mRNA_E-siliculosus-1a_M_contig93.17325.1 (mRNA) Ectocarpus siliculosus Ec864m_EcPH12_78m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_M_contig93.17325.1
Unique NamemRNA_E-siliculosus-1a_M_contig93.17325.1
TypemRNA
OrganismEctocarpus siliculosus Ec864m_EcPH12_78m male (Ectocarpus siliculosus Ec864m_EcPH12_78m male)
Homology
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17325.1 vs. uniprot
Match: D7G906_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G906_ECTSI)

HSP 1 Score: 247 bits (630), Expect = 1.110e-82
Identity = 126/130 (96.92%), Postives = 127/130 (97.69%), Query Frame = 2 
Query:    2 MWAAFARNPRLCVPAAVKACAEFPLRATTVAGSDSRARYWPLEQRRGLSSGSDSPREDAIASILESKIEASKAVVHDISGGCGSMYKIEVESPLFRGKGLVAQHRMVKAALATEIADMHGLTLLTRPSPP 391
            MWAAFARNPRLCVPAAVKACAEFPLRA  VAGSDSRA YWPLEQRRGLS+GSDSPREDAIASILESKIEASKAVVHDISGGCGSMYKIEVESPLFRGKGLVAQHRMVKAALATEIADMHGLTLLTRPSPP
Sbjct:    1 MWAAFARNPRLCVPAAVKACAEFPLRAIAVAGSDSRACYWPLEQRRGLSNGSDSPREDAIASILESKIEASKAVVHDISGGCGSMYKIEVESPLFRGKGLVAQHRMVKAALATEIADMHGLTLLTRPSPP 130
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17325.1 vs. uniprot
Match: A0A7S2CEL3_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2CEL3_9STRA)

HSP 1 Score: 92.8 bits (229), Expect = 1.110e-21
Identity = 46/78 (58.97%), Postives = 56/78 (71.79%), Query Frame = 2 
Query:  155 SDSPREDAIASILESKIEASKAVVHDISGGCGSMYKIEVESPLFRGKGLVAQHRMVKAALATEIADMHGLTLLTRPSP 388
            S+ P+ED +   L + +E +  +V D+SGGCGSMYKIEVESPLFRG  LV QHRMV   L  EI +MHGLT+ TR SP
Sbjct:   53 SEVPKEDLMKEALIAALEPTDVLVEDVSGGCGSMYKIEVESPLFRGVNLVKQHRMVTDTLKKEIGEMHGLTINTRRSP 130
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17325.1 vs. uniprot
Match: A0A4D9DBR1_9STRA (Uncharacterized protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9DBR1_9STRA)

HSP 1 Score: 89.4 bits (220), Expect = 2.220e-20
Identity = 41/72 (56.94%), Postives = 54/72 (75.00%), Query Frame = 2 
Query:  167 REDAIASILESKIEASKAVVHDISGGCGSMYKIEVESPLFRGKGLVAQHRMVKAALATEIADMHGLTLLTRP 382
            RED + ++L    + +  ++ D+SGGCG+MY +EVES LFRGK LVAQHRMV   LA EI++MHGL+L TRP
Sbjct:   52 REDRMRALLSEHFKPTHLLIQDVSGGCGAMYNMEVESELFRGKALVAQHRMVNQVLAKEISEMHGLSLKTRP 123
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17325.1 vs. uniprot
Match: A0A836CI04_9STRA (Bola protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CI04_9STRA)

HSP 1 Score: 87.8 bits (216), Expect = 6.620e-20
Identity = 42/74 (56.76%), Postives = 55/74 (74.32%), Query Frame = 2 
Query:  167 REDAIASILESKIEASKAVVHDISGGCGSMYKIEVESPLFRGKGLVAQHRMVKAALATEIADMHGLTLLTRPSP 388
            +E A+  +L  +++A+K  V DISGGCGSMY I +ESPLF+G  LV QH++VK+ L  EIA MHGLTL T+ SP
Sbjct:   40 QEKAMEKLLGKELKATKVTVEDISGGCGSMYSIAIESPLFKGLSLVKQHQLVKSTLKKEIAAMHGLTLQTKVSP 113
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17325.1 vs. uniprot
Match: A0A1V9Y5G4_9STRA (Uncharacterized protein n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9Y5G4_9STRA)

HSP 1 Score: 82.8 bits (203), Expect = 4.060e-18
Identity = 42/72 (58.33%), Postives = 49/72 (68.06%), Query Frame = 2 
Query:  161 SPREDAIASILESKIEASKAVVHDISGGCGSMYKIEVESPLFRGKGLVAQHRMVKAALATEIADMHGLTLLT 376
            S  E+ +A  L  ++ A+   V DISGGCGSMYKIEV SP F GK +VAQHR+V   L  EIA MHGLTL T
Sbjct:   16 SAAEEKMAVALRQELSATHVKVDDISGGCGSMYKIEVASPTFEGKNVVAQHRLVNQVLKEEIAGMHGLTLKT 87
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17325.1 vs. uniprot
Match: A0A8K0AJV7_ANDGO (Mitochondrial BolA domain-containing protein n=1 Tax=Andalucia godoyi TaxID=505711 RepID=A0A8K0AJV7_ANDGO)

HSP 1 Score: 82.4 bits (202), Expect = 5.020e-18
Identity = 41/85 (48.24%), Postives = 57/85 (67.06%), Query Frame = 2 
Query:  137 RGLSSGSDSPREDAIASILESKIEASKAVVHDISGGCGSMYKIEVESPLFRGKGLVAQHRMVKAALATEIADMHGLTLLTRPSPP 391
            R LS+ ++ P E +I+ IL S ++ SK  V DISGGCG  + + VESP+FRGK +V QHRMV + L  E+  MH L + T+P+ P
Sbjct:    8 RLLSTAAEGPTEASISKILRSALQPSKLNVQDISGGCGVSFAVVVESPMFRGKTIVEQHRMVNSVLKQEMEVMHALRISTKPTSP 92
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17325.1 vs. uniprot
Match: A0A369SM06_9METZ (BolA-like protein 3 n=1 Tax=Trichoplax sp. H2 TaxID=287889 RepID=A0A369SM06_9METZ)

HSP 1 Score: 84.0 bits (206), Expect = 6.860e-18
Identity = 52/111 (46.85%), Postives = 66/111 (59.46%), Query Frame = 2 
Query:   65 EFPLRATTVAGSDSRARYWPL----EQRRGLSSGSD---SPREDAIASILESKIEASKAVVHDISGGCGSMYKIEVESPLFRGKGLVAQHRMVKAALATEIADMHGLTLLT 376
            E P+   T+    +R   W L      RR LSS  D   S  E  I+SIL+  +  S   V DISGGCGSMY+I ++S  F+GK ++ QHRMVK ALA EI +MHGLT+ T
Sbjct:   50 ELPINTNTLRSESNR---WLLLANNVSRRCLSSQPDLKQSKGEQHISSILKDTVNPSYLEVRDISGGCGSMYEILIQSDNFKGKSIIQQHRMVKEALAEEIKNMHGLTIRT 157
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17325.1 vs. uniprot
Match: A0A7S2H125_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2H125_9STRA)

HSP 1 Score: 82.0 bits (201), Expect = 1.120e-17
Identity = 45/87 (51.72%), Postives = 58/87 (66.67%), Query Frame = 2 
Query:  137 RGLSSGSDSP---REDAIASILESKIEASKAVVHDISGGCGSMYKIEVESPLFRGKGLVAQHRMVKAALATEIADMHGLTLLTRPSP 388
            R LS GS+S    +E+++   L   + AS   V DISGGCG+MYK+EVESP FRG  ++ QHR+V   L  EI +MHGLTL TR +P
Sbjct:   25 RILSIGSESDGVSKEESMRLALSQALNASFVSVDDISGGCGAMYKLEVESPDFRGLNMLKQHRLVTQVLKKEIGEMHGLTLQTRVTP 111
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17325.1 vs. uniprot
Match: A0A067C2D2_SAPPC (Uncharacterized protein n=2 Tax=Saprolegnia TaxID=4769 RepID=A0A067C2D2_SAPPC)

HSP 1 Score: 80.5 bits (197), Expect = 3.440e-17
Identity = 44/80 (55.00%), Postives = 52/80 (65.00%), Query Frame = 2 
Query:  137 RGLSSGSDSPREDAIASILESKIEASKAVVHDISGGCGSMYKIEVESPLFRGKGLVAQHRMVKAALATEIADMHGLTLLT 376
            RGLS+ +    E+ +A+ L   + A+   V DISGGCGSMYKIEV SP F GK  VAQHR+V   L  EI  MHGLTL T
Sbjct:   16 RGLSTTA----EEKMATSLRQALSATHVKVDDISGGCGSMYKIEVASPTFEGKNRVAQHRLVNEVLKEEIGGMHGLTLKT 91
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BLAST of mRNA_E-siliculosus-1a_M_contig93.17325.1 vs. uniprot
Match: B3S7G9_TRIAD (Uncharacterized protein n=1 Tax=Trichoplax adhaerens TaxID=10228 RepID=B3S7G9_TRIAD)

HSP 1 Score: 80.1 bits (196), Expect = 7.240e-17
Identity = 39/69 (56.52%), Postives = 50/69 (72.46%), Query Frame = 2 
Query:  170 EDAIASILESKIEASKAVVHDISGGCGSMYKIEVESPLFRGKGLVAQHRMVKAALATEIADMHGLTLLT 376
            E  I+SIL+  +  S   V DISGGCGSMY+I ++S  F+GK ++ QHRMVK ALA EI +MHGLT+ T
Sbjct:   45 EQHISSILKDTVNPSYLEVRDISGGCGSMYEILIQSDNFKGKSIIQQHRMVKEALAEEIKNMHGLTIRT 113
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_M_contig93.17325.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G906_ECTSI1.110e-8296.92Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A7S2CEL3_9STRA1.110e-2158.97Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
A0A4D9DBR1_9STRA2.220e-2056.94Uncharacterized protein n=1 Tax=Nannochloropsis sa... [more]
A0A836CI04_9STRA6.620e-2056.76Bola protein n=1 Tax=Tribonema minus TaxID=303371 ... [more]
A0A1V9Y5G4_9STRA4.060e-1858.33Uncharacterized protein n=1 Tax=Achlya hypogyna Ta... [more]
A0A8K0AJV7_ANDGO5.020e-1848.24Mitochondrial BolA domain-containing protein n=1 T... [more]
A0A369SM06_9METZ6.860e-1846.85BolA-like protein 3 n=1 Tax=Trichoplax sp. H2 TaxI... [more]
A0A7S2H125_9STRA1.120e-1751.72Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A067C2D2_SAPPC3.440e-1755.00Uncharacterized protein n=2 Tax=Saprolegnia TaxID=... [more]
B3S7G9_TRIAD7.240e-1756.52Uncharacterized protein n=1 Tax=Trichoplax adhaere... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_M_contig93contigE-siliculosus-1a_M_contig93:15100..16752 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a male vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec864m_EcPH12_78m male2021-02-24
Properties
Property NameValue
Start1
Stop1
Cds size393
Model size394
Exons3
Ec32 orthologEc-15_002390.1
Ec32 ortholog descriptionBolA protein
Hectar predicted targeting categorymitochondrion
BRITEko00000,ko03029
Best eggNOG OGNA|NA|NA
Best tax levelEukaryota
COG Functional cat.T
EggNOG OGsCOG0271@1,KOG3348@2759
EggNOG free text desc.protein maturation by iron-sulfur cluster transfer
GOsGO:0000981,GO:0003674,GO:0003700,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005759,GO:0006355,GO:0006357,GO:0006807,GO:0008150,GO:0008152,GO:0009889,GO:0010467,GO:0010468,GO:0010556,GO:0019219,GO:0019222,GO:0019538,GO:0031323,GO:0031326,GO:0031974,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0044238,GO:0044422,GO:0044424,GO:0044429,GO:0044444,GO:0044446,GO:0044464,GO:0050789,GO:0050794,GO:0051171,GO:0051252,GO:0051604,GO:0060255,GO:0065007,GO:0070013,GO:0071704,GO:0080090,GO:0097428,GO:0106035,GO:0140110,GO:1901564,GO:1903506,GO:2000112,GO:2001141
KEGG koko:K22075
Preferred nameBOLA3
Seed eggNOG ortholog2880.D7G906
Seed ortholog evalue2.8e-64
Seed ortholog score251.1
Taxonomic scopeEukaryota
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_M_contig93.17325.1prot_E-siliculosus-1a_M_contig93.17325.1Ectocarpus siliculosus Ec864m_EcPH12_78m malepolypeptideE-siliculosus-1a_M_contig93 15101..16752 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622936472.2349324-UTR-E-siliculosus-1a_M_contig93:15099..151001622936472.2349324-UTR-E-siliculosus-1a_M_contig93:15099..15100Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig93 15100..15100 +
1696251094.2543614-UTR-E-siliculosus-1a_M_contig93:15099..151001696251094.2543614-UTR-E-siliculosus-1a_M_contig93:15099..15100Ectocarpus siliculosus Ec864m_EcPH12_78m maleUTRE-siliculosus-1a_M_contig93 15100..15100 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622936472.262878-CDS-E-siliculosus-1a_M_contig93:15100..152041622936472.262878-CDS-E-siliculosus-1a_M_contig93:15100..15204Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig93 15101..15204 +
1696251094.2695575-CDS-E-siliculosus-1a_M_contig93:15100..152041696251094.2695575-CDS-E-siliculosus-1a_M_contig93:15100..15204Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig93 15101..15204 +
1622936472.281922-CDS-E-siliculosus-1a_M_contig93:15703..157931622936472.281922-CDS-E-siliculosus-1a_M_contig93:15703..15793Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig93 15704..15793 +
1696251094.2862592-CDS-E-siliculosus-1a_M_contig93:15703..157931696251094.2862592-CDS-E-siliculosus-1a_M_contig93:15703..15793Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig93 15704..15793 +
1622936472.2984576-CDS-E-siliculosus-1a_M_contig93:16553..167521622936472.2984576-CDS-E-siliculosus-1a_M_contig93:16553..16752Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig93 16554..16752 +
1696251094.3007536-CDS-E-siliculosus-1a_M_contig93:16553..167521696251094.3007536-CDS-E-siliculosus-1a_M_contig93:16553..16752Ectocarpus siliculosus Ec864m_EcPH12_78m maleCDSE-siliculosus-1a_M_contig93 16554..16752 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_M_contig93.17325.1

>prot_E-siliculosus-1a_M_contig93.17325.1 ID=prot_E-siliculosus-1a_M_contig93.17325.1|Name=mRNA_E-siliculosus-1a_M_contig93.17325.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=polypeptide|length=131bp
MWAAFARNPRLCVPAAVKACAEFPLRATTVAGSDSRARYWPLEQRRGLSS
GSDSPREDAIASILESKIEASKAVVHDISGGCGSMYKIEVESPLFRGKGL
VAQHRMVKAALATEIADMHGLTLLTRPSPP*
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mRNA from alignment at E-siliculosus-1a_M_contig93:15100..16752+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_M_contig93.17325.1 ID=mRNA_E-siliculosus-1a_M_contig93.17325.1|Name=mRNA_E-siliculosus-1a_M_contig93.17325.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=mRNA|length=1653bp|location=Sequence derived from alignment at E-siliculosus-1a_M_contig93:15100..16752+ (Ectocarpus siliculosus Ec864m_EcPH12_78m male)
CATGTGGGCCGCCTTTGCCAGAAATCCTCGGCTTTGTGTGCCGGCAGCGG
TGAAGGCCTGCGCTGAGTTTCCATTGAGAGCTACTACTGTGGCTGGATCG
GACAGGTGAGCTGGCACCTCAAACAGATGGACTTATTTTATGCGTAGCTC
CCGCTTGAGGCCCCTTTAGCCGGCAGCGGTGAAGGCCTGCGCTGAGTTTC
CATTGAGAGCTACTACTGTGGCTGGATCGGACAGGTGAGCTGGCACCTCA
AACAGATGGACTTATTTTATGCGTAGCTCCCGCTTGAGGCCCCTTTACTG
CTAAATAAGTGTGATGTACTCTCTACGTAATAGATGCTGCATTACCCTAT
GCAACACAAGCGTTGCTCGGCAAACGTGTACCTCTTGCGGCTGTTCACGC
ACGGCTGCCCTGCAGGACGCATGAGAAATCGCCATGTTACCATCCTTTTT
TGACGAGTTCGTGCGAACCTATTGTGTACCCGTACAGCAGTCTTGCCCTT
CTTGAACCAAAGTTGCGAATTACGGCTGTAATACTGGTTCTGGGCCTCGC
GCACTGACTCTCTCGCCTGTGCTGTACGCCTAACTCGGTCACCCTTCGGT
TTAGCCGAGCGCGCTATTGGCCACTCGAGCAGCGACGCGGCCTCAGCAGC
GGTTCGGACAGTCCAAGAGAAGATGCGATCGCTTCGATACTTGAGTAAGT
AAGCAGCTCGTGACTCGATGTATTTCAGCATACAGCCATGTACAGCAGGA
TTCTCTTGTAGAAAAGTACATCTCACACAGGGCACGATATACCTTCATAC
AACACAGGTGTCCCTATCCTACTGCATTTCACATGCACTTTCCTAGACAG
AGCTCTTGGTACGTGTTATGTAACATATTATCGATAGCCTCCAACAAAAA
TCCCCTCAAGTGTGCGGGAAAAAATCGTATTGTTTATTATTGTGCCCTTC
ATATCATGAGGTGTGTGCGTACAGTAGGTTGGAGTATTCTTGTTTGTTTT
GGGACGTTCCCAACCTCTGGTTATTTGCCTGGTGGAGATTTTTTTTTGCC
AATCTTCAGGATCTCCGCCCGGGACGAACACACTACAATCGACGATCTCC
TCTTGCCGGCAATTGGCCAATCGTTGCTGTTATTTGTGGCTCGCATGTTG
CTACTTTCCGTGAACATATTTCGGCCCATGGCAAGCCTGTGTGTGTTGTG
GTTTCCGTCTCTGATTCCGCTACACGCCCCAGAGGACGTTGCCCGCCTCT
GGGTGTTTCATCTGTCTCAAGGTCCCCCCCCTTTGCTTATTTAGCGCCGG
GGTGGTATTTTACAATTCGTTACTGTTGCCCGGGGGCTATGGCAGAGGAT
TACAGTGGTCGGTCCTTTTCAAAATGATCGTCCTGTCGAAGGGACGTAAG
TGTTGCTCAATCCAACACGTGCTTTGCCTGCCTACGCTGTGCCTTGTTGC
GCAGGTCGAAGATAGAGGCATCGAAAGCTGTCGTGCATGATATCTCAGGG
GGTTGCGGGAGCATGTACAAAATCGAGGTCGAGTCACCGTTGTTTCGGGG
CAAGGGTCTGGTGGCGCAACACCGCATGGTGAAGGCCGCTCTCGCGACCG
AGATCGCGGACATGCATGGGCTAACGCTGCTCACGAGGCCATCGCCACCC
TAG
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Coding sequence (CDS) from alignment at E-siliculosus-1a_M_contig93:15100..16752+

>mRNA_E-siliculosus-1a_M_contig93.17325.1 ID=mRNA_E-siliculosus-1a_M_contig93.17325.1|Name=mRNA_E-siliculosus-1a_M_contig93.17325.1|organism=Ectocarpus siliculosus Ec864m_EcPH12_78m male|type=CDS|length=786bp|location=Sequence derived from alignment at E-siliculosus-1a_M_contig93:15100..16752+ (Ectocarpus siliculosus Ec864m_EcPH12_78m male)
ATGTGGGCCGCCTTTGCCAGAAATCCTCGGCTTTGTGTGCCGGCAGCGGT
GAAGGCCTGCGCTGAGTTTCCATTGAGAGCTACTACTGTGGCTGGATCGG
ACAGATGTGGGCCGCCTTTGCCAGAAATCCTCGGCTTTGTGTGCCGGCAG
CGGTGAAGGCCTGCGCTGAGTTTCCATTGAGAGCTACTACTGTGGCTGGA
TCGGACAGCCGAGCGCGCTATTGGCCACTCGAGCAGCGACGCGGCCTCAG
CAGCGGTTCGGACAGTCCAAGAGAAGATGCGATCGCTTCGATACTTGACC
GAGCGCGCTATTGGCCACTCGAGCAGCGACGCGGCCTCAGCAGCGGTTCG
GACAGTCCAAGAGAAGATGCGATCGCTTCGATACTTGAGTCGAAGATAGA
GGCATCGAAAGCTGTCGTGCATGATATCTCAGGGGGTTGCGGGAGCATGT
ACAAAATCGAGGTCGAGTCACCGTTGTTTCGGGGCAAGGGTCTGGTGGCG
CAACACCGCATGGTGAAGGCCGCTCTCGCGACCGAGATCGCGGACATGCA
TGGGCTAACGCTGCTCACGAGGCCATCGCCACCCTAGGTCGAAGATAGAG
GCATCGAAAGCTGTCGTGCATGATATCTCAGGGGGTTGCGGGAGCATGTA
CAAAATCGAGGTCGAGTCACCGTTGTTTCGGGGCAAGGGTCTGGTGGCGC
AACACCGCATGGTGAAGGCCGCTCTCGCGACCGAGATCGCGGACATGCAT
GGGCTAACGCTGCTCACGAGGCCATCGCCACCCTAG
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