mRNA_E-siliculosus-1a_F_contig15235.3030.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig15235.3030.1
Unique NamemRNA_E-siliculosus-1a_F_contig15235.3030.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig15235.3030.1 vs. uniprot
Match: D7G0D3_ECTSI (Similar to 3-phosphoadenosine 5-phosphosulfate synthase 2 isoform 2 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G0D3_ECTSI)

HSP 1 Score: 115 bits (288), Expect = 1.620e-28
Identity = 55/57 (96.49%), Postives = 57/57 (100.00%), Query Frame = 1 
Query:    1 GFSEADRGESVRRVGETSCLFSDSGTITIVSLVSPFRDARDSVRKRHEDQGIPFFEV 171
            GFSEADRGESVRRVGETSCLFSDSGTITIVSLVSP+RDARD+VRKRHEDQGIPFFEV
Sbjct:  262 GFSEADRGESVRRVGETSCLFSDSGTITIVSLVSPYRDARDAVRKRHEDQGIPFFEV 318
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BLAST of mRNA_E-siliculosus-1a_F_contig15235.3030.1 vs. uniprot
Match: A0A6H5KXZ4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KXZ4_9PHAE)

HSP 1 Score: 115 bits (287), Expect = 2.200e-28
Identity = 54/57 (94.74%), Postives = 57/57 (100.00%), Query Frame = 1 
Query:    1 GFSEADRGESVRRVGETSCLFSDSGTITIVSLVSPFRDARDSVRKRHEDQGIPFFEV 171
            GFSEADRGESVRRVGETSCLFSDSGTITIVSLVSP+RDARD+VRKRHEDQGIPFFE+
Sbjct:  233 GFSEADRGESVRRVGETSCLFSDSGTITIVSLVSPYRDARDAVRKRHEDQGIPFFEI 289
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BLAST of mRNA_E-siliculosus-1a_F_contig15235.3030.1 vs. uniprot
Match: A0A835YWJ1_9STRA (3-phosphoadenosine 5-phosphosulfate synthase-like protein 2 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YWJ1_9STRA)

HSP 1 Score: 93.2 bits (230), Expect = 1.120e-20
Identity = 44/56 (78.57%), Postives = 48/56 (85.71%), Query Frame = 1 
Query:    4 FSEADRGESVRRVGETSCLFSDSGTITIVSLVSPFRDARDSVRKRHEDQGIPFFEV 171
            F++ DR ESVRRVGE +CLFSDSGTITIVSLVSPFR  RD VR RHE QGIPF+EV
Sbjct:  213 FTDDDRAESVRRVGEVACLFSDSGTITIVSLVSPFRGDRDEVRARHEQQGIPFYEV 268
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BLAST of mRNA_E-siliculosus-1a_F_contig15235.3030.1 vs. uniprot
Match: A0A7S2EI26_TRICV (Adenylyl-sulfate kinase (Fragment) n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S2EI26_TRICV)

HSP 1 Score: 87.8 bits (216), Expect = 1.940e-20
Identity = 38/56 (67.86%), Postives = 48/56 (85.71%), Query Frame = 1 
Query:    4 FSEADRGESVRRVGETSCLFSDSGTITIVSLVSPFRDARDSVRKRHEDQGIPFFEV 171
            FSEADR ESVRR GE + LF+D+G IT+V L+SP+R  RD+VR+RHEDQGIPF+E+
Sbjct:  113 FSEADRAESVRRTGEIATLFADAGVITLVGLISPYRSDRDAVRRRHEDQGIPFYEI 168
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BLAST of mRNA_E-siliculosus-1a_F_contig15235.3030.1 vs. uniprot
Match: A0A7S1FVZ2_9STRA (Hypothetical protein n=1 Tax=Corethron hystrix TaxID=216773 RepID=A0A7S1FVZ2_9STRA)

HSP 1 Score: 92.0 bits (227), Expect = 2.840e-20
Identity = 41/57 (71.93%), Postives = 51/57 (89.47%), Query Frame = 1 
Query:    1 GFSEADRGESVRRVGETSCLFSDSGTITIVSLVSPFRDARDSVRKRHEDQGIPFFEV 171
            GFSE+DR ESVRRVGE + LFSD+GTIT+V L+SP+R  RD+VR+RHE+QGIPF+EV
Sbjct:  170 GFSESDRAESVRRVGEMATLFSDAGTITLVGLISPYRGDRDAVRRRHEEQGIPFYEV 226
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BLAST of mRNA_E-siliculosus-1a_F_contig15235.3030.1 vs. uniprot
Match: A0A448ZKG1_9STRA (Uncharacterized protein n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448ZKG1_9STRA)

HSP 1 Score: 90.9 bits (224), Expect = 7.250e-20
Identity = 42/57 (73.68%), Postives = 47/57 (82.46%), Query Frame = 1 
Query:    1 GFSEADRGESVRRVGETSCLFSDSGTITIVSLVSPFRDARDSVRKRHEDQGIPFFEV 171
            GFSE DR ESVRR GE + LFSDSG IT+V L+SP+R  RD VRKRHEDQGIPF+EV
Sbjct:  255 GFSEFDRAESVRRTGELATLFSDSGVITLVGLISPYRKDRDEVRKRHEDQGIPFYEV 311
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BLAST of mRNA_E-siliculosus-1a_F_contig15235.3030.1 vs. uniprot
Match: A0A7S4ADA6_9STRA (Hypothetical protein n=1 Tax=Pseudo-nitzschia australis TaxID=44445 RepID=A0A7S4ADA6_9STRA)

HSP 1 Score: 90.9 bits (224), Expect = 7.270e-20
Identity = 42/57 (73.68%), Postives = 47/57 (82.46%), Query Frame = 1 
Query:    1 GFSEADRGESVRRVGETSCLFSDSGTITIVSLVSPFRDARDSVRKRHEDQGIPFFEV 171
            GFSE DR ESVRR GE + LFSDSG IT+V L+SP+R  RD VRKRHEDQGIPF+EV
Sbjct:  678 GFSEFDRAESVRRTGELATLFSDSGVITLVGLISPYRKDRDDVRKRHEDQGIPFYEV 734
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BLAST of mRNA_E-siliculosus-1a_F_contig15235.3030.1 vs. uniprot
Match: A0A7S0TE74_9STRA (Hypothetical protein n=1 Tax=Pseudo-nitzschia delicatissima TaxID=44447 RepID=A0A7S0TE74_9STRA)

HSP 1 Score: 90.5 bits (223), Expect = 9.890e-20
Identity = 42/57 (73.68%), Postives = 47/57 (82.46%), Query Frame = 1 
Query:    1 GFSEADRGESVRRVGETSCLFSDSGTITIVSLVSPFRDARDSVRKRHEDQGIPFFEV 171
            GFSE DR ESVRR GE + LFSDSG IT+V L+SP+R  RD VRKRHEDQGIPF+EV
Sbjct:  172 GFSEWDRAESVRRTGELATLFSDSGVITLVGLISPYRKDRDEVRKRHEDQGIPFYEV 228
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BLAST of mRNA_E-siliculosus-1a_F_contig15235.3030.1 vs. uniprot
Match: A0A6U2QCL8_9STRA (Hypothetical protein n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A6U2QCL8_9STRA)

HSP 1 Score: 90.1 bits (222), Expect = 1.350e-19
Identity = 41/57 (71.93%), Postives = 49/57 (85.96%), Query Frame = 1 
Query:    1 GFSEADRGESVRRVGETSCLFSDSGTITIVSLVSPFRDARDSVRKRHEDQGIPFFEV 171
            GFS+ADR ESVRR GE + LF+DSG IT+V L+SP+R  RD+VRKRHEDQGIPF+EV
Sbjct:  240 GFSQADREESVRRTGEIATLFADSGAITLVGLISPYRADRDAVRKRHEDQGIPFYEV 296
[Close]
BLAST of mRNA_E-siliculosus-1a_F_contig15235.3030.1 vs. uniprot
Match: A0A7S1GEY9_9STRA (Adenylyl-sulfate kinase (Fragment) n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A7S1GEY9_9STRA)

HSP 1 Score: 88.2 bits (217), Expect = 3.560e-19
Identity = 39/56 (69.64%), Postives = 48/56 (85.71%), Query Frame = 1 
Query:    4 FSEADRGESVRRVGETSCLFSDSGTITIVSLVSPFRDARDSVRKRHEDQGIPFFEV 171
            FSEADR ESVRR GE + LF+DSG IT+V L+SP+R  RD+VRKRHE+QGIPF+E+
Sbjct:  245 FSEADRAESVRRTGEIATLFADSGVITLVGLISPYRGDRDAVRKRHEEQGIPFYEI 300
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig15235.3030.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G0D3_ECTSI1.620e-2896.49Similar to 3-phosphoadenosine 5-phosphosulfate syn... [more]
A0A6H5KXZ4_9PHAE2.200e-2894.74Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A835YWJ1_9STRA1.120e-2078.573-phosphoadenosine 5-phosphosulfate synthase-like ... [more]
A0A7S2EI26_TRICV1.940e-2067.86Adenylyl-sulfate kinase (Fragment) n=1 Tax=Trieres... [more]
A0A7S1FVZ2_9STRA2.840e-2071.93Hypothetical protein n=1 Tax=Corethron hystrix Tax... [more]
A0A448ZKG1_9STRA7.250e-2073.68Uncharacterized protein n=1 Tax=Pseudo-nitzschia m... [more]
A0A7S4ADA6_9STRA7.270e-2073.68Hypothetical protein n=1 Tax=Pseudo-nitzschia aust... [more]
A0A7S0TE74_9STRA9.890e-2073.68Hypothetical protein n=1 Tax=Pseudo-nitzschia deli... [more]
A0A6U2QCL8_9STRA1.350e-1971.93Hypothetical protein n=1 Tax=Leptocylindrus danicu... [more]
A0A7S1GEY9_9STRA3.560e-1969.64Adenylyl-sulfate kinase (Fragment) n=1 Tax=Skeleto... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig15235contigE-siliculosus-1a_F_contig15235:43..693 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start0
Stop1
Cds size186
Model size186
Exons2
Hectar predicted targeting categoryother localisation
BRITEko00000,ko00001,ko00002,ko01000
Best eggNOG OGNA|NA|NA
Best tax levelEukaryota
COG Functional cat.P
EC2.7.1.25,2.7.7.4,3.1.3.62,3.1.3.80,3.6.1.1
Ec32 orthologEc-19_000180.1
Ec32 ortholog descriptionAdenylylsulfate kinase
EggNOG OGsCOG0221@1,COG0529@1,KOG1626@2759,KOG4238@2759
EggNOG free text desc.3'-phosphoadenosine 5'-phosphosulfate biosynthetic process
GOsGO:0000103,GO:0001501,GO:0003674,GO:0003824,GO:0004020,GO:0004779,GO:0004781,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005829,GO:0006082,GO:0006139,GO:0006163,GO:0006164,GO:0006725,GO:0006753,GO:0006790,GO:0006793,GO:0006796,GO:0006807,GO:0006950,GO:0007275,GO:0007596,GO:0007599,GO:0008150,GO:0008152,GO:0009058,GO:0009117,GO:0009150,GO:0009152,GO:0009165,GO:0009259,GO:0009260,GO:0009336,GO:0009611,GO:0009987,GO:0016301,GO:0016310,GO:0016740,GO:0016772,GO:0016773,GO:0016779,GO:0018130,GO:0019438,GO:0019637,GO:0019693,GO:0032501,GO:0032502,GO:0032991,GO:0033865,GO:0033866,GO:0033875,GO:0034030,GO:0034032,GO:0034033,GO:0034035,GO:0034036,GO:0034641,GO:0034654,GO:0042060,GO:0042802,GO:0042803,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0044237,GO:0044238,GO:0044249,GO:0044271,GO:0044272,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0046390,GO:0046483,GO:0046983,GO:0048513,GO:0048731,GO:0048856,GO:0050427,GO:0050428,GO:0050817,GO:0050878,GO:0050896,GO:0055086,GO:0060348,GO:0061695,GO:0065007,GO:0065008,GO:0070566,GO:0071704,GO:0072521,GO:0072522,GO:0090407,GO:1901135,GO:1901137,GO:1901293,GO:1901360,GO:1901362,GO:1901564,GO:1901566,GO:1901576,GO:1902494,GO:1902503,GO:1990234
KEGG ModuleM00176,M00596
KEGG Pathwayko00010,ko00190,ko00230,ko00261,ko00450,ko00562,ko00920,ko01100,ko01120,ko01130,map00010,map00190,map00230,map00261,map00450,map00562,map00920,map01100,map01120,map01130
KEGG ReactionR00509,R00529,R03434,R04928,R04929,R09532
KEGG koko:K00860,ko:K00958,ko:K01507,ko:K03103,ko:K13811
KEGG rclassRC00002,RC00017,RC00078,RC02809,RC02889
Preferred namePAPSS2
Seed eggNOG ortholog2880.D7G0D3
Seed ortholog evalue1.9e-23
Seed ortholog score114.4
Taxonomic scopeEukaryota
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928032.935329-CDS-E-siliculosus-1a_F_contig15235:42..1181622928032.935329-CDS-E-siliculosus-1a_F_contig15235:42..118Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig15235 43..118 -
1696318780.996685-CDS-E-siliculosus-1a_F_contig15235:42..1181696318780.996685-CDS-E-siliculosus-1a_F_contig15235:42..118Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig15235 43..118 -
1622928032.9491343-CDS-E-siliculosus-1a_F_contig15235:583..6931622928032.9491343-CDS-E-siliculosus-1a_F_contig15235:583..693Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig15235 584..693 -
1696318781.0142102-CDS-E-siliculosus-1a_F_contig15235:583..6931696318781.0142102-CDS-E-siliculosus-1a_F_contig15235:583..693Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig15235 584..693 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig15235.3030.1prot_E-siliculosus-1a_F_contig15235.3030.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig15235 43..693 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig15235.3030.1

>prot_E-siliculosus-1a_F_contig15235.3030.1 ID=prot_E-siliculosus-1a_F_contig15235.3030.1|Name=mRNA_E-siliculosus-1a_F_contig15235.3030.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=62bp
GFSEADRGESVRRVGETSCLFSDSGTITIVSLVSPFRDARDSVRKRHEDQ
GIPFFEVQSYI*
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mRNA from alignment at E-siliculosus-1a_F_contig15235:43..693-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig15235.3030.1 ID=mRNA_E-siliculosus-1a_F_contig15235.3030.1|Name=mRNA_E-siliculosus-1a_F_contig15235.3030.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=651bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig15235:43..693- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
GGCTTCTCGGAGGCGGACCGCGGAGAGTCGGTCCGACGCGTGGGCGAGAC
CTCCTGCCTCTTCTCCGACTCGGGGACGATCACCATCGTGTCTCTCGTCT
CTCCTTTCCGGTAAGCCTGCTCGCAAACAGCGTGGGGTCGACGCTAGTCT
GTGCAGTACCGTATATGACTTAAGATAACACACCCCCTTGACCGATTTTC
AAAGCTCGATTTCTCAACAGCAGGAAGACTTCCAGCGCCGAAAACGGCGG
CGTTGGAACCGTCTCGTTGAGAGCTTTTCGAAAATGTATTGTTTCGTTTA
TGTATCCTCTTCGTCGTCGAGTAATCGAGCTTTGAAAATCGGGCCAGGGG
GTGTCTTATCTTGTGTCATATACGATATGCTCAGGCGTTTTGGTTGTTGT
GGCGGTGTTGGGAATCGTGTTGGCACGTGTGGTGATGTCACCGCCTCTGT
TTTTAGTCACAGGTGCTCCCTCCGAGGTTACCACTGGTTATCCCCTAGTC
TTCGCTCACACTTCGCACCCTGTTTCTGTGCCTCATCTGTCCTTGGTTGT
CCCTGGCGTTCCTTGTGTGCGACAGAGATGCCCGCGATTCTGTCCGCAAG
AGGCACGAGGACCAGGGCATCCCCTTCTTCGAGGTACAGAGTTACATTTG
A
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig15235:43..693-

>mRNA_E-siliculosus-1a_F_contig15235.3030.1 ID=mRNA_E-siliculosus-1a_F_contig15235.3030.1|Name=mRNA_E-siliculosus-1a_F_contig15235.3030.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=372bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig15235:43..693- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
GGCTTCTCGGAGGCGGACCGCGGAGAGTCGGTCCGACGCGTGGGCGAGAC
CTCCTGCCTCTTCTCCGACTCGGGGACGATCACCATCGTGTCTCTCGTCT
CTCCTTTCCGGGCTTCTCGGAGGCGGACCGCGGAGAGTCGGTCCGACGCG
TGGGCGAGACCTCCTGCCTCTTCTCCGACTCGGGGACGATCACCATCGTG
TCTCTCGTCTCTCCTTTCCGAGATGCCCGCGATTCTGTCCGCAAGAGGCA
CGAGGACCAGGGCATCCCCTTCTTCGAGGTACAGAGTTACATTTGAAGAT
GCCCGCGATTCTGTCCGCAAGAGGCACGAGGACCAGGGCATCCCCTTCTT
CGAGGTACAGAGTTACATTTGA
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