prot_E-siliculosus-1a_M_contig98.17730.1 (polypeptide) Ectocarpus siliculosus Ec864m_EcPH12_78m male

BLAST of mRNA_E-siliculosus-1a_M_contig98.17730.1 vs. uniprot
Match: A0A6H5KFS2_9PHAE (DNA topoisomerase 2 n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KFS2_9PHAE)

HSP 1 Score: 2649 bits (6866), Expect = 0.000e+0
Identity = 1549/1735 (89.28%), Postives = 1564/1735 (90.14%), Query Frame = 0

Query:   69 MSQEMWVYDEVADSIVKRAVTFVPGLYKIFDEIIVNAADNKQRDENMSKLKVTVDAESGKISVWNDGAGIPIQMHKEHHIYVPELIFGNLLTGSNFDDDQKKTTGGRNGFGAKLANIYSTEFVVETADRDAGKHYRQFFRNNMQEKEAPEIDCDYEGPDFTCITFTPDWKRFHMTGMEADTIALLRKRVYDMAGCMGGYAGANVKVYLDNRRVKVSGFKDYMSMYEGMETPVIFESIGDRWEVGVAASADSVFQQVSYVNAICTVKGGTHVECVANQVINKLLPVLKRKHKAEVKGHFVKNHLCIFVNGLVENPAFNSQSKEALTSKDTLFGSVPKISDAGLKKLEKSEIVDRVLSWAKHKQNQQLNKKSGKKSSKLTGITKLDDANHAGTAKSQDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLKGKLLNVREATGPQVLKNEEIQNVVKILGLKFNHKYDDTKQLRYGHLMIMTDQDHDGSHIKGLLINFVHHYWPSLLKIDGFLQEFITPIVKVSKGRNELSFYTVPEYTVWKEGHANGKGWNAKYYKGLGTSTAKEAKEYFAALDTHQLEFEWQDDDRDDDLIDMAFSKKRVEDRKEWLRGFKAGTHADYNVESVTYESFINKELILFSMEDNKRSIPCLVDGFKPSQRKVLFSCFKRNLKKEIKVVQLAGYVSEHSAYHHGETSLNGTITAMAQNFTGSNNLNLLTPGGQFGTRLMGGKDAASPRYIFTALQKAARAVFHPDDDALLTYLDDDGLSIEPEFYMPIVPLILVNGSEGIGTGWSSNVPNYSPHDVIENLKRRIKGDQFVEMQPWYKGFAGSIEAKVGKKGFEGSYTVKGIVNRVDDTTVEITELPVRKWTQDYKQFLESLLPGAEDAKSKDAKGNXXXXXXXXXXXXXXKSPTRCIKDFKENHTDTTVHFTVTMSMEDLDALEKGDGLYKFFKLESSVSTNNMVLFDQDGHLERYQSPQHIMEEFITLRSEFYGRRKTSLLNKLQSEWSKLDNKVRFILAVVEDRMQVNNRKKSELLEQLKREGYDLFLPATSKLRVAGGAEDGEGEDGHEESAADDSDTLSKGYDYLLGMKIWSLTLERVKLLENELEAKAEEMRILKATAPSELWLKDLDALGDVLDEIDEIIRKQEVEDAKQRKNAGKRKGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTRAPVPELVLCSSGGKVSPAFGHAESTGKFFTVVKKSDEDVGSLDGDDDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDPFDDDDVTAPTRQLAALTLADGAAKTAKGKKAKAAEDXXXXXXXXXXXXXXXXXMSLAARVKHKFGDTTTPAPKLPAKXXXXXXXXXXXXXXXXXXXXXXXXXFHGME-----------DEEVVAEPVVKPSRPGRAAAKNRKPIVDVISDXXXXXXEAGFDEDMGDSDDFHSAEEIDSDDGYEPTPAKARKPSKAKGKPAAEPAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-DGDSDGCEVVEAEPAVAPVRGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKGKGGSRGKAAASKAAATTTPAKSKGVKRGKRNSLSDEGDVFSPEKMSPPMKRASQKSTPAASRLRANKPLADDSDXXXXXXXXXXXXXXVEVTKTPRPARRAGRTQKVVYAELDS 1791
            MSQEMWVYDEVADSIVKRAVTFVPGLYKIFDEIIVNAADNKQRDENMSKLKVT+DAESGKISVWNDGAGIPIQMHKEHHIYVPELIFGNLLTGSNFDDDQKKTTGGRNGFGAKLANIYSTEFVVETADRDAGKHYRQ FRNNMQEK+APEIDCDYEGPDFTCITFTPDWKRFHMTGME+DTIALLRKRVYDMAGCMGGYAGA VKVYLDNRRVKVSGFKDYMSMYEGMETPVIFESIGDRWEVGVAASADSVFQQVSYVNAICTVKGGTHVECVANQVINKLLPVLKRKHKAEVKGHFVKNHLC+FVNGLVENPAFNSQSKEALTSKDTLFGSVPKISDAGLKKLEKSEIVDRVLSWAKHKQNQQLNKKSGKKSSKLTGITKLDDANHAGTAKSQDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLKGKLLNVREATGPQVLKNEEIQNVVKILGLKFNHKYDDTKQLRYGHLMIMTDQDHDGSHIKGLLINFVHHYWPSLLKIDGFLQEFITPIVKVSKGRNELSFYTVPEYTVWKEGHANGKGWNAKYYKGLGTSTAKEAKEYFAALDTHQLEFEWQDD RDDDLIDMAFSKKRVEDRKEWLRGFKAGTHADYNVESVTYESFINKELILFSMEDNKRSIPCLVDGFKPSQRKVLFSCFKRNL+KEIKVVQLAGYVSEHSAYHHGE SLNGTITAMAQNFTGSNNLNLLTPGGQFGTRLMGGKDAASPRYIFTAL+KAARAVFHPDDDALLTYLDDDGLSIEPEFYMPI+PLILVNGS+GIGTGWSSNVPNYSPHDVIENLKRRIKGDQFVEMQPWYKGFAGSIEAKVGKKGFEGSYTV+GIVNRVDDTTVEITELPVRKWTQDYKQFLESLLPGAEDAKSKDAKGNXXXXXXXXXXXXXXKSPTRCIKDFKENHTDTTVHFTVTMSMEDLDAL+KGDGLYKFFKLESSVSTNNMVLFDQDGHLERY+SPQHIMEEFITLRSEFY RRKTSLLNKLQSEWSKLDNKVRFILAVVEDRMQVNNRKKSELLEQLKREGYDLFLPATSKLRVAG  EDGEGEDG E   ADD DTLSKGYDYLLGMKIWSLTLERVKLLENELEAK EEMRILKATAPSELWLKDLDALGDVLDEIDEIIRKQEVEDAKQRKNAGKRKGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTRAPVPELV CSSGGKVSPAFG AES GKFF VVKKSDEDVGSL GD D                             DPFDDD VTAPTRQLAALT+ADGAAK AKGKKAKAAE XXXXXXXXXXXXXXXXXMSLAARVKHKFGDTTTPAPKLPAK XXXXXXXXX               FHGME           DEEVVAEPVVKPSRPGRAAAKNRKPIVDV+SD      EAGFDEDMGDSDDFHSAEE DSDDGYEPTPAKARKPSKAKG+PAAEP XXXXXXXXXXXXX                                    DGDSDGCEVVEAEPAVAPVRG                              XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX                   TPAKSKGVKRGKRNSLSDEGDVFSP+KMSPPMKRASQKSTPAASRLRANKPLADDSDXXXXXXXXXXXXXXVEVTKTPRPARRAGRTQKVVYAELDS
Sbjct:    1 MSQEMWVYDEVADSIVKRAVTFVPGLYKIFDEIIVNAADNKQRDENMSKLKVTLDAESGKISVWNDGAGIPIQMHKEHHIYVPELIFGNLLTGSNFDDDQKKTTGGRNGFGAKLANIYSTEFVVETADRDAGKHYRQVFRNNMQEKQAPEIDCDYEGPDFTCITFTPDWKRFHMTGMESDTIALLRKRVYDMAGCMGGYAGATVKVYLDNRRVKVSGFKDYMSMYEGMETPVIFESIGDRWEVGVAASADSVFQQVSYVNAICTVKGGTHVECVANQVINKLLPVLKRKHKAEVKGHFVKNHLCVFVNGLVENPAFNSQSKEALTSKDTLFGSVPKISDAGLKKLEKSEIVDRVLSWAKHKQNQQLNKKSGKKSSKLTGITKLDDANHAGTAKSQDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLKGKLLNVREATGPQVLKNEEIQNVVKILGLKFNHKYDDTKQLRYGHLMIMTDQDHDGSHIKGLLINFVHHYWPSLLKIDGFLQEFITPIVKVSKGRNELSFYTVPEYTVWKEGHANGKGWNAKYYKGLGTSTAKEAKEYFAALDTHQLEFEWQDDHRDDDLIDMAFSKKRVEDRKEWLRGFKAGTHADYNVESVTYESFINKELILFSMEDNKRSIPCLVDGFKPSQRKVLFSCFKRNLRKEIKVVQLAGYVSEHSAYHHGEASLNGTITAMAQNFTGSNNLNLLTPGGQFGTRLMGGKDAASPRYIFTALEKAARAVFHPDDDALLTYLDDDGLSIEPEFYMPIIPLILVNGSDGIGTGWSSNVPNYSPHDVIENLKRRIKGDQFVEMQPWYKGFAGSIEAKVGKKGFEGSYTVRGIVNRVDDTTVEITELPVRKWTQDYKQFLESLLPGAEDAKSKDAKGNXXXXXXXXXXXXXXKSPTRCIKDFKENHTDTTVHFTVTMSMEDLDALDKGDGLYKFFKLESSVSTNNMVLFDQDGHLERYESPQHIMEEFITLRSEFYDRRKTSLLNKLQSEWSKLDNKVRFILAVVEDRMQVNNRKKSELLEQLKREGYDLFLPATSKLRVAGSVEDGEGEDGGE---ADDGDTLSKGYDYLLGMKIWSLTLERVKLLENELEAKTEEMRILKATAPSELWLKDLDALGDVLDEIDEIIRKQEVEDAKQRKNAGKRKGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTRAPVPELVFCSSGGKVSPAFGQAESMGKFFAVVKKSDEDVGSLGGDGDDGDHGMDPGGFTGSGGSGHGDQDDSNGDDDPFDDDGVTAPTRQLAALTIADGAAKPAKGKKAKAAEXXXXXXXXXXXXXXXXXXMSLAARVKHKFGDTTTPAPKLPAKPXXXXXXXXXSTYVDSSDNSGSDDDFHGMESVRVRTDGLFQDEEVVAEPVVKPSRPGRAAAKNRKPIVDVMSDDEEEE-EAGFDEDMGDSDDFHSAEENDSDDGYEPTPAKARKPSKAKGRPAAEPXXXXXXXXXXXXXXVETKRGAGGVRGARGKTAVAAPEATTMLSSDHEEEEDGDSDGCEVVEAEPAVAPVRGGRGGRATAGKGRAAAKVTLDSDEEDDVFAMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTPAKSKGVKRGKRNSLSDEGDVFSPQKMSPPMKRASQKSTPAASRLRANKPLADDSDXXXXXXXXXXXXXXVEVTKTPRPARRAGRTQKVVYAELDS 1731          

BLAST of mRNA_E-siliculosus-1a_M_contig98.17730.1 vs. uniprot
Match: W7TC60_9STRA (DNA topoisomerase 2 n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TC60_9STRA)

HSP 1 Score: 1437 bits (3721), Expect = 0.000e+0
Identity = 725/1210 (59.92%), Postives = 908/1210 (75.04%), Query Frame = 0

Query:    5 ENRPLGISKGSKAKGGK------KAPLGSSRGGGANSNGGGQTVEQRYQKKTQLEHILLRPDTYIGSTEKMSQEMWVYDEVADSIVKRAVTFVPGLYKIFDEIIVNAADNKQRDENMSKLKVTVDAESGKISVWNDGAGIPIQMHKEHHIYVPELIFGNLLTGSNFDDDQKKTTGGRNGFGAKLANIYSTEFVVETADRDAGKHYRQFFRNNMQEKEAPEI-DCDYEGPDFTCITFTPDWKRFHMTGMEADTIALLRKRVYDMAGCMGGYAGANVKVYLDNRRVKVSGFKDYMSMYEGMETPVIFESIGDRWEVGVAASADSVFQQVSYVNAICTVKGGTHVECVANQVINKLLPVLKRKHKA-EVKGHFVKNHLCIFVNGLVENPAFNSQSKEALTSKDTLFGSVPKISDAGLKKLEKSEIVDRVLSWAKHKQNQQLNKKSGKKSSKLTGITKLDDANHAGTAKSQDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLKGKLLNVREATGPQVLKNEEIQNVVKILGLKFNHKYDDTKQLRYGHLMIMTDQDHDGSHIKGLLINFVHHYWPSLLKIDGFLQEFITPIVKVSKGRNEL-SFYTVPEYTVWKEGHANGKGWNAKYYKGLGTSTAKEAKEYFAALDTHQLEFEWQDDDRDDDLIDMAFSKKRVEDRKEWLRGFKAGTHADYNVESVTYESFINKELILFSMEDNKRSIPCLVDGFKPSQRKVLFSCFKRNLKKEIKVVQLAGYVSEHSAYHHGETSLNGTITAMAQNFTGSNNLNLLTPGGQFGTRLMGGKDAASPRYIFTALQKAARAVFHPDDDALLTYLDDDGLSIEPEFYMPIVPLILVNGSEGIGTGWSSNVPNYSPHDVIENLKRRIKGDQFVEMQPWYKGFAGSIEAKVGKKGFEGSYTVKGIVNRVDDTTVEITELPVRKWTQDYKQFLESLLPGAEDAKSKDAKGNXXXXXXXXXXXXXXKSPTRCIKDFKENHTDTTVHFTVTMSMEDLDALEKGD-GLYKFFKLESSVSTNNMVLFDQDGHLERYQSPQHIMEEFITLRSEFYGRRKTSLLNKLQSEWSKLDNKVRFILAVVEDRMQVNNRKKSELLEQLKREGYDLFLPATSKLRVAGGAEDGEGEDGHEESAADDSDTLSKGYDYLLGMKIWSLTLERVKLLENELEAKAEEMRILKATAPSELWLKDLDALGDVLDE 1204
            EN P    KG+ AK         KAPLG S     N+   G+++EQ YQKKTQLEHILLRPDTYIGSTE ++Q MWV D+    +V+R ++FVPGLYKIFDEI+VNA+DNKQRD  M  +K+T+D +S  ISVWN+G+GIP+ +HKEH IYVPELIFGNLLTGSNFDD  KKTTGGRNG+GAKLANI+ST F ++TAD+ +GK ++Q FR+NM  +E PE+     +  ++TC+TF+PD  RF M  ++  T+ALL KR YD+AGC  G+ G  +KVYL+  R+ +  F DY+ +YEG++ P+ FE +  RWEVGV  S D  FQQVS+VN ICTVKGG HV  VA+QV++KL  ++K+K+K  EVKGH +KNHL ++VN L+ENPAF+SQ+KE LT++ + FGS   +SD  LK +EKS +VD V++WAK KQ++QL KK G K SK+ GITKLDDAN AGTA+S DCTLILTEGDSAKTLA+SGL VVGRD YGVFPLKGK LNVREA   Q++KNEEIQN+VKI+GL+F  +Y D K LRYGHLMIMTDQDHDGSHIKGLLIN +HH+WPSLLK+ GFLQEFITPIVKVSKG     +FYT+PEY  WKE      GW  KYYKGLGTSTA+EAKEYF+ L TH+++F W DD  D D+IDMAF+KKRVEDRKEWLRGF  GT+ DY VES++Y++F+N+ELILFSM DN+RSIP L+DG KP+QRKVLF+CFKRNLKKEIKV QLAGYVSEHSAYHHGE SLNGTI  MAQNF GSNN+  L P GQFGTR+MGGKDAASPRY+FT L++  RA+FHP+DD LL   ++DG  IEPE+Y P++P +LVNG EGIGTGWSS VPNY+P D++ N++R ++G++  EM PWY+GF G++EAK G++   GSY V G V RVDD TVE+TELPV+KWTQDYKQFLES+  GA D K                           IKD KE HTDT+V+F +T+S      L   D  L+K  KLESS++T+NM LFD +GH+ +Y++P  ++ EF  LR   Y +R+ +LL +L+ EW ++DNKVRFIL+VV+ R+ V+NRKK+ELL++L REGY+LF P       AGG ++ EGE G E         L KGYDYLL MK+WSLTLERV+ L  +L  KA E+  L+ T+ ++LWL+D+DAL   L++
Sbjct:    2 ENVPKAKKKGASAKASASTAATMKAPLGESSSS-INAGEEGRSIEQIYQKKTQLEHILLRPDTYIGSTESVTQPMWVLDDAQKKLVQRNISFVPGLYKIFDEIMVNASDNKQRDPTMDTIKITIDQDSNTISVWNNGSGIPVVVHKEHQIYVPELIFGNLLTGSNFDDGMKKTTGGRNGYGAKLANIFSTRFTIQTADKASGKKFKQTFRDNMTVREEPEVVSMGSKEGEYTCVTFSPDLTRFKMDRLDGATVALLAKRAYDVAGCASGFPGKPLKVYLNEERLSLPTFLDYLKLYEGLDAPIAFERVNPRWEVGVGIS-DGQFQQVSFVNGICTVKGGQHVNFVADQVVSKLTALVKKKNKGQEVKGHQIKNHLVVYVNALIENPAFDSQTKETLTTRPSSFGSSCSVSDKFLKTVEKSSLVDSVVNWAKFKQDEQLKKKGGSKKSKVLGITKLDDANFAGTARSSDCTLILTEGDSAKTLAISGLSVVGRDYYGVFPLKGKPLNVREAAHAQIVKNEEIQNIVKIMGLRFGTQYTDAKSLRYGHLMIMTDQDHDGSHIKGLLINLLHHFWPSLLKLPGFLQEFITPIVKVSKGSKRADAFYTIPEYEQWKERTNEAAGWTIKYYKGLGTSTAQEAKEYFSDLSTHRIDFCWADDG-DGDVIDMAFAKKRVEDRKEWLRGFSKGTYVDYKVESMSYDTFVNQELILFSMADNQRSIPHLMDGLKPAQRKVLFACFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLNGTIVGMAQNFVGSNNIGFLYPSGQFGTRIMGGKDAASPRYVFTRLEEVTRALFHPEDDQLLPLQEEDGQVIEPEYYAPVIPTVLVNGGEGIGTGWSSTVPNYNPRDIMANIRRLLRGEEPEEMHPWYRGFTGTVEAKGGREA--GSYAVLGTVRRVDDVTVEVTELPVKKWTQDYKQFLESMTVGASDEKGA------------------------FIKDLKERHTDTSVYFVITLSETAAPELLVDDQALFKRLKLESSITTSNMHLFDAEGHIVKYETPLAVLREFYPLRLSLYEKRRAALLQRLEREWRRMDNKVRFILSVVDGRLVVSNRKKAELLQELAREGYELFAPG------AGGYDEEEGEGGGE---------LGKGYDYLLSMKLWSLTLERVEDLRGQLAQKAAELEALRGTSAADLWLQDMDALEQKLED 1167          

BLAST of mRNA_E-siliculosus-1a_M_contig98.17730.1 vs. uniprot
Match: A0A835YRC0_9STRA (DNA topoisomerase 2 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YRC0_9STRA)

HSP 1 Score: 1401 bits (3626), Expect = 0.000e+0
Identity = 717/1138 (63.01%), Postives = 860/1138 (75.57%), Query Frame = 0

Query:   73 MWVYDEVADSIVKRAVTFVPGLYKIFDEIIVNAADNKQRDENMSKLKVTVDAESGKISVWNDGAGIPIQMHKEHHIYVPELIFGNLLTGSNFDDDQKKTTGGRNGFGAKLANIYSTEFVVETADRDAGKHYRQFFRNNMQEKEAPEIDCDYEGPDFTCITFTPDWKRFHMTGMEADTIALLRKRVYDMAGCMGGYAGANVKVYLDNRRVKVSGFKDYMSMYEGMETPVIFESIGDRWEVGVAASADSVFQQVSYVNAICTVKGGTHVECVANQVINKLLPVLKRKHKA-EVKGHFVKNHLCIFVNGLVENPAFNSQSKEALTSKDTLFGSVP-KISDAGLKKLEKSEIVDRVLSWAKHKQNQQLNKKSGKKSSKLTGITKLDDANHAGTAKSQDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLKGKLLNVREATGPQVLKNEEIQNVVKILGLKFNHKYDDTKQLRYGHLMIMTDQDHDGSHIKGLLINFVHHYWPSLLKIDGFLQEFITPIVKVSKGRNELSFYTVPEYTVWKEGHANGKGWNAKYYKGLGTSTAKEAKEYFAALDTHQLEFEWQDDDRDDDLIDMAFSKKRVEDRKEWLRGFKAGTHADYNVESVTYESFINKELILFSMEDNKRSIPCLVDGFKPSQRKVLFSCFKRNLKKEIKVVQLAGYVSEHSAYHHGETSLNGTITAMAQNFTGSNNLNLLTPGGQFGTRLMGGKDAASPRYIFTALQKAARAVFHPDDDALLTYLDDDGLSIEPEFYMPIVPLILVNGSEGIGTGWSSNVPNYSPHDVIENLKRRIKGDQFVEMQPWYKGFAGSIEAKVGKKGFEGS-YTVKGIVNRVDDTTVEITELPVRKWTQDYKQFLESLLPGAEDAKSKDAKGNXXXXXXXXXXXXXXKSPTRCIKDFKENHTDTTVHFTVTMSMEDLDALEKGDG-LYKFFKLESSVSTNNMVLFDQDGHLERYQSPQHIMEEFITLRSEFYGRRKTSLLNKLQSEWSKLDNKVRFILAVVEDRMQVNNRKKSELLEQLKREGYDLFLPATSKLRVAGGAEDGEGEDGHEESAADDSD-----TLSKGYDYLLGMKIWSLTLERVKLLENELEAKAEEMRILKATAPSELWLKDLDALGDV 1201
            MWV+D     IV+R+V++VPGLYKIFDEIIVNAADNKQRD  M+ LKVTVD  +G ISVWN+G GIPI  H EH  Y+PELIFG+LLTGSNF+D +KKTTGGRNG+GAKLANI+STEFVVETAD    K YRQ FRNNM +K+ P I  DY+G +FTCITF PDW+RF M GME DT ALL+KR +D+AGCM  Y G  V+VYL+ +++ V  FKDY+ +YEG+   V+ E IG  W++ +  S D   QQVS+VNAICTVKGG HV  +A Q++  L+ V+KRK K  EVK   V+NHLC+ VN LVENPAF+SQSKE LT+K + FG    K+SD  LK +EKS IV+ +L++   + ++ L+KKSGKK+SKLTGITKLDDAN AGT  SQDCTLILTEGDSAKTLAVSGLGVVGRD YGVFPLKGK LNVRE     V KNEEI N+VKILGLKF  +Y D + LRYGHLMIMTDQDHDGSHIKGLLIN +H++WPSLL+++GFL EFITPIVK  +   ELSF+TVPEY  W+  HA  +GW+ KYYKGLGTSTAKEAKEYFA LD HQ+ FEW+DD  D D++D+ F+KKR EDRKEWLR F  GT  DY V+++TY++F N+ELILFS+EDNKRS+PC+VDG KPSQRKVLF+CFKR LK EIKV QLAGYVSEHSAYHHGE SL GTITAMAQ++ GSNN+ LL PGGQFGTRLMGGKD ASPRYIFT L K ARA+FHPDDDA+LTYLDDDG+SIEP +Y+P++PL+LVNG+EGIGTGWSS VPNYSP DV+ENL+RRI+G   VEMQPWY+GF G+I  K G  G +GS +TV+GIV R  D T+EITELPV+KWTQDYK FLES  P    AK                       P   IKDF+ENHTDTTVHFT+TM   DLDA+E+  G L KFFKLE++++  NM LFD  G + RY+SP  IMEEF  LR   YG RK  LL ++Q EW +LDNKVRF++AVV+ +++V NRKKSELL QL+ +G+D  LP ++   V   A+                       L+KGYDYLLG+K+W+LT E V  L  EL AK +EM  L+A   S+LWL+DLDA+G+V
Sbjct:    1 MWVFDADTSRIVRRSVSYVPGLYKIFDEIIVNAADNKQRDPTMTMLKVTVDKAAGSISVWNNGRGIPIARHAEHGCYIPELIFGHLLTGSNFNDQEKKTTGGRNGYGAKLANIFSTEFVVETADGATRKRYRQVFRNNMGDKDEPVIK-DYDGDEFTCITFKPDWRRFGMAGMEDDTEALLQKRAHDIAGCMSSYGGKRVRVYLNKQQLDVKSFKDYLGLYEGLSDLVLHERIG-TWDIAIGPS-DGQLQQVSFVNAICTVKGGQHVTHIAEQIVAHLVKVVKRKSKGTEVKPVHVRNHLCVCVNALVENPAFDSQSKETLTTKASAFGGADLKLSDKALKAIEKSPIVEAILAYTNFQASKALDKKSGKKTSKLTGITKLDDANFAGTKHSQDCTLILTEGDSAKTLAVSGLGVVGRDYYGVFPLKGKPLNVRETAHAAVSKNEEISNIVKILGLKFGTQYADARALRYGHLMIMTDQDHDGSHIKGLLINLLHYFWPSLLRVEGFLLEFITPIVKCVRAGRELSFFTVPEYETWRAAHAQQRGWSIKYYKGLGTSTAKEAKEYFAKLDQHQIPFEWRDDAADGDMVDLVFNKKRAEDRKEWLRNFVPGTFVDYGVDAMTYDAFFNRELILFSIEDNKRSLPCMVDGLKPSQRKVLFACFKRKLKNEIKVAQLAGYVSEHSAYHHGEVSLTGTITAMAQSYCGSNNVALLYPGGQFGTRLMGGKDQASPRYIFTRLDKVARAIFHPDDDAILTYLDDDGMSIEPAYYVPVLPLVLVNGAEGIGTGWSSGVPNYSPRDVMENLRRRIRGQPMVEMQPWYRGFVGTIARKAG--GAKGSSFTVEGIVERTGDETLEITELPVKKWTQDYKAFLES--PTKAAAK-----------------------PRFHIKDFRENHTDTTVHFTITMDKSDLDAIEQQPGGLSKFFKLETTLAETNMTLFDNTGMIARYESPLQIMEEFYDLRLRHYGMRKEHLLARMQKEWMRLDNKVRFVVAVVQGKLEVRNRKKSELLAQLRADGFDPLLPESAAKSVGSKAKXXXXXXXXXXXXXXXXXXXXXXALAKGYDYLLGLKLWTLTKEVVAKLLAELAAKKQEMEELRAMELSDLWLRDLDAVGEV 1108          

BLAST of mRNA_E-siliculosus-1a_M_contig98.17730.1 vs. uniprot
Match: K3X2K0_GLOUD (DNA topoisomerase 2 n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3X2K0_GLOUD)

HSP 1 Score: 1341 bits (3471), Expect = 0.000e+0
Identity = 682/1191 (57.26%), Postives = 877/1191 (73.64%), Query Frame = 0

Query:   40 QTVEQRYQKKTQLEHILLRPDTYIGSTEKMSQEMWVYDEVADSIVKRAVTFVPGLYKIFDEIIVNAADNKQRDENMSKLKVTVDAESGKISVWNDGAGIPIQMHKEHHIYVPELIFGNLLTGSNFDDDQKKTTGGRNGFGAKLANIYSTEFVVETADRDAGKHYRQFFRNNMQEKEAPEIDCDYEGPDFTCITFTPDWKRFHMTGMEADTIALLRKRVYDMAGCMGGYAGANVKVYLDNRRVKVSGFKDYMSMYEGMETP-----------------VIFESIGDRWEVGVAASADSVFQQVSYVNAICTVKGGTHVECVANQVINKLLPVLKRKHKAE-VKGHFVKNHLCIFVNGLVENPAFNSQSKEALTSKDTLFGSVPKISDAGLKKLEKSEIVDRVLSWAKHKQNQQLNKKSG--KKSSKLTGITKLDDANHAGTAKSQDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLKGKLLNVREATGPQVLKNEEIQNVVKILGLKFNHKYDDTKQLRYGHLMIMTDQDHDGSHIKGLLINFVHHYWPSLLKIDGFLQEFITPIVKVSKGRNELS-FYTVPEYTVWKEGHANGKGWNAKYYKGLGTSTAKEAKEYFAALDTHQLEFEWQDDDRDDDLIDMAFSKKRVEDRKEWLRGFKAGTHADYNVESVTYESFINKELILFSMEDNKRSIPCLVDGFKPSQRKVLFSCFKRNLKKEIKVVQLAGYVSEHSAYHHGETSLNGTITAMAQNFTGSNNLNLLTPGGQFGTRLMGGKDAASPRYIFTALQKAARAVFHPDDDALLTYLDDDGLSIEPEFYMPIVPLILVNGSEGIGTGWSSNVPNYSPHDVIENLKRRIKGDQFVEMQPWYKGFAGSI-EAKVGKKGFEGSYTVKGIVNRVDDTTVEITELPVRKWTQDYKQFLESLLPGAEDAKSKDAKGNXXXXXXXXXXXXXXKSPTRCIKDFKENHTDTTVHFTVTMSMEDL-DALEKGDGLYKFFKLESSVSTNNMVLFDQDGHLERYQSPQHIMEEFITLRSEFYGRRKTSLLNKLQSEWSKLDNKVRFILAVVEDRMQVNNRKKSELLEQLKREGYDLFLPATSKLRVAGGAEDGEGEDGHEESAADDSDTLSKGYDYLLGMKIWSLTLERVKLLENELEAKAEEMRILKATAPSELWLKDLDALGDVLDEIDE 1207
            Q++EQ YQKKTQLEHILLRPDTY+GS E ++Q MWV+D+    +  R +T+VPGLYKIFDEIIVNA DNKQRD+ M  LKV +DAE+G++SVWN+G GIP+ MHKEH++YVPELIFG+LLTGSNFDD +KKTTGGRNG+GAKLANI+S EF+VETAD +  + Y+Q F +NM +K  P I   +   DFTCITF PD  RF M+ ++ D +AL +KRVYD+AG        ++ VYL++ ++ +  F +Y+++Y+  + P                 VIF+    RW+VGVA S D  FQQVS+VN ICT KGG HV  + +Q+  KL+ V+K+K+K + VK +++KNH+ ++V+ L++NPAF+SQ+KE LT++ + FGS   +S+  LK++EKS +V+R+LS+A++KQ  +L +K G   K  +LTGI KLDDAN+AGT+K QDCTLI+TEGDSAKT+AV GL VVGRD YGVFPL+GKLLNVREA+  Q+LKNEEIQNVVKILGLK+  KY+ TK LRYGHLMIM DQDHDGSHIKGL+INF+HH+WPSLL+IDGF+QEFITPIVK +KG+N    FYT+PEY  W+E   NG+GW+ KYYKGLGTST +EAKEYF+ L THQ+ F + D D D D IDMAFSKKRVE+RK+WLRG++AGTH DY+V+ + Y  F+NKELILFSM DN RSIP +VDGFKPSQRKVLF CFKRNLK EIKV QLAGYVSEHSAYHHGE SLNGTI  MAQ+F GSNN++LL+P GQFGTRLMGGKDAASPRYIFT L++ AR +F P D+ +L YLDDDG SIEP+FY+PI+P+ LVNGSEGIGTGWSS++PNY+P D+I+NL++++ G+    + PWY+GF+G I E   GK     ++ V+G+    D++T+ I+ELPV+ WTQ YKQFLESLL                                  IKDF+ENHTD  V FT+T   + L D      G+ K FKLE+S+ST+NM LFD  GH+++Y++P  I+EEF  +R ++YGRRK +LL KL+ +   L NK RF+LAVV+  + V+NRKK +LLEQL  +GYD  L  T+K + +G        D  EES  +++   ++GYDYLL MKI SLT ERV+ L  EL+ + +E   L+AT   +LWL DLD L  VL E +E
Sbjct:   85 QSIEQIYQKKTQLEHILLRPDTYVGSIEPVTQNMWVFDDENKKMEMRKITYVPGLYKIFDEIIVNACDNKQRDKTMDTLKVKIDAENGEVSVWNNGKGIPVVMHKEHNVYVPELIFGHLLTGSNFDDKKKKTTGGRNGYGAKLANIFSNEFIVETADSNTKQRYKQVFESNMGKKNPPTI-TSWSKKDFTCITFKPDLARFKMSSLDDDIVALFKKRVYDVAGV----TDKSLNVYLNDEKIAIKSFPEYVNLYKDSKKPSNESGSGDEKDTPSSDAVIFDKPDGRWQVGVALSDDG-FQQVSFVNGICTTKGGQHVNYITDQITAKLIAVVKKKNKGDTVKPNYIKNHMTVYVSALIDNPAFDSQTKETLTTRSSGFGSTYTLSEKFLKQIEKSGMVERILSFAQYKQTAELRRKGGGGAKKVRLTGIAKLDDANNAGTSKGQDCTLIVTEGDSAKTIAVGGLSVVGRDYYGVFPLRGKLLNVREASHAQILKNEEIQNVVKILGLKYGQKYESTKSLRYGHLMIMADQDHDGSHIKGLVINFIHHFWPSLLQIDGFIQEFITPIVKCTKGKNNSKVFYTIPEYNAWREVTNNGRGWSIKYYKGLGTSTTQEAKEYFSDLKTHQVGFTY-DGDEDGDAIDMAFSKKRVEERKDWLRGYQAGTHVDYDVDDMAYSEFVNKELILFSMADNMRSIPSVVDGFKPSQRKVLFCCFKRNLKVEIKVAQLAGYVSEHSAYHHGEQSLNGTIINMAQDFVGSNNVHLLSPNGQFGTRLMGGKDAASPRYIFTNLEQVARRIFDPLDNDVLNYLDDDGQSIEPDFYVPIIPMALVNGSEGIGTGWSSSIPNYNPLDIIKNLRQKLNGEDLSPLVPWYRGFSGHIIEKSSGKPSDNQTFLVQGLYEIKDESTLVISELPVKTWTQTYKQFLESLLESGT------------------------------IKDFRENHTDAKVLFTITFEPKTLSDICSAPGGVVKKFKLETSISTSNMHLFDSTGHIKKYENPHEILEEFYAIRLDYYGRRKAALLRKLEEQIKILSNKTRFVLAVVDGSLVVSNRKKQDLLEQLVADGYDQVLAKTAKAKKSG--------DNDEESDEEEATDAARGYDYLLSMKILSLTKERVEKLRAELQDREQEHSKLEATTIEQLWLGDLDQLEAVLVETEE 1230          

BLAST of mRNA_E-siliculosus-1a_M_contig98.17730.1 vs. uniprot
Match: A0A6G0X127_9STRA (DNA topoisomerase 2 n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0X127_9STRA)

HSP 1 Score: 1331 bits (3444), Expect = 0.000e+0
Identity = 686/1163 (58.99%), Postives = 853/1163 (73.34%), Query Frame = 0

Query:   39 GQTVEQRYQKKTQLEHILLRPDTYIGSTEKMSQEMWVYDEVADSIVKRAVTFVPGLYKIFDEIIVNAADNKQRDENMSKLKVTVDAESGKISVWNDGAGIPIQMHKEHHIYVPELIFGNLLTGSNFDDDQKKTTGGRNGFGAKLANIYSTEFVVETADRDAGKHYRQFFRNNMQEKEAPEIDCDYEGPDFTCITFTPDWKRFHMTGMEADTIALLRKRVYDMAGCMGGYAGANVKVYLDNRRVKVSGFKDYMSMYEGMETPVIFESIGDRWEVGVAASADSVFQQVSYVNAICTVKGGTHVECVANQVINKLLPVLKRKHKAE-VKGHFVKNHLCIFVNGLVENPAFNSQSKEALTSKDTLFGSVPKISDAGLKKLEKSEIVDRVLSWAKHKQNQQLNKKSGKKSSKLTGITKLDDANHAGTAKSQDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLKGKLLNVREATGPQVLKNEEIQNVVKILGLKFNHKYDDTKQLRYGHLMIMTDQDHDGSHIKGLLINFVHHYWPSLLKIDGFLQEFITPIVKVSKGRNELSFYTVPEYTVWKEGHANGKGWNAKYYKGLGTSTAKEAKEYFAALDTHQLEFEWQDDDRDDDLIDMAFSKKRVEDRKEWLRGFKAGTHADYNVESVTYESFINKELILFSMEDNKRSIPCLVDGFKPSQRKVLFSCFKRNLKKEIKVVQLAGYVSEHSAYHHGETSLNGTITAMAQNFTGSNNLNLLTPGGQFGTRLMGGKDAASPRYIFTALQKAARAVFHPDDDALLTYLDDDGLSIEPEFYMPIVPLILVNGSEGIGTGWSSNVPNYSPHDVIENLKRRIKGDQFVEMQPWYKGFAGSIEAKVGKKGFEGSYTVKGIVNRVDDTTVEITELPVRKWTQDYKQFLESLLPGAEDAKSKDAKGNXXXXXXXXXXXXXXKSPTRCIKDFKENHTDTTVHFTVTMSMEDLDALEKGDG-LYKFFKLESSVSTNNMVLFDQDGHLERYQSPQHIMEEFITLRSEFYGRRKTSLLNKLQSEWSKLDNKVRFILAVVEDRMQVNNRKKSELLEQLKREGYDLFLPATSKLRVAGGAEDGEGEDGHEESAADDSDTLSKGYDYLLGMKIWSLTLERVKLLENELEAKAEEMRILKATAPSELWLKDLDALG 1199
            G TVEQ YQKK+QLEHILLRPDTY+GS E + Q MWVYD+    +V + +T  PGLYKIFDEIIVNA DNKQRD +M  LKVT+DAE G+ISVWN+G GIP+ MHKEH++YVPELIFG+LLTGSNFDD++KKTTGGRNG+GAKLANI+S EFVVETA ++ GK YRQ F +NM  K  P+I   +   DFTCITF PD++RF MTG++ D ++L +KRVYD+AG         + VYL+  ++ V  F  Y+++Y G E  VIF+   +RW+VG+  S D  FQQVS+VN ICT KGG HV  +A+Q+ NKL+ V+K+K+K E VK  +VKNH+ ++V+ L++NPAF+SQ+KE LT++   FGS   +SD  LK +EKS +V+R+LS+AK KQ  +L K +G K +KLTGI+KLDDAN AG+AK QDCTLILTEGDSAK LA+SGL VVGRD YGVFPLKGKLLNVREA    +LKNEEIQN+VKILGLK+   Y+ TK LRYGHLMIM DQDHDGSHIKGL+INF+HH+WPSL+ +D F+QEFITPIVK +KG     FYT+PEY VW+    N KGW  KYYKGLGTS  +EA+EYF+ L+THQ+ F +  D  D D IDMAFSKKRVEDRKEWLRGF  GT+ DY V+ + Y  F+NKELILFSM DN RSIP +VDGFKPSQRKVLF+CFKRNLKKEIKV QL+GY+SEHSAYHHGE +L GTI  MAQNF GSNN+NLL+P GQFGTRLMGGKDAAS RYIFT L+   R +F+P DD +LTYL+D+G SIEP++YMP +P+ LVNGS+GIGTGWS  +PNY P  +IENL+RRI+ +    M P+Y+GF G+I  K     F     V+G+   VDD+T+ I+ELPV+ WTQ YKQFLE+LL                                  IKDFKENHTDTTV FTV ++ E L ++++  G + K FKLESS++T+NM LFD +G ++ Y SP  I+EEF  LR E+YG RK S+L KL+ E   L N+ RFILAV+E  + VNNRKK +LLE+L+++ +D   P   K      A+D E     +E+  D +D  SK YDYLL M +WSLT ERV+ L N+LEAK  E   + AT+  ++WLKDLD L 
Sbjct:   25 GLTVEQIYQKKSQLEHILLRPDTYVGSIEPVQQAMWVYDDEEKKMVLKKITICPGLYKIFDEIIVNACDNKQRDSSMDTLKVTIDAEKGEISVWNNGRGIPVVMHKEHNVYVPELIFGHLLTGSNFDDNKKKTTGGRNGYGAKLANIFSKEFVVETASQEHGKRYRQVFSDNMGVKGTPKI-TSWSKKDFTCITFRPDFERFQMTGLDEDIVSLFKKRVYDIAGV----TDKTLNVYLNEEKISVKSFSQYVALY-GSED-VIFDKPDERWQVGLGMSDDG-FQQVSFVNGICTTKGGQHVNYLADQIANKLIAVVKKKNKGEAVKPAYVKNHMFLYVSALIDNPAFDSQTKETLTTRPPSFGSTYTLSDKFLKAVEKSGLVERILSFAKFKQTAELKKTAGGKRTKLTGISKLDDANFAGSAKGQDCTLILTEGDSAKALAMSGLAVVGRDYYGVFPLKGKLLNVREAAHSAILKNEEIQNIVKILGLKYGQTYESTKSLRYGHLMIMADQDHDGSHIKGLVINFIHHFWPSLMGLDNFVQEFITPIVKATKGSRSEVFYTIPEYEVWRNQMNNAKGWYIKYYKGLGTSKPEEAREYFSDLNTHQIGFTYTSD-ADGDAIDMAFSKKRVEDRKEWLRGFVPGTYVDYAVQDMPYTEFVNKELILFSMADNVRSIPSMVDGFKPSQRKVLFACFKRNLKKEIKVAQLSGYISEHSAYHHGEVNLQGTIVGMAQNFVGSNNINLLSPNGQFGTRLMGGKDAASARYIFTNLEPITRLIFNPLDDGVLTYLEDEGQSIEPQWYMPTIPMALVNGSDGIGTGWSCGIPNYDPLAIIENLRRRIQAEPMEPMSPFYRGFKGTIVQKSSSDNF----LVQGVYEVVDDSTIVISELPVKSWTQTYKQFLETLLEAG------------------------------VIKDFKENHTDTTVLFTVNLTPESLASIQQAPGGIVKKFKLESSLATSNMHLFDSNGQIKHYNSPLDIIEEFYALRLEYYGLRKASILKKLEHEILVLSNRARFILAVIEGSLIVNNRKKKDLLEELRQKKFD---PMPKKPAPIVAADDNE----PQEADDDANDVSSKDYDYLLSMPLWSLTAERVESLRNDLEAKNNERDSIFATSLEQMWLKDLDDLA 1137          

BLAST of mRNA_E-siliculosus-1a_M_contig98.17730.1 vs. uniprot
Match: A0A024G308_9STRA (DNA topoisomerase 2 n=2 Tax=Albugo candida TaxID=65357 RepID=A0A024G308_9STRA)

HSP 1 Score: 1327 bits (3435), Expect = 0.000e+0
Identity = 673/1182 (56.94%), Postives = 877/1182 (74.20%), Query Frame = 0

Query:   40 QTVEQRYQKKTQLEHILLRPDTYIGSTEKMSQEMWVYDEVADSIVKRAVTFVPGLYKIFDEIIVNAADNKQRDENMSKLKVTVDAESGKISVWNDGAGIPIQMHKEHHIYVPELIFGNLLTGSNFDDDQKKTTGGRNGFGAKLANIYSTEFVVETADRDAGKHYRQFFRNNMQEKEAPEIDCDYEGPDFTCITFTPDWKRFHMTGMEADTIALLRKRVYDMAGCMGGYAGANVKVYLDNRRVKVSGFKDYMSMY--EGMETPVIFESIGDRWEVGVAASADSVFQQVSYVNAICTVKGGTHVECVANQVINKLLPVLKRKHKAE-VKGHFVKNHLCIFVNGLVENPAFNSQ--SKEALTSKDTLFGSVPKISDAGLKKLEKSEIVDRVLSWAKHKQNQQLNKKSGK--KSSKLTGITKLDDANHAGTAKSQDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLKGKLLNVREATGPQVLKNEEIQNVVKILGLKFNHKYDDTKQLRYGHLMIMTDQDHDGSHIKGLLINFVHHYWPSLLKIDGFLQEFITPIVKVSKGRNELSFYTVPEYTVWKEGHANGKGWNAKYYKGLGTSTAKEAKEYFAALDTHQLEFEWQDDDRDDDLIDMAFSKKRVEDRKEWLRGFKAGTHADYNVESVTYESFINKELILFSMEDNKRSIPCLVDGFKPSQRKVLFSCFKRNLKKEIKVVQLAGYVSEHSAYHHGETSLNGTITAMAQNFTGSNNLNLLTPGGQFGTRLMGGKDAASPRYIFTALQKAARAVFHPDDDALLTYLDDDGLSIEPEFYMPIVPLILVNGSEGIGTGWSSNVPNYSPHDVIENLKRRIKGDQFVE-MQPWYKGFAGSIEAKVGKK-GFEGSYTVKGIVNRVDDTTVEITELPVRKWTQDYKQFLESLLPGAEDAKSKDAKGNXXXXXXXXXXXXXXKSPTRCIKDFKENHTDTTVHFTVTMSMEDL-DALEKGDGLYKFFKLESSVSTNNMVLFDQDGHLERYQSPQHIMEEFITLRSEFYGRRKTSLLNKLQSEWSKLDNKVRFILAVVEDRMQVNNRKKSELLEQLKREGYDLFLPATSKLRVAGGAEDGEGEDGHEESAADDSDTLSKGYDYLLGMKIWSLTLERVKLLENELEAKAEEMRILKATAPSELWLKDLDALGDVLDEIDEIIRK 1211
            QT+EQ YQKKTQL+HILLRPDTY+GS + ++Q MW+YDE +  +  + +T+VPGLYKIFDEIIVNA DNKQRD +M+ ++VT+D  +G++SVWN+G GIP+  HKEHH+YVPELIFG+LLTGSNFDD +KKTTGGRNG+GAKLANI+ST FVVETAD++  K Y+Q F +NM +KEAP I   Y   DFTCITF PD KRF +  ++ D ++L +KRV+D+AG        +++VYL+  ++    F DY+S+Y  +  +  VIF+   +RW+VG   S D  FQQ+S+VN ICT +GG HV  VA+QV+NKL+  +K+++K E VK +++KN + I+V+ L++NP+F+SQ  +KE LT++   FGS   +SD  LK++EKS IV+RVLS+AK KQ  +L +K GK    SKLTGITKLDDAN+AGTA+ ++CTLILTEGDSAK+L + GL VVGRD YGVFPLKGKLLNVREA+  Q+LKNEEIQN+VKILGLK+   Y  TK LRYGHLMIM DQDHDGSHIKGL+INF+HH+WPSLL+I+GF+ EFITPIVK +KGR +  FYT+PEY  W+    NG+GW  KYYKGLGTST +EAKEYF+ L THQ++F ++ ++ D D IDMAFSKKRVE+RK+WLRG+  GTH DYNV+ + Y  F+NKELILFS  DN RSIPCLVDGFKPSQRKVL+ CFKRNLK EIKV QLAGYVSEHS+YHHGE SL+GTI  MAQ+FTGSNN NLL+P GQFGTR++GGKDAASPRYIFT L+  A  +F P+DD+LL +LD+DG  IEPE+Y+P++P++LVNGSEGIGTGWSS++PNY+P D+IENL+ RIKG++    + PWY+GF GSIE +   + G   ++ V+GI +   D+T+ ITELPV+ WTQ YKQFLE+LL                                  IKDFKENHTD+ V FT++M+ E L + ++   G+ K FKLE+S+S +NM LFD DG +++Y SP  I+  F  +R  +YG+RK ++LNKL  +   L NKV+FILAV+   + V+NRKK ELL++L   GYD   P +SK++     ++ + E+  +E   D +  L+KGY+YLL M IWSLT ERV+ L  EL+ +  E + L A +  ELWL DL+ L  +L + +EI +K
Sbjct:   70 QTIEQIYQKKTQLQHILLRPDTYVGSIDPVTQNMWIYDEESSKMQYKKITYVPGLYKIFDEIIVNACDNKQRDPSMNTVRVTIDPLAGEVSVWNNGKGIPVVQHKEHHVYVPELIFGHLLTGSNFDDKKKKTTGGRNGYGAKLANIFSTRFVVETADKNVRKRYKQVFSDNMSQKEAPSI-TSYNKQDFTCITFRPDLKRFKLDRLDDDIVSLFKKRVFDIAGI----TDKSLQVYLNEEKITCKSFPDYISLYTSDSAKEDVIFDKPTERWQVGFGPSDDG-FQQISFVNGICTTRGGQHVTYVADQVVNKLVATVKKRNKGEPVKPNYIKNMMTIYVSALIDNPSFDSQVSTKETLTTRPASFGSAHTLSDKFLKQIEKSGIVERVLSFAKFKQTAELQRKGGKGGNRSKLTGITKLDDANYAGTAEGKNCTLILTEGDSAKSLTIGGLSVVGRDYYGVFPLKGKLLNVREASHSQILKNEEIQNIVKILGLKYGQVYQSTKSLRYGHLMIMADQDHDGSHIKGLVINFIHHFWPSLLQIEGFMHEFITPIVKCAKGRMKKVFYTMPEYEAWRLVTNNGQGWRIKYYKGLGTSTTEEAKEYFSDLPTHQIDFSYKGEE-DGDSIDMAFSKKRVEERKDWLRGYTPGTHVDYNVDDMPYSEFVNKELILFSRADNMRSIPCLVDGFKPSQRKVLYCCFKRNLKVEIKVAQLAGYVSEHSSYHHGEASLHGTIINMAQDFTGSNNTNLLSPNGQFGTRILGGKDAASPRYIFTKLESIAYKIFDPNDDSLLNFLDEDGQLIEPEWYVPVIPMVLVNGSEGIGTGWSSSIPNYNPLDLIENLRARIKGEEATRPLVPWYRGFTGSIEERSNARDGDSQAFIVRGIYHVTGDSTLVITELPVKVWTQTYKQFLETLLEN------------------------------ETIKDFKENHTDSKVLFTISMTPEKLQEIVQSPGGIVKKFKLETSISASNMHLFDADGRIQKYDSPHAIINAFYGIRLAYYGKRKAAMLNKLTDQIQMLANKVKFILAVISGDLIVSNRKKVELLQELADSGYDTIHPKSSKVKTTNEEQEKDSEESDDE---DGTAELAKGYNYLLSMAIWSLTKERVEKLRAELKEREVEYKSLAAKSIEELWLTDLEQLEQLLHQTEEIRQK 1211          

BLAST of mRNA_E-siliculosus-1a_M_contig98.17730.1 vs. uniprot
Match: W4H243_9STRA (DNA topoisomerase 2 n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=W4H243_9STRA)

HSP 1 Score: 1326 bits (3432), Expect = 0.000e+0
Identity = 696/1195 (58.24%), Postives = 860/1195 (71.97%), Query Frame = 0

Query:   37 GGGQTVEQRYQKKTQLEHILLRPDTYIGSTEKMSQEMWVYDEVADSIVKRAVTFVPGLYKIFDEIIVNAADNKQRDENMSKLKVTVDAESGKISVWNDGAGIPIQMHKEHHIYVPELIFGNLLTGSNFDDDQKKTTGGRNGFGAKLANIYSTEFVVETADRDAGKHYRQFFRNNMQEKEAPEIDCDYEGPDFTCITFTPDWKRFHMTGMEADTIALLRKRVYDMAGCMGGYAGANVKVYLDNRRVKVSGFKDYMSMYEGMETPVIFESIGDRWEVGVAASADSVFQQVSYVNAICTVKGGTHVECVANQVINKLLPVLKRKHKAE-VKGHFVKNHLCIFVNGLVENPAFNSQSKEALTSKDTLFGSVPKISDAGLKKLEKSEIVDRVLSWAKHKQNQQLNKKSGKKSSKLTGITKLDDANHAGTAKSQDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLKGKLLNVREATGPQVLKNEEIQNVVKILGLKFNHKYDDTKQLRYGHLMIMTDQDHDGSHIKGLLINFVHHYWPSLLKIDGFLQEFITPIVKVSKGRNELSFYTVPEYTVWKEGHANGKGWNAKYYKGLGTSTAKEAKEYFAALDTHQLEFEWQDDDRDDDLIDMAFSKKRVEDRKEWLRGFKAGTHADYNVESVTYESFINKELILFSMEDNKRSIPCLVDGFKPSQRKVLFSCFKRNLKKEIKVVQLAGYVSEHSAYHHGETSLNGTITAMAQNFTGSNNLNLLTPGGQFGTRLMGGKDAASPRYIFTALQKAARAVFHPDDDALLTYLDDDGLSIEPEFYMPIVPLILVNGSEGIGTGWSSNVPNYSPHDVIENLKRRIKGDQFVEMQPWYKGFAGSIEAKVGKKGFEGSYTVKGIVNRVDDTTVEITELPVRKWTQDYKQFLESLLPGAEDAKSKDAKGNXXXXXXXXXXXXXXKSPTRCIKDFKENHTDTTVHFTVTMSMEDLDALEKGDG-LYKFFKLESSVSTNNMVLFDQDGHLERYQSPQHIMEEFITLRSEFYGRRKTSLLNKLQSEWSKLDNKVRFILAVVEDRMQVNNRKKSELLEQLKREGYDLFLPATSKLRVAGGAEDGEGEDGHEESAADDSDTLSKGYDYLLGMKIWSLTLERVKLLENELEAKAEEMRILKATAPSELWLKDLDALGDVLDEIDEIIRKQEVEDAKQRKNAGKRKGG 1229
            GGG TVEQ YQKKTQLEHILLRPDTY+GS E   Q +WVYD+    +V++ VT  PGLYKIFDEIIVNA DNKQRD  M  LKVT+D+E G+ISVWN+G GIP+ MHKEH++YVPELIFG+LLTGSNFDD +KKTTGGRNG+GAKLANI+S EFVVETA R+ GK YRQ F +NM  K AP+I   +   DFTCITFTPD+KRF MTG++ D +AL +KRVYD+AG        ++ VYL+  ++ V  F  Y+++Y   +  VIF+   +RW+VG+  S D  FQQVS+VN ICT KGG HV  +A+Q+  KL+ V+K+++K E VK  ++KNHLC++V+ L++NPAF+SQ+KE LT++   FGS   +S+  +K +EKS +V+ +LS+AK KQ  +L K SG KS KLTGI+KLDDAN AG+AK +DCTLILTEGDSAK LA+SGL VVGRD YGVFPLKGKLLNVREA+   ++KNEEIQN+VKILGLK+   YD TK LRYGHLMIM DQDHDGSHIKGL+INF+HH+WPSL+ +D F+QEFITPIVK +KG     FYT+PEY  W+    N KGW  KYYKGLGTS  +EA+EYF+ L+THQ+ F + + + D D IDMAFSKKRVEDRKEWLR F  GT  DY V+ + Y  F+NKELILFSM DN RSIP +VDGFKPSQRKVLFSCFKRNLKKEIKV QL+GY+SEHSAYHHGE +L+GTI  MAQNF GSNN+NLL P GQFGTRLMGGKDAAS RY+FT L+   R VF+P DDA+L YL+D+G SIEP++YMPI+P+ LVNGS+GIGTGWS  +PNY+P DVIENL+RRI+ +    M P+Y+GF G I  K G   F     V+G    +DD+T+ I+ELPV+ WTQ YKQ+LE+LL                                  IKDFKENHTDTTV FTV +S E L A++   G + K FKLESS++ +NM LFD +G ++ Y SP  I+EEF  LR E YG RK ++L +L+ +   L NK+RFILAV+E  + VNNRKK ELLE+L+++ YD      +   VA   E  E          D+    +K YDYLL M +WSLT ERV+ L N+LEAK EE  ++ AT    +WLKDLD L   L + +E    QE    KQ   AGK   G
Sbjct:   23 GGGLTVEQIYQKKTQLEHILLRPDTYVGSIEPAEQTLWVYDDENSKMVQKKVTICPGLYKIFDEIIVNACDNKQRDSTMDTLKVTIDSEKGEISVWNNGNGIPVVMHKEHNVYVPELIFGHLLTGSNFDDKKKKTTGGRNGYGAKLANIFSKEFVVETASREQGKRYRQVFSDNMSVKGAPKI-TSWSKKDFTCITFTPDFKRFQMTGLDDDIVALFKKRVYDIAGV----TDKSLNVYLNEEKIAVKSFSQYVALYGTAD--VIFDKPDERWQVGLGLSDDG-FQQVSFVNGICTTKGGQHVNYLADQITTKLIAVVKKRNKGEAVKPAYIKNHLCLYVSALIDNPAFDSQTKETLTTRPPSFGSTYTLSEKFMKAVEKSGLVENILSFAKLKQTAELKKTSGTKSVKLTGISKLDDANFAGSAKGKDCTLILTEGDSAKALAMSGLAVVGRDYYGVFPLKGKLLNVREASHSVIVKNEEIQNIVKILGLKYGTTYDSTKSLRYGHLMIMADQDHDGSHIKGLVINFIHHFWPSLMGLDNFVQEFITPIVKATKGNRSEVFYTIPEYEAWRGQMNNAKGWYIKYYKGLGTSKPEEAREYFSDLNTHQIGFTY-NGEPDGDAIDMAFSKKRVEDRKEWLRAFVPGTFVDYAVQDMPYTEFVNKELILFSMADNIRSIPSMVDGFKPSQRKVLFSCFKRNLKKEIKVAQLSGYISEHSAYHHGEVNLHGTIIGMAQNFVGSNNINLLAPNGQFGTRLMGGKDAASARYVFTNLEYITRLVFNPLDDAILNYLEDEGQSIEPQWYMPIIPMSLVNGSDGIGTGWSCGIPNYNPLDVIENLRRRIQAEPMNSMVPFYRGFKGDIVQKGGTDNF----LVQGKFEVIDDSTIVISELPVKTWTQSYKQYLETLLEA------------------------------NTIKDFKENHTDTTVLFTVILSPESLAAIQNAPGGIPKKFKLESSLAISNMHLFDSNGQIKHYSSPLDIIEEFYGLRLEHYGFRKAAILKRLEHDILVLSNKMRFILAVIEGELIVNNRKKKELLEELRKQKYDPMPKNVTAPTVAAAGE--EXXXXXXXXXXDNVGVSAKDYDYLLSMALWSLTAERVQNLRNDLEAKNEERDVVFATTLETMWLKDLDELEVGLGKDEENRLAQEHSTKKQ---AGKATSG 1169          

BLAST of mRNA_E-siliculosus-1a_M_contig98.17730.1 vs. uniprot
Match: A0A1V9Z4P5_9STRA (DNA topoisomerase 2 n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9Z4P5_9STRA)

HSP 1 Score: 1317 bits (3408), Expect = 0.000e+0
Identity = 687/1190 (57.73%), Postives = 865/1190 (72.69%), Query Frame = 0

Query:   39 GQTVEQRYQKKTQLEHILLRPDTYIGSTEKMSQEMWVYDEVADSIVKRAVTFVPGLYKIFDEIIVNAADNKQRDENMSKLKVTVDAESGKISVWNDGAGIPIQMHKEHHIYVPELIFGNLLTGSNFDDDQKKTTGGRNGFGAKLANIYSTEFVVETADRDAGKHYRQFFRNNMQEKEAPEIDCDYEGPDFTCITFTPDWKRFHMTGMEADTIALLRKRVYDMAGCMGGYAGANVKVYLDNRRVKVSGFKDYMSMYEGMETPVIFESIGDRWEVGVAASADSVFQQVSYVNAICTVKGGTHVECVANQVINKLLPVLKRKHKAE-VKGHFVKNHLCIFVNGLVENPAFNSQSKEALTSKDTLFGSVPKISDAGLKKLEKSEIVDRVLSWAKHKQNQQLNKKSGK-KSSKLTGITKLDDANHAGTAKSQDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLKGKLLNVREATGPQVLKNEEIQNVVKILGLKFNHKYDDTKQLRYGHLMIMTDQDHDGSHIKGLLINFVHHYWPSLLKIDGFLQEFITPIVKVSKGRNELSFYTVPEYTVWKEGHANGKGWNAKYYKGLGTSTAKEAKEYFAALDTHQLEFEWQDDDRDDDLIDMAFSKKRVEDRKEWLRGFKAGTHADYNVESVTYESFINKELILFSMEDNKRSIPCLVDGFKPSQRKVLFSCFKRNLKKEIKVVQLAGYVSEHSAYHHGETSLNGTITAMAQNFTGSNNLNLLTPGGQFGTRLMGGKDAASPRYIFTALQKAARAVFHPDDDALLTYLDDDGLSIEPEFYMPIVPLILVNGSEGIGTGWSSNVPNYSPHDVIENLKRRIKGDQFVEMQPWYKGFAGSIEAKVGKKGFEGSYTVKGIVNRVDDTTVEITELPVRKWTQDYKQFLESLLPGAEDAKSKDAKGNXXXXXXXXXXXXXXKSPTRCIKDFKENHTDTTVHFTVTMSMEDLDALEKG-DGLYKFFKLESSVSTNNMVLFDQDGHLERYQSPQHIMEEFITLRSEFYGRRKTSLLNKLQSEWSKLDNKVRFILAVVEDRMQVNNRKKSELLEQLKREGYDLFLPATSKLRVAGGAEDGEGEDGHEESAADDSDTLSKG-YDYLLGMKIWSLTLERVKLLENELEAKAEEMRILKATAPSELWLKDLDALGDVLDEIDEIIRKQEVEDAKQRKNAG 1224
            G TVEQ YQKKTQLEHILLRPDTY+GS E ++Q MWV+D     +V + +T  PGLYKIFDEIIVNA DNKQRD++M  LKVT D E+G ISVWN+G GIP+ +HKEH++YVPELIFG+LLTGSNFDD++KKTTGGRNG+GAKLANI+STEF+VETA ++ GK YRQ F NNM EK  P+I   +   DFTCITFTPD+ RF MTG++ D I+L +KRVYD+AG         +KVYL+  +++V  F  Y+ MY    T VIF+   DRW+VGV  S D  FQQVS+VN ICT KGG HV  +A+Q+  KL   +K+K+K E VK   +KNHLCI+V+ L++NPAF+SQ+KE LT++   FGS   +SD  LK++EK+ +V+R+LS+AK KQ  +L + +G  K  +LTGITKLDDAN AG AK   CTLILTEGDSAK LA+SGL VVGRD YGVFPL+GKLLNVREA+   +LKNEEI +++ ILGL    +Y+ TK LRYG +MIM DQDHDGSHIKGL+INF+H+YWPSL+ I+ F+QEFITPI+K +KG   LSFYT+PEY  W+    NG+GW+ KYYKGLGTSTAKEAKEYF+ L+ HQ+ F+++    DDD I+MAFSKKR+EDRKEWLR F  GTH DY+V+++ Y  F+NKELILFSM DN RSIP +VDGFKPSQRKVLFSCFKRNLKKEIKV QLAGYVSEHSAYHHGE SL+GTI  MAQ+F GSNNL+LL+P GQFGTRL GGKDAAS RY+FT L+   R VFHP DD++L+YL+++GLSIEPE+YMP++PL LVNGS+GIGTGWSS++PNY+P D+I+N+++RIK +    MQPWY+GF GSI     +KG   ++ V+G+   VD++T+ I+ELPV+ WTQ YK+ LE +L                               T  IKDF+ENHTDTTV FT+TM    L A++    G+ K FKLE+S+ST+NM LFD  G ++ Y+SP  I++EF TLR ++YGRRK ++L +L +E   L NK+RFILAV+E  + VNNRKK +LLE+L+R  YD       K +VA             +   DD   +S G YDYLL M +WSLT ERV+ L N+L +K  E   + AT   E+WL DLDAL   L   +     QE    KQ++  G
Sbjct:   35 GLTVEQIYQKKTQLEHILLRPDTYVGSIEPVTQNMWVFDSEEKKMVNKKITVCPGLYKIFDEIIVNACDNKQRDKSMDTLKVTFDQETGAISVWNNGRGIPVVLHKEHNVYVPELIFGHLLTGSNFDDNKKKTTGGRNGYGAKLANIFSTEFIVETASKEDGKRYRQVFTNNMGEKGEPKITA-WSKKDFTCITFTPDFARFQMTGLDDDIISLFKKRVYDIAGV----TDKTLKVYLNEEQIEVKTFPQYIEMYN--PTEVIFDKSDDRWQVGVGVSDDG-FQQVSFVNGICTTKGGQHVNYIADQIATKLSATIKKKNKGETVKPALIKNHLCIYVSALIDNPAFDSQTKETLTTRANAFGSTFTLSDKFLKQVEKTSLVERILSFAKFKQTAELQRAAGGGKKKRLTGITKLDDANFAGGAKGHLCTLILTEGDSAKALAMSGLSVVGRDYYGVFPLRGKLLNVREASHSAILKNEEISSIINILGLDLKKQYESTKNLRYGRVMIMADQDHDGSHIKGLVINFIHNYWPSLMGIENFVQEFITPIIKATKGGRVLSFYTMPEYENWRNSTNNGRGWDIKYYKGLGTSTAKEAKEYFSDLNKHQIAFKYEGP-VDDDAIEMAFSKKRIEDRKEWLRAFVPGTHIDYDVQNMGYADFVNKELILFSMADNIRSIPSVVDGFKPSQRKVLFSCFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLHGTIINMAQDFVGSNNLHLLSPNGQFGTRLKGGKDAASARYVFTKLETLTRMVFHPLDDSVLSYLEEEGLSIEPEWYMPVIPLALVNGSDGIGTGWSSSIPNYNPVDIIKNIRQRIKAEPMEAMQPWYRGFKGSIV----QKGSGDNFLVQGLFEVVDESTLVISELPVKTWTQSYKKILEKMLE------------------------------TNKIKDFRENHTDTTVLFTITMEPAALAAVQNEVGGILKEFKLETSISTSNMHLFDAAGQIKHYRSPLEIIDEFYTLRIDYYGRRKVAILKRLDTEIMALSNKMRFILAVIEGSLVVNNRKKKDLLEELRRLKYDPM----PKKKVAKXXXXXXXXXXXADGTTDD---VSAGDYDYLLSMALWSLTAERVENLRNDLHSKEAERDKIFATPLEEMWLADLDALEAGLAGHETARLAQEAAQLKQQRKQG 1174          

BLAST of mRNA_E-siliculosus-1a_M_contig98.17730.1 vs. uniprot
Match: A0A024UPN1_9STRA (DNA topoisomerase 2 n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024UPN1_9STRA)

HSP 1 Score: 1316 bits (3405), Expect = 0.000e+0
Identity = 686/1201 (57.12%), Postives = 866/1201 (72.11%), Query Frame = 0

Query:   36 NGGGQTVEQRYQKKTQLEHILLRPDTYIGSTEKMSQEMWVYDEVADSIVKRAVTFVPGLYKIFDEIIVNAADNKQRDENMSKLKVTVDAESGKISVWNDGAGIPIQMHKEHHIYVPELIFGNLLTGSNFDDDQKKTTGGRNGFGAKLANIYSTEFVVETADRDAGKHYRQFFRNNMQEKEAPEIDCDYEGPDFTCITFTPDWKRFHMTGMEADTIALLRKRVYDMAGCMGGYAGANVKVYLDNRRVKVSGFKDYMSMYEGMETP-VIFESIGDRWEVGVAASADSVFQQVSYVNAICTVKGGTHVECVANQVINKLLPVLKRKHKAE-VKGHFVKNHLCIFVNGLVENPAFNSQSKEALTSKDTLFGSVPKISDAGLKKLEKSEIVDRVLSWAKHKQNQQLNKKSGKKSSKLTGITKLDDANHAGTAKSQDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLKGKLLNVREATGPQVLKNEEIQNVVKILGLKFNHKYDDTKQLRYGHLMIMTDQDHDGSHIKGLLINFVHHYWPSLLKIDGFLQEFITPIVKVSKGRNELSFYTVPEYTVWKEGHANGKGWNAKYYKGLGTSTAKEAKEYFAALDTHQLEFEWQDDDRDDDLIDMAFSKKRVEDRKEWLRGFKAGTHADYNVESVTYESFINKELILFSMEDNKRSIPCLVDGFKPSQRKVLFSCFKRNLKKEIKVVQLAGYVSEHSAYHHGETSLNGTITAMAQNFTGSNNLNLLTPGGQFGTRLMGGKDAASPRYIFTALQKAARAVFHPDDDALLTYLDDDGLSIEPEFYMPIVPLILVNGSEGIGTGWSSNVPNYSPHDVIENLKRRIKGDQFVEMQPWYKGFAGSIEAKVGKKGFEGSYTVKGIVNRVDDTTVEITELPVRKWTQDYKQFLESLLPGAEDAKSKDAKGNXXXXXXXXXXXXXXKSPTRCIKDFKENHTDTTVHFTVTMSMEDLDALEKGDG-LYKFFKLESSVSTNNMVLFDQDGHLERYQSPQHIMEEFITLRSEFYGRRKTSLLNKLQSEWSKLDNKVRFILAVVEDRMQVNNRKKSELLEQLKREGYDLFLPATSKLRVAGGAEDG----EGEDGHEESAADDSDTLSKGYDYLLGMKIWSLTLERVKLLENELEAKAEEMRILKATAPSELWLKDLDALGDVLDEIDEIIRKQEVEDAKQRKNAGKRKGG 1229
            NGGG T+EQ YQKKTQLEHILLRPDTY+GS E   Q +WV+D+    +V++ VT  PGLYKIFDEIIVNA DNKQRD  M  LKVT+DAE G+ISVWN+G GIP+ MH+EH++YVPELIFG+LLTGSNFDD +KKTTGGRNG+GAKLANI+S EF+VETA R+ GK YRQ F NNM  K AP+I   +   DFTCITFTPD++RF MTG++ D +AL +KRVYD+AG        ++ VYL+  ++ V  F  Y+++Y    TP VI+    +RW+VG+  S D  FQQVS+VN ICT KGG HV  +A+Q+ NKL+ ++K+++K E VK  ++KNHLC++V+ L++NPAF+SQ+KE LT++   FGS   +SD  +K +EKS +V+ +LS+AK KQ   L K SG KS KLTGI+KLDDAN AG+AK +DCTLILTEGDSAK LA+SGL VVGRD YGVFPLKGKLLNVREA+   ++KNEEIQN+VKILGLK+   Y+ TK LRYGHLMIM DQDHDGSHIKGL+INF+HH+WPSL+ +D F+QEFITPIVK +KG     FYT+PEY  W+    + KGW  KYYKGLGTS  ++A+EYF+ L+THQ+ F + + + D D IDMAFSKKRVEDRK+WLR F  GT+ DY V+ + Y  F+NKELILFSM DN RSIP +VDGFKPSQRKVLFSCFKRNLKKEIKV QLAGY+SEHSAYHHGE +L+GTI  MAQNF GSNN+NLL+P GQFGTRLMGGKDAAS RY+FT ++   R VFHP DD +L YL+D+G SIEP++YMP +P+ LVNGS+GIGTGWS  +PNY+P D+IENL+RRI+ +    M P+Y+GF G I  K G   F     V+G    VD++T+ I+ELPV+ WTQ YKQFLE+LL    DA +                          IKDFKENHTDT V FTV +S E L A+++  G + K FKLESS++ +NM LFD +G ++ Y SP  I+E+F  LR E+YG RK ++L +L+ +   L NK+RFILAV+E  + VNNRKK +LLE+L+ + +D     ++   VA   ED     E +D H       S+  +K YDYLL + +WSLT ERV+ L ++LE K +E   + +T    +WLKDL  L   L + +     QE    KQ   AGK K G
Sbjct:   12 NGGGLTIEQIYQKKTQLEHILLRPDTYVGSIEPAEQSLWVFDDENSKMVQKKVTICPGLYKIFDEIIVNACDNKQRDSTMDTLKVTIDAEKGEISVWNNGNGIPVVMHQEHNVYVPELIFGHLLTGSNFDDKKKKTTGGRNGYGAKLANIFSKEFIVETASREQGKRYRQVFSNNMSVKGAPKI-TSWSKKDFTCITFTPDFERFQMTGLDDDIVALFKKRVYDIAGI----TDKSLNVYLNEEKIAVKSFPQYVALYG---TPDVIYGQPDERWQVGLGLSDDG-FQQVSFVNGICTTKGGQHVNYIADQITNKLVGIVKKRNKGEAVKPAYIKNHLCLYVSALIDNPAFDSQTKETLTTRSPAFGSTYTLSDKFMKAVEKSGLVENILSFAKLKQTAALKKTSGTKSVKLTGISKLDDANFAGSAKGKDCTLILTEGDSAKALAMSGLAVVGRDYYGVFPLKGKLLNVREASHSVIVKNEEIQNIVKILGLKYGTTYESTKSLRYGHLMIMADQDHDGSHIKGLVINFIHHFWPSLMGLDNFVQEFITPIVKATKGNRSEVFYTIPEYEAWRNRMVDTKGWYIKYYKGLGTSKPEDAREYFSDLNTHQIGFTY-NGEADGDAIDMAFSKKRVEDRKDWLRAFVPGTYVDYAVQDMPYTEFVNKELILFSMADNIRSIPSMVDGFKPSQRKVLFSCFKRNLKKEIKVAQLAGYISEHSAYHHGEVNLHGTIIGMAQNFVGSNNINLLSPNGQFGTRLMGGKDAASARYVFTNVESVTRLVFHPLDDPILNYLEDEGQSIEPQWYMPTIPMSLVNGSDGIGTGWSCGIPNYNPLDIIENLRRRIQAEPMKSMVPFYRGFKGEIVQKGGSDNF----LVQGRYEIVDESTIVISELPVKTWTQSYKQFLETLL----DANT--------------------------IKDFKENHTDTAVLFTVVLSPESLAAIQQAPGGIMKKFKLESSLAISNMHLFDSNGQIKHYSSPLDIIEDFYGLRLEYYGYRKAAILKRLEHDIMILSNKMRFILAVIEGELVVNNRKKQDLLEELRMKKFDPMPRKSTAPTVAAAGEDDPVAEEDDDSH-------SEVTAKDYDYLLSLPMWSLTAERVQSLRDDLEEKNQERDTVFSTTLESMWLKDLHELELGLSKEEMKRLAQEESTMKQ---AGKAKSG 1158          

BLAST of mRNA_E-siliculosus-1a_M_contig98.17730.1 vs. uniprot
Match: A0A421GVE2_9STRA (DNA topoisomerase 2 n=4 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A421GVE2_9STRA)

HSP 1 Score: 1315 bits (3404), Expect = 0.000e+0
Identity = 685/1239 (55.29%), Postives = 871/1239 (70.30%), Query Frame = 0

Query:   40 QTVEQRYQKKTQLEHILLRPDTYIGSTEKMSQEMWVYDEVADSIVKRAVTFVPGLYKIFDEIIVNAADNKQRDENMSKLKVTVDAESGKISVWNDGAGIPIQMHKEHHIYVPELIFGNLLTGSNFDDDQKKTTGGRNGFGAKLANIYSTEFVVETADRDAGKHYRQFFRNNMQEKEAPEIDCDYEGPDFTCITFTPDWKRFHMTGMEADTIALLRKRVYDMAGCMGGYAGANVKVYLDNRRVKVSGFKDYMSMYEGMETP-----------------------------------------------VIFESIGDRWEVGVAASADSVFQQVSYVNAICTVKGGTHVECVANQVINKLLPVLKRKHKAE-VKGHFVKNHLCIFVNGLVENPAFNSQSKEALTSKDTLFGSVPKISDAGLKKLEKSEIVDRVLSWAKHKQNQQLNKKS--GKKSSKLTGITKLDDANHAGTAKSQDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLKGKLLNVREATGPQVLKNEEIQNVVKILGLKFNHKYDDTKQLRYGHLMIMTDQDHDGSHIKGLLINFVHHYWPSLLKIDGFLQEFITPIVKVSKGRNELSFYTVPEYTVWKEGHANGKGWNAKYYKGLGTSTAKEAKEYFAALDTHQLEFEWQDDDRDDDLIDMAFSKKRVEDRKEWLRGFKAGTHADYNVESVTYESFINKELILFSMEDNKRSIPCLVDGFKPSQRKVLFSCFKRNLKKEIKVVQLAGYVSEHSAYHHGETSLNGTITAMAQNFTGSNNLNLLTPGGQFGTRLMGGKDAASPRYIFTALQKAARAVFHPDDDALLTYLDDDGLSIEPEFYMPIVPLILVNGSEGIGTGWSSNVPNYSPHDVIENLKRRIKGDQFVEMQPWYKGFAGSIEAKVGKKGFEGS-YTVKGIVNRVDDTTVEITELPVRKWTQDYKQFLESLLPGAEDAKSKDAKGNXXXXXXXXXXXXXXKSPTRCIKDFKENHTDTTVHFTVTMSMEDLDALEKGDG-LYKFFKLESSVSTNNMVLFDQDGHLERYQSPQHIMEEFITLRSEFYGRRKTSLLNKLQSEWSKLDNKVRFILAVVEDRMQVNNRKKSELLEQLKREGYDLFLPATSKLRVAGGAEDGEGEDGHEESAADDSDTLSKGYDYLLGMKIWSLTLERVKLLENELEAKAEEMRILKATAPSELWLKDLDALGDVLDEIDEIIRKQEVEDAKQRKNAGKR 1226
            Q++EQ YQKKTQLEHILLRPDTY+GS E + Q MWV+DE A  +V + + FVPGLYKIFDEIIVNA DNKQRD++M  LKV +D E  +ISVWN+G GIPI +HKEH +YVPELIFG+LLTGSNFDD +KKTTGGRNG+GAKL NI+S EF VETAD +  + Y+Q F NNM +K  P I   +   DFTCITF PD +RF M  ++   ++L +KRVYD+AG     +G  + VYL+  ++ +  F  Y++MY G  +                                                VIFE   +RW+VGV  S D  FQQVS+VN ICT KGG HV  +A+Q+ +KL+  LK+K+K E VK +++KNH+ ++V+ L++NPAF+SQ+KE LT++   FGS   +SD  LK++EKS +V+R+LS+A  KQ  +L +K   G K S+LTGI KLDDANHAGTAK +DCTLILTEGDSAKT+AV GL VVGRD YGVFPL+GK+LNVREA+  Q+LKNEEIQNVVKILGLK+   Y+ TK LRYGHLMIM DQDHDGSHIKGL+IN +HH+WPSLL +DGFLQEFITPIVK +KGR    FYT+PEY VW+E    G+GW  KYYKGLGTSTA E KEYF+ L THQ+ F ++ D  D D+IDMAFSKKRVEDRK WLRG++ GT+ DY+V+ + Y  F+NKELILFSM DN RSIP +VDGFKPSQRKVLF CFKRNL+ E+KV QLAGYVSEHSAYHHGE  L G I  MAQ+F GSNN++LL+P GQFGTRLMGGKDAAS RYIFT L++  R ++ P D+ +L YLD+DG SIEPE+Y+PI+P++LVNGS+GIGTGWSS+VPNY+P D+I+ L+R + G++   + PWY+GF G I  K   +G + S Y V+G+   +DD+T+ I+ELPV+ WTQ YKQFLE LL    DA +                          IKDFKENHTD  V F +T+  + L  + K  G + K FKLESS+ST+NM LFD  GH+++Y+SP+ +MEEF  LR E+Y RRK ++L KLQ +   L NK RF+L+VVE ++ VNNRK+ +LLE+L  EGYD  LP  SK +     E  E +  +E++AAD      +GYDYLL MKIWSLT ERV  L  EL+ + +E  +L++    +LW  +LDAL  +L E  E  R++ +    ++KN GK+
Sbjct:   79 QSIEQIYQKKTQLEHILLRPDTYVGSIEHVEQSMWVFDEAAKKMVMKKINFVPGLYKIFDEIIVNACDNKQRDKSMDTLKVDIDVEKAEISVWNNGKGIPIVLHKEHKVYVPELIFGHLLTGSNFDDKKKKTTGGRNGYGAKLTNIFSNEFTVETADSNTKQRYKQVFENNMSKKNPPSIS-KWARKDFTCITFKPDLERFQMPILDDGIVSLFKKRVYDVAGV----SGKRLNVYLNGEKIAIKSFPQYVTMYPGPRSSESEKETKXXXXANPVXXXXXXXXXXXXXXXXXXXXXXXXPSNITNNGVIFEKANERWQVGVGLSDDG-FQQVSFVNGICTTKGGQHVNYIADQITSKLVATLKKKNKGEAVKPNYIKNHMIVYVSALIDNPAFDSQTKETLTTRPAHFGSTITLSDKFLKQVEKSGMVERILSFAAFKQTAELRRKGSGGGKKSRLTGIPKLDDANHAGTAKGKDCTLILTEGDSAKTVAVGGLAVVGRDYYGVFPLRGKMLNVREASHSQILKNEEIQNVVKILGLKYGQMYESTKGLRYGHLMIMADQDHDGSHIKGLVINLIHHFWPSLLLVDGFLQEFITPIVKCTKGRTLEIFYTMPEYNVWRERTNQGRGWTIKYYKGLGTSTAAETKEYFSDLQTHQIGFTYEGDG-DADVIDMAFSKKRVEDRKNWLRGYEPGTYVDYDVDEMGYTEFVNKELILFSMADNIRSIPSIVDGFKPSQRKVLFCCFKRNLRTELKVAQLAGYVSEHSAYHHGEAGLQGCIVNMAQDFVGSNNVHLLSPIGQFGTRLMGGKDAASSRYIFTNLEQLTRRIYDPLDNDVLNYLDEDGQSIEPEWYIPIIPMVLVNGSDGIGTGWSSSVPNYNPLDIIKGLRRMLNGEEVTPLAPWYRGFTGHIIEKTNSRGTDSSNYLVQGLFEVIDDSTLVISELPVKSWTQTYKQFLEGLL----DAGT--------------------------IKDFKENHTDVKVLFKITLDPKTLSDIAKAPGGIVKKFKLESSLSTSNMHLFDAAGHIKKYESPREVMEEFYGLRLEYYDRRKRAMLQKLQDQIKLLSNKARFVLSVVEGKLTVNNRKRQDLLEELIAEGYDQILPKASKKKSDSNTEANESD--NEDNAAD----AGRGYDYLLSMKIWSLTKERVDKLRAELQDREQEYNVLESRTIQDLWTTNLDALEQMLVET-EAERERVLAHTPKKKNRGKK 1273