mRNA_E-siliculosus-1a_M_contig93.17367.1 (mRNA) Ectocarpus siliculosus Ec864m_EcPH12_78m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_M_contig93.17367.1
Unique NamemRNA_E-siliculosus-1a_M_contig93.17367.1
TypemRNA
OrganismEctocarpus siliculosus Ec864m_EcPH12_78m male (Ectocarpus siliculosus Ec864m_EcPH12_78m male)
Homology
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17367.1 vs. uniprot
Match: A0A6H5L128_9PHAE (COX assembly mitochondrial protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L128_9PHAE)

HSP 1 Score: 174 bits (442), Expect = 1.840e-54
Identity = 83/88 (94.32%), Postives = 87/88 (98.86%), Query Frame = 2 
Query:  137 MHPPLLRPHPSCHEEVKVLMACHEENPYGKFFGACNDLKIALDSCFVHEKEEKRRKNMAKARRFDAGFQKELELRRKELDQEQQQAGR 400
            MHPPLLRPHPSCHEEVK+LMACHEENPYGKFFGACNDLK+ALDSCFV EKEEKRRKN+AKARRFDAGFQKELELRRKEL+QEQQQAGR
Sbjct:    1 MHPPLLRPHPSCHEEVKMLMACHEENPYGKFFGACNDLKLALDSCFVLEKEEKRRKNLAKARRFDAGFQKELELRRKELEQEQQQAGR 88
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17367.1 vs. uniprot
Match: F0Y773_AURAN (COX assembly mitochondrial protein n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y773_AURAN)

HSP 1 Score: 97.8 bits (242), Expect = 3.740e-24
Identity = 44/75 (58.67%), Postives = 54/75 (72.00%), Query Frame = 2 
Query:  137 MHPPLLRPHPSCHEEVKVLMACHEENPYGKFFGACNDLKIALDSCFVHEKEEKRRKNMAKARRFDAGFQKELELR 361
            MHPPL RPHP C   V +L+ CH+ENPYGKF+GACND K A+D CF  EKEEKRR N  K R F+  ++K+ + R
Sbjct:    1 MHPPLYRPHPKCAALVDLLVKCHDENPYGKFWGACNDEKAAMDWCFKEEKEEKRRANFEKTRSFNEEWRKKQDAR 75
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17367.1 vs. uniprot
Match: A0A8J2WQW2_9STRA (COX assembly mitochondrial protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2WQW2_9STRA)

HSP 1 Score: 95.5 bits (236), Expect = 3.290e-23
Identity = 42/66 (63.64%), Postives = 50/66 (75.76%), Query Frame = 2 
Query:  137 MHPPLLRPHPSCHEEVKVLMACHEENPYGKFFGACNDLKIALDSCFVHEKEEKRRKNMAKARRFDA 334
            MHPPL RPHP C   V  L+ CH+ N YGKF+GACND K A+D+CF  EK+EKRR+N+ KAR FDA
Sbjct:    1 MHPPLYRPHPKCAALVDALVECHDANVYGKFWGACNDQKAAMDACFKAEKDEKRRENLRKARAFDA 66
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17367.1 vs. uniprot
Match: A0A3M6VJT8_9STRA (COX assembly mitochondrial protein n=1 Tax=Peronospora effusa TaxID=542832 RepID=A0A3M6VJT8_9STRA)

HSP 1 Score: 94.7 bits (234), Expect = 7.190e-23
Identity = 41/76 (53.95%), Postives = 55/76 (72.37%), Query Frame = 2 
Query:  137 MHPPLLRPHPSCHEEVKVLMACHEENPYGKFFGACNDLKIALDSCFVHEKEEKRRKNMAKARRFDAGFQKELELRR 364
            MH PL RPHP C  E+K L+ CH+ NPY KFFGAC+D+K ALD CF HEKE  R +N+ +A+  DA  +++++ RR
Sbjct:    1 MHTPLDRPHPDCQSEIKALLQCHDNNPYAKFFGACSDVKTALDWCFKHEKERIRAENLKRAKASDAFVKQKMQERR 76
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17367.1 vs. uniprot
Match: K3WST6_GLOUD (COX assembly mitochondrial protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WST6_GLOUD)

HSP 1 Score: 92.0 bits (227), Expect = 8.730e-22
Identity = 45/86 (52.33%), Postives = 57/86 (66.28%), Query Frame = 2 
Query:  137 MHPPLLRPHPSCHEEVKVLMACHEENPYGKFFGACNDLKIALDSCFVHEKEEKRRKNMAKARRFDAGFQKELELRRKELDQEQQQA 394
            MH PL RPHP C E +  L  CHEENP  KFFGACN+ K+ALD CF  EKE KR +N+ +AR  DA  +++++ RR+   Q  Q A
Sbjct:    1 MHAPLDRPHPDCQEVIMALNLCHEENPRMKFFGACNEAKVALDKCFKKEKERKRDENLRRARASDAYVRQKMKERREREAQAAQDA 86
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17367.1 vs. uniprot
Match: G4ZUD5_PHYSP (COX assembly mitochondrial protein n=1 Tax=Phytophthora sojae (strain P6497) TaxID=1094619 RepID=G4ZUD5_PHYSP)

HSP 1 Score: 91.3 bits (225), Expect = 2.380e-21
Identity = 43/83 (51.81%), Postives = 55/83 (66.27%), Query Frame = 2 
Query:  137 MHPPLLRPHPSCHEEVKVLMACHEENPYGKFFGACNDLKIALDSCFVHEKEEKRRKNMAKARRFDAGFQKELELRRKELDQEQ 385
            MH PL RPHP C  E+K L+ CHEENPY KFFGAC D+K ALD CF  EK   R +N  +A+  DA  +++++ RR  +  EQ
Sbjct:    1 MHTPLDRPHPDCQAEIKALLECHEENPYAKFFGACGDVKTALDWCFREEKVRIRSENFQRAKASDAYVRQKMQERRDRVAAEQ 83
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17367.1 vs. uniprot
Match: A0A6A3SY18_9STRA (COX assembly mitochondrial protein n=2 Tax=Phytophthora TaxID=4783 RepID=A0A6A3SY18_9STRA)

HSP 1 Score: 89.4 bits (220), Expect = 1.250e-20
Identity = 41/83 (49.40%), Postives = 55/83 (66.27%), Query Frame = 2 
Query:  137 MHPPLLRPHPSCHEEVKVLMACHEENPYGKFFGACNDLKIALDSCFVHEKEEKRRKNMAKARRFDAGFQKELELRRKELDQEQ 385
            MH PL RPHP C  E+K L+ CH+ENPY KFFGAC ++K ALD CF  EK   R +N   A+  DA  +++++ RR  + +EQ
Sbjct:    1 MHTPLDRPHPDCQAEIKALLECHDENPYAKFFGACGEVKTALDWCFRQEKTRIRSENFKHAKASDAFVRQKMQERRDRMAEEQ 83
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17367.1 vs. uniprot
Match: A0A225VCF9_9STRA (COX assembly mitochondrial protein n=1 Tax=Phytophthora megakarya TaxID=4795 RepID=A0A225VCF9_9STRA)

HSP 1 Score: 89.0 bits (219), Expect = 1.460e-20
Identity = 41/86 (47.67%), Postives = 56/86 (65.12%), Query Frame = 2 
Query:  137 MHPPLLRPHPSCHEEVKVLMACHEENPYGKFFGACNDLKIALDSCFVHEKEEKRRKNMAKARRFDAGFQKELELRRKELDQEQQQA 394
            MH PL RPHP C  E+K L+ CH+ENPY KFFGAC ++K ALD CF  EK   R +N   A+  DA  +++++ RR  +  E+ +A
Sbjct:    1 MHTPLDRPHPDCQTEIKALLECHDENPYAKFFGACGEIKTALDICFREEKNRIRSENFKHAKASDAYVKQKMQERRDRVANEKAKA 86
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17367.1 vs. uniprot
Match: A0A0P1AB85_PLAHL (COX assembly mitochondrial protein n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1AB85_PLAHL)

HSP 1 Score: 88.6 bits (218), Expect = 1.950e-20
Identity = 40/76 (52.63%), Postives = 52/76 (68.42%), Query Frame = 2 
Query:  137 MHPPLLRPHPSCHEEVKVLMACHEENPYGKFFGACNDLKIALDSCFVHEKEEKRRKNMAKARRFDAGFQKELELRR 364
            MH PL RPHP C  E+K L+ CH +NPY KFFGACND+K ALD CF  EK   R +N+  A+  DA  +++++ RR
Sbjct:    1 MHAPLDRPHPDCQIEIKALLDCHNDNPYAKFFGACNDVKSALDQCFKKEKIRIRSENLRHAKASDAYVRRKMQERR 76
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17367.1 vs. uniprot
Match: A0A2P4XXV8_9STRA (COX assembly mitochondrial protein n=1 Tax=Phytophthora palmivora var. palmivora TaxID=611791 RepID=A0A2P4XXV8_9STRA)

HSP 1 Score: 88.2 bits (217), Expect = 3.450e-20
Identity = 40/76 (52.63%), Postives = 50/76 (65.79%), Query Frame = 2 
Query:  137 MHPPLLRPHPSCHEEVKVLMACHEENPYGKFFGACNDLKIALDSCFVHEKEEKRRKNMAKARRFDAGFQKELELRR 364
            MH PL RPHP C  E+K L+ CHE NPY KFFGAC D+K ALD CF  EK   R +N   A+  DA  +++++ RR
Sbjct:    1 MHTPLDRPHPDCQTEIKALLECHESNPYAKFFGACGDVKTALDICFREEKNRIRSENFKHAKASDAYVKQKMQERR 76
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_M_contig93.17367.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5L128_9PHAE1.840e-5494.32COX assembly mitochondrial protein n=1 Tax=Ectocar... [more]
F0Y773_AURAN3.740e-2458.67COX assembly mitochondrial protein n=1 Tax=Aureoco... [more]
A0A8J2WQW2_9STRA3.290e-2363.64COX assembly mitochondrial protein n=1 Tax=Pelagom... [more]
A0A3M6VJT8_9STRA7.190e-2353.95COX assembly mitochondrial protein n=1 Tax=Peronos... [more]
K3WST6_GLOUD8.730e-2252.33COX assembly mitochondrial protein n=1 Tax=Globisp... [more]
G4ZUD5_PHYSP2.380e-2151.81COX assembly mitochondrial protein n=1 Tax=Phytoph... [more]
A0A6A3SY18_9STRA1.250e-2049.40COX assembly mitochondrial protein n=2 Tax=Phytoph... [more]
A0A225VCF9_9STRA1.460e-2047.67COX assembly mitochondrial protein n=1 Tax=Phytoph... [more]
A0A0P1AB85_PLAHL1.950e-2052.63COX assembly mitochondrial protein n=1 Tax=Plasmop... [more]
A0A2P4XXV8_9STRA3.450e-2052.63COX assembly mitochondrial protein n=1 Tax=Phytoph... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_M_contig93contigE-siliculosus-1a_M_contig93:461611..463847 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a male vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec864m_EcPH12_78m male2021-02-24
Properties
Property NameValue
Start1
Stop1
Cds size267
Model size420
Exons3
Ec32 orthologEc-15_002730.1
Ec32 ortholog descriptionCytochrome c oxidase biogenesis protein Cmc1-like
Hectar predicted targeting categoryother localisation
BRITEko00000,ko03029
Best eggNOG OGNA|NA|NA
Best tax levelPythiales
COG Functional cat.O
EggNOG OGs1MI5Q@121069,2CZWX@1,2SBZE@2759
EggNOG free text desc.CNPV141 HAL3-like domain protein (ISS). Source PGD
KEGG koko:K18172
Seed eggNOG ortholog65071.PYU1_T008030
Seed ortholog evalue9.5e-16
Seed ortholog score89.4
Taxonomic scopeEukaryota
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_M_contig93.17367.1prot_E-siliculosus-1a_M_contig93.17367.1Ectocarpus siliculosus Ec864m_EcPH12_78m malepolypeptideE-siliculosus-1a_M_contig93 461628..463711 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622936483.9919553-UTR-E-siliculosus-1a_M_contig93:461610..4616271622936483.9919553-UTR-E-siliculosus-1a_M_contig93:461610..461627Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig93 461611..461627 -
1696251098.9973671-UTR-E-siliculosus-1a_M_contig93:461610..4616271696251098.9973671-UTR-E-siliculosus-1a_M_contig93:461610..461627Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig93 461611..461627 -
1622936484.0635102-UTR-E-siliculosus-1a_M_contig93:463711..4638471622936484.0635102-UTR-E-siliculosus-1a_M_contig93:463711..463847Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig93 463712..463847 -
1696251099.0475776-UTR-E-siliculosus-1a_M_contig93:463711..4638471696251099.0475776-UTR-E-siliculosus-1a_M_contig93:463711..463847Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig93 463712..463847 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622936484.0071-CDS-E-siliculosus-1a_M_contig93:461627..4617531622936484.0071-CDS-E-siliculosus-1a_M_contig93:461627..461753Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig93 461628..461753 -
1696251099.0100107-CDS-E-siliculosus-1a_M_contig93:461627..4617531696251099.0100107-CDS-E-siliculosus-1a_M_contig93:461627..461753Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig93 461628..461753 -
1622936484.023313-CDS-E-siliculosus-1a_M_contig93:462502..4626011622936484.023313-CDS-E-siliculosus-1a_M_contig93:462502..462601Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig93 462503..462601 -
1696251099.0206945-CDS-E-siliculosus-1a_M_contig93:462502..4626011696251099.0206945-CDS-E-siliculosus-1a_M_contig93:462502..462601Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig93 462503..462601 -
1622936484.0403783-CDS-E-siliculosus-1a_M_contig93:463669..4637111622936484.0403783-CDS-E-siliculosus-1a_M_contig93:463669..463711Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig93 463670..463711 -
1696251099.0316322-CDS-E-siliculosus-1a_M_contig93:463669..4637111696251099.0316322-CDS-E-siliculosus-1a_M_contig93:463669..463711Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig93 463670..463711 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_M_contig93.17367.1

>prot_E-siliculosus-1a_M_contig93.17367.1 ID=prot_E-siliculosus-1a_M_contig93.17367.1|Name=mRNA_E-siliculosus-1a_M_contig93.17367.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=polypeptide|length=89bp
MHPPLLRPHPSCHEEVKVLMACHEENPYGKFFGACNDLKIALDSCFVHEK
EEKRRKNMAKARRFDAGFQKELELRRKELDQEQQQAGR*
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mRNA from alignment at E-siliculosus-1a_M_contig93:461611..463847-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_M_contig93.17367.1 ID=mRNA_E-siliculosus-1a_M_contig93.17367.1|Name=mRNA_E-siliculosus-1a_M_contig93.17367.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=mRNA|length=2237bp|location=Sequence derived from alignment at E-siliculosus-1a_M_contig93:461611..463847- (Ectocarpus siliculosus Ec864m_EcPH12_78m male)
ATCAGCTGCACTGAGCTGTGGAGACAAAGACACACACAGCCGACAGCAGG
CTAAGCTTTGCTCGTGGAAAATCTGCGCTCAGGATCGTTTGAGCTACACT
GGCTGACGTTGTTCGTGGATTTTATCACCGTGTACCATGCATCCGCCATT
GCTACGACCACACCCAAGCTGCCACGAGGTGAGGCGACCCTTTGCATTCG
TTTAAGCAGCACCGCAGGTTCCAAATCAGCTAACCGATCTTGACTCGCGC
AGACTCTACCGTGCACTGCGTACAGGCGTAGTAGTGCCTAGGTGTCTGTT
GCACGGGCGTGCTGTTGAGCCGGAGTCCAACCTGATGGGAACTTCCTTTG
AGATTCCACACCGAAGTGCGGGGTATCCTCGGAGGCACTGCGGTAGAGCC
AGCGCACGAGCTGCTGACGACTACAGTCAGCAGTTGTCACGGGCCGCCCC
GCCGGCCTCCTAGCGCAGTCCACCTGCCTAACGGTTGGCACGAGCAAGCA
CATATTTGTATTTCGTTACATAATGAAGTGGTCGATGTCATGCAGAGGCT
ACACTGCACTGCTAAACAGTGACACATGGTTGCTGCCGTCGACGGCGGCA
GTGTACATGTAGCTTACAACTCGCATGCAATACGCGTTTCGCTGCATTAT
TTGATTCGGTGTGACATTGCTCTGCGCATGGAAGTTCCAGGATGGCCTTT
CTAGACGCCGTACCACGTGGGAGTTTCCATCACAATGTGCCCTCGGGCAC
AATTTGCGTGCGTCACCACTCTTCGTGTACTCAGTAGCGTGAGGATGTGC
ATGAGCTCCTCGTTGCCGTCATTTCCCGCCAATTCCCGTTCAAGAGCCTT
AGACTCAGTGTGTTCCGAGACTCCGCGGTCTGACGACGTGAAATACGTCG
TCGTGAGAGGGAGGCGCCCCGCCCAAATCCTCCCCCCGTCCCATCGTCAC
AAACACACGTGTCGACGTGTTTGCGCTGCAGCGTAGGATAAGGATGATGC
AACGATGAGGAAGACTTCCACGTTCACAGAGCAGCAAGAATATGTGGTAC
ATTGGCAGCTCTGGGCGAAGAAAGGCCGGCGGTGCTCGCGCCCTCCCCTT
TTCCTTCATCGTCGGGCGCTGACTCAAAGAACAACGATTTGTTTACGATT
TGTTTGCCTACGCCCCTTCTTCCCTCTCGCACCCCGACGATGTACTTGCT
CACGGTATGGCTCCCTCGGTGCCCTGCAAATGTCGGCATCACCCAGGAGG
TGAAGGTGCTGATGGCGTGCCACGAGGAAAACCCGTACGGAAAGTTCTTC
GGGGCGTGCAACGACCTCAAGATCGCGCTCGATTCGTGCTTTGTGGTGAG
CAGCAGCAACAGTTGTAGATGGTCGGAGATGATAAAGAAAACAACAAACG
GAGAAAGCAGCACTCACCCACACTCGCGCGCAAGTTTTTCGAGGGCCCCC
GGGGGCCTGGAAATTTGACGTTGTTTGTCCCAGGACGACGTAGTGATGTT
TTCCTTCATATGTTGGCCTGGTGGCGACGTCGGTGCCATACAGACAAGCA
CACACGCACGCAAAGGTTGTTCGCGTTTGTCCTGCTATATACACGGACCA
CGTCTTGTCATTGGTGAGCTGTTTAGCGCGCTCGGTGCCAGGAACAGAAA
ACCACACACAGACGCACATGCATCTGCACGCAATCGCACATGCGCATGGC
ATTGCGAAGCATTTTTCTGTTTCAGCTGTACTGAGACTAACGTCTGTTCA
TTGGTGTTCATTGGTCAACCGGTTAGCGCGGTCGATATAGACACACCCCA
CACGCCCATGCTTCGTCCACAAAGCTATCCCCCGCCCCCCCCCGTGCCTT
CTACCCATCCGGCCCCTGCTCTCTGTCCTTCGTGTCGTGCCGCACATGCA
GATTGCACGTGCCTCAGAACCTCAAGCGTGCTGTATGGAAAAGGTTTTTG
TATCCGGTCTAGGTCAACGCCGATTTTTTTTGGAGTTTCCAGTCGTTGGC
TTTCCCTACAAGGATCAATTCCTCTTTTGTGTTCAACATTGGGGCCATCA
TCCCCTCTCTGCCTCTACACATGTTCCGATTTCCATGCACGCAGCACGAA
AAAGAGGAGAAGCGTCGGAAGAACATGGCCAAGGCCCGAAGGTTCGACGC
CGGCTTTCAGAAGGAGCTCGAACTCAGGCGAAAGGAGCTGGATCAGGAAC
AACAGCAGGCGGGACGGTAGCGGCAGTGGTGGTGTTG
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Coding sequence (CDS) from alignment at E-siliculosus-1a_M_contig93:461611..463847-

>mRNA_E-siliculosus-1a_M_contig93.17367.1 ID=mRNA_E-siliculosus-1a_M_contig93.17367.1|Name=mRNA_E-siliculosus-1a_M_contig93.17367.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=CDS|length=534bp|location=Sequence derived from alignment at E-siliculosus-1a_M_contig93:461611..463847- (Ectocarpus siliculosus Ec864m_EcPH12_78m male)
ATGCATCCGCCATTGCTACGACCACACCCAAGCTGCCACGAGATGCATCC
GCCATTGCTACGACCACACCCAAGCTGCCACGAGGAGGTGAAGGTGCTGA
TGGCGTGCCACGAGGAAAACCCGTACGGAAAGTTCTTCGGGGCGTGCAAC
GACCTCAAGATCGCGCTCGATTCGTGCTTTGTGGAGGTGAAGGTGCTGAT
GGCGTGCCACGAGGAAAACCCGTACGGAAAGTTCTTCGGGGCGTGCAACG
ACCTCAAGATCGCGCTCGATTCGTGCTTTGTGCACGAAAAAGAGGAGAAG
CGTCGGAAGAACATGGCCAAGGCCCGAAGGTTCGACGCCGGCTTTCAGAA
GGAGCTCGAACTCAGGCGAAAGGAGCTGGATCAGGAACAACAGCAGGCGG
GACGGTAGCACGAAAAAGAGGAGAAGCGTCGGAAGAACATGGCCAAGGCC
CGAAGGTTCGACGCCGGCTTTCAGAAGGAGCTCGAACTCAGGCGAAAGGA
GCTGGATCAGGAACAACAGCAGGCGGGACGGTAG
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