prot_E-siliculosus-1a_M_contig8.16166.1 (polypeptide) Ectocarpus siliculosus Ec864m_EcPH12_78m male

BLAST of mRNA_E-siliculosus-1a_M_contig8.16166.1 vs. uniprot
Match: D8LQC0_ECTSI (DNA polymerase epsilon catalytic subunit n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQC0_ECTSI)

HSP 1 Score: 2626 bits (6806), Expect = 0.000e+0
Identity = 1386/1476 (93.90%), Postives = 1390/1476 (94.17%), Query Frame = 0

Query:    1 MAPARGRGKGDWKSNXXXXXXXXXXXXXXXXXSRYQRQQSKXXXXXXXXXXXADSSQAARLSGGGRKESAVKAALREQGDELDKRFGYDRYSEGPPRLGWIFNMLPTSIPDASGNEKSGLDMYFLEQDGGTFKATVFYKPYFYVLVKEERYIQDCIQCIRRRFEDSAVEVTVVDKEDLDMPNHLSGKLRRFLKLSFHSVADLMEVKRALMPTIQANKTRAEAQDAYMHAEAREQQPSDFLSVLVDAREYDVPYYVRCSIDLDIRVGAWYMVTPQEEGVEVAWQKEMVDKAEPKVLAYDIECTKAPLKFPNVEIDQVFMISYMVDGQGYLIISREVVSEDVENFEYTPKPSFPGPFHIFNEPNEEAVIRRFFNHVAELRPQIIVTYNGDFFDWPFVDERAKGYGLDMEKEIGVGLQANGEYRGRCVAHMDAIYWVKRDSYLPQGSHGLKAVTKYKLGYDPVEVDPEDMVRYASERPTEMAAYSVSDAVATYYLYQTYVHMFVFSLCTIIPMGPDDVLRKGSGTLCEALLMVEAYRGNIVCPNKHSEPLTKFHDGHLVESETYIGGHVECLETGVFRSDLPEKFRLVPSALQALIDNVDRDLTFAIEVESGVQRDTVSNYDEVRSEIIEQLEMLRDSPLREEEPFIYHLDVAAMYPNIILSNRLQPGAVVNKAMCASCDFNQESNNCKRSLTWKWRGDMTPASRTEYQSVKMQLTYENVDGKPFVELDEADQATNVRARLKNYAHKVYKKTKITKEEERVDTVCMRENPFYVNTVRAFRDRRYDYKILTKKAKKAKSKAAEEGDAVGAKEAGDRAQVFDSLQLAHKCILNSFYGYVMRKGARWRSMEMAGIVTYTGAMLIKQARELVEQIGRPLELDTDGIWCILPSSFPENFTFTTKSGGKIRISYPCVMLNADVHENYTNHQYQDLQDPATRKYNTKSECSIFFEVDGPYRCMVLPSSTEEGKLLKKRYAVFNHDGSLAELKGFELKRRGELEVIKVFQSQVFEKFLRGDTLAQCYDAVGEVANQWLDVLYSKGGDVDDDELMELISQNKTMSQTLEDYGTAKGTSQTTARRLADFLGAEMVKDKGLNCRMILSNRPHGAPVTDRAVPTAIFSAEPAVKKHYLRKWLKDPAMEDFDIRSILDWDYYLDRLGNCIRKIITIPAAMQKVENPCPRVKHPDWLRKIVREINDGYRQMKIDQLFKAAPPGTPQLTNAGASTKAKPTPAPAATDIEDMAGXXXXX--AAGRSILGARARVTKRIGNGAAAAGALAPDGSPGKENADVGXXXXXXXXXXXXXX-PIRREDDFQGWLAQQKAGWKKRREQNRLKRRTEARSQAMASFRGSGSAGARGGTAGLGAAGAAEKRRRTAAMGKSGLQGYLTSAQDALLRGFWQVVEIRPSDNPGSFTVFAMTGPGELQVIPITIPRVFYVNCLEDKGPAGSSTMGRRVARTLPNGRGSPFLYEVAMDEAKFQRNEKQ 1473
            MAPARGRGKGDWK N XXXXXXXXXXXX    SRYQRQQSKXXXXXXXXXXX      ARLS  GRKESAVKAALREQGD LDKRFGYDRYSEGPPRLGWIFNMLPTSIPDASGNEKSGLDMYFLEQDGGTFKATVFYKPYFYVLVKEERYIQDCIQCIRRRFEDSAVEVTVVDKEDLDMPNHLSGKLRRFLKLSFHSVADLMEVKRALMPTIQANKTRAEAQDAYMHAEAREQQPSDFLSVLVDAREYDVPYYVRCSIDLDIRVGAWYMVTPQEEGV+VAWQKEMVDKAEPKVLAYDIECTKAPLKFPNVEIDQVFMISYMVDGQGYLIISREVVSEDVENFEYTPKPSFPGPFHIFNEPNEEAVIRRFF HVAELRPQIIVTYNGDFFDWPFVDERAKGYGLDMEKEIGVGLQANGEYRGRCVAHMDAIYWVKRDSYLPQGSHGLKAVTKYKLGYDPVEVDPEDMVRYASERPTEMAAYSVSDAVATYYLYQTYVHMFVFSLCTIIPMGPDDVLRKGSGTLCEALLMVEAYRGNIVCPNKHSEPLTKFHDGHLVESETYIGGHVECLETGVFRSDLPEKFRLVPSALQALIDNVDRDLTFAIEVESGVQRDTVSNYDEVRSEIIEQLEMLRDSPLREEEPFIYHLDVAAMYPNIILSNRLQPGA+VNKAMCASCDFNQ+SNNCKRSLTWKWRGDMTPASRTEYQSVKMQLTYENVDGKPFVELDEADQATNVRARLKNYAHKVYKKTKITKEEERVDTVCMRENPFYVNTVRAFRDRRYDYKILTKKAKKAKSKAAEEGDAVGAKEAGDRAQVFDSLQLAHKCILNSFYGYVMRKGARWRSMEMAGIVTYTGAMLIKQARELVEQIGRPLELDTDGIWCILPSSFPENFTFTTKSGGKIRISYPCVMLNADVHENYTNHQYQDLQDPATRKYNTKSECSIFFEVDGPYRCMVLPSSTEEGKLLKKRYAVFNHDGSLAELKGFELKRRGELEVIKVFQSQ                                                              GTSQTTARRLADFLGAEMVKDKGLNCRMILSNRPHGAPVTDRAVPTAIFSAEPAVKKHYLRKWLKDPAMEDFDIRSILDWDYYLDRLGNCIRKIITIPAAMQKVENPCPRVKHPDWLRKIVREINDGYRQ KIDQLFKAAPPGTPQLTNAGASTKAKPTPAPAATDIEDMAGXXXXX  AAGRSILGARARVTKRIGNGAAA GALAPDGSPGKENADVGXXXXXXXXXXXXX  PIRREDDFQGWLAQQKAGWKKRREQNRLKRRTEARSQAMASFRGSGS+GARGGTAGLGAAGAAEKRRRTAAMGKSGLQGYLTSAQDALLRGFWQVVEIRPSDNPGSFTVFAMTGPGELQVIPITIPRVFYVNCLEDKGPAGSS MGRRVARTLPNG GSPFLYEVAMDEAKFQRNEKQ
Sbjct:    1 MAPARGRGKGDWKRNGXXXXXXXXXXXX----SRYQRQQSKXXXXXXXXXXXXXXXXXARLSXXGRKESAVKAALREQGDALDKRFGYDRYSEGPPRLGWIFNMLPTSIPDASGNEKSGLDMYFLEQDGGTFKATVFYKPYFYVLVKEERYIQDCIQCIRRRFEDSAVEVTVVDKEDLDMPNHLSGKLRRFLKLSFHSVADLMEVKRALMPTIQANKTRAEAQDAYMHAEAREQQPSDFLSVLVDAREYDVPYYVRCSIDLDIRVGAWYMVTPQEEGVDVAWQKEMVDKAEPKVLAYDIECTKAPLKFPNVEIDQVFMISYMVDGQGYLIISREVVSEDVENFEYTPKPSFPGPFHIFNEPNEEAVIRRFFKHVAELRPQIIVTYNGDFFDWPFVDERAKGYGLDMEKEIGVGLQANGEYRGRCVAHMDAIYWVKRDSYLPQGSHGLKAVTKYKLGYDPVEVDPEDMVRYASERPTEMAAYSVSDAVATYYLYQTYVHMFVFSLCTIIPMGPDDVLRKGSGTLCEALLMVEAYRGNIVCPNKHSEPLTKFHDGHLVESETYIGGHVECLETGVFRSDLPEKFRLVPSALQALIDNVDRDLTFAIEVESGVQRDTVSNYDEVRSEIIEQLEMLRDSPLREEEPFIYHLDVAAMYPNIILSNRLQPGAIVNKAMCASCDFNQDSNNCKRSLTWKWRGDMTPASRTEYQSVKMQLTYENVDGKPFVELDEADQATNVRARLKNYAHKVYKKTKITKEEERVDTVCMRENPFYVNTVRAFRDRRYDYKILTKKAKKAKSKAAEEGDAVGAKEAGDRAQVFDSLQLAHKCILNSFYGYVMRKGARWRSMEMAGIVTYTGAMLIKQARELVEQIGRPLELDTDGIWCILPSSFPENFTFTTKSGGKIRISYPCVMLNADVHENYTNHQYQDLQDPATRKYNTKSECSIFFEVDGPYRCMVLPSSTEEGKLLKKRYAVFNHDGSLAELKGFELKRRGELEVIKVFQSQ--------------------------------------------------------------GTSQTTARRLADFLGAEMVKDKGLNCRMILSNRPHGAPVTDRAVPTAIFSAEPAVKKHYLRKWLKDPAMEDFDIRSILDWDYYLDRLGNCIRKIITIPAAMQKVENPCPRVKHPDWLRKIVREINDGYRQTKIDQLFKAAPPGTPQLTNAGASTKAKPTPAPAATDIEDMAGXXXXXXXAAGRSILGARARVTKRIGNGAAAGGALAPDGSPGKENADVGXXXXXXXXXXXXXAVPIRREDDFQGWLAQQKAGWKKRREQNRLKRRTEARSQAMASFRGSGSSGARGGTAGLGAAGAAEKRRRTAAMGKSGLQGYLTSAQDALLRGFWQVVEIRPSDNPGSFTVFAMTGPGELQVIPITIPRVFYVNCLEDKGPAGSSAMGRRVARTLPNGHGSPFLYEVAMDEAKFQRNEKQ 1410          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16166.1 vs. uniprot
Match: A0A6H5J9K7_9PHAE (DNA polymerase epsilon catalytic subunit n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5J9K7_9PHAE)

HSP 1 Score: 2459 bits (6374), Expect = 0.000e+0
Identity = 1433/1636 (87.59%), Postives = 1451/1636 (88.69%), Query Frame = 0

Query:  953 MVLPSSTEEGKLLKKRYAVFNHDGSLAELKGFELKRRGELEVIKVFQSQVFEKFLRGDTLAQCYDAVGEVANQWLDVLYSKGGDVDDDELMELISQNKTMSQTLEDYGTAK-----------------GTSQTTARRLADFLGAEMVKDKGLNCRMILSNRPHGAPVTDRAVPTAIFSAEPAVKKHYLRKWLK--------------DPAMEDFD-IRSILDWDYYLDRLGNCIRKIITIPAAMQKVENPCPRVKHPDWLRKIVREINDGYRQMKIDQLFKAAPPGTPQLTNAGASTKAKPTPAPAATDIEDMA---GXXXXXAAGRSILGARARVTKRIGNGAAAAGALAPDGSPGKENADVGXXXXXXXXXXXXXXPIRREDDFQGWLAQQKAGWKKRREQNRLKRRTEARSQAMASFRGSGSAGARGGTAGLGAAGAAEKRRRTAAMGKSGLQGYLTSAQDALLRGFWQVVEIRPSDNPGSFTVFAMTGPGELQVIPITIPRVFYVNCLEDKGPAGSSTMGRRVARTLPNGRGSPFLYEVAMDEAKFQRNEKQVEDFLHHAGIEGVYELGTPLVYRAMLHLGSVARVVQGRAKALKGVKSGSGQAPGFELEDLELVTAAAHPYLHPSVATFKHIYLYHSSSGKRGMVGLFYVQGTNEDIEPEPTAEGGAEDVGGEGGLGPGERPVIAKAVVWLVNPFSRRDGEQRPPFARIFEKFT----------NNPDSSCTFKTSSVSSADEAWAAADASLASYSRQRNGPTLVLSQGLVQGQELRRRLPALSELPVTHIPGNAEDERYPALGWQAFAAQRMVQRFFIAPRWLADRLLCARYAHLPLANIGADAPCAMADAFFGRLLRHNRHVLWASETSRPDLGGTEGDDNDIWAEDLASPTILQPGAYRNICIELEIHGLAVGSVIASSQLEALEGSQGVTALTVNNDNEGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-QAVASAFRILKVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNATLHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQLEPKMFWEQLLFLDDQDYGGIQVQGGFKEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKRDNDLKPXXXXXXEDAQVSGRGVSGADGTVAEQKMPSIVSHWNLAEYLPASVRQVFLILVSTFLLKPHEKARQLRLDHERMVASLSNQLTQSSQPNGPASPGTDRGESXXXXXXXXXVPASQGGMDAALAVQAALRKEEANESQSMADFLKHLVTRTLTDKVLETVPAIKKYSGSGPDAFPNKPGAARELTNPALEFIKSLCHVLSLDPTVTDEVETLRKQALAEVGVRQFSAEGQYSDPCVSFVLPDVICSFCNLCRDLDLCRDSRLMDEDEDERWQCIQCNHPYERSSIELSLVEAVQRRSVRYQLQDMKCAKCGAVATRSMSLTCPCSGPLVAEESQQEFHKTMKLLRQLAIFHNFGWLLETVENILVADGLVEEDVPVQDGGEXXXXXXXREAMDEDGHAAIDG 2542
            MVLPSSTEEGKLLKKRYAVFNHDGSLAELKGFELKRRGELEVIKVFQSQVFEKFLRGDTLAQCYDAVGEVANQWLDVLYSKGGDVDDDELMELISQNKTMSQTLEDYGTAK                 GTSQTTARRLADFLGAEMVKDKGLNCRMILSNRPHGAPVTDRAVPTAIFSAEPAVKKHYLRKWLK              D  +ED + +     W+      G+   K +       KVENPCPRVKHPDWLRKIVREINDGYRQ KIDQLFKAAPPGTPQLTNAGASTKAKPTPA A+TDIEDM    G     AAGRSILGARARVTKRIGNG  A GALAP GSPGKENADV      XXXXXXX  PIRREDDFQGWLAQQKAGWKKRREQNRL    EARSQAMASFRGSGSAGARGGTAGLGAAG AEKRRRTAAMGKSGLQGYLTSAQDALLRGFWQVVEIRPSD+PGSFTVFAMTGPGELQVIPITIPRVFYVNCLEDKGPAGSS MGRRVARTLPNGR SPFLYEVAMDEAKFQRNEKQVEDFLHHAGIEGVYELGTPLVYRAMLHLGSVARVVQGRAKALKGVKSGSGQAPGFELEDLELVTAAAHPYLHPSVATFKHIYLYHSSSGKRGMVGLFYVQGTNEDIEPEPTAEGGAEDVGGEGGLGPGERPVIAKAVVWLVNPFSRRDGEQRPPF+RIFEKFT          +NPDSSCTFKTSSVSSADEAWAAADASLASYSRQRNGPTLVLSQGL+QGQELRRRLPALSELPVTHIPGNAEDERYPALGWQAFAAQRMVQRFFIAPRWLADRLLCARYAHLPLANIGADAPCAMADAFFGRLLRHNRHVLWASETSRPDLGG EGDDNDIWAEDLASPTILQPGAYRNICIELEIHGLAVGSVIASSQLEALEGSQGVTALTVNNDNEGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX QAVASAFRILKVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNATLH LV       FSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFV+STVTSRDLFNTLQLEPKMFWEQLLFLDDQDYGGIQVQG F+EXXXXXXXXXXXXXXXXXXXXXXXXXXXXX                XXXXXXXXXXXXXXXXXXXXXXXXX                        KRDNDLKP   XXX          SG  GT+AE+KMPSIVSHWNLAEYLPASVRQVFLILVSTFLLKPHEKARQLRLDHERMVASLSNQLTQ SQPNGPA PGTDRG S         VPASQG MDAALAVQAALRKEEANESQSMADFLKHLVTRTLTDKVLETVPAIKKYSGSGPDAFPNKPGAARELTNPALEFIKSLCHVLSLDPTVTDEVETLRKQALAEVGVRQFSAEGQYSDPCVSFVLPDVICSFCNLCRDLDLCRD+RLMDEDE+ERWQCIQCNHPYERSSIELSLVEAVQRRSVRYQLQDMKCAKCGAVATRSMSLTCPCSGPLVAEESQQ+FHKTMKLLRQLAIFHNFGWLLETV NILVADGLVEE VP Q GG   XXXX  EAMDEDGHAAIDG
Sbjct:    1 MVLPSSTEEGKLLKKRYAVFNHDGSLAELKGFELKRRGELEVIKVFQSQVFEKFLRGDTLAQCYDAVGEVANQWLDVLYSKGGDVDDDELMELISQNKTMSQTLEDYGTAKKLLRSPHKQLLFLLSPQGTSQTTARRLADFLGAEMVKDKGLNCRMILSNRPHGAPVTDRAVPTAIFSAEPAVKKHYLRKWLKVDMHGIGVGGGDVNDGVVEDDNGLTEPFVWES--PTKGHYFCKFL-------KVENPCPRVKHPDWLRKIVREINDGYRQTKIDQLFKAAPPGTPQLTNAGASTKAKPTPAAASTDIEDMTVAGGGCGGGAAGRSILGARARVTKRIGNGTPAGGALAPGGSPGKENADV-RGDEQXXXXXXXVVPIRREDDFQGWLAQQKAGWKKRREQNRLXXXXEARSQAMASFRGSGSAGARGGTAGLGAAGVAEKRRRTAAMGKSGLQGYLTSAQDALLRGFWQVVEIRPSDHPGSFTVFAMTGPGELQVIPITIPRVFYVNCLEDKGPAGSSIMGRRVARTLPNGRASPFLYEVAMDEAKFQRNEKQVEDFLHHAGIEGVYELGTPLVYRAMLHLGSVARVVQGRAKALKGVKSGSGQAPGFELEDLELVTAAAHPYLHPSVATFKHIYLYHSSSGKRGMVGLFYVQGTNEDIEPEPTAEGGAEDVGGEGGLGPGERPVIAKAVVWLVNPFSRRDGEQRPPFSRIFEKFTKCDSASVINTSNPDSSCTFKTSSVSSADEAWAAADASLASYSRQRNGPTLVLSQGLLQGQELRRRLPALSELPVTHIPGNAEDERYPALGWQAFAAQRMVQRFFIAPRWLADRLLCARYAHLPLANIGADAPCAMADAFFGRLLRHNRHVLWASETSRPDLGGREGDDNDIWAEDLASPTILQPGAYRNICIELEIHGLAVGSVIASSQLEALEGSQGVTALTVNNDNEGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQAVASAFRILKVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNATLHCLVHRLMHKVFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVISTVTSRDLFNTLQLEPKMFWEQLLFLDDQDYGGIQVQGDFEEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFSEEGSEDDAEGLQDSXXXXXXXXXXXXXXXXXXXXXXXXXFKDRNGGGSGSRKDKAVPKA----KRDNDLKPDEEXXXXXXXXXXXXXSGTVGTMAERKMPSIVSHWNLAEYLPASVRQVFLILVSTFLLKPHEKARQLRLDHERMVASLSNQLTQWSQPNGPAPPGTDRGGSGGGG-----VPASQGEMDAALAVQAALRKEEANESQSMADFLKHLVTRTLTDKVLETVPAIKKYSGSGPDAFPNKPGAARELTNPALEFIKSLCHVLSLDPTVTDEVETLRKQALAEVGVRQFSAEGQYSDPCVSFVLPDVICSFCNLCRDLDLCRDARLMDEDEEERWQCIQCNHPYERSSIELSLVEAVQRRSVRYQLQDMKCAKCGAVATRSMSLTCPCSGPLVAEESQQDFHKTMKLLRQLAIFHNFGWLLETVANILVADGLVEESVPAQ-GGREEXXXXEGEAMDEDGHAAIDG 1616          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16166.1 vs. uniprot
Match: W7TVX1_9STRA (DNA-directed DNA polymerase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TVX1_9STRA)

HSP 1 Score: 2305 bits (5974), Expect = 0.000e+0
Identity = 1332/2546 (52.32%), Postives = 1678/2546 (65.91%), Query Frame = 0

Query:   53 ADSSQAARLSGGGRKESAVKAALREQGDELDKRFGYDRYSEGPPRLGWIFNMLPTSIPDASGNEKSGLDMYFLEQDGGTFKATVFYKPYFYVLVKEERYIQDCIQCIRRRFEDSAVEVTVVDKEDLDMPNHLSGKLRRFLKLSFHSVADLMEVKRALMPTIQANKTR------AEAQDAYMHA---EAREQQPSDFLSVLVDAREYDVPYYVRCSIDLDIRVGAWYMVTPQEEGVEVAWQKEMVDKAEPKVLAYDIECTKAPLKFPNVEIDQVFMISYMVDGQGYLIISREVVSEDVENFEYTPKPSFPGPFHIFNEPNEEAVIRRFFNHVAELRPQIIVTYNGDFFDWPFVDERAKGYGLDMEKEIGVGLQANGEYRGRCVAHMDAIYWVKRDSYLPQGSHGLKAVTKYKLGYDPVEVDPEDMVRYASERPTEMAAYSVSDAVATYYLYQTYVHMFVFSLCTIIPMGPDDVLRKGSGTLCEALLMVEAYRGNIVCPNKHSEPLTKFHDGHLVESETYIGGHVECLETGVFRSDLPEKFRLVPSALQALIDNVDRDLTFAIEVESGVQRDTVSNYDEVRSEIIEQLEMLRDSPLREEEPFIYHLDVAAMYPNIILSNRLQPGAVVNKAMCASCDFNQESNNCKRSLTWKWRGDMTPASRTEYQSVKMQLTYENVD-GKPFVELDEADQATNVRARLKNYAHKVYKKTKITKEEERVDTVCMRENPFYVNTVRAFRDRRYDYKILTKKAKKAKSKAAEEGDAVGAKEAGDRAQVFDSLQLAHKCILNSFYGYVMRKGARWRSMEMAGIVTYTGAMLIKQARELVEQIGRPLELDTDGIWCILPSSFPENFTFTTKSGGKIRISYPCVMLNADVHENYTNHQYQDLQDPATRKYNTKSECSIFFEVDGPYRCMVLPSSTEEGKLLKKRYAVFNHDGSLAELKGFELKRRGELEVIKVFQSQVFEKFLRGDTLAQCYDAVGEVANQWLDVLYSKGGDVDDDELMELISQNKTMSQTLEDYGTAKGTSQTTARRLADFLGAEMVKDKGLNCRMILSNRPHGAPVTDRAVPTAIFSAEPAVKKHYLRKWLKDPAMEDFDIRSILDWDYYLDRLGNCIRKIITIPAAMQKVENPCPRVKHPDWLRKIVREINDGYRQMKIDQLFKAAPPGTPQLTNAGASTKAKPTPAPAATDIEDMAGXXXXXAAGRSILGARARVTKRIGNGAAAAGALAPDGSPGKENADVGXXXXXXXXXXXXXXPIRRE--------------------DDFQGWLAQQKAGWKKRREQNRLKRRTEARSQ--AMASFRGSGSAGARGGTAGLGAAGAAEKRRRTAAMGKSGLQGYLTSAQDALLRGFWQVVEIRPSDNPGSFTVFAMTGPGELQVIPITIPRVFYVNCLEDKGPAGSSTMGRRVARTLPNGRGSPFLYEVAMDEAKFQRNEKQVEDFLHHAGIEGVYELGTPLVYRAMLHLGSVARVVQGRAKALKGVKSGSGQAPGFELEDLELVTAAAHPYLHPSVATFKHIYLYHSSSGKRGMVGLFYVQGTNEDIEPEPTAEGGAEDVGGEGGLGPGERPVIAKAVVWLVNPFSRRDGEQRPPFARIFEKFTNNPDSSCTFKTSSVSSADEAWAAADASLASYSRQRNGPTLVLSQGLVQGQELRRRLPALSELPVTHIPGNAEDERYPALGWQAFAAQRMVQRFFIAPRWLADRLLCARYAHLPLANIGADAPCAMADAFFGRLLRHNRHVLWASETSRPDLGGTEGDDNDIWAEDLASPTILQPGAYRNICIELEIHGLAVGSVIASSQLEALEGSQGVTALTVNNDNEGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQAVASAFRILKVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNATLHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQLEPKMFWEQLLFLDDQDYGGIQVQGGFKEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-----------------------------------------------------------------KRDNDLKPXXXXXXEDAQVSGRGVSGADGTVAEQKMPSIVSHWNLAEYLPASVRQVFLILVSTFLLKPHEKARQLRLDHERMVASLSNQLTQSSQPNGPASPGTDRGESXXXXXXXXXVPASQGGMDAALAVQAALRKEEANESQSMADFLKHLVTRTLTDKVLETVPAIKKYSGSGPDAFPNKPGAARELTNPALEFIKSLCHVLSLDPTVTDEVETLRKQALAEVGVRQFSAEGQYSDPCVSFVLPDVICSFCNLCRDLDLCRDSRLMDEDEDERWQCIQCNHPYERSSIELSLVEAVQRRSVRYQLQDMKCAKCGAVATRSMSLTCPCSGPLVAEESQQEFHKTMKLLRQLAIFHNFGWLLETVE 2501
            A++SQAARL    +K SA +   RE GDE D RFGY R+ EG PRLGW+ +M  +++PD  G E+SG+D+YFL+QDG TFK T FY+PYFY+ ++++RYI + +Q ++R+F+   + +   DKEDLD+PNHLSGKL RFL+LSF +V++LMEV+  L+P ++ NK R       +A++  M      A  + P DF   LVD REYDVPY VR +IDLD+RVGAWY VT + E V + W+K++++KAEP+VLA+DIECTKAPLKFP+  +DQ+FMISYMVDGQGYL+I+REVV ED+++FEYTPKP++ GPF + N P+EEAV+R F  HV ELRPQI VTYNGD+FDWPF++ R + YG++M  EIGV  ++ GEYRGR   H+DA +WVKRDSYLPQG+ GLKAVTKYKLGYDPVEVDPEDMVR+A ER TEMA+YSVSDAVATYYLY TYVH F+FSL TIIPM  +DVLRKGSGTLCE LLMVEA+RGNIVCPNK  E   +FH GHL+ESETY+GGHVECLETGV+RSD+  KFRLVPSALQALIDNVDRDL FA+EVE   + + V NY EV++EIIE+L  LRD+PLR+E P IYHLDV AMYPNIIL+NRLQP ++V+++ CA+CDFNQE N CKR + W WRGD  PA+R++++ V++QL YE    G+ +  LD A+Q   +R RLK Y+HKVYKKTK+T +EER DTVCMRENPFYVNTVRAFRDRRY+YK+LTKK K  K KA    DA+G K A D+  VFDSLQLAHKCILNSFYGYVMRKGARWRSMEMAGIVT TGA LI QAREL+EQIGRPLELDTDGIWCILP SFPENFT  T +G KI +SYPCVMLNADVHE YTNHQYQDL DPAT +Y T SECSIFFEVDGPYRCMVLPSSTEEGKLLKKRYAVFN DGSLAELKGFELKRRGELE+IK FQ QVFE FL+G TL +CY+AVG++AN WLDVL S+G ++DD+E++ LIS+NKT+S+TL+ YG  KG S TTA RLADFLG +MVKDKGLNCR++++NRPHGAPVT+RA+PTAIFS  P V+KH+LRKWLKD AM DFDIRS++DWDYY +RLG  I+KIITIPAAMQKV NP PRV HPDWL K +RE+ DGYRQ+KI  LF A      +    G  T  K     A  DIED  G         S L     V +R G        L  +G    ++ +                PI+                      +DFQGWLA +KA W+    + R++RR EAR +  A+ + R     G   GT G+       KR+  A+        +L  A  A+ +G WQV+EIR S+ PG F V+AMTG   +Q + + + R  Y+    D         GRRV R LP+G  SP+ YEV MDE +F  NE  +  +L  AG+ GVYEL TPL +RA+  +G +A+V         G KSGS     F LEDLE ++ A+ PYLHPS A F+++Y+Y S S +R +V LF + G N D+E E  +   A  V            + A A VWL NPFS    E RPP  R F ++   P  +CTF ++ V+S   A A  +A+L  Y ++R+GPT+VLSQ ++    LRR +PAL++LPV  +P NA+D RYPAL WQ+++ QRM+QRF   P WL DRL  ARY+HLPL N+G D    MAD   GRLL  NRHVLWASE +RPDLGG E D ND WA++ ++P I  PGAYR +CIELEI GLAV S++++ +LE ++G+QG+    V   N G                                   +A ASAFR+L+VLV TW+ EV  +G+  AD+L  +F+RW+C   SL ++  L RL        F RL+AE+RRLGA V+FA+ Q+++IATNK D+ +A +YA FV+ TV SR +F+ LQ+ P  +W QL F D+ ++GG+    G+K+            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX      XXXXXXXXXXXXXXXX   XXXXXXXXXXXXXXXXXXXXXXXXX                                                                 +  + L+       +D   S RG  GA+  V E+    + SHW++  YLPA++R  F  +V+T L     + R  +   +  +A+L N+L            GT +G+                           L+KEE N  +SM  FL+ +V   LT  +L TV  I      GP+ FP   G  R+ T PALEF+K +C +L LD  V ++V  LR+Q LA++G ++FS +G  +D C SFV+PD+IC+FC+ CRDLDLCRD +L+ ED D  W+C  C + Y+   +E  L+ AV+R+  RY LQD++C  C  V+T SMS  C CSG LV +E   EF   M  LR++A+ ++   L E V+
Sbjct:   35 ANASQAARLQKS-QKASASRYRAREAGDEFDIRFGYSRFEEGSPRLGWLLDMNASTVPDEEGRERSGMDLYFLQQDGDTFKVTTFYEPYFYIGLRDDRYIAEVVQLLQRKFDQVGMTILACDKEDLDLPNHLSGKLHRFLRLSFFNVSELMEVRSVLLPLVERNKQRMAVGLAGDAREGGMEGLGPGAGARPPDDFFETLVDIREYDVPYVVRVAIDLDLRVGAWYTVTARGEHVALKWEKDIIEKAEPRVLAFDIECTKAPLKFPDANVDQIFMISYMVDGQGYLLINREVVGEDIQDFEYTPKPAYAGPFTVVNVPDEEAVLREFIRHVQELRPQIFVTYNGDYFDWPFMETRMEAYGIEMSAEIGVA-ESQGEYRGRAAVHLDAFHWVKRDSYLPQGAQGLKAVTKYKLGYDPVEVDPEDMVRFAHERATEMASYSVSDAVATYYLYTTYVHNFIFSLSTIIPMKAEDVLRKGSGTLCETLLMVEAFRGNIVCPNKQVEAPMRFHKGHLLESETYVGGHVECLETGVYRSDIDYKFRLVPSALQALIDNVDRDLRFAVEVEGHARVENVVNYAEVKAEIIERLAELRDTPLRDEPPLIYHLDVGAMYPNIILTNRLQPCSIVDQSDCAACDFNQEKNQCKRPMKWVWRGDHLPATRSDFEQVQVQLAYERTSTGEAYHALDRAEQTRKLRERLKLYSHKVYKKTKVTVQEEREDTVCMRENPFYVNTVRAFRDRRYEYKVLTKKWKSVKLKAEASDDALGRKAAEDKETVFDSLQLAHKCILNSFYGYVMRKGARWRSMEMAGIVTDTGARLITQARELMEQIGRPLELDTDGIWCILPVSFPENFTLLTAAGKKIPLSYPCVMLNADVHERYTNHQYQDLVDPATHRYATHSECSIFFEVDGPYRCMVLPSSTEEGKLLKKRYAVFNKDGSLAELKGFELKRRGELELIKAFQGQVFECFLKGSTLEECYNAVGDIANFWLDVLDSRGEELDDEEVVGLISENKTISKTLDQYGDQKGASLTTASRLADFLGPDMVKDKGLNCRLVIANRPHGAPVTERAIPTAIFSTAPGVRKHFLRKWLKDSAMSDFDIRSLIDWDYYKERLGKSIQKIITIPAAMQKVPNPVPRVLHPDWLDKKIRELTDGYRQLKISNLFAAE-----KNAKGGVGTPGKH----ALMDIEDTMGGLSRGKG--SPLKPYTAVVRRAGR------KLDEEGHDENDSTEANGKIGQDAAVEDVPRPIQEAKAVGESAGMPASRRRCPAIGEDFQGWLAFRKAQWR----EGRMERRLEARGRRGAVGNRRPRHGEGVEPGTQGV------TKRQNVAS--------FLKGAALAVTQGCWQVIEIRESELPGEFVVWAMTGQKSMQRLHLKVHRTMYITSNMDDEVGFQKIKGRRVTRFLPHGYRSPYTYEVTMDERRFLSNENNLARWLSTAGVTGVYELQTPLWFRAIAKVGCLAQVSSRYQGGAGGGKSGSR---AFRLEDLESLSTASMPYLHPSTAVFRNLYVYISQSARRALVALFVLGGDNRDLELE--SADAANSV----------HSMRATAQVWLANPFST--AEARPPLKRFFARYAP-PGVACTFSSTYVASLKSALAQLNAALTGYIQERHGPTVVLSQSVLDLSILRRSVPALNDLPVVSMPSNADDNRYPALSWQSYSTQRMIQRFLCMPGWLQDRLQAARYSHLPLGNLGLDPQRTMADVLLGRLLHGNRHVLWASEGTRPDLGGAEDDQNDAWADEASNPVIRVPGAYRKVCIELEIEGLAVLSMLSAVELEEMDGAQGLATSDVAQGNGG-------------------VAGPGGESAGAGGGDDRACASAFRLLRVLVETWMGEVERVGSLQADALATNFWRWMCDADSLFYDPALRRLCLMLINKYFHRLVAEMRRLGAQVIFANAQRIMIATNKEDMASAHEYASFVLRTVLSRRIFSRLQITPVRYWWQLFFFDEHNFGGL----GYKDPEAAAAHRAALKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKVRRKMXXXXXXXXXXXXXXXXDGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEVVGSQSSTEGTGSALVQGRAKSRLEEVGEEMGDDGAGSLRGEEGAEADVDEEDY-DLQSHWDMLNYLPAALRHPFDYVVATMLADHLREERDRKEHRKERLAALGNRLL-----------GTSQGQEAEAEEEVEM-----------------LQKEEENHERSMVRFLQKMVEGRLTRTLLRTVQDIDNEF-RGPECFPATVGVHRQSTTPALEFVKVVCGLLKLDSAVEEQVVVLRRQLLAQLGAKEFSEQGLSADYCRSFVVPDLICAFCSHCRDLDLCRDEQLLSEDPDRWWRCPHCANRYDVDRVEQLLITAVERQVTRYALQDLRCVNCRRVSTLSMSDVCKCSGRLVGDEKPSEFQDLMATLRRIALQYSLAGLKEAVD 2472          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16166.1 vs. uniprot
Match: A0A3R7G7W5_9STRA (DNA polymerase epsilon catalytic subunit n=5 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A3R7G7W5_9STRA)

HSP 1 Score: 1774 bits (4594), Expect = 0.000e+0
Identity = 1079/2584 (41.76%), Postives = 1454/2584 (56.27%), Query Frame = 0

Query:   78 QGDELDKRFGYDRYSE-------------------GPPRLGWIFNMLPTSIPDA--SGNEKSGLDMYFLEQDGGTFKATVFYKPYFYVLVKEERYIQDCIQCIRRRFEDSAVEVTVVDKEDLDMPNHLSGKLRRFLKLSFHSVADLMEVKRALMPTIQANKTRAEAQDAY-MHAEAREQQPSDFL-------------------SVLVDAREYDVPYYVRCSIDLDIRVGAWYMV----TPQEEGVEVAWQKEMVDKAEPKVLAYDIECTKAPLKFPNVEIDQVFMISYMVDGQGYLIISREVVSEDVENFEYTPKPSFPGPFHIFNEPNEEAVIRRFFNHVAELRPQIIVTYNGDFFDWPFVDERAKGYGLDMEKEIGVGL-QANGEYRGRCVAHMDAIYWVKRDSYLPQGSHGLKAVTKYKLGYDPVEVDPEDMVRYASERPTEMAAYSVSDAVATYYLYQTYVHMFVFSLCTIIPMGPDDVLRKGSGTLCEALLMVEAYRGNIVCPNKHSEPLT-KFHDGHLVESETYIGGHVECLETGVFRSDLPEKFRLVPSALQALIDNVDRDLTFAIEVESGVQRDTVSNYDEVRSEIIEQLEMLRDSPLREEEPFIYHLDVAAMYPNIILSNRLQPGAVVNKAMCASCDFNQESN---------NCKRSLTWKWRGDMTPASRTEYQSVKMQLTYENV----------------DGK--------PFVELDEADQATNVRARLKNYAHKVYKKTKITKEEERVDTVCMRENPFYVNTVRAFRDRRYDYKILTKKAKKAKSKAAEEGDAVGAKEAGDRAQVFDSLQLAHKCILNSFYGYVMRKGARWRSMEMAGIVTYTGAMLIKQARELVEQIGRPLELDTDGIWCILPSSFPENFTFTTKSGGKIRISYPCVMLNADVHENYTNHQYQDLQDPATRKYNTKSECSIFFEVDGPYRCMVLPSSTEEGKLLKKRYAVFNHDGSLAELKGFELKRRGELEVIKVFQSQVFEKFLRGDTLAQCYDAVGEVANQWLDVLYSKGGDVDDDELMELISQNKTMSQTLEDYGTAKGTSQTTARRLADFLGAEMVKDKGLNCRMILSNRPHGAPVTDRAVPTAIFSAEPAVKKHYLRKWLKDPAMEDFDIRSILDWDYYLDRLGNCIRKIITIPAAMQKVENPCPRVKHPDWLRKIVREINDGYRQMKIDQLFKAAPPGTPQLTNAGASTKAKPTPAPAATDIEDMAGXXXXXAAGRSILGARARVTKRIGNGAAAAGALAPDGSPGKENADVGXXXXXXXXXXXXXXPIRREDDFQGWLAQQKAGWKKRREQNRLKRRTEARSQAMASFRGSGSAGARGGTAGLGAAGAAEKRR-------RTAAMGKS-GLQGYLTSAQDALLRGFWQVVEIRPSDNPGSFTVFAMTGPGE-LQVIPITIPRVFYVNCLE-DKGPAGSSTMG-RRVARTLPNGRGSP-FLYEVAMDEAKFQRNEKQVEDFLHHAGIEGVYELGTPLVYRAMLHLGSVARVVQGRAKALKGVKSGSGQAPGFELEDLELVTAAAHPYLHP-----------SVATFKHIYLYHSSSGKRGMVGLFYVQGTNEDIEPEPTAEGGAEDVGGEGGLGPGERPVIAKAVVWLVNPFSR---RDGEQRPPFARIFEKFTNN----PDSSCTFKTSSVSSADEAWAAADASLASYSRQRN---GPTLVLSQGLV-QGQELRRRLPALSELPVTHIPGNAEDERYPALGWQAFAAQRMVQRFFIAPRWLADRLLCARYAHLPLANIGADAPCAMADAFFGRLLRHNRHVLWASETSRPDLGGTEGDDN--DIWAEDL----------------------ASPTILQPGAYRNICIELEIHGLAVGSVIASSQLEALEGSQGVTALTVNNDNEGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQAVASAFRILKVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNATLHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQLEPKMFWEQLLFLDDQDYGGIQVQGGFKEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKRDNDLKPXXXXXXEDAQVSGRGVSGADGTVAEQKMPSIVSHWNLAEYLPASVRQVFLILVSTFLLKPHE-KARQLRLDHERMVASLSNQLTQSSQPNGPASPGTDRGESXXXXXXXXXVPASQGGMDAALAVQAALRKEEANESQSMADFLKHLVTRTLTDKVLETVPAIKKYSGSGPDAFPNKPGAARELTNPALEFIKSLCHVLSLDPTVTDEVETLRKQALAEVGVRQFSAEGQYSDPCVSFVLPDVICSFCNLCRDLDLCRDSRLM--------DEDEDER----------WQCIQCNHPYERSSIELSLVEAVQRRSVRYQLQDMKCAKCGAVATRSMSLTCPCSGPLVAEESQQE-FHKTMKLLRQLAIFHNFGWLLETVENI 2503
            +GDELDK+FG+    E                      RLGW+ N+   ++PD     NE S L++YFLEQ G  FK  V Y+PYFYV  +  R ++    C+R+ FE    +   V +EDLDM +HL+G+   ++KLSFH+VADLM VK+ L P +  N+ R +A+ AY M  + REQ   D L                   S L++ REYDVPY +R +IDLDIRVGAWY V    T      +V  QK+MVDKAEP V A+DIECTKAPLKFP+ ++DQ++MISYM+D QGYLI++RE VSE++++FEYTPK ++PGPF +FNE NEE +++RFF HV E +P I VTYNGDFFDWPF++ RA+ +G++M  EIGV L + +GEYRGRC  HMDA  WVKRDSYLPQGS GLKAVTKYKLGYDPVEVD EDMV  A + P +MA+YSVSDAVAT+YLY  YVH+FVFSLCTIIP+G +DVLRKGSGTLCEALLMVEA+ GNI+CPNK S     KF+  HLVE+ETY+GGHVECLE+GVFR+D    F+LVPSA Q LIDNVDRDL F++E+E  V  D V+NYD V+ +I++QL  LR+ P R+E P IYHLDVAAMYPNIIL+NRLQP A+VN+A CA+C FN E           +C+R + W WRGD  PA+R EY S+K QL YEN                 D K        PF +L    Q   ++ R K+Y   VYKKTKIT+E++R  TVCMRENPFYVNTVRAFRDRRY+YK LTK  KK  S+AAE  D +   +A ++  V+DSLQLAHKCILNSFYGYVMRKGARW SMEMAGIVT+TG+ +I  ARELVEQIGRPLELDTDGIW ILP SFPE F+F  K G    ISYPCVMLNADVH  +TN QY +L DP T KY T  ECSI FEVDGPY+ MVLP+STEEGKLLKKRYAVFN D SLAELKGFELKRRGEL++IK FQSQVF+ FL GDTL +CY +V + AN WLDV+ ++G  ++DDELM LI++NK+MS  L+DY   K TS TTARRL +FLG EM+KDKGL C+MI++ RP+G  VT+RA+PTAIF+ E AVK+H+L+KWLKDP ++DFDIRSILDWDYY  R  + ++KII++PAA Q V NP PR++ PDW+RK VRE N  ++Q  + Q FK A  G   + N     +          D+ED+          ++       ++++ GN   A G   P  S  + + D                    E  F  WL  +K  WK     + L +R   R + M SF          G  GL A G    +R         AA G   G++ ++ +A  AL   +WQ+VEI+  +  G FT + +T   + LQ I + +PR+FY+ C E D     +   G RRV+R +P+    P  + EV + E ++Q+  K++   L    ++GVYEL    + RA+  LG VA+VV           S S Q   +EL DL+  + A  PYL             S +  + + LY + S KR ++GL  ++    D E      GGA   G               A VW V+PFS    R  + +  F ++ ++        P  SC FKT  V +  +A++  +A+LA  +  +     PT+V++Q  +   + LR ++  L   P+  +P N ED  +PAL W+    ++M+ R F   ++ +D L CARYAHLP+ N   D   A+ D  + R+L  ++H+ W S +S PDLGG E +     ++++ L                       S  + + G++ ++C+ELE+ GLAV +++ +SQ+ ++EG      + ++ D  G+                                  ++   AFR+L+V+VTT   +     N  AD +L  FYRW+C+  SL  +  LH +V       F +L+AE RRLG+ +V+A F K+II T K  V+ A+ Y +F++ TV S +LF  +   PK ++  L F+D ++YG I ++                                                                                                                 + A    R  + AD   +E K   IVSHWN+A YLP  V + FLILV  F+ +  E + R+LR   +   A L        QP                             +D           E+  +   +A + + LV+   ++K+L  VP I  Y+    D+FP   G+   L +P+LE +K +  V  L+P+V  +V  +R+  L  + + +FS E Q+ +P +SF + DVIC  CNLCR +DLCRD +L         ++DE +           W C +C   Y++++ E+ LVE VQ +SV YQLQD+ C KC   A   M   CPCSG        +E   +T++LL+++A  HNF WLLETV+ +
Sbjct:   54 KGDELDKKFGFHLLPELLEQEEEARKEHDISMDDQDHARLGWLLNLRACTLPDPVEGKNEVSALELYFLEQAGKAFKTRVIYQPYFYVRPETNRSLEVIAYCLRK-FEGLVAKAERVFREDLDMADHLNGRKALYVKLSFHTVADLMAVKKDLAPLVARNQERLQARQAYEMQGQTREQVTEDELMFGGDGLDGVKGAGTDESESSLLELREYDVPYPMRVAIDLDIRVGAWYHVNYDPTAPYAPSDVKRQKDMVDKAEPVVCAFDIECTKAPLKFPDAQVDQIYMISYMIDRQGYLIVNREFVSEEIQDFEYTPKEAYPGPFIVFNEENEEQLLKRFFEHVVEEQPHIFVTYNGDFFDWPFIETRAQIHGINMGHEIGVSLDERSGEYRGRCSVHMDAFCWVKRDSYLPQGSQGLKAVTKYKLGYDPVEVDAEDMVNLARDEPLKMASYSVSDAVATFYLYDKYVHLFVFSLCTIIPLGSEDVLRKGSGTLCEALLMVEAFGGNIICPNKQSSAREEKFYKNHLVETETYVGGHVECLESGVFRADFLYDFQLVPSAFQQLIDNVDRDLRFSLEIEMNVPIDRVTNYDAVKKDIVDQLVGLRERPNRKETPLIYHLDVAAMYPNIILTNRLQPCAIVNEADCAACAFNTECGRAAIKQGDASCQREMDWVWRGDFYPANRPEYYSIKTQLEYENFPVDKGPPGAANASTRNDPKGSTPGSSVPFAQLPTEKQELLLKQRFKSYCSSVYKKTKITEEQKRSATVCMRENPFYVNTVRAFRDRRYEYKGLTKVWKKRVSQAAEAKDLLAKIDAQNKCLVYDSLQLAHKCILNSFYGYVMRKGARWHSMEMAGIVTHTGSNIITGARELVEQIGRPLELDTDGIWAILPKSFPETFSFKLKDGSSRSISYPCVMLNADVHAKFTNPQYHEL-DPETGKYETHQECSILFEVDGPYKAMVLPASTEEGKLLKKRYAVFNFDHSLAELKGFELKRRGELQIIKAFQSQVFDCFLDGDTLEECYSSVAKCANHWLDVIDTRGKSMEDDELMALITENKSMSGRLDDYEGQKSTSITTARRLGEFLGEEMIKDKGLTCKMIIAARPYGEKVTERAIPTAIFATEDAVKRHFLKKWLKDPHLQDFDIRSILDWDYYRGRFASTVQKIISLPAAFQNVRNPVPRIELPDWMRKQVREKNAKHQQGNMKQFFKTADKG---VENGDYIAEL--------IDMEDLVKGNPSSHRSKN-----PSLSRKRGNKEDAGGDTGPTISAEEIDNDADSDGNMPATKVKLG-----EVPFTDWLKNRKKKWK-----SMLGKRRREREERMRSF----------GEFGLMAGGRGSAKRFQSSSSSALAAKGNGVGMENFVFNAGSALQSEYWQIVEIQ-QEQGGLFTCWVLTAHSQMLQRITLKMPRIFYLTCDEADTDNLLTLLPGSRRVSRIVPHYSSKPQSVLEVTLSERQYQQYHKEINQILGDPHVKGVYELQVSALTRAVCALGCVAKVVNPHL-------SSSNQE--YELSDLQFQSTAQAPYLMDTDLPTGGTLSLSNSLMRRVLLYFAQSKKRAILGLVVLK----DEEDGGDTSGGAISEG--------------DANVWYVDPFSNVKSRGSDLKQFFEQLLQESQEEGGDAPIQSCAFKTHVVKTVQDAFSGVNATLARLTSNKQLAPKPTIVIAQTCIPSSKRLREKMQGLHSFPIATLPWNEEDTLFPALTWRKLMGEKMLTRCFEMTQFYSDVLDCARYAHLPVGNFTGDHSIAILDTLYSRILTKHKHMWWHSPSSLPDLGGKEQEQQFQQLFSQSLFGSAQATQMKNDKGSATQHRVDTSVVVCEKGSFVDVCVELELDGLAVNAILVASQIHSIEGLGVAHQMEIDFDGNGDDESNTKAEGTSAAMAAEGDAE-------------ESCHEAFRLLRVMVTTLFKDFLSSRNKFADHVLQQFYRWLCSPGSLCFDPALHAMVKRLMEKLFLQLLAEFRRLGSHIVYADFSKIIICTKKKSVRDAQTYLQFILQTVLSNELFQVMNFTPKKYFSNLFFMDAENYGCILLK-----------------------------------------------------------------------------------------------------------------DVAATEERIENEADTEDSEVKPLEIVSHWNIANYLPRGVDEYFLILVGQFIRRRAEFQLRELRKGKD---AKLDIAEDADEQP-----------------------------LD-----------EDEKQLSPLAKYSQRLVSSYFSEKMLRLVPEILTYNMDR-DSFPVFAGSHLPLESPSLELVKFVSSVFLLEPSVEAQVGKMRRTLLKLLRMSEFSDESQFRNPSLSFTVQDVICLCCNLCRSVDLCRDPQLFKCSVASSNEDDEGQENAEEAEETLSWHCPRCFALYDKTAFEMRLVEMVQAQSVAYQLQDLYCRKCHLPAEHKMREYCPCSGTYALMPGVRESLLETLRLLKRIAQQHNFEWLLETVQRL 2401          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16166.1 vs. uniprot
Match: A0A2R5GVQ4_9STRA (DNA-directed DNA polymerase n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GVQ4_9STRA)

HSP 1 Score: 1764 bits (4568), Expect = 0.000e+0
Identity = 1106/2590 (42.70%), Postives = 1491/2590 (57.57%), Query Frame = 0

Query:   74 ALREQGDELDKRFGYDRYSEGPPRLGWIFNMLPTSIPDASGN-----EKSGLDMYFLEQDGGTFKATVFYKPYFYVLVKEERYIQDCIQCIRRRFEDSAVEVTVVDKEDLDMPNHLSGKLRRFLKLSFHSVADLMEVKRALMPTIQANKTRAEAQDAYMH----AEAREQQPSDFLSVLVDAREYDVPYYVRCSIDLDIRVGAWYMVTPQ------EEGVEVAWQK-EMVDKAEPKVLAYDIECTKAPLKFPNVEIDQVFMISYMVDGQGYLIISREVVSEDVENFEYTPKPSFPGPFHIFNEPNEEAVIRRFFNHVAELRPQIIVTYNGDFFDWPFVDERAKGYGLDMEKEIGVGLQANG-EYRGRCVAHMDAIYWVKRDSYLPQGSHGLKAVTKYKLGYDPVEVDPEDMVRYASERPTEMAAYSVSDAVATYYLYQTYVHMFVFSLCTIIPMGPDDVLRKGSGTLCEALLMVEAYRGNIVCPNKHSEPLTKFHD-GHLVESETYIGGHVECLETGVFRSDLPEKFRLVPSALQALIDNVDRDLTFAIEVESGVQRDTVSNYDEVRSEIIEQLEMLRDSPLREEEPFIYHLDVAAMYPNIILSNRLQPGAVVNKAMCASCDFNQESNN-CKRSLTWKWRGDMTPASRTEYQSVKMQLTYENVDGKPFVELDEADQATNVRARLKNYAHKVYKKTKITKEEERVDTVCMRENPFYVNTVRAFRDRRYDYKILTKKAKKAKS---KAAEEG----DAVGAKEAGDRAQVFDSLQLAHKCILNSFYGYVMRKGARWRSMEMAGIVTYTGAMLIKQARELVEQIGRPLELDTDGIWCILPSSFPENFTFTTKSGGKIRISYPCVMLNADVHENYTNHQYQDLQDPATRKYNTKSECSIFFEVDGPYRCMVLPSSTEEGKLLKKRYAVFNHDGSLAELKGFELKRRGELEVIKVFQSQVFEKFLRGDTLAQCYDAVGEVANQWLDVLYSKGGDVDDDELMELISQNKTMSQTLEDYGTAKGTSQTTARRLADFLGAEMVKDKGLNCRMILSNRPHGAPVTDRAVPTAIFSAEPAVKKHYLRKWLKDPAMEDFDIRSILDWDYYLDRLGNCIRKIITIPAAMQKVENPCPRVKHPDWLRKIVREINDGYRQMKIDQLFKAAPPGTPQLTNAGASTKAKPTPAPAATDIEDMAGXXXXXAAGRSILGARARVTKRIGNGAAAAGALAPDGSPGKENADVGXXXXXXXXXXXXXXPIRRE----DDFQGWLAQQKAGWKKRREQNRLKRRTEARSQAMASFRGSGSAGARGGTAGLGAAGAAEKRRRTAAMGKSGLQGYLTSAQDALLRGFWQVVEIRPSDNPGSFTVFAMT-GPGELQVIPITIPRVFYVNCLEDKGP--AGSSTMGRRVAR-TLPNGRGSPFLYEVAMDEAKFQRNEKQVEDFLHHAGIEGVYELGTPLVYRAMLHLGSVARVVQGRAKALKGVKSGSGQAPGFELEDLELVTAAAHPYLHPSVATFKHIYLYHSSSGKRGMVGLFYVQGTNEDIEP----EPTAEGGAEDVGGEGGLGPGERPVIAKAVVWLVNPFSRRDGEQRPPFARIFEKFT-------------NNPDSSCTFKTSSVSSADEAWAAADASLASYSRQRNGPTLVLSQGLVQGQELRRRLPALSELPVTHIPGNAEDERYPALGWQAFAAQRMVQRFFIAPRWLADRLLCARYAHLPLANIGADAPCAMADAFFGRLLRHNRHVLWASETSRPDLGGTEGDDND---IWAEDLASPTILQPGAYRNICIELEIHGLAVGSVIASSQLEALEGSQGVTALTVNNDNEGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQAVASAFRILKVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNATLHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQLEPKMFWEQLLFLDDQDYGGIQVQGGFKEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKRDND----------------------------------------------------------LKPXXXXXXEDAQVSGRGVSGADGTVAEQK-------------------------------------------MPSIVSHWNLAEYLPASVRQVFLILVSTFLLKPHE-KARQLRLDHERMVASLSNQLTQSSQPNGPASPGTDRGESXXXXXXXXXVPASQGGMDAALAVQAALRKEEANESQSMADFLKHLVTRTLTDKVLETVPAIKKYSGSGPDAFPNKPGAARELTNPALEFIKSLCHVLSLDPTVTDEVETLRKQALAEVGVRQFSAEGQYSDPCVSFVLPDVICSFCNLCRDLDLCRDSRLMD-------EDEDERWQCIQCNHPYERSSIELSLVEAVQRRSVRYQLQDMKCAKCGAVATRSMSLTCPCSGPLVAEESQQEFHKTMKLLRQLAIFHNFGWLLETV 2500
            ALREQ + +D+ +G+D ++EGP R+GW+ NMLP ++           E S L++YFL+QDG TFKA + ++PYFYVLVKEE ++++    + RRF     EV +VD+EDLDM +HL+GK R  L+L F +V DLM V+  +   ++ NK+R++A DAY      A    + P D +  +VD REYDV Y  R SIDLD+ VG+WY V+ +       EG+ V  +K ++++KAEP++LA+DIECTK P KFP+   DQVFMISYM DGQGYLII+REVV+ED+E+FEYTPKP +PGPF +FNE +EE+ +R+FF H  ELRP + VTYNGDFFDWPF+++RA  Y +D+  E+GVG    G EYRGRC  HMD +YWVKRDSYLP GS GLKAVTK KLGYDPVEVDPEDMVR+A ERP  MA+YSVSDAVATYYL+  Y+++F+FSLCTIIP+ PDDVLRKGSGTLCE LLMVEA RGNI+CPNK  +P  KF D G L+E +TYIGGHVECLETGV+R DLPEKFRL P A   LI N+DRDLTFAIE+E+G+ R  V NY  VRSEI+E LEMLRD+P+R E P IYHLDVAAMYPNIIL+NRLQP A+V++  CASC FN   +  CKR + W WRG+  PA R+EYQ++K QL YE VDG P+ EL   D+   +R R+K Y+  VY++ K T+  ++  T+C REN FYV+T++AFRDRRY+YK LTKK  K KS   K  E G    D +   EA ++  ++DSLQLAHKCILNSFYGYVMRKGARW SMEMAGIVT+TG  +IKQAR+LVE+IGRPLELDTDGIWCILP+SFPENF   T +G KI ISYPCVMLNADVH ++TNHQYQ L DP   +Y+T+SECSI+FEVDGPYRCMVLP+S EEGKLLKKRYAVFNHDG+LAELKGFELKRRGEL++IK+FQSQVFEKFL G +L +CY AV E+AN WLDVL+++G D+ D+EL+ELIS+N++MS+ +E+YG  K  + TTARRL++  GAEM++D GLNC+++++ +P G   T RA+P  IFS     K+  LR+WL+D ++ DFD+RSI+DWDYY+DRLG  ++KIITIPAA+Q V NP PR++HP WL + VRE N  ++Q K+  LF+A+           A +    T APA  DIEDM G       G S  GA+ +            G  A  G          XXXXXXXXXXXXXX    E     DF GWLA +K  W++R+E   L +  +   QA+ S                GA G A K        ++GLQG++   +     G+ QV+E+RP D+ G+F VF +     E++ + + + R F++N  E   P  A      R  AR  LP+      LY++   E   +++ K V D L    + GVYEL T    R +  LG +  V Q   + L G   G+   P F + ++  V  A  PYL  S AT +  +L+     +R  V +F V  T+ ++          +  A+ V     L P E  + A    W+      R G +RP   +    F              N   S    + ++V++    W   +  L   +R+ +G    +    +  +EL  +   L   P   +    +    P+L WQ +     V+++F    WL +R+ CA YA +P+A++G D   AM D  F R L  N HV WAS    PDLGG E D  D   +  E+L  P I  PG YR+ C    +  LAV +++ + ++  +E ++G  AL      + +                                       AF IL+ LV     E       +  +LL +FYRW+C+ +SL+++  L R V       F +L+AELRRLGA +V A+FQ++II T K D  +A  Y + V++TV S+ LF  ++LE    W+ L+F+DD ++G +Q    + E                                        XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX           K D +                                                          L         D++   RG +  +  + E++                                             +++  W++A+YLP  +++ F + V  FLLKP E +   L    E++ A +  +  ++  P+   +   D   S                    L   A LR  EA       DF+  L+   +  ++L  +P I   SG G ++FP   G+     +PALEF+K+LC VLSL+  V  +V+T +KQ L  + VR+F+ E Q+ +P ++  + DV+CS CN   D DLCRD+ L+        +   + W C +C   ++ +SIE  L+  V+   + ++LQD +C KCG V    +S +C CSG  V   S ++    ++ L  +A F+ F WLLE+V
Sbjct:   41 ALREQSERVDEMYGFDDFTEGPARVGWLLNMLPCTVNFVDPTLEKPVELSALELYFLQQDGNTFKAKILHRPYFYVLVKEE-FLKETKAMLERRFASDVSEVAMVDREDLDMADHLAGKHRVLLQLFFRNVNDLMNVRLEVQEAVRVNKSRSKAADAYNFTGHDATGSTELPDDLMEAIVDLREYDVQYQTRVSIDLDVNVGSWYEVSTRFSDGTGAEGLGVDLKKLDLLEKAEPRILAFDIECTKQPHKFPDAAFDQVFMISYMFDGQGYLIINREVVTEDIEDFEYTPKPEYPGPFEVFNEADEESTLRKFFEHCRELRPNVWVTYNGDFFDWPFIEKRAATYDMDLRTEVGVGPNKEGTEYRGRCSVHMDCLYWVKRDSYLPAGSRGLKAVTKAKLGYDPVEVDPEDMVRFARERPHHMASYSVSDAVATYYLFDKYINLFIFSLCTIIPLPPDDVLRKGSGTLCEMLLMVEARRGNILCPNKQVDPAQKFTDKGQLLEQDTYIGGHVECLETGVYRDDLPEKFRLDPLAFDELIANIDRDLTFAIEIEAGIPRREVVNYAAVRSEIVEALEMLRDTPVRLETPTIYHLDVAAMYPNIILTNRLQPSAIVDETDCASCSFNTGPDAACKRPMDWVWRGEFYPAKRSEYQAIKTQLEYEQVDGVPYHELPRGDRNRMLRQRVKTYSQTVYRRVKDTEITKKSATICQRENSFYVDTIKAFRDRRYEYKRLTKKWAKEKSTVEKRLETGSASADPLALDEARNKVLLYDSLQLAHKCILNSFYGYVMRKGARWHSMEMAGIVTHTGGNIIKQARKLVERIGRPLELDTDGIWCILPASFPENFALKTGAGRKIPISYPCVMLNADVHAHFTNHQYQSLVDPKHLQYDTRSECSIYFEVDGPYRCMVLPASQEEGKLLKKRYAVFNHDGTLAELKGFELKRRGELKIIKIFQSQVFEKFLLGGSLPECYAAVAEIANYWLDVLFTRGKDLSDEELIELISENRSMSRKVEEYGDRKSVAMTTARRLSEIFGAEMIRDAGLNCKLLIARKPVGEMTTARAIPAVIFSLGEDAKRQALRRWLRDNSITDFDVRSIVDWDYYIDRLGKAVQKIITIPAALQNVANPVPRIEHPAWLLRDVREKNGRFKQSKLSALFQAS-----------AKSSTSKTSAPAVADIEDMLGGGRD---GSSSSGAKPK------------GKSARSGENXXXXXXXXXXXXXXXXXXXXXXXANAEIGDPQDFDGWLAARKTMWRRRQE---LYKAQQKEQQALTSS---------------GAGGPAPKFSL-----RNGLQGFVQRREALSDFGYLQVLEVRPVDSAGTFRVFVLPENNDEVRSMYMKVNRKFFLNSREPCDPKEAPPWLALRDAARWKLPHAHAVEHLYQLEAPEKYMRKHAKTVADILEQPNVAGVYELQTSPESRILSELGCMISVNQ---RNLSG--DGTASRP-FAMAEVRKVARAGAPYLLGSGATLQRFFLFVGGRPQRRAVVIFQVSQTSNELAKIAWSAMRGDKAADQVDTLLSLAPQEVRIHA----WIEG--HERSGTERPSLRKAMRSFLDDCSKESRTAHAENLRSSVAHAEVTTVATEGALWHKVNEHLVKMARELSGAVTAVVHSALSPRELLAKSMQLGHFPCIWMTAREDTRALPSLQWQKYLFDEAVRQYFAHSLWLFERMRCAHYAQVPVAHLGDDYAVAMMDVMFMRNLSRNSHVSWASPACTPDLGGMESDSADSALLAVEEL--PVIASPGTYRSYCAVFSLKHLAVNTMLHADEVHDIECAEGSLALE-----QHSMGALAPDGGTQRLDAEGAVVHAKVHAATGVLGSAAECLGAFNILRGLVRRMFREALQTNEDYCWALLTNFYRWLCSPKSLMYDPALLRFVQQLMSKVFLQLVAELRRLGAKIVSANFQRIIICTGKRDAASASAYVEHVINTVQSKSLFEDIELELADTWKTLIFMDDANFGAVQDNPTYNEELEAYEKRQARRKQIESERQARRDGTLVDAPRETSNQKVSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAEVSPVVRKTAKADKESXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVAAADGYVENEDSASDSDLDDEEFDGNLTRLKRRVMTDSRRPKRGRNKREDRIIEREDELNEAEDMDDFVVDDDGDSFYGAAASRDADEEALLEAARPPFKHTLILQWDVAQYLPEELQRYFELYVGAFLLKPVEFRESWLSAQKEKLRAEMHVEREETKDPSRDIAGKADGSNSDE------------------LDELALLRVNEAELEVEEKDFVTKLLKNEVVAELLRLIPRIT-MSGLGAESFPKLAGSHLVYKDPALEFVKTLCVVLSLEGQVHHQVQTAKKQLLRLLHVREFAPEAQFHNPSLTIHMRDVVCSHCNAAVDFDLCRDTALVQGQLAAGPDAGTQPWHCTECAETFDMASIEHRLIRRVEDLVLAFELQDFECIKCGQVRADVLSASCTCSGDWVLTISPKDQETALRPLHNVAKFYGFEWLLESV 2542          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16166.1 vs. uniprot
Match: A0A662YIT8_9STRA (DNA polymerase epsilon catalytic subunit n=1 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662YIT8_9STRA)

HSP 1 Score: 1759 bits (4556), Expect = 0.000e+0
Identity = 1099/2627 (41.83%), Postives = 1483/2627 (56.45%), Query Frame = 0

Query:   53 ADSSQAARLSG----GGRKESAVKAALREQGDELDKRFGYDRY-------------------SEGPPRLGWIFNMLPTSIPD-ASGN-EKSGLDMYFLEQDGGTFKATVFYKPYFYVLVKEERYIQDCIQCIRRRFEDSAVEVTVVDKEDLDMPNHLSGKLRRFLKLSFHSVADLMEVKRALMPTIQANKTRAEAQDAY-MHAEAREQQPSDFL-------------------SVLVDAREYDVPYYVRCSIDLDIRVGAWYMVT-----PQEEGVEVAWQKEMVDKAEPKVLAYDIECTKAPLKFPNVEIDQVFMISYMVDGQGYLIISREVVSEDVENFEYTPKPSFPGPFHIFNEPNEEAVIRRFFNHVAELRPQIIVTYNGDFFDWPFVDERAKGYGLDMEKEIGVGLQA-NGEYRGRCVAHMDAIYWVKRDSYLPQGSHGLKAVTKYKLGYDPVEVDPEDMVRYASERPTEMAAYSVSDAVATYYLYQTYVHMFVFSLCTIIPMGPDDVLRKGSGTLCEALLMVEAYRGNIVCPNKHSEPLTK-FHDGHLVESETYIGGHVECLETGVFRSDLPEKFRLVPSALQALIDNVDRDLTFAIEVESGVQRDTVSNYDEVRSEIIEQLEMLRDSPLREEEPFIYHLDVAAMYPNIILSNRLQPGAVVNKAMCASCDFN---------QESNNCKRSLTWKWRGDMTPASRTEYQSVKMQLTYEN---------------------VDGKPFVELDEADQATNVRARLKNYAHKVYKKTKITKEEERVDTVCMRENPFYVNTVRAFRDRRYDYKILTKKAKKAKSKAAEEGDAVGAKEAGDRAQVFDSLQLAHKCILNSFYGYVMRKGARWRSMEMAGIVTYTGAMLIKQARELVEQIGRPLELDTDGIWCILPSSFPENFTFTTKSGGKIRISYPCVMLNADVHENYTNHQYQDLQDPATRKYNTKSECSIFFEVDGPYRCMVLPSSTEEGKLLKKRYAVFNHDGSLAELKGFELKRRGELEVIKVFQSQVFEKFLRGDTLAQCYDAVGEVANQWLDVLYSKGGDVDDDELMELISQNKTMSQTLEDYGTAKGTSQTTARRLADFLGAEMVKDKGLNCRMILSNRPHGAPVTDRAVPTAIFSAEPAVKKHYLRKWLKDPAMEDFDIRSILDWDYYLDRLGNCIRKIITIPAAMQKVENPCPRVKHPDWLRKIVREINDGYRQMKIDQLFKAAPPG------TPQLTNAGASTKAKPTPAPAATDIEDMAGXXXXXAAGRSILGARARVTKRIGNGAAAAGALAPDGSPGKENADVGXXXXXXXXXXXXXXPIRR----EDDFQGWLAQQKAGWKKRREQNRLKRRTEARSQAMASFRGSGSAGARGGTAGLGAAGAAEKRRRTAAMGKS---GLQGYLTSAQDALLRGFWQVVEIRPSDNPGSFTVFAMTGPGE-LQVIPITIPRVFYVNCLEDKG-------PAGSSTMGRRVARTLPNGRGSPF-LYEVAMDEAKFQRNEKQVEDFLHHAGIEGVYELGTPLVYRAMLHLGSVARVVQGRAKALKGVKSGSGQAPGFELEDLELVTAAAHPYL--------------HPSVATFKHIYLYHSSSGKRGMVGLFYVQGTNEDIEPEPTAEGGAEDVGGEGGLGPGERPVIAKAVVWLVNPFSR---RDGEQRPPFARIFEKFTNNPDS----SCTFKTSSVSSADEAWAAADASLASYSRQRN---GPTLVLSQGLVQGQE-LRRRLPALSELPVTHIPGNAEDERYPALGWQAFAAQRMVQRFFIAPRWLADRLLCARYAHLPLANIGADAPCAMADAFFGRLLRHNRHVLWASETSRPDLGGTEGDDN--DIWAEDL-----------------ASPTILQPGAYRNICIELEIHGLAVGSVIASSQLEALEGSQGVTALTVNNDNEGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQAVASAFRILKVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNATLHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQLEPKMFWEQLLFLDDQDYGGIQVQGGFKEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKRDNDLKPXXXXXXEDAQVSGRGVSGADGTVAEQKMPSIVSHWNLAEYLPASVRQVFLILVSTFLLKPHEKARQLRLDHERMVASLSNQLTQSSQPNGPASPGTDRGESXXXXXXXXXVPASQGGMDAALAVQAALRKEEANESQS----MADFLKHLVTRTLTDKVLETVPAIKKYSGSGPDAFPNKPGAARELTNPALEFIKSLCHVLSLDPTVTDEVETLRKQALAEVGVRQFSAEGQYSDPCVSFVLPDVICSFCNLCRDLDLCRDSRLM--------DEDEDER---------WQCIQCNHPYERSSIELSLVEAVQRRSVRYQLQDMKCAKCGAVATRSMSLTCPCSGPLVAEESQQE-FHKTMKLLRQLAIFHNFGWLLETVENILVADGL 2509
            A +SQ AR +     GGR  +  K    ++GDELDK+FG+                       E   RLGW+ N+   ++PD A G  E SGL++YFLEQDG  FK  V Y+PYFYV  +  R  Q+ I    R+FE    +   V +EDLDM +HL+GK   ++KLSF +V+DLM VK+ L P +  N+ R +A+ AY M  +AREQ   D L                   S L++ REYDVPY +R +IDLDIRVGAWY VT     P     +V  QK+MVDKAEP V A+DIECTKAPLKFP+ ++DQ++MISYM+D QGYLI++RE VSE++ +FEYTPK S+PGPF +FNEPNEE +I+RFF HV + +P I VTYNGDFFDWPF++ RA+ +G++M  EIG+ L   +GEYRGR   H+DA  WVKRDSYLPQGS GLKAVTKYKLGYDPVEVD EDMV  A + P +MA+YSVSDAVAT+YLY  YVH+FVFSLCTIIP+G +DVLRKGSGTLCEALLMVEA+ GNI+CPNK S    + F+ GHLVE+ETY+GGHVECLE+GVFR+D    F+LVPSA Q LIDN+DRDL F++E+E  V  D V+NYDEV+ +I+++L  LR+ P R+E+P IYHLDVAAMYPNIIL+NRLQP A+VN+A CA+C FN         Q+  +C+R + W WRGD  PA+R EY S+K Q+ YEN                     V   PF +L    Q   ++ R K+Y   VYKKTKIT+E++R  TVCMRENPFYVNTVRAFRDRRY+YK LTK  KK  S+AAE  D +   EA ++  V+DSLQLAHKCILNSFYGYVMRKGARW SMEMAGIVT+TG+ +I  ARELVEQIGRPLELDTDGIW ILP+SFPE F+F  K G    ISYPCVMLNADVH  +TN QY +L DPAT KY T  ECSI FEVDGPY+ MVLP+STEEGKLLKKRYAVFN D SLAELKGFELKRRGEL++IK FQSQVFE FL GD L +CY +V + AN+WLDV+ +KG  +DD+ELM LI++NK+MS  LE+Y   K TS TTA+RL +FLG +M+KDKGL C+MI++ RP+G  VT+RA+PTAIF+ E AVK+H+L+KWLKDP ++DFDIR+ILDWDYY  R  + ++KII++PAA Q V+NP PR++ PDW+RK VRE N  ++Q  + Q FK A  G        QL +     K KP+                  ++G+  L +                + + D     ++ DV               P+ +    E  F  WL  +K  WK       L +R   R + + SF G    G R G  G+ ++   +    +A   +    G++ ++ +   AL R +WQ+VEI+  +  G FT + +T   + LQ I + +PR+FY++C E          P GS    RRV R +P+    P  + EV + E ++Q+  K++   L    ++GVYEL    + RA+  LG VA+VV           S + Q   +EL DL+  + A  PYL               PS +  + + LY +   KR ++ L         +  E   +G  E  G +G            A VW V+PFS    R  + +  F ++ ++      +    SC FKT  V +A + ++  +A+LA  +  +     PT+V++Q  +   + LR ++  L   P+  +P N ED  +PAL W+    ++M+ R F   ++  D L CARYAHLP+ N   D   A+ D  + R+L  ++H+ W S TS PDLGG E +     ++++ L                 +S  + + G++ ++C+ELE+ GLAV +++ +SQ+ ++EG  GV A     D EGN     XXXXXXXXX                    ++   AFR+L+V+VTT   +     N  AD +L  FYRW+ +  SL  +  L  +V       F +L+AELRRLG+ +V+A F K+I+ T K  V+ A+ Y +F++ TV S +LF  L   PK ++  L FLD ++YG I +Q    E                                                                                                            E+ +   R   G D T   +K   IVSHWN+A YLP  V + FL+LV  F+ +  E                  QL Q ++                             G DA L       +E  ++S+     +A + + LV    ++K+L  VP I  ++    D+FP   G+   L +PALE +K +  V  L+PTV  +V  +++  L  +   +F+ E Q+ +P +SF + DVIC  CNLCR +DLCRD +L         + DE+E          W C +C   Y++++ E+ LVE VQ +SV YQLQD+ C KC   A   M   CPCSG        +E   +T++LL+++A  H F WLLETV+ +    GL
Sbjct:   33 ARNSQGARANAQGARGGRGMTEAKQYAMQKGDELDKKFGFHLLPDLLEQEEEARKDQPGTEDGEDHARLGWLLNLRACTLPDPAEGKFEVSGLELYFLEQDGKAFKTRVIYQPYFYVRSEPNR-SQEVIAYCLRKFEGLVAKAERVFREDLDMADHLNGKKALYVKLSFATVSDLMTVKKELAPLVARNQERQQARQAYEMQGQAREQVTEDDLLFGGGAGDGGKSAGTDESESALLELREYDVPYTMRVAIDLDIRVGAWYHVTYDPTTPYAPS-DVTRQKDMVDKAEPVVCAFDIECTKAPLKFPDAQVDQIYMISYMIDRQGYLIVNREFVSEEINDFEYTPKESYPGPFIVFNEPNEEQLIKRFFEHVVDEQPHIFVTYNGDFFDWPFIETRAQIHGINMAHEIGISLDIRSGEYRGRSSVHLDAFCWVKRDSYLPQGSQGLKAVTKYKLGYDPVEVDAEDMVALARDEPLKMASYSVSDAVATFYLYDKYVHLFVFSLCTIIPLGSEDVLRKGSGTLCEALLMVEAFEGNIICPNKQSSAHEENFYKGHLVENETYVGGHVECLESGVFRADFLYDFQLVPSAFQQLIDNIDRDLRFSLEIEMNVPVDRVANYDEVKKDIVDRLVGLRERPNRKEKPLIYHLDVAAMYPNIILTNRLQPCAIVNEADCAACAFNTECGRAAIKQQDASCQREMEWVWRGDFYPATRPEYYSIKTQIEYENFPPEHVVAGADKARGANGNAPVPTVPFAQLHTDRQEQLLKQRFKSYCSSVYKKTKITEEQKRAATVCMRENPFYVNTVRAFRDRRYEYKGLTKTWKKKVSQAAEAKDLLAKIEAQNKCLVYDSLQLAHKCILNSFYGYVMRKGARWHSMEMAGIVTHTGSNIITGARELVEQIGRPLELDTDGIWAILPASFPETFSFKLKDGSSRSISYPCVMLNADVHAKFTNPQYHEL-DPATGKYKTHQECSILFEVDGPYKAMVLPASTEEGKLLKKRYAVFNFDHSLAELKGFELKRRGELQLIKAFQSQVFECFLDGDNLEECYASVAKCANRWLDVIDTKGKSMDDEELMALITENKSMSGKLEEYAGQKSTSITTAKRLGEFLGEDMIKDKGLTCKMIIAARPYGEKVTERAIPTAIFATEDAVKRHFLKKWLKDPHLQDFDIRTILDWDYYRVRFASTVQKIISLPAAFQNVKNPVPRIELPDWMRKQVREKNAKHQQGNMKQFFKTAAKGDDNGDYVAQLIDMEDMMKGKPS------------------SSGKGKLQSLKXXXXXXXXXXXXGSSESDDPIEEIDDGDV-----------TADEPLAKVKLGEVPFTDWLKNRKKKWK-----TLLGKRRRERDERLRSFGGGADFGMRNG--GMPSSKRFQSSTTSAVTVRGNGIGMENFVLNTASALQREYWQIVEIQ-QEQGGLFTCWVLTARSQMLQRITLKMPRLFYLSCDEADNDDLLALLPVGS----RRVLRIVPHFSSKPQPVLEVMLSERQYQQYHKEMSQILSDPRVKGVYELHVSALTRAVCALGCVAKVVNPH-------HSTTNQE--YELSDLQFQSTAQAPYLVDLQRPMLSGNAAPRPSNSLMRRVLLYFAQQKKRAIICLVV-------LHEEEGVDG--ESAGCDG-----------DANVWYVDPFSNVKSRGSDLKQFFEQLLQETQEEGATAAIQSCAFKTHVVKTAQDGFSGVNATLARLASNKQLAAKPTIVIAQTCISSSKRLREKMQGLHSFPIAMLPWNEEDTLFPALTWRKLMGEKMLTRCFEMTQYYTDVLDCARYAHLPVGNFTGDHSIAILDTLYSRILSKHKHLWWHSPTSLPDLGGKEQEQQYQQLFSQSLLGSAAGKSDEAVQHRVDSSVVVCEKGSFVDVCVELELDGLAVNAILVASQIHSIEGL-GV-AHQRELDFEGNGESNGXXXXXXXXXEGDAE---------------ESCHEAFRLLRVMVTTLFKDFLASRNKFADHVLQQFYRWLSSSSSLCFDPALQTMVKRLMEKLFLQLLAELRRLGSQIVYADFSKIILCTKKQSVRDAQTYLQFILQTVLSNELFQVLNFTPKKYFSHLFFLDAENYGCILLQSAEAE------------------------------------------------------------------------------------------------------------EEGEGGDRDTEG-DTT---EKPLEIVSHWNVANYLPKGVDEYFLLLVGQFIRRRAEF-----------------QLRQQAK-----------------------------GGDARLEAAEEAMEEPLDDSEKQLSPLAKYSQRLVASYFSEKLLRVVPEILTHNLDR-DSFPVFAGSHLPLEHPALELVKFVSAVFLLEPTVEAQVGKMKRTLLKLLRTSEFADEAQFRNPSLSFTVQDVICLSCNLCRAVDLCRDPQLFKGTASGAGEGDEEEAATEVEAKAAWHCPRCFALYDKTAFEMRLVELVQAQSVAYQLQDLYCRKCRLPAEHKMREYCPCSGTYALLPGAREALMETLRLLKRIAQQHAFEWLLETVQRLERQAGL 2410          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16166.1 vs. uniprot
Match: A0A3M6VP32_9STRA (DNA polymerase epsilon catalytic subunit n=2 Tax=Peronospora effusa TaxID=542832 RepID=A0A3M6VP32_9STRA)

HSP 1 Score: 1754 bits (4544), Expect = 0.000e+0
Identity = 1068/2615 (40.84%), Postives = 1449/2615 (55.41%), Query Frame = 0

Query:   53 ADSSQAARLSGG----GRKESAVKAALREQGDELDKRFGYDRY--------------------SEGPPRLGWIFNMLPTSIPDA--SGNEKSGLDMYFLEQDGGTFKATVFYKPYFYVLVKEERYIQDCIQCIRRRFEDSAVEVTVVDKEDLDMPNHLSGKLRRFLKLSFHSVADLMEVKRALMPTIQANKTRAEAQDAY-MHAEAREQQPSDFLSV------------------LVDAREYDVPYYVRCSIDLDIRVGAWYMV----TPQEEGVEVAWQKEMVDKAEPKVLAYDIECTKAPLKFPNVEIDQVFMISYMVDGQGYLIISREVVSEDVENFEYTPKPSFPGPFHIFNEPNEEAVIRRFFNHVAELRPQIIVTYNGDFFDWPFVDERAKGYGLDMEKEIGVGL-QANGEYRGRCVAHMDAIYWVKRDSYLPQGSHGLKAVTKYKLGYDPVEVDPEDMVRYASERPTEMAAYSVSDAVATYYLYQTYVHMFVFSLCTIIPMGPDDVLRKGSGTLCEALLMVEAYRGNIVCPNKH-SEPLTKFHDGHLVESETYIGGHVECLETGVFRSDLPEKFRLVPSALQALIDNVDRDLTFAIEVESGVQRDTVSNYDEVRSEIIEQLEMLRDSPLREEEPFIYHLDVAAMYPNIILSNRLQPGAVVNKAMCASCDFNQESN---------NCKRSLTWKWRGDMTPASRTEYQSVKMQLTYEN-----------------VDGK----PFVELDEADQATNVRARLKNYAHKVYKKTKITKEEERVDTVCMRENPFYVNTVRAFRDRRYDYKILTKKAKKAKSKAAEEGDAVGAKEAGDRAQVFDSLQLAHKCILNSFYGYVMRKGARWRSMEMAGIVTYTGAMLIKQARELVEQIGRPLELDTDGIWCILPSSFPENFTFTTKSGGKIRISYPCVMLNADVHENYTNHQYQDLQDPATRKYNTKSECSIFFEVDGPYRCMVLPSSTEEGKLLKKRYAVFNHDGSLAELKGFELKRRGELEVIKVFQSQVFEKFLRGDTLAQCYDAVGEVANQWLDVLYSKGGDVDDDELMELISQNKTMSQTLEDYGTAKGTSQTTARRLADFLGAEMVKDKGLNCRMILSNRPHGAPVTDRAVPTAIFSAEPAVKKHYLRKWLKDPAMEDFDIRSILDWDYYLDRLGNCIRKIITIPAAMQKVENPCPRVKHPDWLRKIVREINDGYRQMKIDQLFKAAPPGTPQLTNAGASTKAKPTPAPAATDIEDMAGXXXXXAAGRSILGARARVTKRIGNGAAAAGALAPDGSPGKENADVGXXXXXXXXXXXXXXPIRREDDFQGWLAQQKAGWKKRREQNRLKRRTEARSQAMASFRGSGSAGARGGTAGLGAAGAAEKRRRTAAMGKS----------GLQGYLTSAQDALLRGFWQVVEIRPSDNPGSFTVFAMTGPGE-LQVIPITIPRVFYVNCLEDKGPAGSSTM--GRRVARTLPNGRGSP-FLYEVAMDEAKFQRNEKQVEDFLHHAGIEGVYELGTPLVYRAMLHLGSVARVVQGRAKALKGVKSGSGQAPGFELEDLELVTAAAHPYL---HPSVAT-----------FKHIYLYHSSSGKRGMVGLFYVQGTNEDIEPEPTAEGGAEDVGGEGGLGPGERPVIAKAVVWLVNPFSR---RDGEQRPPFARIFEKFTNN----PDSSCTFKTSSVSSADEAWAAADASLASYSRQRN---GPTLVLSQGLV-QGQELRRRLPALSELPVTHIPGNAEDERYPALGWQAFAAQRMVQRFFIAPRWLADRLLCARYAHLPLANIGADAPCAMADAFFGRLLRHNRHVLWASETSRPDLGGTEGDDN--DIWAEDL---------------------ASPTILQPGAYRNICIELEIHGLAVGSVIASSQLEALEGSQGVTALTVNNDNEGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQAVASAFRILKVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNATLHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQLEPKMFWEQLLFLDDQDYGGIQVQGGFKEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKRDNDLKPXXXXXXEDAQVSGRGVSGADGTVAEQKMPSIVSHWNLAEYLPASVRQVFLILVSTFLLKPHEKARQLRLDHERMVASLSNQLTQSSQPNGPASPGT--DRGESXXXXXXXXXVPASQGGMDAALAVQAALRKEEANESQSMADFLKHLVTRTLTDKVLETVPAIKKYSGSGPDAFPNKPGAARELTNPALEFIKSLCHVLSLDPTVTDEVETLRKQALAEVGVRQFSAEGQYSDPCVSFVLPDVICSFCNLCRDLDLCRDSRLM------------------DEDEDERWQCIQCNHPYERSSIELSLVEAVQRRSVRYQLQDMKCAKCGAVATRSMSLTCPCSGPLVAEESQQE-FHKTMKLLRQLAIFHNFGWLLETVENI 2503
            A +SQ AR +      GR  +  KA   ++GDELD++FGY                       +E   +LGW+ N+    +PD     NE SGL++YFLEQDG  FK  V Y+PYFY+  +  R  Q+ I    RRFE    +   + +EDLD  +HL+GK   ++KLSF +V DLM VKR L P I  N+ R + + AY +  ++RE+   + LS                   L++ REYDV Y +R +IDLDIRVGAWY+V    T      +V  QK+MVDKAEP V A+DIECTKAPLKFP+ ++DQ++MISYM+D QGYLI++R+ VSED+++FEYTPK ++PGPF +FNE NEE +++RFF HV E +P I VTYNGDFFDWPF++ RA+ YG++M +EIGV L +  GEYRGRC  HMDA  WVKRDSYLPQGS GLKAVTKYKLGYDPVEVDPEDMV  A + P +MA+YSVSDAVAT+YLY  YVH+FVFSLCTIIP+G +DVLRKGSGTLCEALLMVEA++GNI+CPNK  S    KF+ GHLVE+ETY+GGHVECLE+GVFR+D    F+LVP+A Q LIDN+DRDL F++EVE  V    V+NY+EVR +I+++L  LR+ P R+E+P IYHLDVAAMYPNIIL+NRLQP A+VN+A CA+C FN E           +C+R + W WRGD  PA+R EY S+K Q+ YEN                 ++G     PF +L    Q   ++ R K+Y   VYKKTK+T+E++R  TVCMRENPFYVNTVRAFRDRRYDYK LTK  KK  S+AA+  D +   +A ++  V+DSLQLAHKCILNSFYGYVMRKGARW SMEMAGIVT+TG+ +I  ARELVEQIGRPLELDTDGIW ILP SFPE F+F  K GG   ISYPCVMLNADV+  +TN QY +L DP T KY++  ECSI FEVDGPY+ MVLP+STEEGKLLKKRYAVFN D SLAELKGFELKRRGEL++IK FQSQVFE FL GD+L +CY +V + AN+WLDV+ ++G  ++D+ELM LI++NK+MS  L++Y   K TS TTARRL +FLG EM+KDKGL C+MI++ RP+G  VT+RA+PTAIF+ E AVK+H+L+KW+KDP ++DFDIRS+LDWDYY  R  + ++KII++PAA Q V NP PR++ PDW+RK VRE N  ++Q  + Q FK     T +  N     +          D+ED+         G+ I   R R  K            A DG          X              +     F  WL Q+K  WK       L +R   R   + SF G+          G G A +A    +    G +          G++ ++  A  AL R +WQ+VEI+  +  G FT + +T   + LQ I + +PRVFY+ C E       S +   RRV+R +P+    P  + EV + E ++Q  +K++   L    + GVYEL    + RA+   G VA+VV                   +EL DL+  + A  PYL     S+A                           ++GL  ++  ++DI    T  G A   G              +  VW V+P S    R  + +  F ++ ++        P  SC+FKT  V +A EA++  +A+LA  +  +     PT+V++Q  +   + LR ++  L   PV  +P N ED  +PAL W+    ++M+ R F   ++ +D L CARYAHLP+ N   D   A+ D  + R+L  ++H+ W S TS PDLGG E +     ++++ L                      S  + + G++ ++C+ELE+ GLA+ +++ +SQ+ ++EG      + ++ D  G                                   ++   AFR+L+++VTT   +     N  AD +L  FYRW+C+  SL  +  L  +V       F +L+AELRRLG+ +V+A F K+I+ TNK  V+ A+ Y  F++ TV S +LF  L   PK ++  LLFLD ++YG I ++                                                                                                        L P      +    +   V G+D     +K   IVSHWN+A YLP  V + FLILV  F+ +  E   + R  HER                G   P    D GE                              E+  +   +A + + LV+   ++K+L  VP I  Y+    D+FP   G+   + +PALE +K +  V  L+P+V  +V  +R+  L  + + +F  E Q+ +P +SF + DVIC  CNLCR +DLCRD +L                   D +    W C +C   Y+ ++ E+ LVE +Q +SV YQLQD+ C KC   A   M   CPCSG        +E   +T++LL ++A  HNF WLLETV+ +
Sbjct:   25 ARNSQGARANAARLRPGRGMTESKAYALKKGDELDRKFGYYLLPELLEQEEETRKEQTTTAMDTEDRAKLGWLLNLRACMLPDPIEGKNEVSGLELYFLEQDGKAFKTRVIYQPYFYIRPEANR-SQEVIAYCLRRFEGLVTKTERLFREDLDFADHLNGKKALYVKLSFATVTDLMMVKRELAPLIAKNQERLQVRQAYEIQGQSREKVTENDLSFGADGDTINGAMTDESETSLLELREYDVSYPMRVAIDLDIRVGAWYLVDYNPTAPYSPSDVKRQKDMVDKAEPVVCAFDIECTKAPLKFPDAQVDQIYMISYMIDKQGYLIVNRDFVSEDIQDFEYTPKVAYPGPFIVFNEENEEKLLKRFFEHVIEEQPHIFVTYNGDFFDWPFIETRAQVYGINMAREIGVSLDERTGEYRGRCSVHMDAFCWVKRDSYLPQGSQGLKAVTKYKLGYDPVEVDPEDMVALARDEPLKMASYSVSDAVATFYLYDKYVHLFVFSLCTIIPLGSEDVLRKGSGTLCEALLMVEAFKGNIICPNKQGSSREEKFYKGHLVENETYVGGHVECLESGVFRADFSYDFQLVPAAFQQLIDNIDRDLRFSLEVEMNVPLQLVTNYEEVRKDIVDRLVALRERPSRKEQPLIYHLDVAAMYPNIILTNRLQPCAIVNEADCAACTFNTECGRAAIEEKDASCQREMDWVWRGDFYPATRPEYYSIKTQIEYENFSLENNGGSTNSDPAKRINGTGSTMPFAQLPIEKQEQLLKQRFKSYCSSVYKKTKVTEEQQRSATVCMRENPFYVNTVRAFRDRRYDYKGLTKVWKKKVSEAADAKDLLAKIDAQNKCLVYDSLQLAHKCILNSFYGYVMRKGARWHSMEMAGIVTHTGSNIITGARELVEQIGRPLELDTDGIWAILPKSFPETFSFKLKDGGSRSISYPCVMLNADVNAKFTNPQYHEL-DPVTGKYSSHKECSIMFEVDGPYKAMVLPASTEEGKLLKKRYAVFNFDHSLAELKGFELKRRGELQLIKAFQSQVFECFLDGDSLEECYASVAKCANRWLDVIDTRGKSMNDEELMALITENKSMSGKLDEYEGQKSTSITTARRLGEFLGEEMIKDKGLTCKMIIAARPYGEKVTERAIPTAIFATEDAVKRHFLKKWMKDPHLQDFDIRSLLDWDYYRTRFASTVQKIISLPAAFQNVRNPVPRIELPDWMRKQVREKNATHQQGNMKQFFKTT---TKRTDNEDYVAQL--------IDMEDLMKGNQSLRPGKPIT--RKRPKK------------ASDGXXXXXXXXXXXDAENVPAVTVKLGEV----PFTNWLKQRKKKWK-----TMLGKRRSERDDRLRSFSGAD---------GFGMANSAHGSTKRFQSGHTSALEAKGNGVGMENFVFDAGSALQREYWQIVEIQ-QEQGGRFTCWILTAHSQMLQRITLKLPRVFYITCEEADHENLLSLLPGSRRVSRIVPHYSSKPQSVLEVMLSERQYQEYQKEISQILGDPCVRGVYELQVSALTRAVCAFGCVAKVVNPHISVTN---------QEYELSDLQFQSTAQAPYLLDHRRSIAANGVASPXXXXXXXXXXXXXXXXXXXXVLGLVVLKEGDDDITA--TTLGNAVSEG--------------EVNVWYVDPISNVKSRGSDLKGFFEQLLQESQEEGGIAPIQSCSFKTHVVKTAQEAFSGVNATLARLASNKQLTPKPTIVVAQTCIPSSKRLREKIQGLYSFPVAMLPWNEEDTLFPALTWRKLMGEKMLTRCFEMIQYYSDVLDCARYAHLPVGNFTGDHSLAILDTLYSRILTKHKHLWWHSPTSLPDLGGKEQEQQFQQLFSQSLLGSAQQSSLKKSDTALQHRIDTSVVVCKKGSFVDVCVELELDGLAINAILVASQIHSIEGLGVAHQMEIDFDGNGERESSKGVGPTSGVSEGDAE---------------ESCHEAFRLLRIMVTTLFKDFLTSRNKFADHMLQQFYRWLCSPNSLSFDPALQAMVKRLMEKLFLQLLAELRRLGSQIVYADFSKIIVCTNKKSVRDAQTYLNFILQTVLSNELFQVLNFTPKKYFSNLLFLDAENYGCILLKN-------------------------------------------------------------------------------------------------------LVPAGGQEEDQEGENDHNVDGSD-----EKPLEIVSHWNIANYLPRGVDEYFLILVGQFIRRRAEFQHRERRRHER---------------GGDLKPDAAEDAGEQPLD--------------------------EDEKQMSPLAKYSQRLVSSYFSEKLLRLVPEILTYNMDR-DSFPVFAGSHLVMESPALELVKFVTSVFLLEPSVETQVGKMRRTLLKLLRMSEFVDETQFRNPSLSFTVQDVICLSCNLCRSIDLCRDPQLFKGSASRSDKDVEGEEHGEDVEASSAWHCPRCFALYDMTAFEMRLVEMIQAQSVAYQLQDLYCRKCHLPAEHKMREYCPCSGTYALMPGVRESLMETLRLLMRIAQQHNFAWLLETVQRL 2403          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16166.1 vs. uniprot
Match: K3WQI5_GLOUD (DNA polymerase epsilon catalytic subunit n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WQI5_GLOUD)

HSP 1 Score: 1745 bits (4520), Expect = 0.000e+0
Identity = 1064/2620 (40.61%), Postives = 1464/2620 (55.88%), Query Frame = 0

Query:   53 ADSSQAARLS-----GGGRKESAVKAALREQGDELDKRFGYD---------------------------RYSEGPPRLGWIFNMLPTSIPDASGN--EKSGLDMYFLEQDGGTFKATVFYKPYFYVLVKEERYIQDCIQCIRRRFEDSAVEVTVVDKEDLDMPNHLSGKLRRFLKLSFHSVADLMEVKRALMPTIQANKTRAEAQDAYMHAEAREQ-QPSDFL-----------------SVLVDAREYDVPYYVRCSIDLDIRVGAWYMVTPQEEGV----EVAWQKEMVDKAEPKVLAYDIECTKAPLKFPNVEIDQVFMISYMVDGQGYLIISREVVSEDVENFEYTPKPSFPGPFHIFNEPNEEAVIRRFFNHVAELRPQIIVTYNGDFFDWPFVDERAKGYGLDMEKEIGVGLQA-NGEYRGRCVAHMDAIYWVKRDSYLPQGSHGLKAVTKYKLGYDPVEVDPEDMVRYASERPTEMAAYSVSDAVATYYLYQTYVHMFVFSLCTIIPMGPDDVLRKGSGTLCEALLMVEAYRGNIVCPNKHSEP-LTKFHDGHLVESETYIGGHVECLETGVFRSDLPEKFRLVPSALQALIDNVDRDLTFAIEVESGVQRDTVSNYDEVRSEIIEQLEMLRDSPLREEEPFIYHLDVAAMYPNIILSNRLQPGAVVNKAMCASCDFNQESNN---------CKRSLTWKWRGDMTPASRTEYQSVKMQLTYENV--------------------DGK------PFVELDEADQATNVRARLKNYAHKVYKKTKITKEEERVDTVCMRENPFYVNTVRAFRDRRYDYKILTKKAKKAKSKAAEEGDAVGAKEAGDRAQVFDSLQLAHKCILNSFYGYVMRKGARWRSMEMAGIVTYTGAMLIKQARELVEQIGRPLELDTDGIWCILPSSFPENFTFTTKSGGKIRISYPCVMLNADVHENYTNHQYQDLQDPATRKYNTKSECSIFFEVDGPYRCMVLPSSTEEGKLLKKRYAVFNHDGSLAELKGFELKRRGELEVIKVFQSQVFEKFLRGDTLAQCYDAVGEVANQWLDVLYSKGGDVDDDELMELISQNKTMSQTLEDYGTAKGTSQTTARRLADFLGAEMVKDKGLNCRMILSNRPHGAPVTDRAVPTAIFSAEPAVKKHYLRKWLKDPAMEDFDIRSILDWDYYLDRLGNCIRKIITIPAAMQKVENPCPRVKHPDWLRKIVREINDGYRQMKIDQLFKAAPPGTPQLTNAGASTKAKPTPAPAATDIEDMAGXXXXXAAGRSILGARARVTKRIGNGAAAAGALAPDGSPGKENADVGXXXXXXXXXXXXXXPIRR-EDDFQGWLAQQKAGWK----KRREQNRLKRRTEARSQAMASFRGSGSAGARGGTAGLGAAGAAEKRRRTAAMGKS-----GLQGYLTSAQDALLRGFWQVVEIRPSDNPGSFTVFAMTGPGE-LQVIPITIPRVFYVNCLEDKGPAGSST--MGRRVARTLPN--GRGSPFLYEVAMDEAKFQRNEKQVEDFLHHAGIEGVYELGTPLVYRAMLHLGSVARVVQGRAKALKGVKSGSGQAPGFELEDLELVTAAAHPYL---------HPSVATFKHIYLYHSSSGKRGMVGLFYVQGTNEDIEPEPTAEGGAEDVGGEGGLGPGERPVIAKAVVWLVNPFSR---RDGEQRPPFARIFEKFTNN-----PDSSCTFKTSSVSSADEAWAAAD---ASLASYSRQRNGPTLVLSQGLVQG-QELRRRLPALSELPVTHIPGNAEDERYPALGWQAFAAQRMVQRFFIAPRWLADRLLCARYAHLPLANIGADAPCAMADAFFGRLLRHNRHVLWASETSRPDLGGTEGDD------NDIWAEDL-----------ASPTILQPGAYRNICIELEIHGLAVGSVIASSQLEALEGSQGVTALTVNNDNEGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQAVASAFRILKVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNATLHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQLEPKMFWEQLLFLDDQDYGGIQVQGGFKEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKRDNDLKPXXXXXXEDAQVSGRGVSGADGTVAEQKMPSIVSHWNLAEYLPASVRQVFLILVSTFLLKPHEKARQLRLDHERMVASLSNQLTQSSQPNGPASPGTDRGESXXXXXXXXXVPASQGGMDAALAVQAALRKEEANESQS-MADFLKHLVTRTLTDKVLETVPAIKKYSGSGPDAFPNKPGAARELTNPALEFIKSLCHVLSLDPTVTDEVETLRKQALAEVGVRQFSAEGQYSDPCVSFVLPDVICSFCNLCRDLDLCRDSRLM------------------DEDEDER---WQCIQCNHPYERSSIELSLVEAVQRRSVRYQLQDMKCAKCGAVATRSMSLTCPCSGPLV-AEESQQEFHKTMKLLRQLAIFHNFGWLLETVENI 2503
            A +SQ AR +     GG R  +  K  + ++GD LDK+FG+                              +E  P+LGW+ N+   SI D +    E S +++YFLEQDG  FK  V Y+PY +V  +E R  Q+ I    R+FE    +   V KEDLDMPNHLSGK   ++KLSF++V+DL+ VK+AL P ++ N+ + +A+ AY     RE+    D L                 S L++ REYDVPY +R +IDL+IRVGAWY V           ++    +MVDKAEP V A+DIE TK PLKFP+ + DQ++MISYM+D QGYLI++RE VSED+++FEYTPK  +PGPF +FNE NEE +++RFF HV + +P I VTYNGDFFDWPF+D R+K +G++M  EIG+ L   +GEYRGR   HMDA  WVKRDSYLPQGS GLKAVTKYKLGYDPVEVD EDM+  A + P +MA+YSVSDAVAT+YLY+ YVH+FVFSLCTIIP+G +DVLRKGSGTLCEALLMVEA+ G I+CPNKHS     KF+  HLVESETY+GGHVECLE+GVFR+D    F LVPSA Q LIDN+DRDL F++ VE  V  D V+NYDEV+++I+ QLE LRD P R+E+P IYHLDVAAMYPNIIL+NRLQP A+VN+A CA+C +N E  N         C+R + W WRGD  PA+R EY S+K QL YEN                     +G       PF +L+E  Q   ++ R K+Y   VYKKTKIT++E+R  TVCMRENPFYVNTVRAFRDRRYDYK LTK  KK +++A  + D +   EA ++  V+DSLQLAHKCILNSFYGYVMRKGARW SMEMAGIVT+TG+ +I  AR+LVEQIGRPLELDTDGIW ILP+SFPE F+F    G    ISYPCVMLNADV+  +TN QY +L DP T KY T  ECSI FEVDGPY+ MVLP+STEEG+LLKKRYAVFN D SLAELKGFELKRRGEL++IK FQ QVFE FL G+ L +CY +V + AN+WLDV+ +KG  +D++ELMELI++NK+MS  LE+Y   K TS TTA+RL++FLG  MVKD+GL C+MI++ +P+G  VT+RA+PTAIFSAE AVK+H+L+KWLKDP ++DFDIR+ILDW+YY  R  + I+KII++PAA Q V+NP PR++ PDW+RK VRE N  ++Q  + +   +        TNA     A+        D+ED+A           +L  + +  KR      ++     + +    N                  P++  E  F  WL  +K  WK    KR+ Q   + R  A   +M                G G+ G + KR +T A  ++     G++ ++ +   AL R FWQ+VE++  +  G FT + +T   + L  I + +PRVFY+ C E      +      RRV R +P+   +  P + E+ + E +FQ+N K++   L+   I+GVYEL    + RA+  LG V +VV     A             +EL DL+  + A  PYL         +      + + LY +    R ++GL  ++   ED   + + EG                     A+VW V+P S    R  E    F ++ +             SC+FKT  V S  +A++  +   A LAS  +    PT+V++Q  +   + LR ++  L   P+  +P N ED  +PAL W+    ++M+ R F   ++  + L CARYAHLP+ N   D   A+ D  + R+L  N+H+ W S +S PD GG E +       N + A+              S  +   G+Y +IC+ELE+ GLAV +++ ++Q++++EG  G   + ++ DN+                                    +    AFR+L++LVTT   +     N  AD LL  FYRW+C+  SL ++  L  +V       F +L+AE RRLG+ +V+A F K+II T K D+K A+ Y +F++ TV S +LF  L   PK ++  L FLD ++YGGI ++   ++                                                                                                D D +       E  Q SG      D  +       I+SHWN+A YLP  + + FLILV  F+ +  E   QLR +  ++++  S+Q+ ++           D  E+                             +EA +  S +A + + LV    ++K+L  VP I+ Y+    D+FP   G+   + +PALE +K +  V  L+P+V  ++  +R+  L  + V +FS + Q+ +P +SF +PDVIC  CNLCR +DLCRD +L                   D+D +     W C +C   Y++ + E  LVE V+ +SV+YQLQD+ C KC   A   M   C CSG       +++E  +T+++LR++A  H F WLLETV+ +
Sbjct:   26 ARNSQGARANAQSNRGGNRSMTDAKQFMLKKGDALDKKFGFHLLPELIEQEQQDASSGEMTTSASSSSANANERDPKLGWLLNLRACSILDPTEGKFEVSAVELYFLEQDGKAFKTRVIYQPYLFVRPEENR-SQEVIAYCLRKFEGLIAKAERVFKEDLDMPNHLSGKKAVYVKLSFYTVSDLVTVKQALAPIVEKNQAQQKARQAYELQGTREKVNEEDQLFGGDSLTLSRGGMDESESALLELREYDVPYSMRVAIDLNIRVGAWYHVNYDPAAPYAPSDIKRLPDMVDKAEPVVCAFDIETTKLPLKFPDAQFDQIYMISYMIDRQGYLIVNREFVSEDIQDFEYTPKKDYPGPFIVFNEENEEQLLKRFFEHVVDAQPHIFVTYNGDFFDWPFIDARSKFHGINMADEIGISLDIRSGEYRGRSSVHMDAFCWVKRDSYLPQGSQGLKAVTKYKLGYDPVEVDAEDMLPLARDDPLKMASYSVSDAVATFYLYEKYVHLFVFSLCTIIPLGSEDVLRKGSGTLCEALLMVEAFEGGIICPNKHSSAHQEKFYKSHLVESETYVGGHVECLESGVFRADFLYDFNLVPSAFQHLIDNIDRDLRFSLVVEMDVALDRVTNYDEVKAKIVGQLEALRDRPTRKEKPLIYHLDVAAMYPNIILTNRLQPCAIVNEADCAACVYNTECGNSAVKDQDESCQRDMDWIWRGDFYPATRPEYFSIKTQLEYENFPVGPQGGTVAAGGPSKFGGDNGSNTTRMVPFGQLNEDRQEQLIKQRFKSYCSSVYKKTKITEQEKRAATVCMRENPFYVNTVRAFRDRRYDYKNLTKTWKKKQAQAEAQNDLLAKIEAQNKCLVYDSLQLAHKCILNSFYGYVMRKGARWHSMEMAGIVTHTGSNIITGARQLVEQIGRPLELDTDGIWAILPASFPETFSFKLNDGSSRSISYPCVMLNADVNAKFTNPQYHEL-DPETGKYKTHQECSILFEVDGPYKAMVLPASTEEGRLLKKRYAVFNFDHSLAELKGFELKRRGELQLIKAFQGQVFECFLDGENLEECYASVAKCANRWLDVIDTKGKSMDNEELMELITENKSMSGKLEEYEGQKSTSITTAKRLSEFLGESMVKDRGLTCKMIIAAKPYGEKVTERAIPTAIFSAEEAVKRHFLKKWLKDPNLQDFDIRTILDWEYYRGRFASTIQKIISLPAAFQNVKNPVPRIELPDWMRKQVREKNAKHQQGSLAKFVTST---KKDKTNANEDYIAQ------LIDLEDLALGKKPSDRPNKLLSLKQK-RKRAAESGNSSAESTEELNEASSNTSAAEQDPIEDVDMVPAAPVKLGEVAFSEWLKNRKTKWKTLLVKRKRQREERARNIAGGNSMIEL-------------GSGSIGPSAKRHQTMAASRTTGGGIGMENFVLNTASALHREFWQIVEVQ-QEQDGHFTCWVITTQSQMLHRITLKMPRVFYLACDEADSHIFTDLPLFTRRVLRIIPHLSSKLQPVV-EIMVSEQQFQQNHKELNQILNDPVIKGVYELQVSALTRAVCSLGCVVKVVNPHHSAAN---------QEYELSDLQFQSTAQVPYLVDLYQGTSGNNPAPLMRRVLLYFAQQKNRAILGLVVLKDAGEDSSSD-SCEG--------------------DAIVWYVDPVSNMKVRGSELLQYFGQLLQSSVEEGVESVTMQSCSFKTHVVKSVQDAFSGVNSALARLASNKQMTAKPTIVIAQTCIPSTKRLREKIQGLHSFPMAMLPWNEEDTLFPALTWRKLMGEKMIARCFEMAQYFQEVLDCARYAHLPVGNFTGDHSIAILDTLYSRILTKNKHLWWHSPSSVPDFGGKEQEQYQHLFSNSLLAKAQHGKANATHRTDTSAVVCDKGSYPDICVELELDGLAVNAILVAAQIQSIEGIGGAHQMEIDFDND----------------DASGANAAGAKSSVAEGEAEEPCQEAFRLLRLLVTTLFKDFLTSRNKFADHLLQQFYRWVCSSNSLCYDPGLQAMVKRLMEKLFLQLLAEFRRLGSQIVYADFSKIIICTKKQDIKNAQTYLQFILQTVLSNELFQVLSFTPKKYFSNLFFLDSENYGGILLKNVSEQDA----------------------------------------------------------------------------------------------DEDEE-------ESDQASG------DAPL------DIISHWNIANYLPKGIDEYFLILVGQFIRRRAEF--QLR-ERAKLLSGASSQMNKA-----------DEQENEEVL-------------------------DEAEKKLSPLAKYSQWLVASYFSEKLLRIVPEIQSYNMDR-DSFPVFAGSHLLMEHPALELVKFVSAVFQLEPSVEAQLGKMRRTLLKLLHVSEFSDDAQFVNPSLSFAVPDVICLCCNLCRSVDLCRDPQLFKGSSSRSNPDASDGDDAGDQDPNSAVSSWHCPRCFALYDKHAFEQRLVEMVEAQSVQYQLQDLYCQKCYLPAEHKMREYCTCSGTYGHLPGAKEELQETLRILRRIAQQHEFEWLLETVQRL 2419          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16166.1 vs. uniprot
Match: A0A2R6XQA7_MARPO (DNA polymerase epsilon catalytic subunit n=1 Tax=Marchantia polymorpha TaxID=3197 RepID=A0A2R6XQA7_MARPO)

HSP 1 Score: 1732 bits (4485), Expect = 0.000e+0
Identity = 1025/2487 (41.21%), Postives = 1396/2487 (56.13%), Query Frame = 0

Query:   80 DELDKRFGYDRYSEGPPRLGWIFNMLPTSIPDASGNEK--SGLDMYFLEQDGGTFKATVFYKPYFYVLVKEERYIQDCIQCIRRRFEDSAVEVTVVDKEDLDMPNHLSGKLRRFLKLSFHSVADLMEVKRALMPTIQANKTRAEAQDAY------MHAEARE-QQPSDFLSVLVDAREYDVPYYVRCSIDLDIRVGAWYMVTPQEEGVEVAWQKEMVDKAEPKVLAYDIECTKAPLKFPNVEIDQVFMISYMVDGQGYLIISREVVSEDVENFEYTPKPSFPGPFHIFNEPNEEAVIRRFFNHVAELRPQIIVTYNGDFFDWPFVDERAKGYGLDMEKEIGVGLQA-NGEYRGRCVAHMDAIYWVKRDSYLPQGSHGLKAVTKYKLGYDPVEVDPEDMVRYASERPTEMAAYSVSDAVATYYLYQTYVHMFVFSLCTIIPMGPDDVLRKGSGTLCEALLMVEAYRGNIVCPNKHSEPLTKFHDGHLVESETYIGGHVECLETGVFRSDLPEKFRLVPSALQALIDNVDRDLTFAIEVESGVQRDTVSNYDEVRSEIIEQLEMLRDSPLREEEPFIYHLDVAAMYPNIILSNRLQPGAVVNKAMCASCDFNQESNNCKRSLTWKWRGDMTPASRTEYQSVKMQL-------TYENVDGKPFVELDEADQATNVRARLKNYAHKVYKKTKITKEEERVDT--VCMRENPFYVNTVRAFRDRRYDYKILTKKAKKAKSKAAEEGDAVGAKEAGDRAQVFDSLQLAHKCILNSFYGYVMRKGARWRSMEMAGIVTYTGAMLIKQARELVEQIGRPLELDTDGIWCILPSSFPENFTFTTKSGGK-IRISYPCVMLNADVHENYTNHQYQDLQDPATRKYNTKSECSIFFEVDGPYRCMVLPSSTEEGKLLKKRYAVFNHDGSLAELKGFELKRRGELEVIKVFQSQVFEKFLRGDTLAQCYDAVGEVANQWLDVLYSKGGDVDDDELMELISQNKTMSQTLEDYGTAKGTSQTTARRLADFLGAEMVKDKGLNCRMILSNRPHGAPVTDRAVPTAIFSAEPAVKKHYLRKWLKDPAMEDFDIRSILDWDYYLDRLGNCIRKIITIPAAMQKVENPCPRVKHPDWLRKIVREINDGYRQMKIDQLFKAAPPGTPQLTNAGASTKAKPTPAPAATDIEDMAGXXXXXAAGRSILGARARVTKRIGNGAAAAGALAPDGSPGKENADVGXXXXXXXXXXXXXXPIR--------REDDFQGWLAQQKAGWKKRREQNRLKRRT--EARSQAMASFRGSGSAGARGGTAGLGAAGAAEKRRRTAAMGK--SGLQGYLTSAQDALLRGFWQVVEIRPSDNPGSFTVFAMTGPGELQVIPITIPRVFYVNCLEDKGPAGSSTMGRRVARTLPNGRGSPFLYEVAMDEAKFQRNEKQVEDFLHHAGIEGVYELGTPLVYRAMLHLGSVARVVQGRAKALKGVKSGSGQAPGFELEDLELVTAAAHPYLHPSVATFKHIYLYHSSSGKRGMVGLFYVQGTNEDIEPEPTAEGGAEDVGGEGGLGPGERPVIAKAVVWLVNPFSRRDG-----EQRPPFARIFEKFTNNPDSSCTFKTSSVSSADEAWAAADASLASYSRQRNGPTLVLSQGLVQGQELRRRLPALSELPVTHIPGNAEDERYPALGWQAFAAQRMVQRFFIAPRWLADRLLCARYAHLPLANIGADAPCAMADAFFGRLLRHNRHVLWASETSRPDLGGTEGDDNDIWAEDLASPTILQPGAYRNICIELEIHGLAVGSVIASSQLEALEGSQGVTALTVNND-----NEGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQAVASAFRILKVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNATLHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQLEPKMFWEQLLFLDDQDYGGIQVQGGFKEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKRDNDLKPXXXXXXEDAQVSGRGVSGADGTVAEQKMPSIVSHWNLAEYLPASVRQVFLILVSTFLLKPHEKARQLRLDHERMVASLSNQLTQSSQPNGPASPGTDRGESXXXXXXXXXVPASQGGMDAALAVQAALRKEEANESQSMADFLKHLVTRTLTDKVLETVPAI------KKYSGS----GPDAFPNKPGAARELTNPALEFIKSLCHVLSLDPTVTDEVETLRKQALAEVGVRQFSAEGQYSDPCVSFVLPDVICSFCNLCRDLDLCRDSRLMDEDEDERWQCIQ--CNHPYERSSIELSLVEAVQRRSVRYQLQDMKCAKCGAVATRSMSLTCPCSGPLVAEESQQEFHKTMKLLRQLAIFHNFGWLLETVENILVADGLVEE 2512
            D L+ +FG+D ++EG PRLGW+ NM  +S+ D+  + K  S +++YF+ QDG TFK+ V ++PYFY+  KE   ++     +RRR+E     + +V+KEDLDM NHLSG    FLK+SF++V +LM VK  L+P ++ N+ + EA  AY      M    +E     DF+  +++ RE+DVPY+VR +ID D+R G WY VT +   V++  + +++ +AE ++ A+DIE TK PLKFP+   D V MISYM+DGQGYLI++RE V ED+++ EYTPKP + G F + N PNE+AV+  +FNH+ +++P I VTYNGDFFDWPF+++R+K +G+ M +EIG    A  GE   +   H+D   WVKRDSYLPQGS GLKAVTK KLGYDP+EV+PEDMVR+A E+P  M++YSVSDAV+TYYLY TYVH F+FSL TI+PM PD+VLRKGSGTLCE LLMVEA++GNI+CPNKH     K ++G +++SETYIGGHVECLE+GVFRSDLP KFRLVP A Q LID +DRDL +AIEVE  +  D+V+NYDEV+  I  QLE LRD+P REE P IYHLDVAAMYPNIIL+NRLQP ++V   +CA+CDFN+    C R L W WRG+   ASR+EY  +K Q+       T E  D + F +L + +Q + ++ RLK Y  KVYK+  + K    V T  +CMRENPFYV+TVR+FRDRRY+YK L K  K    +A   G+ +  +EA D   V+DSLQLAHKCILNSFYGYVMRKGARW SMEMAG+VTYTGA +I+ A +LVEQIG+PLELDTDGIWC LP SFPENF F T    K + ISYPCVMLN DV  N TN QYQ + DP +R Y T SECSI FEVDGPY+ M+LP+S EEG L+KKRYAVFN DG+LAELKGFE+KRRGEL++IKVFQ++VFE FL+GDTL +CY AV E+AN+WLD+L ++G D+ D EL++ IS++ TMS++LE+YG  K  + TTA+RLADFLG  MVKDKGL+CR I++  P GAPV++RA+P AIF  EPA+ K YL+KW K  +  D   RSI+DW YY  RL + I+KIITIPAAMQKV NP PRV HP+WL K VRE +D YRQ  +  +F+  P   P                    D+EDM          R  L A +     +            D  P +E   +G              P          +E D++ W+  +K   K+ REQ + +R         A+ +                          R    GK  +G+     S Q AL R  WQ++++ P+ +PG F  + +   GE+  + I +PRVFY+N    + P  + + G+RV R LP+ +    L EV  DEA+F+   K +   L    +EGVYE     + +A+L LG V  V    AKA K   + +     + +++L + T A   YL  ++ +F   ++Y S    RG+  L+                                 P   K +V +VNP + ++      E++   A      +N   S+ T K   +SS  +A    + SL  +  Q  GPT+ L+Q     ++L   +P L+E P   IP N  D +Y ALGWQ  A +  + R   +  WL +R+  ARYAH+PL N+ +D     AD F+ R LR N+ VLW+S+   PDLGG   DD+    +++  P +  PGAYRN+ IE +I+ LAV +++ SSQ+   EG       TV+ D     N+G+                                     + AFR+LK LV  WL +    GN ++D+LL H YRWIC+ QS LH+  L R++       F+ L+ ELR+LGA +VFA F K+IIATNKS++  A+ Y ++++ TV +R+LF  + L PK +W  LLF+D  +YGGI+                                                                                                                     A  S       +GT+ E+    IVS+WN+AEYLP   +  F+++VS ++  P + A            S +  +T                                          AA    EA E      FLK  V    T+K+L+TV  +      KKY           FP   G+  E+T+ ALEFIK +C V  LD  V  EV  LRK  L    VR+FS E  + DPC+SF+LP+VICS+CN CRD+DLCRD+ L++ D    W+C+   C  PY R  +E +L++ V++R   Y LQD+ C KC  V    ++  C C+G     ES ++F + M++   +A++H F  L E V  IL    + E+
Sbjct:   60 DALEAKFGFDVFAEGEPRLGWLLNMAASSMQDSEDDSKTFSCVNLYFMCQDGSTFKSQVKFQPYFYLGTKENTELE-VDAYLRRRYEGQIASIQIVEKEDLDMKNHLSGTRHTFLKVSFNNVQELMSVKSELLPIVERNQAKVEAAQAYNTLFQGMRGGLQETSNVQDFVDYMLEVREFDVPYHVRFAIDKDVRCGLWYTVTVESNKVKLEKRADLLQRAEARICAFDIETTKLPLKFPDASYDSVMMISYMIDGQGYLIVNRECVGEDIQDLEYTPKPEYEGFFQVTNAPNEKAVLETWFNHMRDVKPGIYVTYNGDFFDWPFLEQRSKHHGMSMYEEIGFRCDAVQGECLSKFACHLDCFAWVKRDSYLPQGSQGLKAVTKAKLGYDPLEVNPEDMVRFAMEKPQVMSSYSVSDAVSTYYLYMTYVHPFIFSLATILPMPPDEVLRKGSGTLCEMLLMVEAFKGNIICPNKHKSEEEKVYNGRILDSETYIGGHVECLESGVFRSDLPTKFRLVPGAYQRLIDKLDRDLKYAIEVEGKMDLDSVTNYDEVKHNISTQLEALRDAPNREETPLIYHLDVAAMYPNIILTNRLQPPSIVTDDVCAACDFNRPDKTCLRQLEWVWRGETFSASRSEYNHLKNQIESEQFPPTSEGGDMRYFRDLPKDEQQSKLKDRLKKYCQKVYKRV-LDKPISDVRTAGICMRENPFYVDTVRSFRDRRYEYKGLNKVWKGKLGEAKASGNPIKIQEAQDMVVVYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNAHKLVEQIGKPLELDTDGIWCCLPGSFPENFVFKTSDPKKKLPISYPCVMLNVDVAVNNTNDQYQTMVDPVSRTYATSSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAEVFENFLQGDTLEECYAAVAEIANRWLDMLENQGLDLADSELLDYISESSTMSKSLEEYGDRKSCAMTTAKRLADFLGDAMVKDKGLSCRYIVAKDPPGAPVSERAIPVAIFETEPAIMKAYLKKWCKSSS--DVVFRSIVDWAYYRQRLSSAIQKIITIPAAMQKVSNPVPRVTHPEWLWKKVREKDDKYRQKSLTDMFQKKPAALPDGIE----------------DLEDMCKTSEKH---RPTLSAPSHKKGDL-----------IDEIPLQEGECIGQEDIPPIEKSLNEDPENEARSKTPDKEVDYEAWVEYKKLKRKETREQRKRQREVIDPTGDDAVVAL--------------------PRNVSRKDGKGKRQAGVGAMFRSQQAALTRSHWQLLQLVPTQSPGFFRAWILVA-GEMYKLQIQVPRVFYINT---RAPPDTES-GKRVNRVLPHMKPCFNLLEVVCDEAQFKDARKFLSAHLADPDVEGVYETKISPLQQAILRLGCVCTV---DAKASKRNANAT-----WSVDELHMKTTAESAYLKVALNSF---FIYQSCMNTRGLYTLY--------------------------------TPAAEKILVVVVNPRNNKEVSSVQLEKQFRDAASNSGLSNLEVSTWTVKVEYLSSHQDAGRLLNRSLVEFRDQHPGPTVALTQ-CPNTEDLMAIIPLLNEFPCVQIPCNVRDSQYQALGWQPIAGRVAMHRCVASSVWLQERISLARYAHVPLGNVDSDWLLFTADTFYSRALRENQQVLWSSDDGVPDLGGAITDDSTSLEDEVQQPAVSFPGAYRNVTIEFKIYHLAVNALLKSSQISEFEGG------TVDGDGSAAFNDGSNTWNEEIFC----------------------------SPAFRVLKQLVGGWLADAISTGNNYSDALLQHVYRWICSPQSKLHDPALQRVLHKVMQKVFALLLMELRKLGATIVFADFNKIIIATNKSNITMAQGYCEYLLKTVKARELFEWIDLRPKQYWHSLLFMDQHNYGGIKANT------------------------------------------------------------------------------------------------------------------ATQSFEHQEDQNGTLPEE----IVSYWNIAEYLPKVTQDYFVVIVSEYIYIPWKHAMDEAARRRAGPVSSTPSIT-----------------------------------------VAAAADMEAGEIL----FLKEKVQSHFTEKLLKTVRDVQRHMNTKKYEDDEELQSAREFPKLAGSHLEMTDAALEFIKHVCAVFMLDQRVQHEVLVLRKNLLKLARVREFSQEATFRDPCLSFILPNVICSYCNDCRDMDLCRDAALIERD----WRCVMPLCGQPYNREWVENALLQIVRKRERLYHLQDLLCVKCRQVKASHLAEQCTCAGNFRCRESPKDFLQRMRVFHNIAVYHGFDLLQECVSWILQTAAIPED 2242          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16166.1 vs. uniprot
Match: B9SSU6_RICCO (DNA polymerase epsilon catalytic subunit n=3 Tax=Euphorbiaceae TaxID=3977 RepID=B9SSU6_RICCO)

HSP 1 Score: 1731 bits (4482), Expect = 0.000e+0
Identity = 1026/2471 (41.52%), Postives = 1384/2471 (56.01%), Query Frame = 0

Query:   67 KESAVKAALREQGDELDKRFGYDRYSEGPPRLGWIFNMLPTSIPDASGNE-KSGLDMYFLEQDGGTFKATVFYKPYFYVLVKEERYIQDCIQCIRRRFEDSAVEVTVVDKEDLDMPNHLSGKLRRFLKLSFHSVADLMEVKRALMPTIQANKTRAEAQDAY--MHAEAREQQPSDFLSVLVDAREYDVPYYVRCSIDLDIRVGAWYMVTPQEEGVEVAWQKEMVDKAEPKVLAYDIECTKAPLKFPNVEIDQVFMISYMVDGQGYLIISREVVSEDVENFEYTPKPSFPGPFHIFNEPNEEAVIRRFFNHVAELRPQIIVTYNGDFFDWPFVDERAKGYGLDMEKEIGVGLQAN-GEYRGRCVAHMDAIYWVKRDSYLPQGSHGLKAVTKYKLGYDPVEVDPEDMVRYASERPTEMAAYSVSDAVATYYLYQTYVHMFVFSLCTIIPMGPDDVLRKGSGTLCEALLMVEAYRGNIVCPNKHSEPLTKFHDGHLVESETYIGGHVECLETGVFRSDLPEKFRLVPSALQALIDNVDRDLTFAIEVESGVQRDTVSNYDEVRSEIIEQLEMLRDSPLREEEPFIYHLDVAAMYPNIILSNRLQPGAVVNKAMCASCDFNQESNNCKRSLTWKWRGDMTPASRTEYQSVKMQLTYENVDG------KPFVELDEADQATNVRARLKNYAHKVYKKTKITKE--EERVDTVCMRENPFYVNTVRAFRDRRYDYKILTKKAKKAKSKAAEEGDAVGAKEAGDRAQVFDSLQLAHKCILNSFYGYVMRKGARWRSMEMAGIVTYTGAMLIKQARELVEQIGRPLELDTDGIWCILPSSFPENFTFTTKS-GGKIRISYPCVMLNADVHENYTNHQYQDLQDPATRKYNTKSECSIFFEVDGPYRCMVLPSSTEEGKLLKKRYAVFNHDGSLAELKGFELKRRGELEVIKVFQSQVFEKFLRGDTLAQCYDAVGEVANQWLDVLYSKGGDVDDDELMELISQNKTMSQTLEDYGTAKGTSQTTARRLADFLGAEMVKDKGLNCRMILSNRPHGAPVTDRAVPTAIFSAEPAVKKHYLRKWLKDPAMEDFDIRSILDWDYYLDRLGNCIRKIITIPAAMQKVENPCPRVKHPDWLRKIVREINDGYRQMKIDQLFKAAPPGTPQLTNAGASTKAKPTPAPAATDIEDMAGXXXXXAAGRSILGARARVTKRIGNGAAAAGALAPDGSPGKENADVGXXXXXXXXXXXXXXPIRREDDFQGWLAQQKAGWKKRREQNRLKRRTEAR--SQAMASFRGSGSAGARGGTAGLGAAGAAEKRRRTAAMGKSGLQGYLTSAQDALLRGFWQVVEIRPSDNPGSFTVFAMTGPGELQVIPITIPRVFYVNCLEDKGPAGSSTMGRRVARTLPNGRGSPFLYEVAMDEAKFQRNEKQVEDFLHHAGIEGVYELGTPLVYRAMLHLGSVARVVQGRAKALKGVKSGSGQAPGFELEDLELVTAAAHPYLHPSVATFKHIYLYHSSSGKRGMVGLFYVQGTNEDIEPEPTAEGGAEDVGGEGGLGPGERPVIAKAVVWLVNPFSRRDGEQRPPFA-RIFEKFT-------NNPDSSCTFKTSSVSSADEAWAAADASLASYSRQRNGPTLVLSQGLVQGQELRRRLPALSELPVTHIPGNAEDERYPALGWQAFAAQRMVQRFFIAPRWLADRLLCARYAHLPLANIGADAPCAMADAFFGRLLRHNRHVLWASETSRPDLGGTEGDDNDIWAEDLASPTILQPGAYRNICIELEIHGLAVGSVIASSQLEALEGSQGVTALTVNNDNEGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQAVASAFRILKVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNATLHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQLEPKMFWEQLLFLDDQDYGGIQVQGGFKEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKRDNDLKPXXXXXXEDAQVSGRGVSGADGTVAEQKMPSIVSHWNLAEYLPASVRQVFLILVSTFLLKPHEKARQLRLDHERMVASLSNQLTQSSQPNGPASPGTDRGESXXXXXXXXXVPASQGGMDAALAVQAALRKEEANESQSMADFLKHLVTRTLTDKVL----ETVPAIKKYSGSGPD----AFPNKPGAARELTNPALEFIKSLCHVLSLDPTVTDEVETLRKQALAEVGVRQFSAEGQYSDPCVSFVLPDVICSFCNLCRDLDLCRDSRLMDEDEDERWQCI--QCNHPYERSSIELSLVEAVQRRSVRYQLQDMKCAKCGAVATRSMSLTCPCSGPLVAEESQQEFHKTMKLLRQLAIFHNFGWLLETVENIL 2504
            + S  +  +R   +ELD + G+D ++EG  RLGW+     +S  D       S +D+YF+ QDG  FK+   ++PYFY   KE+  + D    +RRR+E    ++ +V+KEDLD+ NHLSG  +R+LK+SF +V  LM+VK+ L   ++ N+ + +  DAY  +    REQ+P DFL  +VD REYDVPY+VR +ID DIR G WY V+    GV +  + +++ +AE ++ A+DIE TK PLKFP+ + D + MISYM+DGQG+LII+RE V ED+E+ EYTPKP F G F + N  NE  +++++F H+ E++P I VTYNGD+FDWPF++ RA  +G+ M  E+G     N GE R R   H+D   WVKRDSYLPQGSHGLKAVTK KLGYDP+EV+PEDMVR+A E+P  MA+YSVSDAVATYYLY TYVH F+FSL TIIPM PD+VLRKGSGTLCE LLMV+AY+ N++CPNKH     KF+  HL++SETYIGGHVECLE+GVFRSDLP  F+L PSA   LI N+DRDL +AI VES +  D+VSNYDEV++ I+E+L MLRD P+REE P IYHLDVAAMYPNIIL+NRLQP ++V   +C +CDFN+    C R L W WRG++  A++++Y  +K Q+  E VDG      KPF++L + +Q + ++ RLK Y  K YK+  I K   E R   +CMRENPFYV+TVR+FRDRRY+YK L K  K   S+A   G+ +  +EA D   ++DSLQLAHKCILNSFYGYVMRKGARW SMEMAG+VTYTGA +I+ AR LVE+IG+PLELDTDGIWC+LP SFPENFTF TK    K+ ISYPCVMLN DV  N TN QYQ L DP  + Y T SECSI FEVDGPY+ M+LP+S EEG L+KKRYAVFN DG+LAELKGFE+KRRGEL++IKVFQ+++F+KFL G TL  CY AV  VAN+WLD+L ++G D+ D EL++ IS++ TMS++L DYG  K  + TTARRLADFLG  MVKDKGL C+ I++  P G PV++RAVP AIF  +  + K YLRKW K     D  IRSI+DW YY  RL + I+KIITIPAAMQKV NP PRV HPDWL K VRE  D +RQ K+  +F+ +        N    T           D+ED           R I+   A  T    +   A G +            V                I R  D+QGWL  +K  WK   E+ + +R    R  S A   F    S                    R  A  ++G+  Y T+ + AL R  WQ++++ P  + G F  + +   G +  +PIT+PRVFY+N    K P      GRRV +TLP+GR S  L EV +DE KF++  K++   L    +EG+YE   PL + A+L +G V +V        K  K  + Q   + L +L++ T     YL  S++ F   YLY S S  R +V ++                                 P +    V +VNP+  +D    P F  R F +           P ++  FK   V    +A      ++     + +GPTL + +       ++  +PAL   P   IP NA D +Y  LGWQ  AA+  +QR   + +WL +R+  +RYAH+PL N   D     AD F  R LR  + +LW S+   PDLGG   +++  +A+++  P +  PGAYR + +EL+IH LAV +++ SSQ+  +EG      L  + D                                       + A A R+LK L+   L +    G+  AD+LL H YRW+C+ QS LH+  LHR++       F+ L+AE R+LGA ++FA+F KVII T K  + AA+ Y   ++ T+ +R+LF  ++LEP  FW  LLF+D  +YGGI  +                                                                                                                     +S     GA     E ++  IVS WN+AEYLP +++  F+++VS F+  P E A++     +   ASL  Q   S  P+                                + + AA    E  ES  + ++LK  ++   TDK+L    ETV  +K  + S  D     FP   G   +   PALEFIK +C VL+LD  V  EV  +RK  L  V VR+F+ E ++ DPC SFVLP+VICS+CN CRDLDLCRD+ L+ ++    W+C   QC  PY+R  +E +L++ V++R   Y LQD+ C +C  V    ++  C C+G    +E   EF   M++   +AI   F  L E    IL
Sbjct:   16 RSSKKQKLIRNAEEELDSKLGFDLFTEGDKRLGWLLTFASSSWEDEETRRVHSCVDLYFVSQDGSAFKSKFKFRPYFYAATKEKMEM-DVEAYLRRRYESQIADIEIVEKEDLDLKNHLSGLRKRYLKISFSTVQQLMDVKKDLSHVVERNRAKFDTADAYESLLTGRREQRPQDFLDCIVDLREYDVPYHVRFAIDNDIRCGQWYDVSVSSSGVMLERRTDLLQRAEVRICAFDIETTKLPLKFPDADYDLIMMISYMIDGQGFLIINRECVGEDIEDLEYTPKPEFEGCFKVTNVKNEVELLKQWFAHMQEVKPGIYVTYNGDYFDWPFLESRAAYHGIKMSDEVGFSCDKNQGECRARSACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRFAKEKPQTMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYKANVICPNKHQSDPEKFYKNHLLDSETYIGGHVECLESGVFRSDLPTHFKLDPSAYDQLIGNLDRDLQYAIIVESKMDLDSVSNYDEVKNAIMEKLVMLRDEPVREECPLIYHLDVAAMYPNIILTNRLQPPSIVTDEICTACDFNRPDKTCLRKLEWVWRGEIFMANKSDYYHLKKQIESEFVDGIDGQLSKPFLDLPKVEQQSKLKERLKKYCQKAYKRV-IDKPVTELREAGICMRENPFYVDTVRSFRDRRYEYKGLNKLWKGKLSEAKTSGNPIKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLVEKIGKPLELDTDGIWCVLPGSFPENFTFKTKDLKKKLTISYPCVMLNVDVARNNTNEQYQTLVDPLNKTYATHSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLHGSTLEGCYSAVAAVANRWLDLLDNQGKDIADSELLDYISESSTMSKSLVDYGQQKSCAVTTARRLADFLGDTMVKDKGLRCQYIVACEPKGTPVSERAVPVAIFETDAEIMKFYLRKWCKTST--DIGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPDWLHKKVREKEDKFRQRKLVDIFRLSSKEDFVRKNNDTITDNCMMDEENVEDLEDFRNKSISTNGPRPIVHCYA--TNSEEHSVKATGRMDSSHQQTDNGESVHELSSLVQQTASSGESIDRNVDYQGWLELKKRKWKDTLERRKRQRLGNLRNSSHANGDFEPLSSV-----------------INRKKAQSRTGVGSYFTTHEIALTRCHWQIIQLFPGSHCGEFFAWVVV-EGIMLKVPITVPRVFYLN---SKAPITEKFPGRRVNKTLPHGRHSYNLVEVIIDEDKFKKESKKLAALLADPEVEGIYETKVPLEFNAILQIGCVCKVD-------KTAKKRNAQ-DRWSLNELQMKTTTECSYLEQSISFF---YLYQSISEGRAVVVVYL--------------------------------PFLRTVSVVVVNPYQNKD--LSPSFLERQFREACIALSVEPPTPRNAIVFKVDYVGHVKDAERILQGTIREVRDKHHGPTLAVIE-CPNDHLMKSGIPALDNFPCVRIPSNARDSQYQVLGWQQAAAKTAMQRSAASSQWLNERITLSRYAHVPLGNFELDWLIFTADVFISRALRDQQQLLWISDDGVPDLGGIS-EESSCFADEVHQPILTYPGAYRKVSVELKIHHLAVNALLKSSQVNEMEGG---ALLGFDQD----------------------MSSGSHICEQYGVDEATSSAPALRVLKQLIQRCLADAVTSGSVFADALLQHLYRWLCSPQSKLHDPALHRVLHKVMQKVFALLLAEFRKLGATIIFANFSKVIIDTGKFSLSAAKAYCDSLLKTLQTRELFEWIELEPLQFWHSLLFMDQYNYGGIPARAD------------------------------------------------------------------------------------------------------------------DISHSESQGAHSQRQESQV-DIVSSWNIAEYLPKTIQDHFVLIVSEFMFIPWEYAQK----QDANKASL--QDVSSCTPS--------------------------------ITISAA----ETYESH-IVEYLKGQISTYFTDKLLGIVRETVLHMKGINKSENDQPSSGFPQVAGNIHKRV-PALEFIKHVCAVLALDLNVQHEVLVMRKNLLKYVRVREFAPEAEFHDPCPSFVLPNVICSYCNDCRDLDLCRDTALLAQE----WRCAVPQCGQPYDREGMENALLQIVRQRERLYHLQDLVCIRCNQVKAAHLAEQCACAGAYRCKEDVSEFRSKMQIFLNVAISQKFQLLQECTSWIL 2224