prot_E-siliculosus-1a_M_contig75.15638.1 (polypeptide) Ectocarpus siliculosus Ec864m_EcPH12_78m male

BLAST of mRNA_E-siliculosus-1a_M_contig75.15638.1 vs. uniprot
Match: A0A6H5JTK0_9PHAE (Replication protein A subunit n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JTK0_9PHAE)

HSP 1 Score: 1181 bits (3054), Expect = 0.000e+0
Identity = 638/670 (95.22%), Postives = 647/670 (96.57%), Query Frame = 0

Query:    1 MANLISRGAINQIMGGATGAAGYNPTVQVVSLKKMTNAGTDRWRLVLSDGQHFAQSMLATQQNEMISSGDLKELCIITLSDYIVNVVQNKRLIILLKLDVTSGPAPKIGDPINFSEDSSTGMKQELGGGSNNN-TGHYXXXXXXXXXXXXXXRDVKPKVANNPYGASNKQAXXXYAGGGSNNNYXXXXXXXXXXXXARGGAANXPYANRGSSNSGPVQRQSQDSAYMPISAINPYQNRWTIKARITKKGDMRSWNNARGSGTLFSVDLLDEDGSEIKGTFFKQDADKWFQILEEGQVYAFTGGKVKVANKKFSSFNAEYELTFDSSTQVNPINDDSRIGSATYAFVKLNEMEAMEANKVLDVIAVVKSVEDHAQFVSSRAGKQLDKRNLILVDDTCTEINLTLWGDMAKADGSRWEGNPVVAFKGVKLSDFNGRSLNSLNGSTLVNDPDVPETADLRAWFDAAGGGSSFKSVTVXXXXXXXXXXAAKKDISQRYTLQSITDGNLGNGEKPDWAVVKATISFIKLDGERLPWYTACPKEGCNKKVTETMEGAWQCEKCNQTYPECQRRYMLSMQISDNTGKAWVTAFNDQGVELLGNRTADELFQMKEEGNDAELEEIFSEACFKTYMLTLRVKTDMYNDETRSRKTLQRLAPVDVKAECAALLDAIAKYN 669
            MANLISRGAINQIMGGATGAAGYNPTVQVVSLKKMTNAGTDRWRLVLSDGQHFAQSMLATQQNEMISSGDLKELCIITLSDYIVNVVQNKRLIILLKLDVTSGPAPKIGDPINFSEDSS  +K ELGGGSNNN TGHY XX   XXXXXXXXRDVKPKVANNPYGASNKQ XXX AGGG NNNYXXXXXXXXXXXXARGGAAN PYANRGSSNSGPVQRQSQDSAYMPISAINPYQNRWTIKARITKKGDMR WNNARGSGTLFSVDLLDEDGSEIKGTFFKQDADKWFQIL+EGQVYAFTGGKVKVANKKFSSFNAEYEL FDSSTQ+NPINDDSRI SATYAFVKLNEMEAMEANKVLDVIAVVKSVEDHAQFVS RAGKQLDKRNLILVDDTCTEINLTLWGDMAKADGSRWEGNPVVAFKGVKLS++NGRSLNSLN STLVNDPDVPETADLRAWFDAAGGGSSFKSVTVXXXXXXXX   AKKDISQRYTLQSITDGNLGNGEKPDWAVVKATISFIKLDGERLPWYTACPKEGCNKKVTETMEGAWQCEKCNQT+PECQRRYML+MQISDNTGKAWVTAFNDQ VELL NRTADELFQMKEEGNDAELEE+FSEACFKTYM TLRVKTDMYNDETRSRKTLQR+APVDVKAECAALLDAIAKYN
Sbjct:    1 MANLISRGAINQIMGGATGAAGYNPTVQVVSLKKMTNAGTDRWRLVLSDGQHFAQSMLATQQNEMISSGDLKELCIITLSDYIVNVVQNKRLIILLKLDVTSGPAPKIGDPINFSEDSSARVKPELGGGSNNNNTGHYGXXSKPXXXXXXXXRDVKPKVANNPYGASNKQXXXXXAGGG-NNNYXXXXXXXXXXXXARGGAANNPYANRGSSNSGPVQRQSQDSAYMPISAINPYQNRWTIKARITKKGDMRQWNNARGSGTLFSVDLLDEDGSEIKGTFFKQDADKWFQILQEGQVYAFTGGKVKVANKKFSSFNAEYELNFDSSTQINPINDDSRISSATYAFVKLNEMEAMEANKVLDVIAVVKSVEDHAQFVS-RAGKQLDKRNLILVDDTCTEINLTLWGDMAKADGSRWEGNPVVAFKGVKLSEYNGRSLNSLNSSTLVNDPDVPETADLRAWFDAAGGGSSFKSVTVXXXXXXXXDEVAKKDISQRYTLQSITDGNLGNGEKPDWAVVKATISFIKLDGERLPWYTACPKEGCNKKVTETMEGAWQCEKCNQTHPECQRRYMLNMQISDNTGKAWVTAFNDQAVELLDNRTADELFQMKEEGNDAELEELFSEACFKTYMFTLRVKTDMYNDETRSRKTLQRMAPVDVKAECAALLDAIAKYN 668          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15638.1 vs. uniprot
Match: D7FUU1_ECTSI (Replication protein A subunit n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FUU1_ECTSI)

HSP 1 Score: 802 bits (2071), Expect = 1.750e-288
Identity = 408/420 (97.14%), Postives = 414/420 (98.57%), Query Frame = 0

Query:  250 MRSWNNARGSGTLFSVDLLDEDGSEIKGTFFKQDADKWFQILEEGQVYAFTGGKVKVANKKFSSFNAEYELTFDSSTQVNPINDDSRIGSATYAFVKLNEMEAMEANKVLDVIAVVKSVEDHAQFVSSRAGKQLDKRNLILVDDTCTEINLTLWGDMAKADGSRWEGNPVVAFKGVKLSDFNGRSLNSLNGSTLVNDPDVPETADLRAWFDAAGGGSSFKSVTVXXXXXXXXXXAAKKDISQRYTLQSITDGNLGNGEKPDWAVVKATISFIKLDGERLPWYTACPKEGCNKKVTETMEGAWQCEKCNQTYPECQRRYMLSMQISDNTGKAWVTAFNDQGVELLGNRTADELFQMKEEGNDAELEEIFSEACFKTYMLTLRVKTDMYNDETRSRKTLQRLAPVDVKAECAALLDAIAKYN 669
            MR WNNARGSGTLFSVDLLDEDGSEIKGTFFKQDADKWFQIL+EGQVYAFTGGKVKVANKKFSSFNAEYELTFDSSTQ+NPINDDSRIGSATYAFVKLNEMEA+EANKVLDVIAVVKSVEDHAQFVS RAGKQLDKRNLILVDDTCTEINLTLWGDMAKADGSRWEGNPVVAFKGVKLSDF+GRSLNSLN STLVNDPDVPETADLRAWFDAAGGGSSFKSVT XXXXXXXXXX AKKDISQRYTLQSITDGNLGNGEKPDWAVVKATISFIKLDGERLPWYTACPKEGCNKKVTETMEGAWQCEKCNQT+PECQRRYMLSMQISDNTGKAWVTAFNDQGVELL NRTADELFQMKEEGNDAELEE+FSEACFKTYMLTLRVKTDMYNDETRSRKTLQRLAPVDVKAECAALLDAIAKYN
Sbjct:    1 MRQWNNARGSGTLFSVDLLDEDGSEIKGTFFKQDADKWFQILQEGQVYAFTGGKVKVANKKFSSFNAEYELTFDSSTQINPINDDSRIGSATYAFVKLNEMEALEANKVLDVIAVVKSVEDHAQFVS-RAGKQLDKRNLILVDDTCTEINLTLWGDMAKADGSRWEGNPVVAFKGVKLSDFSGRSLNSLNASTLVNDPDVPETADLRAWFDAAGGGSSFKSVTXXXXXXXXXXXXAKKDISQRYTLQSITDGNLGNGEKPDWAVVKATISFIKLDGERLPWYTACPKEGCNKKVTETMEGAWQCEKCNQTHPECQRRYMLSMQISDNTGKAWVTAFNDQGVELLDNRTADELFQMKEEGNDAELEELFSEACFKTYMLTLRVKTDMYNDETRSRKTLQRLAPVDVKAECAALLDAIAKYN 419          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15638.1 vs. uniprot
Match: A0A7S3XQ94_HETAK (Replication protein A subunit n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XQ94_HETAK)

HSP 1 Score: 475 bits (1222), Expect = 1.480e-156
Identity = 289/669 (43.20%), Postives = 427/669 (63.83%), Query Frame = 0

Query:    1 MANLISRGAINQIMGGATGAAGYNPTVQVVSLKKMTNAGTDRWRLVLSDGQHFAQSMLATQQNEMISSGDLKELCIITLSDYIVNVVQNKRLIILLKLDVTSGPAPKIGDPINFSEDSSTGMKQELGGGSNNNTGHYXXXXXXXXXXXXXXRDVKPKV--ANNPYGASNKQAXXXYAGGGSNNNYXXXXXXXXXXXXARGGAANXPYANRGSSNSGPVQRQSQDSAYMPISAINPYQNRWTIKARITKKGDMRSWNNARGSGTLFSVDLLDEDGSEIKGTFFKQDADKWFQILEEGQVYAFTGGKVKVANKKFSSFNAEYELTFDSSTQVNPINDDSRIGSATYAFVKLNEMEAMEANKVLDVIAVVKSVEDHAQFVSSR-AGKQLDKRNLILVDDTCTEINLTLWGDMAKADGSRWEGNPVVAFKGVKLSDFNGRSLNSLNGSTLVNDPDVPETADLRAWFDAAGGGSSFKSVTVXXXXXXXXXXAAKKDISQRYTLQSITD-GNLGNGEKPDWAVVKATISFIKLDGERLPWYTACPKEGCNKKVTETMEGAWQCEKCNQTYPECQRRYMLSMQISDNTGKAWVTAFNDQGVELLGNRTADELFQMKEEGNDAELEEIFSEACFKTYMLTLRVKTDMYNDETRSRKTLQRLAPVDVKAECAALLDAI 665
            MANL S G+I QI+ G     G  PT+QV+++KK+  AG DR+RL++SDGQ++AQ MLATQ N ++SS +L+ELCI+ L++Y+VN VQ +R++I L ++V SGP PK+GDP N S   +  M  +            XXX      XXXXX         ++NPYG  +  +XXX   G +N N XXXXXXX                   ++++GPV R++ ++ Y PI+++NPYQNRWTIKAR+TK+ D+R W+NARG G L SVDLLD +G+EI+ T FK+D D+ ++ L+EG+V++F+GG++KVANK+++S   +YE+TF S T V    D++ I    ++FV +  +   +A  V D+IAVVKS  + +  +S +  GK+L KR+L LVD +  E+ LT+WG+ A A  ++WE +PVVAFKG++ SD+ G SL+SLN + ++ +PD+PE   L AW+   GG +  ++ ++            K  +  R  L  I D   +G GEKPD+A VKA +S+++ D    PWYT+CP + C KK+TE  +  W CEKCN+ Y  C+ RY+LS +  + +G+ WV+AFND+  E++ N+TADEL Q++ EGND   E  F+ A F+ ++  LR+K + YND+ R + +L RL PVD   E   LL+AI
Sbjct:    1 MANLTS-GSIQQIVNGEV--TGLTPTLQVINVKKVQGAGADRFRLIISDGQYYAQCMLATQLNHLVSSNELQELCIVKLTNYVVNNVQGRRIVIGLAVEVVSGPVPKVGDPQNSSGGPAPAMPAQXXXXXXXXXXXXXXXPYQQGSXXXXXXXXXXXXXXSSNPYGGGSSNSXXXX--GNTNTNTXXXXXXX-------------------TTSTGPVARENPNAQYQPIASLNPYQNRWTIKARVTKRSDIRRWSNARGEGHLASVDLLDAEGTEIRATMFKEDCDRLYERLQEGKVFSFSGGRLKVANKQYTSIKNDYEITFGSETVVEGPLDEAGIEQNKFSFVDIAALSP-DAGMV-DLIAVVKSAGELSTIISKKQGGKELQKRDLTLVDRSGAEVRLTVWGESALAQ-AQWEHSPVVAFKGLRASDYGGVSLSSLNSTVVMINPDLPEGHALHAWWTQQGGATQ-QTRSLSAAGPGGSRWVTK--VQNRAQLGVIKDIPGIGAGEKPDYAEVKAMLSYMRHDNG--PWYTSCPNDDCKKKMTEGFDQKWSCEKCNKQYDRCKHRYVLSTRFIEASGQDWVSAFNDE-AEMILNKTADELQQLQSEGNDVAYEAAFAAANFQHFVAQLRIKQETYNDQARLKLSLIRLKPVDPVEESRMLLEAI 636          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15638.1 vs. uniprot
Match: A0A835Z8X4_9STRA (Replication protein A subunit n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z8X4_9STRA)

HSP 1 Score: 451 bits (1161), Expect = 2.580e-147
Identity = 287/666 (43.09%), Postives = 401/666 (60.21%), Query Frame = 0

Query:    5 ISRGAINQIMGGATGAAGYNPTVQVVSLKKMTNAGT--DRWRLVLSDGQHFAQSMLATQQNEMISSGDLKELCIITLSDYIVNVVQNKRLIILLKLDVTSGPAPKIGDPINFSEDSSTGMKQELGGGSNNNTGHYXXXXXXXXXXXXXXRDVKPKVANNPYGASNKQAXXXYAGGGSNNNYXXXXXXXXXXXXARGGAANXPYANRGSSNSGPVQRQSQDSAYMPISAINPYQNRWTIKARITKKGDMRSWNNARGSGTLFSVDLLDEDGSEIKGTFFKQDADKWFQILEEGQVYAFTGGKVKVANKKFSSFNAEYELTFDSSTQVNPINDDSRIGSATYAFVKLNEMEAMEANKVLDVIAVVKSVEDHAQFVSSRAGKQLDKRNLILVDDTCTEINLTLWGDMAKADGSRWEGNPVVAFKGVKLSDFNGRSLNSLNGSTLVNDPDVPETADLRAWFDAAGGGSSFKSVTVXXXXXXXXXXAAKKDISQRYTLQSITDGNLGNGEKPDWAVVKATISFIKLDGERLPWYTACPKEGCNKKVTETMEGAWQCEKCNQTYPECQRRYMLSMQISDNTGKAWVTAFNDQGVELLGNRTADELFQMKEEGNDAELEEIFSEACFKTYMLTLRVKTDMYNDETRSRKTLQRLAPVDVKAECAALLDAIAKY 668
            +++GAI +I+  A+G  G+ P VQV+S+KKMT A    +R+R+V+SDGQH  QSML T QN++I+SG + E C+I L ++I+N +  K ++I+L+  V SG  PKIGDP  F               ++      XXXXXXXXXXX       P  A +    S++        G +    XXXXXXXXXXXX         Y + G + SGPVQR    ++Y PIS +NPY NRWTIKAR+T K ++R W+N RG G L SVDLLDE G E++GTFFK   DK+  +LE   VY F+GG++KVANK++SS   +YE+TF+S + +  + DD RI +    F ++ +++ +E N  +D++AVV+  +D A  + ++  ++++KRN+ LVDD+  E+  TLWG  AK       G+PV A                   ST+V  PD+ E   L  W+    G         XXXXXXXX  +   D++QR  +  I +  LG  EKPD+A +KA ++FIK D E  PWYTACP EGCNKKV E M+  W+CEKC+++YP C+RRY+ S+ +SD+TG+ W +AFND    +L   +ADELF+ K  G D   E+ F EAC+K Y+L +RVK +M NDE R + T+    PVD   E AAL+ AI  Y
Sbjct:    6 VTQGAIQRIIT-ASGQDGFLPIVQVLSIKKMTAAAGQGERYRVVVSDGQHHQQSMLTTSQNDLITSGQMVEGCLICLDEFILNQIGAKSIVIILRASVLSGAMPKIGDPTAFER-------------ADGXXXXXXXXXXXXXXXXTRATATAPLAALSSRWRSSR--------GRAXXXXXXXXXXXXXXXXXXXXXXXGGYGHSGGA-SGPVQRHDSGASYTPISDLNPYMNRWTIKARVTAKSEIRRWSNQRGEGYLASVDLLDEFGGEVRGTFFKDAVDKYLPMLEVNSVYTFSGGRIKVANKQYSSLQNDYEITFNSDSVIEKVADDKRIKAMNLTFRRIADLKDVEVNATIDILAVVRDFQDMA-MIMTKNNREVEKRNITLVDDSGAEVVCTLWGAHAKLPDDALRGSPVAAXXXXXXXXXXXXXXXXXXSSTVVLAPDLEEARALHNWWTRYAG-XXXXXXXXXXXXXXXXPRSDPYDVAQRLGVAGIKEEQLGLKEKPDYASLKAYVAFIKHDSENGPWYTACPTEGCNKKVVEEMDQGWRCEKCSRSYPNCRRRYIASLAMSDHTGQTWFSAFNDVAQPMLAQHSADELFEWKTSGQDELFEDTFVEACWKQYVLRVRVKAEMVNDEQRIKSTIVDAEPVDPVKESAALIRAIKAY 646          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15638.1 vs. uniprot
Match: A0A6U0TYW6_9STRA (Replication protein A subunit n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A6U0TYW6_9STRA)

HSP 1 Score: 437 bits (1125), Expect = 6.320e-142
Identity = 266/676 (39.35%), Postives = 386/676 (57.10%), Query Frame = 0

Query:    5 ISRGAINQIMGGATGAAGYNPTVQVVSLKKMTNAG--TDRWRLVLSDGQHFAQSMLATQQNEMISSGDLKELCIITLSDYIVNVVQNKRLIILLKLDVTSGPAPKIGDPINFSEDSSTGMKQELGGGSNNNTGHYXXXXXXXXXXXXXXRDVKPKVANNPYGASNKQAXXXYAGGGSNNNYXXXXXXXXXXXXARGG------AANXPYANRGSS---NSGPVQRQSQDSAYMPISAINPYQNRWTIKARITKKGDMRSWNNARGSGTLFSVDLLDEDGSEIKGTFFKQDADKWFQILEEGQVYAFTGGKVKVANKKFSSFNAEYELTFDSSTQVNPINDDSRIGSATYAFVKLNEMEAMEANKVLDVIAVVKSVEDHAQFVSSRAGKQLDKRNLILVDDTCTEINLTLWGDMAKADGSRWEGNPVVAFKGVKLSDFNGRSLNSLNGSTLVNDP-DVPETADLRAWFDAAGGGSSFKSVTVXXXXXXXXXXAAKKDISQRYTLQSITDGNLGNGEKPDWAVVKATISFIKLDGERLPWYTACPKEGCNKKVTETMEGAWQCEKCNQTYPECQRRYMLSMQISDNTGKAWVTAFNDQGVELLGNRTADELFQMKEEGNDAELEEIFSEACFKTYMLTLRVKTDMYNDETRSRKTLQRLAPVDVKAECAALLDAIAKY 668
            + RG + +I     G     P +QV+ ++K++NA   ++R+R+VLSDG+++  +MLATQQN +++SG LK   ++ L  Y+VN VQ KR++IL+  DV  G A +I            G  Q+L  G++                        P     P        XXX          XXXXXXXXX     GG      A    Y    SS   NS PV R   D   MPI+A+N Y ++WTIKAR+T K  +R W+NARG G LF+VDLLD  G EI+ TFFK   D++  +L+EG+VY F+GG++K+A K+++S   +YE+TFD +  + P +D+  I    Y F K+ ++E +     +D++AVVKS  +    +S +  K+L KR+L+LVDD+ TEI +TLWGD A+ DG +W  NPV+A KGVK+SD+NGRSL+ L  S    D  D PE   LR W+ A  G     S++           AA   ++QR  L +I D  LG   + ++  VK T++ ++ +G   PWYTA P+EG   KVTET EG W CE+  +TY  C+RR++LS+ ++D +G  WVTAFND    LLG +TADE ++M+  G++   +E FS+  FK  +  LRVKT+  NDE R + ++  +  V+ K EC  L++ IAK+
Sbjct:    3 LDRGILRRI---DNGEMDLKPVLQVIHIRKISNANGPSERFRIVLSDGENYNTAMLATQQNGVVNSGALKVHSLVRLGYYMVNEVQGKRILILINFDVV-GEAERI-----------LGKPQDLHSGAS------------ADKFDKNAAAGAPPARPQPQQXXXXXXXXXXXXXXXXXXXXXXXXXXXXSNAMPGGGEPARTAPKPEYGTYSSSMMGNSAPVARVESDDRIMPIAALNLYNSKWTIKARVTSKSAIRRWSNARGEGHLFNVDLLDGQGGEIRATFFKDACDRFEPLLQEGRVYTFSGGRLKLAKKEYTSIKNDYEITFDRNADIRPSDDEGGIKRIKYNFTKIGDIEKVAPGATIDLVAVVKSCGECNSIMSQKLSKELFKRDLVLVDDSNTEIQMTLWGDRAREDGGQWMSNPVIAVKGVKVSDYNGRSLSLLQSSNFSTDTTDSPEAGRLRQWWSANQGNVQTTSISAKSGGGG----AAGTSVAQRQPLSTIKDEGLGTKGETEYLAVKGTVTLLQTNGNNDPWYTAAPEEGVLFKVTETTEGQWFCERNGKTYDTCKRRFVLSLVLTDYSGNTWVTAFNDPAELLLGGKTADECYEMRARGDEQGYKECFSKGQFKQCIFKLRVKTEQLNDEQRVKTSIVTIRDVNYKEECRQLIEEIAKF 647          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15638.1 vs. uniprot
Match: A0A317XXN4_9BASI (Replication protein A subunit n=1 Tax=Testicularia cyperi TaxID=1882483 RepID=A0A317XXN4_9BASI)

HSP 1 Score: 422 bits (1085), Expect = 3.310e-136
Identity = 252/684 (36.84%), Postives = 370/684 (54.09%), Query Frame = 0

Query:    5 ISRGAINQ-IMGGATGAAGYNPTVQVVSLKKMTNAGT------DRWRLVLSDGQHFAQSMLATQQNEMISSGDLKELCIITLSDYIVNVVQNKRLIILLKLDVTSGPAP-KIGDPINFSEDSSTG-----MKQELGGGSNNNTGHYXXXXXXXXXXXXXXRDVKPKVANNPYGASNKQAXXXYAGGGSNNNYXXXXXXXXXXXXARGGAANXPYANRGSSNSGPVQRQSQDSAYM-----PISAINPYQNRWTIKARITKKGDMRSWNNARGSGTLFSVDLLDEDGSEIKGTFFKQDADKWFQILEEGQVYAFTGGKVKVANKKFSSFNAEYELTFDSSTQVNPINDDSRIGSATYAFVKLNEMEAMEANKVLDVIAVVKSVEDHAQFVSSRAGKQLDKRNLILVDDTCTEINLTLWGDMAKA--DGSRWEGNPVVAFKGVKLSDFNGRSLNSLNGSTLVNDPDVPETADLRAWFDAAGGGSSFKSVTVXXXXXXXXXXAAKKDISQRYTLQSITDGNLGNGEKPDWAVVKATISFIKLDGERLPWYTACPKEGCNKKVTETMEGAWQCEKCNQTYPECQRRYMLSMQISDNTGKAWVTAFNDQGVELLGNRTADELFQMKEEGNDAELEEIFSEACFKTYMLTLRVKTDMYNDETRSRKTLQRLAPVDVKAECAALLDAIAKY 668
            ++ GAI Q I    T     NP  Q++S+KK+    T      DR+R++LSDG H+AQ+MLA+Q+  M+ SG+L++ CI+ +  Y  N VQN+R++ILL L V SGPAP ++G+PIN  E    G     +KQE G                            P  A     A+ +      A GG                         P A           R    S +      PI  ++PYQNRWTIKAR+T K D+R W+N RG G LFSV+LLDE G EIK T F    D+++ +L+E  VY  +  +V +A K+FS+   EYE+TF++ T++    D S +    Y FV++NE+E++E N+  DVI V+   ++ ++ VS  + + + KR L LVD +   + LTLWG  A+   + +  +  PV+AFKGVK+ DF GRSL+  + ST+  +PD+ E   LR WFD  G  S FK  T               ++++R T+  + D N+G  EKPD+  V+AT+ +IK D     +YTACP +GCNKKVT+ M+G W+CEKC+++YP  + RY+LS  ++D++G+ W++ FND   +L+G  TA EL Q++EE ++ E       A  + Y+   R K D +ND  R R T+ R  PVD       L++AI  Y
Sbjct:    6 LTHGAIAQMIQSSDTSNLVQNPVCQILSIKKIQATSTTAANVGDRYRIILSDGVHYAQAMLASQKRTMVESGELEKNCIVKVLQYAANSVQNRRILILLDLQVLSGPAPDRLGNPINVDEAQKAGGEAGPVKQESGA---------------------------PAAA----AAAGRTTPATSAFGGK------------------------PAAXXXXXXXXXXXRPGGSSIHAGMPIYPIEGLSPYQNRWTIKARVTSKSDIRHWSNQRGDGKLFSVNLLDESG-EIKATGFNDAVDRFYPLLQENHVYLISKARVNIAKKQFSNLTNEYEITFENQTEIEECTDASDVPEVKYEFVRINELESVEPNQTCDVIGVLDKYDELSEIVSKASQRPVQKRELSLVDQSGRSVRLTLWGKNAETFPNNAGVDEKPVIAFKGVKVGDFGGRSLSMFSSSTMTINPDIAEAHMLRGWFDNDGAHSEFKPYT--NAGGSGGAAGGTGNLAERRTIAQVKDENMGMSEKPDYFNVRATVVYIKQDNL---YYTACPSDGCNKKVTQDMDGNWRCEKCDRSYPAPEYRYILSTNVADSSGQMWLSGFNDDATKLIG-MTAGELHQLREE-SETEFAAALHRAANRMYVFNCRAKMDTFNDTARVRYTISRANPVDFAKAGLELVEAIRAY 626          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15638.1 vs. uniprot
Match: W7T7F5_9STRA (Replication protein A subunit n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7T7F5_9STRA)

HSP 1 Score: 423 bits (1088), Expect = 5.370e-136
Identity = 251/656 (38.26%), Postives = 374/656 (57.01%), Query Frame = 0

Query:   19 GAAGYNPTVQVVSLKKMT----NAGTDRWRLVLSDGQHFAQSMLATQQNEMISSGDLKELCIITLSDYIVNVVQNKRLIILLKLDVTSGPAP-KIGDPINFSEDSSTGMKQELGGGSNNNTGHYXXXXXXXXXXXXXXRDVKPKVANNPYGASNKQAXXXYAGGGSNNNYXXXXXXXXXXXXARGGAANXPYANRGSSNSGPVQRQSQDSAYMPISAINPYQNRWTIKARITKKGDMRSWNNARGSGTLFSVDLLDEDGSEIKGTFFKQDADKWFQILEEGQVYAFTGGKVKVANKKFSSFNAEYELTFDSSTQVNPINDDSRIGSATYAFVKLNEMEAMEANKVLDVIAVVKSVEDHAQFVSSRAGKQLDKRNLILVDDTCTEINLTLWGDMAKADGSRWEGNPVVAFKGVKLSDFNGRSLNSLNGSTLVNDPDVPETADLRAWFDAAGGGSSFKSVTVXXXXXXXXXXAAKKDISQRYTLQSITDGNLGNGEKPDWAVVKATISFIKLDGERLPWYTACPKEGCNKKVTETMEGAWQCEKCNQTYPECQRRYMLSMQISDNTGKAWVTAFNDQGVELLGNRTADELFQMKEEGNDAELEEIFSEACFKTYMLTLRVKTDMYNDETRSRKTLQRLAPVDVKAECAALLDAIAKYN 669
            G   ++P VQV+  K++       G  R+RL++SDG H+ Q+MLATQ N +I+ G + E  +I L ++I N VQ ++++I+L+  V S     ++GDP+ + +  + G    + GG     G                   KP   ++  G+SN  A   Y  GG+   Y                             SGPV R     + +PI+ +NPY NRW IKAR+T K D++ W+NA+G GTLFSVDLLDE   EI+ TFFK+  +K++ +LE+G VY F+GGK+K A +++SS    YE TFD+++++  + DD +I SA Y+F K+ E+E    N  +DVI VVK+    +   + + G++++KR L L DDT TE+ LT+WG+ A     +WE +P+VAFKGVK+SD+NGRSL ++ GS +   PD+PE   L  W+ +AG      +   XXXXXXXXXX      + R  L  I +  LG GEKPD+  VKAT+++IK D +  PWY +C    C +KVTE +   + CEKC +  PEC  RY+L++  +D++G  +++ F++    LLG R+A+EL   K     A  +  F EA F T++L LRVK +  NDE R + T+  + PV++  E   L+ AI  YN
Sbjct:   62 GQTKFSPVVQVMDFKQVQPNHGQPGQVRYRLIVSDGTHYQQAMLATQLNVLINEGQITEHGLICLEEFICNEVQGRKIVIILRCHVVSPSLGYRVGDPVEYGK--AAGGNGGMQGGQPV-YGQQPQQAPALNTMAGASVPYKP-AGSSSAGSSNAGAYPPYNSGGAAGGYRAP--------------------------SGPVVRAGPPQSIIPINDLNPYINRWVIKARVTSKTDIKRWSNAKGEGTLFSVDLLDEFDGEIRATFFKEACEKFYPLLEQGHVYTFSGGKLKPAQRQYSSIRNNYETTFDTNSEIMEVMDDHKIQSAVYSFKKIGELEETPPNATIDVIGVVKAFSPLSTITTKKDGREVEKRELTLCDDTGTEVRLTVWGERASKQPEQWENHPIVAFKGVKVSDYNGRSLGTMPGSQVTVSPDLPEALHLHNWWHSAGA-----TXXXXXXXXXXXXXXXXXXFADRKVLSQIKEEQLGFGEKPDYMDVKATLTYIKSDPD--PWYRSCTSPDCKRKVTEQLNSYF-CEKCQREMPECAYRYILTVSGTDHSGSVYLSCFDEHANILLG-RSAEELNSFKASDMQARYDATFQEATFGTHILRLRVKAEQVNDEQRVKSTIMNIRPVNLVQEIRDLITAIKAYN 678          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15638.1 vs. uniprot
Match: I2FRR5_USTH4 (Replication protein A subunit n=1 Tax=Ustilago hordei (strain Uh4875-4) TaxID=1128400 RepID=I2FRR5_USTH4)

HSP 1 Score: 421 bits (1082), Expect = 9.300e-136
Identity = 255/680 (37.50%), Postives = 387/680 (56.91%), Query Frame = 0

Query:    3 NLISRGAINQIMGGATGAAGY-NPTVQVVSLKKMTNAGT------DRWRLVLSDGQHFAQSMLATQQNEMISSGDLKELCIITLSDYIVNVVQNKRLIILLKLDVTSGP-APKIGDPINFSE--DSSTGMKQELGGGSNNNTGHYXXXXXXXXXXXXXXRDVKPKVANNPYGASNKQAXXXYAGGGSNNNYXXXXXXXXXXXXARGGAANXPYANRGSSNSG-PVQRQSQDSAYMPISAINPYQNRWTIKARITKKGDMRSWNNARGSGTLFSVDLLDEDGSEIKGTFFKQDADKWFQILEEGQVYAFTGGKVKVANKKFSSFNAEYELTFDSSTQVNPINDDSRIGSATYAFVKLNEMEAMEANKVLDVIAVVKSVEDHAQFVSSRAGKQLDKRNLILVDDTCTEINLTLWGDMAKA--DGSRWEGNPVVAFKGVKLSDFNGRSLNSLNGSTLVNDPDVPETADLRAWFDAAGGGSSFKSVTVXXXXXXXXXXA-AKKDISQRYTLQSITDGNLGNGEKPDWAVVKATISFIKLDGERLPWYTACPKEGCNKKVTETMEGAWQCEKCNQTYPECQRRYMLSMQISDNTGKAWVTAFNDQGVELLGNRTADELFQMKEEGNDAELEEIFSEACFKTYMLTLRVKTDMYNDETRSRKTLQRLAPVDVKAECAALLDAIAKY 668
            N +S+GAI Q++  A  A    NP  Q++S+KK+  + T      DR+R++LSDG H+AQSMLA+Q+  M+ SG+L++ C++ +S Y  N VQN+R++ILL L+V   P A ++G P N  +   S  G+KQE  GG+ N  G+                     + NN  G +        AG                     GG A  P  +  S N+G P+          PI  ++PYQNRWTIKAR+T K D+R W+N RG G LFSV+LLD+ G EIK T F    D+++ +L+E  VY  +  KV +A K+FS+   EYE+TF++ST++    D + +    Y FV++NE+E++E N+  DVI V+ S  + ++ VS  + + + KR L LVD     + LTLWG  A+   + +  +  PV+AFKGVK+ DF GRSL+  + ST++ +PD+ E+  LR W+D  G  + F++ +           A A  ++++R T+  + D NLG  EKPD+  ++AT+ +IK +     +YTACP +GCNKKVT   E  W+CEKC+++Y   + RY+LS  ++D TG+ W++ FN+   +L+G  +A EL Q++E  +++E       A  + Y+   R K D +ND+TR R T+ R APVD     A L+DAI  Y
Sbjct:    4 NDLSQGAIAQMIQTADPANSVQNPICQILSIKKIQASATSASNVGDRYRIILSDGVHYAQSMLASQKRSMVESGELEKHCLVRISQYASNSVQNRRILILLDLEVAHKPTADRLGSPTNVDDAIKSEGGVKQE--GGAGNALGN-------------------SNLGNNSAGRTTPATSAAGAG-------------------KIGGGAGRPGGS--SVNAGMPIY---------PIEGLSPYQNRWTIKARVTSKSDIRHWSNQRGEGKLFSVNLLDDSG-EIKATGFNDTVDRFYSLLKENHVYLISKAKVNIAKKQFSNLQNEYEITFENSTEIEECTDATDVPEVKYEFVRINELESVEPNQTCDVIGVLDSYGELSEIVSKASQRPVQKRELTLVDQGGKSVRLTLWGKNAETFPNNAGVDEKPVIAFKGVKVGDFGGRSLSMFSSSTMLINPDITESHVLRGWYDNDGARAQFEAFSNAGVGGGALGAAGAGGNMAERRTIAQVKDENLGMTEKPDYFNIRATVVYIKQENL---YYTACPSDGCNKKVTLDHENNWRCEKCDRSYEAPEYRYILSTNVADATGQIWLSGFNEDATKLIG-MSAGELHQLRE-NSESEYSAALHRAANRMYLFNCRAKMDTFNDQTRVRYTISRSAPVDFAKAGAELVDAIKVY 626          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15638.1 vs. uniprot
Match: A0A7S2JN56_9EUKA (Replication protein A subunit n=1 Tax=Cyanoptyche gloeocystis TaxID=77922 RepID=A0A7S2JN56_9EUKA)

HSP 1 Score: 413 bits (1062), Expect = 3.210e-132
Identity = 252/662 (38.07%), Postives = 369/662 (55.74%), Query Frame = 0

Query:   19 GAAGYNPTVQVVSLKKMTNAGT---DRWRLVLSDGQHFAQSMLATQQNEMISSGDLKELCIITLSDYIVNVVQNKRLIILLKLDVTSGPAPKIGDPINFSEDSSTGMKQELGGGSNNNTGHYXXXXXXXXXXXXXXRDVKPKVANNPYGASNKQAXXXYAGGGSNNNYXXXXXXXXXXXXARGGAANXPYANRGSSNSGPVQRQSQDSAY----MPISAINPYQNRWTIKARITKKGDMRSWNNARGSGTLFSVDLLDEDGSEIKGTFFKQDADKWFQILEEGQVYAFTGGKVKVANKKFS-SFNAEYELTFDSSTQVNPINDDSRIGSATYAFVKLNEMEAMEANKVLDVIAVVKSVEDHAQFVSSRAGKQLDKRNLILVDDTCTEINLTLWGDMAKADGSRWEG------NPVVAFKGVKLSDFNGRSLNSLNGSTLVNDPD-VPETADLRAWFDAAGGGSSFKSVTVXXXXXXXXXXAAKKDISQRYTLQSITDGNLGNGEKPDWAVVKATISFIKLDGERLPWYTACPKEGCNKKVTETMEGAWQCEKCNQTYPECQRRYMLSMQISDNTGKAWVTAFNDQGVELLGNRTADELFQMKEEGNDAELEEIFSEACFKTYMLTLRVKTDMYNDETRSRKTLQRLAPVDVKAECAALLDAI 665
            G A   P +QV+ +K++ N G    DR+RL LSDG  F QSMLATQ N+++++G+L++ C++ L+++I NVVQN+++II L + V SGP P IG+P        T     + GG  NN G Y              + V                XXX     S+NN                 A    Y+    SN G    +++ S       PISA+NPY NRWTIKAR+T KGD+++++NARGSG + +V  LDE+G EI+ T F    DK   + E   VY  + G +K ANK+FS     EYE+T D    V  I+DD  IGS  ++FVK++++E +  N+V+DVI VV  +   ++  +   G QL +RNL L DD+   + LTLWGD  KA+  +W+        P++A + V++SDFNGRSL+S + S L  DPD + E   LR WFD+        SV+             +   ++R  L +I D +LGN +K D    KAT+ FIK D     WY +CP + C KKVT      W+CEKC+++Y   + RYMLS+ ++D+T   WV+AF D G ++LG  TAD+L  +KE  N AE + +FSEA FK+Y+  ++ K + YNDE+R + ++  + P+D ++E   +LD+I
Sbjct:   20 GHAEKQPLLQVLDVKRIGNPGAPQGDRYRLQLSDGVFFTQSMLATQLNDLVANGELQQHCVVQLTEHICNVVQNRKIIIALGMIVKSGPVPVIGNPRQLESPPIT--SDGVTGG--NNPGQYQQNNPKSSFQQSSYQSVPGNQNQXXXXXXXXXXXXXXXXXXSHNNPSNRQMPNSAPQQRNAAAVAPSYSGGMQSNRGASIAKAEGSLQGIPLTPISALNPYNNRWTIKARVTNKGDIKTYSNARGSGKILNVVFLDEEGGEIRATMFNDTVDKLNPVFELNGVYLVSKGTLKPANKQFSRGIKHEYEMTLDDRATVERISDDPSIGSMKWSFVKISQIEQINNNEVVDVIGVVIDIGHRSEITTKSKGNQLSRRNLKLADDSDCSVELTLWGD--KAEDQQWDDFMAQKHTPILACRHVRVSDFNGRSLSSTSDSGLFVDPDNIAEAGSLRFWFDSRNPTQHLSSVS--------SAGGGRGGQAERKLLSAIKDEDLGNKDKADVFSAKATVVFIKHDSAC--WYPSCPDDSCKKKVTGDENNGWRCEKCDKSYDRPKYRYMLSLSLNDSTAGCWVSAFGDDGTKILG-LTADDLQALKE-NNPAEYDRVFSEALFKSYIFKIKAKMETYNDESRVKLSVLAIDPMDFRSETTRMLDSI 663          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15638.1 vs. uniprot
Match: A0A7S2ULH8_9STRA (Replication protein A subunit n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2ULH8_9STRA)

HSP 1 Score: 409 bits (1052), Expect = 4.520e-131
Identity = 248/675 (36.74%), Postives = 389/675 (57.63%), Query Frame = 0

Query:    5 ISRGAINQIMGGATGAAGYNPTVQVVSLKKMTNAG-TDRWRLVLSDGQHFAQSMLATQQNEMISSGDLKELCIITLSDYIVNVVQNKRLIILLKLDV-TSGPAPKIGDPINFSEDSSTGMKQELGGGSNNNTGHYXXXXXXXXXXXXXXRDVKPKVANNPYGASNKQAXXXYAGGGSNNNYXXXXXXXXXXXXARGGAANXPYANRGSSNSGPVQRQSQDSA---YMPISAINPYQNRWTIKARITKKGDMRSWNNARGSGTLFSVDLLDEDGSEIKGTFFKQDADKWFQILEEGQVYAFTGGKVKVANKKFSSFNAEYELTFDSSTQVNPINDDSRIGSATYAFVKLNEMEAMEANKVLDVIAVVKSVEDHAQFVSSRAGKQLDKRNLILVDDTCTEINLTLWGDMAKADGSRWEGNPVVAFKGVKLSDFNGRSLNSLNGSTLVNDPDVPETADLRAWFDAAGGGSSFKSVTVXXXXXXXXXXAAKKDISQRYTLQSITDGNLG-NGEKPDWAVVKATISFIKLDGERLPWYTACPKEG--CNK--KVTETMEGAWQCEKCNQTYPECQRRYMLSMQISDNTGKAWVTAFNDQGVELLGNRTADELFQMKEEGNDAEL-EEIFSEACFKTYMLTLRVKTDMYNDETRSRKTLQRLAPVDVKAECAALLDAIAKY 668
            ++ GA+  +         Y P +QV+ +K +   G ++R+R + SDGQ+F Q MLATQ N ++ SG+++E  +I + +++ NVVQ + +II+LKL+V T+ P  + GDP +  +++   ++Q  GG +      Y                 +     N YGA +K       GG S    XXXXXXXXXXXXA                  P+ R S  ++   Y PIS +N YQNRWTIK R+T K  +R+W+NA+G G+LFS+D+LD   ++++ TFFK+  DK+F  LE  +VY FTGG++K AN ++++  + +E+TFD + +++  ND   I   T+ FV++ ++E +E  K +D++AVVKSV +    VS ++G++L K  L+LVDD+  E+ LT+WG+ A    + +  +P+V FK  ++SDF GRSL++   S +VN P +PET  +RAW+ + G  S+  + ++           A     +R T+ SI + ++G N +KPDW   K T +FIK D +   WY AC   G  C    KVT+T +G W C+KC  TYP C RR++ S  I+D+T   WV+ FN+Q   LLG  TAD++++   E +D +  +  FS+A F  ++   +V+ +  NDE R +  +Q L P+D   E   LL AIA++
Sbjct:    3 LTHGAVRTLATADQADPSYQPVLQVIHVKAVGGGGASERFRAIFSDGQNFVQGMLATQLNPLVHSGEIQENSVIAVKEFMKNVVQGRTVIIVLKLEVITANPGQRFGDPKDIEKEN---IQQSSGGAAPAAQPMYGNRPTPVESTTSGGMYNQSTQPTNTYGAGSK-------GGSSGAXXXXXXXXXXXXXXA------------------PIVRSSNPASGDNYTPISQLNIYQNRWTIKGRVTSKSAIRTWSNAKGDGSLFSIDILDASATDVRATFFKEAVDKFFNTLEVDKVYTFTGGRLKTANIQYNTCKSGFEITFDQNAEIHLDNDTGEIQQQTFEFVQIAQLEQVEPGKYVDILAVVKSVGEVGSIVSKKSGQELKKCELVLVDDSAVEVTLTVWGEEATKAQALYGNSPIVGFKRSRVSDFGGRSLSA--NSAVVN-PRLPETERVRAWWQSVGSNSANPTRSLSSAGGKGGRLPA---FDERKTVSSIKNEHMGQNTDKPDWVSFKGTFNFIKKDKDGGAWYPACANAGEPCKNRFKVTQTADGNWYCDKCQGTYPNCVRRFIFSATITDDTSTTWVSIFNEQAEVLLGGVTADDVYKNTHENHDQDAYDAFFSKATFTDWVFNCKVRQEFMNDEARVKTQIQSLHPMDYVQESRNLLQAIAQF 643