prot_E-siliculosus-1a_M_contig91.17214.1 (polypeptide) Ectocarpus siliculosus Ec864m_EcPH12_78m male

BLAST of mRNA_E-siliculosus-1a_M_contig91.17214.1 vs. uniprot
Match: D7FTW8_ECTSI (Calpain catalytic domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FTW8_ECTSI)

HSP 1 Score: 1006 bits (2602), Expect = 0.000e+0
Identity = 506/584 (86.64%), Postives = 516/584 (88.36%), Query Frame = 0

Query:    1 MEELFAAATGCLNPLVAASYLERAGYDLTRAVNHFLDSPKNDTAASRGHSDYCRSASCGDDNSGPRSFKRQRAAMAGEKQQQQLLKFEHARPSLSDTSCSSVTASVGMPSSSGMAEEIHAKPCDSILGAPVPKTTTAPSAGWEAIAERCQRQGAQYEDGDFLPVPSSLDGRIGRGCSASSRGCTGGGGTETAEGHARCKCGTIARVITVNKAGVNHGRLFYGCITRKCDFFRWADDAPHTRQALSLVWRRFSPPRFKLSSAAPRGAEGVGGSFKPEHVRQGSVGDCWFLAGLAVVSERADLIGRVVGSNGPLADELGCFEVNLFKDGRWESVVVDSWLPCKDPARLKGKEKGLDHLPAFAKAFNNQIWPCIVEKAFAKQHGSYDAISGGHVCDAFEALTGAPTETVVLDNDDSEGNWAQLLSFNQAGFPMGCATAWDPTRGLRDVGLVSTHAYSVLEVRELPPGVRGARQPTIRDWAGLGSAAAAAEQGRRRDGEASGQPLRLVRVRNPWGKREWIGDWGEKSEKWSDSVRAELGWSEKNDGTFWMTWQDFMTRFQLVDVCKARRDWSHASVTNHSIPSPKTCR 584
            MEELFAAATGCLNPLVAASYLERAGYDLTRAVNHFLDSPKNDTAASR HSDYC+SASCGDDNS PRS KRQRAAMAGEKQQQQLLKFEHARP LSDTS SSVTASVGMP SSGMAEEIHAKPCDSI GAPVPK+TTAPSAGWEAIAERCQRQGAQYEDGDFLPVPSSLDGRIGRGCSASSR CT GGGTETAEGHARCKCGT ARVITVNKAGVN GRLFYGCITRKCDFFRWADDAPHTRQALSLVWRRFSPPRFKLSSAAPRGAEGVGGSFKPEHV QG+VGDCWFLAGLAVVSERADLIGRVVGSNGPLADELGCFEVNLFKDGRWESVVVDSWLPCKDPARLKGKEKGLDHLPAFAKAFNNQIWPCIVEKAFAKQHGSYDAISGGHVCDAFEALTGAPTETV+LDNDDSEGNWAQLLSFNQAGFPMGCATAWDPTR                     PP      +P  R +      AA   Q  R  GE + +   L  +    GKREWIGDWGEKSEKWSDSVRAELGWSEKNDGTFWMTWQDFMTRFQLVDVCKARRDWSHASVT H+IPSPK CR
Sbjct:    1 MEELFAAATGCLNPLVAASYLERAGYDLTRAVNHFLDSPKNDTAASRSHSDYCKSASCGDDNSDPRSLKRQRAAMAGEKQQQQLLKFEHARPLLSDTSSSSVTASVGMPFSSGMAEEIHAKPCDSIRGAPVPKSTTAPSAGWEAIAERCQRQGAQYEDGDFLPVPSSLDGRIGRGCSASSRDCTDGGGTETAEGHARCKCGTSARVITVNKAGVNQGRLFYGCITRKCDFFRWADDAPHTRQALSLVWRRFSPPRFKLSSAAPRGAEGVGGSFKPEHVMQGAVGDCWFLAGLAVVSERADLIGRVVGSNGPLADELGCFEVNLFKDGRWESVVVDSWLPCKDPARLKGKEKGLDHLPAFAKAFNNQIWPCIVEKAFAKQHGSYDAISGGHVCDAFEALTGAPTETVMLDNDDSEGNWAQLLSFNQAGFPMGCATAWDPTR-----------------CENSPPEFE---EPGSRPFETGPGWAAPPRQPSRGGGETAKRLDNLFGL----GKREWIGDWGEKSEKWSDSVRAELGWSEKNDGTFWMTWQDFMTRFQLVDVCKARRDWSHASVTTHNIPSPKACR 560          

BLAST of mRNA_E-siliculosus-1a_M_contig91.17214.1 vs. uniprot
Match: A0A6H5JHG4_9PHAE (Calpain catalytic domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JHG4_9PHAE)

HSP 1 Score: 820 bits (2118), Expect = 6.330e-288
Identity = 454/555 (81.80%), Postives = 470/555 (84.68%), Query Frame = 0

Query:  452 AYSVLEVRELPPGVRGARQPTIRDWAGLGSAAAAAEQGRRRDGEASGQPLRLVRVRNPWGKREWIGDWGEKSEKWSDSVRAELGWSEKNDGTFWMTWQDFMTRFQLVDVCKARRDWSHASVTNHSIPSPKTCRSFRVRPPPPPMAANTEGQTSSQTTGKNPAATAXXXXXXXXXXPSPTPTTWAYITVVQPTKRGRKDGYWYKALGLLVTEMNPSLSARGDTGHSDGVLVASSLGGAKRSLSCEVFLESGKAYTASVVCLGGELGDKISSGRPKSSSAASTLLAESTGFWGFRVTVYSARTVQQIVPGEQRPASPGYFRAGSSLHQALSRRSSEEILAEGVVGRHAVSVRNPLQSFALGGGAALSVVPMEGSAVFFLLVNASSSEVVLRLTLTSLEGATVSTPDSQASPDGEGMTRSTMCPARSQKIALVLARTADHGSFSHEFYYRVLRPAPTAPATKATATASRQGDAGAKAAGKTSSSIGTGAFAEATAATPSFGGGLFSAQALLPGAEELVRRAGGCMYSFSGEGGATEGRGGSTVTGEDGGVGSAGVVRR 1006
            A++  +VRELPPGVRGARQP+IRDWAGLGSAAAAAEQGRRRDGEA GQPLRLVRVRNPWGKREWIGDWGEKSEKWSD VRAELGWSE+NDGTFWMTWQDFM+RFQLVDVCKARRDWSHASVT HSIPSPKTCRSFRVRP PPPMAANTEGQTSSQTTGK  AATA           + TPTTWAYITVVQPTKRGRKDG                                        SLSCEVFLESGKAYTASVVCLGGE  DKI SGRPK  SAASTLLAESTGF GFRVTVYSAR +Q IVPGEQRPASPGYFRA SS+HQALSRRSSEEILA+G+VGR AVSVRNPLQSFALGGGAALSVVPMEGSA+FFLL+N SSSEVVLRLTLTSLEGATVSTPDSQASPDG+GM  STMCPARSQKIALVLARTADHGSFSHEFYYRVLRPAPTAPAT A ATA+RQGDAGAKAA KT SSI T AFAEATAATP FGGGLFSAQALLPGAEELVRRAGG  Y+FSGEGGA EGRGGSTVTGEDGGVGSA V RR
Sbjct:   64 AFTSTKVRELPPGVRGARQPSIRDWAGLGSAAAAAEQGRRRDGEAPGQPLRLVRVRNPWGKREWIGDWGEKSEKWSDRVRAELGWSEQNDGTFWMTWQDFMSRFQLVDVCKARRDWSHASVTTHSIPSPKTCRSFRVRPLPPPMAANTEGQTSSQTTGKKLAATAATTSPTV----AATPTTWAYITVVQPTKRGRKDG----------------------------------------SLSCEVFLESGKAYTASVVCLGGEDSDKILSGRPKF-SAASTLLAESTGFRGFRVTVYSARPLQ-IVPGEQRPASPGYFRAESSIHQALSRRSSEEILAKGIVGRRAVSVRNPLQSFALGGGAALSVVPMEGSALFFLLINVSSSEVVLRLTLTSLEGATVSTPDSQASPDGKGMMSSTMCPARSQKIALVLARTADHGSFSHEFYYRVLRPAPTAPATTAAATATRQGDAGAKAASKTFSSIATRAFAEATAATPFFGGGLFSAQALLPGAEELVRRAGG--YTFSGEGGAVEGRGGSTVTGEDGGVGSAAVARR 570          

BLAST of mRNA_E-siliculosus-1a_M_contig91.17214.1 vs. uniprot
Match: A0A6H5JEW6_9PHAE (GRF-type domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JEW6_9PHAE)

HSP 1 Score: 438 bits (1126), Expect = 6.190e-143
Identity = 230/282 (81.56%), Postives = 235/282 (83.33%), Query Frame = 0

Query:    1 MEELFAAATGCLNPLVAASYLERAGYDLTRAVNHFLDSPKNDTAASRGHSDYC-RSASCGDDNSGPRSFKRQRAAMAGEKQQQQL-LKFEHARPSLSDTSCSSVTASVGMPSSSGMAEEIHAKPCDSILGAPVPKTTTAPSAGWEAIAERCQRQGAQYEDGDFLPVPSSLDGRIGRGCSASSRGCTGGGGTETAEGHARCKCGTIARVITVNKAGVNHGRLFYGCITRKCDFFRWADDAPHTRQALSLVWRRFSPPRFKLSSAAPRGAEGVGGSFKPEHVRQ 280
            MEELFAAATGCL+PLVAASYLERAGYDLTRAVNHFLDSPKN TAASRG SDYC RS                              LKFEHAR  LSDTS SSV ASVGMPSSSGMAEEIHA PCDS+LGAPVPKTTTAPSAGWEA AERCQRQGA+YEDGDFLPVPSSLDGRIGRGCSASSR CTGG GTET EGHARCKCGT ARVITVNKAGVN GRLFYGC+TRKCDFFRWADDAPHTRQALSLVWRRFSPPRFKLSSAAP+G EG GGSFKPEHV Q
Sbjct:    1 MEELFAAATGCLDPLVAASYLERAGYDLTRAVNHFLDSPKNGTAASRGSSDYCCRSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLKFEHARRLLSDTSSSSVPASVGMPSSSGMAEEIHAMPCDSVLGAPVPKTTTAPSAGWEAFAERCQRQGARYEDGDFLPVPSSLDGRIGRGCSASSRDCTGGAGTETTEGHARCKCGTSARVITVNKAGVNQGRLFYGCVTRKCDFFRWADDAPHTRQALSLVWRRFSPPRFKLSSAAPKGEEGGGGSFKPEHVMQ 282          

BLAST of mRNA_E-siliculosus-1a_M_contig91.17214.1 vs. uniprot
Match: A0A5B8MKZ2_9CHLO (Calpain-type cysteine proteinase n=2 Tax=Chloropicon primus TaxID=1764295 RepID=A0A5B8MKZ2_9CHLO)

HSP 1 Score: 352 bits (902), Expect = 1.980e-103
Identity = 210/539 (38.96%), Postives = 291/539 (53.99%), Query Frame = 0

Query:  135 TTAPSAGWEAIAERCQRQGAQYEDGDFLPVPSSLDGR-IGRGCSASSRGCTGGGGTETAEGHARCKCGTIARVITVNKAGVNHGRLFYGCITRKCDFFRWADDA-PHTRQALSLVWRRFSPPRFKLSSAAPRGAEGVGGSFKPEHVRQGSVGDCWFLAGLAVVSERADLIGRVVGSNGPLA--DELGCFEVNLFKDGRWESVVVDSWLPCKDPARLKGKEKGLDHLPAFAKAFN--NQIWPCIVEKAFAKQHGSYDAISGGHVCDAFEALTGAPTETVVLDND--DSEGNWAQLLSFNQAGFPMGCATAWDPTRGLRDVGLVSTHAYSVLEVRELPPGVRGARQPTIRDWAGLGSAAAAAEQGRRRDGEASGQPLRLVRVRNPWGKREWIGDWGEKSEKWSDSVRAELGWSEKNDGTFWMTWQDFMTRFQLVDVCKARRDWSHASVTNH-SIPSPKTCRSFRVRPPPPPMAANTEGQTSSQTTGKNPAATAXXXXXXXXXXPSPTPTTWAYITVVQPTKRGRKDGYWYKALGLLVTEMN 664
            T  PSA W  I   C+ +G  + D DF P   S+DGR + R    +++                C C   A++  V K G N G+ FY C  RKC +FRW+  A  HT QA++L W+RF  P   +  A         G FKP  V QG+VGDCWFL+GLAV++ER DL+ R+     P+   ++ G +E   + DG+WESVVVD+ LP  +  +           PAFAK     +++W  ++EKA+AK H SYDAISGG V +A   LT  PTE +++ ++  +SE  WA+LLSF    FPMGC+T WDPT  L+ VGL   HAYS+LEV EL  GV   +Q  I D+ G G     A++      E  G  LRL+R+RNP G+REW+GDW +KS+KW+ ++ A+LG +EKNDGTFWM++QDF+ RF L++VC A RD+ + +       P+   C  F+V                                            TWAYI V+Q +KRGR D YWY  + + +  ++
Sbjct:  118 TFLPSASWSRILSSCRAEGQSFTDPDFPPAAQSIDGRKVHRNFGKNTK--------------LLCLCKREAKLCKVAKDGKNQGKFFYACELRKCKYFRWSGRALQHTSQAVNLSWKRFDDP-CHIPVAK--------GGFKPSDVLQGAVGDCWFLSGLAVIAERKDLVERIF----PVTQRNDEGVYECKFYIDGQWESVVVDNVLPVDEERK-----------PAFAKLSKKKSELWVPLLEKAYAKVHKSYDAISGGFVSEAMFDLTSYPTEVILVMDEAFESEVTWARLLSFASLKFPMGCSTQWDPT--LQGVGLAPCHAYSILEVIELS-GVTVGQQLKIDDFFGGGKEREKAKK------EEQGSLLRLLRIRNPHGQREWLGDWSDKSDKWTSTLHAKLGRTEKNDGTFWMSYQDFLCRFALIEVCHAHRDFYNVTKRLRLGTPNSWDCAQFKV---------------------------------------DLVEATWAYIMVLQKSKRGRLDNYWYTDVSIAIFRLD 570          

BLAST of mRNA_E-siliculosus-1a_M_contig91.17214.1 vs. uniprot
Match: C1E493_MICCC (Calpain catalytic domain-containing protein n=2 Tax=Micromonas TaxID=38832 RepID=C1E493_MICCC)

HSP 1 Score: 353 bits (906), Expect = 6.200e-102
Identity = 229/605 (37.85%), Postives = 299/605 (49.42%), Query Frame = 0

Query:  135 TTAPSAGWEAIAERCQRQG---AQYEDGDFLPVPSSLDGRIGRGCSASSRGCTGGGGTETAEGHARCKCGTIARVITVNKAGVNHGRLFYGC------------------ITRKCDFFRWADDAPHTRQALSLVWRRFSPPRFKLSSAAPRGAEGVGGSFKPEHVRQGSVGDCWFLAGLAVVSERADLIGRVVG--SNGPLADELGCFEVNLFKDGRWESVVVDSWLPCKDP-------ARLKGKEKGLDHLPAFAKAFNNQIWPCIVEKAFAKQHGSYDAISGGHVCDAFEALTGAPTETVVLDNDD--SEGNWAQLLSFNQAGFPMGCATAWDPTRGLRDVGLVSTHAYSVLEVRELPPGVRGARQPTIRDWAGLGSAAAAAEQGRRRDGEASG--QPLRLVRVRNPWGKREWIGDWGEKSEKWSDSVRAELGWSEKNDGTFWMTWQDFMTRFQLVDVCKARRDWSHASVTNHSIPSPKTCRSFRVRPPPPPMAANTEGQTSSQTTGKNPAATAXXXXXXXXXXPSPTPTTWAYITVVQPTKRGR-KDGYWYKALGLLVTEMNPSLSARGDTGHSDGVLVASSLGGAKRSLSCEVFLESGKAY 704
            T   + GW    E  +       ++ED  F P  SS+DG               G   + +E    C+CG  A++ +V+K G NHGR FY C                   T  C FF+WADDAP +  + ++ WRRF PPRF+L+     GA      F P  VRQG+VGDCW L+ LAVV+ER DL+ RVVG  +  P A   G F + LF  GRW  V VD  LP           A   G     ++  A+++A +NQ+W  +VEKA+AK HGSY AISGG + +    LTG PTET+ LD     SE  W +LLS+  A FPMGCAT  DP+   R+VGLV  HAYSV+EVREL  G    RQ  +  +        A E+    D  A G   PLRL+R+RNPWG++EW G+WG  SE W+  + AELG +  +DGTFWM+W DF+ RF +VDVCKA   W   SV      S +   +F V        A   G +SS                          ++WAY   +QP KRG   + +WY  +G+ V     S   +G T       V   LG  +R    E+  E G  Y
Sbjct:  139 TVDATVGWARTIEALEAAADGRRRFEDASFPPDASSIDG---------------GRADKESESVPCCRCGARAKIKSVHKDGPNHGRWFYACGNQPANAWAARAMAAKRVPTPTCGFFKWADDAPSSESSKAMRWRRFEPPRFRLTRGTASGAP----KFAPSDVRQGAVGDCWLLSALAVVAERQDLVARVVGPAAKCPAATRRGAFLMRLFLAGRWRGVAVDPCLPVSGRSTKVLAGANKVGARSNDEYALAYSRAADNQLWVPLVEKAYAKSHGSYHAISGGWIAEGLLDLTGCPTETIRLDASGFKSEEAWRKLLSYAAAKFPMGCATQGDPSH--REVGLVGNHAYSVMEVRELH-GASVGRQTKLTSFV-------AKERVEDVDAAAGGGGPPLRLLRIRNPWGRKEWSGEWGAGSEVWTSKLGAELGHTRVDDGTFWMSWHDFLCRFTVVDVCKAHEGWHAISV---QAVSAENDPAFEVE------VAPDRGASSS--------------------------SSWAYAMSLQPNKRGLCSERFWYADVGIAVYRKGES-KTQGPTWTP----VTGRLGARERVAQVELMFEVGVTY 674          

BLAST of mRNA_E-siliculosus-1a_M_contig91.17214.1 vs. uniprot
Match: A0A1Y5IBU3_OSTTA (Calpain catalytic domain-containing protein n=2 Tax=Ostreococcus tauri TaxID=70448 RepID=A0A1Y5IBU3_OSTTA)

HSP 1 Score: 328 bits (842), Expect = 1.220e-94
Identity = 216/589 (36.67%), Postives = 301/589 (51.10%), Query Frame = 0

Query:  139 SAGWEAIAER-CQRQGAQ-YEDGDFLPVPSSLDGRIGRGCSASSRGCTGGGGTETAEGHA-RCKCGTIARVITVNKAGVNHGRLFYGCITR--KCDFFRWA--DDAPHTRQALSLVWRRFSPPRFKLSSAAPRGAEGVGGSFKPEHVRQGSVGDCWFLAGLAVVSERADLIGRVVG---SNGPLADELGCFEVNLFKDGRWESVVVDSWLPCKDPARLKGKEKGLDHLPAFAKAFNNQIWPCIVEKAFAKQHGSYDAISGGHVCDAFEALTGAPTETVVLD-ND-DSEGNWAQLLSFNQAGFPMGCATAWDPTRGLRDVGLVSTHAYSVLEVRELPPGVRGARQPTIRDWAGLGSAAAAAEQGRRRDGEASGQPLRLVRVRNPWGKREWIGDWGEKSEKWSDSVRAELGWSEKNDGTFWMTWQDFMTRFQLVDVCKARRDWSHASVTNHSIPSPKTCRSFRVRPPPPPMAANTEGQTSSQTTGKNPAATAXXXXXXXXXXPSPTPTTWAYITVVQPTKRGRKDGYWYKALGLLVTEMNPSLSARGDTGHSDGVLVASSLGGAKRSLSC--EVFLESGKAYTASVVCLGG 713
            S  +EA+A   C+R G + + D  F P P S+DGR                    A G+A +C+CG  A+V  V+K GV  GRLFYGC  R  +CDFF W   +   HT  A +L W+RF+PPRFK +                  +RQGSVGDCWFL+ LAV++ER DL+ +VVG    +  + ++ G + V LF  GRW ++VVD  LP K P   K  E  L    AF++A NNQ+W  + EKA+AK HGSY AISGG+V +    LTG+ TE +    ND DSE  WA+L+SF+ AGFP+GCAT++         G+V  HAYSVLEVRE+  GV+   Q  + +    G  A   E           + LRL+++RNPWGKREW G++   S+ W+  +   L  +  +DG FWM+++DF+  F  VDVCK+ R W       HS+         R R P   +  + +G                                W ++ ++Q TKRGR +G+WY  L +LV           D G SD   +    G  +R  SC  E+ L++G  Y   ++ + G
Sbjct:  133 SGSYEALATLVCERVGEEGFRDDAFPPAPGSVDGR------------------GNASGNAPQCRCGLQAKVKQVHKDGVTQGRLFYGCEKRVDRCDFFAWCSKETLVHTDAAKALRWKRFAPPRFKFARRT-----------SAADIRQGSVGDCWFLSSLAVIAEREDLLEQVVGVSRRHAGVFEKHGAWFVRLFLGGRWRAIVVDDMLPVKTPKTKKDDEYSL----AFSRAANNQLWVSLTEKAYAKAHGSYSAISGGYVSEGLHDLTGSATEQIDFHANDFDSEECWARLMSFSAAGFPLGCATSFSAE------GIVGHHAYSVLEVREVV-GVKKGVQTKLTEVYARGKDALDVEV----------ENLRLLKIRNPWGKREWRGEFSSNSDAWTKRLGDVLSRTRADDGEFWMSYRDFLMHFSSVDVCKSPRGW-------HSMNLETVLSDTRERGPTFLIRVDDDGGC------------------------------WCHVLLLQHTKRGRPNGHWYTDLSVLVWSR--------DVGSSDWTPMGGMFGARERE-SCQGEIMLDAGLEYAFHILSIAG 625          

BLAST of mRNA_E-siliculosus-1a_M_contig91.17214.1 vs. uniprot
Match: A4S1N6_OSTLU (Calpain catalytic domain-containing protein n=2 Tax=Ostreococcus sp. 'lucimarinus' TaxID=242159 RepID=A4S1N6_OSTLU)

HSP 1 Score: 321 bits (823), Expect = 4.130e-92
Identity = 210/572 (36.71%), Postives = 290/572 (50.70%), Query Frame = 0

Query:  156 YEDGDFLPVPSSLDGRIGRGCSASSRGCTGGGGTETAEGHARCKCGTIARVITVNKAGVNHGRLFYGCI-----TRKCDFFRWA--DDAPHTRQALSLVWRRFSPPRFKLSSAAPRGAEGVGGSFKPEHVRQGSVGDCWFLAGLAVVSERADLIGRVVGSN---GPLADELGCFEVNLFKDGRWESVVVDSWLPCKDPARLKGKEKGLDHLPAFAKAFNNQIWPCIVEKAFAKQHGSYDAISGGHVCDAFEALTGAPTETVVLDND--DSEGNWAQLLSFNQAGFPMGCATAWDPTRGLRDVGLVSTHAYSVLEVRELPPGVRGARQPTIRDWAGLGSAAAAAEQGRRRDGEASGQPLRLVRVRNPWGKREWIGDWGEKSEKWSDSVRAELGWSEKNDGTFWMTWQDFMTRFQLVDVCKARRDWSHASVTNHSIPSPKTCRSFRVRPPPPPMAANTEGQTSSQTTGKNPAATAXXXXXXXXXXPSPTPTTWAYITVVQPTKRGRKDGYWYKALGLLVTEMNPSLSARGDTGHSDGVLVASSLGGAKRSLSC--EVFLESGKAYTASVVCLGG 713
            + D  F   P+S+DGR G      +RG        T E   +C+CG  A+V  V+K GVN GRLFYGC        +CDFF WA  D+  HT  A SLVW+RFS PRFK +                  +RQGSVGDCWFL+ LAVV+ER DL+  V+G +    P+ + +G + + LF  GRW ++VVD ++P K        +K  +  PAF KA  NQ W  IVEKA+AK HGSY AISGG+V +    LTG+ TE +   +   DSE  W +L+SF+ AGFP+GCAT++         G+V  HAYS+LEVRE+  GV+   Q  + +       A   E             LRL+++RNPWGK+EW G++   S  W+  +  EL  +  NDG FW+++ DF+  F  VDVCK+ + W HA     ++  P   RS+ VR      +A   G                                W ++ ++Q TKRGR  G WY  L +LV         R   G S+  +    + GA+   SC  E+ L++G  Y   V+ + G
Sbjct:  141 WMDSGFRAAPASVDGRDG------ARG--------TKENVPKCRCGMQAKVKQVSKDGVNQGRLFYGCAKPAAENTRCDFFAWAKGDNLMHTDAAKSLVWKRFSSPRFKFARRT-----------SAADIRQGSVGDCWFLSSLAVVAEREDLLESVIGVSRRFAPIMERVGAYFIRLFLGGRWRAIVVDDFIPVKP-------KKDDEFAPAFGKASKNQTWVSIVEKAYAKAHGSYAAISGGYVAEGLHDLTGSATEMISFSSTYFDSEEFWVRLVSFSSAGFPLGCATSFSAE------GIVGHHAYSILEVREMH-GVKKGVQTKLPEVLHGKKPAVETEM------------LRLLKIRNPWGKKEWRGEFSSTSASWTKRLGDELSRTRANDGEFWISYNDFLVHFSSVDVCKSPKGW-HALNLETTL-HPDVHRSYIVR------SAGDGG-------------------------------CWCHVLLLQHTKRGRPQGSWYTDLSVLVW--------RRSLGSSEW-MPCGGIFGARERESCQGEIVLDAGTEYAFRVLSIVG 613          

BLAST of mRNA_E-siliculosus-1a_M_contig91.17214.1 vs. uniprot
Match: C1MTG3_MICPC (Predicted protein n=1 Tax=Micromonas pusilla (strain CCMP1545) TaxID=564608 RepID=C1MTG3_MICPC)

HSP 1 Score: 315 bits (806), Expect = 5.600e-90
Identity = 254/682 (37.24%), Postives = 324/682 (47.51%), Query Frame = 0

Query:  136 TAPSAGWEAIAE----------RCQRQGAQYEDGDFLPVPSSLDGRI-GRGCSASSRGCTGGGGTETAEGHARCKCGTI--ARVITVNKAGVNHGRLFYGC----------ITRKCDFFRWADDAPHTRQALSLVWRRFSPPRFKLSSAAPRGAEGVGGSFKPEHVRQGSVGDCWFLAGLAVVSERADLIGRVVGSNGPLADEL------GCFEVNLFKDGRWESVVVDSWLP-----------CKDPARLKGKEKGLDHL-PAFAKAFNNQIWPCIVEKAFAKQHGSYDAISGGHVCDAFEALTGAPTETVVLDNDDSEGN-----------------------------------------------WAQLLSFNQAGFPMGCATAWDPTRGLRDVGLVSTHAYSVLEVRELPPGVRGARQPTIRDWA-----------GLGSAAAAAEQGRRRDGEASGQP-LRLVRVRNPWGKREWIGDWGEKSEKWSDSVRAELGWSEKNDGTFWMTWQDFMTRFQLVDVCKARRDWSHASVTNHSIPSPKTC-RSFRVRPPPPPMAANTEGQTSSQTTGKNPAATAXXXXXXXXXXPSPTPTTWAYITVVQPTKRGRK-DGYWYKALGLLVTEMNPSLSARGDTGHSDGVL--VASSLGGAKRSLSCEVFLESGKAYTASVVCLGG 713
            T PS   EA AE          RC R   +++DG F P  SS+DGR  GRG     +G  GG   E       C C     ARV    + G N GR +Y C            + C+FF WADDAP  + ++   WRR +            GA+GV   F P HVRQG+VGDCW L+ LAV++ER DL  RVVG +             G + V LF  GRW  VVVD  LP              P     K + LD   PAF++A  N++W  +VEKA+AK HGSY AISGG++ +    LTG PTET+ L    SE                                                 W ++LS+ +AGFPMGCAT+     G R  GLV  HAYSVL+VR+L  GV   RQ  +  +             L SA+A+          AS  P LRLVRVRNPWG++EW G+WG KSE W+  + AELG +  +DGTFWM+W DF+  F +VDVCKA R W       H++  P    R+    PP    AA   G  SS+         A XXXXXXXXX       WAY+  +Q TKRGR  + +WY  +G+ V E N    AR +   +D     + + LG  +R    E+ LE GK Y A +  +GG
Sbjct:  119 TTPSQEDEAEAETRDATIEWATRCVRA-PRFDDGSFPPDASSIDGRRKGRG-----KGGKGGEHDEAVVVPRWCACDPPRRARVKRAQRDGPNQGREYYACGLAAARGVFPPPKPCEFFAWADDAPSKKSSVETEWRRLT------------GADGV---FDPAHVRQGAVGDCWLLSALAVIAERQDLARRVVGPSAASPXXXXXXXXSGAYLVRLFLGGRWRGVVVDPCLPVAGRSVEVSRGANGPRPEVNKRRALDDFAPAFSRASGNRLWVPVVEKAYAKAHGSYHAISGGYISEGLLDLTGCPTETIALLGAGSEHYGGETASHTTPFAWCTPFLKDFSRRHSSPALPFQRLTGGGGAFDEARAWEKVLSYCEAGFPMGCATS----SGGR--GLVGGHAYSVLDVRQLH-GVAVGRQTKLDGFVEREVEIVDPAPALVSASASKYASSAXXXXASSPPPLRLVRVRNPWGRKEWNGEWGAKSETWTSRLGAELGNTRADDGTFWMSWHDFLAAFSVVDVCKAHRGW-------HALSLPGAAHRAEGDGPPGTTRAAVDFGMISSEDGDAFEIEVAGXXXXXXXXXXX---XXWAYVMALQHTKRGRGGEAFWYADVGIAVYEKN----ARANAAAADDAWTPLGAVLGAKERVAQAELMLERGKRYLARLFSVGG 758          

BLAST of mRNA_E-siliculosus-1a_M_contig91.17214.1 vs. uniprot
Match: A0A836C9N9_9STRA (Calpain catalytic domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C9N9_9STRA)

HSP 1 Score: 323 bits (828), Expect = 4.040e-89
Identity = 242/693 (34.92%), Postives = 307/693 (44.30%), Query Frame = 0

Query:    4 LFAAATGCLNPLVAASYLERAGYDLTRAVNHFLDSPKNDTAASRGHSDYCRSASCGDDNSGPRSFKRQRAAMAGEKQQQQLLKFEHARPSLSDTSCSSVTASVGMPS-SSGMAEEIHAKPCDSILGAPVPKTTTAPSAGWEAIAERCQRQGAQYEDGDFLPVPSSLDGRIGRGCSASSRGCTGGGGTETAEGHARCKCGTIARVITVNKAGVNHGRLFYGCITRKCDFFRWADDAPHTRQALSLVWRRFSPPRFKLSSAAPRGAEGVGGSFK-------------PEHVRQ----------GSVGDCWFLAGLAVVSERADLIGRVVGSNGPLADELGCFEVNLFKDGRWESVVVDSWLPCKDPAR---------------------LKGKEKG--------LDHLPAFAKAFNNQIWPCIVEKAFAKQHGSYDAISGGHVCDAFEALTGAPTETVVLD--NDDSEGNWAQLLSFNQAGFPMGCATAWDP--TRGLRDVGLVSTHAYSVLEVRELPPGVRGARQPTIRDWAGLGSAAAAAEQGRRRDGEASGQP----LRLVRVRNPWGKREWIGDWGEKSEKWSDSVRAELGWSEKNDGTFWM----------------------------------------TWQDFMTRFQLVDVCKARRDWSHAS---VTNHSIPSPKTCRSFRVRPPP 592
            LF + TGC +P VA  +L  A YDL  AVNH+L S                                  A    E   +  L+ +H  P        SVT     P    G     ++ P       P+       +   + I    QR G  +ED  F PV                                       AR+ TV KAG N  R FY C  RKC+FF+W   A  TRQAL LVWR F+PPRF L  +   GAE +                    HVR           G     +  A LAV+SERADLI  V+ ++ P A   GC+ V LF  G+W SV+VD WLPCK P+                      LK K            +  PAFA A   Q+WP +VEKA+AK HGSY AISGGHV +AF ALTGAPTET++LD  N +SE  W +LLSF  A FPMGCAT WDP  +R  R  GLV+ HAYS+LEVREL  G++  +Q T+  + G  + +++A+   +    AS QP    LRL+R+RNPWG+ EW  DW  KSE+W+  +R EL   E NDGTFWM                                        ++ DF+ RF LVDVCKA   W+H +    T  + P      +F +  PP
Sbjct:  570 LFLSVTGCQHPAVATKFLLGAEYDLNIAVNHYLSSSTTLPDV---------------------------AIQVLEDSDEDDLQAKHPPPQ------PSVTTPRKKPRVQEGGVSSTNSSPSHQQQQLPLLDAAAPVADHLQQIVSHWQRTGQPFEDVSFPPV--------------------------------------AARLSTVRKAGANQNRHFYACALRKCNFFQWCASAAPTRQALQLVWRAFAPPRFCLYKSG--GAEVMLSMHACTTLSAMWLCACVTRHVRSINAMRAIARPGRAMMRYSTACLAVISERADLIQCVLPASEP-ATSAGCYHVRLFLAGKWTSVLVDRWLPCKPPSEPIVAVSPVKPPPKPPRKIGSPLKRKASPSKVKLQYVAERTPAFASAVGGQLWPALVEKAYAKAHGSYHAISGGHVREAFTALTGAPTETLLLDGPNFNSEDAWLRLLSFAHARFPMGCATPWDPDTSRAQRKAGLVNNHAYSILEVRELH-GIKEGKQLTVDHFFGARAESSSAQHMLQ---HASTQPPPPPLRLLRIRNPWGRLEWNADWSAKSEQWTRKLRKELDRGEPNDGTFWMRLPRVHLLAHVEARSTLLEPSVSRAYECVPRVSAATALRCSYHDFLQRFALVDVCKAHEGWTHTTFDTATAAAAPPLACAHAFEIVTPP 1184          

BLAST of mRNA_E-siliculosus-1a_M_contig91.17214.1 vs. uniprot
Match: A0A7S3FQR2_9CHLO (Hypothetical protein n=1 Tax=Chloropicon roscoffensis TaxID=1461544 RepID=A0A7S3FQR2_9CHLO)

HSP 1 Score: 306 bits (783), Expect = 2.530e-88
Identity = 210/534 (39.33%), Postives = 284/534 (53.18%), Query Frame = 0

Query:  149 CQRQGAQYEDGDFLPVPSSLDGR-IGRGCSAS-SRGCTGGGGTETAEGHARCKCGTIARVITVNKAGVNHGRLFYGCITRKCDFFRWADDA-PHTRQALSLVWRRFSPPRFKLSSAAPRGAEGVGGSFKPEHVRQGSVGDCWFLAGLAVVSERADLIGRVVGSNGPLA---DELGCFEVNLFKDGRWESVVVDSWLPCKDPARLKGKEKGLDHLPAFAKA-FNNQIWPCIVEKAFAKQHGSYDAISGGHVCDAFEALTGAPTETVVLD--NDDSEGNWAQLLSFNQAGFPMGCATAWDPTRGLRDV-GLVSTHAYSVLEVRELPPGVRGARQPTIRDW-AGLGSAAAAAEQGRRRDGE-----ASGQ-PLRLVRVRNPWGKREWIGDWGEKSEKWSDSVRAELGWSEKNDGTFWMTWQDFMTRFQLVDVCKARRDWSHASV-TNHSIPSPKT---CRSFRVRPPPPPMAANTEGQTSSQTTGKNPAATAXXXXXXXXXXPSPTPTTWAYITVVQPTKRGR-KDGYWYKALGLLV 660
            C+  GA + D DF P   S+DGR +GR    + S  C             +C   + A++  V K G N GR FYGC TRKC+FFRWA  A  HT QA  L WRRF     K S           G F+ + + QG+ GDCWFLAGL+V++ R DL+ ++     PLA    +LG  +VNLF  G W SV+VD+ LP  +    KG+       PAFAK     ++WP +VEKA+AK  GSY  ISGG V +A   LTG PTE + +D    DSE  +A+LLSF    FP+GC+T     RGLR   GLV  HAYS LEV EL   ++   QP I+D+ +G G   A   + RR + E     A G   LRL+R+RNPWGKREW GD+ ++SEKW++++RA LG + KNDGTFWM W +F+ RF ++++C A + W   S     S PS +    C+ F             E +   +T                          WA+++ +Q +KRGR +D +W+  +   V
Sbjct:  141 CEDAGACFTDPDFPPTAQSIDGRQVGRNFGNNVSIVC-------------QCDPPSRAKLCKVRKDGKNQGRNFYGCATRKCNFFRWAGKALRHTDQASRLTWRRFDGQEHKAS-----------GRFRAQDILQGATGDCWFLAGLSVIAVREDLVRKIF----PLAAQRPDLGLHQVNLFAHGGWSSVIVDNCLPINE----KGE-------PAFAKLPKGTELWPALVEKAYAKLGGSYLGISGGFVSEAMFDLTGFPTEVINMDLPGFDSELTFARLLSFASLRFPIGCSTQ---RRGLRGSDGLVDCHAYSCLEVVELN-NMKVGHQPKIQDFFSGEGGRDA---KRRRAEPEIEAAAADGHGTLRLLRIRNPWGKREWNGDFSDESEKWTNALRARLGKTSKNDGTFWMPWSEFIMRFSILEICHAHQGWHSTSKRVTFSAPSGREWCQCKWF-------------EFEVFDRT--------------------------WAFLSFLQRSKRGRSRDNFWFLDVSFSV 589