mRNA_E-siliculosus-1a_F_contig138.2257.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig138.2257.1
Unique NamemRNA_E-siliculosus-1a_F_contig138.2257.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig138.2257.1 vs. uniprot
Match: D7G1I8_ECTSI (EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G1I8_ECTSI)

HSP 1 Score: 130 bits (327), Expect = 9.640e-32
Identity = 67/84 (79.76%), Postives = 69/84 (82.14%), Query Frame = 1 
Query:   70 PVEETPL-------GLVDHYLGENRVIGRDITEEQAHNLNAVHRLLLRVEAVHAASWLWVSDSPDIAHTAEVTNRPMVTPSARN 300
            P   TPL       GLVDHYLGENRVIGRDITEEQAH LNAVHRLLLRVEAVHAASWLWVSD+PDIAHTAEVT R  VTPS R+
Sbjct:  285 PFGRTPLLCSIEDEGLVDHYLGENRVIGRDITEEQAHKLNAVHRLLLRVEAVHAASWLWVSDAPDIAHTAEVTKRTKVTPSPRS 368
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BLAST of mRNA_E-siliculosus-1a_F_contig138.2257.1 vs. uniprot
Match: A0A6H5JWZ8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JWZ8_9PHAE)

HSP 1 Score: 120 bits (300), Expect = 3.350e-28
Identity = 66/100 (66.00%), Postives = 72/100 (72.00%), Query Frame = 1 
Query:    1 VRMIIDAGADTTSAVRVSKLGFLPVEETPLGLVDHYLGENRVIGRDITEEQAHNLNAVHRLLLRVEAVHAASWLWVSDSPDIAHTAEVTNRPMVTPSARN 300
            VRMI+DA ADTTSAVRV KLG LPV+ETPLGL                   AH L+AVHRLLLRVEA+HAASWLWVSD+PDIAHTAEVT R  V P AR+
Sbjct:  226 VRMIVDAVADTTSAVRVLKLGLLPVKETPLGL-------------------AHKLSAVHRLLLRVEALHAASWLWVSDAPDIAHTAEVTKRTKVIPPARS 306
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BLAST of mRNA_E-siliculosus-1a_F_contig138.2257.1 vs. uniprot
Match: D8LSC5_ECTSI (EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSC5_ECTSI)

HSP 1 Score: 96.7 bits (239), Expect = 2.670e-20
Identity = 49/88 (55.68%), Postives = 60/88 (68.18%), Query Frame = 1 
Query:    1 VRMIIDAGADTTSAVRVSKL-GFLPVEETPLGLVDHYLGENRVIGRDITEEQAHNLNAVHRLLLRVEAVHAASWLWVSDSPDIAHTAE 261
            VR+++DAGADTTS VRV+ L G +   +TPL +    L E  V G+  TEEQ H L A+ RLLLRV+AVHA SWLW SD+P I H  E
Sbjct:   81 VRLLVDAGADTTSTVRVTNLNGGVEYNDTPLAVTTSLLREKVVDGKPATEEQLHRLQAIRRLLLRVDAVHAVSWLWPSDAPSIVHAVE 168
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BLAST of mRNA_E-siliculosus-1a_F_contig138.2257.1 vs. uniprot
Match: D7G1U9_ECTSI (EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G1U9_ECTSI)

HSP 1 Score: 80.5 bits (197), Expect = 1.550e-13
Identity = 41/84 (48.81%), Postives = 54/84 (64.29%), Query Frame = 1 
Query:    4 RMIIDAGADTTSAVRVSKLG-FLPVEETPLGLVDHYLGENRVIGRDITEEQAHNLNAVHRLLLRVEAVHAASWLWVSDSPDIAH 252
            R +ID GADT S V V++ G +    +TPLG     L +N+V G++ TEEQ  +L A+HRLL ++EAVHA SWLW S    I H
Sbjct:  300 RFLIDDGADTKSTVLVTRSGEWTTSRDTPLGFATRSLRQNKVGGKNATEEQLRSLKAIHRLLSQIEAVHAVSWLWRSSDTPIGH 383
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BLAST of mRNA_E-siliculosus-1a_F_contig138.2257.1 vs. uniprot
Match: A0A6H5L1C9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L1C9_9PHAE)

HSP 1 Score: 79.3 bits (194), Expect = 2.330e-13
Identity = 49/115 (42.61%), Postives = 64/115 (55.65%), Query Frame = 1 
Query:    1 VRMIIDAGADTTSAVRVS-KLGFLPVEETPLGLVDHYLGENRVIGRDITEEQAHNLNAVHRLLLRVEAVHAASWLWVSDSPDIAHTAEVTNRPMVTPSARNATPFCRPCSGIHRR 342
            VR+++DAGADTTS VR + + G LPV  TP+ +  + L +    G  I +E  H L  + RLLLRVEAVHA SWLW +  P +   A  +  P  T +     P  RP   I RR
Sbjct:  223 VRLLVDAGADTTSIVRFTIQPGTLPVVCTPMSVDTNALHQKAAYGTAIPDELQHRLGLIRRLLLRVEAVHATSWLWQNGIPRVGLAARGSPNPK-TKATSTTGPQLRPMLPILRR 336
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BLAST of mRNA_E-siliculosus-1a_F_contig138.2257.1 vs. uniprot
Match: D7FVU0_ECTSI (EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FVU0_ECTSI)

HSP 1 Score: 78.2 bits (191), Expect = 7.850e-13
Identity = 44/89 (49.44%), Postives = 54/89 (60.67%), Query Frame = 1 
Query:    1 VRMIIDAGADTTSAVR-VSKLGFLPVEETPLGLVDHYLGENRVI-GRDITEEQAHNLNAVHRLLLRVEAVHAASWLWVSDSPDIAHTAE 261
            VR++I+AGADTTS +R  +  G +    TPL L   YL E  +  G   TEE    L A+ RLLLR EAVHA SWLW SD+  + H AE
Sbjct:  289 VRLLINAGADTTSVIRGTNSEGEVQCNGTPLALTTSYLWEKILDNGEPATEEHLQRLEAIRRLLLRAEAVHAVSWLWPSDASFVGHAAE 377
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BLAST of mRNA_E-siliculosus-1a_F_contig138.2257.1 vs. uniprot
Match: D7FSU4_ECTSI (EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FSU4_ECTSI)

HSP 1 Score: 75.9 bits (185), Expect = 2.510e-12
Identity = 48/95 (50.53%), Postives = 58/95 (61.05%), Query Frame = 1 
Query:    4 RMIIDAGADTTSAVRVSKLGFLPVEETPLGLVDHYLGENRVIGRDITEEQAHNLNAVHRLLLRVEAVHAASWLWVSDSPDIAH-TAEVTNRPMVT 285
            +++++AGADTTSAV VSK  F     TPLGL    L         +TEEQ   L A+ RLLLRVEAVHA SWLW +D   I H TA  T+R + T
Sbjct:  169 QLLVEAGADTTSAVPVSK-HFGKDSVTPLGLTMRLLDGRSTY--PLTEEQLKKLKAIRRLLLRVEAVHAVSWLWSTDISTITHSTAHGTDRTIAT 260
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BLAST of mRNA_E-siliculosus-1a_F_contig138.2257.1 vs. uniprot
Match: A0A6H5KJA9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KJA9_9PHAE)

HSP 1 Score: 76.6 bits (187), Expect = 3.170e-12
Identity = 46/89 (51.69%), Postives = 59/89 (66.29%), Query Frame = 1 
Query:    1 VRMIIDAGADTTSAVRV-SKLGFLPVEETPLGLVDHYLGEN-RVIGRD-ITEEQAHNLNAVHRLLLRVEAVHAASWLWVSDSPDIAHTA 258
            VR++++AGADTTS V V ++LG   V  TPLGL  + + +  +V+GR   + EQ H L A+ RLLLRVEAVHA SWLW SD   I   A
Sbjct:  305 VRLLVEAGADTTSPVAVRNQLGGDDVSITPLGLTMNPVRDKVKVVGRGPASPEQLHKLEAIRRLLLRVEAVHAVSWLWPSDISTITRAA 393
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BLAST of mRNA_E-siliculosus-1a_F_contig138.2257.1 vs. uniprot
Match: D7FT11_ECTSI (EsV-1-1 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FT11_ECTSI)

HSP 1 Score: 74.3 bits (181), Expect = 2.240e-11
Identity = 44/94 (46.81%), Postives = 55/94 (58.51%), Query Frame = 1 
Query:    1 VRMIIDAGADTTSAVRVSKL-GFLPVEETPLGLVDHYLGENRVI-GRDITEEQAHNLNAVHRLLLRVEAVHAASWLWVSDSPDIAHTAEVTNRP 276
            VRM++DAGADTTS V+V  L G +   +TPL      L +  V  G+  T+E    L A+ RLLLRV+AVHA SWLW S +P I    E    P
Sbjct:  401 VRMLVDAGADTTSPVQVMNLKGGVVFNDTPLAFTKLRLRKKIVADGKPATKEHLQRLEAICRLLLRVDAVHAVSWLWPSGAPFIGRATETDPEP 494
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BLAST of mRNA_E-siliculosus-1a_F_contig138.2257.1 vs. uniprot
Match: D7FVK3_ECTSI (EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FVK3_ECTSI)

HSP 1 Score: 71.6 bits (174), Expect = 1.600e-10
Identity = 46/113 (40.71%), Postives = 57/113 (50.44%), Query Frame = 1 
Query:    1 VRMIIDAGADTTSAVRVS-KLGFLPVEETPLGLVDHYLGENRVIGRDITEEQAHNLNAVHRLLLRVEAVHAASWLWVSDSPDIAHTA------EVTNRPMVTPSARNATPFCR 318
            VR+++DAGADTTS VR + + G LPV  TPL L    L       + I +E    L  + RLLLRVEAVHA SWLW +  P +   A      E  +     P  R   P  R
Sbjct:  307 VRLLVDAGADTTSIVRFTIQPGTLPVVCTPLSLATEALRIKIAYRKAIPDELLRRLELIRRLLLRVEAVHATSWLWQNGVPRVGLPAGGTAATETESSSTTAPQLRTVLPILR 419
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig138.2257.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 18
Match NameE-valueIdentityDescription
D7G1I8_ECTSI9.640e-3279.76EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=288... [more]
A0A6H5JWZ8_9PHAE3.350e-2866.00Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LSC5_ECTSI2.670e-2055.68EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=288... [more]
D7G1U9_ECTSI1.550e-1348.81EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=288... [more]
A0A6H5L1C9_9PHAE2.330e-1342.61Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FVU0_ECTSI7.850e-1349.44EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=288... [more]
D7FSU4_ECTSI2.510e-1250.53EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=288... [more]
A0A6H5KJA9_9PHAE3.170e-1251.69Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FT11_ECTSI2.240e-1146.81EsV-1-1 n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
D7FVK3_ECTSI1.600e-1040.71EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=288... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig138contigE-siliculosus-1a_F_contig138:3721..5357 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start1
Stop1
Cds size351
Model size827
Exons3
Ec32 orthologEc-07_001190.1
Ec32 ortholog descriptionEsV-1-199-related ankyrin repeat protein
Hectar predicted targeting categoryother localisation
Best eggNOG OGNA|NA|NA
Best tax levelEukaryota
COG Functional cat.G
EggNOG OGsCOG0666@1,KOG4177@2759
EggNOG free text desc.response to abiotic stimulus
Seed eggNOG ortholog2880.D7G1I8
Seed ortholog evalue3.7e-28
Seed ortholog score131.0
Taxonomic scopeEukaryota
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig138.2257.1prot_E-siliculosus-1a_F_contig138.2257.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig138 4191..5351 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927892.5421023-UTR-E-siliculosus-1a_F_contig138:3720..41901622927892.5421023-UTR-E-siliculosus-1a_F_contig138:3720..4190Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig138 3721..4190 -
1696318725.9809995-UTR-E-siliculosus-1a_F_contig138:3720..41901696318725.9809995-UTR-E-siliculosus-1a_F_contig138:3720..4190Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig138 3721..4190 -
1622927892.6361759-UTR-E-siliculosus-1a_F_contig138:5351..53571622927892.6361759-UTR-E-siliculosus-1a_F_contig138:5351..5357Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig138 5352..5357 -
1696318726.02954-UTR-E-siliculosus-1a_F_contig138:5351..53571696318726.02954-UTR-E-siliculosus-1a_F_contig138:5351..5357Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig138 5352..5357 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927892.5709167-CDS-E-siliculosus-1a_F_contig138:4190..42191622927892.5709167-CDS-E-siliculosus-1a_F_contig138:4190..4219Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig138 4191..4219 -
1696318725.9928532-CDS-E-siliculosus-1a_F_contig138:4190..42191696318725.9928532-CDS-E-siliculosus-1a_F_contig138:4190..4219Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig138 4191..4219 -
1622927892.5915415-CDS-E-siliculosus-1a_F_contig138:4995..50251622927892.5915415-CDS-E-siliculosus-1a_F_contig138:4995..5025Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig138 4996..5025 -
1696318726.0052466-CDS-E-siliculosus-1a_F_contig138:4995..50251696318726.0052466-CDS-E-siliculosus-1a_F_contig138:4995..5025Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig138 4996..5025 -
1622927892.6161401-CDS-E-siliculosus-1a_F_contig138:5059..53511622927892.6161401-CDS-E-siliculosus-1a_F_contig138:5059..5351Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig138 5060..5351 -
1696318726.0214796-CDS-E-siliculosus-1a_F_contig138:5059..53511696318726.0214796-CDS-E-siliculosus-1a_F_contig138:5059..5351Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig138 5060..5351 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig138.2257.1

>prot_E-siliculosus-1a_F_contig138.2257.1 ID=prot_E-siliculosus-1a_F_contig138.2257.1|Name=mRNA_E-siliculosus-1a_F_contig138.2257.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=117bp
MIIDAGADTTSAVRVSKLGFLPVEETPLGLVDHYLGENRVIGRDITEEQA
HNLNAVHRLLLRVEAVHAASWLWVSDSPDIAHTAEVTNRPMVTPSARNAT
PFCRPCSGIHRRPDLP*
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mRNA from alignment at E-siliculosus-1a_F_contig138:3721..5357-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig138.2257.1 ID=mRNA_E-siliculosus-1a_F_contig138.2257.1|Name=mRNA_E-siliculosus-1a_F_contig138.2257.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=1637bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig138:3721..5357- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
GTGCGGATGATTATTGACGCCGGCGCGGACACGACATCGGCAGTTCGAGT
GTCGAAACTTGGGTTTTTACCTGTGGAAGAAACCCCCCTAGGGCTCGTGG
ACCACTACCTCGGCGAGAACAGGGTCATAGGAAGGGACATCACAGAAGAG
CAGGCCCACAATCTGAACGCCGTCCACCGTTTGCTGCTGAGGGTTGAGGC
AGTTCACGCAGCCTCTTGGCTGTGGGTAAGCGATTCCCCAGATATCGCTC
ATACTGCAGAGGTTACAAATAGGCCCATGGTGACTCCGTCCGCGCGCAGC
AGGATGCTGCCGGTTCTAGGATTGAGCACAAGACGCAACGCCCTTCTGCC
GGCCGTGCTCAGGTGGGCAGGATGTATTGGTTCAGGGCCGGAACTATTGC
GCTTATCTCTTGGGTATGTTGAATAGATCTAGTGGACAGTAGTTCATCGA
AAGGTTGATCGGCACTCAAACAGTAGTCAGGAACCTATCGATGTGTCTAG
ATCAATCTTGCGGGGGTTCGAACTGTGAAGTTGTCCGGGGGGCGTCCATC
GCGTAGAATTCGACAGATAAGCACCTCGAAAAGGAGTAATCATATCCATA
CTAGGAATCCACCAGATGGCGGTAGCAGTCCCTTGTCACGTTGAACTTCC
TTGGTTTACACGTGGTTTTCTGCTGCGACAATCACCATGAGAATACACTG
GCTGTTTGTCGCGGTGCCAACTTGACGTGTGACATGGTACGTTTGCGGCA
AGGTACTATGGCCCGTTTCTCTACATCATTGATACCGCTATAGTCCTGTT
TGTATTGATGTAGACTGGCTGCCATCCTGCCGTCTCGAATCCCGTTCTGT
AAATATGCGATTTTTGGTGGGTGTGTCGACGAAATCGTAAATGTCTTGTT
TTACCTCTAGAACCTATTTTGGTTGCTACTGCCACACCAAGGTTCGAGCC
ATGTTTGACCTGCCGACGTCCACACCGACCGGCGGTCAAGTCGATCTGAT
TATTGACCTTGGCAGGCGTCCGCATCATTGGAGATGTTTGAAGGTACTGA
GCAGAGCCCGCAAGGGTTTTATTGTTGAAGAAACAACAGTCAACAGTAGC
GCCATTGTTCTTGGCGCTCTCACATGTGTCTTTTACAGGTATTCATCGAA
GACCTGACCTCCCTTGAGCGGTGAGAGCCGCATTTTCGCCAGTCTACCTG
CTTGCTCCATGGACTGGCGCTTCGTGCATTGCTCCCCCTGGGCATGCACG
GGAGATGGGTTGCCAGTTAACGAGGCACGACATTATGCGGTGGATGGCAC
ATCCAGCACACGAGAGTTGCGAAGCGGTTCCAACGGTGGTGCGTGCGCCA
CAATGGCGGCATGTGCAGTGGCTCGATGTGGTGGTGTTTTTTGTCGATGT
TCTCAGTGTTTCGTTTTTTTGTTTGGTGCAGATGATTAACTGGACGCACA
GGGCGCTCGCTACCCATGCAGGTTGCGTTTCAATGTACCAGTCTTAGGCC
TCGCTTGATTGAATTTCCCTCCGTTACCCACCAAGGTGGCCATTGCTGTT
AATAATATATCCCAAAAAAGTGTTGTTAACGGTATAGTTATGATTTTTCG
ATTCCGAGGACTTCACTTCACTGGCACGACACTGCGT
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig138:3721..5357-

>mRNA_E-siliculosus-1a_F_contig138.2257.1 ID=mRNA_E-siliculosus-1a_F_contig138.2257.1|Name=mRNA_E-siliculosus-1a_F_contig138.2257.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=702bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig138:3721..5357- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATGATTATTGACGCCGGCGCGGACACGACATCGGCAGTTCGAGTGTCGAA
ACTTGGGTTTTTACCTGTGGAAGAAACCCCCCTAGGGCTCGTGGACCACT
ACCTCGGCGAGAACAGGGTCATAGGAAGGGACATCACAGAAGAGCAGGCC
CACAATCTGAACGCCGTCCACCGTTTGCTGCTGAGGGTTGAGGCAGTTCA
CGCAGCCTCTTGGCTGTGGGTAAGCGATTCCCCAGATATCGCTCATACTG
CAGAGGTTACAAATAGGCCCATGGTGACTCCGTCCGCGCGCAATGATTAT
TGACGCCGGCGCGGACACGACATCGGCAGTTCGAGTGTCGAAACTTGGGT
TTTTACCTGTGGAAGAAACCCCCCTAGGGCTCGTGGACCACTACCTCGGC
GAGAACAGGGTCATAGGAAGGGACATCACAGAAGAGCAGGCCCACAATCT
GAACGCCGTCCACCGTTTGCTGCTGAGGGTTGAGGCAGTTCACGCAGCCT
CTTGGCTGTGGGTAAGCGATTCCCCAGATATCGCTCATACTGCAGAGGTT
ACAAATAGGCCCATGGTGACTCCGTCCGCGCGCAACGCAACGCCCTTCTG
CCGGCCGTGCTCAGACGCAACGCCCTTCTGCCGGCCGTGCTCAGGTATTC
ATCGAAGACCTGACCTCCCTTGAGTATTCATCGAAGACCTGACCTCCCTT
GA
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