mRNA_E-siliculosus-1a_M_contig8.16097.1 (mRNA) Ectocarpus siliculosus Ec864m_EcPH12_78m male

BLAST of mRNA_E-siliculosus-1a_M_contig8.16097.1 vs. uniprot
Match: D8LQI2_ECTSI (NAD(P)H-Nitrate reductase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LQI2_ECTSI)

HSP 1 Score: 1679 bits (4349), Expect = 0.000e+0
Identity = 816/827 (98.67%), Postives = 824/827 (99.64%), Query Frame = 2

Query:   23 MPPAGPGIQSSKPEKPDASLGSIKGHDYYKDTFVPPSEVVTEIDARDKGTPDDWVPRHPELVRLTGRHPFNVEPPVWRIKDHGFISPNELHYVRNHGAVPRITDGASHKVRVGGAVDKPLEMTLEYIKATFQEITIPVTLVCAGNRRKEENMIKQGLGFSWGPAAVSNAMWTGVWVKDILEHVGIKSFEKGGKHVCFVGADKLPNGYYGTSIRREVCMDETADVLLAYKMNGKELSPDHGYPIRLIVPGYIGGRMIKWLTDIEVTDLESDNHYHYFDNRVLPSQVDAEKAVAEGWWYKPPYIINELNINSAVVYPRHDEVVSLTSPETTYTVSGYAYGGGGIALTRVEVSLDDGKTWELAELSIPEKPRHMGRYWCWVFWEFKVDVLRLMKCEEIVCRAWQGQNTQPQNITWNLLGMMNNCWFRCKVHPTRDDRGALALTFEHPTMPGPQPGGWMVKGXXXXXXXXXXXXXXKTPSNGKSFNLKDVHKHDNEDDCWIAVDGKVYDVTDFLDDHPGGGESITISAGQDSSEEFNALHSDKARAMLQDYYIGDLDSSVAKPEKVVEEEKLVALNTRQKLAVPLIEKEVLSHDSRRFRFELPTKDHKLGLPIGKHFFVSGKWNGEFVMRPYTPVTGDEVSGYVDLVIKVYTPNDRFPKGGKMSQMLDALDIGDTIDIKGPVGEIVYLEPGQFLIKGKPRNANKLAMLAGGTGITPMYQVIKAVLSDPADTTLCSLIYANQTEEDILLRDELDALAKANPDRFKLWYTLDRPSGDWKYDKGFISKDMCEAHLPEASPETIAFMCGPPAMIKFACIPNLAKIGYGKDDYISF 2503
            MPPAGPGIQSSKPEKP+ASL SIKGHDYYKDTFVPPSEVVTEIDARDKGTPDDWVPRHPELVRLTGRHPFNVEPPVWRIKDHGFISPNELHYVRNHGAVPRITDGASHKVRVGGAVDKPLEMTLEYIKATFQEITIPVTLVCAGNRRKEENMIKQGLGFSWGPAAVSNAMWTGVWVKDILEHVGIKSFE+GGKHVCFVGADKLPNGYYGTSIRREVCMDETADVLLAYKMNGKELSPDHGYPIRLIVPGYIGGRMIKWLTDIEVTDLESDNHYHYFDNRVLPSQVDAEKAVAEGWWYKPPYIINELNINSA+VYPRHDEVVSLTSPETTYTVSGYAYGGGGIALTRVEVSLDDGKTWELAELS PEKPRHMGRYWCWVFWEFKVDVLRLMKCEEIVCRAWQGQNTQPQNITWNLLGMMNNCWFRCKVHPTRDDRGALALTFEHPTMPGPQPGGWMVKGXXXXXXXXXXXXXXKTPSNGKSFNLKDV KHDNEDDCWIAVDGKVYDVTDFLDDHPGGGESITISAGQDSSEEFNALHSDKARAML+DYYIGDLDSSVAKPEK+VEEE+LVALNTRQKLAVPLIEKEVLSHDSRRFRFELPTKDHKLGLPIGKHFFVSGKWNGEFVMRPYTPVTGDEVSGYVDLVIKVYTPNDRFPKGGKMSQMLDALDIGDTIDIKGPVGEIVYLEPGQFLIKGKPRNANKLAMLAGGTGITPMYQVIKAVLSDPADTTLCSLIYANQTEEDILLRDELDALAKANPDRFKLWYT+DRPSGDWKYDKGFISK+MCEAHLPEASPETIAFMCGPPAMIKFACIPNLAKIGYGKDDYISF
Sbjct:    1 MPPAGPGIQSSKPEKPNASLASIKGHDYYKDTFVPPSEVVTEIDARDKGTPDDWVPRHPELVRLTGRHPFNVEPPVWRIKDHGFISPNELHYVRNHGAVPRITDGASHKVRVGGAVDKPLEMTLEYIKATFQEITIPVTLVCAGNRRKEENMIKQGLGFSWGPAAVSNAMWTGVWVKDILEHVGIKSFEEGGKHVCFVGADKLPNGYYGTSIRREVCMDETADVLLAYKMNGKELSPDHGYPIRLIVPGYIGGRMIKWLTDIEVTDLESDNHYHYFDNRVLPSQVDAEKAVAEGWWYKPPYIINELNINSAIVYPRHDEVVSLTSPETTYTVSGYAYGGGGIALTRVEVSLDDGKTWELAELSTPEKPRHMGRYWCWVFWEFKVDVLRLMKCEEIVCRAWQGQNTQPQNITWNLLGMMNNCWFRCKVHPTRDDRGALALTFEHPTMPGPQPGGWMVKGXXXXXXXXXXXXXXKTPSNGKSFNLKDVQKHDNEDDCWIAVDGKVYDVTDFLDDHPGGGESITISAGQDSSEEFNALHSDKARAMLEDYYIGDLDSSVAKPEKIVEEEQLVALNTRQKLAVPLIEKEVLSHDSRRFRFELPTKDHKLGLPIGKHFFVSGKWNGEFVMRPYTPVTGDEVSGYVDLVIKVYTPNDRFPKGGKMSQMLDALDIGDTIDIKGPVGEIVYLEPGQFLIKGKPRNANKLAMLAGGTGITPMYQVIKAVLSDPADTTLCSLIYANQTEEDILLRDELDALAKANPDRFKLWYTIDRPSGDWKYDKGFISKEMCEAHLPEASPETIAFMCGPPAMIKFACIPNLAKIGYGKDDYISF 827          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16097.1 vs. uniprot
Match: A0A835YIE1_9STRA (Nitrate reductase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YIE1_9STRA)

HSP 1 Score: 1150 bits (2974), Expect = 0.000e+0
Identity = 551/851 (64.75%), Postives = 658/851 (77.32%), Query Frame = 2

Query:   44 IQSSKPEKPDASLGSIKGHDYYKDTFVPPSEVVTEIDARDKGTPDDWVPRHPELVRLTGRHPFNVEPPVWRIKDHGFISPNELHYVRNHGAVPRITDGASHKVRVGGAVDKPLEMTLEYIKA-TFQEITIPVTLVCAGNRRKEENMIKQGLGFSWGPAAVSNAMWTGVWVKDILEHVGIKSFEKGGKHVCFVGADKLPNGYYGTSIRREVCMDETADVLLAYKMNGKELSPDHGYPIRLIVPGYIGGRMIKWLTDIEVTDLESDNHYHYFDNRVLPSQVDAEKAVAEGWWYKPPYIINELNINSAVVYPRHDEVVSLTSP--ETTYTVSGYAYGGGGIALTRVEVSLDDGKTWELAELSIPEKPRHMGRYWCWVFWEFKVDVLRLMKCEEIVCRAWQGQNTQPQNITWNLLGMMNNCWFRCKVHPTRDDRGALALTFEHPTMPGPQPGGWMVKGXXXXXXXXXXXXXX-KTPSNGKS---FNLKDVHKHDNEDDCWIAVDGKVYDVTDFLDDHPGGGESITISAGQDSSEEFNALHSDKARAMLQDYYIGDL---DSSVAKPEKVV-----------------EEEKLVALNTRQKLAVPLIEKEVLSHDSRRFRFELPTKDHKLGLPIGKHFFVSGKWNGEFVMRPYTPVTGDEVSGYVDLVIKVYTPNDRFPKGGKMSQMLDALDIGDTIDIKGPVGEIVYLEPGQFLIKGKP-RNANKLAMLAGGTGITPMYQVIKAVLSDPADTTLCSLIYANQTEEDILLRDELDALAKANPDRFKLWYTLDRPSGD---WKYDKGFISKDMCEAHLPEASPETIAFMCGPPAMIKFACIPNLAKIGYGKDDYISF 2503
            +QSS PEK DA L  IKG  YY+ TF+PP+E V +IDA+D+GTPDDWVPRHPELVRLTGRHPFNVEPPV R+ +HGFI+P  LHYVRNHGAVPR++  A H +RVGGAV  PLE+T+++++     E+T+PVTLVCAGNRRKEENM++QGLGFSWGPAAVS AMWTGVW++D+L H GIK   +G  HVC  GADKLPNGYYGTSIRREV MD  ADVLLAYKMNG EL+PDHGYP+R+I+PGYIGGRMIKWLTDI V + ESD+HYHYFDNRVLP QVDAE+A+AEGWWYKP YIINELNINSA+VYPRHDEV+ L  P  ++ YT+SGYAY GGG+A+TRVEVS D+G TWELAEL +PEKPRHMGRYWCW FW++KVDV RL+   EI CRAWQGQNTQP+++TWN+LGMMNN  FR K+HP R     L L FEHPT PGPQPGGWMVK                K    GK+       ++ +H +E DCWIAV+G+VYDVT FL DHPGGG+SIT+SAGQD+++EFNALHS KARAML DYYIGDL   + + A P K                    E+ LVALN ++K+ VPLIE+E L+HD+RR RF LP+  H+LGLP+GKH F+SG+W GEFVMR YTP+TGDEV GYVDLV+KVY P +RFP+GGKMSQ+LD+L +GDT+D+KGP+GEI YL    F I+  P R    + MLAGGTGITPMYQV +AVLSDPAD T   L+ ANQTE+DILLR+ELDAL  A+P R ++ YT+DR  GD   W Y  GFI+K+M  AHLP A+ + +AFMCGPP M+KFAC+PNL  +GY +  Y SF
Sbjct:   14 VQSSCPEKQDAVLARIKGSRYYEPTFMPPAEEVVDIDAKDQGTPDDWVPRHPELVRLTGRHPFNVEPPVDRLYNHGFITPASLHYVRNHGAVPRLSADA-HVLRVGGAVSTPLELTMDWLRGGALPEVTLPVTLVCAGNRRKEENMVRQGLGFSWGPAAVSTAMWTGVWLRDVLAHAGIKEHAEGAHHVCMTGADKLPNGYYGTSIRREVAMDPAADVLLAYKMNGHELTPDHGYPLRVIIPGYIGGRMIKWLTDITVGEAESDSHYHYFDNRVLPPQVDAERALAEGWWYKPQYIINELNINSAIVYPRHDEVIPLAGPGAKSEYTMSGYAYAGGGLAITRVEVSFDEGATWELAELIVPEKPRHMGRYWCWSFWQYKVDVARLLSAREITCRAWQGQNTQPKDLTWNVLGMMNNSHFRAKIHPARGASNHLGLRFEHPTQPGPQPGGWMVKSEDPAPAAVKAAAAPVKKAVEGKAVGPITEAELARHGSEADCWIAVEGRVYDVTAFLHDHPGGGDSITLSAGQDATDEFNALHSTKARAMLDDYYIGDLLSAEDAAAAPLKSAAATPVTAPASPNCAPQPREDDLVALNPKKKVKVPLIEREELTHDTRRLRFGLPSPAHRLGLPVGKHLFISGQWKGEFVMRAYTPITGDEVRGYVDLVVKVYRPCERFPEGGKMSQLLDSLALGDTLDLKGPLGEIEYLGRSAFAIRNAPPRTVRHVGMLAGGTGITPMYQVARAVLSDPADDTRLWLLAANQTEDDILLRNELDALVAAHPQRLRVHYTVDRVVGDAAAWPYSTGFITKEMIAAHLPPAADDAVAFMCGPPPMLKFACVPNLQALGYAEGSYFSF 863          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16097.1 vs. uniprot
Match: A0A5B8ME01_9CHLO (Nitrate reductase n=2 Tax=Chloropicon primus TaxID=1764295 RepID=A0A5B8ME01_9CHLO)

HSP 1 Score: 909 bits (2348), Expect = 0.000e+0
Identity = 441/811 (54.38%), Postives = 589/811 (72.63%), Query Frame = 2

Query:  128 PSEVVTEIDARDKGTPDDWVPRHPELVRLTGRHPFNVEPPVWRIKDHGFISPNELHYVRNHGAVPRITDGASHKVRVGGAVDKPLEMTLEYIKATFQEITIPVTLVCAGNRRKEENMIKQGLGFSWGPAAVSNAMWTGVWVKDILEHVGIKSFEKGGKHVCFVGADKLPNGYYGTSIRREVCMDETADVLLAYKMNGKELSPDHGYPIRLIVPGYIGGRMIKWLTDIEVTDLESDNHYHYFDNRVLPSQVDAEKAVAEGWWYKPPYIINELNINSAVVYPRHDEVVSLTSPE-TTYTVSGYAYGGGGIALTRVEVSLDDGKTWELAELSIPEKPRHMGRYWCWVFWEFKVDVLRLMKCEEIVCRAW-QGQNTQPQNITWNLLGMMNNCWFRCKVHPTRDDRGALALTFEHPTMPGPQPGGWMV------KGXXXXXXXXXXXXXXKTPSNGKSFNLKDVHKHDNEDDCWIAVDGKVYDVTDFLDDHPGGGESITISAGQDSSEEFNALHSDKARAMLQDYYIGDL-----DSSVAKP----EKVVEEEKLVALNTRQKLAVPLIEKEVLSHDSRRFRFELPTKDHKLGLPIGKHFFVSGKWNGEFVMRPYTPVTGDEVSGYVDLVIKVYTP--NDRFPKGGKMSQMLDALDIGDTIDIKGPVGEIVYLEPGQFLIKGKPRNANKLAMLAGGTGITPMYQVIKAVLSDPADTTLCSLIYANQTEEDILLRDELDALAKANPDRFKLWYTLDRPSGDWKYDKGFISKDMCEAHLPEASPETIAFMCGPPAMIKFACIPNLAKIGYGKDDYISF 2503
            P E + E+D +DKGTPD+WVPRHPEL+RLTGRHP N EPP+ R  + GF  P  LH+VRNHGA P++ +   HK+++ G VD+P+E+T++ + A    + +P+T+VCAGNRR+EENMIK+ +GFSWG +A S ++WTGV + D+L   G+++  +G  HVCFVGAD LP   YGTS++ E  +D   DVL+A++ NGK L PDHGYP+RLI+PGYIGGRM+KWLT+IEVT+ ES N+YHYFDNRVLPS VDAE+A AEGWWYKP YIIN+LNINSA+VYP+HDE++ L+  +   YT+ GYAY GGG  + R E+S DDGKTW LA+L   EKP   G+YWCW  WE +++VL L+  EEI  R+W +G N QP++ITWN++GMMNNC FR KVHP + + GALAL FEHPT+    PGGWMV      +    XXXXXXXXXXX     G +F L+++ KH+ ++  W   DGKVYD T++L+DHPGG ESI I+AGQD++EEF+A+HS KARA+L  YYIG++     D+S A+     E V E ++LVAL+  +++   L+EK VLSHD+RRFRF L + +H LGLP+G H  ++G+ +G+ VMR YTP + D+  GY DLVIKVY    + +FP GG +SQ  ++L+IGDT+++KGP+G + Y E G F + GK    N++  + GGTGITP YQ+IKA+  D  D T   LIYAN+TE+DILLR+ELD LA   P +FKL YTLD P   WK+ +GFI++DM + H+P  + + +  MCGPP MIK+AC+PNL K G+ +  Y+SF
Sbjct:   76 PEERIKEVDDKDKGTPDEWVPRHPELIRLTGRHPLNCEPPLTRNYEAGFFLPASLHFVRNHGACPQL-NWDDHKIKIHGLVDRPMEITMDQL-AMMPTVRVPITMVCAGNRRREENMIKKTIGFSWGASACSTSVWTGVRLGDLLRLCGVRTRAQGANHVCFVGADPLPKDNYGTSMKIERALDPAYDVLIAFEQNGKRLLPDHGYPVRLIIPGYIGGRMVKWLTEIEVTENESTNYYHYFDNRVLPSHVDAERATAEGWWYKPEYIINDLNINSAMVYPQHDEILKLSGSDGQKYTLKGYAYSGGGRKVIRSEISFDDGKTWNLAKLKHTEKPNAYGKYWCWCHWELEINVLDLVGIEEISLRSWDEGMNCQPKDITWNVMGMMNNCHFRVKVHPIKHE-GALALQFEHPTVAANGPGGWMVPKKSEPESAPAXXXXXXXXXXXXXXXGGATFTLEEISKHNTKESAWFVHDGKVYDATEYLEDHPGGAESILITAGQDATEEFDAIHSTKARALLDKYYIGEVGDSTSDASSARSSTSSEVVTEVKQLVALDKTKRIPFKLVEKHVLSHDTRRFRFALQSPEHILGLPVGNHMLLAGRVDGKLVMRAYTPTSSDDDVGYFDLVIKVYFKGVHPKFPDGGILSQHFESLEIGDTMEVKGPIGHVTYHEKGNFTVHGKDVKLNEVGFICGGTGITPAYQIIKAITKDKKDKTKAYLIYANKTEDDILLREELDELAAKYPKKFKLHYTLDDPPKGWKHSQGFINEDMIKRHMPSYNKKAMVMMCGPPPMIKYACVPNLEKNGFPEGSYLSF 883          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16097.1 vs. uniprot
Match: A0A1Y1IM52_KLENI (Nitrate reductase n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1IM52_KLENI)

HSP 1 Score: 875 bits (2260), Expect = 2.470e-303
Identity = 431/825 (52.24%), Postives = 555/825 (67.27%), Query Frame = 2

Query:  149 IDARDKGTPDDWVPRHPELVRLTGRHPFNVEPPVWRIKDHGFISPNELHYVRNHGAVPRITDGASHKVRVGGAVDKPLEMTLEYIKATFQEITIPVTLVCAGNRRKEENMIKQGLGFSWGPAAVSNAMWTGVWVKDILEHVGIKSFEKGGKHVCFVGADKLPNG---YYGTSIRREVCMDETADVLLAYKMNGKELSPDHGYPIRLIVPGYIGGRMIKWLTDIEVTDLESDNHYHYFDNRVLPSQVDAEKAVAEGWWYKPPYIINELNINSAVVYPRHDEVVSLTSPETTYTVSGYAYGGGGIALTRVEVSLDDGKTWELAELSIPEKPRHMGRYWCWVFWEFKVDVLRLMKCEEIVCRAWQGQ-NTQPQNITWNLLGMMNNCWFRCKVHPTRDDRGALALTFEHPTMPGPQPGGWMVK-----------GXXXXXXXXXXXXXXKTPSNGKSFNLKDVHKHDNEDDCWIAVDGKVYDVTDFLDDHPGGGESITISAGQDSSEEFNALHSDKARAMLQDYYIGDL-------DS-------------SVAKPEKVVEEEK---LVALNTRQKLAVPLIEKEVLSHDSRRFRFELPTKDHKLGLPIGKHFFVSGKWNGEFVMRPYTPVTGDEVSGYVDLVIKVYTP--NDRFPKGGKMSQMLDALDIGDTIDIKGPVGEIVYLEPGQFLIKGKPRNANKLAMLAGGTGITPMYQVIKAVLSDPADTTLCSLIYANQTEEDILLRDELDALAKANPDRFKLWYTLDRPSGDWKYDKGFISKDMCEAHLPEASPETIAFMCGPPAMIKFACIPNLAKIGYGKDDYISF 2503
            +D +D GTPDDWV RHPELVRLTGRHPFN EPP+ R+++HGF++P  LHYVRNHGAVP+  +  S ++ V G V +P  +++  + A  +  T+PVT+VCAGNRRKEENM+KQ +GF+WG A VS  +W GV + D+L+HVG     +GG +VCF G + LP G    YGTS+  EV  D   DVLLA++ NG+ L PDHG+P+R I+PGYIGGR +KWL  IEVT  ES N+YH+ DNRVLPSQVDAE+A  EGWWYKP +IINELN+ SA+  P H EV+S+ +    YT+SGYAY GGG  + RVEVS++ G++WE+AEL+    P   G++W W  W+ +VDV+ L   +E+  RAW    NTQP  I WN++GMMNNCWFR ++HP R   G + LTFEHPT PG + GGWMVK                          +  +  +S  L +V KHD+E D WI V  KVYD T FLDDHPGGGESI I+AG D +EEF A+HS KA+AML+DYYIG+L       DS             S    + + EE +   L ALN ++++   LI K+VLS D RRFRF L T  H+LGLP+GKH F+S   +G+ VMR YTP + D+  GY DLVIKVY    + +FP GG ++Q LD L IG T+D+KGPVG  VY   G F + GK ++  +++MLAGG+GITPM+QV++AVL +  D T  +L+Y NQTE DILLR+E++ALA ANPDRF++WYT+DR    W Y  G I++ M   HL  A  E+IA MCGPP MIKFACIPNL K+GY  D Y +F
Sbjct:   71 VDQKDVGTPDDWVARHPELVRLTGRHPFNCEPPLQRLREHGFMTPVSLHYVRNHGAVPK-GEWDSWRIEVSGLVGRPCSLSMADLLALPRR-TLPVTMVCAGNRRKEENMVKQTIGFNWGAAGVSTTVWGGVRLSDVLKHVGAHGKHEGGNYVCFEGEETLPGGGGSKYGTSLTMEVAADPACDVLLAFEQNGERLEPDHGFPVRTIIPGYIGGRTVKWLRKIEVTAGESQNYYHFNDNRVLPSQVDAERANREGWWYKPDFIINELNVQSAIWSPAHGEVLSIANAPAKYTISGYAYSGGGRKIIRVEVSVNGGESWEMAELAHGAPPTEYGKHWAWCLWQLEVDVMALFTTKEMAVRAWDSSMNTQPDKIAWNVMGMMNNCWFRVRIHPCRTREGGIGLTFEHPTRPGNEKGGWMVKEKEEADAAAAAAVPAAPTMKRSVSQPRMAAVQRSIPLSEVKKHDSEKDAWIVVHSKVYDATRFLDDHPGGGESILINAGTDCTEEFEAIHSAKAKAMLEDYYIGELAVTSTLGDSTPEGSLRGGSRFLSAPTLDPLTEEAEPAVLYALNPKERIPFKLIRKDVLSKDVRRFRFALQTPQHRLGLPVGKHMFLSAHIDGKLVMRAYTPTSSDDEVGYFDLVIKVYFGGVHPKFPAGGLLTQYLDGLAIGQTVDVKGPVGHFVYHGNGAFSVNGKRKHTARISMLAGGSGITPMFQVLQAVLKNKDDPTRLALLYCNQTEADILLREEMEALAAANPDRFQIWYTVDRSEPGWAYSTGHINEAMMRDHLFPADEESIACMCGPPGMIKFACIPNLEKMGYAADHYFAF 893          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16097.1 vs. uniprot
Match: Q4LEW9_PHYPA (Nitrate reductase n=3 Tax=Physcomitrium patens TaxID=3218 RepID=Q4LEW9_PHYPA)

HSP 1 Score: 874 bits (2258), Expect = 4.610e-303
Identity = 436/815 (53.50%), Postives = 560/815 (68.71%), Query Frame = 2

Query:  140 VTEIDARDKGTPDDWVPRHPELVRLTGRHPFNVEPPVWRIKDHGFISPNELHYVRNHGAVPRITDGASHKVRVGGAVDKPLEMTLEYIKATFQEITIPVTLVCAGNRRKEENMIKQGLGFSWGPAAVSNAMWTGVWVKDILEHVGIKSFEKGGKHVCFVGADKLPNG---YYGTSIRREVCMDETADVLLAYKMNGKELSPDHGYPIRLIVPGYIGGRMIKWLTDIEVTDLESDNHYHYFDNRVLPSQVDAEKAVAEGWWYKPPYIINELNINSAVVYPRHDEV--VSLTSPETTYTVSGYAYGGGGIALTRVEVSLDDGKTWELAELSIPEKPRHMGRYWCWVFWEFKVDVLRLMKCEEIVCRAWQ-GQNTQPQNITWNLLGMMNNCWFRCKVHPTRDDRGALALTFEHPTMPGPQPGGWMVKGXXXXXXXXXXXXXXKT-----PSNGKSFNLKDVHKHDNEDDCWIAVDGKVYDVTDFLDDHPGGGESITISAGQDSSEEFNALHSDKARAMLQDYYIGDLDSSVAK-------------PEKVVEEEKLVALNTRQKLAVPLIEKEVLSHDSRRFRFELPTKDHKLGLPIGKHFFVSGKWNGEFVMRPYTPVTGDEVSGYVDLVIKVYTPN--DRFPKGGKMSQMLDALDIGDTIDIKGPVGEIVYLEPGQFLIKGKPRNANKLAMLAGGTGITPMYQVIKAVLSDPADTTLCSLIYANQTEEDILLRDELDALAKANPDRFKLWYTLDRPSGD-WKYDKGFISKDMCEAHLPEASPETIAFMCGPPAMIKFACIPNLAKIGYGKDDYISF 2503
            +T++D RDKGT DDW+PRHPELVRLTGRHPFN EPP+  + + GF++P  LHYVRNHGAVPR +     KV V G V +P+ +T+  I   F+   +PVTLVCAGNRRKEENM+KQ +GF+WG AAVS ++W GV + D+L H G+ S +KG  +VCF GA+ LP G    YGTSI  E  MDE  DV+LAY+ NG+ L PDHG+PIRLI+PGYIGGRM+KWL  IEVT  ES N+YH+ DNRVLPS VDAE A AEGWWY+P YIIN+LNINSA+  P H E   V+ +S +  Y V GYAY GGG  +TRVEVSLDDGK+W L ++  PE+P   G+YWCW FW+  VDV+ L+KC+EIV RAW  G NTQPQ++ WN++GMMNNCWFR KV  ++ +   + L FEHPT PG Q GGWM K               +T     P   +   + +V KH  E+ CWI V  KVYD T FL+DHPGG +SI I+ G DS+EEF+A+HS KA+ ML++YYIGDL +S A+             P  +    + VAL+ ++++A  LIE+EVLSHD RR RF L +++H LGLP+GKH  +S   NG+  MR YTP + D+  GY++LVIKVY  +   +FP GG  SQ LD L +GDTI+ KGPVG IVY   GQFLI GKP+   ++AMLAGGTGITPMYQVI+A++SDP D T   L+Y+N+TEEDI+LR ELDA  + + +   + YTL   + + W++ KG IS+ M + H+PE   E++A +CGP  +I+ ACIP L K  Y K   + F
Sbjct:   80 ITDVDERDKGTSDDWIPRHPELVRLTGRHPFNCEPPLSTLMEAGFLTPTSLHYVRNHGAVPRAS-WDDWKVDVSGLVKRPMSLTMNDI-LRFRARELPVTLVCAGNRRKEENMVKQSIGFNWGAAAVSTSIWRGVLLCDVLRHCGVVSRKKGALYVCFEGAEILPGGGGSTYGTSITIETAMDEAQDVMLAYQQNGQYLEPDHGFPIRLIIPGYIGGRMVKWLNKIEVTSRESGNYYHFHDNRVLPSHVDAETAKAEGWWYRPDYIINQLNINSAISSPAHGETLPVNFSSLQEPYIVKGYAYSGGGRKVTRVEVSLDDGKSWTLCDVKHPEEPTRYGKYWCWCFWQIGVDVMDLLKCKEIVVRAWDAGMNTQPQHLIWNVMGMMNNCWFRVKVGISKFEDSGIHLAFEHPTRPGNQVGGWMTKSEVPQAAAPVLSKVVETKVTQLPVAVRQIPVSEVRKHQTEESCWIIVRNKVYDCTPFLNDHPGGADSILINGGMDSTEEFDAIHSAKAQTMLEEYYIGDLSASTAEVVDVAPKTEAEAIPTALSASGRPVALSLKERIAFRLIEREVLSHDVRRLRFALQSENHVLGLPVGKHVLLSASINGKLCMRAYTPTSNDDDVGYLELVIKVYFKDVHPKFPMGGMFSQHLDTLRVGDTIEAKGPVGHIVYEGKGQFLINGKPKFVRRVAMLAGGTGITPMYQVIRAIVSDPEDKTQVWLLYSNRTEEDIMLRKELDAWQEKH-NNLVVRYTLTGTASEGWRFSKGRISEAMVKEHIPEGGEESLALLCGPQELIQEACIPCLLKHKYEKSACLEF 891          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16097.1 vs. uniprot
Match: G8IRI1_HETAK (NR2-2/2HbN n=7 Tax=Sar TaxID=2698737 RepID=G8IRI1_HETAK)

HSP 1 Score: 868 bits (2243), Expect = 3.340e-300
Identity = 434/924 (46.97%), Postives = 581/924 (62.88%), Query Frame = 2

Query:  119 FVPPSEVVTEIDARDKGTPDDWVPRHPELVRLTGRHPFNVEPPVWRIKDHGFISPNELHYVRNHGAVPRITDGASHKVRVGGAVDKPLEMTL-EYIKAT-FQEITIPVTLVCAGNRRKEENMIKQGLGFSWGPAAVSNAMWTGVWVKDILEHVGIKSFEKGGKHVCFVGADKLPNGYYGTSIRREVCMDETADVLLAYKMNGKELSPDHGYPIRLIVPGYIGGRMIKWLTDIEVTDLESDNHYHYFDNRVLPSQVDAEKAVAEGWWYKPPYIINELNINSAVVYPRHDEVVSLT-SPETTYTVSGYAYGGGGIALTRVEVSLDDGKTWELAELSIPEKPRHMGRYWCWVFWEFKVDVLRLMKCEEIVCRAWQ-GQNTQPQNITWNLLGMMNNCWFRCKVHPTRDDRGALALTFEHPTMPGPQPGGWMVKGXXXXXXXXXXXXXXKTPSNGKSFNLKDVHKHDNEDDCWIAVDGKVYDVTDFLDDHPGGGESITISAGQDSSEEFNALHSDKARAMLQDYYIGDLDSSVAKP----------------------------EKVVEEEKLV---------------------------------------------------------------------------------------------ALNTRQKLAVPLIEKEVLSHDSRRFRFELPTKDHKLGLPIGKHFFVSGKWNGEFVMRPYTPVTGDEVSGYVDLVIKVYTPND--RFPKGGKMSQMLDALDIGDTIDIKGPVGEIVYLEPGQFLIKGKPRNANKLAMLAGGTGITPMYQVIKAVLSDPADTTLCSLIYANQTEEDILLRDELDALAKANPDRFKLWYTLDRPSGD--WKYDKGFISKDMCEAHLPEASPETIAFMCGPPAMIKFACIPNLAKIGYGKDDYISF 2503
            +  P   + E+D RD+ TPDDW+PRHP+LVRLTGRHPFN EPPV  +  HGFI+P  LHYVRNHGA P + D  SH+VR+ G VDKP+E+++ ++   T F++++IPVTLVCAGNRRKE+N +KQG+GF+WGPAAVS ++WTGV V+D+LE+ G+KS ++G  HVCFVGAD LP GYYGTSI R   MD  +DV+L ++ NG+ L+PDHGYPIRLI+PGYIGGRMIKWLTDI VT++ESDNHYHY DNRVLP Q+DA+ A A+GWWYKP YIIN+LNINSA+  P HDEVV++    + TY   GYAY GGG  +TRVE+S D+G+TWEL  L+ PE+P   G++WCW FWE++V ++R+++ ++++ RAW  G NTQP N TWN++GMMNNC F+ ++H   +  G L+L FEHPT PG  PGGWMV                +  +  K F  ++V KH   DD W   DGKVYD T F+DDHP G +SI ++AG+D++EEF++LHS+KA+ ML DYYIG+L ++ A                              EKV+++  L                                                                                              ALN ++K+A  L+E+E ++H+ RR RF L + +H LGLP+G+H FVS K +G   MR YTP+TGDEV GY DL+IKVY  N+  +FP+GGKMSQ L++L IG TID++GP+G I Y   G F I GK      + M+AGGTGITPM+QV+ AVL D AD+T  +LI+AN TE+DILL++EL+ +   N ++ ++++T+  P     W    GFI+++M E     A  + I F+CGPP MI FAC+P L  +GY K+    F
Sbjct:   12 YTCPRVDIREVDPRDEKTPDDWIPRHPDLVRLTGRHPFNCEPPVMDLMSHGFITPTSLHYVRNHGAAPNL-DWDSHRVRISGLVDKPMELSMADFTDPTKFEQVSIPVTLVCAGNRRKEQNSVKQGIGFNWGPAAVSTSVWTGVRVRDVLEYCGLKSQDEGANHVCFVGADPLPGGYYGTSIIRHAAMDPASDVMLCWEQNGERLTPDHGYPIRLIIPGYIGGRMIKWLTDISVTEVESDNHYHYHDNRVLPPQIDADTAKADGWWYKPEYIINDLNINSAITSPAHDEVVTIIPGQKATYACKGYAYSGGGRKVTRVELSFDEGETWELTTLTHPERPTRAGKHWCWCFWEYEVPIMRMLRAKQMMVRAWDTGLNTQPMNFTWNVMGMMNNCTFKVRIHDASEGNG-LSLKFEHPTQPGVLPGGWMVP-KVEVQAAVQVEKKVEAKAGVKYFTEEEVAKHTERDDAWFIYDGKVYDATPFMDDHPSGADSILLTAGEDATEEFDSLHSEKAKKMLDDYYIGELGTAPAASAPPPPAVSLFEKLGGGEAVNAVVNKFYDEKVLKDTSLSPIFDGKDVESLKMHQRMFLQWVLGGENGYTGRSMKEAHAGLGITEAHWNTVCGHLVGTLQELGVSAADIDTVVSKVAPLKDDVVGTSALNRPKALNKKKKMAFALVEREEITHNVRRLRFALQSPEHVLGLPVGQHMFVSAKIDGALCMRAYTPLTGDEVQGYFDLLIKVYYANEHPKFPEGGKMSQHLNSLTIGQTIDVRGPLGHIDYKGKGLFDIDGKEIQCRDILMMAGGTGITPMWQVMSAVLRDEADSTKLNLIFANNTEDDILLQEELNDMDSEN-EQCQVYHTIATPKNPETWSQGVGFITQEMVEQQFGPARDDAIVFLCGPPPMINFACLPALEALGYKKEQIFQF 931          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16097.1 vs. uniprot
Match: A9SHD3_PHYPA (Nitrate reductase n=2 Tax=Physcomitrium patens TaxID=3218 RepID=A9SHD3_PHYPA)

HSP 1 Score: 867 bits (2239), Expect = 3.520e-300
Identity = 434/811 (53.51%), Postives = 553/811 (68.19%), Query Frame = 2

Query:  140 VTEIDARDKGTPDDWVPRHPELVRLTGRHPFNVEPPVWRIKDHGFISPNELHYVRNHGAVPRITDGASHKVRVGGAVDKPLEMTLEYIKATFQEITIPVTLVCAGNRRKEENMIKQGLGFSWGPAAVSNAMWTGVWVKDILEHVGIKSFEKGGKHVCFVGADKLPNG---YYGTSIRREVCMDETADVLLAYKMNGKELSPDHGYPIRLIVPGYIGGRMIKWLTDIEVTDLESDNHYHYFDNRVLPSQVDAEKAVAEGWWYKPPYIINELNINSAVVYPRHDEV--VSLTSPETTYTVSGYAYGGGGIALTRVEVSLDDGKTWELAELSIPEKPRHMGRYWCWVFWEFKVDVLRLMKCEEIVCRAWQG-QNTQPQNITWNLLGMMNNCWFRCKVHPTRDDRGALALTFEHPTMPGPQPGGWMVKGXXXXXXXXXXXXXX-KTPSNGKSFNLKDVHKHDNEDDCWIAVDGKVYDVTDFLDDHPGGGESITISAGQDSSEEFNALHSDKARAMLQDYYIGDLDSS-------VAKPEK------VVEEEKLVALNTRQKLAVPLIEKEVLSHDSRRFRFELPTKDHKLGLPIGKHFFVSGKWNGEFVMRPYTPVTGDEVSGYVDLVIKVYTPN--DRFPKGGKMSQMLDALDIGDTIDIKGPVGEIVYLEPGQFLIKGKPRNANKLAMLAGGTGITPMYQVIKAVLSDPADTTLCSLIYANQTEEDILLRDELDALAKANPDRFKLWYTLDRPSG-DWKYDKGFISKDMCEAHLPEASPETIAFMCGPPAMIKFACIPNLAKIGYGKDDYISF 2503
            +T++D RDKGT DDW+PRHPELVRLTGRHPFN EPP+  + + GF++P  LHYVRNHGAVPR +     K+ + G V +P   T+  I   F    +PVTLVCAGNRRKEEN++KQ +GF+WG AAVS ++W GV + DIL H G+ S +KG  +VCF GA+ LP G    YGTSI  +  MD+  DVLLAY+ NG+ L PDHG+PIRLI+PGYIGGRM+KWL  IEVT  ES N+YH+ DNRVLPS VDAE A AEGWWY+P YIIN+LNINSA+  P H E   V+ ++ + +Y V GYAY GGG  +TRVEVSL+DGK+W L +L  PE+P   G+YWCW FW+  VDVL L+KC+EIV RAW    NTQPQ++ WN++GMMNNCWFR K+  T+ +   + LTF+HPT PG Q GGWMVK                K  +  +   L +V KH   D CWI V  KVYD T FLDDHPGG +SI I+ G DS+EEF+A+HS KA+AML++YYIGDL +S       + K EK      V    + VALN R++L   LIEKE LSHD RR RF L +++H LGLP+GKH  +S   NG+F MR YTP++ D+  GY +LVIKVY  N   +FP GG  SQ LD+L IGDTID+KGPVG IVY   G FLI GK +   K++MLAGGTGITPMYQVI+A++SDP D T   L+Y+N+ E DI+LR ELD   + + +  K+ +TL   +  DW + KG I + M + H+PE    ++A +CGP  +I+ AC+P+L    + K   + F
Sbjct:   85 ITKVDERDKGTSDDWIPRHPELVRLTGRHPFNCEPPLATLMEAGFLTPTSLHYVRNHGAVPRAS-WDDWKIEISGLVKRPTTFTMNDI-LRFPARELPVTLVCAGNRRKEENIVKQSIGFNWGAAAVSTSIWRGVRLCDILRHCGVVSRKKGALYVCFEGAEILPGGGGSTYGTSITIDTAMDKAQDVLLAYQQNGQCLEPDHGFPIRLIIPGYIGGRMVKWLNKIEVTTQESGNYYHFHDNRVLPSHVDAETAKAEGWWYRPDYIINQLNINSAITSPAHGEALPVNFSTLQESYIVKGYAYSGGGRKVTRVEVSLNDGKSWILCDLKHPEEPTRYGKYWCWCFWQINVDVLDLLKCKEIVVRAWDAAMNTQPQHLIWNVMGMMNNCWFRVKIGITKSEDNGIHLTFDHPTQPGNQTGGWMVKSEAQKPAEPLIKIIETKVAAPARQIKLSEVRKHQKADSCWIIVRNKVYDCTPFLDDHPGGADSILINGGTDSTEEFDAIHSAKAQAMLEEYYIGDLVASKDDLEVELVKDEKEDIAKLVSSSGRPVALNPRERLPFRLIEKETLSHDVRRLRFALQSENHVLGLPVGKHILLSATINGKFCMRAYTPISNDDDVGYFELVIKVYYKNAHSKFPMGGLFSQYLDSLRIGDTIDVKGPVGHIVYEGKGHFLINGKSKFVKKVSMLAGGTGITPMYQVIRAIVSDPEDQTQICLLYSNRNEGDIMLRKELDTWQQQH-ENLKVHFTLTGAAPEDWAFSKGRICEAMIKEHIPEGCEASLALLCGPQELIQSACLPSLLNHKFDKSICLEF 892          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16097.1 vs. uniprot
Match: A0A8J9RHI9_9CHLO (Nitrate reductase [NADH] n=1 Tax=Coccomyxa sp. Obi TaxID=2315456 RepID=A0A8J9RHI9_9CHLO)

HSP 1 Score: 843 bits (2177), Expect = 2.820e-291
Identity = 414/812 (50.99%), Postives = 544/812 (67.00%), Query Frame = 2

Query:  134 EVVTEIDARDKGTPDDWVPRHPELVRLTGRHPFNVEPPVWRIKDHGFISPNELHYVRNHGAVPRITDGASHKVRVGGAVDKPLEMTLEYIKATFQEITIPVTLVCAGNRRKEENMIKQGLGFSWGPAAVSNAMWTGVWVKDILEHVGIKSFEKGGKHVCFVGA-DKLP---NGYYGTSIRREVCMDETADVLLAYKMNGKELSPDHGYPIRLIVPGYIGGRMIKWLTDIEVTDLESDNHYHYFDNRVLPSQVDAEKAVAEGWWYKPPYIINELNINSAVVYPRHDEVVSLTSPETTYTVSGYAYGGGGIALTRVEVSLDDGKTWELAELSIPEKPRHMGRYWCWVFWEFKVDVLRLMKCEEIVCRAW-QGQNTQPQNITWNLLGMMNNCWFRCKVHPTRDDRGALALTFEHPTMPGPQPGGWMVKGXXXXXXXXXXXXXXKT---PSNG--KSFNLKDVHKHDNEDDCWIAVDGKVYDVTDFLDDHPGGGESITISAGQDSSEEFNALHSDKARAMLQDYYIGDLDSSVAKPE--------KVVEEEKLVALNTRQKLAVPLIEKEVLSHDSRRFRFELPTKDHKLGLPIGKHFFVSGKWNGEFVMRPYTPVTGDEVSGYVDLVIKVYTPND--RFPKGGKMSQMLDALDIGDTIDIKGPVGEIVYLEPGQFLIKGKPRNANKLAMLAGGTGITPMYQVIKAVLSDPADTTLCSLIYANQTEEDILLRDELDALAKANPDRFKLWYTLDR-PSG-DWKYDKGFISKDMCEAHLPEASPETIAFMCGPPAMIKFACIPNLAKIGYGKDDYISF 2503
            E VTEID +D  TPD WVPRHP+L+RLTGRHP NVEP    +  +GFI+P  LHYVRNHG VP+I   + HKV + G V++P   T++ + +TF+ + +  TL CAGNRRKE+NM+ + +GF+WGPA     +WTGV + D+L H G+KS E+G  +VCF G  ++LP   +G YGTS+ R   +D  +D++LA+K NG+ L+PDHGYP+R+I+PGYIGGRM+KWL +I VT+ ESDN YH+ DNRVLPS V    A  EGWWYKP +IIN++NINSA+  P HDEVV L S    + + GYAY G G  + R EV++D  KTW L ++     P   G++W WV W  K+ ++ L++ EE+VCRAW +  NTQP + TWNL+GM+NNC +R KVHP     G LAL FEHPT+ G   GGWM +               +    P  G  K+F +++V KHD  +  W    G+VYD T FL +HPGG +SI + AG D++EEFNA+HS+KA+  L  Y IG L SS   PE         V E+   VALN +++L   L+EK+ LSH+ RRFRF LP+  HK GLP+GKH F+    NGE VMR YTP + D+  GY DLVIKVY  N+  RFP GGKMSQ L++L IG  +++KGP+G + YL   ++ + G+ R    L+M+AGGTGITP YQVIKAVL D  D T  SL+YANQ+ +DILL DEL  +AK +P RF++WYT+DR P G +W Y  GF+++DM   HL  A PET A +CGPP MIKFAC+PNL K+GY  +D I F
Sbjct:   55 EPVTEIDPKDAATPDAWVPRHPDLIRLTGRHPLNVEPTPTALMKYGFITPASLHYVRNHGPVPKI-QWSQHKVEINGLVERPTSFTMDQLLSTFEHVDVLCTLTCAGNRRKEQNMVAKTIGFNWGPAGTGTGVWTGVRLADVLRHCGMKSAEQGANYVCFRGPKNELPQGKDGSYGTSVERYKALDPASDIILAFKQNGRLLTPDHGYPLRIIIPGYIGGRMVKWLEEITVTETESDNFYHFHDNRVLPSHVTEVLAKKEGWWYKPDFIINDININSAITSPAHDEVVKLDSRNAYFNLCGYAYSGAGRKIIRCEVTVDGSKTWRLGDIVRAGPPNAYGKHWAWVHWSLKIPLVDLLRAEEVVCRAWDEAMNTQPDSFTWNLMGMLNNCHYRVKVHPVELPEGGLALQFEHPTIAGVALGGWMNRADEAAKEVAEVPKLAEAQAAPKGGDVKTFTMEEVEKHDTRESAWFVHAGQVYDGTPFLKEHPGGADSILLVAGTDATEEFNAIHSEKAKKQLLQYVIGRLASSTH-PEVGKAPLVPAVPEQTAPVALNPKKRLKFRLVEKQELSHNVRRFRFALPSPQHKFGLPVGKHVFLYANVNGELVMRAYTPTSSDDELGYFDLVIKVYWKNEHPRFPDGGKMSQHLESLPIGGEMEVKGPLGHVHYLGRNKYTLDGEMRTGKHLSMIAGGTGITPCYQVIKAVLKDAEDDTQLSLLYANQSPDDILLFDELQEMAK-DP-RFRVWYTVDRVPEGMEWAYSTGFVNEDMVREHLFPAGPETAALLCGPPPMIKFACMPNLEKLGYKAEDCICF 862          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16097.1 vs. uniprot
Match: UPI0010FB6302 (nitrate reductase [NADH] 2 n=1 Tax=Cajanus cajan TaxID=3821 RepID=UPI0010FB6302)

HSP 1 Score: 839 bits (2167), Expect = 2.080e-289
Identity = 417/829 (50.30%), Postives = 543/829 (65.50%), Query Frame = 2

Query:  104 YYKDTFVPPSEVVTE---IDARDKGTPDDWVPRHPELVRLTGRHPFNVEPPVWRIKDHGFISPNELHYVRNHGAVPRITDGASHKVRVGGAVDKPLEMTLEYIKATFQEITIPVTLVCAGNRRKEENMIKQGLGFSWGPAAVSNAMWTGVWVKDILEHVGIKSFEKGGKHVCFVGADKLPNG---YYGTSIRREVCMDETADVLLAYKMNGKELSPDHGYPIRLIVPGYIGGRMIKWLTDIEVTDLESDNHYHYFDNRVLPSQVDAEKAVAEGWWYKPPYIINELNINSAVVYPRHDEVVSLT--SPETTYTVSGYAYGGGGIALTRVEVSLDDGKTWELAELSIPEKPRHMGRYWCWVFWEFKVDVLRLMKCEEIVCRAW-QGQNTQPQNITWNLLGMMNNCWFRCKVHPTRDDRGALALTFEHPTMPGPQPGGWMVKGXXXXXXXXXXXXXXKTPSN------GKSFNLKDVHKHDNEDDCWIAVDGKVYDVTDFLDDHPGGGESITISAGQDSSEEFNALHSDKARAMLQDYYIGDLDS----SVAKPEKVVE--------------EEKLVALNTRQKLAVPLIEKEVLSHDSRRFRFELPTKDHKLGLPIGKHFFVSGKWNGEFVMRPYTPVTGDEVSGYVDLVIKVYTP--NDRFPKGGKMSQMLDALDIGDTIDIKGPVGEIVYLEPGQFLIKGKPRNANKLAMLAGGTGITPMYQVIKAVLSDPADTTLCSLIYANQTEEDILLRDELDALAKANPDRFKLWYTLDRPSGDWKYDKGFISKDMCEAHLPEAS--PETIAFMCGPPAMIKFACIPNLAKIGY 2479
            Y KD  +  +   TE   +D RD+GT D W+ R+  +VRLTG+HPFN EPP+ R+  HGFI+P  LHYVRNHG VP+        V + G V +P   T++ +   F     P TLVCAGNRRKE+NM+KQ +GF+WG   VS ++W GV ++ +L   GI +  KG  HVCF GA+ LP G    YGTS+ REV MD + D++LAY  NG+ L+PDHG+PIR+I+PG+IGGRM+KWL  I VT +ES+NHYHY DNRVLPS VDAE A  EGWWYKP YIINELNINS +  P H+E++ +   + +  Y + GYAY GGG  +TRVEV+LD G+TW +  L  PEKP   G+YWCW FW  +V+VL L+  +EI  RAW +G NTQP+N+ WN++GMMNNCWFR K +  +  +G + L FEHPT PG QPGGWM K               K+ S        K F++ +V KH N D  WI V G VYD + FL DHPGG +SI I+AG D +EEF+A+HSDKA+ ML+DY IG+L +    S + P   V                ++ VALN R+K+   L+ K V+S D R FR  LP+++  LGLP+GKH FV    +G+  MR YTP +G E  G+++L+IKVY    + +FP GG MSQ LD+L +G T+D+KGP+G I Y   G FL+ GK R A KLAMLAGGTGITP+YQV +A+L DP D T   ++YAN+TE+DILLR+ELDA A+ + D FKLWY ++ P   W Y  G I++ +   HLP+ S   ET+A  CGPP MI+FA  PNL K+GY
Sbjct:   51 YLKD-LIQKANAETEASVMDPRDEGTADQWIARNASMVRLTGKHPFNSEPPLTRLMHHGFITPVPLHYVRNHGPVPKAR-WEDWTVEITGLVKRPTRFTVDRLVHDFPSREFPATLVCAGNRRKEQNMVKQSVGFNWGSGGVSTSVWRGVPLRHVLRRCGIYARAKGALHVCFEGAEDLPGGGGSKYGTSVTREVAMDPSRDIILAYMQNGEPLAPDHGFPIRMIIPGFIGGRMVKWLKRIVVTQVESENHYHYKDNRVLPSHVDAELATDEGWWYKPEYIINELNINSVITTPCHEEILPINQWTTQRPYVLRGYAYSGGGRKVTRVEVTLDGGETWHVCALDRPEKPNKYGKYWCWCFWSLEVEVLDLLGTKEIAVRAWDEGLNTQPENLIWNVMGMMNNCWFRVKTNVCKPHKGEIGLVFEHPTQPGNQPGGWMAKERHLEKSQEPKPTLKKSVSTPFMNTASKMFSISEVKKHSNPDSAWIIVHGHVYDCSRFLKDHPGGTDSILINAGSDCTEEFDAIHSDKAKKMLEDYRIGELITTGYTSDSSPNSSVHGNSEFTFLAPIKEAPQRSVALNPREKIPCKLVSKTVISDDVRLFRLALPSEEQVLGLPVGKHIFVCASMDGKLCMRAYTPTSGVEEVGFLELLIKVYFKGVHPKFPNGGLMSQHLDSLPLGSTVDVKGPLGHIEYTGRGNFLVHGKARFAKKLAMLAGGTGITPVYQVAQAILKDPEDRTQMHVVYANRTEDDILLREELDAWAREHCDSFKLWYVVESPREGWAYSVGLITETIMREHLPQPSGEEETLALTCGPPPMIQFAVQPNLEKMGY 877          

BLAST of mRNA_E-siliculosus-1a_M_contig8.16097.1 vs. uniprot
Match: B9H7A1_POPTR (Nitrate reductase n=5 Tax=Saliceae TaxID=238069 RepID=B9H7A1_POPTR)

HSP 1 Score: 839 bits (2168), Expect = 2.290e-289
Identity = 415/817 (50.80%), Postives = 555/817 (67.93%), Query Frame = 2

Query:  149 IDARDKGTPDDWVPRHPELVRLTGRHPFNVEPPVWRIKDHGFISPNELHYVRNHGAVPRITDGASHKVRVGGAVDKPLEMTLEYIKATFQEITIPVTLVCAGNRRKEENMIKQGLGFSWGPAAVSNAMWTGVWVKDILEHVGIKSFEKGGKHVCFVGADKLPNG---YYGTSIRREVCMDETADVLLAYKMNGKELSPDHGYPIRLIVPGYIGGRMIKWLTDIEVTDLESDNHYHYFDNRVLPSQVDAEKAVAEGWWYKPPYIINELNINSAVVYPRHDEVVSLTS--PETTYTVSGYAYGGGGIALTRVEVSLDDGKTWELAELSIPEKPRHMGRYWCWVFWEFKVDVLRLMKCEEIVCRAW-QGQNTQPQNITWNLLGMMNNCWFRCKVHPTRDDRGALALTFEHPTMPGPQPGGWMVKGXXXXXXXXXXXXXXKTPS------NGKSFNLKDVHKHDNEDDCWIAVDGKVYDVTDFLDDHPGGGESITISAGQDSSEEFNALHSDKARAMLQDYYIGDLDSSVA----------------KPEKVVEEEKLVALNTRQKLAVPLIEKEVLSHDSRRFRFELPTKDHKLGLPIGKHFFVSGKWNGEFVMRPYTPVTGDEVSGYVDLVIKVYTP--NDRFPKGGKMSQMLDALDIGDTIDIKGPVGEIVYLEPGQFLIKGKPRNANKLAMLAGGTGITPMYQVIKAVLSDPADTTLCSLIYANQTEEDILLRDELDALAKANPDRFKLWYTLDRPSGD-WKYDKGFISKDMCEAHLPEASPETIAFMCGPPAMIKFACIPNLAKIGYG-KDDYISF 2503
            ID+RD+GT D+W+ R+P +VRLTG+HPFN EPP+ R+  HGFI+P  LHYVRNHG VP+ T      V V G V +P   T++ +   F    +PVTLVCAGNRRKE+NM+KQ +GF+WG A VS ++W GV +  +L+  GI S +KG  +VCF GA+ LP G    YGTSI++E  MD + D++LAY  NG+ L+PDHG+P+R+I+PG+IGGRM+KWL  I VT +ESDN+YHY DNRVLPS VDAE A AE WWYKP YIINELNINS +  P H+E++ + S   ++ YT+ GYAY GGG  +TRVEV+LD G+TW++  L   EKP   G+YWCW FW  +V+VL L+  +EI  RAW +  NTQP+ + WN++GMMNNCWFR K +  +  +G + + FEHPT+PG Q GGWM K               K+ S      + K+F++ +V KH++ D  WI V G VYD T FL DHPGG +SI I+AG D +EEF+A+HSDKA+ ML+D+ IG+L SS A                 P K +   + VAL  R+K+   LI+KE+LSHD R FRF LP++D  LGLP+GKH F+    N +  MR YTP +  +V GY DLVIKVY    + +FP GG+MSQ L++L +G  ID+KGP+G I Y+  G+FL++ KP+ A KL MLAGGTGITP+YQ+I+A+L DP D T   L+YAN+TE+DILLRDELD+ AK + +R K+W+ +     + W+Y  GF+++ +   H+PE S +T+A  CGPP MI+FA  PNL K+ Y  KD  + F
Sbjct:   85 IDSRDEGTADNWIERNPSMVRLTGKHPFNSEPPLARLMHHGFITPVPLHYVRNHGPVPKAT-WQDWTVEVCGLVKRPARFTMDKLVNEFPARELPVTLVCAGNRRKEQNMVKQTIGFNWGSAGVSTSVWRGVPLHLLLKKCGIYSRKKGALNVCFEGAEDLPGGGGSKYGTSIKKEFAMDPSRDIILAYMQNGEPLAPDHGFPVRMIIPGFIGGRMVKWLKRIIVTSVESDNYYHYMDNRVLPSHVDAELANAEAWWYKPEYIINELNINSVITTPSHEEILPINSWTTQSPYTLRGYAYSGGGKKVTRVEVTLDGGETWQVCSLDHEEKPNKYGKYWCWCFWSLEVEVLELLGAKEIAVRAWDETLNTQPEKLNWNIMGMMNNCWFRVKTNVCKRHKGEIGIIFEHPTVPGNQSGGWMAKQRHLEKSLENIQALKKSVSTPFMNTSSKTFSMAEVKKHNSADSAWIIVHGHVYDCTRFLKDHPGGTDSILINAGTDCTEEFDAIHSDKAKKMLEDHRIGELVSSSAYTSDSNASSPNNSVHLAPIKEIASIRNVALVPREKIPCKLIKKEILSHDVRLFRFALPSEDQVLGLPVGKHIFLCATVNDKLCMRAYTPASTVDVVGYFDLVIKVYFKGVHPKFPNGGQMSQYLNSLSLGSVIDVKGPLGHIEYVGRGKFLVRDKPKFAKKLTMLAGGTGITPIYQLIQAILKDPEDDTEMYLVYANRTEDDILLRDELDSWAKEH-ERLKVWHVIQESIKEGWQYSVGFVTESILREHVPEGSDDTLALACGPPPMIQFAVQPNLEKMNYDIKDSLLVF 899