mRNA_E-siliculosus-1a_F_contig10951.659.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig10951.659.1
Unique NamemRNA_E-siliculosus-1a_F_contig10951.659.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig10951.659.1 vs. uniprot
Match: A0A6H5KZU9_9PHAE (Protein O-GlcNAc transferase n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KZU9_9PHAE)

HSP 1 Score: 254 bits (650), Expect = 1.920e-76
Identity = 122/124 (98.39%), Postives = 123/124 (99.19%), Query Frame = 1 
Query:   13 QQSARYVLDRTLLPTRATYGVPEDRFVLCNFNQLYKMDPAIFSTWMSVLKRVSNAVLWLLRFPPAGEANIRMEARKRGVREEQLHFTDVATKEEHIKRGYLADLFLDTPSCNAHTTGCDILWSG 384
            +QSARYVLDRTLLPTRATYGVPEDRFVLCNFNQLYKMDPAIFSTWMSVLKRV NAVLWLLRFPPAGEANIRMEARKRGVREEQLHFTDVATKEEHIKRGYLADLFLDTPSCNAHTTGCDILWSG
Sbjct:  768 KQSARYVLDRTLLPTRATYGVPEDRFVLCNFNQLYKMDPAIFSTWMSVLKRVPNAVLWLLRFPPAGEANIRMEARKRGVREEQLHFTDVATKEEHIKRGYLADLFLDTPSCNAHTTGCDILWSG 891
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BLAST of mRNA_E-siliculosus-1a_F_contig10951.659.1 vs. uniprot
Match: A0A5D6YC07_9STRA (Uncharacterized protein (Fragment) n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6YC07_9STRA)

HSP 1 Score: 201 bits (510), Expect = 2.600e-60
Identity = 92/124 (74.19%), Postives = 104/124 (83.87%), Query Frame = 1 
Query:   13 QQSARYVLDRTLLPTRATYGVPEDRFVLCNFNQLYKMDPAIFSTWMSVLKRVSNAVLWLLRFPPAGEANIRMEARKRGVREEQLHFTDVATKEEHIKRGYLADLFLDTPSCNAHTTGCDILWSG 384
            +QSAR VLD    PTRA YGVPED FV CNFNQ+YK+DPA F+TWM++LKRV +A+LWLLRFPP  EANIR EA  RGV+  +LHFTDVA K+EH+KRGYLADLFLDTP CNAHTTGCDILW G
Sbjct:  234 RQSARDVLDAAKCPTRAHYGVPEDAFVFCNFNQVYKIDPATFATWMNILKRVPSAILWLLRFPPLAEANIRAEAAARGVKAHRLHFTDVAPKDEHLKRGYLADLFLDTPECNAHTTGCDILWGG 357
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BLAST of mRNA_E-siliculosus-1a_F_contig10951.659.1 vs. uniprot
Match: A0A7S2BCC6_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2BCC6_9STRA)

HSP 1 Score: 204 bits (518), Expect = 2.850e-60
Identity = 96/126 (76.19%), Postives = 109/126 (86.51%), Query Frame = 1 
Query:   13 QQSARYVLDRT--LLPTRATYGVPEDRFVLCNFNQLYKMDPAIFSTWMSVLKRVSNAVLWLLRFPPAGEANIRMEARKRGVREEQLHFTDVATKEEHIKRGYLADLFLDTPSCNAHTTGCDILWSG 384
            +QS+RY+LD T     TRA YGVPED+FV CNFNQLYK+DPAIF  W SVLKRV N++LWLLRFPPAGEANIR EARKRGV+EEQ+ F+DVA KEEHI+RG LA++FLDTP CNAHTTGCDILWSG
Sbjct:  350 RQSSRYILDETDSEQKTRADYGVPEDKFVYCNFNQLYKIDPAIFDVWCSVLKRVENSILWLLRFPPAGEANIRAEARKRGVKEEQIFFSDVAPKEEHIRRGKLAEVFLDTPQCNAHTTGCDILWSG 475
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BLAST of mRNA_E-siliculosus-1a_F_contig10951.659.1 vs. uniprot
Match: K3X4T5_GLOUD (Uncharacterized protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3X4T5_GLOUD)

HSP 1 Score: 201 bits (510), Expect = 4.650e-60
Identity = 90/124 (72.58%), Postives = 105/124 (84.68%), Query Frame = 1 
Query:   13 QQSARYVLDRTLLPTRATYGVPEDRFVLCNFNQLYKMDPAIFSTWMSVLKRVSNAVLWLLRFPPAGEANIRMEARKRGVREEQLHFTDVATKEEHIKRGYLADLFLDTPSCNAHTTGCDILWSG 384
            +QSAR VLD    PTRA YGVPED+FV CNFNQ+YK+DP  F+TWM++LKRV N++LWLLRFPP  EANIR EA+ RG++  +LHFTDVA K+EH+KRGYLADLFLDTP CNAHTTGCDILW G
Sbjct:  245 KQSARDVLDVEKCPTRAHYGVPEDKFVFCNFNQVYKIDPQTFTTWMNILKRVPNSILWLLRFPPIAEANIRAEAKARGIKSHRLHFTDVAPKDEHLKRGYLADLFLDTPECNAHTTGCDILWGG 368
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BLAST of mRNA_E-siliculosus-1a_F_contig10951.659.1 vs. uniprot
Match: A0A2P4Y2W6_9STRA (UDP-N-acetylglucosamine-peptide N-acetylglucosaminyltransferase (Fragment) n=1 Tax=Phytophthora palmivora var. palmivora TaxID=611791 RepID=A0A2P4Y2W6_9STRA)

HSP 1 Score: 196 bits (497), Expect = 4.340e-59
Identity = 91/125 (72.80%), Postives = 106/125 (84.80%), Query Frame = 1 
Query:   13 QQSARYVLDRTLLPTRATYGVPEDRFVLCNFNQLYKMDPAIFSTWMSVLKRVSNAVLWLLRFPPAGEANIRMEARKRGVREE-QLHFTDVATKEEHIKRGYLADLFLDTPSCNAHTTGCDILWSG 384
            +QSA+ VLD    PTRA YGVPED+FV CNFNQ+YK+DP  F+TWM++LKRV N+VLWLLRFPP  EANIR EAR RGV+++ +L FTDVA K+EH+KRGYLADLFLDTP CNAHTTGCDILW G
Sbjct:  135 KQSAQEVLDTEKCPTRADYGVPEDKFVFCNFNQVYKIDPVTFTTWMNILKRVPNSVLWLLRFPPIAEANIRAEARARGVKDQTRLIFTDVAPKDEHLKRGYLADLFLDTPECNAHTTGCDILWGG 259
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BLAST of mRNA_E-siliculosus-1a_F_contig10951.659.1 vs. uniprot
Match: A0A8K1FGH6_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FGH6_PYTOL)

HSP 1 Score: 201 bits (510), Expect = 1.880e-57
Identity = 91/124 (73.39%), Postives = 106/124 (85.48%), Query Frame = 1 
Query:   13 QQSARYVLDRTLLPTRATYGVPEDRFVLCNFNQLYKMDPAIFSTWMSVLKRVSNAVLWLLRFPPAGEANIRMEARKRGVREEQLHFTDVATKEEHIKRGYLADLFLDTPSCNAHTTGCDILWSG 384
            +QSAR VLD    P+RA YGVPED+FV CNFNQ+YK+DPA F TWM+VLKRV N++LWLLRFPP  EAN+R EAR +GV++ +LHFTDVA K+EH+KRGYLADLFLDTP CNAHTTGCDILW G
Sbjct:  725 KQSARDVLDVEKCPSRAHYGVPEDKFVFCNFNQVYKIDPATFRTWMNVLKRVPNSILWLLRFPPLAEANLRAEARAQGVKDNRLHFTDVAPKDEHLKRGYLADLFLDTPECNAHTTGCDILWGG 848
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BLAST of mRNA_E-siliculosus-1a_F_contig10951.659.1 vs. uniprot
Match: D0NXA5_PHYIT (Protein O-GlcNAc transferase n=2 Tax=Phytophthora TaxID=4783 RepID=D0NXA5_PHYIT)

HSP 1 Score: 197 bits (500), Expect = 4.710e-56
Identity = 92/125 (73.60%), Postives = 106/125 (84.80%), Query Frame = 1 
Query:   13 QQSARYVLDRTLLPTRATYGVPEDRFVLCNFNQLYKMDPAIFSTWMSVLKRVSNAVLWLLRFPPAGEANIRMEARKRGVREE-QLHFTDVATKEEHIKRGYLADLFLDTPSCNAHTTGCDILWSG 384
            +QSAR VLD    PTRA YGVPED+FV CNFNQ+YK+DP  F+TWM++LKRV N+VLWLLRFPP  EANIR EAR RGV+++ +L FTDVA K+EH+KRGYLADLFLDTP CNAHTTGCDILW G
Sbjct:  722 KQSARDVLDTEKCPTRADYGVPEDKFVFCNFNQVYKIDPVTFTTWMNILKRVPNSVLWLLRFPPIAEANIRAEARARGVKDQTRLIFTDVAPKDEHLKRGYLADLFLDTPECNAHTTGCDILWGG 846
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BLAST of mRNA_E-siliculosus-1a_F_contig10951.659.1 vs. uniprot
Match: A0A0W8DU52_PHYNI (Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY n=20 Tax=Phytophthora TaxID=4783 RepID=A0A0W8DU52_PHYNI)

HSP 1 Score: 197 bits (500), Expect = 5.150e-56
Identity = 92/125 (73.60%), Postives = 106/125 (84.80%), Query Frame = 1 
Query:   13 QQSARYVLDRTLLPTRATYGVPEDRFVLCNFNQLYKMDPAIFSTWMSVLKRVSNAVLWLLRFPPAGEANIRMEARKRGVREE-QLHFTDVATKEEHIKRGYLADLFLDTPSCNAHTTGCDILWSG 384
            +QSAR VLD    PTRA YGVPED+FV CNFNQ+YK+DP  F+TWM++LKRV N+VLWLLRFPP  EANIR EAR RGV+++ +L FTDVA K+EH+KRGYLADLFLDTP CNAHTTGCDILW G
Sbjct:  716 KQSARDVLDTDKCPTRADYGVPEDKFVFCNFNQVYKIDPVTFTTWMNILKRVPNSVLWLLRFPPIAEANIRAEARARGVKDQTRLIFTDVAPKDEHLKRGYLADLFLDTPECNAHTTGCDILWGG 840
[Close]
BLAST of mRNA_E-siliculosus-1a_F_contig10951.659.1 vs. uniprot
Match: A0A2D4BUC4_PYTIN (Uncharacterized protein n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BUC4_PYTIN)

HSP 1 Score: 197 bits (500), Expect = 6.580e-56
Identity = 89/124 (71.77%), Postives = 105/124 (84.68%), Query Frame = 1 
Query:   13 QQSARYVLDRTLLPTRATYGVPEDRFVLCNFNQLYKMDPAIFSTWMSVLKRVSNAVLWLLRFPPAGEANIRMEARKRGVREEQLHFTDVATKEEHIKRGYLADLFLDTPSCNAHTTGCDILWSG 384
            +QSAR VLD    PTRA YGVPE++FV CNFNQ+YK+DPA F TWM++LKRV N++LWLLRFPP  EAN+R EAR +GV++ +LHFTDVA K+ H+KRGYLADLFLDTP CNAHTTGCDILW G
Sbjct:  733 KQSARDVLDVDKCPTRAHYGVPENQFVFCNFNQVYKIDPATFRTWMNILKRVPNSILWLLRFPPIAEANLRAEARAQGVKDSRLHFTDVAPKDXHLKRGYLADLFLDTPXCNAHTTGCDILWGG 856
[Close]
BLAST of mRNA_E-siliculosus-1a_F_contig10951.659.1 vs. uniprot
Match: A0A7S0XDQ2_9STRA (Hypothetical protein (Fragment) n=1 Tax=Chromulina nebulosa TaxID=96789 RepID=A0A7S0XDQ2_9STRA)

HSP 1 Score: 182 bits (463), Expect = 1.280e-55
Identity = 83/124 (66.94%), Postives = 99/124 (79.84%), Query Frame = 1 
Query:   13 QQSARYVLDRTLLPTRATYGVPEDRFVLCNFNQLYKMDPAIFSTWMSVLKRVSNAVLWLLRFPPAGEANIRMEARKRGVREEQLHFTDVATKEEHIKRGYLADLFLDTPSCNAHTTGCDILWSG 384
            +QS+R V+D+  +PTR  YG+ ED FV CNFNQLYK+DP+IF  WM +LKRV N++LWLLRFP AGE NI  EA  RGVR +Q+ FTDVA +EEH+KRG+L DLFLDTP CNAHTT CDILW G
Sbjct:   99 RQSSRDVVDKINMPTREQYGISEDAFVFCNFNQLYKIDPSIFDIWMRILKRVPNSILWLLRFPAAGEENIIREALARGVRRDQIIFTDVAPREEHVKRGFLPDLFLDTPVCNAHTTACDILWGG 222
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig10951.659.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KZU9_9PHAE1.920e-7698.39Protein O-GlcNAc transferase n=2 Tax=Ectocarpus Ta... [more]
A0A5D6YC07_9STRA2.600e-6074.19Uncharacterized protein (Fragment) n=1 Tax=Pythium... [more]
A0A7S2BCC6_9STRA2.850e-6076.19Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
K3X4T5_GLOUD4.650e-6072.58Uncharacterized protein n=1 Tax=Globisporangium ul... [more]
A0A2P4Y2W6_9STRA4.340e-5972.80UDP-N-acetylglucosamine-peptide N-acetylglucosamin... [more]
A0A8K1FGH6_PYTOL1.880e-5773.39Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
D0NXA5_PHYIT4.710e-5673.60Protein O-GlcNAc transferase n=2 Tax=Phytophthora ... [more]
A0A0W8DU52_PHYNI5.150e-5673.60Probable UDP-N-acetylglucosamine--peptide N-acetyl... [more]
A0A2D4BUC4_PYTIN6.580e-5671.77Uncharacterized protein n=1 Tax=Pythium insidiosum... [more]
A0A7S0XDQ2_9STRA1.280e-5566.94Hypothetical protein (Fragment) n=1 Tax=Chromulina... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig10951contigE-siliculosus-1a_F_contig10951:449..1443 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start1
Stop1
Cds size387
Model size387
Exons2
Hectar predicted targeting categoryother localisation
BRITEko00000,ko00001,ko01000,ko01003,ko03036
Best eggNOG OGNA|NA|NA
Best tax levelEukaryota
CAZyGT41
COG Functional cat.O
EC2.4.1.255
EggNOG OGsCOG3914@1,KOG4626@2759
EggNOG free text desc.protein N-acetylglucosaminyltransferase activity
GOsGO:0003674,GO:0003824,GO:0006464,GO:0006486,GO:0006493,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009100,GO:0009101,GO:0009987,GO:0016740,GO:0016757,GO:0019538,GO:0034645,GO:0036211,GO:0043170,GO:0043412,GO:0043413,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0070085,GO:0071704,GO:1901135,GO:1901137,GO:1901564,GO:1901566,GO:1901576
KEGG Pathwayko00514,ko04931,map00514,map04931
KEGG ReactionR09304,R09676
KEGG koko:K09667
KEGG rclassRC00005,RC00059
Seed eggNOG ortholog2880.D7FX68
Seed ortholog evalue1.7e-66
Seed ortholog score258.5
Taxonomic scopeEukaryota
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927598.5373588-CDS-E-siliculosus-1a_F_contig10951:448..5741622927598.5373588-CDS-E-siliculosus-1a_F_contig10951:448..574Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10951 449..574 -
1696318604.372318-CDS-E-siliculosus-1a_F_contig10951:448..5741696318604.372318-CDS-E-siliculosus-1a_F_contig10951:448..574Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10951 449..574 -
1622927598.5485692-CDS-E-siliculosus-1a_F_contig10951:1182..14431622927598.5485692-CDS-E-siliculosus-1a_F_contig10951:1182..1443Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10951 1183..1443 -
1696318604.3867033-CDS-E-siliculosus-1a_F_contig10951:1182..14431696318604.3867033-CDS-E-siliculosus-1a_F_contig10951:1182..1443Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10951 1183..1443 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig10951.659.1prot_E-siliculosus-1a_F_contig10951.659.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig10951 449..1443 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig10951.659.1

>prot_E-siliculosus-1a_F_contig10951.659.1 ID=prot_E-siliculosus-1a_F_contig10951.659.1|Name=mRNA_E-siliculosus-1a_F_contig10951.659.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=129bp
MLSRQQSARYVLDRTLLPTRATYGVPEDRFVLCNFNQLYKMDPAIFSTWM
SVLKRVSNAVLWLLRFPPAGEANIRMEARKRGVREEQLHFTDVATKEEHI
KRGYLADLFLDTPSCNAHTTGCDILWSG*
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mRNA from alignment at E-siliculosus-1a_F_contig10951:449..1443-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig10951.659.1 ID=mRNA_E-siliculosus-1a_F_contig10951.659.1|Name=mRNA_E-siliculosus-1a_F_contig10951.659.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=995bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig10951:449..1443- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATGCTTTCCCGCCAACAGTCGGCTAGGTACGTCCTGGATCGGACGCTCCT
CCCTACCCGAGCCACGTACGGCGTTCCGGAGGACCGTTTCGTGCTGTGCA
ACTTCAACCAGCTCTACAAGATGGACCCGGCTATCTTCTCCACCTGGATG
TCGGTGCTCAAGAGGGTGTCAAACGCGGTTCTGTGGCTGCTGAGGTTCCC
TCCGGCGGGGGAGGCGAACATCAGGATGGAGGCCAGGAAGAGGGGCGTCA
GGGAGGAGCAGGTGTCTTTGTGTGTGTGTGTGTGATTGTGTGTGTGTGTT
TTTTTTCCATGGACCGCGCTAACCGGCTTACCAATGAAAAGACGCCAGTC
TCAGTGCGGGAGGAAACAGTGTTCGTGTGTGTTTTGTCATCCCAACTATA
CTCTGGACGCCGGTCCTTACACCATTGATAGAATTTCTGGACACACCAGG
GCAGGAGGAGGTCGCACAGGATGTATGGGTGAAGGCTTTAATTTCAAGTA
TTGCTGCTGTTGCTCTTGTTGATATCGTCGCCTCGACGTTTACCGGCCAA
TTTTCGACTCTCCCATGTTCGTGCAGTTGGAGGTTACGAAGTCACGTCTG
GGTGATGATGATGTGTGGGGCACCTTCCTTGTGTTGGGCCAGCTCCGTAC
CTGTCATATTTGATCCACCTGTCCTTTGTTGTTGAGATTTCTGACAATTA
CGTCGCTCTGCTAACGACCCACCTCTTTTCTAGACACGCAGGTCCAACAA
TGTATATCACCAGCAACCGCAAAGCGTCTTGGGATTTGTGATATCCTCTC
TCTCCCTCCCTCTTTGCCACTAATCCCTCCCTCTCTTCCCGTCTTTTCCT
AATATTTTATATGGTACAGCTGCACTTCACAGACGTGGCTACGAAGGAGG
AGCACATCAAGCGGGGTTACCTGGCCGACCTCTTTCTCGACACCCCGAGC
TGCAACGCGCACACCACGGGCTGCGACATCCTGTGGAGCGGGTGA
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig10951:449..1443-

>mRNA_E-siliculosus-1a_F_contig10951.659.1 ID=mRNA_E-siliculosus-1a_F_contig10951.659.1|Name=mRNA_E-siliculosus-1a_F_contig10951.659.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=774bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig10951:449..1443- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATGCTTTCCCGCCAACAGTCGGCTAGGTACGTCCTGGATCGGACGCTCCT
CCCTACCCGAGCCACGTACGGCGTTCCGGAGGACCGTTTCGTGCTGTGCA
ACTTCAACCAGCTCTACAAGATGGACCCGGCTATCTTCTCCACCTGGATG
TCGGTGCTCAAGAGGGTGTCAAACGCGGTTCTGTGGCTGCTGAGGTTCCC
TCCGGCGGGGGAGGCGAACATCAGGATGGAGGCCAGGAAGAGGGGCGTCA
GGGAGGAGCAGATGCTTTCCCGCCAACAGTCGGCTAGGTACGTCCTGGAT
CGGACGCTCCTCCCTACCCGAGCCACGTACGGCGTTCCGGAGGACCGTTT
CGTGCTGTGCAACTTCAACCAGCTCTACAAGATGGACCCGGCTATCTTCT
CCACCTGGATGTCGGTGCTCAAGAGGGTGTCAAACGCGGTTCTGTGGCTG
CTGAGGTTCCCTCCGGCGGGGGAGGCGAACATCAGGATGGAGGCCAGGAA
GAGGGGCGTCAGGGAGGAGCAGCTGCACTTCACAGACGTGGCTACGAAGG
AGGAGCACATCAAGCGGGGTTACCTGGCCGACCTCTTTCTCGACACCCCG
AGCTGCAACGCGCACACCACGGGCTGCGACATCCTGTGGAGCGGGTGACT
GCACTTCACAGACGTGGCTACGAAGGAGGAGCACATCAAGCGGGGTTACC
TGGCCGACCTCTTTCTCGACACCCCGAGCTGCAACGCGCACACCACGGGC
TGCGACATCCTGTGGAGCGGGTGA
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