mRNA_E-siliculosus-1a_F_contig1305.1860.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig1305.1860.1
Unique NamemRNA_E-siliculosus-1a_F_contig1305.1860.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1860.1 vs. uniprot
Match: A0A6H5KNI6_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KNI6_9PHAE)

HSP 1 Score: 115 bits (289), Expect = 7.600e-28
Identity = 60/91 (65.93%), Postives = 68/91 (74.73%), Query Frame = 1 
Query:   61 MTTEGRSGCLDAFVVALNQACNG---RWYASTLELQLVSLALCGACRSVPTLHMRVNRQTPPRLWPVCTPHTTPNKSTPEVRISSCVPAIE 324
            MTT+G S CLDAFVVALNQAC+G   RWY STLELQLVSL +CGACR V TLH+RV  QTPP LW     HTT N  +P+V ISS VP ++
Sbjct:    1 MTTKGNSKCLDAFVVALNQACDGQEKRWYVSTLELQLVSLEVCGACRRVQTLHVRVKPQTPPSLWFPSQNHTTTNNRSPKVCISSRVPVVQ 91
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1860.1 vs. uniprot
Match: A0A6H5KLV1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KLV1_9PHAE)

HSP 1 Score: 111 bits (278), Expect = 4.710e-27
Identity = 56/87 (64.37%), Postives = 64/87 (73.56%), Query Frame = 1 
Query:   73 GRSGCLDAFVVALNQACNG---RWYASTLELQLVSLALCGACRSVPTLHMRVNRQTPPRLWPVCTPHTTPNKSTPEVRISSCVPAIE 324
            GRS CL AF+VALNQ CNG   RWYASTLELQLVSL  CGACR VPTLH+RV+RQTP  LW  C  H T  K + E  +S+ VPA++
Sbjct:   13 GRSECLRAFIVALNQTCNGQEKRWYASTLELQLVSLEFCGACRRVPTLHVRVDRQTPRSLWSPCKSHATTKKRSREACVSNRVPAVQ 99
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1860.1 vs. uniprot
Match: D7FTK8_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FTK8_ECTSI)

HSP 1 Score: 100 bits (250), Expect = 4.700e-24
Identity = 55/94 (58.51%), Postives = 64/94 (68.09%), Query Frame = 1 
Query:   55 VAMT-TEGRSGCLDAFVVALNQACNG---RWYASTLELQLVSLALCGACRSVPTLHMRVNRQTPPRLWPVCTPHTTPNKSTPEVRISSCVPAIE 324
            VAM  TEG    LD  +VALNQAC+G   RWY STLELQLVSL LCGACRS   LH+ V   TPP LW   T  TTP++ +PEV  S+ VPA++
Sbjct:   78 VAMANTEGGGSSLDTLIVALNQACDGQEKRWYVSTLELQLVSLELCGACRSAQLLHVFVREDTPPTLWSSRTSRTTPSQRSPEVCTSNRVPAVQ 171
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1860.1 vs. uniprot
Match: D7G0Z9_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G0Z9_ECTSI)

HSP 1 Score: 97.4 bits (241), Expect = 6.680e-24
Identity = 50/69 (72.46%), Postives = 55/69 (79.71%), Query Frame = 1 
Query:   76 RSGCLDAFVVALNQACNG---RWYASTLELQLVSLALCGACRSVPTLHMRVNRQTPPRLWPVCTPHTTP 273
            RS CLDAFVVALNQ C+G   RWYASTLELQLVSL  CGACR VPTLH+RV+R+TP  LW   +P TTP
Sbjct:   38 RSKCLDAFVVALNQTCDGQEKRWYASTLELQLVSLEFCGACRHVPTLHVRVDRRTPRSLW---SPRTTP 103
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1860.1 vs. uniprot
Match: D7G3G0_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G3G0_ECTSI)

HSP 1 Score: 94.7 bits (234), Expect = 1.670e-23
Identity = 48/68 (70.59%), Postives = 52/68 (76.47%), Query Frame = 1 
Query:   61 MTTEGRSGCLDAFVVALNQACNGR---WYASTLELQLVSLALCGACRSVPTLHMRVNRQTPPRLWPVC 255
            M  EGR  CLDAF+VALNQ C+GR   WYASTLELQLVSL  CGACR  PTLH+RV+RQTP R  P C
Sbjct:    1 MNLEGRRKCLDAFIVALNQTCDGRENRWYASTLELQLVSLKFCGACRRFPTLHVRVDRQTP-RKKPTC 67
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1860.1 vs. uniprot
Match: D7G7F9_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G7F9_ECTSI)

HSP 1 Score: 88.6 bits (218), Expect = 4.610e-20
Identity = 47/90 (52.22%), Postives = 56/90 (62.22%), Query Frame = 1 
Query:   61 MTTEGRSGCLDAFVVALNQACNG---RWYASTLELQLVSLALCGACRSVPTLHMRVNRQTPPRLWPVCTPHTTPNKSTPEVRISSCVPAI 321
            M TEG +   DAF +AL+QAC G   RWY ST ELQL SL +C ACRS PTLH+RV   TP  LW   T    PN  + +VR+ S VP +
Sbjct:    1 MPTEGYTRSFDAFAIALSQACEGGEKRWYTSTTELQLGSLDICRACRSTPTLHVRVTGGTPTSLWSPPTVQALPNTQSSKVRVVSRVPVV 90
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1860.1 vs. uniprot
Match: A0A6H5LIJ3_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5LIJ3_9PHAE)

HSP 1 Score: 92.4 bits (228), Expect = 1.300e-19
Identity = 50/92 (54.35%), Postives = 60/92 (65.22%), Query Frame = 1 
Query:   55 VAMTTEGRSGCLDAFVVALNQACNG---RWYASTLELQLVSLALCGACRSVPTLHMRVNRQTPPRLWPVCTPHTTPNKSTPEVRISSCVPAI 321
            +A   E R+ CLDAFV+AL+QAC G   RWYASTLELQL SL LC ACR+   LH+ V+ QTP  LW   T  T PN  +  + ISS VP +
Sbjct:    1 MARVIEWRTRCLDAFVIALSQACEGGEKRWYASTLELQLTSLDLCRACRTTSILHVHVDDQTPRSLWSSRTAQTRPNTRSWNLHISSRVPVV 92
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1860.1 vs. uniprot
Match: A0A6H5JSE5_9PHAE (Reverse transcriptase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JSE5_9PHAE)

HSP 1 Score: 92.0 bits (227), Expect = 1.860e-19
Identity = 46/72 (63.89%), Postives = 53/72 (73.61%), Query Frame = 1 
Query:   73 GRSGCLDAFVVALNQACNGR---WYASTLELQLVSLALCGACRSVPTLHMRVNRQTPPRLWPVCTPHTTPNK 279
            G+S CLDAF+V +NQA +GR   WYASTLELQLVSLA CGACRS   LH+ V+R  PP LW     +TTPNK
Sbjct:  971 GKSKCLDAFIVTMNQAADGRKQQWYASTLELQLVSLAFCGACRSAKMLHLHVDRGKPPSLWFPHKSYTTPNK 1042
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1860.1 vs. uniprot
Match: A0A6H5K8K9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K8K9_9PHAE)

HSP 1 Score: 87.4 bits (215), Expect = 7.300e-18
Identity = 48/87 (55.17%), Postives = 58/87 (66.67%), Query Frame = 1 
Query:   73 GRSGCLDAFVVALNQACNGR---WYASTLELQLVSLALCGACRSVPTLHMRVNRQTPPRLWPVCTPHTTPNKSTPEVRISSCVPAIE 324
            GRS CLDAF+VAL QA +GR   WY STLELQLVSL  CGACRS  +  ++++RQTP  LW     H T  K + E  I S VPA++
Sbjct:   25 GRSECLDAFIVALKQAGDGRAKRWYVSTLELQLVSLEFCGACRSALSFDVQIHRQTPHSLWSPRKSHGTLKKRSREACIPSRVPAVQ 111
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BLAST of mRNA_E-siliculosus-1a_F_contig1305.1860.1 vs. uniprot
Match: A0A6H5JHB0_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JHB0_9PHAE)

HSP 1 Score: 80.5 bits (197), Expect = 2.020e-15
Identity = 45/86 (52.33%), Postives = 52/86 (60.47%), Query Frame = 1 
Query:   73 GRSGCLDAFVVALNQACNGR---WYASTLELQLVSLALCGACRSVPTLHMRVNRQTPPRLWPVCTPHTTPNKSTPEVRISSCVPAI 321
            G S CLDAF + L+QA  GR   WY STL+LQ V LALC ACRSVPT H+R++ QTP  LW             P VR SS VP +
Sbjct:  139 GGSRCLDAFAITLHQAYGGRRGHWYKSTLQLQCVCLALCSACRSVPTWHVRIDDQTPHSLWD------------PNVRTSSRVPVV 212
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig1305.1860.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 20
Match NameE-valueIdentityDescription
A0A6H5KNI6_9PHAE7.600e-2865.93Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5KLV1_9PHAE4.710e-2764.37Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FTK8_ECTSI4.700e-2458.51Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7G0Z9_ECTSI6.680e-2472.46Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7G3G0_ECTSI1.670e-2370.59Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7G7F9_ECTSI4.610e-2052.22Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5LIJ3_9PHAE1.300e-1954.35Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JSE5_9PHAE1.860e-1963.89Reverse transcriptase domain-containing protein n=... [more]
A0A6H5K8K9_9PHAE7.300e-1855.17Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JHB0_9PHAE2.020e-1552.33Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig1305contigE-siliculosus-1a_F_contig1305:11096..13833 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start1
Stop0
Cds size264
Model size325
Exons2
Hectar predicted targeting categoryother localisation
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig1305.1860.1prot_E-siliculosus-1a_F_contig1305.1860.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig1305 11156..13833 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927827.1332207-UTR-E-siliculosus-1a_F_contig1305:11095..111551622927827.1332207-UTR-E-siliculosus-1a_F_contig1305:11095..11155Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig1305 11096..11155 +
1696318697.0100224-UTR-E-siliculosus-1a_F_contig1305:11095..111551696318697.0100224-UTR-E-siliculosus-1a_F_contig1305:11095..11155Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig1305 11096..11155 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927827.1447113-CDS-E-siliculosus-1a_F_contig1305:11155..111681622927827.1447113-CDS-E-siliculosus-1a_F_contig1305:11155..11168Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1305 11156..11168 +
1696318697.019605-CDS-E-siliculosus-1a_F_contig1305:11155..111681696318697.019605-CDS-E-siliculosus-1a_F_contig1305:11155..11168Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1305 11156..11168 +
1622927827.162247-CDS-E-siliculosus-1a_F_contig1305:13582..138331622927827.162247-CDS-E-siliculosus-1a_F_contig1305:13582..13833Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1305 13583..13833 +
1696318697.0289185-CDS-E-siliculosus-1a_F_contig1305:13582..138331696318697.0289185-CDS-E-siliculosus-1a_F_contig1305:13582..13833Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1305 13583..13833 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig1305.1860.1

>prot_E-siliculosus-1a_F_contig1305.1860.1 ID=prot_E-siliculosus-1a_F_contig1305.1860.1|Name=mRNA_E-siliculosus-1a_F_contig1305.1860.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=88bp
MTTEGRSGCLDAFVVALNQACNGRWYASTLELQLVSLALCGACRSVPTLH
MRVNRQTPPRLWPVCTPHTTPNKSTPEVRISSCVPAIE
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mRNA from alignment at E-siliculosus-1a_F_contig1305:11096..13833+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig1305.1860.1 ID=mRNA_E-siliculosus-1a_F_contig1305.1860.1|Name=mRNA_E-siliculosus-1a_F_contig1305.1860.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=2738bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1305:11096..13833+ (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
CGGACCGAGTGAGTCATAACACCGGCACGCATCGTGTGGTTGGGTTGCAC
AGCGGTGGCCATGACTACCGAAGGTGAGAGAGGGTGTAGGGTTGGGTTGA
GGCTGCAACGGGAGACGGGGGACCAAAGAAATTCCAGGGGAAATACAACG
TCGGTGGCGGGTGTGCTTGCCACCTTCTTCGTGATATCCGCCCATCAGTC
CACAGCAAGGCTTCCCGTATGCTTCATATGATGGAGTGAGTTCCAGCCCA
CATCAACGAGAGAGCCAAAACACGATTGCGAAAACCTCTGCGAGTGACCC
AAGGCTACAAGTATCAATTACACGAACATCTTCCTGTGACTCCCAGTACT
GTACAGTACTGTAGCGCTCATTTGGCCGAAAGTGGTGGTCTCCATGTCGG
AATACAACTTTTTCTCGGAACATTCCTCGGTGTAGCTGCGGCTCGTGCAC
AACAATCTAGCTCTTCATCGCATTGCAACCTAGCGCTAACCGTGCAAAGC
AACCCAGCCAGCCCCTCTTGTGCCGCAGCTTGGGCTTACACGTAGAGCAA
CCTAGTGTTTCACCTGCCCCCTTAGGCCTGCCTGTACAGCATACGCCGTA
TGCATACCAACCTACCAGCTTGCAACCGTGCCAAATTGTTGCGCCACGGC
TTGCCGTGGCTTTGGCTTGCTATGTACAGTGACCCGTTTTGCGCTTGCCA
ATGTATGCGCCATTGAGAACACCATGAAGAGGCGTCTTGTATGCTGGCAC
GTTCAGGTTTGCTTCTTGGGTGGCGATATTGAGGATCTTCGTACAATAGT
ACTGGCCTCTGTACCATCTCCGCAAATCTCCGCGAATTGGAACAACATCC
AGTGTCTTGTACTGCTTGCGCTCACCGCCACGAACCGTAAACCCGTGAAC
GGAGGGTTTTCAGCAGTAGCTAGTGGCGTCAGCTACTGCTCAAAACCCGC
AGTTCAGGAGATTGGGATCAGTGGCGGCGAGCACAAGCAGTACAGGACAC
TGGGTGTCGGTCAAATTTACGGAGATTGTAGGCCATTACTACGATCTTCG
ATACCGCCACCACAGAGGCAGACTTGAACGTGTCAACACACCCTCCCCGC
TGCCACAGGGTGTCATCTAGTGATGCCAGTACTCAGGTGCAATTTCAATG
CTACACTGGCCTTATTTGAGAGCCTGCCGTTTCGACTCTTTCGACTTGAA
TTTACTGTAGACTTGTATGAATTTTTCAAGGGTGTGTTCGGTAAGGTACT
ACTACCGAAGCATACCGGCGGTAAATACCGCCGGTATATACCATACCGAG
CGTCTCGGTATACTTCGGTACTTTTTCAATACCTTACCGAAATACTCGGT
ATACTTCGGTACTTTTTCAATACCTTACCGAAATTCTCGGTATACTTCGG
TACTTTTTCAATACCTTACCGAAACTCTCGGTGTACTTAGGTACTTTATC
AATACCTTACCGACATTCTCAAGGGTGGTTCGGTAAGGTAATACCGAAGC
ATACCGGCGGTAATACCGGCGATATTTACCATACCGAGCGTCTCGGTATA
CTTCGGTACTTGTTCAATACCTTACCGAAATTCTCGGTATACTTCGGTAC
TTTTTCAATAACTTACCGAAAATCTCGGTATACTTCGGTACTTTTCGATA
CCTTACCGAAAATCTCGGTATGCCATTGGCAAATACCGAGGGTACCGTAA
ATACCTTGGAAATTTTTCAATACCTTACCGAAATTCTCGGTATACTTCGG
TACTTTTTTAATACCTTACCGAAGTTCTCGGTACCGAAATTCTCGGTATA
CTTCGGTACTTTTTCAATACCTTGCCGAAAATGTCGGTACGCTTTGGAAC
TTTTTCAATACCTTACCGAAAATACCCGTACCTTACCGAACACACCCTTG
GAATTTTTTATCTTGATTTCAACGGGGCGTGTCGAGGTAGACTCATGATA
TGTATGTTTTGAGCAGCCTCTGTAGCCGCCCAGTGCGAGAATGAACGATT
TCTGTTTGCCTCGATGCCTTCCGACTGCTCTTGTGTGTCTCGTGTGCATA
TTCTGTGCTCTTGCGGTTTACTTCAAAAACAGTCGGCCGCAAAATTGGTT
GGGTTCCCCCCCTTAGCATTGTCTATACTCGCACAAGGCTCACTTCACTT
CATCAACGCTGTACCCGGCGTATCAGGTGATTTCTCTTGCAACAGCCCAC
CGAATGGTACGTTTGCCGGGCTCGACTGTAATAGTGCTCGAAGGAGGGTG
TCGAACACGAACAGACACGAACAGGTGACTAGCCACAGGATATCCCTGTG
TTTGATTATCAACGGTTGTTATTGCCCCTTTTCTGGTGGCCGTGATCTTC
TTCGCATCGACTGTGAGAATCTCCCCCCCTCTCGTTCTGACTATCACCCA
CCGCGCAATCAACACTTGTTCCAAGGCCCACAAGCCATTATTCGTTTGCG
GTTGCTACGTGTGTGTATCTGCCCTCTACTGTCTCAGGAAGGAGTGGATG
CTTGGACGCCTTCGTCGTTGCCTTGAACCAGGCTTGCAATGGGCGTTGGT
ATGCATCTACGCTGGAGCTACAGCTTGTGTCCCTGGCGCTTTGCGGGGCG
TGCAGAAGTGTCCCGACCTTGCACATGCGAGTCAATCGGCAAACGCCCCC
TCGTTTGTGGCCTGTTTGCACGCCACACACCACTCCCAACAAGAGCACTC
CAGAGGTTCGCATCTCGAGTTGTGTGCCAGCTATTGAG
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig1305:11096..13833+

>mRNA_E-siliculosus-1a_F_contig1305.1860.1 ID=mRNA_E-siliculosus-1a_F_contig1305.1860.1|Name=mRNA_E-siliculosus-1a_F_contig1305.1860.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=528bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1305:11096..13833+ (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATGACTACCGAAGATGACTACCGAAGGAAGGAGTGGATGCTTGGACGCCT
TCGTCGTTGCCTTGAACCAGGCTTGCAATGGGCGTTGGTATGCATCTACG
CTGGAGCTACAGCTTGTGTCCCTGGCGCTTTGCGGGGCGTGCAGAAGTGT
CCCGACCTTGCACATGCGAGTCAATCGGCAAACGCCCCCTCGTTTGTGGC
CTGTTTGCACGCCACACACCACTCCCAACAAGAGCACTCCAGAGGTTCGC
ATCTCGAGTTGTGTGCCAGCTATTGAGGAAGGAGTGGATGCTTGGACGCC
TTCGTCGTTGCCTTGAACCAGGCTTGCAATGGGCGTTGGTATGCATCTAC
GCTGGAGCTACAGCTTGTGTCCCTGGCGCTTTGCGGGGCGTGCAGAAGTG
TCCCGACCTTGCACATGCGAGTCAATCGGCAAACGCCCCCTCGTTTGTGG
CCTGTTTGCACGCCACACACCACTCCCAACAAGAGCACTCCAGAGGTTCG
CATCTCGAGTTGTGTGCCAGCTATTGAG
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