mRNA_E-siliculosus-1a_M_contig8.16194.1 (mRNA) Ectocarpus siliculosus Ec864m_EcPH12_78m male

BLAST of mRNA_E-siliculosus-1a_M_contig8.16194.1 vs. uniprot
Match: A0A6H5KJ16_9PHAE (DNA-directed RNA polymerase subunit n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KJ16_9PHAE)

HSP 1 Score: 3318 bits (8604), Expect = 0.000e+0
Identity = 1726/1890 (91.32%), Postives = 1781/1890 (94.23%), Query Frame = 2

Query:  149 MSHDRTVIRHAVEEIGFGFLTDDDIRRLSVKRLTSPVTFDTLNNPLPGGLYDPVLGPVDKMMMCETCGQDQKNCPGHMGHIELAVSVYHPILFGALYKLLRAKCFSCHNLRLSKSKTRVAAVKMMLVDAGRAQEALELDATLLGYLKDGARDHDVLEGNTEDTLVSATKYRDHVLGCLEEDLAGAPRAEWCGGHNRQLRRQLAESLIKAMGACKRCENCGAFSPNIRKDGSNKLFQVPLSDKNRMANQSCNTQIVSAVQRRDPGKPNEDXXXXXXXXXXXXXXXXXXXXXXXDDVGGSGPSSKVVGVPLAAAQAAAGDPAAKTGAQAHKFMPPIEVELQMQQLWKQESSTLDLIFVSGRRTRGVPGDVNGAPSSSSQNADGLSGGGIADGYRLFFVRALAVPPPRFRPPMNMGDMIAEHPQNVYLTKVLNLNESLKNADSLKGEDLASVLTTWVELQTTVNCYMDSSKDPRGLKDTPPGLRQVLEKKEGLFRKHMMGKRVNYACRSVISPDPYLGTTEIGIPLRFAKELTYPQPVADWNVEAMRQLVENGTSVYPGANFVEDSSGRMLHLDRLSDLKRRGVAARLLSSPGQKVWRHLTDGDCMLVNRQPTLHKPGIMAHRVRVLRNPGYQTIRMHYANCNTYNADFDGDEINCHLPQNELAKAEANLLAFTDEQYLVPTNGKPLRGLIQDHVDAGVKMCSKDCFFTRGEYQQLLYQALSGLPGLEIVPPSDDIATLPPAILKPQQRWTGKQVMSTILRHLTKGLPQLNLDGKSRTPKVAFGEAEQEHMIIFREGELLQGVLDKGAFGSSEFGLVHAVHEVYGSTAAGKLLTALGRVLTIFLQSSGHTCGIEDLTLTARAEGNRREIINKSLGIGQRSMRELVDNAG-EGQNAKAIANGSATEKGDEDTGEALVEAEVEGIRQKTASFLLGEDRDSRLAEIDRHMQSALAPVSSDIIKACLPGGQAKPFPHNCFSLMVLTGAKGSMVNHSQVSCALGQQALEGRRVPVMVSGKSLPSFQAFEPSPRANGFITDRFLTGIRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEELKVGYDNTVRDGEGCVHQFLYGEDGVDTTQTKYLSSKQLGFLAQNYQALRHKHGITPSFKKDAGFDSREASRAHSEIATAQRRRDDAAGAASASGAKFVAGEMVLAKRRAVLKGGEGWGKAELLAGWHSAEVVKARNAGTSAALYSVRYKDDGAMGKKMPVSAAQEGPSESFADPSPEENNESGHLSSRRRKRPRRLLRKCPGAGLPDPVLSTLSVNKDLGCVSEAFQKSLQAFLDSNPASLRGDHTKENLKAVAATDTSSPPVLGSLKEAFELLMWVKYMRCLANPGETVGSIAAQSVGEPSTQMTLNTFHLAGHGGANVTLGIPRLREIIMTAAKNLKTPSMVVPLREGADRPAAKSLALRLSRLPLSQLLDNRGGIVVRERLVKGDTGLWERHYAISLKLFPPSVINRAFDISFKELCRKISGVFVPNLLSAIANDLRRTGNKLSQTGGASVGLPSKGRGKRADGDRVEEEDDLEGERSAIAQSKAVAKKASKAEXXXXXXXXXXEQGTLKFGKQKEQSSYGGMDEEEKAIWKAMSGKEAGGGVLG--DDDHXXXXXEGREGVDGVGKDKDDDRDYFNLPNLSGARHFKGITKNKKTGEVEVTVRLPSSMRRLLMVGLAESAAAKTMVRSHKGIRGAFVVETTVDGKSGLAVQLEGSDFETVWQLKEGGVDGILNLSKLTSNDIWAVCQTYGVEAARASIVAEITGVFGAYGIAVDPRHLGLVADHMTFDGGYRPLNRAGMADFSSPCLQMSFETTTDFMAKAAVARSSDRLKSPSARIVMGRVGNFGTGMFDLRQPAEFGGVGQVGGQE 5809
            MSHDRTVIRHAVEEIGFGFLTDDDIR+LSVKR+TSPVTFDTLNNPLPGGLYDPVLGPVDKMMMC TCGQDQKNCPGHMGHIELAVSVYHPILFGAL+KLLRAKCFSCHNLRLSKSKTRVAAVKMMLVDAGRAQEALELDATLLGYLKDGA+DHDVL+GNTEDTLVSATK+RD VLGCLEEDLA APRA+WCGGHNRQLRRQLAE+LIKA+GACKRCENCGAFSPNIRKDGSNKLFQVPLSDKNRMANQ+CNTQIVSAVQRRDPGKPNEDXXXXXXXXXXXXXXXXXXXXXXXDD+ GS PSSKVVGVPLAAAQA AG+PAAK GAQ H+FMPPIEVELQMQQLWKQE STLDLIFVS RRTR VPGDV+GA SSSSQN DGL  GGI+DGYR FF+R LAVPPPRFRPPMNMGD+IAEHPQNV+LTKVLNLNESLKNADSLKG+DLASVLTTWVELQTTVNCYMDSSKDPRGLK+TPPGLRQVLEKKEGLFRKHMMGKRVNYACRSVISPDPYLGTTEIGIPLRFAKELTYPQPVADWNVEAMRQLVENGTSVYPGANFVEDSSGRMLHLDRLSDLKRRGVAARLLS PGQKVWRHL DGDCMLVNRQPTLHKPGIMAHRVRVLRNPGYQTIRMHYANCNTYNADFDGDEINCHLPQNELAKAEANLLAFTDEQYLVPTNGKPLRGLIQDHVDAGVKMCSKDCFFT+GEYQQL+YQALSGLPGLEIVPPSDDI TLPPAILKPQQRWTGKQVMSTILRHLTKGLPQLNLDGKSRTPKVAFGEAEQEH+I+FREGELLQGVLDKGAFGSSEFGLVHAVHEVYGSTAAGKLLTALGRVLTIFLQSSGHTCGIEDLTLTA AEGNRREII KSLGIGQRSMRELVD A  EGQNAKAIANG      DEDTG+ALVEA+VE IRQKTA FLLGEDRD+RLAEIDRHMQSALAPVSSDIIKACLPGGQAKPFPHNCFSLMVLTGAKGSMVNHSQVSCALGQQALEGRRVPVMVSGKSLPSFQAFEPSPRANGFITDRFLTGIRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEELKVGYDNTVRDGEGCVHQFLYGEDG+DTTQTKYLSSKQLGFLAQNYQALRHKHGITPSFK DAGFD R ASRAHSEIATAQ+R DDA+GAA AS AKFVAGE VLAKRR VL GGEGWGKAELLAGWHSAEV+K R AGT AALYSVRYKDDGAM K+MP+SA QEG SE+ +D SP EN E+GH SSRRRKRPRRLLRKCPGAGLPDPVLSTL+VNKDLGCVSEAFQKSLQAFLDSNPASLRGDHT ++ KA AAT   S P + SLKEAFELLMWVKYMRCLANPGETVGSIAAQSVGEPSTQMTLNTFHLAGHGGANVTLGIPRLREIIMTAAKNLKTPSMVVPLREGADR  A+SLA RLSRLPLSQLLDNRGGIVVRERLVK +TGLWERHY + L+LF PS+I+ AFDISFKELCRKISGVFVPNLLSAIANDLRRTGNKLSQTGGASVGLP +GRG+RA GD   EEDDLEG+ SAI QS A AKKASKAE          EQGTLKFGKQKEQSSYGGMDEEEKAIWKAMSGKEA GGVLG  D D      EG  G DGVG DK+DDRDYFNLPN+SGARH KGITKNKKTGEVEVTVRLPSSMRRLLMVGLAESAAAKTMVRSHKGIRGAFVV+ TV+GKSGLAVQLEGSDFETVWQLKEGGVDGILNL KLTSNDIWAVCQTYGVEAARASIV+EITGVFGAYGIAVDPRHLGLVADHMTFDGGYRPLNRAGMADFSSPCLQMSFETTTDFMAKAAVARSSDRLKSPSARIVMGRVG+FGTGMFDLRQPAEFGGVG   GQE
Sbjct:    1 MSHDRTVIRHAVEEIGFGFLTDDDIRKLSVKRITSPVTFDTLNNPLPGGLYDPVLGPVDKMMMCATCGQDQKNCPGHMGHIELAVSVYHPILFGALFKLLRAKCFSCHNLRLSKSKTRVAAVKMMLVDAGRAQEALELDATLLGYLKDGAKDHDVLDGNTEDTLVSATKHRDRVLGCLEEDLADAPRADWCGGHNRQLRRQLAENLIKAIGACKRCENCGAFSPNIRKDGSNKLFQVPLSDKNRMANQTCNTQIVSAVQRRDPGKPNEDXXXXXXXXXXXXXXXXXXXXXXXDDIAGSAPSSKVVGVPLAAAQADAGNPAAKRGAQFHEFMPPIEVELQMQQLWKQECSTLDLIFVSDRRTRTVPGDVSGARSSSSQNVDGLHRGGISDGYRFFFMRVLAVPPPRFRPPMNMGDLIAEHPQNVHLTKVLNLNESLKNADSLKGDDLASVLTTWVELQTTVNCYMDSSKDPRGLKETPPGLRQVLEKKEGLFRKHMMGKRVNYACRSVISPDPYLGTTEIGIPLRFAKELTYPQPVADWNVEAMRQLVENGTSVYPGANFVEDSSGRMLHLDRLSDLKRRGVAARLLSIPGQKVWRHLKDGDCMLVNRQPTLHKPGIMAHRVRVLRNPGYQTIRMHYANCNTYNADFDGDEINCHLPQNELAKAEANLLAFTDEQYLVPTNGKPLRGLIQDHVDAGVKMCSKDCFFTKGEYQQLVYQALSGLPGLEIVPPSDDITTLPPAILKPQQRWTGKQVMSTILRHLTKGLPQLNLDGKSRTPKVAFGEAEQEHVIVFREGELLQGVLDKGAFGSSEFGLVHAVHEVYGSTAAGKLLTALGRVLTIFLQSSGHTCGIEDLTLTAGAEGNRREIIKKSLGIGQRSMRELVDTASAEGQNAKAIANG------DEDTGKALVEADVEDIRQKTAFFLLGEDRDTRLAEIDRHMQSALAPVSSDIIKACLPGGQAKPFPHNCFSLMVLTGAKGSMVNHSQVSCALGQQALEGRRVPVMVSGKSLPSFQAFEPSPRANGFITDRFLTGIRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEELKVGYDNTVRDGEGCVHQFLYGEDGIDTTQTKYLSSKQLGFLAQNYQALRHKHGITPSFKNDAGFDCRNASRAHSEIATAQQRSDDASGAAGASRAKFVAGETVLAKRRTVLNGGEGWGKAELLAGWHSAEVMKVRKAGTPAALYSVRYKDDGAMAKRMPLSAPQEGSSENSSDSSPNENGENGHPSSRRRKRPRRLLRKCPGAGLPDPVLSTLNVNKDLGCVSEAFQKSLQAFLDSNPASLRGDHTNDSSKAGAATGRLSSPGVRSLKEAFELLMWVKYMRCLANPGETVGSIAAQSVGEPSTQMTLNTFHLAGHGGANVTLGIPRLREIIMTAAKNLKTPSMVVPLREGADRSTARSLARRLSRLPLSQLLDNRGGIVVRERLVKENTGLWERHYVVCLRLFRPSIISMAFDISFKELCRKISGVFVPNLLSAIANDLRRTGNKLSQTGGASVGLPPRGRGERA-GD---EEDDLEGQISAIPQSNAGAKKASKAEAEYESDDEDEEQGTLKFGKQKEQSSYGGMDEEEKAIWKAMSGKEANGGVLGEEDGDDDGNDDEGMGGADGVGNDKNDDRDYFNLPNISGARHLKGITKNKKTGEVEVTVRLPSSMRRLLMVGLAESAAAKTMVRSHKGIRGAFVVDMTVEGKSGLAVQLEGSDFETVWQLKEGGVDGILNLDKLTSNDIWAVCQTYGVEAARASIVSEITGVFGAYGIAVDPRHLGLVADHMTFDGGYRPLNRAGMADFSSPCLQMSFETTTDFMAKAAVARSSDRLKSPSARIVMGRVGSFGTGMFDLRQPAEFGGVG---GQE 1877          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16194.1 vs. uniprot
Match: D7FNY1_ECTSI (DNA-directed RNA polymerase subunit n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FNY1_ECTSI)

HSP 1 Score: 2280 bits (5909), Expect = 0.000e+0
Identity = 1169/1225 (95.43%), Postives = 1189/1225 (97.06%), Query Frame = 2

Query:  149 MSHDRTVIRHAVEEIGFGFLTDDDIRRLSVKRLTSPVTFDTLNNPLPGGLYDPVLGPVDKMMMCETCGQDQKNCPGHMGHIELAVSVYHPILFGALYKLLRAKCFSCHNLRLSKSKTRVAAVKMMLVDAGRAQEALELDATLLGYLKDGARDHDVLEGNTEDTLVSATKYRDHVLGCLEEDLAGAPRAEWCGGHNRQLRRQLAESLIKAMGACKRCENCGAFSPNIRKDGSNKLFQVPLSDKNRMANQSCNTQIVSAVQRRDPGKPNEDXXXXXXXXXXXXXXXXXXXXXXXDDVGGSGPSSKVVGVPLAAAQAAAGDPAAKTGAQAHKFMPPIEVELQMQQLWKQESSTLDLIFVSGRRTRGVPGDVNGAPSSSSQNADGLSGGGIADGYRLFFVRALAVPPPRFRPPMNMGDMIAEHPQNVYLTKVLNLNESLKNADSLKGEDLASVLTTWVELQTTVNCYMDSSKDPRGLKDTPPGLRQVLEKKEGLFRKHMMGKRVNYACRSVISPDPYLGTTEIGIPLRFAKELTYPQPVADWNVEAMRQLVENGTSVYPGANFVEDSSGRMLHLDRLSDLKRRGVAARLLSSPGQKVWRHLTDGDCMLVNRQPTLHKPGIMAHRVRVLRNPGYQTIRMHYANCNTYNADFDGDEINCHLPQNELAKAEANLLAFTDEQYLVPTNGKPLRGLIQDHVDAGVKMCSKDCFFTRGEYQQLLYQALSGLPGLEIVPPSDDIATLPPAILKPQQRWTGKQVMSTILRHLTKGLPQLNLDGKSRTPKVAFGEAEQEHMIIFREGELLQGVLDKGAFGSSEFGLVHAVHEVYGSTAAGKLLTALGRVLTIFLQSSGHTCGIEDLTLTARAEGNRREIINKSLGIGQRSMRELVDN-AGEGQNAKAIANGSATEKGDEDTGEALVEAEVEGIRQKTASFLLGEDRDSRLAEIDRHMQSALAPVSSDIIKACLPGGQAKPFPHNCFSLMVLTGAKGSMVNHSQVSCALGQQALEGRRVPVMVSGKSLPSFQAFEPSPRANGFITDRFLTGIRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEELKVGYDNTVRDGEGCVHQFLYGEDGVDTTQTKYLSSKQLGFLAQNYQALRHKHGITPSFKKDAGFDSREASRAHSEIATAQRRRDDAAGAASASGAKFVAGEMVLAKRRAVLKGGEGWGKAELLAGWHSAEVVKARNAGTSAALYSVR 3820
            MSHDRTVIRHAVEEIGFGFLTDDDIRRLSVKR+TSPVTFDTLNNPLPGGLYDPVLGPVDKMMMCETCGQDQKNCPGHMGHIELAVSVYHPILFGALYKLLRAKCFSCHNLRLSKSKTRVAAVKMMLVDAGRAQEALELDATLLGYLKDGARDHDVL+GNTEDTLVSATKYRDHVLGCLEEDLAGAPRAEWCGGHNRQLRRQLAE+LIKAMGACKRCENCGAFSPNIRKDGSNKLFQVPLSDKNRMANQSCNTQIVSAVQRRDPGKPNEDXXXXXXXXXXXXXXXXXX     DDVGGS PSSKVVGV LAAAQAAAGDPA KTGAQAHKFMPPIEVELQMQQLWK ESS LDLIFVSGRRTR  PGDVNG PS S QNA+ L+GG I+DGYRLFFVRALAVPPPRFRPPMNMGDMIAEHPQNVYLTKVLNLNESLKNA SLKG+DLASVLTTWVELQTTVNCYMDSSKDPRGLKDTPPGLRQVLEKKEGLFRKHMMGKRVNYACRSVISPDPY+GTTEIGIPLRFAKELTYPQPVADWNVEAMRQLVENGTSVYPGANFVEDSSGRMLHLDRLSDLKRRGVAARLLSSPGQKVWRHLTDGDCMLVNRQPTLHKPGIMAHRVRVLRNPGYQTIRMHYANCNTYNADFDGDEINCHLPQNELAKAEANLLAFTDEQYLVPTNGKPLRGLIQDHVDAGVKMCSKDCFFTRGEYQQLLYQALSGLPGLEIVPPSDDI TLPPAILKPQQRWTGKQVMSTILRHLTKGLPQLNLDGKSRTPKVAFGEAEQEH+I+FREGELLQGVLDKGAFGSSEFGLVHAVHEVYGSTAAGKLLTALGRVLTIFLQSSGHTCGIEDLTLT RAEGNRREIINKSLGIGQRSMRELV+  A EGQ+AKAIANG+A E+G EDT +ALVEAEVE IRQKTASFLLGEDRD+RLAEIDRHMQSALAPVSSDIIKACLPGGQAKPFPHNCFSLMVLTGAKGSMVNHSQVSCALGQQALEGRRVPVMVSGKSLPSFQAFEPSPRANGFITDRFLTGIRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEELKVGYDNTVRDGEGCVHQFLYGEDGVDTTQTKYLSSKQLGFLAQNYQALRHKHGITPSFKKD+GFDSR+ASRAHSEIAT  +RRDDAA   S+S AKFVAGEMVLAKRRAVLK GEGWGKAELLAGWHSAEVVK RNAGT AALYSVR
Sbjct:    1 MSHDRTVIRHAVEEIGFGFLTDDDIRRLSVKRITSPVTFDTLNNPLPGGLYDPVLGPVDKMMMCETCGQDQKNCPGHMGHIELAVSVYHPILFGALYKLLRAKCFSCHNLRLSKSKTRVAAVKMMLVDAGRAQEALELDATLLGYLKDGARDHDVLDGNTEDTLVSATKYRDHVLGCLEEDLAGAPRAEWCGGHNRQLRRQLAENLIKAMGACKRCENCGAFSPNIRKDGSNKLFQVPLSDKNRMANQSCNTQIVSAVQRRDPGKPNEDXXXXXXXXXXXXXXXXXXNLED-DDVGGSAPSSKVVGVSLAAAQAAAGDPATKTGAQAHKFMPPIEVELQMQQLWKLESSVLDLIFVSGRRTRSAPGDVNGPPSFSLQNANDLAGGSISDGYRLFFVRALAVPPPRFRPPMNMGDMIAEHPQNVYLTKVLNLNESLKNAASLKGKDLASVLTTWVELQTTVNCYMDSSKDPRGLKDTPPGLRQVLEKKEGLFRKHMMGKRVNYACRSVISPDPYVGTTEIGIPLRFAKELTYPQPVADWNVEAMRQLVENGTSVYPGANFVEDSSGRMLHLDRLSDLKRRGVAARLLSSPGQKVWRHLTDGDCMLVNRQPTLHKPGIMAHRVRVLRNPGYQTIRMHYANCNTYNADFDGDEINCHLPQNELAKAEANLLAFTDEQYLVPTNGKPLRGLIQDHVDAGVKMCSKDCFFTRGEYQQLLYQALSGLPGLEIVPPSDDITTLPPAILKPQQRWTGKQVMSTILRHLTKGLPQLNLDGKSRTPKVAFGEAEQEHVIVFREGELLQGVLDKGAFGSSEFGLVHAVHEVYGSTAAGKLLTALGRVLTIFLQSSGHTCGIEDLTLTGRAEGNRREIINKSLGIGQRSMRELVETVAAEGQDAKAIANGAAAEEGHEDTSKALVEAEVESIRQKTASFLLGEDRDTRLAEIDRHMQSALAPVSSDIIKACLPGGQAKPFPHNCFSLMVLTGAKGSMVNHSQVSCALGQQALEGRRVPVMVSGKSLPSFQAFEPSPRANGFITDRFLTGIRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEELKVGYDNTVRDGEGCVHQFLYGEDGVDTTQTKYLSSKQLGFLAQNYQALRHKHGITPSFKKDSGFDSRDASRAHSEIATCLQRRDDAAANPSSSCAKFVAGEMVLAKRRAVLKRGEGWGKAELLAGWHSAEVVKVRNAGTPAALYSVR 1224          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16194.1 vs. uniprot
Match: A0A836C8M0_9STRA (DNA-directed RNA polymerase subunit n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C8M0_9STRA)

HSP 1 Score: 1706 bits (4417), Expect = 0.000e+0
Identity = 993/1897 (52.35%), Postives = 1223/1897 (64.47%), Query Frame = 2

Query:  149 MSHDRTVIRHAVEEIGFGFLTDDDIRRLSVKRLTSPVTFDTLNNPLPGGLYDPVLGPVDKMMMCETCGQDQKNCPGHMGHIELAVSVYHPILFGALYKLLRAKCFSCHNLRLSKSKTRVAAVKMMLVDAGRAQEALELDATLLGYLKDGARDHDVLEGNTEDTLVSATKYRDHVLGCLEEDLAGAPRAEWCGGHNRQLRRQLAESLIKAMGACKRCENCGAFSPNIRKDGSNKLFQVPLSDKNRMANQSCNTQIVSAVQR-RDPG----KPNEDXXXXXXXXXXXXXXXXXXXXXXXDDVGGSGPSSKVVGVPLAAAQAAAGDPAAKTGAQAHKFMPPIEVELQMQQLWKQESSTLDLIFVSGRRTRGVPGDVNGAPSSSSQNADGLSGGGIADGYRLFFVRALAVPPPRFRPPMNMGDMIAEHPQNVYLTKVLNLNESLKNADSLKGEDLASVLTTWVELQTTVNCYMDSSKDPR-GLKDTPPGLRQVLEKKEGLFRKHMMGKRVNYACRSVISPDPYLGTTEIGIPLRFAKELTYPQPVADWNVEAMRQLVENGTSVYPGANFVEDSSGRMLHLDRLSDLKRRGVAARLLSSPGQKVWRHLTDGDCMLVNRQPTLHKPGIMAHRVRVLRNPGYQTIRMHYANCNTYNADFDGDEINCHLPQNELAKAEANLLAFTDEQYLVPTNGKPLRGLIQDHVDAGVKMCSKDCFFTRGEYQQLLYQALSGLPGLEIVPPSDDIATLPPAILKPQQRWTGKQVMSTILRHLTKGLPQLNLDGKSRTPKVAFGEAEQEHMIIFREGELLQGVLDKGAFGSSEFGLVHAVHEVYGSTAAGKLLTALGRVLTIFLQSSGHTCGIEDLTLTARAEGNRREIINKSLGIGQRSMRELVDNAGEGQNAKAIANGSATEKGDEDTGEALVEAEVEGIRQKTASFLLGEDRDSRLAEIDRHMQSALAPVSSDIIKACLPGGQAKPFPHNCFSLMVLTGAKGSMVNHSQVSCALGQQALEGRRVPVMVSGKSLPSFQAFEPSPRANGFITDRFLTGIRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEELKVGYDNTVRDGEGCVHQFLYGEDGVDTTQTKYLSSK--QLGFLAQNYQALRHKHGITPSFKKDAGFDSREASRAHSEIATAQRRRDDAAGAASASGAKFVAGEMVLAKRRAVLKGGEGWGKAELLAGWHSAEVVKARNAGTSAAL-----YSVRYKDDGAMGKKMPVSAAQEGPSESFADPSPEENNESGHLSSRRRKRPRRLLRKCPGAGLPDPVLSTLSVNKDLGCVSEAFQKSLQAFLDSNPASLRGDHTKENLKAVAATDTSSPPVLGSLKEAFELLMWVKYMRCLANPGETVGSIAAQSVGEPSTQMTLNTFHLAGHGGANVTLGIPRLREIIMTAAKNLKTPSMVVPLREGADRPAAKSLALRLSRLPLSQLLDNRGG-IVVRERLVKGDTGLWERHYAISLKLFPPSVINRAFDISFKELCRKISGVFVPNLLSAIANDLRRTGNKLSQTGGASVGLPSKGRGKRADGDRVEEEDDLEGERSAIAQSKAVAKKASKAEXXXXXXXXXXEQGTLKFGKQKEQSSYGGMDEEEKAIWKAMSGKEAGGGVLGDDDHXXXXXEGREGVDG----VGKDKDDDRDYFNLPN-LSGARHFKGITKNKKTGEVEVTVRLPSSMRRLLMVGLAESAAAKTMVRSHKGIRGAFVVETTVDGKSGLAVQLEGSDFETVWQL--KEGGVDGILNLSKLTSNDIWAVCQTYGVEAARASIVAEITGVFGAYGIAVDPRHLGLVADHMTFDGGYRPLNRAGMADFSSPCLQMSFETTTDFMAKAAVARSSDRLKSPSARIVMGRVGNFGTGMFDLRQPAE 5776
            MSHDR VIRH VEE+ FGFL+ D+IR +SVK++TSP+TFD LNNPLPGGLYDP LGP  +  +C TCGQD  +CPGH+GHIELA  VYHP+LF  L+KLL+ KCFSCH LR++    R+ AVKM L DAG    A  LDA + G    G  + +  EG+ ED L    +    +L                       RRQ+A+ +IKA+GACK+C+NCGAFSP +RKDG  KLF  PLSDK + AN     ++ SA+Q  +D      +P  D  XXXXXXXXXXXXXXXXXXXXX                                 +  +++ P+E + QMQ LW++E   L L+F    +T   P  +  AP   +        G    G+RLFF++ +AVPPPRFRPPM M DM+AEHPQN YLTK                                +NC++DSSKD     KD  PGLRQ+LEKKEGLFRKHMMGKRVN++CRSVISPDPY+GTTEIGIP RFAK L +PQPVA WNVE +R LVENG   YPGAN++ED++G++++L R S ++R+G+AARLLS PGQKVWRHL D D MLVNRQPTLHKPGIMAH  R+LRNP YQTIRMHYANCNTYNADFDGDEINCHLPQNE+A+AEAN+LAFT+EQYLVPT+GKPLRGLIQD VDAGVK+CSK  + TR ++QQL++Q         I  P   I T  PAILKP+Q W+GKQV S IL+HLT GLPQLNLD K++TP VAFGE  QEH ++ REGELLQGVLDK AFG++EFGLVH+VHE+YG+ AAG LLT+LGRVL  FLQ +GHTCGIEDLTLT  AE  RR +I                                                   IR KTA  L G D  S+   +D  M S L+ V+SDIIKACLPGGQ KPFP N FSLMVLTGAKGSMVNHSQVSC LGQQALEGRRVP+M+SGKSLPSFQAF+ +PRANGFITDRFLTGIRPQ+YYFHCMAGREGLVDTAVKTSRSGYLQRCL+KHLEELKV YD TVRDG+G V QF+YGEDG+D TQ+K++S    QL F+A+NY+A+ HK+ +         F+  +A  AH  ++ AQ + D         GA F  G+ V A+R  + KG E W +  L  GW +A +VK   + + AA      Y++RY  DG   +K+P++        + AD           L + R      L+++C      DPVLSTL+VN+DLG VSE F   L+AF+ +  +  RG       +  AA D             F ++MWVKYMR LA PGE VG+IA QSVGEPSTQMTLNTFHLAGHGGANVTLGIPRLREI+MTA++ LKTP+  VPL  G  R   ++LA  L+RLPL +LL N GG + VRE+L KGDTGLWERHY I+L+LFP   I +AF ++FK+LCR IS  FVP LL  +A +LRR G   +     S    +K            +  D EG                              QGTLKFG++KEQ+SYG MD++EK +WKAM GK  G  +  DDD      +GR   DG    +  D+ D  D+ +L   +   + F  +  NK +  V VT++ P+S+RRLLMVG+AE+AAA ++VRSH  I  +F+VE  + G++ +A+Q EG+DF T+W L  +EGG   +L+L +L SN I+ +  +YGVEA RA+I  EI  VF  YGI VD RHLGL+AD+MTF GGYR +NR GM D +SP LQMSFETT  F+ +AA+    ++LKSPSARIVMG+VG+FGTG FDL  P E
Sbjct:    1 MSHDRQVIRHKVEEVKFGFLSGDEIRAISVKQITSPLTFDALNNPLPGGLYDPALGPSQQRAVCPTCGQDHLSCPGHVGHIELAAEVYHPLLFPILFKLLKTKCFSCHKLRMNSQTCRIYAVKMALCDAGETNMARSLDAEIAG----GGAEANTDEGD-EDLLPDKAETISAILA----------------------RRQIADDVIKAIGACKKCQNCGAFSPVLRKDGHTKLFLKPLSDKMQKANNLVAAKMGSAMQAIKDKAAAANQPGNDSDXXXXXXXXXXXXXXXXXXXXXXXXXXD---------------------------EHDQYVSPLEAQAQMQLLWEKEGHLLSLLF----KTSMPPPKLLSAPQPVA--------GYDPQGFRLFFMKTIAVPPPRFRPPMVMADMVAEHPQNQYLTK------------------------------NQINCFIDSSKDTSVNAKDAAPGLRQILEKKEGLFRKHMMGKRVNHSCRSVISPDPYIGTTEIGIPERFAKVLCFPQPVAAWNVEHLRTLVENGADFYPGANYIEDAAGKLINLRRQSAVQRKGMAARLLSEPGQKVWRHLQDRDAMLVNRQPTLHKPGIMAHHARILRNPTYQTIRMHYANCNTYNADFDGDEINCHLPQNEVARAEANVLAFTNEQYLVPTDGKPLRGLIQDSVDAGVKLCSKSTWLTREDFQQLMFQ---------ITGPGTPIRTPQPAILKPKQLWSGKQVFSCILQHLTAGLPQLNLDAKTKTPAVAFGEQWQEHRVVIREGELLQGVLDKAAFGATEFGLVHSVHELYGADAAGSLLTSLGRVLVTFLQFAGHTCGIEDLTLTQSAEKERRRLIGT-------------------------------------------------IRAKTAEMLAGNDMASQAGALDGFMMSELSGVASDIIKACLPGGQEKPFPSNNFSLMVLTGAKGSMVNHSQVSCGLGQQALEGRRVPIMISGKSLPSFQAFDSNPRANGFITDRFLTGIRPQDYYFHCMAGREGLVDTAVKTSRSGYLQRCLIKHLEELKVEYDGTVRDGDGGVFQFMYGEDGIDATQSKFMSGAKPQLEFMARNYRAIVHKYDLDQGILDK--FNVEKAVEAHDAVSYAQGKDD---------GALFEVGQSVSARR--LRKGREDWCRENLRKGWFTATIVKVHASSSDAASPERTKYTLRYAADGMEARKVPLTIKLP----TAADT----------LGASRVP----LIQRCSA----DPVLSTLNVNQDLGAVSERFHARLEAFMAAE-SHRRG--LLSGRRGAAARDD------------FTVMMWVKYMRSLAAPGEAVGAIAGQSVGEPSTQMTLNTFHLAGHGGANVTLGIPRLREIVMTASRALKTPAATVPLLRGVSRAQGEALARLLTRLPLLELLLNTGGGVTVREKLGKGDTGLWERHYVIALRLFPAKKILKAFSLTFKQLCRAISQTFVPRLLRLVATELRRCGELAAARSKKSAVSKAKXXXXXXXXXXXXDSGDEEG------------------------------QGTLKFGRKKEQASYGDMDDDEKEMWKAMKGK-GGDPLNSDDDGDDASDDGRSRGDGQTTQLDGDEQDGLDFLDLSTGVKNNQMFGRMDFNKASNTVTVTLKFPASVRRLLMVGIAEAAAATSVVRSHGKIGRSFLVEQLIGGENRMALQTEGADFATLWALGPREGGT--LLDLPQLASNHIYGMLMSYGVEACRAAITKEIAAVFAVYGIGVDARHLGLIADYMTFYGGYRAMNRIGMKDIASPFLQMSFETTASFLVQAALDSKVEKLKSPSARIVMGQVGDFGTGQFDLMVPVE 1660          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16194.1 vs. uniprot
Match: K8YRY5_NANGC (DNA-directed RNA polymerase subunit n=2 Tax=Monodopsidaceae TaxID=425072 RepID=K8YRY5_NANGC)

HSP 1 Score: 1439 bits (3725), Expect = 0.000e+0
Identity = 875/1956 (44.73%), Postives = 1159/1956 (59.25%), Query Frame = 2

Query:  143 AIMSHDRTVIRHAVEEIGFGFLTDDDIRRLSVKRLTSPVTFDTLNNPLPGGLYDPVLGPVDKMMMCETCGQDQKNCPGHMGHIELAVSVYHPILFGALYKLLRAKCFSCHNLRLSKSKTRVAAVKMMLVDAGRAQEALELDATLLGYLKDGARDHDVLEGNTEDTLVSATKYRDHVLGCLEEDL--AGAPRAEWCGGHNRQLRRQLAESLIKAMGACKRCENCGAFSPNIRKDGSNKLFQVPLSDKNRMANQS--CNTQIVSAVQRRDPGKPNEDXXXXXXXXXXXXXXXXXXXXXXXDDVGGSGPSSKVVGVPLAAAQAAAGDPAAKTGAQAHKFMPPIEVELQMQQLWKQESSTLDLIFVSGRRTRGVPGDVNGAPSSSSQNADGLSGGGIADGYRLFFVRALAVPPPRFRPPMNMGD-MIAEHPQNVYLTKVLNLNESLK-------------NADSLKGEDLASVLTTWVELQTTVNCYMDSSKDPR---GLKDTPPGLRQVLEKKEGLFRKHMMGKRVNYACRSVISPDPYLGTTEIGIPLRFAKELTYPQPVADWNVEAMRQLVENGTSVYPGANFVEDSSGRMLHLDRLSDLKRRGVAARLLSSPGQKVWRHLTDGDCMLVNRQPTLHKPGIMAHRVRVLRNPGYQTIRMHYANCNTYNADFDGDEINCHLPQNELAKAEANLLAFTDEQYLVPTNGKPLRGLIQDHVDAGVKMCSKDCFFTRGEYQQLLYQALSGLPGLEIVPPSDDIATLPPAILKPQQRWTGKQVMSTILRHLTKGLPQLNLDGKSRTPKVAFGEAEQEHMIIFREGELLQGVLDKGAFGSSEFGLVHAVHEVYGSTAAGKLLTALGRVLTIFLQSSGHTCGIEDLTLTARAEGNRREIINKSLGIGQRSMRELVDN-AGEGQNAKAIANGSATEKGDEDTGEALVEAEVEGIRQKTASFLLGEDRDSRL---AEIDRHMQSALAPVSSDIIKACLPGGQAKPFPHNCFSLMVLTGAKGSMVNHSQVSCALGQQALEGRRVPVMVSGKSLPSFQAFEPSPRANGFITDRFLTGIRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEELKVGYDNTVRDGEGCVHQFLYGEDGVDTTQTKYLSSK--QLGFLAQNYQALRHKHGITPSFKKDAGFDSREASRAHSEIATA------QRRRDDAAGAASASGAKFVAGEMVLAKRRAVLKGGEGWGKAELLAGWHSAEVVKARNA--GTSAALYSVRYKDDGAMGKKMP-----VSAAQEGPSESFADPSPEENNESGHLSSRRRKRPRRLLRKCPGAGLPDPVL--STLSVNKDLGCVSEAFQKSLQAFLDSNPASLRGDHTKENLKAVAATDTSSPPVLGSLKEAFELLMWVKYMRCLANPGETVGSIAAQSVGEPSTQMTLNTFHLAGHGGANVTLGIPRLREIIMTAAKNLKTPSMVVPLREGADRPAAKSLALRLSRLPLSQLLDN-RGGIVVRERLVK-GDTGL-WERHYAISLKLFPPSVINRAFDISFKELCRKISGVFVPNLLSAIANDLRRTGNKLSQTGGASVGLPSKGRGKRAD------GDRVEEEDDLEGERSAIAQS-KAVAKKASKAEXXXXXXXXXXE--------------QGTLKFGKQKEQSSYGGMDEEEKAIWKAMSGKEAGGGVLGDDDHXXXXXEGREGVDGVG---------KDKDDDRDYFNLPNLSGARH--FKGITKNKKTGEVEVTVRLPSSMRRLLMVGLAESAAAKTMVRSHKGIRGAFVVETTVDGKSG-LAVQLEGSDFETVWQLKEGGVDGILNLSKLTSNDIWAVCQTYGVEAARASIVAEITGVFGAYGIAVDPRHLGLVADHMTFDGGYRPLNRAGMADFSSPCLQMSFETTTDFMAKAAVARSSDRLKSPSARIVMGRVGNFGTGMFDLRQPAE 5776
            +I   +R V+RH V E+ FG   +++I RLSV R+++  T+D L NPLPGGLYDP +GP+D   +C TC    ++CPGHMGH  L++ VY+P+ FG +++ LR KCF CH  RLS     V A+K+ L+DAG A EA  LD  L  +  D  R           T++  T+ R      L   L  AG     W  G  +  RR L +  ++ + +CK C+ C A    +RKDG  K F      + R A  S      I SA+     G+   +                       DD  G   S         A+Q         T A+  ++M P EV++ M+ LW+ E     LI+  G+    V   V  AP + +         G ADG++  F R L V P RFRPP    D  +AEHPQN  + K++NL+E ++               ++L  E L+ VL+TW++LQ  VN Y+DSSKD     G  +  PG+RQ LEKKEGLFR HMMGKRVN+ACRSVISPDPY+GT+EIG+PLRFAK LTY QPV  +N   +R+ VE G   +PGAN+VED+ GR++ L R+   +RR +AARLL+ PGQ VWRHL DGD +L+NRQPTLHKPGIMAHR RVLR+P  QTIRMHYANCNTYNADFDGDE+NCH PQ+EL++AEA  +A TDEQYL PTNGKPLRGLIQDHV +GVK+  KDCF +R ++Q +L+  LSGLPG+E+VPP   I    PAI KPQ+ WTGKQ++S +L+HL++GLP LNLD K++TP VAFG  ++EH ++ R G+L++GVLDK AFG+SE GLVH V+E YG   AG+LLTALGR+ T++LQ +GHTCG+EDL L+  AE  RR +I ++  +G  +M       A E      + + S   + D  + + L  A++  ++ +TA  L  + R  ++   A ID  M++ L+PVSSDIIKACLP G  KPFPHN FSLMV TGAKGS VN SQ+SC LGQQ LEGRRVP+M SGKSLPSF  ++PSPRA GFI DRFLTG+RPQEYYFHCMAGREGL+DTAVKTSRSGYLQRCLVK LEEL+V YDNTVRD +  V QFLYGEDGVD    KYL  +  Q+  LA+N+ AL  ++ +   F +  G D + A   H EI  A      Q ++ +    ++   A+   G MVL KR  + +    W     L GW  AEVVK   A   +  A Y +RY  DG + K++P            P+ S  D  P                   L+R      LPDPVL  +   +   LG +SE FQ +++ +L  +P  L         K+               K   ELL+W+KYMR L +PGE VGSIA QS+GEPSTQMTLNTFHLAGHGGANVTLGIPRLREIIMTA++N KTPSM VPL     R  A+ L+ RL RL L +++ + +GG++V+ERL +   +G+ W+R+Y +SL+L    V+  AF +S +E+   I+  FV  LL+++  +LR+  ++        V L  + RG  AD      G+  E+E D+ G         KA A+  +K            E              QGTL FG+++E+  Y    E ++              +              EGV+  G         K + +   + ++  ++   H  F+ +   ++TG   +T++  +S RRLLMV + E+AA +  VR  KGI  AFVVE    GK G  ++Q EG +   +W++     D  L+L+ +TSNDIWA+ QTYGVEAARA+IV EI GVFGAYGIAVDPRHLGLVAD MT+ GG+R LNR GM++ SSP LQMSFETT  F+ +AA+    D L++PSA++VMG+    GTG FD+  P +
Sbjct:    6 SITMTERCVVRHDVSEVQFGVYKEEEILRLSVVRVSTAATYDRLGNPLPGGLYDPAMGPLDARSICVTCNLSMRDCPGHMGHFPLSLHVYNPLTFGIVFQALRVKCFGCHGFRLSTFPCNVLALKLGLIDAGLAHEADSLDQAL--FRADLHRS----------TILRDTEMR------LRRHLQTAGQVPKSWIPGETKIKRRALIKDFLQEVLSCKTCDRCSAPKTTVRKDGYTKFFV-----RGRRAGHSRGAGAHIPSALAVVRNGRHEVECGNGFG-----------------DDSDGE-ESEXXXXXXXXASQERFAARGGDTEAEGGEYMAPAEVQMHMKLLWEAEPELATLIW--GKAA--VEKSVFSAPGADT---------GRADGWKTLFWRVLPVTPSRFRPPSKNDDGSLAEHPQNYNVIKIMNLDERIRALLRKEDGGEGATGPENLPAEALSRVLSTWIDLQNAVNGYIDSSKDANARLGGGNLTPGIRQGLEKKEGLFRMHMMGKRVNFACRSVISPDPYIGTSEIGLPLRFAKVLTYSQPVTPFNAAQLREAVERGAEGHPGANYVEDARGRLIDLRRMDAGRRRALAARLLTPPGQTVWRHLQDGDMVLMNRQPTLHKPGIMAHRARVLRSPAQQTIRMHYANCNTYNADFDGDEMNCHFPQDELSRAEAETIACTDEQYLAPTNGKPLRGLIQDHVGSGVKLTGKDCFLSRADFQHILFCTLSGLPGVEVVPPYGHIYMPAPAIWKPQELWTGKQMISVLLQHLSEGLPPLNLDFKAKTPAVAFGVDQEEHEVVVRGGDLIRGVLDKAAFGASEGGLVHGVYEFYGPRFAGRLLTALGRLFTVYLQFAGHTCGVEDLVLSRAAEQRRRALITRAGRLGGLAMYCYTSGTAFEPPPLPPLPSASGQAEKDGASADGLSAADLARVQARTAELLEEDMRTGQVTQAAAIDNFMRTVLSPVSSDIIKACLPDGLTKPFPHNAFSLMVSTGAKGSTVNQSQISCGLGQQELEGRRVPLMPSGKSLPSFPPYDPSPRAGGFIVDRFLTGVRPQEYYFHCMAGREGLIDTAVKTSRSGYLQRCLVKGLEELRVHYDNTVRDADAGVVQFLYGEDGVDPLHGKYLDGQAPQMTALARNFAALLCQYKVDLKFLERTGMDLKTARAMHEEIKAARLQIKEQAQKREQKSLSAVRPAQLKVGNMVLTKR--LKRTRTEWITGAWLPGWEPAEVVKVHGAKGASRPATYDLRYISDGKVAKRVPRRIRHTVVEPSRPAASALDIPPXXXXXXASQVD--------LIR---WPYLPDPVLGRAKTQLGLHLGAISERFQDAIEDYLGRDPEHLFQKSEMGGSKS---------------KAGLELLLWLKYMRSLTSPGEAVGSIAGQSIGEPSTQMTLNTFHLAGHGGANVTLGIPRLREIIMTASRNPKTPSMTVPLCTSISRSEAQRLSARLRRLQLLEVVHHTQGGVLVKERLGRCASSGVTWQRYYTVSLRLHDEEVLQEAFGLSHEEMFEVIATRFVRQLLASVTAELRKVASR------DVVRLLKERRGNAADAEARESGEASEDEADMTGLVGGRGPGMKAAARAGAKTSPKLAAMDVDGEEXXXXXXXXXGDEAQGTLSFGRKEERGEY----EPDEXXXXXXXXXXXXXXI-------------EEGVENSGEGQLSEVPAKQRRNGSRFDDVAAVAKIDHPFFENLRFERETGVAHITLKTRASHRRLLMVSIVEAAAERCTVRVSKGIAQAFVVE----GKDGRCSIQTEGCNLSELWEVG----DAALDLNNITSNDIWAMHQTYGVEAARATIVKEIKGVFGAYGIAVDPRHLGLVADFMTYQGGFRALNRIGMSEVSSPFLQMSFETTATFLTEAALNSEKDDLQTPSAKLVMGQPTGNGTGSFDIMIPVD 1848          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16194.1 vs. uniprot
Match: A0A024TY59_9STRA (DNA-directed RNA polymerase subunit n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024TY59_9STRA)

HSP 1 Score: 1410 bits (3651), Expect = 0.000e+0
Identity = 863/1921 (44.92%), Postives = 1145/1921 (59.60%), Query Frame = 2

Query:  158 DRTVIRHAVEEIGFGFLTDDDIRRLSVKRLTSPVTFDTLNNPLPGGLYDPVLGPVDKMMMCETCGQDQKNCPGHMGHIELAVSVYHPILFGALYKLLRAKCFSCHNLRLSKSKTRVAAVKMMLVDAGRAQEALELDATLLGYLKDGARDHDVLEGNTEDTLVSATKYRDHVLGCLEE-DLAGAPRAEWCGGHNRQLRRQLAESLIKAM--GACKRCENCGAFSPNIRKDGSNKLFQVPLSDKNRMANQSCNTQIVSA---VQRRDPGKPNEDXXXXXXXXXXXXXXXXXXXXXXXDDVGGSGPSSKVVGVPLAAAQAAAGDPAAKTGAQAHKFMPPIEVELQMQQLWKQESSTLDLIFVSGRRTRGVPGDVNGAPSSSSQNADGLSGGGIADGYRLFFVRALAVPPPRFRPPMNMGDMIAEHPQNVYLTKVLNLNESLKN-------------ADSLKGEDLASVLTTWVELQTTVNCYMDSSKDPRGLKDTPPGLRQVLEKKEGLFRKHMMGKRVNYACRSVISPDPYLGTTEIGIPLRFAKELTYPQPVADWNVEAMRQLVENGTSVYPGANFVEDSSGRMLHLDRLSDLKRRGVAARLLSSPG-----------QKVWRHLTDGDCMLVNRQPTLHKPGIMAHRVRVLRNPGYQTIRMHYANCNTYNADFDGDEINCHLPQNELAKAEANLLAFTDEQYLVPTNGKPLRGLIQDHVDAGVKMCSKDCFFTRGEYQQLLYQALSGLPGLEIVPPSDDIATLPPAILKPQQRWTGKQVMSTILRHLTKGLPQLNLDGKSRTPKVAFGEAEQEHMIIFREGELLQGVLDKGAFGSSEFGLVHAVHEVYGSTAAGKLLTALGRVLTIFLQSSGHTCGIEDLTLTARAEGNRREIINKSLGIGQRSMRELVDNAGEGQNAKAIANGSATEKGDEDTGEALVEAEVEGIRQKTASFLLGEDRDSRLAEIDRHMQSALAPVSSDIIKACLPGGQAKPFPHNCFSLMVLTGAKGSMVNHSQVSCALGQQALEGRRVPVMVSGKSLPSFQAFEPSPRANGFITDRFLTGIRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEELKVGYDNTVRDGEGCVHQFLYGEDGVDTTQTKYLSSK--QLGFLAQNYQALRHKHGITPSFKKDAGFDSREASRAHSEIATAQRRRDDAAGAASASGAKFVAGEMVLAKRRAVLKGGEG-WGKAELLAGWHSAEVVKARNAGTSAALYSVRYKDDGAMGKKMPVSAAQEGPSESFADPSPEENNESGHLSSRRRKRPRRLLRKCPGAGLPDPVLSTLSVNKDLGCVSEAFQKSLQAFLDSNPASLRGDHTKENLKAVAATDTSSPPVLGSLKEAFELLMWVKYMRCLANPGETVGSIAAQSVGEPSTQMTLNTFHLAGHGGANVTLGIPRLREIIMTAAKNLKTPSMVVPLREGADRPAAKSLALRLSRLPLSQLLDNRGGIVVRERLVKG-DTGLWERHYAISLKLFPPSVINRAFDISFKELCRKISGVFVPNLLSAIANDLRRTGNKLSQTGGASVGLPSKGRGKRADGDRVEEEDDLEGERSAIAQSKAVAKKASKAEXXXXXXXXXXEQGTLKFGKQKEQSSYGGMDEEEKAIWKAMSGKEAGGGVLGDDDHXXXXXEGREGVDGVGKDKDDDRDYFNLPNLSG-------------ARHFKGITKNKKTGEVEVTVRLPSSMRRLLMVGLAESAAAKTMVRSHKGIRGAFVVETTVDGKSGL---AVQLEGSDFETVWQLKEGGVDGILNLSKLTSNDIWAVCQTYGVEAARASIVAEITGVFGAYGIAVDPRHLGLVADHMTFDGGYRPLNRAGMADFSSPCLQMSFETTTDFMAKAAVARSSDRLKSPSARIVMGRVGNFGTGMFDLRQP 5770
            D++++RH V E+ FGF +DD+IR LSVK++TS ++FDTL NP+ GGLYDP LGP+D  M+C TC   QK CPGH+GHIEL V VY P+LF  L  +L+ KC SCH  R   + +RV  V+++L+D G  +EA    A+LL  L       D  +GN +++     + +  +L   E   L+G  +A      + ++ R   +++I A   G   +CENC A SP+IR+D   K+F  PLS ++   NQS   ++ SA   + +   G   +D XXXXXXXXXXXXXXXX       +  G                             A++++ P+EV  Q+Q LW+ E   ++L++ +     G   D N +                 DG+R FF++ + V P RFRPP+ MGD   EH QN+YL+K++ L+E L N             ADS  G DL+  +  W ELQT VNC +D+SK  R   D P G++Q++EKKEGLFRKHMMGKRVNYA RSVISPDPY+ T+EIG+PLRFAK LT PQ V  WNV  MRQLVENG  V+PGANFVE+  G+++ L + +  +R  +   LL+              ++VWRHL  GD +L+NRQPTLHKP +MAH  RVL NP  QTIRMHYANCNTYNADFDGDE+N H PQNELA+AEA  +A  D QY+VPT+G PLRGLIQDHVD+GVK+  +D F T+  Y QL+Y A + L          +I  LPP I+KP   WTGKQVMSTIL HLT G P+LNL+ K++     +G    EH+++FR+GELLQGVLDKG FG++E+G VHAV+E+YGS  A KLLTALGRVLT +LQ +GHTC +EDLTLT  AE  RR ++  ++  G+ +  E    A      KA A+ +       D+   + + E   IR+ T    + ED +   A +D HM   +   SSDIIKACLP GQ K FP NCFSLMVLTGAKGSMVNHSQ+SC LGQQALEGRRVPV+VSGKSLPSF+A++P+PRA G+ITDRFLTG+RPQEYY HCMAGREGLVDTAVKTSRSGYLQRCL+KHLE+L V YD+TVR+ EG V QFLYGEDGVD  Q  +LS K  QL FLA N+ +L HK GI+  F +++G D  E +  H  I  A+R   D A  A+ +G +    ++ LA +   LK G   W K     GWH A +V+  +AG    +Y ++Y D G     +P     +  S++    SP+ +  SG +          LLR      LPDPV+  L +N  +G VSE  Q  ++A+   NPA L      + L A A     SP +L  L   F L++WVKYMR L  PGE VG+I AQS+GEPSTQMTLNTFHLAGHG ANVTLGIPRLREIIMTA++ + TP M VPL       AA+++  RL+++PL++LL N  GI V++   +  +  LW R Y I L  F    I  AF IS KE+ R    VFV  LL+ + +++R++G  LS +      + +    +    D   E+DD                                 QGT++FG +KE S YG MD++++AI      +        DD       +G +G D      DD+ D   L  ++G             + +F G   N K    E+ +R P++ + LL+V L E  A+  +VRS  GI   + V+  + G+S +    +Q  G +F+ +W+L     D +L++++L +NDI+ V + YGVEAARASI  +I  VFG YGI+VDPRHL L+AD+MT  G Y PLNR+GM    S   Q++FET+  F+  AA A   D +  PSAR+V+G+    GTG F L  P
Sbjct:    6 DQSILRHEVSEVCFGFYSDDEIRDLSVKQITSRISFDTLKNPVLGGLYDPALGPIDFNMICPTCHLTQKECPGHLGHIELPVPVYSPVLFTTLLNILKRKCMSCHKFRRHSANSRVYRVRILLLDNGYVEEA----ASLLPLL-------DQKDGNFDESANQTLQRQQAILDEFERLALSGTKKALPRNARSVEVER---DAIINAFLKGMTNKCENCEAHSPSIRQDAHAKIFLKPLSLRSIKHNQSRAVRLSSAFDVLSKYSTGTNKDDSXXXXXXXXXXXXXXXXTKNAASAENQG-----------------------------ANQYLAPLEVMSQLQLLWQHEEGLMELMWGNRLVANGRSPDANAS----------------MDGWRKFFLQVIPVAPSRFRPPVIMGDSQFEHSQNIYLSKIITLSEQLVNVSGMRRGHASSTAADSDTGVDLSEKIYLWTELQTQVNCLIDNSKAKRP-DDVPQGIKQLIEKKEGLFRKHMMGKRVNYAARSVISPDPYISTSEIGVPLRFAKTLTLPQAVTPWNVVEMRQLVENGPDVHPGANFVENERGQLIDLSKRTHHQRVAIGKTLLTRSASAHGAHQEHKVKRVWRHLHSGDVVLMNRQPTLHKPSMMAHVTRVLTNPAMQTIRMHYANCNTYNADFDGDEMNMHFPQNELARAEAYTIANNDNQYIVPTDGSPLRGLIQDHVDSGVKLTQRDTFLTKDLYIQLVYSAWACLDHER----GGEIDILPPTIVKPTPLWTGKQVMSTILHHLTHGKPKLNLNAKAKIKADLYGPQNAEHVVVFRDGELLQGVLDKGQFGATEYGWVHAVYELYGSGTAAKLLTALGRVLTCYLQYAGHTCALEDLTLTEEAEAERRRLVQHAVVHGEIAYSEFAGLADVAAARKAEADAN------HDSVRLMNDVERTKIRE-TMQKRMAEDLEQTSAGLDSHMMGFVHGSSSDIIKACLPHGQNKSFPANCFSLMVLTGAKGSMVNHSQISCGLGQQALEGRRVPVLVSGKSLPSFEAYDPNPRAGGYITDRFLTGLRPQEYYHHCMAGREGLVDTAVKTSRSGYLQRCLIKHLEDLHVAYDHTVRNAEGTVVQFLYGEDGVDPMQAAFLSGKADQLSFLAMNHASLTHKFGISGEFLENSGLDVVEPAHVHQRIKDAKRYGYDRA--ATTAGLEMHTMKVGLAIQARRLKAGASKWRKGNFELGWHDAVIVQVDDAGDYP-VYDIQYMDTGLTAHAVPEYKRFKA-SDTI---SPDVSYLSGTVQ---------LLRPV----LPDPVMHQLPLNAHIGVVSEKTQLDIEAYAKGNPAGLLESKKTKALPAAA----KSPMMLSPL--GFRLMVWVKYMRSLCQPGENVGTICAQSIGEPSTQMTLNTFHLAGHGAANVTLGIPRLREIIMTASQKMSTPMMTVPLLPTTSAAAAQAVEQRLNQVPLAELLRNTKGIRVQDEFKESANRVLWVREYTIRLSFFKAKAIRSAFGISMKEVHRSFGRVFVSRLLTLLKHEIRKSGVALSTSRAVDNDIETTATTRFRKHDDDNEDDD-------------------------------EAQGTMRFGAKKEASGYGEMDDDDRAILAQQLNE--------DDAPRAGATDGSDGSD-TENSSDDEVDAAPLKTVAGEIDPVPVTETVRKSPYFAGAGFNSKEQFGELKLRFPANFKTLLLVPLIEKVASHVLVRSCPGISRCYQVKQRL-GRSDVEEQCIQTAGLNFQAIWEL-----DDVLDVNRLATNDIYQVLRHYGVEAARASISKQIQDVFGVYGISVDPRHLNLLADYMTHHGDYMPLNRSGMVHKGSSFQQITFETSMKFLTAAATANLGDSMDGPSARLVLGQPVKLGTGSFSLLTP 1783          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16194.1 vs. uniprot
Match: A0A1V9ZEK2_9STRA (DNA-directed RNA polymerase subunit n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZEK2_9STRA)

HSP 1 Score: 1400 bits (3624), Expect = 0.000e+0
Identity = 851/1917 (44.39%), Postives = 1139/1917 (59.42%), Query Frame = 2

Query:  152 SHDRTVIRHAVEEIGFGFLTDDDIRRLSVKRLTSPVTFDTLNNPLPGGLYDPVLGPVDKMMMCETCGQDQKNCPGHMGHIELAVSVYHPILFGALYKLLRAKCFSCHNLRLSKSKTRVAAVKMMLVDAGRAQEALELDATLLGYLKDGARDHDVLEGNTEDTLVSATKYRDHVLGCLEEDLAGAPRAEWCGGHNRQL---RRQLAESLIKAMGACKRCENCGAFSPNIRKDGSNKLFQVPLSDKNRMANQSCNTQIVSAVQ--RRDPGKPNEDXXXXXXXXXXXXXXXXXXXXXXXDDVGGSGPSSKVVGVPLAAAQAAAGDPAAKTGAQAHKFMPPIEVELQMQQLWKQESSTLDLIFVSGRRTRGVPGDVNGAPSSSSQNADGLSGGGIADGYRLFFVRALAVPPPRFRPPMNMGDMIAEHPQNVYLTKVLNLNE---SLKNADSLKGED------LASVLTTWVELQTTVNCYMDSSKDPRGLKDTPPGLRQVLEKKEGLFRKHMMGKRVNYACRSVISPDPYLGTTEIGIPLRFAKELTYPQPVADWNVEAMRQLVENGTSVYPGANFVEDSSGRMLHLDRLSDLKRRGVAARLL----SSPG-------QKVWRHLTDGDCMLVNRQPTLHKPGIMAHRVRVLRNPGYQTIRMHYANCNTYNADFDGDEINCHLPQNELAKAEANLLAFTDEQYLVPTNGKPLRGLIQDHVDAGVKMCSKDCFFTRGEYQQLLYQALSGLPGLEIVPPSDDIATLPPAILKPQQRWTGKQVMSTILRHLTKGLPQLNLDGKSRTPKVAFGEAEQEHMIIFREGELLQGVLDKGAFGSSEFGLVHAVHEVYGSTAAGKLLTALGRVLTIFLQSSGHTCGIEDLTLTARAEGNRREIINKSLGIGQRSMRELVDNAGEGQNAKAIANGSATEKGDEDTGEALVEAEVEGIRQKTASFLLGEDRDSRLAEIDRHMQSALAPVSSDIIKACLPGGQAKPFPHNCFSLMVLTGAKGSMVNHSQVSCALGQQALEGRRVPVMVSGKSLPSFQAFEPSPRANGFITDRFLTGIRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEELKVGYDNTVRDGEGCVHQFLYGEDGVDTTQTKYLSSK--QLGFLAQNYQALRHKHGITPSFKKDAGFDSREASRAHSEIATAQRRRDDAAGAASASGAKFVAGEMVLAKRRAVLKGGEGWGKAELLAGWHSAEVVKARNAGTSAALYSVRYKDDGAMGKKMPVSAAQEGPSESFADPSPEENNESGHLSSRRRKRPRRLLRKCPGAGLPDPVLSTLSVNKDLGCVSEAFQKSLQAFLDSNPASLRGDHTKENLKAVAATDTSSPPVLGSLKEAFELLMWVKYMRCLANPGETVGSIAAQSVGEPSTQMTLNTFHLAGHGGANVTLGIPRLREIIMTAAKNLKTPSMVVPLREGADRPAAKSLALRLSRLPLSQLLDNRGGIVVRERLVKGDTG-LWERHYAISLKLFPPSVINRAFDISFKELCRKISGVFVPNLLSAIANDLRRTGNKLSQTGGASVGLPSKGRGKRADGDRVEEEDDLEGERSAIAQSKAVAKKASKAEXXXXXXXXXXEQGTLKFGKQKEQSSYGGMDEEEKAIWKAMSGKEAGGGVLGDDDHXXXXXEGREGVDGVG-KDKDDDRDYFNLPNLSGAR-------------HFKGITKNKKTGEVEVTVRLPSSMRRLLMVGLAESAAAKTMVRSHKGIRGAFVVETTVD--GKSGLAVQLEGSDFETVWQLKEGGVDGILNLSKLTSNDIWAVCQTYGVEAARASIVAEITGVFGAYGIAVDPRHLGLVADHMTFDGGYRPLNRAGMADFSSPCLQMSFETTTDFMAKAAVARSSDRLKSPSARIVMGRVGNFGTGMFDLRQP 5770
            S D+T++RH V ++ FGF +DD+IR LSVK+++S V+FD+L NP+ GGLYDP LGP+D  M+C TC   QK CPGH+GHIEL V VY P+LF  +  +L+ KC SCH  R S S TR+  V+++L+D G   EA ++   L           D  +GN E+        +  +L   E  LA +  A +   + R +   R  +  S +K M    +CENC A SP +R+D   K+F  PLS +++  NQS    + SA +    +  KP  D                       +D+                   AA D A  +    H ++ P+EV  QMQ LW+ E    +L++  G RT         A S   +N          DG++ FF+  + V P RFRPP+ MGD   EH QN+YL+K+++L E   ++ NA  L  E+      L+  +  W ELQT VNC +D+SK  R  +D P G++Q++EKKEGLFRKHMMGKRVNYA RSVISPDPY+ T+EIG+PLRFAK LT PQ V  WNV  MRQLVENG  ++PGANFVE+  G+++ L + +  +RR +   LL    S+ G       ++VWRHL  GD +L+NRQPTLHKP +MAH  RVL NP  QTIRMHYANCNT+NADFDGDE+N H PQNELA++EA  +A  D QYLVPT+G PLRGLIQDHVD+GVK+  +D F T+  Y QL+Y A + L G + V     I TLPP ILKPQ  WTGKQVMSTI+  LT G P LNL+ K++     +G    EH+IIFR+GELLQGVLDK  FG+SE+G VHA +E+YGS  A KLLTALGRVLT +LQ SGHTC +EDLTL   AE  RR ++ +S+  G+ +  E    +G G   + + +                  E     + T    + +D ++  A +D HM   +   +S+IIK+CLP GQ K FP NCFSLMVLTGAKGSMVNHSQ+SC LGQQALEGRRVPV+VSGKSLPSF+ F+PSPRA G+ITDRFLTG+RPQEYY HCMAGREGLVDTAVKTSRSGYLQRCL+KHLE+L V YD+TVR+ +G V QFLYGEDGVDT  + +LS K  Q+ FLA N+ +L HK GIT  F + +G D  E ++ H +I  A+RR     G  +         E ++   R +  G   W K     GWH A V+K  + G    +Y + YKD G    K+P     +  + +     PE    +G++          L++      LPDPV+  L +N  +GCVSE  Q+ ++ +  +NP++L  +  K  +  V ++             AF L++WVKYMR L  PGE VG+I AQS+GEPSTQMTLNTFHLAGHG ANVTLGIPRLREIIMTA+K + TP M +P+   A    A ++  +L+++PL++L+ N  GI V+++ ++ +   LW R Y I L  F P +I  AF I+ KE+ R     FV  LL+ + +++R++G ++S   G S+ + SK        D +EEED         A+    AK        XXXXXXXX+QGTLKFG QKE + YG MD+++  I KA +          DD       E  +  DG   ++  DD D   +  + G               +F G   N      E+ +R P++ + LL+V L E  A++ +VRS  GI   + V+  +D  GK    +Q +G +FE +W+L     D +L++++L +NDI+ V   YGVEAARASI  +I  VFG YGI+VDPRHL L+AD+MT  G Y PLNR+GMA   S   Q++FET+  F+ +AA     D +  PSAR+V+G+    GTG+F L  P
Sbjct:    4 SMDQTILRHEVSDVCFGFYSDDEIRALSVKQISSRVSFDSLKNPVIGGLYDPALGPIDFNMICPTCHMTQKECPGHLGHIELPVPVYSPVLFATMLNILKRKCMSCHKFRKSASTTRIYRVRLLLLDNGYIDEAAQMLELL-----------DQKDGNFEEGYEQTVMRQQAILDEYER-LALSGTATYLPKNPRSVEVERDNIINSFLKGMTY--KCENCEAHSPGVRQDSHAKIFLKPLSLRSQRHNQSRAVHLNSAFEMININDKKPAADDSDSEAD----------------EDMD------------------AAKDEANTSN---HTYLAPLEVMSQMQLLWQNEEGVTELMW--GNRT---------AASRRGKNH-------ALDGWKKFFLNVIPVAPSRFRPPVIMGDSQFEHSQNIYLSKIISLAEQMVTMGNARRLAEEEAPQSINLSDKIYLWTELQTQVNCLIDNSKAKRP-EDMPQGIKQLIEKKEGLFRKHMMGKRVNYAARSVISPDPYISTSEIGVPLRFAKTLTLPQAVTPWNVIEMRQLVENGPDIHPGANFVENERGQLIDLSKRTLHQRRAIGKTLLTRSASAQGAHQKHKVKRVWRHLKSGDVVLMNRQPTLHKPSMMAHVTRVLTNPAMQTIRMHYANCNTFNADFDGDEMNMHFPQNELARSEAYNIANNDNQYLVPTDGSPLRGLIQDHVDSGVKLTQRDTFLTKDMYLQLVYSAWTCLDGEKGV-----IKTLPPTILKPQPLWTGKQVMSTIVLMLTAGKPSLNLNSKAKIKADLYGPGNAEHIIIFRDGELLQGVLDKAQFGASEYGWVHACYELYGSGTAAKLLTALGRVLTCYLQYSGHTCALEDLTLNEHAEKERRRLVEQSVVQGEIAYSEFAGLSGLGDKERRMND------------------EERAKIRDTMQLKMAQDLENTSAALDSHMMGFVHGSNSEIIKSCLPHGQNKSFPANCFSLMVLTGAKGSMVNHSQISCGLGQQALEGRRVPVLVSGKSLPSFEPFDPSPRAGGYITDRFLTGLRPQEYYHHCMAGREGLVDTAVKTSRSGYLQRCLIKHLEDLHVAYDHTVRNCDGNVVQFLYGEDGVDTINSAFLSGKPDQMSFLAMNHASLTHKFGITAEFLEKSGMDVVEPAKIHHQIKDAKRR-----GYENGDFELHTMKEGLVVLARRLKDGHHKWKKGHFELGWHEA-VIKNVDDGGEFPVYDLTYKDTGLTVYKVPEFKFFKASTSTM----PENAYMAGNVQ---------LIK----PQLPDPVMHALQLNNHIGCVSEKTQQHIETYSKANPSNLL-ESKKNKIGGVVSSS------------AFRLMVWVKYMRSLCQPGENVGTICAQSIGEPSTQMTLNTFHLAGHGAANVTLGIPRLREIIMTASKKMSTPMMTIPVLPTATPAQAVAVEQKLNQVPLAELIRNTKGIRVQDQFLESENRVLWCREYTIRLYFFKPKLIREAFGITTKEIQRAFGKSFVSRLLTLLKHEMRKSGVQVSVENG-SMSVRSK--------DNIEEED---------AEPTRRAKXXX-----XXXXXXXXDQGTLKFGSQKEAAGYGEMDDDDVEIQKAQNT---------DDQDMDESAEKSKQADGSDTENSSDDEDVAPIKVIKGELKPVNVTDTVNKNPYFAGAGVNTTEHYGELKLRFPANFKTLLLVPLIEKIASQVLVRSCDGISRCYTVKQRLDKSGKEEQCIQTQGLNFEAIWEL-----DHVLDVNRLVTNDIYQVLLNYGVEAARASISKQIQDVFGVYGISVDPRHLSLLADYMTHYGDYMPLNRSGMARKGSSFQQITFETSMKFLTQAATGNFGDNMSGPSARLVLGQPVKLGTGLFSLMTP 1754          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16194.1 vs. uniprot
Match: W4FVL2_9STRA (DNA-directed RNA polymerase subunit n=12 Tax=Aphanomyces TaxID=100860 RepID=W4FVL2_9STRA)

HSP 1 Score: 1389 bits (3596), Expect = 0.000e+0
Identity = 856/1932 (44.31%), Postives = 1130/1932 (58.49%), Query Frame = 2

Query:  158 DRTVIRHAVEEIGFGFLTDDDIRRLSVKRLTSPVTFDTLNNPLPGGLYDPVLGPVDKMMMCETCGQDQKNCPGHMGHIELAVSVYHPILFGALYKLLRAKCFSCHNLRLSKSKTRVAAVKMMLVDAGRAQEALELDATLLGYLKDGARDHDVLEGNTEDTLVSATKYRDHVLGCLEE-DLAGAPRAEWCGGHNRQLRRQ-LAESLIKAMGACKRCENCGAFSPNIRKDGSNKLFQVPLSDKNRMANQSCNTQIVSAVQRRDPGKPNEDXXXXXXXXXXXXXXXXXXXXXXXDDVGGSGPSSKVVGVPLAAAQAAAGDPAAKTGAQAHKFMPPIEVELQMQQLWKQESSTLDLIFVSGRRTRGVPGDVNG-APSSSSQNADGLSGGGIADGYRLFFVRALAVPPPRFRPPMNMGDMIAEHPQNVYLTKVLNLNESLKN--------------ADSLKGEDLASVLTTWVELQTTVNCYMDSSKDPRGLKDTPPGLRQVLEKKEGLFRKHMMGKRVNYACRSVISPDPYLGTTEIGIPLRFAKELTYPQPVADWNVEAMRQLVENGTSVYPGANFVEDSSGRMLHLDRLSDLKRRGVAARLLSSPG-----------QKVWRHLTDGDCMLVNRQPTLHKPGIMAHRVRVLRNPGYQTIRMHYANCNTYNADFDGDEINCHLPQNELAKAEANLLAFTDEQYLVPTNGKPLRGLIQDHVDAGVKMCSKDCFFTRGEYQQLLYQALSGLP---GLEIVPPSDDIATLPPAILKPQQRWTGKQVMSTILRHLTKGLPQLNLDGKSRTPKVAFGEAEQEHMIIFREGELLQGVLDKGAFGSSEFGLVHAVHEVYGSTAAGKLLTALGRVLTIFLQSSGHTCGIEDLTLTARAEGNRREIINKSLGIGQRSMREL--VDNAGEGQNAKAIANGSATEKGDEDTGEALVEAEVEGIRQKTASFLLGEDRDSRLAEIDRHMQSALAPVSSDIIKACLPGGQAKPFPHNCFSLMVLTGAKGSMVNHSQVSCALGQQALEGRRVPVMVSGKSLPSFQAFEPSPRANGFITDRFLTGIRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEELKVGYDNTVRDGEGCVHQFLYGEDGVDTTQTKYLSSK--QLGFLAQNYQALRHKHGITPSFKKDAGFDSREASRAHSEIATAQRRRDDAAGAASASGAKFVAGEMVLAKRRAVLKGGEGWGKAELLAGWHSAEVVKARNAGTSAALYSVRYKDDGAMGKKMPVSAAQEGPSESFADPSPEENNESGHLSSRRRKRPRRLLRKCPGAGLPDPVLSTLSVNKDLGCVSEAFQKSLQAFLDSNPASLRGDHTKENLKAVAATDTSSPPVLGSLKEAFELLMWVKYMRCLANPGETVGSIAAQSVGEPSTQMTLNTFHLAGHGGANVTLGIPRLREIIMTAAKNLKTPSMVVPLREGADRPAAKSLALRLSRLPLSQLLDNRGGIVVRERLVKGDTG-LWERHYAISLKLFPPSVINRAFDISFKELCRKISGVFVPNLLSAIANDLRRTG----------NKLSQTGGASVGLPSKGRGKRADGDRVEEEDDLEGERSAIAQSKAVAKKASKAEXXXXXXXXXXEQGTLKFGKQKEQSSYGGMDEEEKAIWKAMSGKEAGGGVLGDDDHXXXXXEGREGVDGVGKDKDDDRDYFNLPNLSG-------------ARHFKGITKNKKTGEVEVTVRLPSSMRRLLMVGLAESAAAKTMVRSHKGIRGAFVVETTVDGKS--GLAVQLEGSDFETVWQLKEGGVDGILNLSKLTSNDIWAVCQTYGVEAARASIVAEITGVFGAYGIAVDPRHLGLVADHMTFDGGYRPLNRAGMADFSSPCLQMSFETTTDFMAKAAVARSSDRLKSPSARIVMGRVGNFGTGMFDLRQP 5770
            D++++RH V E+ FGF +DD+IR LSVK++TS ++FDTL NP+ GGLYDP LGPVD  M+C TC   QK CPGH+GHIEL V VY P+LF  L  +L+ KC SCH  R S + +RV  V+++L+D G  +EA    A+LL  L       D  +GN +++     + +  +L   E   L+G+ ++      + ++ R  +  S +K M    +CENC A SP++R+D + K+F  PLS ++   NQS   ++ SA                  XXXXXXXXXX       DD  G     KV                A     A++++ P+EV  Q+Q LWK E   L+L++  G R        NG +P S++            DG+R FF++ + V P RFRPP+ MGD + EH QN+YL K++ L++ L N              +D+  G DL+  +  W ELQT VNC +DSSK  R   + P G++Q++EKKEGLFRKHMMGKRVNYA RSVISPDPY+ T+EIG+PL+FAK LT PQ V  WNVE MRQLVENG  ++PGANFVE+  G+++ L + +  +R  +   LL+              ++VWRHL  GD +L+NRQPTLHKP +MAH  RVL NP  QTIRMHYANCNTYNADFDGDE+N H PQNELA+AEA  +A  D QY+VPT+G PLRGLIQDHVD+GVK+  +D F T+  Y QL+Y A + +    G EIV        LPP I KP   WTGKQVMSTIL  LT G P+LNL+ K++     +G    EH+++FR+GELLQGVLDKG FG++EFG VHAV+E+YGS  A KLLTALGRVLT +LQ +GHTC +EDLTLT  AE  RR ++  ++  G+ +  E   + +    + A+A A   +    ++D       A++    QK     + ED D   A +D HM   +   SSDIIKACLP GQ K FP NCFSLMVLTGAKGSMVNHSQ+SC LGQQALEGRRVPV+VSGKSLPSF+A++P+PRA G+ITDRFLTG+RPQEYY HCMAGREGLVDTAVKTSRSGYLQRCL+KHLE+L VGYD+TVR+GEG V QFLYGEDG+D  Q  +LS K  QL FLA N+ +L HK GI+  F +++G D  E +  H  I  A+R   D   A+       +   + +  RR +  G   W K     GWH+A +      G    +Y + Y D G    K+P     +  SE+     PE +  SG +          LLR      +PDPV+  L +N  +G +SE  Q  ++++  +NPA L      + L   A     SP +L  L   F L++WVKYMR L  PGE VG+I AQS+GEPSTQMTLNTFHLAGHG ANVTLGIPRLREIIMTA++ + TP M VPL       AA+ +  RL+++PLS+L+ N  GI V +   +  T  LW R Y I L  F    I  AF IS KE+ R    VFV  LL+ + +++R++G          N +     + VG  SK           +  DD E +  A                          QGT++FG +KE S YG MD+E++ I               DD        G  G D      DD  D   L  ++G             + +F G   N K    E+ +R P++ + LL+V L E  A + +VRS  GI   + V+   +G       +Q  G +F+ +W+L     D +L++++L +NDI+ V + YGVEA RASI  +I  VFG YGI+VDPRHL L+AD+MT  G Y PLNR+GM    S   Q++FET+  F+  AA    +D +  PSAR+V+G+    GTG F L  P
Sbjct:    6 DQSILRHEVSEVSFGFYSDDEIRDLSVKQITSRISFDTLKNPVLGGLYDPALGPVDFNMICPTCHLTQKECPGHLGHIELPVPVYSPVLFTTLINILKRKCLSCHKFRRSSANSRVFRVRILLLDNGYVEEA----ASLLQLL-------DQKDGNFDESSTQTVQRQQAILDEFERLALSGSSKSLPRNARSVEVERDGIIASFLKGM--TNKCENCEAHSPSLRQDSNAKIFLKPLSLRSIKHNQSRAVRLSSAFDSLS----KFSTKGGGSXXXXXXXXXXDEGIIDSDDEDGD----KVT--------------TADHQGGANQYLAPLEVMSQIQLLWKHEEGLLELMW--GNRLV-----ANGRSPDSAAP----------LDGWRKFFLQVIPVAPSRFRPPVIMGDSLFEHAQNIYLAKIITLSDQLVNVSGMRRGATTNDATSDTNSGVDLSEKIYLWTELQTQVNCLIDSSKAKRP-DEVPQGIKQLIEKKEGLFRKHMMGKRVNYAARSVISPDPYISTSEIGVPLKFAKTLTLPQAVTPWNVEEMRQLVENGPDIHPGANFVENERGQLIDLSKRTHHQRVAIGKTLLTRSASAHGAHQEHKVKRVWRHLHSGDVVLMNRQPTLHKPSMMAHVTRVLTNPAMQTIRMHYANCNTYNADFDGDEMNMHFPQNELARAEAYTIANNDNQYIVPTDGSPLRGLIQDHVDSGVKLTQRDTFLTKEMYIQLVYSAWACMDHERGAEIV-------VLPPTIWKPTPLWTGKQVMSTILNLLTAGKPKLNLNAKAKIKADLYGPQNAEHVVVFRDGELLQGVLDKGQFGATEFGWVHAVYELYGSGTAAKLLTALGRVLTCYLQYAGHTCALEDLTLTEAAEAERRRLVQHAVVHGEIAYSEFAGLTDVAAARKAEADAKHDSVRLMNDDE-----RAQIRDTMQKK----MAEDLDQTSAGLDSHMMGFVHGSSSDIIKACLPHGQNKSFPANCFSLMVLTGAKGSMVNHSQISCGLGQQALEGRRVPVLVSGKSLPSFEAYDPNPRAGGYITDRFLTGLRPQEYYHHCMAGREGLVDTAVKTSRSGYLQRCLIKHLEDLHVGYDHTVRNGEGTVVQFLYGEDGIDPMQAAFLSGKPDQLSFLAMNHASLTHKFGISGEFLENSGLDLVEPAHVHQRIKDAKRYGYDRVSASVGLEMHSMKAGLTIQARR-LKAGASKWKKGSFELGWHTAVIEHVDTTGEYP-VYDILYVDTGMTAYKVPQYKRFKA-SETIT---PELSYLSGTVQ---------LLRPI----VPDPVMHQLPLNAHIGVISEKTQLEIESYAKTNPAGLLESKKTKALPVEA----QSPMMLSPL--GFRLMVWVKYMRSLCQPGENVGTICAQSIGEPSTQMTLNTFHLAGHGAANVTLGIPRLREIIMTASQKMSTPMMTVPLLATTSPAAAQQVEQRLNQVPLSELIRNVKGIRVTDEFKESATRVLWVREYTIRLSFFKAKAIRSAFGISMKEVHRSFGRVFVSRLLTLLKHEIRKSGVALSVSRTIDNDMDNASPSHVGRFSK-----------QGNDDNEDDEEA--------------------------QGTMRFGAKKEASGYGDMDDEDQQILAQQL----------DDQPAVATPSG--GSDTENSSDDDADDAAPLKPVAGELDPVAVSETVRKSPYFAGAGFNSKEQFGELKLRFPANFKTLLLVPLIEKVANQVLVRSCPGISRCYQVKQRFNGSDVEEQCIQTAGLNFQAIWEL-----DDVLDVNRLATNDIYQVLRHYGVEATRASISKQIQDVFGVYGISVDPRHLNLLADYMTHHGDYMPLNRSGMMRKGSSFQQITFETSMKFLTAAATGNLADDMDGPSARLVLGQPVKLGTGSFSLLTP 1789          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16194.1 vs. uniprot
Match: A0A1V9ZTF5_9STRA (DNA-directed RNA polymerase subunit n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9ZTF5_9STRA)

HSP 1 Score: 1375 bits (3559), Expect = 0.000e+0
Identity = 839/1910 (43.93%), Postives = 1117/1910 (58.48%), Query Frame = 2

Query:  152 SHDRTVIRHAVEEIGFGFLTDDDIRRLSVKRLTSPVTFDTLNNPLPGGLYDPVLGPVDKMMMCETCGQDQKNCPGHMGHIELAVSVYHPILFGALYKLLRAKCFSCHNLRLSKSKTRVAAVKMMLVDAGRAQEALELDATLLGYLKDGARDHDVLEGNTEDTLVSATKYRDHVLGCLEEDLAGAPRAEWCGGHNRQL---RRQLAESLIKAMGACKRCENCGAFSPNIRKDGSNKLFQVPLSDKNRMANQSCNTQIVSAVQRRDPGKPNEDXXXXXXXXXXXXXXXXXXXXXXXDDVGGSGPSSKVVGVPLAAAQAAAGDPAAKTGAQAHKFMPPIEVELQMQQLWKQESSTLDLIFVSGRRTRGVPGDVNGAPSSSSQNADGLSGGGIADGYRLFFVRALAVPPPRFRPPMNMGDMIAEHPQNVYLTKVLNLNESLKNADSLKGE------DLASVLTTWVELQTTVNCYMDSSKDPRGLKDTPPGLRQVLEKKEGLFRKHMMGKRVNYACRSVISPDPYLGTTEIGIPLRFAKELTYPQPVADWNVEAMRQLVENGTSVYPGANFVEDSSGRMLHLDRLSDLKRRGVAARLL----SSPG-------QKVWRHLTDGDCMLVNRQPTLHKPGIMAHRVRVLRNPGYQTIRMHYANCNTYNADFDGDEINCHLPQNELAKAEANLLAFTDEQYLVPTNGKPLRGLIQDHVDAGVKMCSKDCFFTRGEYQQLLYQALSGLPGLEIVPPSDDIATLPPAILKPQQRWTGKQVMSTILRHLTKGLPQLNLDGKSRTPKVAFGEAEQEHMIIFREGELLQGVLDKGAFGSSEFGLVHAVHEVYGSTAAGKLLTALGRVLTIFLQSSGHTCGIEDLTLTARAEGNRREIINKSLGIGQRSMRELVDNAGEGQNAKAIANGSATEKGDEDTGEALVEAEVEGIRQKTASFLLGEDRDSRLAEIDRHMQSALAPVSSDIIKACLPGGQAKPFPHNCFSLMVLTGAKGSMVNHSQVSCALGQQALEGRRVPVMVSGKSLPSFQAFEPSPRANGFITDRFLTGIRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEELKVGYDNTVRDGEGCVHQFLYGEDGVDTTQTKYLSSK--QLGFLAQNYQALRHKHGITPSFKKDAGFDSREASRAHSEIATAQRRRDDAAGAASASGAKFVAGEMVLAKRRAVLKGGEG-WGKAELLAGWHSAEVVKARNAGTSAALYSVRYKD-DGAMG----KKMPVSAAQEGPSESFADPSPEENNESGHLSSRRRKRPRRLLRKCPGAGLPDPVLSTLSVNKDLGCVSEAFQKSLQAFLDSNPASLRGDHTKENLKAVAATDTSSPPVLGSLKEAFELLMWVKYMRCLANPGETVGSIAAQSVGEPSTQMTLNTFHLAGHGGANVTLGIPRLREIIMTAAKNLKTPSMVVPLREGADRPAAKSLALRLSRLPLSQLLDNRGGIVVRERLVKGDTG-LWERHYAISLKLFPPSVINRAFDISFKELCRKISGVFVPNLLSAIANDLRRTGNKLSQTGGASVGLPSKGRGKRADGDRVEEEDDLEGERSAIAQSKAVAKKASKAEXXXXXXXXXXEQGTLKFGKQKEQSSYGGMDEEEKAIWKAMSGKEAGGGVLGDDDHXXXXXEGR-----EGVDGVGKDKDDDRDYFNLPNLSGARHFKGITKNKKTGEVEVTVRLPSSMRRLLMVGLAESAAAKTMVRSHKGIRGAFVVETTVDGKSGL---AVQLEGSDFETVWQLKEGGVDGILNLSKLTSNDIWAVCQTYGVEAARASIVAEITGVFGAYGIAVDPRHLGLVADHMTFDGGYRPLNRAGMADFSSPCLQMSFETTTDFMAKAAVARSSDRLKSPSARIVMGRVGNFGTGMFDLRQP 5770
            S D+T++RH V ++ FGF +DD++R LSVK++TS V+FD L N + GGLYDP LGP+D  M+C TC   QK CPGH+GHIEL V VY P+LF  +  +L+ KC SCH  R + S +R+  V+++L+D G  +EA    A +L  L+         +GN E+   +    +  +L   E  LA +  A++   + R +   R  +  + +K M    +CENC A SP +R+D   K+F  PLS +++  NQ+    + SA      G+P  D                       DD                       D  A T    H+++ P+EV  Q+Q LW+ E   ++L++              G  +++S   D L      DG+R FF+  + V P RFRPP+ MGD   EH QNVYL+K++ L+E +    S +G+      +L+  +  W ELQT VNC +D+SK  R   D P G++Q++EKKEGLFRKHMMGKRVNYA RSVISPDPY+ T+EIG+PLRFAK LT PQ V  WNV  MRQLVENG  V+PGAN+VE+  G+++ L + +  +R  +   LL    S+ G       ++VWRHL  GD +L+NRQPTLHKP +MAH  RVL NP  QTIRMHYANCNT+NADFDGDE+N H PQNELA+AEA  +A  D QYLVPT+G PLRGLIQDHVD+GVK+  +D F T+  Y QL+Y A + L G + V     + TLPP ILKP+  WTGKQVMSTIL  LT G P LNLD K++     +G    EH+IIFR+GELLQGVLDK  FG+SE+G VHA +E+YGS  A KLLTALGRVLT +LQ +GHTC +EDLTLT  AE  RR ++ +S+  G+ +  E                GS  E    DT   L + E   +R+ T    + ED ++  + +D HM   +   +S+IIK+CLP GQ K FP NCFSLMVLTGAKGSMVNHSQ+SC LGQQALEGRRVPV+VSGKSLPSF+AF+PSPRA G+ITDRFLTG+RPQEYY HCMAGREGLVDTAVKTSRSGYLQRCLVKHLE+L V YD+TVR+ +G V QFLYGEDGVDT    +LS K  Q+ FLA N+ +L HK G+   F + +G D    ++ H EI TA+R   +  GA      K   G  VLA+R   LK GE  W K     GWH+A +     +G    +Y + Y D + A G    K +  SAA            PE +  SG +          L+R      LPDPV+  L +N  +GC+SE  Q  ++A+  +NP++L  +  K  L  V +              AF L++WVKYMR L  PGE VG+I AQS+GEPSTQMTLNTFHLAGHG ANVTLGIPRLREIIMTA+K + TP M +PL   A   A  ++  +L+++PL++L+ N  GI V+++ ++ +   LW R Y + L  F P +I  AF I+ KE+ R     FV  LL+ + +++R++G  +S   GA     +   G           DD                 A              +QGTLKFG +KE + YG MD+EE  +                   XXXXX        E    +   K + +       ++   +F G   N      E+ +R P++ + LL+V L E  A++ +VRS  GI   ++ +  +D KSG     +Q  G +F+ +W+  +     +L++++L +NDI+ V   YGVEAARASI  +I  VFG YGI+VDPRHL L+AD+MT  G Y PLNR+GMA   S   Q++FET+  F+ +AA     D +  PSAR+V+G+    GTG F L  P
Sbjct:    4 SMDQTILRHEVADVSFGFYSDDEVRALSVKQITSRVSFDALKNAVIGGLYDPALGPIDFNMICPTCHMTQKECPGHLGHIELPVPVYSPVLFATMLNILKRKCMSCHKFRKAASVSRIYRVRLLLLDNGYIEEA----AQMLELLEQK-------DGNFEEGHAATVARQQAILDEFER-LALSGAAKYLPKNPRAVEVERENIINTFLKGM--TNKCENCEAHSPGLRQDSHAKIFLRPLSLRSQRHNQARAIHLHSAFDTMHKGQPAGDDSDDEDME---------------DD-----------------------DAEAPTSNTGHQYLAPLEVMSQLQLLWQHEDGIMELMW--------------GDRAAASARGDKLE----LDGWRKFFLHVIPVAPSRFRPPVIMGDQQFEHSQNVYLSKIIALSEQMVTMSSARGDPEATAVNLSDKIYLWTELQTQVNCLIDNSKAKRP-DDMPQGIKQLIEKKEGLFRKHMMGKRVNYAARSVISPDPYISTSEIGVPLRFAKTLTLPQAVTPWNVVEMRQLVENGPDVHPGANYVENERGQLIDLSKRTPQQRAAIGKTLLTRSASAQGAHQKHKVKRVWRHLKSGDVVLMNRQPTLHKPSMMAHVTRVLTNPAMQTIRMHYANCNTFNADFDGDEMNMHFPQNELARAEAYHIANNDNQYLVPTDGSPLRGLIQDHVDSGVKLTQRDTFLTKDMYLQLVYSAWTCLDGEKGV-----VRTLPPTILKPEPLWTGKQVMSTILLMLTAGKPSLNLDSKAKIKADLYGPGNAEHIIIFRDGELLQGVLDKAQFGASEYGWVHACYELYGSGTAAKLLTALGRVLTCYLQYAGHTCALEDLTLTEPAEVERRRLVEQSVLGGELAYTEFA------------GLGSLVEARKSDTPRRLNDEERAAVRE-TMRRKMAEDLENTSSALDSHMMGFVHGSNSEIIKSCLPHGQNKSFPANCFSLMVLTGAKGSMVNHSQISCGLGQQALEGRRVPVLVSGKSLPSFEAFDPSPRAGGYITDRFLTGLRPQEYYHHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEDLHVAYDHTVRNCDGNVVQFLYGEDGVDTLNAAFLSGKADQMSFLAMNHASLSHKFGLNADFLETSGLDVVAPAQLHHEIKTAKRHGYET-GALELQSMKV--GLQVLARR---LKAGERKWKKGHFALGWHAATITGVDASGDYP-VYDLTYADGETATGVPQYKHLKASAAA----------MPENSFMSGSVE---------LIR----PQLPDPVMHALPLNSHIGCISEKSQGHIEAYSKANPSNLL-ESKKNKLGGVVSAS------------AFRLMVWVKYMRSLCQPGENVGTICAQSIGEPSTQMTLNTFHLAGHGAANVTLGIPRLREIIMTASKKMSTPMMTIPLMPSASAAAGMAVEQQLNQVPLAELIRNTKGIRVQDQFLEAENRVLWCREYTVRLYFFKPKLIRDAFGITSKEIQRAFGRSFVSKLLTLLKHEMRKSGVTVSVESGAVSARSTDNLG-----------DDXXXXXXXXXXXXXXXXXAD-------------DQGTLKFGAKKEAAGYGDMDDEELEMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEDAAPIKVLKGELKPVNVTDTVNKNPYFAGAGVNTTEHYGELKLRFPANFKTLLLVPLIEKVASQVLVRSCPGISRCYMTKQRLD-KSGAEEQCIQTAGLNFQAIWERND-----VLDVNRLVTNDIYQVLLHYGVEAARASISKQIQDVFGVYGISVDPRHLSLLADYMTHYGDYMPLNRSGMARKGSSFQQITFETSMKFLTQAATGGFGDDMAGPSARLVLGQPVKLGTGSFSLMTP 1751          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16194.1 vs. uniprot
Match: A0A5D6XPF2_9STRA (DNA-directed RNA polymerase subunit n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XPF2_9STRA)

HSP 1 Score: 1372 bits (3550), Expect = 0.000e+0
Identity = 847/1968 (43.04%), Postives = 1139/1968 (57.88%), Query Frame = 2

Query:  158 DRTVIRHAVEEIGFGFLTDDDIRRLSVKRLTSPVTFDTLNNPLPGGLYDPVLGPVDKMMMCETCGQDQKNCPGHMGHIELAVSVYHPILFGALYKLLRAKCFSCHNLRLSKSKTRVAAVKMMLVDAGRAQEALELDATLLGYLKDGARDHDVLEGNTEDTLVSATKYRDHVLGCLEEDLA-GAPRAEWCGGHNRQL-------RRQLAESLIKAMGACKRCENCGAFSPNIRKDGSNKLFQVPLSDKNRMANQSCNTQIVSAVQRRDPGKPNEDXXXXXXXXXXXXXXXXXXXXXXXDDVGGSGPSSKVVGVPLAAAQAAAGDPAAKTGAQAHKFMPPIEVELQMQQLWKQESSTLDLIFVSGRRTRGVPGDVNGAPSSSSQNADGLSGGGIADGYRLFFVRALAVPPPRFRPPMNMGDMIAEHPQNVYLTKVLNLNESLKNADSLKGE------------------------DLASVLTTWVELQTTVNCYMDSSKDPRGLKDTPPGLRQVLEKKEGLFRKHMMGKRVNYACRSVISPDPYLGTTEIGIPLRFAKELTYPQPVADWNVEAMRQLVENGTSVYPGANFVEDSSGRMLHLDRLSDLKRRGVAARLL----SSPG------QKVWRHLTDGDCMLVNRQPTLHKPGIMAHRVRVLRNPGYQTIRMHYANCNTYNADFDGDEINCHLPQNELAKAEANLLAFTDEQYLVPTNGKPLRGLIQDHVDAGVKMCSKDCFFTRGEYQQLLYQALSGLPGLEIVPPSDDIATLPPAILKPQQRWTGKQVMSTILRHLTKGLPQLNLDGKSRTPKVAFGEAEQEHMIIFREGELLQGVLDKGAFGSSEFGLVHAVHEVYGSTAAGKLLTALGRVLTIFLQSSGHTCGIEDLTLTARAEGNRREIINKSLGIGQRSMRE---LVDNAGEGQNAKAIANGSATEKGDEDTGEALVEAEVEGIRQKTASFLLGEDRDSRLAEIDRHMQSALAPVSSDIIKACLPGGQAKPFPHNCFSLMVLTGAKGSMVNHSQVSCALGQQALEGRRVPVMVSGKSLPSFQAFEPSPRANGFITDRFLTGIRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEELKVGYDNTVRDGEGCVHQFLYGEDGVDTTQTKYLSSK--QLGFLAQNYQALRHKHGITPSFKKDAGFDSREASRAHSEIATAQRRRDDAAGAASASGAKFVAGEMVLAKRRAVLKGGEG-WGKAELLAGWHSAEVVKARNAGTSA--ALYSVRYKDDGAMGKKMPVSAAQEGPSESFADPSPEENNESGHLSSRRRKRPRRLLRKCPGAGLPDPVLSTLSVNKDLGCVSEAFQKSLQAFLDSNPAS----LR-----------------------GDHTKENL--KAVAATDTSSPPVLGSLKEAFELLMWVKYMRCLANPGETVGSIAAQSVGEPSTQMTLNTFHLAGHGGANVTLGIPRLREIIMTAAKNLKTPSMVVPLREGADRPAAKSLALRLSRLPLSQLLDNRGGIVVRERLVKGDTG-LWERHYAISLKLFPPSVINRAFDISFKELCRKISGVFVPNLLSAIANDLRRTGNKLSQTGGASVGLPSKGRGKRADGDRVEEEDDLEGERSAIAQSKAVAKKASKAEXXXXXXXXXXEQGTLKFGKQKEQSSYGGMDEEEKAIWKAMS-----------GKEAGGGV---LGDDDHXXXXXEGREGVDGVGKDKDDDRDYFNLP-NLSGARHFKGITKNKKTGEVEVTVRLPSSMRRLLMVGLAESAAAKTMVRSHKGIRGAFVVETTVDGKSGLA--VQLEGSDFETVWQLKEGGVDGILNLSKLTSNDIWAVCQTYGVEAARASIVAEITGVFGAYGIAVDPRHLGLVADHMTFDGGYRPLNRAGMADFSSPCLQMSFETTTDFMAKAAVARSSDRLKSPSARIVMGRVGNFGTGMFDLRQP 5770
            D+T++R+ V E+ FGF +D +IR LSVK++TS ++FD LNNP+ GGLYDP LGPVD  M+C TC Q QK CPGH+GHIEL V VY+P+LFG +  LL+ KCF+CH  R + +++RV  VK++L+D+G   EA    ATL   L+             ED     T  R   +    E LA     A+  G   RQL       R +LA    K + A  +CENCGA SP +R+D   K+F   LS ++R AN+S N  + SA+         ++                       DD   SG  S++     AA++    D ++++     KF+PP+EV+ Q+Q +WK E   ++L++          GD N A   ++  ADG         +R FF+  + V P RFRPP+ +GD   EH QN YL+K+L L+E++      + +                        +LA  L  W ELQ  VNC +DSS+   G  D   G++QV+EKKEGLFRK+MMGKRVNYA RSVISPDPY+ T++IG+PLRFAK LTYPQPV  WNVE MRQLV NG  V+PGANFVE  SGR++ L R S  +R  ++  LL    S+ G      ++VWRHL  GD +L+NRQPTLHKP +MAH  RVL NP  QTIRMHYANCNT+NADFDGDE+N H PQNELA+AEA  +A  D QYLVPT+G PLRGLIQDHVD+GVK+  +D F T+  Y QLLY A + + G      +  I  +PPAILKPQ  WTGKQV++++L+ LTKGLP LNLD K++     +G    EH+++FR+GELLQGVLDK  FG+S  G VHA +EVYGS  A  LLT LGR+ T +LQ +GHTC +EDLTLT  AE  RR+++++S+  G+ +  E   L D     ++ +    GS   +        + E E   IR++  + L G D D     +D HM   +   +SDIIK CLP GQ K FP N FSLMVLTGAKGSMVNHSQ+SC LGQQALEGRRVP++ SG+SLPS++ F+P+PRA G+ITDRFLTG+RPQEYY HCMAGREGLVDTAVKTSRSGYLQRCL+KHLE+L+V YD+TVR  +G V QFLYGEDGVD   +  LS K  +  F A N++++ HK+GI   F + +  D  +  + H+E   AQ      A      G+   AG  V A+R   LK GE  W +  +  G+ +AEV+           + Y ++Y D G     +P +         F    P   N +  +S R       LL+    A   DPV+ +L +N+ +GCVSE  Q  L+A+ + NPA+    LR                       GD   + +  + +    T SP       +AF+LL+WV Y+R +ANPGE VG IAAQS+GEPSTQMTLNTFHLAGHG ANVTLGIPRLREIIMTA++ + TP M +PL        A  +  RL+++ L++LL N  G+ VR+  V  + G LW R Y I L LF    I RAF +S  ++   +   F+  LLS I+ +++++G  ++    A+       RG  A                                  XXXXXXX EQGTL+FG ++E   Y  MD+E++ + +A              K+A G     +G  D        +    G+        +  ++P ++   ++F    +N K   VE+ +R P+  + +LMV L E  A + +V+   GI   +++   +         VQ EG +F+ +W       D IL++++L +NDI+ V QTYGVEAARASI  +I  VFG YGI+VDPRHL L+AD+MT  GGY PLNR GM +  S   Q++FET+  F+ +AA+A +SD L+SPSARI++G+    GTG F L QP
Sbjct:    6 DQTILRYEVAEVAFGFYSDAEIRELSVKQVTSRLSFDALNNPVVGGLYDPALGPVDFHMICPTCHQTQKECPGHLGHIELPVPVYNPVLFGHMLTLLKRKCFTCHKFRSASARSRVLRVKLLLIDSGFDDEA----ATLAELLEQ--------RNGVEDEPQQQTFRRQQAILDEYERLALSQAGAQQRGQRLRQLARLSEVQREKLAAEFFKGLKA--KCENCGAISPGLRQDAKAKIFLRGLSARSRKANRSKNLTVTSALDTMHGNVSGDERMGS-------------------DDSDDSGSESELEDGKFAASE----DTSSRS-----KFLPPLEVQSQLQLMWKNEDGLMELLY----------GDRNVASGRAAAPADG---------WRKFFLNVIPVAPSRFRPPVFLGDKQFEHAQNSYLSKILTLSENIVQGSYYEQQKLPRAGAEDGXXXXXXXXXTAPQVNLARKLAVWTELQVNVNCLVDSSQAKAGA-DAAQGIKQVIEKKEGLFRKNMMGKRVNYAARSVISPDPYISTSQIGVPLRFAKTLTYPQPVTPWNVEEMRQLVINGPDVHPGANFVESESGRLIDLKRRSAHQREAISKTLLTRSASAQGTSQHRVKRVWRHLHTGDIVLMNRQPTLHKPSMMAHVTRVLTNPAMQTIRMHYANCNTFNADFDGDEMNMHFPQNELARAEAYHIAANDHQYLVPTDGSPLRGLIQDHVDSGVKLSQRDTFLTKDLYMQLLYNAWASMEGAGAA--AAHIEVVPPAILKPQALWTGKQVITSVLKMLTKGLPPLNLDSKAKIKGDLYGATNAEHVVVFRDGELLQGVLDKSQFGASMHGFVHACYEVYGSRVAADLLTTLGRLFTCYLQFAGHTCALEDLTLTKDAERRRRKLVDESVLNGEEAYAEFAGLTDLLAAKRSEEEATPGSRGRR-------RMNETERAQIRERMRALLAGPDADQNAKALDAHMMGTVHGSNSDIIKTCLPAGQTKAFPANGFSLMVLTGAKGSMVNHSQISCGLGQQALEGRRVPILSSGRSLPSYEPFDPAPRAGGYITDRFLTGLRPQEYYHHCMAGREGLVDTAVKTSRSGYLQRCLMKHLEDLQVSYDHTVRGADGSVVQFLYGEDGVDPLMSAMLSGKDAEFSFQAMNHRSISHKYGIDAQFFERSKLDIMKPLKLHAEARAAQ------AHGFEQPGSLLRAGVRVQARR---LKAGETKWKRGNIERGFFAAEVLALAQEYEDPRDSTYDIQYVDTGLKAFNVPRTTV-------FKARKP--TNHTRAMSGRVE-----LLK----AERADPVMQSLELNQHVGCVSERIQDKLRAYSERNPANCLDLLRAKKSXXXXXXXXXXXXXKTAEQSGDDGADEIDREKMRTKHTVSP-------DAFQLLVWVNYLRSMANPGENVGVIAAQSIGEPSTQMTLNTFHLAGHGAANVTLGIPRLREIIMTASEKMSTPMMTIPLLAHVPAARAHEVEQRLNQVALAELLSNERGVAVRDEFVPSENGILWVRDYTIRLTLFKLKQIKRAFGLSADDVFGAVGKNFIGKLLSFISREMKKSGVSVAAAAEATNNFAGSTRGTNAGXXXX-----------------------------XXXXXXXDEQGTLRFGSRREVQGYE-MDDEDEQLRQAQDDSDXXXXXXARSKKARGAKRASVGPADEEATGSGAKSKKSGMATALAGGLEALDVPMSVRKNKYFVYCGRNTKAHYVELRLRFPTHSKTVLMVPLVERVAKQVLVQHCPGISRCYLINQRLGDAQDETPCVQTEGLNFQEIWAF-----DDILDVNQLATNDIFQVLQTYGVEAARASISKQINDVFGVYGISVDPRHLSLLADYMTAYGGYMPLNRMGMNNKGSALQQITFETSMKFLTQAALAGNSDNLESPSARIILGQPPRVGTGSFSLLQP 1833          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16194.1 vs. uniprot
Match: K3X8S6_GLOUD (DNA-directed RNA polymerase subunit n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3X8S6_GLOUD)

HSP 1 Score: 1367 bits (3537), Expect = 0.000e+0
Identity = 842/1963 (42.89%), Postives = 1129/1963 (57.51%), Query Frame = 2

Query:  158 DRTVIRHAVEEIGFGFLTDDDIRRLSVKRLTSPVTFDTLNNPLPGGLYDPVLGPVDKMMMCETCGQDQKNCPGHMGHIELAVSVYHPILFGALYKLLRAKCFSCHNLRLSKSKTRVAAVKMMLVDAGRAQEALELDATLLGYLKDGARDHDVLEGNTEDTLVSATKYRDHVLGCLEEDLAGAPRAEWCGGHNRQL-------RRQLAESLIKAMGACKRCENCGAFSPNIRKDGSNKLFQVPLSDKNRMANQSCNTQIVSAVQRRDPGKPNEDXXXXXXXXXXXXXXXXXXXXXXXDDVGGSGPSSKVVGVPLAAAQAAAGDPAAKTGAQAHKFMPPIEVELQMQQLWKQESSTLDLIFVSGRRTRGVPGDVNGAPSSSSQNADGLSGGGIADGYRLFFVRALAVPPPRFRPPMNMGDMIAEHPQNVYLTKVLNLNESLKN---------------------ADSLKGEDLASVLTTWVELQTTVNCYMDSSKDPRGLKDTPPGLRQVLEKKEGLFRKHMMGKRVNYACRSVISPDPYLGTTEIGIPLRFAKELTYPQPVADWNVEAMRQLVENGTSVYPGANFVEDSSGRMLHLDRLSDLKRRGVAARLL----SSPG------QKVWRHLTDGDCMLVNRQPTLHKPGIMAHRVRVLRNPGYQTIRMHYANCNTYNADFDGDEINCHLPQNELAKAEANLLAFTDEQYLVPTNGKPLRGLIQDHVDAGVKMCSKDCFFTRGEYQQLLYQALSGLPGLEIVPPSDDIATLPPAILKPQQRWTGKQVMSTILRHLTKGLPQLNLDGKSRTPKVAFGEAEQEHMIIFREGELLQGVLDKGAFGSSEFGLVHAVHEVYGSTAAGKLLTALGRVLTIFLQSSGHTCGIEDLTLTARAEGNRREIINKSLGIGQRSMRELVDNAGEGQNAKAIANGSATEKGDEDTGEALVEAEVEGIRQKTASFLLGEDRDSRLAEIDRHMQSALAPVSSDIIKACLPGGQAKPFPHNCFSLMVLTGAKGSMVNHSQVSCALGQQALEGRRVPVMVSGKSLPSFQAFEPSPRANGFITDRFLTGIRPQEYYFHCMAGREGLVDTAVKTSRSGYLQRCLVKHLEELKVGYDNTVRDGEGCVHQFLYGEDGVDTTQTKYLSSK--QLGFLAQNYQALRHKHGITPSFKKDAGFDSREASRAHSEIATAQRRRDDAAGAASASGAKFVAGEMVLA-----KRRAVLKGGEGWGKAELLAGWHSAEVVKARNAGTSA--ALYSVRYKDDGAMGKKMPVSAAQEGPSESFADPSPEENNE--SGHLSSRRRKRPRRLLRKCPGAGLPDPVLSTLSVNKDLGCVSEAFQKSLQAFLDSNPASL------------------RGDHTKENL--KAVAATDTSSPPVLGSLKEAFELLMWVKYMRCLANPGETVGSIAAQSVGEPSTQMTLNTFHLAGHGGANVTLGIPRLREIIMTAAKNLKTPSMVVPLREGADRPA-AKSLALRLSRLPLSQLLDNRGGIVVRERLVKGDTG-LWERHYAISLKLFPPSVINRAFDISFKELCRKISGVFVPNLLSAIANDLRRTGNKLSQTGGASVGLPSKGRGKRADGDRVEEEDDLEGERSAIAQSKAVAKKASKAEXXXXXXXXXXEQGTLKFGKQKEQSSYGGMDEEEKAIWKAMSGKE---------------AGGGVLGDDDHXXXXXEGREGVDGVGKDKD---DDRDYFNLP-NLSGARHFKGITKNKKTGEVEVTVRLPSSMRRLLMVGLAESAAAKTMVRSHKGIRGAFVVETTVDG--KSGLAVQLEGSDFETVWQLKEGGVDGILNLSKLTSNDIWAVCQTYGVEAARASIVAEITGVFGAYGIAVDPRHLGLVADHMTFDGGYRPLNRAGMADFSSPCLQMSFETTTDFMAKAAVARSSDRLKSPSARIVMGRVGNFGTGMFDLRQP 5770
            D+T++R+ V E+GFGF +D +IR LSVK++TS ++FD+LNNP+ GGLYDP +GPVD  M+C TC Q QK CPGH+GHIEL V VY+P+LFG +  LL+ KCF+CH  R++ +++RV  VK++L+D G  +EA  L   L                  ED     T  R   +    E LA +      GG  R L       R +LA    K + A  +CENCGA SP IR+D ++K+F   LS ++R  N+S N  + SA+   +    + D         XXXXXXX            S   SK       +++A              K++PP+EV+ Q+Q +WK E   ++L+F          GD N A     Q          ADG+R FF+  + V P RFRPP+ +GD   EH QN YL+K+L L+ES+ +                     AD  K  +LA  L  W ELQ  VN  +DSSK   G +D   G++QV+EKKEGLFRKHMMGKRVNYA RSVISPDPY+ T++IG+PLRFAK LTYPQPV  WNVE MRQLV NG  V+PGANFVE  +GR++ L + +  +R  ++  LL    S+ G      ++VWRHL  GD +L+NRQPTLHKP +MAH  RVL NP  QTIRMHYANCNT+NADFDGDE+N H PQNELA++EA  +A  D QYLVPT+G PLRGLIQDHVD+GVK+  +D F T+  Y QLLY A + +    +      I  +PPAILKP+Q WTGKQV++++L+ LTKGLP LNLD K++     +G    EH+++FR+GELLQGVLDK  FG+S  G VHA +E+YG+  A  LLT LGR+ T +LQ +GHTC +EDLTL   AE  RR+++N S+  G+ +  E       G      A   + EK        + E E E IR++    L G D ++    +D HM   +   +SDIIK CLP GQ K FP NCFSLMVLTGAKGSMVNHSQ+SC LGQQALEGRRVP++ SG+SLPS++ F+P+PRA G+ITDRFLTG+RPQEYY HCMAGREGLVDTAVKTSRSGYLQRCL+KHLE+L+V YD+TVR  EG V QFLYGEDG+D   +  LS K  +  F A N++++ HK+GI   F      D  +  + H+E             A  A+  K+ A   VLA     + R + +G   W +  +  G+  AE++           + Y ++Y D G     +P +         F    P  N    SG +          L+         DPV+  L +N+ +GC+SE  Q  L  +   NPA+                    GD   E +  + +    T SP       +AF+LL+WV Y+R +ANPGE VG + AQS+GEPSTQMTLNTFHLAGHG ANVTLGIPRLREIIMTA++ + TP M +PL +     A A+ +  RL+++ LS+L+ N+ G+ V++  +K + G LW R Y I L LF    I +AF ++  ++   I   F+  LLS ++ +++++G  +S                        E ++  G  S+                XXXXXXXXXEQGTL+FG + E + YG MDEE+K I  A                    AGG   GD+            + G  K K    D  +  N+P ++   ++F    +N K   VE  +R P+  + LLMV + E  A + +V+   GI   +++   +    +    +Q EG +F+ +W       D IL++++L +NDI+ V QTYGVEAARASI  +I  VFG YGI+VDPRHL L+AD+MT  GGY PLNR GM +  S   Q++FET+  F+ +AA+   SD L+SPSARI++G+    GTG F L QP
Sbjct:    6 DQTILRYEVSEVGFGFYSDAEIRDLSVKQITSRMSFDSLNNPVIGGLYDPAMGPVDFNMICPTCHQTQKECPGHLGHIELPVPVYNPVLFGHMLNLLKRKCFTCHKFRIASARSRVLRVKILLIDNGFDEEARSLSELL------------EQRNGVEDEPPQRTFQRQQAILDEFERLALSQVNVANGGKIRPLPRSSEVMREKLATEFFKGVKA--KCENCGAISPGIRQDSNSKIFLKGLSARSRKVNRSKNLTVTSALDTMNGNVSDNDARMGSDDSDXXXXXXX------------SNGKSKYAMTEDTSSRA--------------KYLPPLEVQSQLQLMWKNEDGLMELLF----------GDRNVASGRIPQAK--------ADGWRKFFLNVIPVAPSRFRPPVFLGDKQFEHAQNSYLSKILTLSESIVHGNYYDQXXXXXXXXXXXXXXXADEKKQINLARKLAVWTELQNNVNMLVDSSK---GNQDAAQGIKQVIEKKEGLFRKHMMGKRVNYAARSVISPDPYISTSQIGVPLRFAKTLTYPQPVTPWNVEEMRQLVINGPDVHPGANFVESETGRLIDLKKRTLHQREAISKTLLTRSASAQGTSKNRVKRVWRHLKTGDVVLMNRQPTLHKPSMMAHVTRVLTNPKMQTIRMHYANCNTFNADFDGDEMNMHFPQNELARSEAYNIAANDHQYLVPTDGSPLRGLIQDHVDSGVKLSQRDTFLTKEMYMQLLYSAWASMEDAGVE--KAHIEIVPPAILKPKQLWTGKQVITSVLKLLTKGLPPLNLDSKAKIKGDLYGNGNAEHIVVFRDGELLQGVLDKSQFGASMHGFVHACYEIYGARIAADLLTTLGRLFTCYLQFAGHTCALEDLTLNKDAEKRRRKLVNDSVFNGEEAYAEFA-----GLTELLAAKHESEEKEHGGKKRRMNEQEREQIRERMRELLSGTDAENNAKALDAHMMGTVHGSNSDIIKTCLPSGQTKSFPANCFSLMVLTGAKGSMVNHSQISCGLGQQALEGRRVPILCSGRSLPSYEPFDPAPRAGGYITDRFLTGLRPQEYYHHCMAGREGLVDTAVKTSRSGYLQRCLMKHLEDLQVNYDHTVRGCEGNVVQFLYGEDGIDPVLSAMLSGKDSEFNFQAMNHRSISHKYGIDAKFFAHTKLDIMKPLKLHAE-------------AREATSKKYFAPTSVLAPGLRVQARRLKQGETKWKRGNIEKGFFDAEILALSQEYEDGRDSTYDIQYLDTGLKAFGVPRTTV-------FKSRKPTNNTRAMSGRVD---------LIM----VARADPVMQELELNQHVGCISERIQNKLWQYNQRNPANCLDLKKKSXXXXXXXXXXHSGDDDVEEIDREKMMTKHTVSP-------DAFQLLVWVNYLRSMANPGENVGVLCAQSIGEPSTQMTLNTFHLAGHGAANVTLGIPRLREIIMTASEKMSTPMMTIPLLKNDKAQARAQEVEQRLNQVALSELISNQRGVSVKDEFLKSENGILWVRDYTIRLTLFKLKYIKKAFGLTSDDVYNAIGKGFIQKLLSVLSREMKKSGVSVSAI----------------------ETNNFAGFGSSXXXXXXXX--------XXXXXXXXXEQGTLRFGSRGEVNGYGEMDEEDKKIQNAQDSXXXXXXXXXXXXXXXXTAGGNTSGDEGDAMDSSN----MSGSKKTKSFVADGLESLNVPISVRKNKYFMYAGRNTKEHFVEFRLRFPTHSKTLLMVPIVERVAKQVLVQHCPGISRCYLINQRIGDAQEETPCIQTEGLNFQEIWSF-----DDILDVNQLATNDIYQVLQTYGVEAARASISKQINDVFGVYGISVDPRHLSLLADYMTAQGGYMPLNRMGMNNKGSSMQQITFETSMKFLTQAALGGLSDNLESPSARIILGQPPRVGTGSFSLLQP 1821