA
GGACATTGGCGGCGCCCCGTCCATGAGCCAAGACGTGGTCGATGATGGAG
ATCAGCCTGGGGTGGCGGTGTCGGAAGCAGACGGCGGCGAGGGCCGGGAA
ATGGCTGGCGGTGCCGGGGACGGGATGGAAGTGGACGGCGATGATGTTGG
CGTCGCCACCGGCTTGGCCGAGGCTGACGATGAAGAGGGACAGGAGGGAG
CGGAGGCATCTCCGCCCAGGAGCCTCGCACCTGCCGCCGCAGTAGGTGGT
GATTTGTCCACCGGGGAGGAGGAGAATAACGGTGGTTGTAGAGGCGGCGG
CGACGTGGAGAAGGTGGGCGGCGGCGGCGGCGGTAGTGATGGCGGCGGCA
GGGTGGAAGGCAGGACAGAAGCCATTGTCGAGGCGATTGGCGAGGAGGAG
GTGTCCCGGGCGGGCGGGGGCGGGGATACCGATGCCGATGAAGAAACGAA
GAAGAAGAAAAAGAGCAGCAAGAAGGACAGGGGGAGCAGCAGCAGCGGGC
GCAAGAAGTCTTCTCGGCGCAGCAGCGACAAGTAGCCCTAAAAGAGCGGG
CGTGTTGCGGTCGTGGGCGAGCGCCGGACAGGATATCCTGGACAAGTCCA
TCCGGCTGAACTCTATGTTGTTTCGTGTGTTTGTGTATCGACCGTGCTTA
CCGGCGGACCAACTAAAAGACGTCGGTCTTTCAGGAGCGGGATGTGTGTG
TTTGCTTGGCGCGTGTTGATTAAGTTTTTTTTTTATGCGGAAGTGAGGGG
ACGGGAAGAACGGAGGCGGCGCGCGCGGTTCGTGCCCTGGAGAGATGGAG
GAACCGAGTACTGTGACAGGATTTGCAGCGGTACGTATCACGGGTAGATG
AGCGGGGGCGGACGAGGTCGCTGAGCAGCAGTCCGGGGAACGCAGCGATT
CCACCTTTCTCCACAACGGGTTGGGTTTTGCCGTCGTTTGTCGTTTTCGG
GGCACAACCACCTGGGAACAAGTATTTCAGTGCGCTCGACAGCTACCTCT
TTCCGTTTAGAAAGGATTCGCTGCCTGGTGTGCTGCATTCGCCGTTAGGT
ACGTCAGCCCCGTTACCCGTGTCCAATGAGTCCAGGACGGAAGCCTGAGG
AGTTGAAATCGGAAGATTGCGGTGCGGTGGCGTGTTTGTCGGGTGCCGAT
GTGCCTCGGTTCTTTGATCGTAACAGAGTTTTGATCTCCGGTTGTCTTGG
CCCCGATAAAGAAGCTCTCGTGAGTCGACAGGCTAAACCGCAGGGTTCCA
TGGAAACTACTGCGTGCGAGTGGGAGAATATGGCGAAATCACTTACCAGG
AGACGTACAAATGTGGGTAGAGGTGGAGCTAGCTATAATTGCCGTTTCGG
CTTCGTATGAGGTCTTCCGTGAATCCCAAGGCGTGCTTAGTCCGTAGTTT
CCGGGCCGAGCGACAAGGGCTCCTTTTGCCTTTTGGATTGCACAGCGTTG
GTTCCTGGGCGATACGCAACCCGTCGTGGGTGTTGTCATCGCGACCATGC
ATGTAGAGCTTGCGTCGCGGGTTGAAGGTTTGAAGTAGTCCTGCAGTTAC
GCGGGAAAGTGATGTTGAAACCGCTACCCCGCGAGTAAAGTGGCAGGTTG
GGTTTGAACTGAGAACTGATCCTGCAGTTGCGCGGGAAAGTCACCTGAAA
ACGATCCACCGCGAGTAGGGAAACCGCCTGGTTTTCCCTTCCCACACTGT
GATGACTTGAGTTTGCAAGGCAGGCGATGTTATCGGGCTTCCGTTTGGGT
AAGGGAAATGCAAAAAAATACATTACTAATCTATC
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig113:330..9364+

>mRNA_Ecto-sp13_S_contig113.1152.1 ID=mRNA_Ecto-sp13_S_contig113.1152.1|Name=mRNA_Ecto-sp13_S_contig113.1152.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=2517bp|location=Sequence derived from alignment at Ecto-sp13_S_contig113:330..9364+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGGATTTCGACCGGAATGCCATCGGATCTCTGGAGGACGTCCATCGGGA
ATGGGACAGCCTCGAGACCGAGCGTGCCAAGGTGGAAGACAAGCTGCGGA
GTTTCGACGCAGCGGCTGGGGGTAGGGGCGGTGGCTTTGGCGGGCGGGGG
GTAAGGGTTATTGGCGGCGGCAGGGGTAGGGACAGGAGTGGCGGGGGAGA
CTGGCGCAGGGATAGGCCCCGCGACACCAGCAATGGCGGTCGCGAGGAGG
CCCCGCACAGACCTTCCCAAGTATCGCGAGACAAGTACCGCGCGATGTCG
GGTACGTGGGAGCGAGGGCTAGACCCTGCAACGGCTGCCCTGGACGATGA
CGCCCCGCCGGAAGGCAGGGAGAGGGGCGGCGGCGGGACAGCGGGGCTTC
GGGGAGGCACCAAGGGTTCCATCGCGCACTACCAACGCCCCGCAGACAGC
CACGGTGACAGGAGGGGACGGGGTAGAGACCGCGGCGTTGCTCCAATGCA
TGGAGCTGGTGTGCGGGGAGACAGGGACGGTATGCGGGGCGATAGGACCG
GCGACAGGGACGTCTTCGCGCGGGGAGGACGAGGCGGAACGCGGGGTGAT
ACGGCGCGGGGAGACTTCGATCGGTTCAGGAACGGTGGCTCGGGGGGACG
AGGAGGAGGGTCGCGGGCACGCCGCGAGTCCAACGGCGTCGGGAGTGGCG
GCGGTGAGCCGGAAGCGAAGCGACCTCGGGTGACGAGCACGATCGTCGTC
GCGGGTGATGAGGACACCATCGGGGACGGGACGATGGAAAACGGCGAGCT
GGACCGTTGGAACACGGAGCTTCCCCCCGCATCGCCACCACCAGCGGCAG
AAGCTGCTGTCGCAACAGACATAGCAGCGACGACAACAACAACTCCAGAC
GACGACCTCGTGGACTACGAGCCGGAGGCAGAACCGGAGGAGGACACCGC
TAGCGGGCCTCCACCCGCCGCTGCCGCCGCCGCCCCCCCCGGCGAGGTAA
CGGGCCAGGAAGGCGATCAGCAGCCCATGGAAGAGGACGACGGCTGCGGG
CGTAGGCCCGTCGGCAAGATCGTGGTCGTTTCGGGCGGCGGCCCTAGAGG
GCGCGCTGCCAACATCGGCGGCGTCGCCGGCAGCGGCGGCGGGCGCGGGA
GCCTGGCTAGCAGGTTGGGCCCTCCCATGGGGAGGCCGGTGATGGTGAAA
GGGGGTTCGGGGGACAACGGCGGGGAAGGGGGCTTTGTGCCTCTGTCGGA
GAGACCCATGCCGGAGCCCTCGAAGCCAGAGCTTCACGTGGCGTACAAGG
ACGGGGACACCAAGAAGCGAAACAGGAGGATGTTCGGTGGCCTCATGGCC
CACCTCGGCCGAGCACGACAGCAGCTAGACCATGACAAGTCCATTATACA
GCGGCAGAAGAGCGTTGTCGAGATGGCGGCGATCAGGCAGGCGCAAGAGA
AGAGGCAACGCCGACAGACGGCTGAAAATGCGGCTCGTGAGGAGCGCGAT
GAGGAGCTGCACAAGCGCGACTACATCCGCGCGAGGCAGCAGAAGACCTT
GGTTGCTCTTCACGGAAACGGTTTCATCGCCAACCAGGAGCAGCTCAAGG
GGTTCATCTTCACAAAGACCCTGCCCAAGCTAGCTTGGTCACCCAACGAG
CATACCGACGTTACCCAGGCTCTGCTGGCAGACTCTAGCAAGGAGATTGA
CGGTAACATCGAACGGAGGAAAGAGGCCGACGACAAGGAGTTCAAGGAGA
TCGACGATTCGGTTGCAGAACGAGCCGCGACGCGGGCCAGCCGGAGGATG
GCACACGCTCCGGGGGTGCGGCAGATGGGGGTGCGGCAAGACATGGACGC
TAACCCTCACGGAGAAGGCAACGGGCATCGCTGGCAGATGGCGGCGGGAG
AGAGTGACACAAATGGGGAGGGTCCCGGCCAAGCCCACACGGTCGCTCAG
GATGATGACGACGGCAATGCTGGTGCGACAGAGGACATTGGCGGCGCCCC
GTCCATGAGCCAAGACGTGGTCGATGATGGAGATCAGCCTGGGGTGGCGG
TGTCGGAAGCAGACGGCGGCGAGGGCCGGGAAATGGCTGGCGGTGCCGGG
GACGGGATGGAAGTGGACGGCGATGATGTTGGCGTCGCCACCGGCTTGGC
CGAGGCTGACGATGAAGAGGGACAGGAGGGAGCGGAGGCATCTCCGCCCA
GGAGCCTCGCACCTGCCGCCGCAGTAGGTGGTGATTTGTCCACCGGGGAG
GAGGAGAATAACGGTGGTTGTAGAGGCGGCGGCGACGTGGAGAAGGTGGG
CGGCGGCGGCGGCGGTAGTGATGGCGGCGGCAGGGTGGAAGGCAGGACAG
AAGCCATTGTCGAGGCGATTGGCGAGGAGGAGGTGTCCCGGGCGGGCGGG
GGCGGGGATACCGATGCCGATGAAGAAACGAAGAAGAAGAAAAAGAGCAG
CAAGAAGGACAGGGGGAGCAGCAGCAGCGGGCGCAAGAAGTCTTCTCGGC
GCAGCAGCGACAAGTAG
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