mRNA_E-siliculosus-1a_M_contig9.17024.1 (mRNA) Ectocarpus siliculosus Ec864m_EcPH12_78m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_E-siliculosus-1a_M_contig9.17024.1
Unique Name	mRNA_E-siliculosus-1a_M_contig9.17024.1
Type	mRNA
Organism	Ectocarpus siliculosus Ec864m_EcPH12_78m male (Ectocarpus siliculosus Ec864m_EcPH12_78m male)

Homology

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BLAST of mRNA_E-siliculosus-1a_M_contig9.17024.1 vs. uniprot
Match: D7G1G1_ECTSI (Mannuronan C-5-epimerase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G1G1_ECTSI)

HSP 1 Score: 743 bits (1918), Expect = 2.760e-261
Identity = 391/493 (79.31%), Postives = 435/493 (88.24%), Query Frame = 2

Query:  185 GLGGSGVLLCFVVIGTMVGTASAGSIRMTECPPDAPEATFRWVNEGEHLYVEGAGSCVTLPDIYDAVENPPLTYHTVEGDESDTETGIWLMTKNIFMESGTTLLLHDDLGCDELRIISSSTNARHLRAFGAYISLLRTKLTSWSVGNRAPDNGAYDGLNARSFISAVSEMTHPSDTCDGAADDDAGEARMDIINSEVAYLGYQASESYGISYKVRGYCTDESNPEVFDRVNVRGDIRFSDIHHNWFGHYSYGHQDGLWEYNTMHNCGQYGFDPHDDSDNLRIHNNIVYENGNHGIIASKRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGSGSACIAIFESFDIEISDNLCTENEEGIRLSMGSSYNKIFDNQVIDTAGRSLWMYLGSDEVEASAATDGMCTGNLFQNNYLEGSETGVAMTETRDTMLIGNTFIDTDNNEWKDSDGLVWRDNTIDGDFDMRFSDTCVTSESDFDQINGVGSNPSEC 1663
            G   +G+LL  ++ GT V T SAGSIR+TECP DAPEATF W N+G++LYVEG GSCVTLPDIY+ V+NPPLTYHTV+GDESDTETGIWL+TK+++M  GTTLLLHDDLGC ELRI SSSTNA HL  FG YIS+L TK+TSWSV N+ PD GAYDG N RS++SAVSEMTHPSDTCDGAADDDAGEAR+DIINS+V YLGYQA+ESYG++YKVRGYCTDESNPEVFDRVNVRGDIRFSDIHHNWFG YSYGHQDGLWEYNTM N G YGFDPHDDSDNLRIHNNIVYENGNHGIIASKR XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX +GSGSACIAIFESF IEISDN+CT+NEEGIRLSMGSSYNKIFDNQVIDTAGRSLW+YLGSDE+ ASAATDGMCTGNLFQNNYL+G+  GV  +ETRD MLIGNT +  + +   DSDGL+W DN ++ DF++    TC++SESDFDQI+  GSNPSEC
Sbjct:    9 GSAAAGLLLFLLLAGTRVQTGSAGSIRLTECPADAPEATFSWTNDGDYLYVEGDGSCVTLPDIYNGVDNPPLTYHTVDGDESDTETGIWLLTKHLYMRPGTTLLLHDDLGCTELRIASSSTNALHLNTFGGYISILNTKVTSWSVSNKEPDAGAYDGSNPRSYMSAVSEMTHPSDTCDGAADDDAGEARLDIINSDVGYLGYQAAESYGLTYKVRGYCTDESNPEVFDRVNVRGDIRFSDIHHNWFGQYSYGHQDGLWEYNTMRNSGYYGFDPHDDSDNLRIHNNIVYENGNHGIIASKRCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVTGSGSACIAIFESFGIEISDNVCTDNEEGIRLSMGSSYNKIFDNQVIDTAGRSLWLYLGSDEIIASAATDGMCTGNLFQNNYLDGASIGVKFSETRDNMLIGNTMVGVEESLIDDSDGLLWSDNEVEDDFEIGIETTCLSSESDFDQISAGGSNPSEC 501

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BLAST of mRNA_E-siliculosus-1a_M_contig9.17024.1 vs. uniprot
Match: D7G1G2_ECTSI (Mannuronan C-5-epimerase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G1G2_ECTSI)

HSP 1 Score: 625 bits (1611), Expect = 4.990e-217
Identity = 351/406 (86.45%), Postives = 353/406 (86.95%), Query Frame = 2

Query:  446 IWLMTKNIFMESGTTLLLHDDLGCDELRIISSSTNARHLRAFGAYISLLRTKLTSWSVGNRAPDNGAYDGLNARSFISAVSEMTHPSDTCDGAADDDAGEARMDIINSEVAYLGYQASESYGISYKVRGYCTDESNPEVFDRVNVRGDIRFSDIHHNWFGHYSYGHQDGLWEYNTMHNCGQYGFDPHDDSDNLRIHNNIVYENGNHGIIASKRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGSGSACIAIFESFDIEISDNLCTENEEGIRLSMGSSYNKIFDNQVIDTAGRSLWMYLGSDEVEASAATDGMCTGNLFQNNYLEGSETGVAMTETRDTMLIGNTFIDTDNNEWKDSDGLVWRDNTIDGDFDMRFSDTCVTSESDFDQINGVGSNPSEC 1663
            IWLMTKNIFMESGTTLLLHDDLGCD                           LTSWSVGNRAPDNGAYDGLNARSFISAVSEMTHPSDTCDGAADDDAGEARMDIINSEVAYLGYQASESYGISYKVRGYCTDESNPEVFDRVNVRGDIRFSDIHHNWFG                    QYGFDPHDDSDNLRIHNNIVYENGNHGIIASKR     XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGSGSACIAIFESFDIEISDNLCT+NEEGIRLSMGSSYNKIFDNQVIDTAGRS+WMYLGSDEVEASAATDGMCTGNLFQNNYLEGSETGVAMTETRDTMLIGNTFIDTDNNEWKDSDGLVWRDN IDGDFDMRFSDTCVTSESDFDQINGVGSNPSEC
Sbjct:    3 IWLMTKNIFMESGTTLLLHDDLGCD---------------------------LTSWSVGNRAPDNGAYDGLNARSFISAVSEMTHPSDTCDGAADDDAGEARMDIINSEVAYLGYQASESYGISYKVRGYCTDESNPEVFDRVNVRGDIRFSDIHHNWFGQ-------------------QYGFDPHDDSDNLRIHNNIVYENGNHGIIASKRCNDVSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGSGSACIAIFESFDIEISDNLCTQNEEGIRLSMGSSYNKIFDNQVIDTAGRSIWMYLGSDEVEASAATDGMCTGNLFQNNYLEGSETGVAMTETRDTMLIGNTFIDTDNNEWKDSDGLVWRDNNIDGDFDMRFSDTCVTSESDFDQINGVGSNPSEC 362

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BLAST of mRNA_E-siliculosus-1a_M_contig9.17024.1 vs. uniprot
Match: A0A6H5K9I9_9PHAE (MEP6 protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K9I9_9PHAE)

HSP 1 Score: 325 bits (833), Expect = 1.470e-98
Identity = 230/490 (46.94%), Postives = 308/490 (62.86%), Query Frame = 2

Query:  224 IGTMVGTASAGSIRMTECPPDAPEA--TFRWVNEGEHLYVEGAGSCVTLPDIY-----DAVENP------------PLTYHTVEGDESDTETGIWLMTKNIFMESGTTLLLHDDLGCDELRIISSSTNARHLRAFGAYISLLRTKLTSWSVGNRAPDNGAYDGLNARSFISAVSE-MTHPSDTCDGAADDDAGEARMDIINSEVAYLGYQASESYGISYKVRGYCTDESNPEVFDRVNVRGDIRFSDIHHNWFGHYSYGHQDGLWEYNTMHNCGQYGFDPHDDSDNLRIHNNIVYENGNHGIIASKRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGSGSACIAIFESFDIEISDNLCTENEEGIRLSMGSSYNKIFDNQVIDTAGRSLWMYLGSDEVEASAATDGMCTGNLFQNNYLEGSETGVAMTETRDTMLIGNTFIDTDNNEWKDSDGLVWRDNTIDGDFDMRFSDTCVTSESDFDQI 1633
            +G +   A+AG   + E      EA  + R+  E + LY+EG G C+T  DIY     +  E+             P+T  T +G+ SD ETG WL T ++++  G  L LH + G   +R+ S S     LRA G ++SLL T+L+SW V + A     Y     RS+++A+SE +  P  TC G A +DAGEAR DIINSEV +LG+  +E+YGI++KVRG+C D SN ++FDRVNVRGDIRFSDIHHN+FG Y+YGHQDGLWEYN MH   +YGFDPHDDSDNLRIHNNIV+            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX        AIFES + + S+N CT+N+ G R S+G+S+NKI+DN+V D+    ++MY GSD    S   DG+ + N+  NN ++ +  G+ M +T      GN   +T +  +++S  ++W+DN I    D+    +C TS+SD  Q+
Sbjct:   11 VGLVAAAAAAGLATLGEACDYTLEAKASLRFNIETDRLYLEGGG-CITPSDIYLAKLTNVTEDDGNTLTAEEAAAIPITPITKDGEFSDAETGYWLFTSDVYIYDGVKLELHAESGTTGMRLKSDS-KVIELRAHGGWLSLLNTELSSWDV-DEADYASNYSA--PRSYVTAISEVLDSPPQTCVGHAKNDAGEARFDIINSEVHHLGHYGTEAYGITWKVRGFCVDLSNEDLFDRVNVRGDIRFSDIHHNYFGMYTYGHQDGLWEYNLMHENAKYGFDPHDDSDNLRIHNNIVWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAIFESMEGDYSNNKCTDNKYGFRWSVGASHNKIYDNEVYDSQQYGIYMYEGSDLPYVS---DGLPSYNIIANNIIDTATEGIKMGDTVGNEFTGNVITNTADLRFRNSSEVIWKDNDIPAGVDIDSEGSCFTSDSDIVQL 492

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BLAST of mRNA_E-siliculosus-1a_M_contig9.17024.1 vs. uniprot
Match: D7FXE4_ECTSI (Mannuronan C-5-epimerase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FXE4_ECTSI)

HSP 1 Score: 300 bits (767), Expect = 9.080e-89
Identity = 188/480 (39.17%), Postives = 265/480 (55.21%), Query Frame = 2

Query:  260 IRMTECPPDAPEATFRWVNEGEHLYVEGA-----GSCVTLPDIYD--AVENPPLTYHTVEGDESDTETGIWLMTKNIFMESGTTLLLH--DDLG-CDELRIISSSTNARHLRAFGAYISLLRTKLTSWSVGNRAPDNGAYDGLNARSFISAVSEM-THPSDTCDGAADDDAGEARMDIINSEVAYLGYQASESYGISYKVRGYCTDESNPEVFDRVNVRGDIRFSDIHHNWFGHYSYGHQDGLWEYNTMHNCGQYGFDPHDDSDNLRIHNNIVYENGNHGIIASKRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--SGSGSACIAIFESFDIEISDNLCTENEEGIRLSMGSSYNKIFDNQVIDTAGRSLWMYLGSDEVEASAATDGMCTGNLFQNNYLEGSETGVAMTETRDTMLIGNTFIDTDNNEWKDSDGLVWR-DNTI-----------DGDFDMRFS-DTCVTSESD 1621
            I   +C  D  E + R+    + LY+E A     G C TL  I++  A + P        GD S+TETG WL+T+ +++E G TL ++  D+ G  DELR++S+S    +LRA G  +  + TK+ SW     +PD    DG   RS+ISAVSE+ T   +TC+G A +  GEARMDI NSE+ YLG+Q SESYG+++KVRG+C D+SNPE+FD VNV G++  SDIHH  FG Y+YGHQ G W  N MH+   YGFDPHDDSD L IH+N VY NG HGIIASKR                                       +G A +A+ ESF+ ++S+N    N+ G+R+S+G + N   +N + + +  + + YLGSDE +    + G    N+F  N   G+E  + + E   T  + N F      E  D+DGLV R D+ +           +GDF+++ + D+C   ESD
Sbjct:   38 ISKPDCDSDI-EVSIRYAGTTKRLYLESADGETRGGCATLGQIWESRAGKAPLYAVDPDSGDVSETETGTWLLTEELYVEDGITLKVYGTDEGGDADELRLLSTSETYINLRAHGGSLDFVGTKVFSWDTSTNSPDEDETDG---RSYISAVSEIVTDEDETCEGNAKNTMGEARMDIENSEIGYLGFQDSESYGLTWKVRGFCKDKSNPEIFDDVNVYGNMYDSDIHHLRFGLYTYGHQQGDWRRNKMHDNSGYGFDPHDDSDFLTIHDNEVYNNGYHGIIASKRCNGVSIQGNEVYGGAETSAGIFLHRSSDDAIVKGNYVHDNGDAGLAMLESFNADVSENTFENNKYGVRMSVGCADNVFSENIISNNSKYNAYTYLGSDEPDV--VSSGRSQNNVFSQNSFSGAEETIKIKEADGTQFLDNVF------EVGDADGLVVRFDDAMNSVMQGNTGLDEGDFELKVTNDSCFDGESD 505

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BLAST of mRNA_E-siliculosus-1a_M_contig9.17024.1 vs. uniprot
Match: Q7XAJ7_9PHAE (Mannuronan C-5-epimerase n=1 Tax=Laminaria digitata TaxID=80365 RepID=Q7XAJ7_9PHAE)

HSP 1 Score: 292 bits (747), Expect = 5.040e-86
Identity = 193/478 (40.38%), Postives = 263/478 (55.02%), Query Frame = 2

Query:  260 IRMTECPPDAPEATFRWVNEGEHLYVEGA-----GSCVTLPDIYDAV--ENPPLTYHTVEGDESDTETGIWLMTKNIFMESGTTLLLHDDLG---CDELRIISSSTNARHLRAFGAYISLLRTKLTSWSVGNRAPDNGAYDGLNARSFISAVSEM-THPSDTCDGAADDDAGEARMDIINSEVAYLGYQASESYGISYKVRGYCTDESNPEVFDRVNVRGDIRFSDIHHNWFGHYSYGHQDGLWEYNTMHNCGQYGFDPHDDSDNLRIHNNIVYENGNHGIIASKRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGSG--SACIAIFESFDIEISDNLCTENEEGIRLSMGSSYNKIFDNQVIDTAGRSLWMYLGSDEVEASAATDGMCTGNLFQNNYLEGSETGVAMTETRDTMLIGNTFIDTDNNEWKDSDGLVWRDNTIDGDFDMRFSDTCVTSESDFDQINGVGSNP 1654
            I   EC  ++   + R+ +  E LY+E A     G CVTL  I++    E P     +  GD SD+ TG WL+T+ +F+E G TL +H        DELR++S+S    +LRA G  +  L TK+ +W      PD    DG   RS++SAVSE+ T  S TC+G A ++ GEARMDI +SE+ YLGY  SESYG+++KVRG+C D+SN ++F+ VNV G+I  SDIHHN FG Y+YGHQ G W  N MH+   YGFDPHDDSD L IH+N VY+NG HGIIASKR                         XXXXXXXXXX       A +A+ ESF+ ++SDN+   N+ GIR S+GS  N  F+N +  +   S + YLGSD  +      G    N+FQ N + G    + + +   T  I NTF D     + D+   +     + G+  +  S   V + + FD  +  G  P
Sbjct:   31 IPKPECD-ESIAVSIRYSSVYERLYLESADSKTRGGCVTLEQIWEHQRGEAPLYAVDSKSGDISDSATGTWLLTEELFVEDGITLKVHGTSAGGDADELRLLSTSDTFINLRAHGGSLDFLSTKVFAWDTSINGPDRDEEDG---RSYVSAVSEVITDDSQTCNGRAKNNMGEARMDIEDSEMGYLGYHGSESYGLTWKVRGFCVDKSNHDLFEDVNVYGNIYDSDIHHNNFGVYTYGHQQGDWRRNKMHDNSGYGFDPHDDSDFLTIHDNEVYDNGYHGIIASKRCNGVSIQGNEVYGGATSSAGIFLHRXXXXXXXXXXXXXXXXDAGLAMMESFNADVSDNIFENNKYGIRFSVGSGRNVFFNNVISGSTRYSTYSYLGSDAPDV--VDSGRSQDNVFQENNIIGGVESIKLLDADGTQFIDNTFEDATTIRFNDAKKTL-----MSGNIGLNMSKLKVVNGASFDASSDYGFEP 497

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BLAST of mRNA_E-siliculosus-1a_M_contig9.17024.1 vs. uniprot
Match: Q7XAJ8_9PHAE (Mannuronan C-5-epimerase (Fragment) n=1 Tax=Laminaria digitata TaxID=80365 RepID=Q7XAJ8_9PHAE)

HSP 1 Score: 290 bits (742), Expect = 1.280e-85
Identity = 173/417 (41.49%), Postives = 231/417 (55.40%), Query Frame = 2

Query:  299 TFRWVNEGEHLYVEGA-----GSCVTLPDIYDA-VENPPL-TYHTVEGDESDTETGIWLMTKNIFMESGTTLLLHDDLG---CDELRIISSSTNARHLRAFGAYISLLRTKLTSWSVGNRAPDNGAYDGLNARSFISAVSEM-THPSDTCDGAADDDAGEARMDIINSEVAYLGYQASESYGISYKVRGYCTDESNPEVFDRVNVRGDIRFSDIHHNWFGHYSYGHQDGLWEYNTMHNCGQYGFDPHDDSDNLRIHNNIVYENGNHGIIASKRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--SGSGSACIAIFESFDIEISDNLCTENEEGIRLSMGSSYNKIFDNQVIDTAGRSLWMYLGSDEVEASAATDGMCTGNLFQNNYLEGSETGVAMTETRDTMLIGNTFID 1510
            + R+  E   LY+E A     G CVTL  I++  V+  PL       GD S + TG WL+T+ +++E G TL +H        DELR++S+S    HLRA+G  +  L TK+ +W     APD    DG   RS+ISAVSE+ T    TC+G A ++ GEARMDI NSE+AYLGY  +E+YG+++KVRG+C D+ NPE+FD VNV G+I  SDIHHN FG Y+YGHQ G W  N MH+   YGFDPHDDSD L IH+N V+ NG HGIIASKR                                       +G A +A+ ESF+ ++SDN    N+ G+RLS+G   N    N +  +A  + + YLG+D+ E      G    N+F  N + G    + + E   T  I NTF D
Sbjct:   17 SVRYNAESARLYIESANGRTRGGCVTLEQIWEQQVDGSPLYAVDPKSGDVSRSATGTWLLTEEMYVEDGITLQVHGISAGGDADELRLLSTSDTFIHLRAYGGSLDFLSTKVFAWDTSKNAPDQDESDG---RSYISAVSEVITDDRQTCNGRAKNNMGEARMDIENSEMAYLGYHGTETYGLTWKVRGFCVDKGNPELFDDVNVYGNIYDSDIHHNNFGVYTYGHQQGDWRRNKMHDNSGYGFDPHDDSDFLTIHDNEVWNNGYHGIIASKRCNGVSIQGNEVYGGSSGSAGIFLHRSSDNGIVKGNYVHDNGDAGLAMLESFNADVSDNTFENNKYGVRLSVGCGRNIFSKNVITGSAEFNTFSYLGADKPEV--VDSGRSQDNVFMENNIIGGVESILLKEADGTQFIDNTFKD 428

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BLAST of mRNA_E-siliculosus-1a_M_contig9.17024.1 vs. uniprot
Match: A0A6H5LHR7_9PHAE (MEP7d protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5LHR7_9PHAE)

HSP 1 Score: 280 bits (717), Expect = 3.920e-85
Identity = 168/221 (76.02%), Postives = 175/221 (79.19%), Query Frame = 2

Query: 1055 GNHGIIASKRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGSGSACIAIFESFDIEISDNLCTENEEGIRLSMGSSYNKIFDNQVIDTAGR------------------SLWMYLGSDEVEASAATDGMCTGNLFQNNYLEGSETGVAMTETRDTMLIGNTFIDTDNNEWKDSDGLVWRDNTIDGDFDMRFSDTCVTSESDFDQINGVGSNPSEC 1663
            GNHGIIASKR                XXXXXXXXXXXXXXXXXXXSGSGSACIAIFESFDIEISDNLCT NEEGIRLSMGSSYNKIFDNQVIDTAGR                  ++WMYLGSDEVEASAATDGMCTGNLFQNNYLE S+TGV++TETRDTMLIGNTFIDTDNNEW+D        N IDGDFDM FSDTCVTS+SDFDQINGVGSNPSEC
Sbjct:   21 GNHGIIASKRCNDVSIQNNVVYDNALXXXXXXXXXXXXXXXXXXXSGSGSACIAIFESFDIEISDNLCTNNEEGIRLSMGSSYNKIFDNQVIDTAGREFCYHIMPQCGWLTYPRGAIWMYLGSDEVEASAATDGMCTGNLFQNNYLERSDTGVSLTETRDTMLIGNTFIDTDNNEWRDXXXXXXXXNDIDGDFDMSFSDTCVTSDSDFDQINGVGSNPSEC 241

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BLAST of mRNA_E-siliculosus-1a_M_contig9.17024.1 vs. uniprot
Match: D7G339_ECTSI (Mannuronan C-5-epimerase n=2 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G339_ECTSI)

HSP 1 Score: 285 bits (730), Expect = 1.870e-83
Identity = 179/468 (38.25%), Postives = 253/468 (54.06%), Query Frame = 2

Query:  299 TFRWVNEGEHLYVEGA------GSCVTLPDIYD--AVENPPLTYHTVEGDESDTETGIWLMTKNIFMESGTTLLLH-DDLG--CDELRIISSSTNARHLRAFGAYISLLRTKLTSWSVGNRAPDNGAYDGLNARSFISAVSEM-THPSDTCDGAADDDAGEARMDIINSEVAYLGYQASESYGISYKVRGYCTDESNPEVFDRVNVRGDIRFSDIHHNWFGHYSYGHQDGLWEYNTMHNCGQYGFDPHDDSDNLRIHNNIVYENGNHGIIASKRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--SGSGSACIAIFESFDIEISDNLCTENEEGIRLSMGSSYNKIFDNQVIDTAGRSLWMYLGSDEVEASAATDGMCTGNLFQNNYLEGSETGVAMTETRDTMLIGNTFIDTDNNEWKDSDGLVWR-DNTI-----------DGDFDMRF-SDTCVTSESD 1621
            + R+      LY+E A      G CVTL  I++  A + P        G+ S + TG WL+T+ +++E G TL ++  + G   DELR++S++    +LRA G  +  + TK+ SW     +PD    DG   RS+ISA+SE+ T   +TC+G A +  GEARMDI +SE+ YLGY  SESYG+++KVRG+C D+SNPEVFD VNV G++  SDIHH  FG Y+YGHQ G W  N MH+   YGFDPHDDSD L IH+N VY NG HGIIASKR                                       +G A +A+ ESF+ ++S+N    N+ G+R S+G + N   +N + + A  + + YLG+DE +    + G    N+F  N   G+E  + + E   T  + N F      E  D+DGLV R DN             DG+F+++  +D C   E+D
Sbjct:   45 SIRYAGTTARLYLESADNGTTRGGCVTLGQIWESRAGKGPLYAVDPDSGNVSTSATGTWLLTEELYVEDGITLKVYGSEAGGDADELRLLSTNETFINLRAHGGSLDFVSTKVFSWDTSTNSPDEDETDG---RSYISAISEIITDEDETCEGNAKNTMGEARMDIESSEIGYLGYWDSESYGLTWKVRGFCEDKSNPEVFDDVNVYGNVYDSDIHHMNFGLYTYGHQQGDWRRNKMHDNSGYGFDPHDDSDFLTIHDNEVYNNGYHGIIASKRCNGVSIQGNEVYGGAETSAGIFLHRSSDDAIVKGNYVHDNGDAGLAMLESFNADVSENTFENNKYGVRFSVGCADNVFSNNTISNNAEYNAYSYLGTDEPDV--VSSGRSQNNVFSQNSFSGAEETIKIKEADGTQFLDNVF------EVGDADGLVVRFDNATENLMQGNTGLDDGEFELKVDNDACFDGEAD 501

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BLAST of mRNA_E-siliculosus-1a_M_contig9.17024.1 vs. uniprot
Match: D8LC73_ECTSI (Mannuronan C-5-epimerase 4 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LC73_ECTSI)

HSP 1 Score: 277 bits (708), Expect = 8.680e-77
Identity = 180/481 (37.42%), Postives = 245/481 (50.94%), Query Frame = 2

Query:  269 TECPPDAPEATFRWVNEGE---HLYVEGAGSCVTLPDIY-------DAVENPPLTYHTVEGDESDTETGI---WLMTKNIFMESGTTLLLHDDLG---CDELRIISSSTNARH-LRAFGAYISLLRTKLTSWSVGNRAPDNGAYDGLNARSFISAVSEMTHPSDTCDGAADDDAGEARMDIINSEVAYLGYQASESYGISYKVRGYCTDESNPEVFDRVNVRGDIRFSDIHHNWFGHYSYGHQDGLWEYNTMHNCGQYGFDPHDDSDNLRIHNNIVYENGNHGIIASKRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGSG--SACIAIFESFDIEISDNLCTENEEGIRLSMGSSYNKIFDNQVIDTAGRSLWMYLGSDEVEASAATDGMCTGNLFQNNYLEGSETGVAMTETRDTMLIGNTFIDTDNNEWKDSDGLVWRDNTIDGDFDMRFSDTCVTSESDFDQINGVGSNP 1654
            + C  D    +FR+         LY EG G C T+ D+Y       D     P+     +G + D  +GI   WL+   +++  G             C++LRI S+ ++  H +R  G  +    T +TSW   N+ P    Y+  N RSF++ VSE     D C+G A ++ GE RMDIINSE+AYLGY  SESYG+++KVRG+C D SNPEVF+  NV GD+  SDIHH ++GHYSYGHQ G+W  N MH+  QYGFDPHDDSD L I  N VY N NHGIIASKR                                   + +    A IA+ ESFD +I  N+    + GIR+S+GS  N+++ N   D +   L+ Y GSDE + S   DG  +GN+   N +E    GV + E  D     NTFI+ D  E+ +S   +W  N I         D C+  + +FD  +G  S P
Sbjct:  419 SSCGDDEDRPSFRYAASSGVAGRLYAEGEG-CFTMTDLYNFRGTENDGSSKGPVYMLDDDGTKLDIPSGITNNWLLAAELYITGGGVFYCKGTSAGGDCNQLRIQSTGSDDFHEVRGHGGSLYFEDTIVTSWDTPNKEPQE-TYE--NGRSFLNCVSEKL-TGDDCEGNAKNEMGECRMDIINSEIAYLGYFDSESYGMTWKVRGFCKDLSNPEVFELTNVYGDLTGSDIHHLYYGHYSYGHQGGVWTNNKMHDNWQYGFDPHDDSDYLTIAYNEVYNNVNHGIIASKRCNNVKIFENEVRDGGAAAAGIFLHRSSDGAAVFDNTITNMQDAGIAMLESFDAQIYGNIINGAKYGIRMSLGSGNNEVYQNSFTDISQYGLYTYEGSDEPDVS---DGRPSGNVLALNLVEDCPIGVKIKEGDDNEFYQNTFINVDAFEFPESTNTMWSGNDI--------GDGCIEEDDEFDAASGSDSIP 883

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BLAST of mRNA_E-siliculosus-1a_M_contig9.17024.1 vs. uniprot
Match: A8CEP1_SACJA (Mannuronan C-5 epimerase n=1 Tax=Saccharina japonica TaxID=88149 RepID=A8CEP1_SACJA)

HSP 1 Score: 266 bits (681), Expect = 1.670e-76
Identity = 183/477 (38.36%), Postives = 257/477 (53.88%), Query Frame = 2

Query:  242 TASAGSIRMTECPPDAPEATFRWVNEGEHLYVEGA-----GSCVTLPDIYDAVEN--PPLTYHTVEGDESDTETGIWLMTKNIFMESGTTLLLHDDLG---CDELRIISSSTNARHLRAFGAYISLLRTKLTSWSVGNRAPDNGAYDGLNARSFISAVSEM-THPSDTCDGAADDDAGEARMDIINSEVAYLGYQASESYGISYKVRGYCTDESNPEVFDRVNVRGDIRFSDIHHNWFGHYSYGHQDGLWEYNTMHNCGQYGFDPHDDSDNLRIHNNIVYENGNHGIIASKRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--SGSGSACIAIFESFDIEISDNLCTENEEGIRLSMGSSYNKIFDNQVIDTAGRSLWMYLGSDE---VEASAATDGMCTGNLFQNNYLEGSETGVAMTETRDTMLIGNTFIDTDNNEWKDSDGLVWRDNTIDGDFDMRFSD-TCVTSESD 1621
            TAS            + + + R+    + LY+E A     G CVTL DI+  ++   P        GD S + TG WL+T+++++E G TL +H        DELR++S+S +  +LRA G  +  + TK+ +W            D    RS+ISAVSE+ T     CDG A  + GEARMDI +SE+AYLGY  SESYG+++KVRG C D SN EVFD VNV G+I  SDIHHN+FG Y+YGH+ G W  N MH+  +YGFDPHDDSD L IH+N VY NGNHGIIASKR   XXXXXXXXXX                          +G A +A+ E+F+ ++S+N   +N+ GIR+S+G + N    N +  ++  +++ Y GSD    V++  + D              G +  V +T    T  I NTF       ++D  G +   N+  G   ++ +D +C   +SD
Sbjct:   24 TASRNKAIAKPSCKSSTKVSIRYSKTSKRLYLESADGNTRGGCVTLEDIWRDLDGGAPLYAVKPSSGDVSSSITGTWLLTESLYVEDGITLQVHGKSAGGKADELRLLSTSKDFINLRAHGGSLDFVSTKVFAWDTSKNKVRT---DDSKGRSYISAVSEVITDSKQKCDGRAKKNMGEARMDIEDSEMAYLGYFGSESYGLTWKVRGLCRDLSNLEVFDAVNVYGNIYDSDIHHNYFGVYTYGHEQGDWRRNKMHHNTEYGFDPHDDSDFLTIHDNEVYNNGNHGIIASKRCNGXXXXXXXXXXGAAKSVGIFLHRSSDDAIVKDNYVHDNGDAGMALMETFNADVSNNKFEDNKYGIRMSVGCADNVFSKNDITGSSKYNVYTYQGSDAPWVVDSGRSQD-----XXXXXXXXSGGQETVKLTMADGTEFIDNTFKSAKTIRFEDCQGTLMSGNSGLGGASLKVTDGSCFDKKSD 492

The following BLAST results are available for this feature:

BLAST of mRNA_E-siliculosus-1a_M_contig9.17024.1 vs. uniprot

BLAST of mRNA_E-siliculosus-1a_M_contig9.17024.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a male vs UniRef90)
Total hits: 25


Match Name	E-value	Identity	Description
D7G1G1_ECTSI	2.760e-261	79.31	Mannuronan C-5-epimerase n=1 Tax=Ectocarpus silicu...	[more]
D7G1G2_ECTSI	4.990e-217	86.45	Mannuronan C-5-epimerase n=1 Tax=Ectocarpus silicu...	[more]
A0A6H5K9I9_9PHAE	1.470e-98	46.94	MEP6 protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 T...	[more]
D7FXE4_ECTSI	9.080e-89	39.17	Mannuronan C-5-epimerase n=1 Tax=Ectocarpus silicu...	[more]
Q7XAJ7_9PHAE	5.040e-86	40.38	Mannuronan C-5-epimerase n=1 Tax=Laminaria digitat...	[more]
Q7XAJ8_9PHAE	1.280e-85	41.49	Mannuronan C-5-epimerase (Fragment) n=1 Tax=Lamina...	[more]
A0A6H5LHR7_9PHAE	3.920e-85	76.02	MEP7d protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 ...	[more]
D7G339_ECTSI	1.870e-83	38.25	Mannuronan C-5-epimerase n=2 Tax=Ectocarpus silicu...	[more]
D8LC73_ECTSI	8.680e-77	37.42	Mannuronan C-5-epimerase 4 n=1 Tax=Ectocarpus sili...	[more]
A8CEP1_SACJA	1.670e-76	38.36	Mannuronan C-5 epimerase n=1 Tax=Saccharina japoni...	[more]

Pages

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
E-siliculosus-1a_M_contig9	contig	E-siliculosus-1a_M_contig9:1189738..1201889 -

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a male vs UniRef90	2022-09-19
OGS1.0 of Ectocarpus siliculosus Ec864m_EcPH12_78m male	2021-02-24

Properties


Property Name	Value
Start	1
Stop	1
Cds size	1527
Model size	2517
Exons	13
Hectar predicted targeting category	signal peptide
Seed eggNOG ortholog	2880.D8LC73
Seed ortholog evalue	1.7e-72
Seed ortholog score	280.4
Taxonomic scope	Eukaryota
BRITE	ko00000,ko00001
Best eggNOG OG	NA\|NA\|NA
Best tax level	Eukaryota
COG Functional cat.	U
Ec32 ortholog	Ec-07_000900.1
Ec32 ortholog description	Mannuronan C-5-epimerase C-terminal fragment
EggNOG OGs	KOG4157@1,KOG4157@2759
EggNOG free text desc.	protein xylosyltransferase activity
KEGG Pathway	ko04011,ko04139,map04011,map04139
KEGG ko	ko:K11244

Relationships

The following UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1622936397.2674754-UTR-E-siliculosus-1a_M_contig9:1189737..1190588	1622936397.2674754-UTR-E-siliculosus-1a_M_contig9:1189737..1190588	Ectocarpus siliculosus Ec864m_EcPH12_78m male	UTR	E-siliculosus-1a_M_contig9 1189738..1190588 -
1696251059.634006-UTR-E-siliculosus-1a_M_contig9:1189737..1190588	1696251059.634006-UTR-E-siliculosus-1a_M_contig9:1189737..1190588	Ectocarpus siliculosus Ec864m_EcPH12_78m male	UTR	E-siliculosus-1a_M_contig9 1189738..1190588 -
1622936397.7035112-UTR-E-siliculosus-1a_M_contig9:1201750..1201889	1622936397.7035112-UTR-E-siliculosus-1a_M_contig9:1201750..1201889	Ectocarpus siliculosus Ec864m_EcPH12_78m male	UTR	E-siliculosus-1a_M_contig9 1201751..1201889 -
1696251059.8372278-UTR-E-siliculosus-1a_M_contig9:1201750..1201889	1696251059.8372278-UTR-E-siliculosus-1a_M_contig9:1201750..1201889	Ectocarpus siliculosus Ec864m_EcPH12_78m male	UTR	E-siliculosus-1a_M_contig9 1201751..1201889 -

The following CDS feature(s) are a part of this mRNA:

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_E-siliculosus-1a_M_contig9.17024.1	prot_E-siliculosus-1a_M_contig9.17024.1	Ectocarpus siliculosus Ec864m_EcPH12_78m male	polypeptide	E-siliculosus-1a_M_contig9 1190589..1201750 -

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at E-siliculosus-1a_M_contig9:1189738..1201889-
coding sequence from alignment at E-siliculosus-1a_M_contig9:1189738..1201889-

protein sequence of mRNA_E-siliculosus-1a_M_contig9.17024.1

>prot_E-siliculosus-1a_M_contig9.17024.1 ID=prot_E-siliculosus-1a_M_contig9.17024.1|Name=mRNA_E-siliculosus-1a_M_contig9.17024.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=polypeptide|length=509bp
MRSSKMDRRRRAASGGLGGSGVLLCFVVIGTMVGTASAGSIRMTECPPDA
PEATFRWVNEGEHLYVEGAGSCVTLPDIYDAVENPPLTYHTVEGDESDTE
TGIWLMTKNIFMESGTTLLLHDDLGCDELRIISSSTNARHLRAFGAYISL
LRTKLTSWSVGNRAPDNGAYDGLNARSFISAVSEMTHPSDTCDGAADDDA
GEARMDIINSEVAYLGYQASESYGISYKVRGYCTDESNPEVFDRVNVRGD
IRFSDIHHNWFGHYSYGHQDGLWEYNTMHNCGQYGFDPHDDSDNLRIHNN
IVYENGNHGIIASKRCNDVSIQNNVVYDNALHGIMLHRSSDQGIIRNNTV
SGSGSACIAIFESFDIEISDNLCTENEEGIRLSMGSSYNKIFDNQVIDTA
GRSLWMYLGSDEVEASAATDGMCTGNLFQNNYLEGSETGVAMTETRDTML
IGNTFIDTDNNEWKDSDGLVWRDNTIDGDFDMRFSDTCVTSESDFDQING
VGSNPSEC*

mRNA from alignment at E-siliculosus-1a_M_contig9:1189738..1201889-

Legend: UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_E-siliculosus-1a_M_contig9.17024.1 ID=mRNA_E-siliculosus-1a_M_contig9.17024.1|Name=mRNA_E-siliculosus-1a_M_contig9.17024.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=mRNA|length=12152bp|location=Sequence derived from alignment at E-siliculosus-1a_M_contig9:1189738..1201889- (Ectocarpus siliculosus Ec864m_EcPH12_78m male)
CATCAAAGCCAACCAACATCCAGACACGTTTACCTTGACACGAACATCAG
CAATCCAAGTGAGCGCGTGGTTGTCCGAAGTCTTACTTACATCCCGGCTT
GGCTGCGTCGCTGTGAGGAGACGAGAGGGGGACAAAACTATGAGATCGTC
AAAGATGGACCGGAGGCGGCGGGCGGCTTCGGGAGGGTTGGGGGGCAGCG
GCGTGCTGCTGTGCTTCGTAGTGATAGGGACGATGGTAGGCACGGCCTCG
GCTGGGAGCATACGGATGACGGAGTGCCCGCCCGACGCGCCAGAAGCTAC
CTTTCGCTGGGTGAACGAGGGAGAGCATCTCTACGTTGAAGGAGCGGGGT
CTTGTGTGACGCTCCCGGATATCTACGACGCGGTGGAAAACCCTCCGCTA
ACGTACCACACGGTTGAGGGGGATGAGAGCGACACGGAGACAGGGTGAGA
GAGGCTCGCTGTGGGCGGGGAGGGATGGGGCTGGAAGGGCTTGTTTCCTT
CGAGGTCTTGGGGTCGCTTATCGGTAGTGAGCTCGCGGCCACTATTGCTG
CTGTGGCAATAACCCTTGTGCTGTCCTCTCTCAACGCTGTGCTGTCCTCT
CACCGTGCCGATTCACCGGTACTTTTTTATGCTGTGCGGCCCACCACACG
TCACACACATGTGAATACAACCAAACGTATCTTACGCCTGTGCCAGAGTT
GTGACATGTTTGTCTCATGGCAGCTTCTTCCGACGGCGAGACACACAAAG
GAGTCCTGGGGCAGAGTCAAGGAGTGGTGATGTTCGCCCTGAGTTGGCGT
GACCAACTTTCGGCCTGGAAATCGATTTTGCTGCTTGCGACAAACACACA
AACAGCCCGCGTCCTACTGTTACATACGTCATATGCGTATGTAGTCTACC
TCCTTTGGCACATTTCAGAGAGCACCGAGCAGCAGAGTTGTGCCGCGGCC
GGAGTCATTCCTCCCGAGTCGCACCTCCCCGTGTCTGTTGTGTCTGCCCC
TTTCCCATCCTGTCTCTGGAGGGTTAGATAGACAAAGGAGACCTACGTAG
CAGTTCGTATTCGATACAAACCAGACCAGAGACCTATAGGGCGCCGAGAA
TCAGATAAAATAATGTCAAAGCTGATGTGCGGGCTCTGATCAAGATGCTG
CCTGCACCGATGGGCTGCGAGGTCCATGGTTTTTCGGGCAGAACCAGACC
GAAACAGCTTGGCAGAAAATAGTTGTACAGGTATGCATACATACTGTAGT
TTGATTTGTTTTTCTTACTGAACAAAACAATACCCCATCCTCCTCCGTTC
TTGCTGTAGTCACATACCACACACGAAGGACACCAAACTGCTTTGTGACT
GCTTTGTGGCACCTGTTGATGTTGCTCCAGGATTTGGTAGAAAAACAGGT
ACTAACGCCAATGCGACAATATGGATGCTTTGCTAATTGATCCTCAACGC
TTCTCTACTTGTATAGGATAGAGATGAAATTTTCTAGAAATCGTCGAGCG
TTGTGTGTACGTCAACCTCAATCTTCAACGATAGACAACAGTGCTGAATG
GAGAGGCCGGGAAGGCGGGAGGTATCGTCCCCTGGTTGTGCGACCAAACG
ATCCAAACAGCATGCCGTGAGAGCAACACAAATTAGGCACCCCGTTTGCC
TTGCGCGAGGATGCCTCCTCGTATTGTTTGGATGGCGGTGCGGTGGATTG
GATGGAAACACTACACCTACCTTACTGACGACAGCATCTGGCTAATGACG
AAGAACATCTTCATGGAGAGCGGGACTACCCTGCTGCTTCACGACGACCT
CGGCTGCGACGTGAGTCCAAGCAAGATCGTACTGGTGAATGGTGTATCCT
GATTTGATGAGCCTGATCCCGGATGTTTGTGGTGATCTCATGTTTCCGCC
CCCCATGGGGTGGAGAGCGGGAACACTCCACTTCTGTTGATTACGGCTTC
AGCAAAGTGTGGATCAAAGCCGAAAGCACGGCTGCTCGCCAAAACAACAA
CCTTGACCTGTCGTTCTCCGGTGACACTCAAGTAGATCGATACTCCGAGC
ATCTTCTGCCTCCGTCAGGCCCCCTGTCCTTCCCCACGTCGCGTCGCCTC
TCGTTGTCCACGTCGTCGTGAGGACTTGTAGCATACCCTCGAACCACCGC
AAGCTGGAATGCCATGGCCAATTTCTCCTTGAGCTATTTTGTCTGTCTTT
TCAGATGAAAACTGTTCATGTGAGAAAAACTGAAGAGCGAACACCTGTGT
GATCCCCATGTATCGTAGTCGCTTCGAACCGCTAAGGCGTGCGATCGTGT
TCTCTCGGTTTGGTTGTTTTTCCTTCTGAACCGTGTTCGTCGTTTGCAGG
AACTGCGGATAATCTCGAGCTCGACAAACGCACGCCATTTGCGAGCATTC
GGAGCGTACATCTCCCTTCTCAGGACAAAGGTTAGCGCGTGGTATCTAGG
TCACGAAAACGGTTATTGCAGCAGATGTCGACCCGTAGAACACCATGTCT
TGTGCGGGGTTCGCGTGAGAGGAGTGACAATCTGTGCATCGATTGACGGA
GGCATTTCCTTCCGTGGCTATCGCCACACGTCGAACGAGCCACTGTGTCC
ATGTTGCCCGGAGCTCATGTTCGCGGGTCGTTTCCTGGATGCGTCCGAGC
TGAGCTACATAGGTCTTTGCGTATTTTTGTCGACGTTGTTTGTTTGACCT
GTCTTGGGGGTGATTGTGTTGGGCTTTGGTCTTAACCCCCACGTCACGGA
TCATGCCGCGTTGCTATTTGTATCAAGTCAACAGGTAATATTTGTTTGGA
TCCTCGTCGTGTGTATGTAGCTGCAGCTGTGTGTCATGAGCTGTGAAACG
TGTGTGCTTAGGCCGGTCTCTTCTTGGTTCCTGATGCTGTTGCCGTAGCT
GACGTCTGAAGCTGCAGCTGCGTATCATGAGTTGTGAAAGCTTGTGCTGA
CCCTCGTCCCATTTAACGGTATTCATGTTCCTGTGTTTGCTGCCTGCGTC
TGTAGCTGCAGCAGCACAGGAGAGTAGGACTTGCGAAGATGTGTGACGAG
CCCTCGTCTCTTTTTGATGCTTCTGCCGTTTTTTGCCGTAGCTGACCTCT
TGGAGCGTCGGGAACAGAGCCCCCGACAACGGCGCGTACGATGGACTCAA
TGCGCGGTCCTTCATTTCGGCAGTGAGCGAGATGACTCACCCCTCGGACA
CGTGCGATGGCGCAGCCGATGACGATGCTGGGGAAGCGAGAATGGACAGT
GAGTTGTTGGTTTTGTTTGTTGGTGTTGGTGCCTGCGTTGCTTTGTTGTT
GTTGGTGGTGGTGTTGGTGCGGCTATGGTTGTTGTTGTTGTTGGCGTCGT
TGTTGCTGTGCTGTTGTCGTGAGGTTGATGTTGTAGTTGGTGTTGGTATT
GGTGTTGTAGTTGTTGGTGATGTCGGTGTTGGTGCTGATGTTGGTGTTGG
TATCGATGTTGGTGCTTGTGCTAACGTTGGTGATGGTGTTGGTGCTTGCG
TTGGTGTTGTTGCTGCTTTCGTAGTTGTTGTCGTTGGACCAGATCGGACC
GGAACGATGGCAAAGAATTGCGTTGGTCGCAAGAAGGTGTGGTCTGTCTC
AATTGCGGTCGGTGGATCAAGCTGCCACGCTCCCAAGACCGTCCCACCTG
TGCCTCACCTACGCCGGTGCTCTGCCACCAACATCAGTAGTGGTCGTTCT
TGCAGACATGGATTAAATCAAGTTTTCGACGTCATGACCTCCTATGAAAT
CTTACCGTGTCCCAAATCTGTCTGTCTGTCTGTCTGCCTCTCGCTCTCTC
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTCCTCCTCCTCCT
CCTCCCCCCCCTGTCCGCTCTTCCCCGATTATCTCTCGTTGCCACGCGTG
TGTACAGTCATCAACAGCGAGGTGGCCTACCTAGGCTACCAGGCATCGGA
ATCGTATGGCATCAGCTACAAGGTGTGTTTCGAACGCTCGCGGAGGTTCG
CTGAGATATGGGTTGCTGCTCTGCTGTTTTTTTCTCTGTCTGTCGGTGCG
TTCGGTTGGTCGATGGTTGGCTTCGGGAAAGACCGAGGAGCAAATGATAG
CGGTAGCCATCAAGAGCAAGCATCAACAGCAGAACAATGGCATCATCCCT
CAAAAGCAAGCAACAACAGCAGTTTCCATCAAGACCAAGCGACAAGAGAA
CCGTCAATAGAGCACGCGTAACGACGAGGCGAGCAACCGACGACAACAAC
TACCATGAGCACACTACACAGTGTTAAGAAAGTACAAACACTGAACGCTG
CCTCTTGATGCCCACAGGTGCGAGGGTACTGCACGGACGAGTCCAACCCG
GAGGTGTTCGACCGCGTTAACGTTAGGGGAGACATCCGCTTCTCGGACAT
ACACCACAACTGGTTCGGACAGTGAGTACTTTATGTGTTGGACAGTTCAG
TATTTTGACACATGGTACGACATGTGTAACCCTGTTCTATTGGTGGCCTT
GGTGCCGATTCAGGTAGACAGTGCTCTGCCGTTTAGGGCTGCGTCGAACC
TGCTATTTTTTTTCTTGACGCGCACATGAATATACCGTGCTGACGCATGC
CCGTGCACGTCAATCTCCCACACTACTGACAGTCTTGCTAGACCGCCCAT
GGGACACCCATGGGTCACCCATGGGACACCCGTGGGTCACCCATGGCCGC
CCATGGGCACTTCATGGGTGGCCCATGGGCAGCCCATGAAGTGCCCACCA
TGGGCACTTCAACCTGGAACTTCGCCACCGGCATATCAACCTAGAACTTA
TCCACCTTCACAACAACCTAGAACTTAGCCACCAGCGCCAACCTAGCACG
TATCGTGTAGGCTTGGGGTTAGGGTTATGATTAGCGTTAGGTTATTAGCC
GATATCGTGCCCGTTCTAAGCAGAAAATCCATTGTTACGGAGTACACTGG
TTCGGTTGAAGGGCTCTCATTGGCCGCCCATGGGCAGCCCATGTGGGCGG
CCCATGGGAATGCCCATGGGTAGCCCATGTAGGCGGCCCATGGGAATGCC
CCATGGGTGACCTTTGGGAAAACTGTTGAAAGTGTGGGAGGCCCGTACAC
GTGCGGTATGAAAATCGTGAGGTGGGAGTTACTTGTTGAGCGCATTGACG
TGGTTGTGTCCTCCTTCTTTTTTTGTCTACTTCGCTTGGCTCTTGTTGGT
TTATTTTTTCGATTTTTTACGTTTCACCCGCCAAGTCCTTCGTATCGCGT
TCGGGGAGCTTGAGCCGTATGCTCTTGAAAGCCGAAACTTGTAAACAAGA
AAACGAAAATACGTTGTCAGCAAGAGGGAGAAAACAAATCGGTATCAGGG
AAACAAAAAGCAAATAGTTAACAAGAAACCAAATAACAAATCGCGCCAAA
CACAACCCTTTTAGACACTTCTGTATTGATATGCGACCGCCGTCGCTCGG
TGGGGTGCCGATCTTGCCCGCTGGCCCTGTTTCTTTTTGGTATATTCGGC
GAAGGTTGCGGTTGTTCGCCTTACACCCGCGTGTCATTCCTCCACGGGCC
CCGAGTGGAAACCATGGAACACCCGCCACCTAACCCTCCCCCCCTCCCGA
TCACCGTTGACACCGGCGGCCATGGTTTCCACCGCTGTGAGGCCCCCTTA
CATAACTCGTTCCGTGGACCCACTCCCCCGGATGGATTCACCCTCGACAT
CCTCGAAACCCTTTCTTTTTGTTTCTTTTTTTTTTCTGTTGCAGCTACTC
GTATGGACATCAGGATGGGCTGTGGGAGTACAACACCATGCACAACTGCG
GGTGAGCTAGCTGCTAGATAGCCAAGACGGCGGTTTTAGGGGGTTAGCGG
AAGCTGCAGAAGCCGATGCGTTGGGGGGGGGGGGTTCGGTGCGTCATGTT
TTGGGTTTAAGTCTGGATTGGAAGTGCAAGCACACGAGAAAAACAGAAGG
CTTTCGTGGCTATTTCTTGACAAATGCCCCCAAGCGGCAGATTTCGGCAT
TTCAACCATCGTTTCTTCTCCGGGAAGCGAGAGCGTACGAGGAGGAGGAG
GGGGTACTCGACAGAGGTATACACGTTTGTGGGTGTGGAACCAAACAGCC
GAGCAACCTTTTAATCTTTTTAACAAACATAAGGACAGATGATGCGAAAT
GCTCTTGTACGTGTACCAGGTTTGGTCGTGAGGTAAGTCGGATTCGTGAA
TATTTGCGGATCATTCACCGCGGTCGTTCCCCATTTCACTGGTAAACGTT
GCCAATGTTTGCCCTCTCCGTCGTACGTCTCTGTGCACGTGAGTCCCCCC
CCCCTCCCCTCTCCCCATCCGTGCTGCCCCCATGTATTGGTTTTATCTGT
CCTGGACCTTACAACTTTCATTCTTTCTGTTCGAATGGCCGTTTTTGCCC
TCCCACAGGCAATACGGGTTCGACCCTCACGACGATTCTGACAACTTACG
GTGAGCTTCGATTGGTTCGCTGTGCGTGCGTTCTTACCACTGGTGTCTCC
GTGCAATGACAATGCGACACCTATAAGCGATGATTCAAGTGGGATCGATT
GATCGATTGTGTGAGGCTTGGCTCATTTCCTCGTGTTTAGTTGTGTGTGT
GGATGCGTTCGTGTATCATTTACGCAACTATTATGTGCTAGCATGTAGTA
ATATCCATGCAGACGTGTTTATTATACCATGTTTATGCTTTTCTTTCTTT
TCTTCAGGCCGCAGTTTTCTTCGGGCTCGTCTCCCCCGCTGTTTTTGCCA
CGCTCCCCTGCTCACCACCGGTGCCAAGTGGGGATTTTCGTTCACTTTTT
GTTGTGTTGCGTTTGTCGTGGTTTGTGCCTTCGCCGTTCCTAGGTGTGTG
TCCGTTGCTCTCCCTCTCTCTCTCAGTCGTCTTCTCCCCACCCCTGCAAC
CTATATTCGCCCACGCTCTTTCGCATTCTTGGCTGCCGACACATCCCGAA
TCGTCCTCAACTTCATGTTGCGATGATGAAGGATCCACAACAACATCGTG
TACGAGAACGGTAAGTAGAATTCGACGTCGCGCATTTCTTTGTCCGAGGA
CTTTAAGGAGAGCCACGAAGGAGCTTGAGTAGTAGATCGTATCCTGATGT
TGTGTGTGTTTTTGCGTGTATAAGTGACAACGTGACCAGCGTTGAAGGTG
GGAATTCCCATGGGGGCGAGGAATGCAAACCAACGTGAGAACGTACGCAG
CAGCATAGACATTTCATTCTCTTCGTGTGTGACCCCTGGTTCAGAACAAC
TACACTACTACTGTAGGTAAAACTAGAAGCCGTCGGTGGTAGGTAGATCG
TTACGTCCGGTGCTTACGGCGGCGTGCTTGTGTTTTCACTCTATGAGCGG
TTTTCGGCTGCGATTGGCGAAGGCTGATAACGAAAAGCCTGTCTGTTTGA
TGTAGAGAGGTTTTTTCGTCTTTTGTCGTTATCTTCGGAGTGGAAATTGC
GACCACCCACGGATTTCTCTCTCTTTTGGTTTTTGTTTGGGGGGTTGGCC
TTGGTAGAAAACGTAAAAAAAGCGGTCGATGGCCGTAAGAATCGGTCGAA
CTACCGCAACTGACGATGCTGACTCGGCTCTGTCCTCTCTATTGTTTTGC
TCCTGCAGGCAACCACGGTATCATCGCGTCGAAGAGGTGCAACGACGTGT
CCATCCAGAACAACGTGGTGTACGACAACGCCCTCCACGGCATCATGCTC
CACCGCTCCTCCGACCAGGGAATCATAAGGAACAACACCGTCTCTGGCTC
CGGGAGTGCTTGCATTGCTATCTTCGAGTCCTTCGGTAGGCTACCGCTGT
GTGATCGCGCTGTTTGGTGATCTTCCCATTGCACGTAAACCTCTAGATTA
CACATCACGTCGATTCATGGTTTGTGGTTCGCTCCTATTTTGCGCTTTTC
TTGTGCTACAGTACAATGCCGTACGTACGATGGGCGGCTGGCGTAAGCGA
GAGCACCTCCACCGACAATTCAGGGCCCGCACCCGCCCCTAGTTTTAGTC
CCCCCTTTCTCAACTGTTTCATACCGCACAAGAGTACCAGTTTATAGTAA
TGGCGCAAACACGGTCCCTCCCTTGTCCCGTTTTGCGTTGTGGTAGACAT
CGAGATCAGCGACAACCTCTGCACGGAGAACGAGGAGGGCATCCGGTTGT
CCATGGGGTCGTCCTACAACAAGATCTTCGACAACCAAGTCATCGACACC
GCTGGCCGTGAGTTCTGCTATCATATCACGTCTCAGTTTTCATGGTAGCG
CCTATTTTGTCGGCTGTCGTCAGCGTGGCGCAGTTGCCTCCCGGCCTTCG
GTTTTCTGTGCTTGCCCATGCCCCCTTTGTTGTTCTGTTAGGTTTTGGGA
TAACCCTAAACCTACCTAATCCTAACCCTAAACCCTAACCTGTGTCTTGT
CGTTGGTTTGGTGTGTTGCTCGTACCGGTGGGACAAGGGACGGGGCAAAT
CTCCCTTCGAGTGGTTCCTCTCTTGTTGTTGGTTGCTGTTGATAATGTTG
GTGTTGTCTTCGCATGAAAAACCGTTCCGATCTCTCTACCCAGTACCTTC
TGTCTATGCTACCGTAGTCTGACACTTGACGCAGTCGATTCCCATGGCCA
CTGGCTATCTTGCCCGAAGAGGATCCGAGGGAACGGAATTCAATAGAAAT
CAACGACAGATAGCCTGGAACCCTCCGCTTTGGCTAGGTTCATTGCTCCT
GATAGTAAGACAAGGAACGCGACCAATCTTCGATTCGTATATGGCCTGTC
GTTCACAAGAGGGAGCCATTTTGAAGTTTTTGGTGTAGCGAGCGCCTCCG
TTCATATGAGATGGAGTCCGTCATGACAAGTTGAAAGGATGTGGGTCACT
CACTCTCTTCCGCGGGATAAATACCACATTCCAGTTCGCGGAATCGGCTG
CGTTCCGCTTCGGCAGAACGTGAGGGTTGTGCCAGATGTGCCGCCTTGTG
CCACATGCCATTTTCAATGCGTTCATTGGCACGTCTTCCTGTGTACGGTT
TAATGGATGACTTTCATTCCACAAGCTGGAAGGCATTATTGGTAGATTTT
CCTTGCACGGTGTACCGTACGAATGCTCGTAGTAGTTCGATTTTTCCTTT
CTGTTCATTTTTCACACAACTCCGCGGAATTCATCCCGTTTCTGCCTCGC
AGGATGTGTTTTGTTCCGTTCGCGAGGTTTGTGATTGTTTCCCTTCCATT
CGGCGTATTTCTCTACACAGAGAGGAATGTGTTGGCTTGGTTTAGCGTTA
TATGTTGATGTGGAGTCCGTGTGCTCTATGAATCTTCACTCTGTCACAAA
GGGGTACCTCCTGTGCCTTTCATTCCTTGGTGCCGTGAGTGAATCAGTCA
TCGATACATTCTTGCTGACAGGTCACTTCTTTTTTAATTCTTATGGCGGC
TGGTGTCAAGGCCTGCGTGTTCGTTTTGTCTACGCGTGAATGCTCGTGTG
TGCCGATTGGTTGCAGAGTCCCCGGTGGTTGTTCCTGTTGCAAAATCACA
AGCTCGGGCTATCTATCGCCACATGCAACCCTTTCCCCGACTTGGGGGAT
TATTTCGATTGCACTTCAAGATCCATCTGGATGTAGCTGAGATCCAACGA
CATCGATGGTCCCCAGCCTTCACAGACTCATCCACAGACTTCCACTTTTC
GGTTACGCTTCTGGGGCCATCTGGATGTACCCGAGATCCGACGCGACCGA
TAGGCTTCAACCTTCACAGATTCATGCCCCCGGTAGTTCATTTTTCCGGG
TACGCTACGAGGGCCATCTGGATGTACCGGGGGTCAGACGAGATCGAGGC
CCCCGTCGTGACTGACTCAACCCCCCCCCCGACTTGCATTTTTCGGTTAC
CCTACAGGGGCCATCTGGATGTAGCTGCAATCAGACGAGACCGATAGACC
TCAGCCTTTCACTCAACCCCCCGCCCCGACATGCACATTTTCGGTTACCT
CAAGGGACCACCTCGATGAACCTGAGTTCCGACGAGAGCGAGAGGCCTCA
GCCATTCACTCAACCCCCCCCCCCCCGATATACATTTTTCGGTTACCCTA
CAGGGTCCCTCTGGATGTACCTGGGGTCCGACGAGGTCGAGGCCTCCGCC
GCTACTGACGGCATGTGCACGGGCAACCTATTCCAGAACAACTACCTGGA
GGGGTCCGAGACCGGGGTGGCCATGACCGAGACTCGGGACACGATGCTGA
TCGGGAACACGTTCATCGACACCGACAACAACGAGTGGAAGGACTCTGAT
GGGCTCGTGTGGCGGGTGAGGGAGGCAGGAGACTTGCTAGTATAGTGTGT
TGCAGCCGTAACTGATTGCGCGCTCGAGAGGACGGAGCGACGTTCCCAAA
AGACCGATGGCCAGGGCGGGGGGGAAGGGGTTATTCTCGTGCGTGTTTTC
ATGTTTTTCAGCATGAACCAGGGTCGGGCTGAAGGTGGGGGTCGGAAAGA
GGGGGATTGGAATATTTTGTCTGCGGAAACCGCCAACAGGAGGACGGATG
TGCAAACACATGGGAGAATTATGGCTTGGTTGTTGCTGAGCATAAGAATA
GTTTCGCCTCGAATGCAATGGTTAGGTTTTTTGGTGTGGTGTAGCGAGTT
CTTGAGCGTGTTGAGGTAGTCTCTGGTTGTCGAAAGCTTCTGGTTGTTGA
ATGGTTCAGACGGGTTTCGGTGCGACTGGCGCGGTGGTATCGTTTGAAAA
TTGCTTCGGTTTTGTTTTTGGCGTCTCCATGTTTCCCCCTCCTTTACTTC
CCGCGTCACCTATCCATGCCTGGCGGATCGTGTTCGCAACTTGAGAGTAT
GGACAAGCCGCGATTGTGCTCAATCCGCCATAGTTGCCCCATTTGTTTTC
TTCGCGATCACATCCCGGCCGGCATGATTGGAAACCTGGTGCGCCCCCGC
TCATCCGCTTTTTATTGTTTTTAGCTCGTCCACCTCCCACGGAGCCATTC
TATCCGTACCTCTCGTGTACGTTTCGATCTCGTCTTCCACCAAGCGACCC
TACCTCTGGGTACATTTTGATTTCGTGCTCCACCAGCGGCCCAAACCTCA
TCTCTTCCATCGCACACGGAGCGACCCTCTTCCCATCTCGTTGCACGTTT
CGATTTAGTTTTCTGCTGCAACGTTGTATTGGAGAACCAACTGGACGCTG
ACTTTGGATGCGTGCCCCCTCTATCCGCTCGCCCCCACAGGACAACACTA
TCGACGGAGACTTCGACATGAGATTCTCCGATACCTGTGTAACGTCCGAG
AGCGATTTCGACCAGATTAACGGCGTCGGCAGCAATCCGAGCGAGTGCTA
ATCGTTGGTCGGTCGAATCACTAGCTTGTGTGTCACATACCACATAATGA
TGAGCGGGAGCCACCTCGGCATATTTTTGGAATAGTGTGTTGCTTCGACG
ATCGCTGACATTGGCTGTAAAATAACAACGTGACACGATCGATTTCAGGG
AGTATCGCAGGGTATCTGTGGCGTGGAGATTTGAATTCATAACGTACGCG
GGAGGCCGCATTCCTGCAACTAACTGTACACTTAGTTGCCTGCGGGCGAC
CTACAAGTAGTGTGAAGCCACTCCTTCTTCCTTAGTTTCGGTGAAGTACG
GAATCGCTTAGGCATCGGGGGGGGGGAGTGTTCCCGCCCTGAGGCAGACG
GTGCGATACGTTCCGGTTGAACCCTTGATTTTCGATCGAGACTTGTCTGC
GGCCTACTAGTACAAGGGTGATTTCTTGTTGATACTCAACGGCTCAACTG
CTTTCGCTGAGAAGGGCGGGAGCATAGATACAGCTTCTTGCGGGTACAGG
TTATCGATGCAAACGTCTGTGTGAAAATCCTCTTGCCCTCTAGCCTAGAT
CGTTCAAGCAGCGAGGTGCGGGTTATGCGAGTCTTTCTCTCGGTTCCCGC
GAATGTCATTTTCTACCAGTCCGTGCGCCGCCGGCGTCGTCGTCGTCTTG
TTTACACGTCTCCTTTTGCTCTTGCGGTCAAGGGGGTGTCGTCGACCTTT
GCTTTATCCTAGGGCCGAGGGGAGGTTTACGGTAACGGCATCAGCCGTGT
TCACGACAGATGCTTCGTCTTGGTTGTTCTTGGTTGTTCTTGGTTGTCGA
GAGGGATTCGGTACATTCGAAAAAAGCCCCCGGCTCCTGCATTTGTGTGT
TG

Coding sequence (CDS) from alignment at E-siliculosus-1a_M_contig9:1189738..1201889-

>mRNA_E-siliculosus-1a_M_contig9.17024.1 ID=mRNA_E-siliculosus-1a_M_contig9.17024.1|Name=mRNA_E-siliculosus-1a_M_contig9.17024.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=CDS|length=3054bp|location=Sequence derived from alignment at E-siliculosus-1a_M_contig9:1189738..1201889- (Ectocarpus siliculosus Ec864m_EcPH12_78m male)
ATGAGATCGTCAAAGATGGACCGGAGGCGGCGGGCGGCTTCGGGAGGGTT
GGGGGGCAGCGGCGTGCTGCTGTGCTTCGTAGTGATAGGGACGATGGTAG
GCACGGCCTCGGCTGGGAGCATACGGATGACGGAGTGCCCGCCCGACGCG
CCAGAAGCTACCTTTCGCTGGGTGAACGAGGGAGAGCATCTCTACGTTGA
AGGAGCGGGGTCTTGTGTGACGCTCCCGGATATCTACGACGCGGTGGAAA
ACCCTCCGCTAACGTACCACACGGTTGAGGGGGATGAGAGCGACACGGAG
ACAGGATGAGATCGTCAAAGATGGACCGGAGGCGGCGGGCGGCTTCGGGA
GGGTTGGGGGGCAGCGGCGTGCTGCTGTGCTTCGTAGTGATAGGGACGAT
GGTAGGCACGGCCTCGGCTGGGAGCATACGGATGACGGAGTGCCCGCCCG
ACGCGCCAGAAGCTACCTTTCGCTGGGTGAACGAGGGAGAGCATCTCTAC
GTTGAAGGAGCGGGGTCTTGTGTGACGCTCCCGGATATCTACGACGCGGT
GGAAAACCCTCCGCTAACGTACCACACGGTTGAGGGGGATGAGAGCGACA
CGGAGACAGGCATCTGGCTAATGACGAAGAACATCTTCATGGAGAGCGGG
ACTACCCTGCTGCTTCACGACGACCTCGGCTGCGACCATCTGGCTAATGA
CGAAGAACATCTTCATGGAGAGCGGGACTACCCTGCTGCTTCACGACGAC
CTCGGCTGCGACGAACTGCGGATAATCTCGAGCTCGACAAACGCACGCCA
TTTGCGAGCATTCGGAGCGTACATCTCCCTTCTCAGGACAAAGGAACTGC
GGATAATCTCGAGCTCGACAAACGCACGCCATTTGCGAGCATTCGGAGCG
TACATCTCCCTTCTCAGGACAAAGCTGACCTCTTGGAGCGTCGGGAACAG
AGCCCCCGACAACGGCGCGTACGATGGACTCAATGCGCGGTCCTTCATTT
CGGCAGTGAGCGAGATGACTCACCCCTCGGACACGTGCGATGGCGCAGCC
GATGACGATGCTGGGGAAGCGAGAATGGACACTGACCTCTTGGAGCGTCG
GGAACAGAGCCCCCGACAACGGCGCGTACGATGGACTCAATGCGCGGTCC
TTCATTTCGGCAGTGAGCGAGATGACTCACCCCTCGGACACGTGCGATGG
CGCAGCCGATGACGATGCTGGGGAAGCGAGAATGGACATCATCAACAGCG
AGGTGGCCTACCTAGGCTACCAGGCATCGGAATCGTATGGCATCAGCTAC
AAGTCATCAACAGCGAGGTGGCCTACCTAGGCTACCAGGCATCGGAATCG
TATGGCATCAGCTACAAGGTGCGAGGGTACTGCACGGACGAGTCCAACCC
GGAGGTGTTCGACCGCGTTAACGTTAGGGGAGACATCCGCTTCTCGGACA
TACACCACAACTGGTTCGGACAGTGCGAGGGTACTGCACGGACGAGTCCA
ACCCGGAGGTGTTCGACCGCGTTAACGTTAGGGGAGACATCCGCTTCTCG
GACATACACCACAACTGGTTCGGACACTACTCGTATGGACATCAGGATGG
GCTGTGGGAGTACAACACCATGCACAACTGCGGCTACTCGTATGGACATC
AGGATGGGCTGTGGGAGTACAACACCATGCACAACTGCGGGCAATACGGG
TTCGACCCTCACGACGATTCTGACAACTTACGGCAATACGGGTTCGACCC
TCACGACGATTCTGACAACTTACGGATCCACAACAACATCGTGTACGAGA
ACGGATCCACAACAACATCGTGTACGAGAACGGCAACCACGGTATCATCG
CGTCGAAGAGGTGCAACGACGTGTCCATCCAGAACAACGTGGTGTACGAC
AACGCCCTCCACGGCATCATGCTCCACCGCTCCTCCGACCAGGGAATCAT
AAGGAACAACACCGTCTCTGGCTCCGGGAGTGCTTGCATTGCTATCTTCG
AGTCCTTCGGCAACCACGGTATCATCGCGTCGAAGAGGTGCAACGACGTG
TCCATCCAGAACAACGTGGTGTACGACAACGCCCTCCACGGCATCATGCT
CCACCGCTCCTCCGACCAGGGAATCATAAGGAACAACACCGTCTCTGGCT
CCGGGAGTGCTTGCATTGCTATCTTCGAGTCCTTCGACATCGAGATCAGC
GACAACCTCTGCACGGAGAACGAGGAGGGCATCCGGTTGTCCATGGGGTC
GTCCTACAACAAGATCTTCGACAACCAAGTCATCGACACCGCTGGCCACA
TCGAGATCAGCGACAACCTCTGCACGGAGAACGAGGAGGGCATCCGGTTG
TCCATGGGGTCGTCCTACAACAAGATCTTCGACAACCAAGTCATCGACAC
CGCTGGCCGGTCCCTCTGGATGTACCTGGGGTCCGACGAGGTCGAGGCCT
CCGCCGCTACTGACGGCATGTGCACGGGCAACCTATTCCAGAACAACTAC
CTGGAGGGGTCCGAGACCGGGGTGGCCATGACCGAGACTCGGGACACGAT
GCTGATCGGGAACACGTTCATCGACACCGACAACAACGAGTGGAAGGACT
CTGATGGGCTCGTGTGGCGGGGTCCCTCTGGATGTACCTGGGGTCCGACG
AGGTCGAGGCCTCCGCCGCTACTGACGGCATGTGCACGGGCAACCTATTC
CAGAACAACTACCTGGAGGGGTCCGAGACCGGGGTGGCCATGACCGAGAC
TCGGGACACGATGCTGATCGGGAACACGTTCATCGACACCGACAACAACG
AGTGGAAGGACTCTGATGGGCTCGTGTGGCGGGACAACACTATCGACGGA
GACTTCGACATGAGATTCTCCGATACCTGTGTAACGTCCGAGAGCGATTT
CGACCAGATTAACGGCGTCGGCAGCAATCCGAGCGAGTGCTAAGACAACA
CTATCGACGGAGACTTCGACATGAGATTCTCCGATACCTGTGTAACGTCC
GAGAGCGATTTCGACCAGATTAACGGCGTCGGCAGCAATCCGAGCGAGTG
CTAA