prot_E-siliculosus-1a_F_contig1117.784.1 (polypeptide) Ectocarpus siliculosus Ec863f_EcPH12_90f female

BLAST of mRNA_E-siliculosus-1a_F_contig1117.784.1 vs. uniprot
Match: A0A6H5K8I7_9PHAE (PFK domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K8I7_9PHAE)

HSP 1 Score: 885 bits (2287), Expect = 0.000e+0
Identity = 435/444 (97.97%), Postives = 441/444 (99.32%), Query Frame = 0

Query:    8 MCEVPNLNVKFPSSLAPNREITVNTQGSPTCYNKLLGPRDCIIYGECQHVRDKNKPPTAKAFLRAGPRPELHFNPTEVRGAIVTCGGLCPGLNSVVHHLVETMLLTYKADKVFGIRGGFAGFYDEATPAVELTSEGVEFLQHQPGSVLGSSRGGFDLDKIIKFLVDNKVNQLYIIGGDGTHRGALRIASECMQRGLNISVTGIPKTIDNDLDIIDRSFGFLTAVESAQAAIQAATTEAKCNLPNGIGVVKLMGRSAGYIAAYATMASGDVDLCLVPESPIVLEGPSGCIPHLMKRVEEKGYAVVVVAEGAGEELLGESTAKDASGNKRLPAIGKFMKGAIEDAYHAEDKICNVKYIDPSYMIRSVAATAFDQIYCMQLAQNAVHGAMAGYTAFSAGVVNNRTVYIPITELVENSPRCLNMHGRTWERVLTITRQPNTIESRAGK 451
            MCEVPNLNVKFPSSLAPNREITVNTQGSPTCYNKLLGPRDCIIYGECQHVRDKNKPPTAKAFLRAGPRPELHFNPTEVRGAIVTCGGLCPGLNSVVHHLVETML+TYKADKVFGIRGGFAGFYDEATPAVELTSEGVEFLQHQPGSVLGSSRGGFDLDKIIKFLVDNKVNQLYIIGGDGTHRGALRIA+ECMQRGLNISVTGIPKTIDNDLDIIDRSFGFLTAVESAQAAIQ ATTEAKCNLPNGIGVVKLMGRSAGYIAAYATMASGDVDLCLVPESPIVLEGP+GCIPHLMKRVEEKGYAVVVVAEGAGEELLGE+TAKDASGNKRLPAIGKFMKGAIEDA+ AEDKICNVKYIDPSYMIRSVAATAFDQIYCMQLAQNAVHGAMAGYTAFSAGVVNNRTV+IPITELVENSPRCLNMHGRTWERVLTITRQPNTIESRA K
Sbjct:    1 MCEVPNLNVKFPSSLAPNREITVNTQGSPTCYNKLLGPRDCIIYGECQHVRDKNKPPTAKAFLRAGPRPELHFNPTEVRGAIVTCGGLCPGLNSVVHHLVETMLVTYKADKVFGIRGGFAGFYDEATPAVELTSEGVEFLQHQPGSVLGSSRGGFDLDKIIKFLVDNKVNQLYIIGGDGTHRGALRIATECMQRGLNISVTGIPKTIDNDLDIIDRSFGFLTAVESAQAAIQVATTEAKCNLPNGIGVVKLMGRSAGYIAAYATMASGDVDLCLVPESPIVLEGPTGCIPHLMKRVEEKGYAVVVVAEGAGEELLGENTAKDASGNKRLPAIGKFMKGAIEDAFQAEDKICNVKYIDPSYMIRSVAATAFDQIYCMQLAQNAVHGAMAGYTAFSAGVVNNRTVFIPITELVENSPRCLNMHGRTWERVLTITRQPNTIESRACK 444          

BLAST of mRNA_E-siliculosus-1a_F_contig1117.784.1 vs. uniprot
Match: A0A6H5KDC4_9PHAE (PFK domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KDC4_9PHAE)

HSP 1 Score: 617 bits (1591), Expect = 2.610e-215
Identity = 297/447 (66.44%), Postives = 363/447 (81.21%), Query Frame = 0

Query:    3 LDKIIMCEVPNLNVKFPSSLAPNREITVNTQGSPTCYNKLLGPRDCIIYGECQHVRDKNKPPTAKAFLRAGPRPELHFNPTEVRGAIVTCGGLCPGLNSVVHHLVETMLLTYKADKVFGIRGGFAGFYDEATPAVELTSEGVEFLQHQPGSVLGSSRGGFDLDKIIKFLVDNKVNQLYIIGGDGTHRGALRIASECMQRGLNISVTGIPKTIDNDLDIIDRSFGFLTAVESAQAAIQAATTEAKCNLPNGIGVVKLMGRSAGYIAAYATMASGDVDLCLVPESPIVLEGPSGCIPHLMKRVEEKGYAVVVVAEGAGEELLGESTAKDASGNKRLPAIGKFMKGAIEDAYHAEDKICNVKYIDPSYMIRSVAATAFDQIYCMQLAQNAVHGAMAGYTAFSAGVVNNRTVYIPITELVENSPRCLNMHGRTWERVLTITRQPNTIESRA 449
            +++I+   VPNL  KF  S   N +I  ++ G  T Y KL+G +D ++Y    HVR   + P AK FLRAGPR  LHF P EVR A+VTCGGLCPGLNSV+HHLV T+   Y A+KVFGI GGFAGF+DE  P  E++ + VE +QH+ GS+LGS+RGGFDLDKII F+   K+NQLYIIGGDGTHRGA+RIA+ECM R +N++V G+PKTIDND+DIIDRSFGF T+VE+AQ AI++ATTEA+C +PNG+GVVKLMGRS+G+IAA AT+ASGDVDLCLVPE  IVL+GP GC+PH+MKRV+EKGYAVVVVAEGAGEE+LGE+   DA+G+K+ P IG FMK  IE  +  + ++CNVKYIDPSYMIRSVAA ++DQIYCMQLAQNAVHGAMAGYTAFSAG+VN+RTVY+P+ ELV NSPR +N  GRTWE VLT+TRQP+T+ SRA
Sbjct:  219 IEEIVPVPVPNLKTKFKPSGVANLDIASDSLGKVTHYTKLIGEKDIVLY---DHVRGPGRMPAAKGFLRAGPRASLHFKPEEVRAAVVTCGGLCPGLNSVIHHLVLTLFNIYGAEKVFGIVGGFAGFWDENHPPKEMSVKDVELIQHEGGSILGSARGGFDLDKIIDFINKKKINQLYIIGGDGTHRGAVRIATECMARDMNVAVAGVPKTIDNDVDIIDRSFGFQTSVEAAQHAIESATTEARCAIPNGVGVVKLMGRSSGFIAAMATLASGDVDLCLVPECDIVLDGPRGCLPHIMKRVKEKGYAVVVVAEGAGEEILGETGETDATGHKKFPEIGPFMKTCIEKYFSDQGEVCNVKYIDPSYMIRSVAANSYDQIYCMQLAQNAVHGAMAGYTAFSAGMVNDRTVYLPMEELVANSPRIVNPLGRTWENVLTVTRQPSTLGSRA 662          

BLAST of mRNA_E-siliculosus-1a_F_contig1117.784.1 vs. uniprot
Match: A0A835ZIU1_9STRA (Pyrophosphate-dependent phosphofructose kinase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZIU1_9STRA)

HSP 1 Score: 584 bits (1506), Expect = 4.050e-205
Identity = 281/444 (63.29%), Postives = 350/444 (78.83%), Query Frame = 0

Query:    3 LDKIIMCEVPNLNVKFPSSLAPNREITVNTQGSPTCYNKLLGPRDCIIYGECQHVRDKNKPPTAKAFLRAGPRPELHFNPTEVRGAIVTCGGLCPGLNSVVHHLVETMLLTYKADKVFGIRGGFAGFYDEATPAVELT-SEGVEFLQHQPGSVLGSSRGGFDLDKIIKFLVDNKVNQLYIIGGDGTHRGALRIASECMQRGLNISVTGIPKTIDNDLDIIDRSFGFLTAVESAQAAIQAATTEAKCNLPNGIGVVKLMGRSAGYIAAYATMASGDVDLCLVPESPIVLEGPSGCIPHLMKRVEEKGYAVVVVAEGAGEELLGESTAKDASGNKRLPAIGKFMKGAIEDAYHAEDKICNVKYIDPSYMIRSVAATAFDQIYCMQLAQNAVHGAMAGYTAFSAGVVNNRTVYIPITELVENSPRCLNMHGRTWERVLTITRQPNTI 445
            L+ +++C+VPNL ++ P++   N EI  N+ G  T YNKLLG  DCIIY   +HVRDK K P A+AFLRAGPR +LHF+PT V   IVTCG LCPGLNSV+HHLV+T+   Y A K++GIRGG+ GF+D   P ++L  S+ +EFLQ + GS+LG S GGFD+ KI++FL+D +++ +Y+IGGDGTHRGA RIA EC+  GLNI+V GIPK++DND+D++DRSFGF+TAVE AQ AI++AT EAKCNLPNGIG+VKLMGRS GY+AAYATMASGDVDLCLVPE  I L+GP GC+PHL + V+ KGYAVVV++EGAG+  L E  A+  S    LP++G++MK  IE  + ++ K    KYIDPS  +RSVAA AFDQI CMQLAQNAVHGAMAGYTAF+AGVVNNRTV IPITE+V+ SP+ LN  GRTWERVL ITRQPNT+
Sbjct:   43 LETVMLCQVPNLALRMPTAGVANHEIVKNSSGGSTFYNKLLGDEDCIIY---EHVRDKTKAPVARAFLRAGPRAKLHFDPTTVNACIVTCGLLCPGLNSVIHHLVDTLKNNYNAGKIYGIRGGWGGFWDPEKPPIQLCQSKDLEFLQSRAGSILGCSVGGFDVKKIMQFLMDKQISHMYVIGGDGTHRGAHRIAVECIAMGLNIAVAGIPKSVDNDIDLVDRSFGFMTAVEEAQNAIRSATIEAKCNLPNGIGIVKLMGRSTGYLAAYATMASGDVDLCLVPEVEIRLDGPDGCLPHLERVVQRKGYAVVVISEGAGQAQLSEVAAEARSKGAPLPSVGQYMKSQIEKHFASKGKTATCKYIDPSVSVRSVAANAFDQILCMQLAQNAVHGAMAGYTAFAAGVVNNRTVLIPITEIVDTSPKGLNPRGRTWERVLCITRQPNTL 483          

BLAST of mRNA_E-siliculosus-1a_F_contig1117.784.1 vs. uniprot
Match: A0A836C7Q7_9STRA (Pyrophosphate-dependent phosphofructokinase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C7Q7_9STRA)

HSP 1 Score: 577 bits (1486), Expect = 2.420e-202
Identity = 284/448 (63.39%), Postives = 345/448 (77.01%), Query Frame = 0

Query:    4 DKIIMCEVPNLNVKFPSSLAPNREITVNTQGSPTCYNKLLGPRDCIIYGECQHVRDKNKPPTAKAFLRAGPRPELHFNPTEVRGAIVTCGGLCPGLNSVVHHLVETMLLTYKADKVFGIRGGFAGFYDEATPAVELTSEGVEFLQHQPGSVLGSSRGGFDLDKIIKFLVDNKVNQLYIIGGDGTHRGALRIASECMQRGLNISVTGIPKTIDNDLDIIDRSFGFLTAVESAQAAIQAATTEAKCNLPNGIGVVKLMGRSAGYIAAYATMASGDVDLCLVPESPIVLEGPSGCIPHLMKRVEEKGYAVVVVAEGAGEELLGESTAKDASGNKRLPAIGKFMKGAIEDAYHAEDKICNVKYIDPSYMIRSVAATAFDQIYCMQLAQNAVHGAMAGYTAFSAGVVNNRTVYIPITELVENSPRCLNMHGRTWERVLTITRQPNTIESRAGK 451
            DK+ MCEVPNLN  FP     N  +T  + G P+ +N+LLGPRDCI+    +HV    K    KA LRAGPRP LHF P E   AIVTCGGLCPGLNSV+H++ ET+L  Y A +V GI+GGF GFYD     +ELT + V  LQ+Q G++LGSSRGGFDLDKI+ FL  ++V  LY+IGGDGTHRGA +IA  C++R LNI+V GIPKTIDND+D++DRSFGF T+VE+AQ AI+ A TEA CNLPNGIG+VKLMGRS+G+IAAYATMASG+VDLCLVPE+PIVLEG  GC+PHL + V +KGYAVVVVAEGAGEELLG S   DASGNK+LP IG+FM+  IE+ +  + K C+ KYIDPSYM+RSV A A DQI CMQLAQNAVH AMAGYT+F+ G+VNN+ V IPI+ +   SPR L+ +GRTWER+++IT QPNT+  R  K
Sbjct:   14 DKVYMCEVPNLNEAFPPCNMRNDCMTQQSTGEPSLWNRLLGPRDCILL---EHVCGPGKARKLKALLRAGPRPLLHFPPKETVAAIVTCGGLCPGLNSVIHYVTETLLQNYGARRVIGIKGGFGGFYDADLEPLELTMDAVNGLQNQGGTLLGSSRGGFDLDKILLFLQKHEVTHLYVIGGDGTHRGADKIAHACLERKLNIAVAGIPKTIDNDVDLLDRSFGFTTSVEAAQNAIKVAVTEATCNLPNGIGIVKLMGRSSGFIAAYATMASGEVDLCLVPEAPIVLEGDKGCLPHLERVVADKGYAVVVVAEGAGEELLGASAETDASGNKKLPKIGEFMQAKIEEYFAKQGKQCSPKYIDPSYMVRSVPANASDQILCMQLAQNAVHAAMAGYTSFTVGMVNNKVVLIPISLITATSPRKLDPYGRTWERIISITLQPNTVPFRPVK 458          

BLAST of mRNA_E-siliculosus-1a_F_contig1117.784.1 vs. uniprot
Match: A0A835YV15_9STRA (Pyrophosphate-dependent phosphofructose kinase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YV15_9STRA)

HSP 1 Score: 555 bits (1431), Expect = 1.230e-193
Identity = 271/450 (60.22%), Postives = 340/450 (75.56%), Query Frame = 0

Query:    3 LDKIIMCEVPNLNVKFPSSLAPNREITVNTQGSPTCYNKLLGPRDCIIYGECQHVRDKNKPPTAKAFLRAGPRPELHFNPTEVRGAIVTCGGLCPGLNSVVHHLVETMLLTYKADKVFGIRGGFAGFYDEATPAVELTSE-----GVEFLQHQPGSVLGSSRGGFDLDKIIKFLVDNKVNQLYIIGGDGTHRGALRIASECMQRGLNISVTGIPKTIDNDLDIIDRSFGFLTAVESAQAAIQAATTEAKCNLPNGIGVVKLMGRSAGYIAAYATMASGDVDLCLVPESPIVLEGPSGCIPHLMKRVEEKGYAVVVVAEGAGEELLGESTAKDASGNKRLPAIGKFMKGAIEDAYHAEDKICNVKYIDPSYMIRSVAATAFDQIYCMQLAQNAVHGAMAGYTAFSAGVVNNRTVYIPITELVENSPRCLNMHGRTWERVLTITRQPNTIES 447
            L  I++C+VP L+ K PS+  PN EI  NT G PT YN+L G  +CII    +HVRD+ K P AKAFLRAGPR +LHF+P +V  A+VTCG LCPGLNSV+HHL +T+   Y A KV+G+RGG+ GF++  TP +EL  +     G E LQH+ GS+LG + GGFDL KI+ F+   +++QLY+IGGDG  RGA RIA EC     N+S+ GIPK+++ND+DIIDRSFGF+TAVE+AQ AI+ AT EAKCN+PNGIG+VKLMGRS GY+AAYATMASGDVDLCLVPE P+VLEG +GC+PHL + V+++GYAVVVV++GAG+  L E+ A+  +  +  P+IG F+K  IE  +  + +   VKYIDPS  +RSVAA AFDQI C+QLAQNAVHG MAGYTAF AGV NNRT  IP+TE+ ENSP+ LN HGRTWERVL IT QP T E+
Sbjct:   43 LTTIMLCQVPRLSAKLPSAGVPNLEIKSNTSGQPTYYNRLTGDTECIID---EHVRDERKAPVAKAFLRAGPRAKLHFDPVKVNAAVVTCGTLCPGLNSVIHHLRDTLTTIYGAQKVYGVRGGWGGFHNPHTPVIELHDDALDVSGRESLQHRAGSILGCAAGGFDLYKIMDFIKRKEISQLYVIGGDGAQRGAHRIAVECAALNWNVSIAGIPKSVNNDIDIIDRSFGFMTAVEAAQNAIRCATIEAKCNVPNGIGIVKLMGRSTGYLAAYATMASGDVDLCLVPEVPVVLEGKNGCLPHLERVVKKQGYAVVVVSDGAGQPALSEAAAEARAAGQPAPSIGPFLKCEIEAYFKRKCRPVTVKYIDPSVAVRSVAANAFDQILCLQLAQNAVHGVMAGYTAFCAGVCNNRTCMIPMTEITENSPKSLNPHGRTWERVLCITGQPGTHET 489          

BLAST of mRNA_E-siliculosus-1a_F_contig1117.784.1 vs. uniprot
Match: K8Z818_NANGC (6-phosphofructo-2-kinase / fructose-2,6-bisphosphatase (Fragment) n=3 Tax=Monodopsidaceae TaxID=425072 RepID=K8Z818_NANGC)

HSP 1 Score: 545 bits (1403), Expect = 8.440e-189
Identity = 272/439 (61.96%), Postives = 337/439 (76.77%), Query Frame = 0

Query:    8 MCEVPNLNVKFPSSLAPN-REITVNTQGSPTCYNKLLGPRDCIIYGECQHVRDKNKPPTAKAFLRAGPRPELHFNPTEVRGAIVTCGGLCPGLNSVVHHLVETMLLTYKADK-VFGIRGGFAGFYDEATPAVELTSEGVEFLQHQPGSVLGSSRGGFDLDKIIKFLVDNKVNQLYIIGGDGTHRGALRIASECMQRGLNISVTGIPKTIDNDLDIIDRSFGFLTAVESAQAAIQAATTEAKCNLPNGIGVVKLMGRSAGYIAAYATMASGDVDLCLVPESPIVLEGPSGCIPHLMKRVEEKGYAVVVVAEGAGEELLGESTAKDASGNKRLPAIGKFMKGAIEDAYHAEDKICNVKYIDPSYMIRSVAATAFDQIYCMQLAQNAVHGAMAGYTAFSAGVVNNRTVYIPITELVENSPRCLNMHGRTWERVLTITRQPNT 444
            + E+PNL   F  +   N  +++  T G    YNKLLG +D II      VR +  PP  K ++RAGPR ELHF P +VR AIVTCGGLCPGLN+V+  LV ++   Y+A   V+GIRGGF+GF+D     + LT E V+ + H+ G++LGSSRGGFD+DKII FL + ++ QLYIIGGDGTHRGA +I  EC++RGLNI+V GIPKTIDND+D+IDRSFGF T+VE+AQAAI+ A TEA CNLPNGIGVVKLMGRSAG+IA +AT+ASGDVDLCL+PE PIVLEG  GC+PHL +RV++KG+AVVVVAEGAGEELLG+S  KDASGNK+LPAIG+F+K  I   + ++ +   VKYIDPSYM+RSV A A D IYC  LAQNAVHGAMAG+T F+ G+ NNR VY+PI  +V  SPR ++  GRTWERVL++TRQPNT
Sbjct:   90 LLEIPNLGKTFKGAHVQNLSQMSGTTSGESIAYNKLLGHQDGII---ADIVRREGLPPETKVYVRAGPRKELHFEPEKVRAAIVTCGGLCPGLNNVIRELVNSLFHLYRAQSGVYGIRGGFSGFHDAHRLPIMLTPELVDQIHHEGGTILGSSRGGFDIDKIIHFLEEKQICQLYIIGGDGTHRGANKIGEECLKRGLNIAVVGIPKTIDNDVDLIDRSFGFQTSVEAAQAAIRCAKTEAICNLPNGIGVVKLMGRSAGFIAVHATLASGDVDLCLIPEVPIVLEGERGCLPHLAQRVQQKGHAVVVVAEGAGEELLGQSAEKDASGNKKLPAIGQFLKEKIGIYFKSQGQPSTVKYIDPSYMVRSVPANAADSIYCSLLAQNAVHGAMAGFTQFTVGLCNNRLVYLPIPLVVATSPRVMDPVGRTWERVLSVTRQPNT 525          

BLAST of mRNA_E-siliculosus-1a_F_contig1117.784.1 vs. uniprot
Match: A0A835ZA90_9STRA (Pyrophosphate-dependent phosphofructokinase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZA90_9STRA)

HSP 1 Score: 540 bits (1390), Expect = 7.900e-187
Identity = 268/448 (59.82%), Postives = 337/448 (75.22%), Query Frame = 0

Query:    5 KIIMCEVPNLNVKFPS-SLAPNREITVNTQGSPTCYNKLLGPRDCIIYGECQHVRDKNKPPTAKAFLRAGPRPELHFNPTEVRGAIVTCGGLCPGLNSVVHHLVETMLLTYKADKVFGIRGGFAGFYDEATPAVELTSEGVEFLQHQPGSVLGSSRGGFDLDKIIKFLVDNKVNQLYIIGGDGTHRGALRIASECMQRGLNISVTGIPKTIDNDLDIIDRSFGFLTAVESAQAAIQAATTEAKCNLPNGIGVVKLMGRSAGYIAAYATMASGDVDLCLVPESPIVLEGPSGCIPHLMKRVEEKGYAVVVVAEGAGEELLGESTAKDASGNKRLPAIGKFMKGAIEDAYHAEDKICNVKYIDPSYMIRSVAATAFDQIYCMQLAQNAVHGAMAGYTAFSAGVVNNRTVYIPITELVENSPRCLNMHGRTWERVLTITRQPNTIESRAGK 451
            +I + EVP L+  FPS ++  +  +   + GSP  +N+LLGP DC+I      +   ++P   KA LRAGPRP LHF+P +   AIVTCGGLCPGLNSV+HHL ET++  Y A +V G+RGGFAGFY+     ++LT   V+ LQHQ G++LG+SRGGF+LD II FL   +V QLY+IGGDGTHR A +IA EC  + LNI+V G+PKTIDND+D++DRSFGF T+VE+AQAAI +A TEA CNLPNGIG+VKLMGRS+G+IAA+AT+ASGDVDLCLVPE P+VLEGP GC+PHLM+RV +KGYAVVVVAEGAGE +LG S   D SGNK+LP + +FM+ +IED +H  +     K IDPSY IRSV A A DQI CMQLAQNAVH AMAGYTAF+ G+VNN+ V IP++ +   SPRCL+ +GR  ERVL+ T QP+T+  R  K
Sbjct:   76 QIYLAEVPCLSDYFPSMNIRNDMMVQALSGGSPAFWNRLLGPDDCVIIDP---ICGPDRPGHKKALLRAGPRPLLHFDPQKTVAAIVTCGGLCPGLNSVIHHLTETLVKNYSAQRVIGVRGGFAGFYNRQLTPLDLTVSRVDRLQHQGGTLLGTSRGGFELDNIIAFLRAYQVTQLYVIGGDGTHRAAQKIAMECKAQQLNIAVAGVPKTIDNDVDMLDRSFGFETSVETAQAAITSAVTEASCNLPNGIGIVKLMGRSSGFIAAHATLASGDVDLCLVPEMPLVLEGPKGCLPHLMQRVHDKGYAVVVVAEGAGENVLGSSAEVDLSGNKKLPKVAEFMQHSIEDFFHRHNLPVTCKSIDPSYTIRSVPANASDQILCMQLAQNAVHAAMAGYTAFTVGLVNNKAVLIPMSLVTATSPRCLDPYGRVLERVLSTTLQPSTLPYRPPK 520          

BLAST of mRNA_E-siliculosus-1a_F_contig1117.784.1 vs. uniprot
Match: A0A1Z5KRF2_FISSO (6-phosphofructokinase 1 n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5KRF2_FISSO)

HSP 1 Score: 535 bits (1377), Expect = 2.060e-184
Identity = 270/468 (57.69%), Postives = 343/468 (73.29%), Query Frame = 0

Query:    1 MFLDKIIMCEVPNLNVKFPSS------------LAPNREITVNTQGSP---TCYNKLLGPRDCIIYGECQHVRDKNKPPTAKAFLRAGPRPELHFNPTEVRGAIVTCGGLCPGLNSVVHHLVETMLLTYK-ADKVFGIRGGFAGFYDEATPAVELTSEGVEFLQHQPGSVLGSSRGGFDLDKIIKFLVDNKVNQLYIIGGDGTHRGALRIASECMQRGLNISVTGIPKTIDNDLDIIDRSFGFLTAVESAQAAIQAATTEAKCNLPNGIGVVKLMGRSAGYIAAYATMASGDVDLCLVPESPIVLEGPSGCIPHLMKRVEEKGYAVVVVAEGAGEELLGESTAKDASGNKRLPAIGKFMKGAIEDAYHAEDKICNVKYIDPSYMIRSVAATAFDQIYCMQLAQNAVHGAMAGYTAFSAGVVNNRTVYIPITELVENSPRCLNMHGRTWERVLTITRQPNTI-ESRAGK 451
            +F+DK+ + E  +L   F S+            + P    T+  + S    T YNKL+   +CI+ G+   VR  N+     A++RAGPR  LHF+P  V  AIVTCGGLCPGLN+V+  + +++   Y    KV+GIRGG+ GF+D     VELT + VE + H  G+VLGSSRGGFDLDKI+ F+ D K++QL++IGGDGTHRGA RI   C++RG N++V GIPKTIDND+D ID+SFGF +AVE+AQ AI++A TEA CNLPNGIG+VKLMGRSAG+IAA++TMAS DVDLCLVPE P+VL+G  GC+PHLM+RV+++GYAVVVVAEGAGEE+LG ST  DASGNK+LPAIG+FMK +IE+ +    +   VKYIDPSY +R+V A A D +YCMQLAQNAVHGAMAGYT FS G+ NNR V++PI ELV  SPR +N  GRTWERV+ +TRQPNT+ E   GK
Sbjct:   84 VFIDKVFLWECQDLRKSFASANVANHMHFQYETIHPLASTTLQRKNSTEIITQYNKLITDHECIL-GDV--VRKPNQQTNTVAYMRAGPRRYLHFDPAHVNAAIVTCGGLCPGLNNVIREITKSLYQLYAIGGKVYGIRGGYRGFWDPELEPVELTLDLVENIHHGGGTVLGSSRGGFDLDKILDFVRDRKISQLFVIGGDGTHRGAFRIHEGCIERGYNVAVAGIPKTIDNDVDYIDQSFGFQSAVEAAQVAIRSAKTEAVCNLPNGIGIVKLMGRSAGFIAAHSTMASSDVDLCLVPEVPVVLDGERGCLPHLMRRVKQQGYAVVVVAEGAGEEVLGTSTEVDASGNKKLPAIGEFMKKSIEEYFKKHGETATVKYIDPSYTVRTVPANAADSLYCMQLAQNAVHGAMAGYTGFSVGLCNNRMVFLPIPELVATSPRSMNARGRTWERVIGLTRQPNTVPELEEGK 548          

BLAST of mRNA_E-siliculosus-1a_F_contig1117.784.1 vs. uniprot
Match: B7G4J8_PHATC (Pyrophosphate-dependent phosphofructose kinase n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7G4J8_PHATC)

HSP 1 Score: 531 bits (1368), Expect = 5.620e-183
Identity = 271/472 (57.42%), Postives = 334/472 (70.76%), Query Frame = 0

Query:    3 LDKIIMCEVPNLNVKFPSSLAPNR-------------------------EITVNTQGSPTCYNKLLGPRDCIIYGECQHVRDKNKPPTAKAFLRAGPRPELHFNPTEVRGAIVTCGGLCPGLNSVVHHLVETMLLTYKAD-KVFGIRGGFAGFYDEAT--PAVELTSEGVEFLQHQPGSVLGSSRGGFDLDKIIKFLVDNKVNQLYIIGGDGTHRGALRIASECMQRGLNISVTGIPKTIDNDLDIIDRSFGFLTAVESAQAAIQAATTEAKCNLPNGIGVVKLMGRSAGYIAAYATMASGDVDLCLVPESPIVLEGPSGCIPHLMKRVEEKGYAVVVVAEGAGEELLGESTAKDASGNKRLPAIGKFMKGAIEDAYHAEDKICNVKYIDPSYMIRSVAATAFDQIYCMQLAQNAVHGAMAGYTAFSAGVVNNRTVYIPITELVENSPRCLNMHGRTWERVLTITRQPNTIE 446
            LDKI + E  +L  KFPS++  N                          E    T    T YNKL+   +CI+      VR  N      +++RAGPR  LHF+P  V  AIVTCGGLCPGLN+V+  + +T+   Y  +  V+GI+GGF GFYD A     V LT E VE + H+ G+VLGSSRGGFD++KI  F+  +K+N LY+IGGDGTHRGA RI  +CM+ G+N++V GIPKTIDND+D ID SFGF +AVE+AQ AI++A TEA CNLPNGIG+VKLMGRSAG+IAA+ATMAS DVDLCLVPE P VLEGP+GC+PHLM+RV+++GYAVVVVAEGAGEE+LG S   DASGNK+LPAIG+FMK A+ D +     +  VKYIDPSY +RSV A A D +YCMQLAQNAVHGAMAG+T FS G+ NNR V++PI ELV  SPR +N  GRTWERVL +TRQPNT++
Sbjct:   78 LDKIYLWECEDLRKKFPSAMTENTMKCKATPAPLIRSPTLRRSYTAELDESDFETAEKITRYNKLITDHECIL---ADIVRKPNMHTHTVSYMRAGPRRHLHFDPQSVNAAIVTCGGLCPGLNNVIREITKTLHQIYGIEGTVYGIQGGFRGFYDPAPHLQPVILTPELVENIHHEGGTVLGSSRGGFDIEKIRNFIHKHKINNLYVIGGDGTHRGAFRIHEDCMEHGMNVAVAGIPKTIDNDVDYIDHSFGFNSAVEAAQYAIRSAKTEAVCNLPNGIGIVKLMGRSAGFIAAHATMASSDVDLCLVPEVPTVLEGPNGCLPHLMRRVKQQGYAVVVVAEGAGEEVLGISAEADASGNKKLPAIGEFMKQAVTDFFAKHGDVATVKYIDPSYTVRSVPANAADSLYCMQLAQNAVHGAMAGFTGFSVGLCNNRMVWLPIPELVATSPRSMNPRGRTWERVLALTRQPNTVQ 546          

BLAST of mRNA_E-siliculosus-1a_F_contig1117.784.1 vs. uniprot
Match: A0A7S0PJW2_9STRA (Hypothetical protein n=1 Tax=Leptocylindrus aporus TaxID=1398097 RepID=A0A7S0PJW2_9STRA)

HSP 1 Score: 529 bits (1362), Expect = 2.940e-182
Identity = 251/413 (60.77%), Postives = 318/413 (77.00%), Query Frame = 0

Query:   33 QGSPTCYNKLLGPRDCIIYGECQHVRDKNKPPTAKAFLRAGPRPELHFNPTEVRGAIVTCGGLCPGLNSVVHHLVETMLLTYKADKVFGIRGGFAGFYDEATPAVELTSEGVEFLQHQPGSVLGSSRGGFDLDKIIKFLVDNKVNQLYIIGGDGTHRGALRIASECMQRGLNISVTGIPKTIDNDLDIIDRSFGFLTAVESAQAAIQAATTEAKCNLPNGIGVVKLMGRSAGYIAAYATMASGDVDLCLVPESPIVLEGPSGCIPHLMKRVEEKGYAVVVVAEGAGEELLGESTAKDASGNKRLPAIGKFMKGAIEDAYHAEDKICNVKYIDPSYMIRSVAATAFDQIYCMQLAQNAVHGAMAGYTAFSAGVVNNRTVYIPITELVENSPRCLNMHGRTWERVLTITRQPNTI 445
            +G+P  YNK++G ++ I+      VR  +    +KAF+RAGPR +LHFNP EV  AIVTCGGLCPGLN+VV  L  T++  Y AD V+GIRGG+ GFYD     ++L  + V  + H+ G+ LGSSRGGFDL++I  FL    ++QLY+IGGDGTHRGA +I   CM+RG+N++V GIPKTIDND+D +DR+FGF TAVE++  AI+ A  EA CN PNG+G+VKLMGR AGYIAA+ATM S DVDLCL+PE PIVLEGP GC+PH+ +RV+E+G+AV+VVAEGAGEE++G ST  DASGNK+LPAIG+F+K A+ D + ++ +   VKYIDPSY IRSV A A D +YCMQLAQNAVHGAMAGYT FS G+VNN+T  +PI ELV  SP+ +N  GRTWERVL +TRQPNT+
Sbjct:  140 KGTP--YNKIIGEKEVIL---ADVVRKPDGSTVSKAFIRAGPREKLHFNPDEVNAAIVTCGGLCPGLNNVVRELTNTLINLYGADTVYGIRGGYKGFYDPEYSPIQLDPDVVSTIHHEGGTFLGSSRGGFDLERICNFLKAKNISQLYVIGGDGTHRGAFKIHEGCMERGMNVAVAGIPKTIDNDIDHVDRTFGFDTAVEASLKAIRCAKVEAMCNKPNGVGIVKLMGREAGYIAAHATMCSYDVDLCLIPEVPIVLEGPLGCLPHIRQRVKEQGFAVIVVAEGAGEEIIGSSTETDASGNKKLPAIGEFLKKAVTDYFKSKGEEATVKYIDPSYTIRSVPANAADSVYCMQLAQNAVHGAMAGYTGFSTGIVNNKTALLPIPELVATSPKAMNPRGRTWERVLALTRQPNTV 547