prot_E-siliculosus-1a_M_contig75.15628.1 (polypeptide) Ectocarpus siliculosus Ec864m_EcPH12_78m male

BLAST of mRNA_E-siliculosus-1a_M_contig75.15628.1 vs. uniprot
Match: D7FUT0_ECTSI (Methionine synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FUT0_ECTSI)

HSP 1 Score: 2354 bits (6100), Expect = 0.000e+0
Identity = 1192/1235 (96.52%), Postives = 1203/1235 (97.41%), Query Frame = 0

Query:    1 MLSITQPHIIKEIHTKYLESGSDIIGTNTFSATTIAMADYEMEKDVYELNFESARLAKEACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGGSDIIIVETIFDTLNAKAAVFAVREYFDEQGITDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLAQVAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFAQEGLINMLGGCCGSTPPHIKARRKGRAEKKIKRAMKDAVEPCSTRALPPPREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEKEKALKNKGKGTVEASGWRTQGVQARLTHSLIKGIDQWVIEDVEECRLMLDEQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQQVIKSARVMKKAVAHLLPFMEAEKLAKMVADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASELASATRFAVYDMGVMQPCDLILAKAKELNVDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLSDRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRKGIDFEKRPPVPPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLVGIFPAATVDGEDVEVYANDSREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADHMGMFAIACMGCEKMVKEFEELNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYDRD 1235
            MLSITQPHIIKEIHTKY++SGSDII TNTFSATTIAMADYEMEKDVYELNFESARLA+EACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGG+DIIIVETIFDTLNAKA VFAVREYFDEQGITDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLA+VAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFA+EGLINMLGGCCGSTP HIKA             MKDAVEPCSTRALPPPREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEKEKALKNKGKGTVEASGWRTQGVQ RLTHSLIKGIDQWVIEDVEECRLMLD+QGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQ VIKSARVMKKAVAHLLPFMEAEKLAKM+ADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYK           VYDMGVMQPCDLILAKAK+L VDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLSDRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRK IDFEKRPPVPPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLV IFPAATVDGEDVEVYANDSREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGVAD+MGMFAIACMGCEKMVKEFE+LNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYDRD
Sbjct:   15 MLSITQPHIIKEIHTKYVDSGSDIIATNTFSATTIAMADYEMEKDVYELNFESARLAREACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGGADIIIVETIFDTLNAKAGVFAVREYFDEQGITDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLAKVAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFAEEGLINMLGGCCGSTPAHIKA-------------MKDAVEPCSTRALPPPREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEKEKALKNKGKGTVEASGWRTQGVQERLTHSLIKGIDQWVIEDVEECRLMLDDQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQ-VIKSARVMKKAVAHLLPFMEAEKLAKMIADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYK-----------VYDMGVMQPCDLILAKAKQLKVDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLSDRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRKNIDFEKRPPVPPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLVAIFPAATVDGEDVEVYANDSREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADYMGMFAIACMGCEKMVKEFEDLNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYDRD 1224          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15628.1 vs. uniprot
Match: A0A7S2XWE9_9STRA (Methionine synthase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XWE9_9STRA)

HSP 1 Score: 1758 bits (4552), Expect = 0.000e+0
Identity = 900/1236 (72.82%), Postives = 1026/1236 (83.01%), Query Frame = 0

Query:    1 MLSITQPHIIKEIHTKYLESG-SDIIGTNTFSATTIAMADYEMEKDVYELNFESARLAKEACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGGSDIIIVETIFDTLNAKAAVFAVREYFDEQGITDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLAQVAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFAQEGLINMLGGCCGSTPPHIKARRKGRAEKKIKRAMKDAVEPCSTRALPPPREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEKEKALKNKGKGTVEASGWRTQGVQARLTHSLIKGIDQWVIEDVEECRLMLDEQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQQVIKSARVMKKAVAHLLPFMEAEKLAKMVADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASELASATRFAVYDMGVMQPCDLILAKAKELNVDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLSDRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRKGIDFEKRPPVPPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLVGIFPAATVDGEDVEVYANDSREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADHMGMFAIACMGCEKMVKEFEELNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYDRD 1235
            MLSITQP IIKEIH KYLE G S +IGTNTFS+TTIAMADY+ME  V ELN ES RLA+EAC+E TAKDPT+PRFV+GA+GPTNRTGSISP+VEDPS RNVTFDEL +AY +Q  GL++GG DI++VETIFDTLNAKAAVFA   + +E G+ D+PLFVSGTLVDQSGRTLSGQTTE FYVS+RHCKPFC+GLNCALGA+QM+PFL+RLA VAECFVHVYSNAGLPNAMGGYD+TP+DMAR+NL+FA E L+NM+GGCCGSTPPHIKA             + +AV+    R LP  +EP +WLSGLEDL+V+K+RF FLNVGERCN++GSIRFKKLIL GDY TAMD+AK+QVEDGAMV+DIN+DDG+LDG+AAMQKF KIAVTEP++SKVPFMIDSSKF IVEAG+KWVQGKSI+NSISLKVGEEEFKR A I+K HGAAV+VMAFDEEGQAA    KVRICKRSYD+LV+EV FPPEDIIFDPNILTIATGI+EHNNY VDFI+AT++IKEICPY +ISGGVSNLSFGFRGVNVIRE++HSVFL+HAV AGMDMGIVNAGM+EVYDDI   LL++VEDAV NRHS ATE LL+ +  EKEKA    G    E   WR + V  RLT++LIKGI Q++ EDVEECR  +D    RPL VIEGPLM GMN+VGDLFGAGKMFLPQ VIKSARVMKKAVA+LLPFME EK A + A GI    + ++DDS YAGKVL+ATVKGDVHDIGKNIV+VVLGCNNYK           V+D+GVMQPC+ IL   K+   DV+GLSGLITPSLDEMVFVAKEM K G K PL+IGGATTSKMHTAVKISPQYA+ E PVIHVLDASRSVTVV +LL D K ++VE++MEEYE+LREEHYAG EER +L  ++A  K   IDF   PPV  PN+ G+TV+  SY L+DVL YIDWNPFFQTWELRGRYPNRGYPKIFNDE+VG EAKKLF+EAQ MLK IV  K L+L G+ GIF A  V GEDVEVY    + EV A+FCMLRQQAEKES+ PYLS ADFIAPK+SG+ D+MGMFA+ACMG E+    FE  NDDYSKIMVQAL DR VEAFAE LH DMR  IWGYAPDEK+  +DL K++YQGIRPAPGYPSQPDHTEK TMW+LL   E +GI LSE+LSM+PASSVSAL FAHP +EYFAVG I KDQV  YAERKGM++   EKWLSPIL+YDR+
Sbjct:   77 MLSITQPQIIKEIHLKYLEEGNSQLIGTNTFSSTTIAMADYDMESYVRELNVESVRLAREACDEVTAKDPTRPRFVVGAIGPTNRTGSISPNVEDPSVRNVTFDELEIAYYEQVCGLVEGGCDILMVETIFDTLNAKAAVFACNRFLEEHGLEDMPLFVSGTLVDQSGRTLSGQTTEAFYVSIRHCKPFCVGLNCALGATQMKPFLRRLAVVAECFVHVYSNAGLPNAMGGYDDTPDDMARENLIFADEKLVNMIGGCCGSTPPHIKA-------------ISEAVKGMPPRPLPEKKEPVLWLSGLEDLVVSKDRFTFLNVGERCNIAGSIRFKKLILGGDYATAMDIAKKQVEDGAMVLDINVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGMKWVQGKSIINSISLKVGEEEFKRQAGIIKRHGAAVIVMAFDEEGQAATESEKVRICKRSYDILVNEVQFPPEDIIFDPNILTIATGIEEHNNYGVDFIEATKKIKEICPYARISGGVSNLSFGFRGVNVIREAIHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPKDLLQLVEDAVLNRHSEATEKLLERSLEEKEKAASGGGPKKKEVE-WRIKPVTERLTYALIKGISQYIDEDVEECRQTVD----RPLQVIEGPLMQGMNIVGDLFGAGKMFLPQ-VIKSARVMKKAVAYLLPFMEEEKKAALEAKGIT--EMPEDDDSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYK-----------VFDIGVMQPCESILDAIKKYQPDVVGLSGLITPSLDEMVFVAKEMEKAGLKQPLMIGGATTSKMHTAVKISPQYANNEHPVIHVLDASRSVTVVQSLLGDDKDEFVEDLMEEYEDLREEHYAGLEERCFLALDQAKQKGLQIDFAACPPVQAPNQLGVTVLD-SYTLEDVLPYIDWNPFFQTWELRGRYPNRGYPKIFNDENVGGEAKKLFDEAQEMLKDIVANKRLHLKGVCGIFAANRV-GEDVEVYTGADKSEVAAKFCMLRQQAEKESEAPYLSMADFIAPKDSGLDDYMGMFAVACMGAEEEAARFEAENDDYSKIMVQALADRLVEAFAEALHRDMRVSIWGYAPDEKLPAEDLHKIRYQGIRPAPGYPSQPDHTEKTTMWNLLQVEEKSGIRLSEALSMLPASSVSALCFAHPQAEYFAVGKIEKDQVVEYAERKGMEVAEMEKWLSPILNYDRE 1278          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15628.1 vs. uniprot
Match: A0A836CPG7_9STRA (Methionine synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CPG7_9STRA)

HSP 1 Score: 1723 bits (4463), Expect = 0.000e+0
Identity = 892/1237 (72.11%), Postives = 1009/1237 (81.57%), Query Frame = 0

Query:    1 MLSITQPHIIKEIHTKYLESGSDIIGTNTFSATTIAMADYEMEKDVYELNFESARLAKEACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGGSDIIIVETIFDTLNAKAAVFAVREYFDEQGITDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLAQVAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFAQEGLINMLGGCCGSTPPHIKARRKGRAEKKIKRAMKDAVEPCSTRALPPPREP-FMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEKEKALKNK--GKGTVEASGWRTQGVQARLTHSLIKGIDQWVIEDVEECRLMLDEQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQQVIKSARVMKKAVAHLLPFMEAEKLAKMVADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASELASATRFAVYDMGVMQPCDLILAKAKELNVDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLSDRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRKGIDFEKRPPVPPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLVGIFPAATVDGEDVEVYANDSREEVLARFCMLRQQAEKESD-DPYLSQADFIAPKESGVADHMGMFAIACMGCEKMVKEFEELNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYD 1233
            +LSITQP II++IH +YLESGSDIIGTNTFS+TTIA ADY ME  VYELN+ESA+LA+ AC+E TA+DP++PRFV G++GPTNRT SISP VEDPS+RNV FDELV AY  + K L++GGSDIIIVETIFDTLNAKAAVFAVR+Y  E    D+PL +SGTLVDQSGRTLSGQT EGFYVSMRH KPFCIGLNCALGA QM+PFL+RL+ VAECFVHVYSNAGLPNAMGGYD+TP DMARDN  FA EG++NM+GGCCGSTP HIKA             M + V+ C+ R LP P +P  MWLSGLE L V+K+R  FLNVGERCN SGSI+FKKLI+ G+Y +AMDVAK QVEDGAMV+DINLDDGLLDG+AAMQKFCKIAVTEPDVSKVPFMIDSSKF I+EAGLKWVQGK IVNSISLKVGE EF+R A IV+ HGAAVVVMAFDEEGQAAD ++KVRICKRSYD+LVDE+ FPPEDIIFDPNILTIATGIDEHNNYA+DFI AT RIKE+CP+CKISGGVSNLSFGFRGVN IRE+MHSVFL+HA++AGMDMGIVNAGML+VYDDI P LLKIVEDAV N+H GATEALL+ A+IE+E A   K  GK  V+   WR Q V+ARLTHSLIKGIDQ++  D EE R     Q  RPL VIEGPLM GMNVVGDLFGAGKMFLPQ VIKSARVMKKAVA+LLPFME EK  K +  G+DP+   D DD+ YAGKV+IATVKGDVHDIGKNIVAVVLGCNNYK           VYD+GVM  C+ IL    +   D++GLSGLITPSLDEMVFVA+EMAK G   PL+IGGATTSKMHTAVKI+PQY+SPE PVIHVLDASRSVTVV +LL   K +Y EE++EEYEELRE              EKA  KR  I+FE  P   PP+  G  V+  +Y L+ VLDYIDWNPFFQTWELRGRYPNRGYPKIFND +VG EAKKLF++AQVML  IV  K L L G++G++PA  V GEDV +Y++ SR E LA FCMLRQQAEK++  + YLS ADF+AP   G  DH+GMFA+ACMGC+  VK FE  NDDYSKIMVQAL DR VEAFAE LH D+R++IWG+A +EKV G+DLFKLKYQGIRPAPGYPSQPDHTEKRTMW++L A EL GI+LSESLSMMPASSVSALVFAHP SEYFAVG I KDQV SYA RKGM L+ C++WL PIL+YD
Sbjct:   79 LLSITQPQIIRQIHIEYLESGSDIIGTNTFSSTTIAQADYGMENLVYELNYESAKLARSACDEVTARDPSRPRFVAGSIGPTNRTASISPSVEDPSYRNVNFDELVDAYYFEAKALVEGGSDIIIVETIFDTLNAKAAVFAVRKYLRETNQEDIPLMISGTLVDQSGRTLSGQTGEGFYVSMRHVKPFCIGLNCALGAQQMKPFLRRLSMVAECFVHVYSNAGLPNAMGGYDDTPSDMARDNHEFASEGMVNMIGGCCGSTPQHIKA-------------MHEDVKDCAPRPLPKPLDPPVMWLSGLEPLQVSKDRINFLNVGERCNCSGSIQFKKLIMAGNYSSAMDVAKAQVEDGAMVVDINLDDGLLDGLAAMQKFCKIAVTEPDVSKVPFMIDSSKFEILEAGLKWVQGKCIVNSISLKVGETEFRRQATIVRDHGAAVVVMAFDEEGQAADEESKVRICKRSYDILVDELKFPPEDIIFDPNILTIATGIDEHNNYAIDFINATRRIKEVCPHCKISGGVSNLSFGFRGVNTIREAMHSVFLYHAIQAGMDMGIVNAGMLQVYDDIAPDLLKIVEDAVLNKHPGATEALLERAQIERELADARKAGGKVAVKEGSWRDQPVKARLTHSLIKGIDQYIETDTEEAR----SQADRPLSVIEGPLMDGMNVVGDLFGAGKMFLPQ-VIKSARVMKKAVAYLLPFMEEEKRLKALEQGVDPNEPVD-DDAAYAGKVVIATVKGDVHDIGKNIVAVVLGCNNYK-----------VYDLGVMVSCEDILKAVDKYKPDIVGLSGLITPSLDEMVFVAREMAKKGCTQPLMIGGATTSKMHTAVKIAPQYSSPEHPVIHVLDASRSVTVVSSLLGGEKTEYAEEVLEEYEELREXXXXXXXXXXXXTLEKAMGKRLEINFEATPAPQPPSFIGNKVLD-NYSLESVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDATVGAEAKKLFDDAQVMLNDIVANKRLRLTGVLGMYPANRV-GEDVVLYSDASRAEKLATFCMLRQQAEKDTPTEAYLSLADFVAPV--GHRDHLGMFAVACMGCDDEVKRFEAENDDYSKIMVQALADRLVEAFAEALHADVRREIWGFASEEKVAGEDLFKLKYQGIRPAPGYPSQPDHTEKRTMWNVLKAEELAGIQLSESLSMMPASSVSALVFAHPQSEYFAVGKIGKDQVESYAARKGMPLDECQRWLGPILNYD 1281          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15628.1 vs. uniprot
Match: A0A7S4DBJ3_HETAK (Methionine synthase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DBJ3_HETAK)

HSP 1 Score: 1719 bits (4453), Expect = 0.000e+0
Identity = 886/1240 (71.45%), Postives = 1010/1240 (81.45%), Query Frame = 0

Query:    1 MLSITQPHIIKEIHTKYLESG-SDIIGTNTFSATTIAMADYEMEKDVYELNFESARLAKEACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGGSDIIIVETIFDTLNAKAAVFAVREYFDEQGITDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLAQVAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFAQEGLINMLGGCCGSTPPHIKARRKGRAEKKIKRAMKDAVEPCSTRALPPPREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEKEKALKNKGKGTVEASGWRTQGVQARLTHSLIKGIDQWVIEDVEECRLMLDEQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQQVIKSARVMKKAVAHLLPFMEAEKLAKMVADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASELASATRFAVYDMGVMQPCDLILAKAKELNVDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLS-DRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRKGIDFEKRPPVPPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLVGIFPAA-TVDGEDVEVYANDSREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESG--VADHMGMFAIACMGCEKMVKEFEELNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYDRD 1235
            MLS+TQP IIKEIH +YL  G S +IGTNTFS TTIAMADYEME  V ++NF SAR+A+E  +E TA DPT+PRFV+GA+GPTNRTGSISP VED S RNVTFDEL  AY  QT  L++GGSD+IIVETIFDTLNAKAA+FAV  YF+E G T+ PLF+SGTLVDQSGRTLSGQT E FY S+RH KPFC+GLNCALGA+QM+PFL+RLA V ECFVHVYSNAGLPNAMGGYD+TPEDMAR+N +FA E L+NM+GGCCGSTPPHI A             +   VE    R LP   EP MWLSGLEDLIV+K+RF FLNVGERCN++GSI FKK I+ GDY  AM++AK+QVEDGAMVID+N+DDG+LDG+AAMQKF KIAVTEP++SKVPFMIDSSKF IVEAGLKWVQGKSIVNSISLKVGEEEFKRHA I K HG A+VVMAFDE GQAA  D+KV +CKRSYD+LVDEVGFPPEDIIFDPNILT+ATG++EHNNY VDFI AT RIKE CP+ KISGGVSNLSFGFRGVNVIRE+MHSVFL+HAV AGMDMGIVNAGM+EVYDDI   LL +VEDAV NR   ATE LLD + +E+EK     GK  V+A  WRT+ V  RLTH+LIKGID+++  DVEECR  +     +PL+VIEGPLM+GMNVVGDLFGAGKMFLPQ VIKSARVMKKAVA+LLPFMEAEK AK+ A G+DP   D+  DS YAGKVL+ATVKGDVHDIGKNIV+VVLGCNNYK           V+D+GVM  C+ ILAKA+E  VDVIGLSGLITPSLDEMVFVAKEMAK G K PL+IGGATTSKMHTAVKI+PQYA+ E PVIHVLDASRSVTVV +LLS  +K  YVEE++EEYEELREEHYA  EER YL F K  +K   +DF  RPPV  P  PG+  + G YEL DV+ +IDWNPFFQTWELRGRYPNRGYPKIFNDE+VG EA+KLF++AQ ML+ IV  K L L G+  IFPAA T DGEDVEV++ +     L +FCMLRQQAEKE+D+PYLS ADF+AP+  G  V DH+G FA+AC+G ++     E  NDDY+KIMVQAL DR VEAFAEKLH D+R  +WGYAPDE++  +DL K++YQGIRPAPGYPSQPDHTEKRTMWDLL   EL+GI LSESLSMMPASSVSALVFAHPD++YFAVG I +DQV  YA RKGMD+   EKWLSPIL+YDRD
Sbjct:   80 MLSLTQPEIIKEIHRQYLVEGQSQLIGTNTFSGTTIAMADYEMEAWVDDINFHSARIAREVADEVTAADPTRPRFVVGAIGPTNRTGSISPSVEDASQRNVTFDELEAAYYQQTVKLMEGGSDVIIVETIFDTLNAKAAIFAVNRYFEETGNTETPLFISGTLVDQSGRTLSGQTGEAFYASVRHAKPFCVGLNCALGAAQMKPFLRRLANVVECFVHVYSNAGLPNAMGGYDDTPEDMARENHIFASERLVNMIGGCCGSTPPHIAA-------------IAADVEGMPPRELPTLGEPTMWLSGLEDLIVSKDRFSFLNVGERCNLAGSILFKKKIMAGDYMAAMEIAKKQVEDGAMVIDVNVDDGMLDGVAAMQKFLKIAVTEPEISKVPFMIDSSKFEIVEAGLKWVQGKSIVNSISLKVGEEEFKRHATICKKHGCAIVVMAFDENGQAATEDDKVAMCKRSYDILVDEVGFPPEDIIFDPNILTVATGLEEHNNYGVDFINATRRIKEQCPHAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVAAGMDMGIVNAGMMEVYDDIPADLLALVEDAVLNRRPDATERLLDRSLVEREKQAAAGGK-VVKAVEWRTRPVAERLTHALIKGIDEFIDGDVEECRQAV----AKPLEVIEGPLMNGMNVVGDLFGAGKMFLPQ-VIKSARVMKKAVAYLLPFMEAEKAAKLAAQGLDPQEADE--DSMYAGKVLMATVKGDVHDIGKNIVSVVLGCNNYK-----------VFDIGVMCQCEDILAKAQEYKVDVIGLSGLITPSLDEMVFVAKEMAKLGLKQPLMIGGATTSKMHTAVKIAPQYATGEHPVIHVLDASRSVTVVQSLLSATQKEAYVEELLEEYEELREEHYASLEERVYLDFAKVRAKGLQVDFAARPPVAAPRAPGVQALEG-YELADVVPFIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGAEARKLFDDAQAMLRDIVDNKKLRLTGVNAIFPAARTEDGEDVEVFSPEGGHSSLGKFCMLRQQAEKETDEPYLSLADFVAPRGEGGAVRDHLGAFAVACLGADEEAARHEAANDDYNKIMVQALADRLVEAFAEKLHRDIRTALWGYAPDEELPAEDLHKIRYQGIRPAPGYPSQPDHTEKRTMWDLLKVEELSGIRLSESLSMMPASSVSALVFAHPDAQYFAVGKIGRDQVQEYAARKGMDVPEMEKWLSPILNYDRD 1286          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15628.1 vs. uniprot
Match: A0A097PA47_9STRA (Methionine synthase n=3 Tax=Monodopsidaceae TaxID=425072 RepID=A0A097PA47_9STRA)

HSP 1 Score: 1688 bits (4372), Expect = 0.000e+0
Identity = 864/1241 (69.62%), Postives = 1010/1241 (81.39%), Query Frame = 0

Query:    1 MLSITQPHIIKEIHTKYLESGSDIIGTNTFSATTIAMADYEMEKDVYELNFESARLAKEACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGGSDIIIVETIFDTLNAKAAVFAVREYFDEQGITD-VPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLAQVAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFAQEGLINMLGGCCGSTPPHIKARRKGRAEKKIKRAMKDAVEPCSTRALPPPREPFMWLSGLEDLIVTKERFQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAVKAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEKEKALKNKGKGTVEASGWRTQGVQARLTHSLIKGIDQWVIEDVEECRLMLDEQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQQVIKSARVMKKAVAHLLPFMEAEKLAKMVADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASELASATRFAVYDMGVMQPCDLILAKAKELNVDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLSDRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRKGIDFEKRPPV-PPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLVGIFPAATVDGEDVEVYAN----DSREEVLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADHMGMFAIACMGCEKMVKEFEELNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYDRD 1235
            +L++TQP IIK+IH KYL+ G+D+IGTNTFS+TTIA ADY+ME   YELN ESAR+A+E  +E +A++P +PRFV G++GPTNRT SISPDV D S RNVTFDEL  AY ++ +GLIDGG D II+ETIFDTLNAKAA+F+VR    E+G+ + +PL +SGTLVDQSGRTLSGQTTE FYVS+RHC PFC+GLNCALGA QMRPFLQRL++VAECFV VYSNAGLPNAMGGYD+TP DMA DN +F +E L+NM+GGCCGSTP HI A  K          M + V P         R   MWLSGLEDL+V+KERFQFLNVGERCN++GS  FKKLIL GDY  AMD+AK+QVEDGAMV+D+N+D+GLLDG+AAMQ+F KIA+TEP+ SKVP M+DSSKF I+EAGLKWVQGK IVNSISLKVGEEEFKR A +VKAHGAAVVVMAFDEEGQAAD  NKVRICKRSYD+LVDEVGFPPEDI+FDPNILTIATGI+EHNNYAVDFI+A   IKE+CP+ KISGGVSNLSFGFRGVNVIRE+MHSVFL+HAV+AGMDMGIVNAGML+VY DI P LL+IVEDAV N+H+ ATEALL+ + +E+EKAL NKG+ T   + WRT  VQARLTH+L+KGID+++ EDVEECR ++     RPL+VIEGPLMSGMNVVGDLFG+GKMFLPQ VIKSARVMKKAVA+LLPFME EK  KM   GI+  +   +DDS YAG VL+ATVKGDVHDIGKNIV VVLGCNNYK           VYDMGVM PC+ I+ KA E+   VIGLSGLITPSLDEMVFVAKEM+K G  +PLLIGGATTSKMHTAVKI+P Y++ E P IHVLDASRSVTVVG+LL + K +YV+++++EY+ELREEHYAG EERH   F+ A      IDF   PPV  P    G+ VV   Y L+DV+ +IDWNPFFQTWELRG+YPNRGYP+IFNDE+VGEEAKKLF +AQ +LK +V  K L+L G+VG+FPAA V GEDVEVYA+    D  EE + +FCMLRQQ EKESDD Y S ADF+A KESG  D++G FA+AC G E+ V+ +E LNDDY+KIMVQAL DRFVEAFAE LH +MR   WGYAP+E+++ +DL K+KY GIRPAPGYPSQPDHTEK  MW+LL A EL GI L+ESLSM PASSVSALVFAHP+S+YFAVG I KDQV  YA RKGM +E  E+WL+PIL+YDRD
Sbjct:   75 LLTLTQPTIIKDIHRKYLKGGADLIGTNTFSSTTIAQADYDMESLAYELNVESARIAREVADEVSAEEPERPRFVGGSIGPTNRTASISPDVVDASKRNVTFDELDEAYYEEARGLIDGGCDFIIIETIFDTLNAKAAIFSVRRLLKERGMEETMPLIISGTLVDQSGRTLSGQTTEAFYVSVRHCNPFCVGLNCALGAKQMRPFLQRLSKVAECFVSVYSNAGLPNAMGGYDDTPADMAADNSVFVKESLVNMIGGCCGSTPDHINAIAK----------MCEGVPPRPVHE----RVKHMWLSGLEDLVVSKERFQFLNVGERCNIAGSPLFKKLILAGDYAKAMDIAKKQVEDGAMVLDVNMDEGLLDGLAAMQRFLKIAMTEPECSKVPIMVDSSKFEIIEAGLKWVQGKCIVNSISLKVGEEEFKRQAAVVKAHGAAVVVMAFDEEGQAADEYNKVRICKRSYDILVDEVGFPPEDIVFDPNILTIATGIEEHNNYAVDFIKACRTIKEVCPHAKISGGVSNLSFGFRGVNVIREAMHSVFLYHAVQAGMDMGIVNAGMLQVYSDIPPDLLQIVEDAVLNKHAEATEALLERSMVEREKALANKGQATAVQAPWRTLEVQARLTHALVKGIDEFIDEDVEECRQLVP----RPLEVIEGPLMSGMNVVGDLFGSGKMFLPQ-VIKSARVMKKAVAYLLPFMEKEKEEKMKEQGIEAVA---DDDSAYAGVVLLATVKGDVHDIGKNIVGVVLGCNNYK-----------VYDMGVMIPCEKIIEKAIEVKAQVIGLSGLITPSLDEMVFVAKEMSKAGMTVPLLIGGATTSKMHTAVKIAPNYSTVEHPCIHVLDASRSVTVVGSLLGEDKEEYVQDVLDEYDELREEHYAGLEERHLKTFDAAKKLGFNIDFGASPPVCSPVGGTGVKVVD-YYSLEDVVPFIDWNPFFQTWELRGKYPNRGYPRIFNDENVGEEAKKLFADAQALLKDLVDNKRLSLRGVVGVFPAARV-GEDVEVYASEEAKDRGEEAIGKFCMLRQQQEKESDDAYFSLADFVASKESGHKDYLGAFAVACFGAEEEVERYEALNDDYNKIMVQALADRFVEAFAEALHREMRMKTWGYAPEEELDPEDLLKIKYDGIRPAPGYPSQPDHTEKVMMWELLKARELAGIGLTESLSMTPASSVSALVFAHPESQYFAVGRIEKDQVEDYAVRKGMVVEEVERWLAPILNYDRD 1280          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15628.1 vs. uniprot
Match: B7GBG7_PHATC (Methionine synthase n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B7GBG7_PHATC)

HSP 1 Score: 1628 bits (4216), Expect = 0.000e+0
Identity = 836/1248 (66.99%), Postives = 994/1248 (79.65%), Query Frame = 0

Query:    1 MLSITQPHIIKEIHTKYLESG-SDIIGTNTFSATTIAMADYEMEKDVYELNFESARLAKEACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGGSDIIIVETIFDTLNAKAAVFAVREYFDEQGITDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLAQVAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFAQEGLINMLGGCCGSTPPHIKARRKGRAEKKIKRAMKDAVEPCSTRALPPPREPFMWLSGLEDLIVTKERFQ----FLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAV-KAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEKEKALKNKGKGTVEA---SGWRTQGVQARLTHSLIKGIDQWVIEDVEECRLMLDEQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQQVIKSARVMKKAVAHLLPFMEAEKLAKMVADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASELASATRFAVYDMGVMQPCDLILAKAKELNVDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLSDRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRKGIDFEKRPPVPPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLVGIFPA-ATVDGEDVEVYANDSREE---VLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADHMGMFAIACMGCEKMVKEFEELNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYDRD 1235
            MLSI+QPHIIK I+ +YLE G S++IGTNTFS+TTIAMADY+ME   YELN+  ARLA+EAC+E TAKDP+KPRFV+GA+GPTNRT SISP VEDP+ RNVTFDELV  Y +Q  GL+DGG+D+++VETIFDTLNAKAA++A+ E+ +  G+ D+P+FVSGTLVDQSGRTLSGQT E FYVS+RH KP C+GLNCALGA  M PF++RL++ AECFVHVYSNAGLPNAMGGYD+TPEDMAR+N +F + G +NM+GGCCGSTPPHIKA R             +  E    R LP    P MWLSGLEDL V     Q    FLNVGERCN++GSI+FKKL++KGDY  AMD+AKQQVEDGA VIDIN+DDG+LDG+AAMQKF KIAVTEP+++KVPFM+D+SKF IV AGLKW QGK IVNSISLKVGEE F   A ++K HGAAVVVMAFDE+GQAA  D K+RICKRSYD+LV++VGFPPEDI+FDPN+LTI TG++EH NY VDFI + +RIKE CPY KISGG+SNLSFGFRGV  IRES+H+VFL HA+ ++GMD+GIVN+  L   DD+EP +  + E+ V N+   AT+ +L+    E+ K       G V+    S WR      RL HSL+ GI ++V  DVEE R++      +PLDVIEGPLM GMNVVGDLFGAGKMFLPQ VIKSARVMKKAVA+LLPFM+AEK   M+A G+DPD +D +DDS YAGKVL+ATVKGDVHDIGKNIVAVVLGCNNYK           VYD+GVM  C+ ILAKAKE NVDVIGLSGLITPSLDEMV VAKEM+K GFK PLLIGGATTSKMHT+VKI+P Y +PE P IHVLDASRSVTVV +LL + K ++VE+IMEEYEE+RE++YAG E+R++L FEKA  ++  I+F++ P    PNK GITV+  + ++  V+ YIDWNPFFQTWELRGRYPNRGYPKIFNDE+VG EAKKLF +AQ ML +I ++ S+ L G+VGIFPA  + DGEDV VY  ++  E   V   FCMLRQQAEKESDDPYLSQADF+AP  +G  DH+GMFA++C GC+ +VK+FE  NDDYSKIM QAL DRFVEAFAE +H +MR D+WGYA  E++   DL K+KY GIRPAPGYPSQPDHTEKRTMW+LL A EL GIELSESLSMMPASSVSALVFAHP+SEYFAVG + KDQV++YA RK MDL  CE+WLSPIL+Y+RD
Sbjct:   37 MLSISQPHIIKGIYMQYLEEGGSNMIGTNTFSSTTIAMADYQMEAYAYELNYVGARLAREACDEVTAKDPSKPRFVVGAIGPTNRTCSISPSVEDPAARNVTFDELVETYLEQVVGLVDGGADVLMVETIFDTLNAKAALYAIGEFLEFSGL-DIPVFVSGTLVDQSGRTLSGQTGEAFYVSIRHAKPMCVGLNCALGAKHMVPFVERLSKAAECFVHVYSNAGLPNAMGGYDDTPEDMARENEVFFENGWLNMVGGCCGSTPPHIKAIR-------------ETAERYQPRKLPDVGRPKMWLSGLEDLKVEDVHNQLGLPFLNVGERCNIAGSIKFKKLMMKGDYGAAMDIAKQQVEDGAHVIDINVDDGMLDGLAAMQKFVKIAVTEPEIAKVPFMLDASKFDIVMAGLKWCQGKCIVNSISLKVGEELFMEQATLLKKHGAAVVVMAFDEQGQAATEDEKIRICKRSYDVLVNKVGFPPEDIVFDPNVLTIGTGMEEHANYGVDFINSVKRIKEECPYVKISGGISNLSFGFRGVTKIRESIHAVFLHHAITESGMDVGIVNSHELLSVDDLEPDMKLLCENLVFNKTPDATDDMLERTNFERSKG------GVVDEVKKSSWRDLEPSKRLEHSLVNGISEFVDSDVEEARVL----ATKPLDVIEGPLMDGMNVVGDLFGAGKMFLPQ-VIKSARVMKKAVAYLLPFMDAEKRENMIAAGLDPDDVDPDDDSNYAGKVLMATVKGDVHDIGKNIVAVVLGCNNYK-----------VYDIGVMCSCEKILAKAKEYNVDVIGLSGLITPSLDEMVDVAKEMSKQGFKQPLLIGGATTSKMHTSVKIAPSYTTPEHPTIHVLDASRSVTVVNSLLGENKGEFVEDIMEEYEEMREDYYAGLEDRNFLTFEKAKEQKITINFDQSPVADTPNKIGITVID-TVKISAVVPYIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGGEAKKLFHDAQTMLNQICEDGSMTLKGVVGIFPANRSEDGEDVHVYETEADRESGNVSTTFCMLRQQAEKESDDPYLSQADFVAP--AGYKDHLGMFAVSCFGCDALVKKFESENDDYSKIMAQALADRFVEAFAEYVHREMRLDLWGYAAGEQLNESDLLKVKYDGIRPAPGYPSQPDHTEKRTMWNLLQAQELAGIELSESLSMMPASSVSALVFAHPESEYFAVGQVGKDQVTNYASRKQMDLALCERWLSPILNYERD 1245          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15628.1 vs. uniprot
Match: A0A1Z5K9H8_FISSO (Methionine synthase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K9H8_FISSO)

HSP 1 Score: 1624 bits (4206), Expect = 0.000e+0
Identity = 835/1249 (66.85%), Postives = 993/1249 (79.50%), Query Frame = 0

Query:    1 MLSITQPHIIKEIHTKYLESG-SDIIGTNTFSATTIAMADYEMEKDVYELNFESARLAKEACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGGSDIIIVETIFDTLNAKAAVFAVREYFDEQGITDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLAQVAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFAQEGLINMLGGCCGSTPPHIKARRKGRAEKKIKRAMKDAVEPCSTRALPPPREPFMWLSGLEDLIVTKER----FQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAV-KAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEKEKALKNKGKGTV----EASGWRTQGVQARLTHSLIKGIDQWVIEDVEECRLMLDEQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQQVIKSARVMKKAVAHLLPFMEAEKLAKMVADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASELASATRFAVYDMGVMQPCDLILAKAKELNVDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLSDRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRKGIDFEKRPPVPPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLVGIFPA-ATVDGEDVEVYANDSREEV---LARFCMLRQQAEKESDDPYLSQADFIAPKESGVADHMGMFAIACMGCEKMVKEFEELNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYDRD 1235
            MLSITQPHII+ I+ +YLE G SD+IGTNTFS+TTIAMADYEME   YELN+  ARLA+E C+E TA+DP KPRFV+GA+GPTNRTGSISP VEDPS RNVTFDELV  Y +Q  GL+DGG+DI++VETIFDTLNAKAA++A+ EY +  G+ D+P+FVSGTLVDQSGRTLSGQT E FY S+RH KP C+GLNCALGA  M PF++RLA   ECF+HVYSNAGLPNAMGGYD++PE MA  N LF + G +NM+GGCCGSTPPHI             RA+++  E    R LP    P MWLSGLED+ V          FLNVGERCN++GSI+FKKL++ GDY  AMD+AKQQVEDGA VIDIN+DDG+LDG+ AMQKF KIAVTEP+V+KVPFM+D+SKF IV AGLKW QGK IVNSISLKVGEE F  HA ++K HGAAVVVMAFDEEGQAA    KVRICKRSYD+LV++V FPPEDIIFDPN+LTI TG++EH NY VDFI A + IKE CPY KISGG+SNLSFGFRGV  IRES+H+VFL HA+ ++GMD+GIVNA  + + +++EP LLK  E+ V N+   ATE +L+    EKE+    K  G V    EAS WR Q    RL H+LI GI ++V +DVEE R    ++  +PLDVIEGPLM GMN+VGDLFGAGKMFLPQ VIKSARVMKKAVA+LLPFM+ EK   ++A G++PD  D  DDS YAGKVL+ATVKGDVHDIGKNIVAVVLGCNN+K           VYD+GVM  C+LIL KAKE +VDVIGLSGLITPSLDEMV VAKE++KGGFK PLLIGGATTSKMHTAVKI+P Y+S E PVIHVLDASRSVTVV +LL + K DYV +I EEYEE+RE++YAG E+R++L FE+A  ++  IDFEK PP   P + G T++    +L DV+ YIDWNPFFQTWELRGRYPNRGYPKIFNDE+VG+EAKKLF++AQ M+K+I+ + S+ L G+VG++PA  + DGEDVEVY N++  E     ARFCMLRQQAEKESDDPY SQADF+AP+  G+ DH+GMFA++C+GC+ +V ++E+ NDDYSKIM QAL DRFVEAFAE LH ++R ++WGYAPDEK+   DL K+KY GIRPAPGYPSQPDHTEK TMW+L+ A EL GIELSESLSMMPASSVSALVFAHP +EYFAVG + KDQV SYAERK M L+ CE+WLSPIL+Y+R+
Sbjct:  147 MLSITQPHIIQGIYRQYLEEGGSDLIGTNTFSSTTIAMADYEMEAYAYELNYWGARLAREVCDEVTARDPAKPRFVVGAIGPTNRTGSISPSVEDPSARNVTFDELVETYFEQVVGLMDGGADILMVETIFDTLNAKAALYAIGEYLEFSGL-DIPVFVSGTLVDQSGRTLSGQTGEAFYASIRHAKPMCVGLNCALGAQHMTPFVERLAGCVECFMHVYSNAGLPNAMGGYDDSPETMAEQNRLFFENGWLNMVGGCCGSTPPHI-------------RAIREVSEGYEPRKLPDVGRPKMWLSGLEDMKVEDVHNHLGLPFLNVGERCNIAGSIKFKKLMMAGDYGAAMDIAKQQVEDGAHVIDINVDDGMLDGLTAMQKFVKIAVTEPEVAKVPFMLDASKFEIVLAGLKWCQGKPIVNSISLKVGEEMFIEHATLLKKHGAAVVVMAFDEEGQAATESEKVRICKRSYDILVNKVKFPPEDIIFDPNVLTIGTGMEEHANYGVDFINACKTIKEECPYVKISGGISNLSFGFRGVTKIRESIHAVFLHHAILESGMDVGIVNAKEMLMIEEVEPELLKASENLVFNKSEDATEVMLELTRKEKERFEAFKAGGGVTKTNEAS-WRDQDAVKRLEHALINGISEFVDQDVEEAR----QKCSKPLDVIEGPLMDGMNIVGDLFGAGKMFLPQ-VIKSARVMKKAVAYLLPFMDEEKRQNLIAQGLNPDDFDQNDDSNYAGKVLMATVKGDVHDIGKNIVAVVLGCNNFK-----------VYDIGVMCSCELILEKAKEYDVDVIGLSGLITPSLDEMVVVAKELSKGGFKQPLLIGGATTSKMHTAVKIAPNYSSAEHPVIHVLDASRSVTVVSSLLGNAKEDYVADIHEEYEEMREDYYAGLEDRYFLTFEQAKQQKFMIDFEKSPPASQPKQIGTTLID-KVKLSDVVPYIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGKEAKKLFDDAQSMMKEIIADGSMWLTGVVGLYPANRSDDGEDVEVYENEADRETGTPKARFCMLRQQAEKESDDPYFSQADFVAPR--GMKDHLGMFAVSCLGCDALVDKYEKSNDDYSKIMAQALADRFVEAFAEYLHREIRVNMWGYAPDEKLNEADLLKIKYDGIRPAPGYPSQPDHTEKTTMWELIKAKELAGIELSESLSMMPASSVSALVFAHPQAEYFAVGQVGKDQVVSYAERKNMKLDVCERWLSPILNYERE 1361          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15628.1 vs. uniprot
Match: A0A7S4J684_9STRA (Methionine synthase n=2 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4J684_9STRA)

HSP 1 Score: 1617 bits (4187), Expect = 0.000e+0
Identity = 828/1248 (66.35%), Postives = 990/1248 (79.33%), Query Frame = 0

Query:    1 MLSITQPHIIKEIHTKYLE-SGSDIIGTNTFSATTIAMADYEMEKDVYELNFESARLAKEACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGGSDIIIVETIFDTLNAKAAVFAVREYFDEQGITDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLAQVAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFAQEGLINMLGGCCGSTPPHIKARRKGRAEKKIKRAMKDAVEPCSTRALPPPREPFMWLSGLEDLIVTKER----FQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAV-KAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEKEKA-LKNKGKGTVEA--SGWRTQGVQARLTHSLIKGIDQWVIEDVEECRLMLDEQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQQVIKSARVMKKAVAHLLPFMEAEKLAKMVADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASELASATRFAVYDMGVMQPCDLILAKAKELNVDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLSDRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRKGIDFEKRPPVPPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLVGIFPA-ATVDGEDVEVYANDSREEV---LARFCMLRQQAEKESDDPYLSQADFIAPKESGVADHMGMFAIACMGCEKMVKEFEELNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYDRD 1235
            MLS+T+P +I  I+ +YLE  GSD+IGTNTFS+TTIAMADYEME   YELN+E ARLA+ AC+E TA DP++PRFV+GA+GPTNRTGSISP VEDPS RNVTFDELV  Y +Q  GL+DGGSDI++VETIFDTLNAKAA++AV EY +  G+ D+P+FVSGTLVDQSGRTLSGQT E FY S+RH KP C+GLNCALGA  M PF+++LA+  ECF+HVYSNAGLPNAMGGYDETPE MA  N +F ++G +NM+GGCCGSTPPHIKA R             +  +  S R LP    P MWLSGLEDL+V          FLNVGERCN++GSI+FKKL++KGDY +AMD+AK QVEDGA VIDIN+DDG+LDG+AAMQKF KIAVTEP+V KVPFM+D+SKF IV AGLKW QGK IVNSISLKVGE+ FK  A ++K HGAAVVVMAFDE GQAA+ D KVRICKRSYDMLVDEVGFPPEDIIFDPN+LTI TG++EHN+Y +DFI AT+RIKE CPY KISGG+SNLSFGFRGV  IRES+H+VFL HA+ ++GMD+GIVNA  +    ++EP L    E+ V N+ + +T+ +LD  + EKE    + +G G VE   + WR      RL HSL+ GI ++V++DVEE R   D    +PLDVIEGPLM GMNVVGDLFGAGKMFLPQ VIKSARVMKKAVA+LLPFM+ EK   M+A G++PD  D+ D+S +AGKVL+ATVKGDVHDIGKNIV+VVLGCNNYK           VYD+GVM  C+ IL KAKE +VDVIGLSGLITPSLDEMV VAKEM+K GFK PLLIGGATTSKMHTAVK+SP Y + E PVIHVLDASRSVTVV +LL   K D+V +IMEEY+E+RE++YAG E+R++L F++A  ++  IDFE +PP P P K GITV+  S  L+D++ YIDWNPFFQTWELRGRYPNRGYPKIFNDE+VG+EAKKLF++A  M+   + EKS++L G+VG+FPA  + DGEDV VY +++        A FCMLRQQAEKE+DDPYLSQADF+AP  +G  DH+GMFA++C GC+ +V +FE  NDDYSKIM QAL DRFVEAFAE LH  +R ++WGYAPDE+++  DL K+KY GIRPAPGYPSQPDHTEKRTMWD++ A EL GI+LSESLSMMPA+SVSALVFAHPDS+YFAVG I KDQV SY  RK  +L   E+WLSPIL+Y+RD
Sbjct:  204 MLSMTRPDVISGIYKQYLEIGGSDMIGTNTFSSTTIAMADYEMEDYAYELNYEGARLARLACDEVTAADPSRPRFVVGAIGPTNRTGSISPSVEDPSVRNVTFDELVETYFEQAVGLVDGGSDILMVETIFDTLNAKAALYAVGEYLEFSGL-DIPVFVSGTLVDQSGRTLSGQTGEAFYASIRHAKPMCVGLNCALGAQHMVPFVEKLAKCVECFMHVYSNAGLPNAMGGYDETPEMMAEYNKVFFEKGWLNMVGGCCGSTPPHIKAIR-------------ECAQNFSPRPLPAVGRPKMWLSGLEDLVVDDVHNHLGLPFLNVGERCNIAGSIKFKKLMMKGDYGSAMDIAKAQVEDGAHVIDINVDDGMLDGLAAMQKFVKIAVTEPEVCKVPFMLDASKFEIVMAGLKWCQGKPIVNSISLKVGEKLFKEQATLLKKHGAAVVVMAFDENGQAAEEDEKVRICKRSYDMLVDEVGFPPEDIIFDPNVLTIGTGMEEHNDYGIDFINATKRIKEQCPYVKISGGISNLSFGFRGVTKIRESIHAVFLHHAILESGMDVGIVNAKEMLALSEVEPVLKTACENLVFNKTADSTDVMLDLTKKEKEAIEARKRGGGAVEVKKTSWRDLEATKRLEHSLVNGISEFVVKDVEEARQKCD----KPLDVIEGPLMDGMNVVGDLFGAGKMFLPQ-VIKSARVMKKAVAYLLPFMDKEKKEAMIAKGLNPDDFDENDESNFAGKVLMATVKGDVHDIGKNIVSVVLGCNNYK-----------VYDIGVMVSCEKILEKAKEYDVDVIGLSGLITPSLDEMVVVAKEMSKAGFKQPLLIGGATTSKMHTAVKLSPNYFTLEHPVIHVLDASRSVTVVSSLLGRDKGDFVTDIMEEYDEMREDYYAGLEDRYFLTFDQAKEQKCVIDFESKPPAPAPKKLGITVID-SVSLEDIVPYIDWNPFFQTWELRGRYPNRGYPKIFNDEAVGKEAKKLFDDAIAMMNTFISEKSMHLKGVVGLFPANRSEDGEDVHVYLDEAARAAGTPEATFCMLRQQAEKENDDPYLSQADFVAP--AGYKDHLGMFAVSCFGCDDLVNKFEAENDDYSKIMSQALADRFVEAFAEYLHRGIRTEMWGYAPDEELDEADLLKIKYDGIRPAPGYPSQPDHTEKRTMWDVIKAEELAGIQLSESLSMMPAASVSALVFAHPDSQYFAVGQIGKDQVESYGARKKFELSVMERWLSPILNYERD 1418          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15628.1 vs. uniprot
Match: A0A7S1ZPU5_9STRA (Methionine synthase n=3 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S1ZPU5_9STRA)

HSP 1 Score: 1617 bits (4187), Expect = 0.000e+0
Identity = 836/1249 (66.93%), Postives = 997/1249 (79.82%), Query Frame = 0

Query:    1 MLSITQPHIIKEIHTKYLE-SGSDIIGTNTFSATTIAMADYEMEKDVYELNFESARLAKEACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGGSDIIIVETIFDTLNAKAAVFAVREYFDEQGITDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLAQVAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFAQEGLINMLGGCCGSTPPHIKARRKGRAEKKIKRAMKDAVEPCSTRALPPPREPFMWLSGLEDLIVTKER----FQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAV-KAGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEKEKALKNKGKG----TVEASGWRTQGVQARLTHSLIKGIDQWVIEDVEECRLMLDEQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQQVIKSARVMKKAVAHLLPFMEAEKLAKMVADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASELASATRFAVYDMGVMQPCDLILAKAKELNVDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLSDRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRKGIDFEKRPPVPPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLVGIFPA-ATVDGEDVEVYAN-DSRE--EVLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADHMGMFAIACMGCEKMVKEFEELNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYDRD 1235
            MLSITQPH+I  I+ +YLE  GS++IGTNTFS+TTIAMADY ME   YELN+E ARLA++AC+E TA DPT PRFV+GA+GPTNRTGSISP VEDPS RNVTFDELV AY +Q  GL+DGG+DI++VETIFDTLNAKAA++AV EY +  G+ D+P+FVSGTLVDQSGRTLSGQT E FY S+RH KP C+GLNCALGA  M PF+++LA+V ECF+HVYSNAGLPNAMGGYDE+P+DMAR+N  F + G +NM+GGCCGSTPPHIKA R             +  E  + R LP    P MWLSGLEDL+V          FLNVGERCN+SGSIRFKKL++ GDY TAMD+AK+QVEDGA V+DIN+DDG+LDG+AAMQKF KIAVTEP+VSKVPFM+D+SKF IV AGLKW QGK IVNSISLKVGEE FK  A ++K HGAAVVVMAFDEEGQAA  D KVRICKRSYD+LV+EV FPPEDIIFDPN+LTI TG++EH NY VDFI A + IKE CPY KISGG+SNLSFGFRGV  IRES+H+VFL HA+ ++GMD+GIVNA  +    ++E  L K  E+ V N+   AT+A+L+  + EK +A++ + KG     ++   WR    + RL HSL+ GI Q++ +DVEE RLM      +PLDVIEGPLM GMNVVGDLFGAGKMFLPQ VIKSARVMKKAVA+LLPFME EK   ++  G+DP+  D EDDS YAGKVL+ATVKGDVHDIGKNIVAVVLGCNNYK           VYD+GVM  C+ IL KAKE NVDVIGLSGLITPSLDEMV VAKEM+K GFK PLLIGGATTSKMHTAVK+SP Y + E PVIHVLDASRSVTVV +LL + K D+V +IMEEY+E+RE++YAG E+R++L FE+A  ++  IDF+ + P P PNK GITVV    EL++V+ YIDWNPFFQTWELRGRYPNRGYPKIF+DE+VG EA+KLF++AQ MLK+I+ EKS++L G+VG+FPA  + DGEDV +Y++ DSR      A FCMLRQQAEKE++DPYLSQADFIAP  +G  DH+GMFA++C GCE +V+++E  +DDYSKIM QAL DRFVEAFAE LH+ +R ++WGYA +E++   DL K+KY GIRPAPGYPSQPDHTEK TMW+L+ A EL GI LS+SLSMMPA+SVSALVFAHPDS+YFAVG + KDQV SYAERK  +L   E+WLSPIL+Y+RD
Sbjct:  148 MLSITQPHVISGIYKEYLEVGGSNLIGTNTFSSTTIAMADYNMEAYAYELNYEGARLARQACDEVTAADPTNPRFVVGAIGPTNRTGSISPSVEDPSARNVTFDELVEAYFEQIVGLMDGGADILMVETIFDTLNAKAALYAVGEYLEFSGL-DIPVFVSGTLVDQSGRTLSGQTGEAFYASIRHAKPMCVGLNCALGAVHMIPFVEKLAKVVECFLHVYSNAGLPNAMGGYDESPDDMARNNKTFFENGWLNMVGGCCGSTPPHIKAIR-------------ECAENYAPRKLPDVGRPKMWLSGLEDLVVDDVHNHLGLPFLNVGERCNISGSIRFKKLMMAGDYATAMDIAKKQVEDGAHVLDINVDDGMLDGLAAMQKFVKIAVTEPEVSKVPFMLDASKFEIVLAGLKWCQGKCIVNSISLKVGEELFKEQATLLKKHGAAVVVMAFDEEGQAATEDEKVRICKRSYDILVNEVHFPPEDIIFDPNVLTIGTGMEEHANYGVDFINAVKTIKEQCPYVKISGGISNLSFGFRGVMKIRESIHAVFLHHAILESGMDVGIVNANEMLHVTEVEGELKKACENLVFNKTPDATDAMLELTQKEK-RAIEARKKGGAVTEIKEKSWRDFEAKKRLEHSLVNGISQYIDKDVEEARLM----SSKPLDVIEGPLMDGMNVVGDLFGAGKMFLPQ-VIKSARVMKKAVAYLLPFMEEEKKQALIDAGLDPEEFDPEDDSAYAGKVLMATVKGDVHDIGKNIVAVVLGCNNYK-----------VYDIGVMVSCETILEKAKEYNVDVIGLSGLITPSLDEMVVVAKEMSKAGFKQPLLIGGATTSKMHTAVKLSPNYFTLEHPVIHVLDASRSVTVVSSLLGENKEDFVTDIMEEYDEMREDYYAGLEDRYFLTFEQAKEQKFKIDFDAKSPAPAPNKVGITVVD-YVELENVVPYIDWNPFFQTWELRGRYPNRGYPKIFDDEAVGNEARKLFDDAQAMLKEIIAEKSMHLKGVVGLFPANQSEDGEDVHIYSDEDSRAAGNPTATFCMLRQQAEKENEDPYLSQADFIAP--AGYKDHLGMFAVSCFGCEALVEKYEAEHDDYSKIMSQALADRFVEAFAEYLHKVIRTEMWGYANNEELNEADLLKIKYDGIRPAPGYPSQPDHTEKTTMWNLIKAKELAGISLSDSLSMMPAASVSALVFAHPDSKYFAVGQVGKDQVESYAERKKFELPVAERWLSPILNYERD 1362          

BLAST of mRNA_E-siliculosus-1a_M_contig75.15628.1 vs. uniprot
Match: A0A088GJ40_9STRA (Methionine synthase n=1 Tax=Fragilariopsis kerguelensis TaxID=186038 RepID=A0A088GJ40_9STRA)

HSP 1 Score: 1610 bits (4170), Expect = 0.000e+0
Identity = 829/1248 (66.43%), Postives = 992/1248 (79.49%), Query Frame = 0

Query:    1 MLSITQPHIIKEIHTKYLE-SGSDIIGTNTFSATTIAMADYEMEKDVYELNFESARLAKEACEEATAKDPTKPRFVMGALGPTNRTGSISPDVEDPSFRNVTFDELVVAYKDQTKGLIDGGSDIIIVETIFDTLNAKAAVFAVREYFDEQGITDVPLFVSGTLVDQSGRTLSGQTTEGFYVSMRHCKPFCIGLNCALGASQMRPFLQRLAQVAECFVHVYSNAGLPNAMGGYDETPEDMARDNLLFAQEGLINMLGGCCGSTPPHIKARRKGRAEKKIKRAMKDAVEPCSTRALPPPREPFMWLSGLEDLIVTKER----FQFLNVGERCNVSGSIRFKKLILKGDYQTAMDVAKQQVEDGAMVIDINLDDGLLDGMAAMQKFCKIAVTEPDVSKVPFMIDSSKFPIVEAGLKWVQGKSIVNSISLKVGEEEFKRHARIVKAHGAAVVVMAFDEEGQAADCDNKVRICKRSYDMLVDEVGFPPEDIIFDPNILTIATGIDEHNNYAVDFIQATERIKEICPYCKISGGVSNLSFGFRGVNVIRESMHSVFLFHAVK-AGMDMGIVNAGMLEVYDDIEPGLLKIVEDAVNNRHSGATEALLDAAEIEK---EKALKNKGKGTVEASGWRTQGVQARLTHSLIKGIDQWVIEDVEECRLMLDEQGKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQQVIKSARVMKKAVAHLLPFMEAEKLAKMVADGIDPDSIDDEDDSQYAGKVLIATVKGDVHDIGKNIVAVVLGCNNYKASELASATRFAVYDMGVMQPCDLILAKAKELNVDVIGLSGLITPSLDEMVFVAKEMAKGGFKLPLLIGGATTSKMHTAVKISPQYASPETPVIHVLDASRSVTVVGALLSDRKPDYVEEIMEEYEELREEHYAGQEERHYLPFEKASSKRKGIDFEKRPPVPPPNKPGITVVTGSYELDDVLDYIDWNPFFQTWELRGRYPNRGYPKIFNDESVGEEAKKLFEEAQVMLKKIVKEKSLNLVGLVGIFPA-ATVDGEDVEVYANDSREE---VLARFCMLRQQAEKESDDPYLSQADFIAPKESGVADHMGMFAIACMGCEKMVKEFEELNDDYSKIMVQALTDRFVEAFAEKLHEDMRKDIWGYAPDEKVEGQDLFKLKYQGIRPAPGYPSQPDHTEKRTMWDLLNAAELTGIELSESLSMMPASSVSALVFAHPDSEYFAVGTIAKDQVSSYAERKGMDLETCEKWLSPILSYDRD 1235
            MLSI+QP II+ I+  YLE  GSD+IGTNTFS+TTIAMADY+ME  VYELN+ESARLA+E C+E TAKDPT+PRFV+GA+GPTNRTGSISP VEDP+ RNVTFDELV AY +Q  GL+DGG DI++VETIFDTLNAKAAV+AV EY +  G+ D+PLFVSGTLVDQSGRTLSGQT E FY S+RH KP  +GLNCALGAS M PF++RL++ AECFVHVY+NAGLPNAMGGYDE P DMA  N +F + G +NM+GGCCGSTP  IKA R             +  E   TR LP    P MWLSGLEDL+V          FLN+GERCN+SGSIRFKKL++ G+Y TAMD+AK+QVEDGA V+DIN+DDG+LDG+AAMQKF KIAVTEP+++KVPFM+D+SKF IV AGLKW QGK IVNSISLKVGE+ FK HA +++ HGAAVVVMAFDEEGQAA    KVRICKRSYDMLV+EV FPPEDI+FDPN+LTI TG++EH  Y +DFI A + IKEICPY KISGG+SNLSFGFRGV  IRES+H+VFL +A++ +GMD+GIVNA  + +Y +++  + +  ED + NR   ATE +L+  + EK   E   K  G   V    WRT+  + RL H+LI GI ++VIEDVE  RL    + ++PL VIEGPLM GMN+VGDLFGAGKMFLPQ VIKSARVMKKAVA+LLPFME EK  K +A+G+DPD ID++DDS YAGKV++ATVKGDVHDIGKNIVAVVLGCNNYK           VYD+GVM  C+LIL KAKE NVDVIGLSGLITPSLDEMV VAK+++K GFK PLLIGGATTSKMHTAVKI+P + + E PVIHVLDASRSVTVV +LL+  K ++VE++ EEY+E+RE++YAG E+R++L FE A  +RK IDF+  PP P PNK GITV+  S  L DV+ Y+DWNPFFQTWELRGRYPNRGYPKIF+D++VG EAKKLF++AQ M+++I++  S  L G+VG+FPA  + DGEDV+++ N++  E   + A+FCMLRQQAEKESDDP+LSQADFIAPK  G ADH+G FA++C GCE +  ++E   DD+SKIM Q+L DRFVEAFAE LH ++R DIWGYA  E ++  DL K+KY GIRPAPGYPSQPDHTEK TMWD++   E  GIELSESLSMMPA+SVSALVFAHP SEYFAVG I KDQV SYAERK MD+E CE+WLSPIL+YDRD
Sbjct:  146 MLSISQPDIIQGIYRGYLEVGGSDMIGTNTFSSTTIAMADYKMEDYVYELNYESARLAREVCDEVTAKDPTRPRFVVGAIGPTNRTGSISPSVEDPAARNVTFDELVDAYFEQIVGLVDGGCDILMVETIFDTLNAKAAVYAVGEYLEMSGL-DIPLFVSGTLVDQSGRTLSGQTGEAFYASIRHSKPMVVGLNCALGASHMIPFVERLSKCAECFVHVYANAGLPNAMGGYDEKPADMAAGNEVFFKNGWLNMVGGCCGSTPAXIKAIR-------------EVAEKYETRKLPDVGRPKMWLSGLEDLVVEDCHNHLGLPFLNIGERCNISGSIRFKKLMMAGNYGTAMDIAKKQVEDGAHVLDINVDDGMLDGIAAMQKFVKIAVTEPEIAKVPFMLDASKFEIVMAGLKWCQGKPIVNSISLKVGEKLFKEHATLLRKHGAAVVVMAFDEEGQAATESEKVRICKRSYDMLVNEVHFPPEDIVFDPNVLTIGTGMEEHAGYGIDFINACKTIKEICPYAKISGGISNLSFGFRGVTKIRESIHAVFLDNAIRQSGMDVGIVNAHEMLMYSEVDTDIRECCEDLIFNRDPEATERMLELTKREKAAIEARKKGGGGPKVVTKPWRTEIPKKRLEHALINGISEYVIEDVEAARL----ECEKPLHVIEGPLMDGMNIVGDLFGAGKMFLPQ-VIKSARVMKKAVAYLLPFMEEEKRQKAIAEGLDPDDIDEDDDSNYAGKVIMATVKGDVHDIGKNIVAVVLGCNNYK-----------VYDIGVMCSCELILEKAKEYNVDVIGLSGLITPSLDEMVTVAKQLSKAGFKQPLLIGGATTSKMHTAVKIAPNFFNKEHPVIHVLDASRSVTVVSSLLNQNKANFVEDLQEEYDEMREDYYAGLEDRNFLTFEDARKQRKVIDFDAVPPPPVPNKIGITVID-SVSLKDVVPYMDWNPFFQTWELRGRYPNRGYPKIFDDKTVGSEAKKLFDDAQKMMEEIIENGSFTLKGVVGLFPANLSEDGEDVDIFDNEADREAGKIAAKFCMLRQQAEKESDDPFLSQADFIAPK--GYADHIGFFAVSCFGCEALCNKYEADQDDFSKIMAQSLADRFVEAFAEYLHREIRIDIWGYAKGESLDEADLLKIKYDGIRPAPGYPSQPDHTEKATMWDIVKVQEHAGIELSESLSMMPAASVSALVFAHPQSEYFAVGQIGKDQVDSYAERKKMDIELCERWLSPILNYDRD 1360