prot_E-siliculosus-1a_F_contig110.690.1 (polypeptide) Ectocarpus siliculosus Ec863f_EcPH12_90f female

BLAST of mRNA_E-siliculosus-1a_F_contig110.690.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 1958 bits (5073), Expect = 0.000e+0
Identity = 995/1017 (97.84%), Postives = 1003/1017 (98.62%), Query Frame = 0

Query:   13 MLVASSSPVLGVDLGKIQAITASSAFDDFARSQSAYSAASSWYTDRLSYDAAEGSRAPYIACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAAGGESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDSLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRAACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYISDYLGDPYDYDDSEYTSYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSSLEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYSCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLSEDEYLYDTFMYENPEHLLIFAAGNDGGYEDVPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVMSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLFVIEAAIADGEITELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVISPSGAKYTMWESGEDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAIYSSK 1029
            MLVASSSPVLGVDLGKIQAITASSAFDDFARSQSAYSAASSWY DRLSYDAAEGS APY+ACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTA G ESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVD LASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETR ACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGF FDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTY+SDYLGDPYDYDDSEY SYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQS LEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMY+CDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQL+EDEYLYDTFMYENPEHLLIFAAGNDGGYED+PNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNL YEELGLTDYSPEGYPWICL PFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTD HSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDV+SRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGL V+EAAIADGEITELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVI+PSGAKYTMWESG DDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAIYSSK
Sbjct:    1 MLVASSSPVLGVDLGKIQAITASSAFDDFARSQSAYSAASSWYNDRLSYDAAEGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAAIADGEITELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAIYSSK 1017          

BLAST of mRNA_E-siliculosus-1a_F_contig110.690.1 vs. uniprot
Match: D8LU20_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LU20_ECTSI)

HSP 1 Score: 1627 bits (4212), Expect = 0.000e+0
Identity = 855/1036 (82.53%), Postives = 898/1036 (86.68%), Query Frame = 0

Query:    1 MGLHPDHVAAFLMLVASSSPVLGVDLGKIQAITASSAFDDFARSQSAYSAASSWYTDRLSYDAAEGSRAPYIACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAAGGESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDSLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRAACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYISDY----LGDPYDYDDSEYTSYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSF--TGGFHKDTVGGHGTWTAGSAAGAISAQSSLEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYSCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLSEDEYLYDTFMYENPEHLLIFAAGNDGGYEDVPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVMSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLFVIEAAI-ADGEITELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVISPSGAKYTMWESGEDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAIYSSK 1029
            MGL  DHVAA L LVAS S V+GVDLGKIQ ITASSAFDDFARSQSAYSAAS W+T RL++ A EGSRAPYIACADYKQGRHA+TSLETAFT+SAVHRVSNTE DG+CFIVTALPSSATA+I APEDF LLSAAP LPSLKLAT LLDHGPDTSP +GAADDF+LSD SAPLRSTYGE                                                         DRS+YDVTRVREWSRAAAVVDSLASK A+T GEICGFGKL LRHVADDL+LVEGMNHLLPGK+ PSE R ACYMGLLSQLAAKPEVLRVSPLQSA L+NAVANALVQSATTTETPLLDAGLDGTGEVIQVVD+GLDETSCFFADDDGLQVEHGF F GVRVE DGNVSTVLGNYSFPFDMSRRKIVQYIQ+FKTPDQGSTSSGQ+SFTY SDY    LGD +D DDSEY SYDDYSGEY SDTWSE+SFCA VLDDLSYHFS LFDDTSYS SS S S   TGGF KD VGGHGTWTAG AAGAISAQSSL+EEACYGDELPGCAGGCISASDVDAMLD+ +FDLDVFCPMY+CDGNTDIPSSECLSDDPVENLHQHGGVAPGAQIS+ D SY G++ +A FAGN LWYSAMETGAKIH+NSWG   LCQL+E+EYLYD+FMYENPEHLLIFAAGN GG ED+PN E CT+GSPALGKN LAVGATSSGP  GTRTG+DGNL YEE G+TDYSPEGYPWICL P LGTPSAS EPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTD HSC+L+A+MGTSASCPLVAGAAALVRQYFKDPSFFAKDV SRGGCAEHSSS QCSEFAPSAATVKAMIINSAHLMGGSSEPNG+RGFGRVHLEAGMPMDGVGETGL V+EA I +D EITELVTVDGDAG ELRATLAWLDPPTTALSAKQLQHDLDLHVI+PSGAKYTMWESG DDTVNVVERVIVPVESMESGVWSVVVSAK LLTDEQ YSLVVTGAIYSSK
Sbjct:    1 MGLRADHVAAIL-LVASPSAVIGVDLGKIQTITASSAFDDFARSQSAYSAASKWHTKRLAH-APEGSRAPYIACADYKQGRHAMTSLETAFTESAVHRVSNTETDGSCFIVTALPSSATAMISAPEDFALLSAAPFLPSLKLATRLLDHGPDTSPHVGAADDFNLSDLSAPLRSTYGE---------------------------------------------------------DRSQYDVTRVREWSRAAAVVDSLASKRAQTFGEICGFGKLSLRHVADDLLLVEGMNHLLPGKKAPSEARTACYMGLLSQLAAKPEVLRVSPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQVEHGFLFGGVRVEGDGNVSTVLGNYSFPFDMSRRKIVQYIQMFKTPDQGSTSSGQNSFTYASDYIRDYLGDAFDDDDSEYNSYDDYSGEYSSDTWSEESFCAQVLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLQEEACYGDELPGCAGGCISASDVDAMLDNGSFDLDVFCPMYNCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDMLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTIGSPALGKNILAVGATSSGPSRGTRTGEDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEATIGSDDEITELVTVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESMESGVWSVVVSAKGLLTDEQSYSLVVTGAIYSSK 977          

BLAST of mRNA_E-siliculosus-1a_F_contig110.690.1 vs. uniprot
Match: A0A6H5JQ52_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQ52_9PHAE)

HSP 1 Score: 1094 bits (2829), Expect = 0.000e+0
Identity = 575/753 (76.36%), Postives = 606/753 (80.48%), Query Frame = 0

Query:  111 VTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAAGGESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDSLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRAACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTE---TPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRVEDDGNVSTVLGNYSFPFDMSRRK-------------------------------------------------IVQYIQLFKTPDQGSTSSGQDSFTYISDYLGDPYDYDDSEYTSYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSSLEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYSCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLSEDEYLYDTFMYENPEHLLIFAAGNDGGYEDVPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQV-ASASSDGTDEHSCKL 810
            V ALPSSATAVIRAPEDFDLLS  PLL SLKLATSLLDHGPDTSPRLGAAD+ DLSDHSAPLRSTYGEG                                          VNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVD LASKSARTVGEICGFGKLKLRHVADDLVLVEGM +LLPGKRFPSETR ACYMGLLSQLAAKPEVL VSPLQSA LHNAVANA +QSAT  +    P    G           DSGLD+TSCFFADDDGLQVEHG+ FDGVRVEDDGNVST LGNYSFPFDMSRRK                                                 IVQ IQLFKTP QGSTSSGQDSFT+ SD++GDP+DYDDSEY SY DYSGEYYSDTWSEDSFCAG LDDLSYH SYL+DDTSY+S STSPSFTGGFH+DT+GGHGT T+GSAAGAIS+QSSL+EEACYGDELPGCAGGCISASDVDAMLDDSTF L VFCPMY+CDGN DIPSSECLSDDPVE+LHQHGGVAPGAQISVFD SYVGTE+YAS+AGNDLWYSAMETGAKIHTNSWGGATLCQL+E EY+YDTFMYENPEHLLIF+AGNDGGY+D+PNREACTVGSPALGKN LAVGATSSGPFPGTRTGDDGNLF EE+G+TDYSPEGYPWICLMP LG P ASTEPAGVDTV +FSSQGPTLDG+IKP VVAPG+QV ASASSDGTDEHSCKL
Sbjct:    5 VAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGEG------------------------------------------VNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 711          

BLAST of mRNA_E-siliculosus-1a_F_contig110.690.1 vs. uniprot
Match: A0A6H5KDF8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KDF8_9PHAE)

HSP 1 Score: 691 bits (1783), Expect = 8.010e-238
Identity = 354/489 (72.39%), Postives = 378/489 (77.30%), Query Frame = 0

Query:  409 YSFPFDMSRRKIVQYIQLF---KTPDQGSTSSGQDSFTYISDYLGDPYDYDDSEYTSYDDYSGEYYSDT-------------------------------------------------W-----------------------------------SEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSSLEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYSCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLSEDEYLYDTFMYENPEHLLIFAAGNDGGYEDVPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 810
            +S P+  +   +V Y  +    KTPDQGSTSSGQDSFTY SDY GDPYDYDDSEY S+DDYSGEY+SDT                                                 W                                   SE+    G LDDL+YH SYL+DDTSY+SSSTSPSFTGGFHKDT+GGHGT TAGSAAGAIS+QSSLEEEACYGDELP CAGGCISASDV+AMLDDSTFDLDVFCPMY+CDGNTDIPSSECLSDDPVENLHQHGG APGAQISVFDVSYVGTE+YAS+AGNDLWYSAMETGAKIHTNSWGGATLCQL+E EY+YD FMYENPEHLLIF+AGNDGGYED+PNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEE+GLTDYSPEGYPWICLMP+LGTPSASTEPAGVDTVA+FSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL
Sbjct:   15 FSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 503          

BLAST of mRNA_E-siliculosus-1a_F_contig110.690.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 673 bits (1737), Expect = 5.500e-224
Identity = 436/1020 (42.75%), Postives = 577/1020 (56.57%), Query Frame = 0

Query:   34 ASSAFDDFARSQSAYSAASSWYTDRLSYDAAEGSRAPYIACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAAGGESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEY-----DVTRVREWSRAAAVVDSLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFP--------SETRAACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRVEDDGNVST----VLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYISDYLGDPYDYDDSEYTSYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSSLEEEACYGDELPGCAGGCISASD-----VDAMLDDSTFDLDVFCPMYSCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYA--SFAGNDLWYSAMETGAKIHTNSWGGATLCQLSEDEYLYDTFMYENPEHLLIFAAGNDGGYEDVPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVMSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLFVIEA---AIADGEITE-LVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVISPSGAKYTMWESGEDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAI 1025
            A++A DDF     A+ AA++W  +RL     +G+RAP++ CA+Y  GR   + L+   +  AV  VS++E  G CF+ TA  + A+A++   E F L S +P   +LKLA  L+DH            + + S     L + +G  M + NV GL+V+L+P  L A      AE+F+ D   DL S SV++   +FWSD      E+        R R+WS+AA+VV  L+  +  + G+IC + ++ L    DD +LV G++HLL   R          +E   AC+MGL+S  A +PEVLRVSP  + R+ N  A  ++QSA+ T+TPL DAGLDGTGEVIQVVD+GLDETSCFF DD G +V+HG++F+ + V   G+       V     F +   RRK++QYI+L                              DS+Y ++    G  Y                      +L  D               F +D   GHGT TAGSAAGA       E   C GDE  GC GGCI  S+     V   +  +  DLD  CPMY CDG+TD     CLSD+  E L ++GG+A GA++S+FDV +   E++      GN +W   +E G K+H+ S GG TLC   E E LYD FMYENPE+LLIFAAGNDG  E    R  CT+ SPA+GKN LAVGA++SG         D  +   E G                           AG+D VA FSS G T DGRIKP+VVAPGD V SA+SDGTD HSC+L A  GTS SCP+VAGAAA+VRQYF D +F+  DV +RG C++      C  F+PSAATVKA++INSA+LMG SS+P+  RGFGR+HLE GMP++G G+  LFV +A   +IA+  + E L  VD DAG+ELRATL+W+D P T+ SA QL +DLDL V+SPSG  Y MW S   D  NV ERVIV  + +E+G W+V+V + +L+TD Q YSLVV GAI
Sbjct:   42 ATTAKDDFNGHARAFEAAATWNGERLRRSQ-DGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREV------EGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAG--AESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTGSSQLIYYPVFEAGDFTYTEERRKVIQYIELVNP---------------------------DSDYVAFSTTQGGVYP---------------------FLPPDE--------------FGQDDSAGHGTHTAGSAAGAT-LNDPAETLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGSTD----PCLSDEVDETLTKNGGMAQGAKLSIFDVFF---EVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETE----RSVCTMASPAIGKNVLAVGASTSG---------DTRVISTEGG---------------------------AGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSD---GFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLVTDSQSYSLVVNGAI 939          

BLAST of mRNA_E-siliculosus-1a_F_contig110.690.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 637 bits (1643), Expect = 2.830e-210
Identity = 426/1046 (40.73%), Postives = 565/1046 (54.02%), Query Frame = 0

Query:    7 HVAAFLMLVASSSPVLG----VDLGKIQAITASSAFDDFARSQSAYSAASSWYTDRLSYDAAEGSRAPYIACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAAGGESFAEAFLRDWHADLTSESVNMRRASFWSDSDAD------RSEYDVTRVREWSRAAAVVDSLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFP--------SETRAACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYISDYLGDPYDYDDSEYTSYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSSLEEEACYGDELPGCAGGCISASDV-DAMLDDST----FDLDVFCPMYSCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLSEDEYLYDTFMYENPEHLLIFAAGNDGGYEDVPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVMSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLFVIEA---AIADGEITE-LVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVISPSGAKYTMWESGEDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAI 1025
            H  A L+ + SS+   G      L  ++    ++A DDF     A+ AA++W  +RL +   E +RAP++ACA Y  G  A + L+   +  AV  VS++E  G CF  +A  + A A++   E F L + +P    LKLA  L+DH          A++   S     L + +G  M   NV GLSV L+PG L A   E  A++F+     DL S SV++   +FWSD   D       S     R R+W +AA+VV  L+  ++   G+IC + ++ + H  DD +LV G++HLL   R          +E   AC+MGL+S LA +PEVLRVSP  + R+ NA A  ++QSA+ T+TPL DAGLDGTGE+IQV+DSGLDETSC+F DD G +V+HG++FD +     G  +    +YS                    D+ +T      F+Y                                                                     + +    GHGT TAGSAAGA       E   C   +   C G CI   D  D ++  S+     DLD  CPMY CD +TD   + CLSDD  E L  +GG+A GA++++FDV Y G +  +S  GN+LW    E G K+H+NS G    C ++  + L D FMYEN E+LLIFAAGN+GG E    R  CT+ +P +GKNSLA+GA++SG    TR           +G T                          G+DTVA FSS G T D RIKP+VVAPGD V SA+SDGTD HSC+L A  GTS SCP+VAGAAA++RQYF D +F+  DV +RG C++      C  F+PSAATVKA++INSA+LMGGSSEP+G RGFGRVHLEAGMP++G G+  LFV +A   +IA G + E L  VD DAG++LRATL+W+DPP TA SA QL H+LDL V+SP G  Y MW S   D  NV ERVIV    + +G W+V V A  L+TD Q YSLVV GAI
Sbjct:    6 HRLALLVFLCSSAVAAGRLNSPALELVENALLTTAKDDFKGHVGAFEAAATWNGERLRHSQEE-TRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESHD----EAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSE--AKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDEL-----GRAAVYSSSYS-------------------SDETATIFNGGDFSY---------------------------------------------------------------------YPERRKAGHGTHTAGSAAGAT-LNDPAETITCNVTDTLSCVGACIDLLDATDDLVSISSQTDGTDLDRLCPMYGCDNSTD---TTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSI-GNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIE----RSTCTINTPGIGKNSLAIGASTSGD---TRV-------ISTVGAT--------------------------GIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSD---GFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDSQSYSLVVNGAI 903          

BLAST of mRNA_E-siliculosus-1a_F_contig110.690.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 617 bits (1590), Expect = 4.270e-201
Identity = 424/1044 (40.61%), Postives = 565/1044 (54.12%), Query Frame = 0

Query:   39 DDFARSQSAYSAASSWYTDRLSYDAAEGSRAPYIACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAAGGESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEY-----DVTRVREWSRAAAVVDSLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFP---------SETRAACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFA-DDDGLQVEHGFWFD--GV-----------------------RVEDD---GNVSTVLGNYS---FPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYISDYLGDPYDYDDSEYTSYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSSLEEEACYGDELPGCAGGCIS---ASDVDAMLDDST--FDLDVFCPMYSCDGNTDIPSSE-CLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLSEDEYLYDTFMYENPEHLLIFAAGNDGGYEDVPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGT-DEHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVMSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLFVIEA---AIADGEITEL-VTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVISPSGAKYTMWESGEDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAI 1025
            DDF     A+ AA++W  +RL +   E +RAP++ CA+Y  GR   + L+   +  AV  VS++E  G CF  +A  + A+A+I   E F L S +P   +LKLA  L+DH              D       L + +G  M + NV GLS+ L+PG L +   E  AEAF+ D   DL   SV++   +FWSD +    E         R R+WS+AA+VV  L+  +  + G+IC + ++ + H   D++LV G++HLL   R           +E   AC+MGL+S LA+KPEVLRV+P QS  L NA A A +QSAT T+TPL DAGLDGTG+V+QV+DSG+DE+SCFFA D+ G  V HG +F+  G+                       R  DD   G+   +   +    F     RRKI+QYI L K+ D  +  S  DSFT                 TS                         L   + +L  D               F  D   GHGT  AGS AGA    +  E  +C   E   C G C+    A+DVD +   +    DLD  CP++S D + D+  +E CLSDD  E L  +GG+A GA++++FD  Y    + + F GN LW    E   KIH+NS+GG   C L  ++ LYD +M  NPE+LLIFAAGNDG       R  CT+ SPA+ KN LAVGA++SG                                      T  ++T   G+DTVA FSS GPT D RIKP+V+APGD + SA+SDG+ D HSC+L A  GTS S P+VAGA+A++RQYF + +FFA D+ +RG C E +    C  F+PS+AT+KA++INSA+LMGGSSEP+G RGFGR+HLEAGMP+ G G   LFV ++   +I +    E    V+  A ++LR TL+WLDPP T  SA QL HDLDL VISP+G +YTMW SG  D+ NV ERVIV    +E+G WSV + A  L TD Q YSLVV GAI
Sbjct:   37 DDFNGHARAFEAAATWNGERLRHRQDE-TRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDR------LSARHGARMRMSNVEGLSIELTPGTLASRSVE--AEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDD--TEGSHGDSFT-----------------TS-------------------------LGQQYPWLPADR--------------FEYDYEAGHGTHVAGSVAGAT-LNTPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFS-DYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGL-SPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERR----RSTCTMSSPAIAKNVLAVGASTSGD-------------------------------------TRLSATPGLGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG---CEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTDVQSYSLVVNGAI 966          

BLAST of mRNA_E-siliculosus-1a_F_contig110.690.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 594 bits (1532), Expect = 6.620e-195
Identity = 401/1034 (38.78%), Postives = 540/1034 (52.22%), Query Frame = 0

Query:    4 HPDHVAAFLMLVASSSPVLGV-------DLGKIQAITASSAFDDFARSQSAYSAASSWYTDRLSYDAAEGSRAPYIACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAAGGESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVT-----RVREWSRAAAVVDSLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFP-------SETRAACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYISDYLGDPYDYDDSEYTSYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSSLEEEACYGDELPGCAGGCISAS------DVDAMLDD-STFDLDV--FCPMYSCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLSEDEYLYDTFMYENPEHLLIFAAGNDGGYEDVPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVMSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLFVIEAAIADGEITELVT------VDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVISPSGAKYTMWE-SGEDDTVNVVERVIVPVESMESGVWS 1002
            H    A F  L A+S+P  G         L  ++    ++A D F     A+  A+ W  + L     + +RAP++ C +Y +G   L  L+   +  AV  V+++   G CF VTA  + A A+  +P  + L S +P   +LK+A  +L+HG               S     L +T+G  M   NV GL++ LSPG L A   E  A  F+ +   DL S+S+++   + WSD  A   E+  T     R REWS AA +V  LA     + G+IC +  + + +  +DL+LV G++HLL  +          +E R AC+MGL+S L+ + EVLRVSP  + RL NAVA  ++QSAT TE PL DAGLDGTG+VIQV+D+GLDETSCFF DDDG +V HG +++ +                        +I+            S+SS +D                                                                              V GHGT  AGSA GA     +     C G E+PGC GGCI A       D+  ++ + S +D+D+   CPM  CD  TD     CLSDD  + L  HGG+A GA+++ FDV   G+     F GN LW   +E G KIH+ S G    C + E + LYD FMY NPE+L++ AAGN G  ED   R  CT+ SPA+GKN+LAVGATSSG    T+T  DG+L     G  D                          VDTVA FSS GPT DGRIKP+VVAPGD + SA SDG+D HSC+L A  GTS +CP+VAGA+A+VRQYF D SF+  D  +RG   +  S  +C  F+PS+ATVKA++INSA+LMGGSSEP+G RGFGR+HLE GMP+DG G   LFV +++     + EL +      VD +A ++ RATL+W+DPP ++ S  QL HDLDL V+SPSGA + MW  SGE D+ NV ERVI+  E +ESG ++
Sbjct:    5 HRTATALFFSLAAASTPWGGSANTLPYPSLSVVEGSLLTTAQDSFDGHAKAFETAAQWTGEILRSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDSPY-YGLTSISPFPSALKVAPGVLEHGVSLD---------QASQSPGRLTTTHGHLMRKGNVEGLNMELSPGTLPAHSPE--AGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEALG-----------------------EIIP-----------SSSSARDD-----------------------------------------------------------------------------VEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATD---PRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSS-GTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTED--GRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYAD--------------------------VDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDD--SGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTS--LPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTYT 879          

BLAST of mRNA_E-siliculosus-1a_F_contig110.690.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 590 bits (1520), Expect = 1.580e-191
Identity = 405/1044 (38.79%), Postives = 554/1044 (53.07%), Query Frame = 0

Query:   16 ASSSPVLGVDLGKIQAITASSAFDDFARSQSAYSAASSWYT-DRLSYDAAEGSRAPYIACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAP-LRSTYGEGMTLDNVHGLSVRLSPGVLTA-AGGESFAEAFLRDWHADLTSESVNMRRASFWSD-----SDADRSEYDVTRVREWSRAAAVVDSLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPS--------ETRAACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRV---------EDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYISDYLGDPYDYDDSEYTSYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSSLEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYSCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLSEDEYLYDTFMYENPEHLLIFAAGNDGGYEDVPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVMSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLFVIEAA----IADGEITELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVISPSGAKYTMWESGEDDTVNVVERVIVPVESME----SGVWSVVVSAKALLTDEQL-YSLVVTGAI 1025
            AS  P LG    +    TA + FD    S++A++ AS+W   DR      +G  +PY++C ++ +GRHA   LE      AV  VS+T   G CF+VTA P+ A A + + EDF    A P   +LK+A  LLDHG    P          SD SA  L +T+G  M +D+ HGL V LSPG L + +GG++ A  F+++    L+S S+++   + WSD      D   S     R REWSRAA +V+  +     T  ++CG+ ++ + H A+D++LV G++HLL   R  S        E R ACYMGL+S LA++ EVL+V+        NA A A +QSA+   TPL DAGLDG+GEVIQ++D+G++ET+CF    DG +VEHG++FD V           E             F +D  RRK++QYI L   PD G  SS    F     ++ D                                  DDL                                GHGT  A + AG+  +  +       G E+ GCAGGCI+ S   +   D   D++  CP + C G+      +CL DD  + L +HGG+A GA+I+  DV Y G   Y   AGN LW +  + G KIH+NS+G    CQL+  + LYD +MYEN EHLL+F+AGN G   D   R+ CT+ SP + KN L VG+TSSG                 L  TD    GY            +   E + +D V+ FS  G T DGRIKP+VVAPGD + SAS +G D  SC+L+A  GTS S  + +GAAA+VRQYF+D SF++ DV SRG C +  +   C  F+P A TVKAM+INSA++MGG +EP+  RGFGR+HLEAGMP+ G G+  LFV +++     +D ++     VD DAG+ELRATL+W DP  ++LS  QL +DLDL V +P G +Y MW+    DT NV ERVIVP E +     +  W V V  +     + + +SLVVTGAI
Sbjct:   21 ASQVPSLGF-FEQTLLTTAGADFD----SEAAFAFASAWQAGDR------QGRSSPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEA-AQLSSWEDFTTFGAFPA--ALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLIN-PDTGDGSSSGMPFVSADSFVSD----------------------------------DDL--------------------------------GHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSGD------QCLPDDTADTLSEHGGMAQGAKIAAIDVFY-GDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTD---RDTCTIDSPGIAKNVLTVGSTSSG--------------VTRLSTTDLDGRGY------------ADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQGFT---CDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAI 943          

BLAST of mRNA_E-siliculosus-1a_F_contig110.690.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 586 bits (1511), Expect = 5.520e-191
Identity = 406/1066 (38.09%), Postives = 544/1066 (51.03%), Query Frame = 0

Query:    4 HPDHVAAFLMLVASSSPVLGV-------DLGKIQAITASSAFDDFARSQSAYSAASSWYTDRLSYDAAEGSRAPYIACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAAGGESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVT-----RVREWSRAAAVVDSLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGK-------RFPSETRAACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPD----QGSTSSGQDSFTYISDYLGDPYDYDDSEYTSYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSSLEEEACYGDELPGCAGGCISASDVD-------AMLDDSTF--DLDVFCPMYSCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLSEDEYLYDTFMYENPEHLLIFAAGNDGGYEDVPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVMSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLFVIEAAIADGEITELVT------VDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVISPSGAKYTMWE-SGEDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGA-IYSSK 1029
            H    A FL LVA+S+P  G         LG ++    ++A + F      +  A+ W  D +     + +RAP++ C +Y +G   L+ L+   +  AV  V+++   G CF VTA  + A A+   P  + L S +P   +LK+A  +L+HG               S     L +T+G  M   NV GL++ LSPG L A   +  A  F+ +   DL S+S+++   + WSD  A   E+  T     R REWS AA +V  LA     + G+IC +  + + H  +DL+LV G++HLL  +          +E R AC+MGL+S L+++ EV RVSP  + RL NAVA  ++QSAT T+ PL  AGLDGTG+VIQV+                                                       QYI L K         ST+SG +   ++S                                                                  GF  D V  HGT TAGSA GA     +     C G E+PGC GGCI A            M ++S++  D+D  CPM  CD   +     CLSD   + L  HGG+A GA++S FD+ Y G  ++  F GN LW   +E G KIH+ S+G    C + + + LYD FMY NPE+L++ AAGN G  ED   R  CT+ SPA+GKN+LAVGATSSG      T  DG+      G +D                          VDTVA FSS GPT DGRIKP+VVAPGD V SA SDG+D HSC+L A  GTS +CP+VAGA+A+VRQYF D SF+  D  +RG C +  S  QC  F+PS+ATVKA++INSA+LMGGSSEP+G RGFGR+HLE GMP+DG G   LFV +++     + EL +      VD +A ++ RATL+W+DPP ++ S  QL HDLDL V+SPSGA + MW  SGE D+ NV ERVI+  E++ESG ++V VS   LLTD Q YSLVV GA +YSS 
Sbjct:    5 HRTATALFLSLVAASTPWGGSANSLPYPSLGVVEGSLLTTAHESFDGHAKGFETAAQWTGDIIRSKEEDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMSDNPY-YGLTSISPFPSALKVAPGVLEHGVSRD---------QASQSPGRLATTHGHLMRKGNVEGLNLELSPGTLPAHSPQ--AGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGSDEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQVI-------------------------------------------------------QYINLIKADSVSGPASSTTSGGNRVWWVS----------------------------------------------------------------PDGFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENE---PWCLSDGVEQTLADHGGMAQGAKLSFFDI-YDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIED--GRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSD--------------------------VDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDD--SGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTS--LPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENVESGTYTVRVSTNHLLTDSQNYSLVVNGAMVYSSS 903