mRNA_E-siliculosus-1a_M_contig94.17447.1 (mRNA) Ectocarpus siliculosus Ec864m_EcPH12_78m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_M_contig94.17447.1
Unique NamemRNA_E-siliculosus-1a_M_contig94.17447.1
TypemRNA
OrganismEctocarpus siliculosus Ec864m_EcPH12_78m male (Ectocarpus siliculosus Ec864m_EcPH12_78m male)
Homology
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BLAST of mRNA_E-siliculosus-1a_M_contig94.17447.1 vs. uniprot
Match: A0A6H5KPM9_9PHAE (Thioredoxin domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KPM9_9PHAE)

HSP 1 Score: 340 bits (871), Expect = 2.960e-111
Identity = 174/181 (96.13%), Postives = 178/181 (98.34%), Query Frame = 3 
Query:   93 MVLLRNVAVLVVLSLSAAVVSAFVPGGNPGGMAKAAAVKLEGGGTKTPIRAITHPRTNVKEIASVSNTEEFESLLGLARPKQVVAIWFHASWCRKCKYIGTRLKRLDEHLPAYLTNNLVVVSVDVNQVAQVPQRQKIKDLPTIQFFVDGGVVGSYVANDRGEAFYANMEKHLRAALGEDVE 635
            MVL RNVAVLVVLSLSAAV SAFVPGGNPGGMAKAAAV+LEGGGTKTPIRAITHPRTNVKEIASVSNTEEFESLL LARPKQVVAIWFHASWCRKCKYIGTRLKRLDEHLPAYL+NNLVVVS+DVNQVAQVPQRQKIKDLPTIQFFVDGG+VGSYVANDRGEAFYANMEKHLRAALGEDVE
Sbjct:    1 MVLSRNVAVLVVLSLSAAVASAFVPGGNPGGMAKAAAVQLEGGGTKTPIRAITHPRTNVKEIASVSNTEEFESLLRLARPKQVVAIWFHASWCRKCKYIGTRLKRLDEHLPAYLSNNLVVVSIDVNQVAQVPQRQKIKDLPTIQFFVDGGMVGSYVANDRGEAFYANMEKHLRAALGEDVE 181
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BLAST of mRNA_E-siliculosus-1a_M_contig94.17447.1 vs. uniprot
Match: B7ZGM8_9PHAE (Thioredoxin-like 5 n=1 Tax=Laminaria digitata TaxID=80365 RepID=B7ZGM8_9PHAE)

HSP 1 Score: 208 bits (530), Expect = 2.340e-60
Identity = 104/149 (69.80%), Postives = 123/149 (82.55%), Query Frame = 3 
Query:  189 AKAAAVKLEGGGTKTPIRAITHPRTNVKEIASVSNTEEFESLLGLARPKQVVAIWFHASWCRKCKYIGTRLKRLDEHLPAYLTNNLVVVSVDVNQVAQVPQRQKIKDLPTIQFFVDGGVVGSYVANDRGEAFYANMEKHLRAALGEDVE 635
            A A   + + GGT+TPI AI  P T  KEI +V N EEF+ ++  ARPKQVVA+WF ASWCRKCKYIG+RL+RLDEHLPAYLTNNLV+VS+DVN+VA+VPQRQKIKDLPTIQFFV+G  VGSYVA D+GEAFY+NME+HLRAA  +  E
Sbjct:   45 AVATGARRDVGGTRTPISAIASPVTT-KEIQTVGNVEEFDRIV--ARPKQVVAVWFQASWCRKCKYIGSRLRRLDEHLPAYLTNNLVLVSIDVNKVAEVPQRQKIKDLPTIQFFVEGKEVGSYVATDKGEAFYSNMERHLRAAQEDTTE 190
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BLAST of mRNA_E-siliculosus-1a_M_contig94.17447.1 vs. uniprot
Match: A0A6T6LFR0_9RHOD (Hypothetical protein n=1 Tax=Timspurckia oligopyrenoides TaxID=708627 RepID=A0A6T6LFR0_9RHOD)

HSP 1 Score: 70.1 bits (170), Expect = 2.220e-10
Identity = 31/69 (44.93%), Postives = 48/69 (69.57%), Query Frame = 3 
Query:  333 KQVVAIWFHASWCRKCKYIGTRLKRLDEHLPAYLTNNLVVVSVDVNQVAQVPQRQKIKDLPTIQFFVDG 539
            K +VAI FHA+WCRKC ++  ++++L    P     N++   VD+N VA++P+RQ+I ++PTIQ F DG
Sbjct:   79 KSIVAIAFHAAWCRKCIWLIPKIEKLAVEYP-----NILFAKVDINTVAEIPRRQEIHNIPTIQIFQDG 142
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BLAST of mRNA_E-siliculosus-1a_M_contig94.17447.1 vs. uniprot
Match: A0A5D3DCH1_CUCME (Thioredoxin H1-like n=3 Tax=Benincaseae TaxID=1003877 RepID=A0A5D3DCH1_CUCME)

HSP 1 Score: 53.1 bits (126), Expect = 5.160e-5
Identity = 29/101 (28.71%), Postives = 54/101 (53.47%), Query Frame = 3 
Query:  306 ESLLGLARPKQVVAIWFHASWCRKCKYIGTRLKRLDEHLPAYLTNNLVVVSVDVNQVAQVPQRQKIKDLPTIQFFVDGGVVGSYVANDRGEAFYANMEKHL 608
            + LL   +  ++V + F A+WC  C+ +   LK L   +    +NN++V+ VDV+++  V     +  LP+ QFF +G +V  +V   R +  +  + KH+
Sbjct:   18 QQLLKAKQYNKLVVVNFTATWCAPCRGMAPVLKDLANKM----SNNVIVLKVDVDELMSVANEYGVGALPSFQFFKNGKLVDKFVGA-RKDVLHKTVSKHV 113
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_M_contig94.17447.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a male vs UniRef90)
Total hits: 4
Match NameE-valueIdentityDescription
A0A6H5KPM9_9PHAE2.960e-11196.13Thioredoxin domain-containing protein n=2 Tax=Ecto... [more]
B7ZGM8_9PHAE2.340e-6069.80Thioredoxin-like 5 n=1 Tax=Laminaria digitata TaxI... [more]
A0A6T6LFR0_9RHOD2.220e-1044.93Hypothetical protein n=1 Tax=Timspurckia oligopyre... [more]
A0A5D3DCH1_CUCME5.160e-528.71Thioredoxin H1-like n=3 Tax=Benincaseae TaxID=1003... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_M_contig94contigE-siliculosus-1a_M_contig94:521063..525203 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a male vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec864m_EcPH12_78m male2021-02-24
Properties
Property NameValue
Start1
Stop1
Cds size546
Model size1843
Exons3
Ec32 orthologEc-27_001010.1
Ec32 ortholog descriptionThioredoxin-like fold
Hectar predicted targeting categorychloroplast
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_M_contig94.17447.1prot_E-siliculosus-1a_M_contig94.17447.1Ectocarpus siliculosus Ec864m_EcPH12_78m malepolypeptideE-siliculosus-1a_M_contig94 521155..523998 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622936504.503062-UTR-E-siliculosus-1a_M_contig94:521062..5211541622936504.503062-UTR-E-siliculosus-1a_M_contig94:521062..521154Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig94 521063..521154 +
1696251108.2138078-UTR-E-siliculosus-1a_M_contig94:521062..5211541696251108.2138078-UTR-E-siliculosus-1a_M_contig94:521062..521154Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig94 521063..521154 +
1622936504.5800557-UTR-E-siliculosus-1a_M_contig94:523998..5252031622936504.5800557-UTR-E-siliculosus-1a_M_contig94:523998..525203Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig94 523999..525203 +
1696251108.271208-UTR-E-siliculosus-1a_M_contig94:523998..5252031696251108.271208-UTR-E-siliculosus-1a_M_contig94:523998..525203Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig94 523999..525203 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622936504.5258014-CDS-E-siliculosus-1a_M_contig94:521154..5214001622936504.5258014-CDS-E-siliculosus-1a_M_contig94:521154..521400Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig94 521155..521400 +
1696251108.2329593-CDS-E-siliculosus-1a_M_contig94:521154..5214001696251108.2329593-CDS-E-siliculosus-1a_M_contig94:521154..521400Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig94 521155..521400 +
1622936504.544071-CDS-E-siliculosus-1a_M_contig94:523325..5234361622936504.544071-CDS-E-siliculosus-1a_M_contig94:523325..523436Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig94 523326..523436 +
1696251108.248433-CDS-E-siliculosus-1a_M_contig94:523325..5234361696251108.248433-CDS-E-siliculosus-1a_M_contig94:523325..523436Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig94 523326..523436 +
1622936504.563038-CDS-E-siliculosus-1a_M_contig94:523809..5239981622936504.563038-CDS-E-siliculosus-1a_M_contig94:523809..523998Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig94 523810..523998 +
1696251108.2598214-CDS-E-siliculosus-1a_M_contig94:523809..5239981696251108.2598214-CDS-E-siliculosus-1a_M_contig94:523809..523998Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig94 523810..523998 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_M_contig94.17447.1

>prot_E-siliculosus-1a_M_contig94.17447.1 ID=prot_E-siliculosus-1a_M_contig94.17447.1|Name=mRNA_E-siliculosus-1a_M_contig94.17447.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=polypeptide|length=182bp
MVLLRNVAVLVVLSLSAAVVSAFVPGGNPGGMAKAAAVKLEGGGTKTPIR
AITHPRTNVKEIASVSNTEEFESLLGLARPKQVVAIWFHASWCRKCKYIG
TRLKRLDEHLPAYLTNNLVVVSVDVNQVAQVPQRQKIKDLPTIQFFVDGG
VVGSYVANDRGEAFYANMEKHLRAALGEDVE*
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mRNA from alignment at E-siliculosus-1a_M_contig94:521063..525203+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_M_contig94.17447.1 ID=mRNA_E-siliculosus-1a_M_contig94.17447.1|Name=mRNA_E-siliculosus-1a_M_contig94.17447.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=mRNA|length=4141bp|location=Sequence derived from alignment at E-siliculosus-1a_M_contig94:521063..525203+ (Ectocarpus siliculosus Ec864m_EcPH12_78m male)
GTAGCCCACCAACTGCAAGCCGACCACGTTGCGCTCTCGCTTCTGAGAGA
GGGGCGACAACACACGCCACACACACCACACATCGCGGCATCATGGTGTT
GTTGAGGAATGTGGCTGTGCTCGTCGTGCTGTCGTTGTCAGCAGCGGTAG
TAAGTGCCTTTGTCCCGGGGGGAAACCCGGGCGGCATGGCAAAAGCCGCT
GCCGTTAAATTGGAGGGCGGCGGCACCAAGACGCCCATCAGAGCGATAAC
CCACCCCCGGACGAACGTGAAGGAGATTGCCAGCGTGAGCAATACGGAGG
AGTTCGAGAGCTTGCTGGGGTTGGCGAGACCGAAACAGGTGGGCTTGAAA
GCAAGAAGGGGGGTAAGAGCCCGAATTTAGACTTGAGACTTGAAACCAAC
CAAGAACGTACCTTGCGGAGAACAGAGGAAGCACTTTTCTCGTAATTTGT
CCATCGGCGCGCACATTCTCAAGAGGTTGGTTGTTGCTGAAGAGACTCAA
TGCTCTTGTCCCTGCTGCTCCTCATTCTCACCCGGCTCACGGTAGCCCTT
CCGAACTAGTACCACGTGGCACGGTCTTTCGAAGATTGGGTGGCACAGGC
TGCGCCCAATAACCACAAGGACCAGTGGACCAGTCGCTGTGCTGCTTCAA
ATGCGACGTATGTGCTTCAATTTGACAAACGTACTTTTGGTGCCAGTTGA
TAAACTGATTGGCCGATCGAGAGCGCCTCAAGGGCAAGTTCCCCCCCCCC
CCCGCCCCCGGTTGCTTAAGCGGTTGGTTTGCGATTGGCGTAGTGGCCTC
CGCGATGGTATGCACCCGCTCTTTTGGTCGCGCACGCATGCTAGACCACG
CCCGCCGCTGCCCTCTTGTCACTCCGATTGTTGCAATTCCGAGTGTCCTA
GTCCTCTTCGTTTGGTACGAACGTAAGTTGCTCTTCGGTACCCTTTCATG
GAAATAGCATTGTAGAACCCACGAGGGGGGGAACAAGCCCCGATGCTGGA
CTCAAGTGGGATGGATTGAAGCCCTTACAAATGCAGTTTAGGCTCAGTGC
CCGCCCGTGTGAGACAACCAACGCCTGGTCCACGTTTTGCAACGCCGATG
CAGGAGCAGCCACAACGGAAAAGTTTGGGATTTTTTTTTTTGGAAGCTCC
ACATCAACATCAACCCGTCCCCTACCCCCCGGGCCGCTCCCCCCCTAGAT
CCGCCTCCCATCCCCTCCCTCGGTGTATCGTTCAGTCTTTTAGTGTGTGT
ATACATGGACTCTCGTATGACCGCAGGTAACGCATTGGCGGCACACGCGA
GGCGTACGTGCCCCCCAGGTTGTTCCAGTTACTACTTCGTAGAGATAATA
TCTAGAACCTTTGTTTTGGACTCCGGTCTTTTCATCCTTGGGGTTCAACC
CGCCCGGAAATGCGAGGCATTGAAGTTTACTATAGGTCCTCTTGTGGTTA
CCGTACTCAACGAAGACGCGAGCAACACATCTCTACAATTCTAGCACGCG
TCCGTATCTACACTTGCACGAGTGATGGCAGGTGTGCTCGGGTCATTGAC
CGCAAATGGGCTAGCATGCTTGTACCATACACCAGCAGGCAGCAAGCAGC
ATTCGTCACTAAAGAAATCTCGAATCTGACATAGCTTTTATGCTGGAAAA
TTCTCAACAGTATGATACCACCACCCCGAGACATCGCTAAATAGAGGATT
TTGTCATCTGTTGAGGTTCCTGCGACCAGACGCATCGAGTCGCCTCGCGT
AGTCGTGCGTGGTACATGCTGCTGTAACCGCACTGCGTGGATTGAACGGG
TGCGCGGCAATTGATGAGAACGGCATGTACCATCGACGAGAGAACTCTCG
CGTTCCCACAAGTTCCTGCCGTTTCGTGACGTTTTTAAAAATGGGAAATA
ACAGCATGGTGTCCGTTGTTTGGATTTCGAGGAGGTCGATAAGGGTCGTG
GTGGTGGTGGTGAACGGTATTGCACGCGCGAAAGCGGTTCGTTTTTGAAA
CAGCATCTTGGCAGTACGACTGCTTGATTGCAGCAAGCACGCCTTTGGAT
ATACCGTTGCCGCTTGGCCTTTTGCCACACTTCTCTTTCACAGAAAAATA
AATTCGAAGGGAGACGCGTCCAGGAAAACATGCCTTCAAGTACCAATTCT
GACGTCCTTAAGAAATGCCACAGGCACACACACTTGTGTGACGAAAACGT
ATTCCTATTCCTTGTAACTTGAACGCTTCTCTCTGCTCGCTGTCTGTTTT
TCTGATTTTTTAGGTTGTGGCGATCTGGTTCCACGCAAGCTGGTGTCGGA
AATGCAAGTACATCGGTACGAGATTGAAACGCCTGGACGAGCACCTCCCG
GCGTACTTGACGAACAATCTTGTGGTGAGCGAGAGATAGAGAGAGTGGGG
AGGGGGGGGATCAGAGGGAGGGAACCAAGACGGGGAGGGAGAACGACAGA
TGTGATGTAGGCAGGCGTCGGAGCACACGCAAGATCATACTGAAATACTG
CTGTAGTGGTTGATTTTTGCTGTCGGGCACCACAGCCATCTCTTGTCTCT
TCCTTCCGGTGTGCTCAACTCTTTTTCGTTCCTGGCGACTCCCCAAGCTC
CTGGTGTATTGACGTTTGCTGCAAGTAGGTATCCTAATTTGATTTGGTGA
AATCACTTTCGCCTCCACTAGTCTCTGTCTCTCTCCAGCACTAATCTCCT
TTCGTCGTCTGCACTCTCCCGCTCTCCTTTGTACCTGCCTTTTCTAGGTG
GTGAGCGTCGATGTCAACCAAGTGGCACAGGTCCCGCAGCGACAAAAGAT
CAAGGACCTCCCGACGATCCAGTTCTTCGTGGACGGAGGCGTGGTCGGTT
CGTACGTAGCCAACGACAGGGGGGAGGCGTTCTACGCGAACATGGAGAAG
CACTTGCGTGCGGCTCTCGGCGAAGACGTGGAGTAGTGTGCAGCGCAGAG
CGTACTCGTTTTGGCCCCCTTCCTCCTTGGGACCACACCTTCTTGAAACC
TCCTTCCTTTTGGTTGCTCAGAGAGGCAGACGAAGCAGGCGTTTTGTTCG
TCACGGGGAAGGAGAGGGGGAGCGTTATTTGATTTGATAGGGTTTTGTAG
GATGTCCGCGACTCTAAAAATTGTTGACGTCGGAAGGCTAGCCGGCTCCT
TCGCCATTGTTCCAGCGCAAGAGAGGACGGGGGGGGATTTTCCGGCCAAC
AATCGTCTGTAAGCATACTTGCACGGCAGTTTGCGTTGGTGATCGATAGA
GGACGCAACACGAATGTCGTAAGCTGTGTTTGTAAAGTTTTCGTTTTTAT
TTGAGAAGGGAGGAAGGGAAGTGTGTAGTTGAGTTTCGTATTCATTTCGT
CTAGGTTTCAAAAATAGTTTGATATTTGTTGTTGGTTTATTTCGACCCTA
GGAAATACGCGGAATCTCAAGAGTGCGTCGCGCCCCAAGTATGTTGTCGT
ACGCCATGGAAGTGTTGTTAGAAGCGTAATAACTGGGACGACTTGTGTTC
CCAAGCACGTGTTGTAGCACTCAAGAGCAGCAAAACCTGCAAGTGATTTC
GCCCCGGACTTTCGATATTCTCGCATGCATATGGTGCTGTGCTTGCCACC
GCCCTTTGTGGTTGCTGAACAGCACGTATGAGCTGGCGATGCCCGTGTAA
TGCATCGCTGTTGCGCCTTGCTTTTTTTGCTCAGGAGGGCGTGGCATTCC
GAGATTTCGAAACAGGTTTTTTGGGAATTGTGTCTTGTTTTTGTCGTTTC
CCACCCTGCCGGGACGATTGTGTTGTGCCCAAGACATGACGTGGCCTTGG
CGTATCTCGTTTTGCAAGTTGCCTCTCGGCGGCTAGAATTTGGCGTGTGG
CTCTGTTGGGGACTCAGGAGTTTGGAGAGTGTCGTGGAAACACATGAGAA
ACTAGAAAGTTATGCGGAGAGACCAACGTCCAGTTCGTTCTTGTTTTTTT
AGCACTTTCGGCGGGCGGGAGTTGGGCGTTGGACGGAGCCTCCCTCTTGT
TGCTAGTATCGTGAGTCGATGTTGCCATTTTCTTGTCAACATACTCGAGA
ATTTTTTTTTCGGAGAAAAATGGTTTGGTGTTATTGTACAGACGCCAATA
GCTATGCACAAGGAAGTATTCCACTGCATGAACGAATCAGA
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Coding sequence (CDS) from alignment at E-siliculosus-1a_M_contig94:521063..525203+

>mRNA_E-siliculosus-1a_M_contig94.17447.1 ID=mRNA_E-siliculosus-1a_M_contig94.17447.1|Name=mRNA_E-siliculosus-1a_M_contig94.17447.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=CDS|length=1092bp|location=Sequence derived from alignment at E-siliculosus-1a_M_contig94:521063..525203+ (Ectocarpus siliculosus Ec864m_EcPH12_78m male)
ATGGTGTTGTTGAGGAATGTGGCTGTGCTCGTCGTGCTGTCGTTGTCAGC
AGCGGTAGTAAGTGCCTTTGTCCCGGGGGGAAACCCGGGCGGCATGGCAA
AAGCCGCTGCCGTTAAATTGGAGGGCGGCGGCACCAAGACGCCCATCAGA
GCGATAACCCACCCCCGGACGAACGTGAAGGAGATTGCCAGCGTGAGCAA
TACGGAGGAGTTCGAGAGCTTGCTGGGGTTGGCGAGACCGAAACAGATGG
TGTTGTTGAGGAATGTGGCTGTGCTCGTCGTGCTGTCGTTGTCAGCAGCG
GTAGTAAGTGCCTTTGTCCCGGGGGGAAACCCGGGCGGCATGGCAAAAGC
CGCTGCCGTTAAATTGGAGGGCGGCGGCACCAAGACGCCCATCAGAGCGA
TAACCCACCCCCGGACGAACGTGAAGGAGATTGCCAGCGTGAGCAATACG
GAGGAGTTCGAGAGCTTGCTGGGGTTGGCGAGACCGAAACAGGTTGTGGC
GATCTGGTTCCACGCAAGCTGGTGTCGGAAATGCAAGTACATCGGTACGA
GATTGAAACGCCTGGACGAGCACCTCCCGGCGTACTTGACGAACAATCTT
GTGGTTGTGGCGATCTGGTTCCACGCAAGCTGGTGTCGGAAATGCAAGTA
CATCGGTACGAGATTGAAACGCCTGGACGAGCACCTCCCGGCGTACTTGA
CGAACAATCTTGTGGTGGTGAGCGTCGATGTCAACCAAGTGGCACAGGTC
CCGCAGCGACAAAAGATCAAGGACCTCCCGACGATCCAGTTCTTCGTGGA
CGGAGGCGTGGTCGGTTCGTACGTAGCCAACGACAGGGGGGAGGCGTTCT
ACGCGAACATGGAGAAGCACTTGCGTGCGGCTCTCGGCGAAGACGTGGAG
TAGGTGGTGAGCGTCGATGTCAACCAAGTGGCACAGGTCCCGCAGCGACA
AAAGATCAAGGACCTCCCGACGATCCAGTTCTTCGTGGACGGAGGCGTGG
TCGGTTCGTACGTAGCCAACGACAGGGGGGAGGCGTTCTACGCGAACATG
GAGAAGCACTTGCGTGCGGCTCTCGGCGAAGACGTGGAGTAG
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