mRNA_E-siliculosus-1a_F_contig1268.1643.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig1268.1643.1
Unique NamemRNA_E-siliculosus-1a_F_contig1268.1643.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig1268.1643.1 vs. uniprot
Match: D8LLF1_ECTSI (Kinesin (KAR3 subfamily) (Partial) (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LLF1_ECTSI)

HSP 1 Score: 479 bits (1232), Expect = 1.580e-154
Identity = 346/414 (83.57%), Postives = 350/414 (84.54%), Query Frame = 1 
Query:  241 GAGAIHEPQSFDHLRVPAKPSASSTRVVDAXXXXXXXXXXXXXXXXXXXXASARKRVGAAEQTRGIQMGPLRVRTPAKPKRSRADATPFHAPSTSSVPAPSAGVTHNYLRRGXXXXXXXXXXXXXSPTNRSSPTSXXXXXXRRPRSPSLAXXXXXXXXXXXXPPLHPSRIPCAAEPTAARPGAATTPGAAGSTRNRSSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAAPPVDGPSPMSLLSNTLSAIGLAVGVNSGGKPPRSPRASKGSPRLSISGSGSSPRVSPRXXXXXAARSRTSPFFDTSGSGGGCEGLPCMLEEDEEEDFPLGVARQSLESRLADAAAASSSLAAVAHRASVENMQN-SPATSLSPFTKSSTTVRRTSTSRYNVNGE 1479
            GAGAIHEPQSFDHLRVPAKPSASSTR+VDA         XXXXXXXXX  ASARKRVGAAEQTRGIQMGPLRVRTPAKPKRSRADATPFHAPS+SSVPAPSAG THNYLRRGXXXXXXXXXXXXX PTNRSSPTSXXXXXXRRPRSPSLA            PPLH SRIP AAEPTAARPGAA TPGAAGSTRNRSS                  XXXXXXXXXXXXXXXXXXXXXXXX        AA PVDGPSPMSLLSNTLSAIGLAVGVNS GKPPRSPRASKGSPRLS+SGSGSSPRVS  XXXXX   SRTSPFFDTSGSGGGCEGLP MLEEDEEEDFPLGV RQSLESRLADAAAASSSLAAVAHRAS+ENMQN SPATSLSPFTKSSTTVRRTSTSRYNVNGE
Sbjct:   82 GAGAIHEPQSFDHLRVPAKPSASSTRIVDAAANQHHLGAXXXXXXXXXL-ASARKRVGAAEQTRGIQMGPLRVRTPAKPKRSRADATPFHAPSSSSVPAPSAGATHNYLRRGXXXXXXXXXXXXXXPTNRSSPTSXXXXXXRRPRSPSLARSPTAAGAGRSSPPLHLSRIPRAAEPTAARPGAAATPGAAGSTRNRSSPRRQRHMSSSQALRNVAGXXXXXXXXXXXXXXXXXXXXXXXXAEGDGNGGAAAPVDGPSPMSLLSNTLSAIGLAVGVNSCGKPPRSPRASKGSPRLSVSGSGSSPRVSXXXXXXXXXXSRTSPFFDTSGSGGGCEGLPRMLEEDEEEDFPLGVTRQSLESRLADAAAASSSLAAVAHRASLENMQNNSPATSLSPFTKSSTTVRRTSTSRYNVNGE 494
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BLAST of mRNA_E-siliculosus-1a_F_contig1268.1643.1 vs. uniprot
Match: A0A6H5KIW8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KIW8_9PHAE)

HSP 1 Score: 460 bits (1184), Expect = 2.860e-154
Identity = 343/415 (82.65%), Postives = 346/415 (83.37%), Query Frame = 1 
Query:  241 GAGAIHEPQSFDHLRVPAKPSASSTRVVDAXXXXXXXXXXXXXXXXXXXXASARKRVGAAEQTRGIQMGPLRVRTPAKPKRSRADATPFHAPSTSSVPAPSAGVTHNYLRRGXXXXXXXXXXXXXS-PTNRSSPTSXXXXXXRRPRSPSLAXXXXXXXXXXXXPPLHPSRIPCAAEPTAARPGAATTPGAAGSTRNRSSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAAPPVDGPSPMSLLSNTLSAIGLAVGVNSGGKPPRSPRASKGSPRLSISGSGSSPRVSPRXXXXXAARSRTSPFFDTSGSGGGCEGLPCMLEEDEEEDFPLGVARQSLESRLADAAAASSSLAAVAHRASVENMQN-SPATSLSPFTKSSTTVRRTSTSRYNVNGE 1479
            GAGAIHEPQSFDHLRVPAKPS SSTRVVDA XXXXXXXXXXXXXXXXXXXASARKRVGAAEQTRGIQMGPLRVRTPAKPKRSRADATPFHAPS+SSVPAPSAG THNYLRRGXXXXXXXXXXXX S PTNRSSP  XXXXXX RPR     XXXXXXXXXXXX   HPSRIP AAEPTAARPGAA TPGAAGSTRNRSS                       XXXX                XXXXXXXAA PVDGPSPMSLLSNTLSAIGLAVGV   GKPPRSPRASKGSPRLS+SGSGSSPRVSP      AARSRTSPFFDTSGSGGGCEGLP MLEEDEEEDFPLGVARQSLESRLADAAAASS+LAAVAHRASVENMQN SPATSLSPFTKSSTTVRRTSTSRYNVNGE
Sbjct:   88 GAGAIHEPQSFDHLRVPAKPSTSSTRVVDAAXXXXXXXXXXXXXXXXXXXASARKRVGAAEQTRGIQMGPLRVRTPAKPKRSRADATPFHAPSSSSVPAPSAGATHNYLRRGXXXXXXXXXXXXASSPTNRSSPXXXXXXXXXRPRXXXXXXXXXXXXXXXXXXXXHPSRIPRAAEPTAARPGAAATPGAAGSTRNRSSPRRQRHMSSSQALRNVAGGNGEDXXXXEATFMENDNGDHDGGAXXXXXXXAAAPVDGPSPMSLLSNTLSAIGLAVGV---GKPPRSPRASKGSPRLSVSGSGSSPRVSPWSSPRAAARSRTSPFFDTSGSGGGCEGLPRMLEEDEEEDFPLGVARQSLESRLADAAAASSNLAAVAHRASVENMQNNSPATSLSPFTKSSTTVRRTSTSRYNVNGE 499
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig1268.1643.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
D8LLF1_ECTSI1.580e-15483.57Kinesin (KAR3 subfamily) (Partial) (Fragment) n=1 ... [more]
A0A6H5KIW8_9PHAE2.860e-15482.65Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig1268contigE-siliculosus-1a_F_contig1268:638..2673 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start1
Stop0
Cds size1479
Model size1479
Exons2
Ec32 orthologEc-27_002410.1
Ec32 ortholog descriptionkinesin (KAR3 subfamily)
Hectar predicted targeting categoryother localisation
BRITEko00000,ko03036,ko04812
Best eggNOG OGNA|NA|NA
Best tax levelEukaryota
COG Functional cat.Z
Ec32 orthologEc-27_002410.2
EggNOG OGsCOG5059@1,KOG0239@2759
EggNOG free text desc.microtubule motor activity
KEGG koko:K10405
Seed eggNOG ortholog2880.D8LLF1
Seed ortholog evalue5.6e-134
Seed ortholog score484.6
Taxonomic scopeEukaryota
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927791.2908707-CDS-E-siliculosus-1a_F_contig1268:637..8381622927791.2908707-CDS-E-siliculosus-1a_F_contig1268:637..838Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1268 638..838 -
1696318680.5691187-CDS-E-siliculosus-1a_F_contig1268:637..8381696318680.5691187-CDS-E-siliculosus-1a_F_contig1268:637..838Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1268 638..838 -
1622927791.3318357-CDS-E-siliculosus-1a_F_contig1268:1395..26731622927791.3318357-CDS-E-siliculosus-1a_F_contig1268:1395..2673Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1268 1396..2673 -
1696318680.5805266-CDS-E-siliculosus-1a_F_contig1268:1395..26731696318680.5805266-CDS-E-siliculosus-1a_F_contig1268:1395..2673Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1268 1396..2673 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig1268.1643.1prot_E-siliculosus-1a_F_contig1268.1643.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig1268 638..2673 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig1268.1643.1

>prot_E-siliculosus-1a_F_contig1268.1643.1 ID=prot_E-siliculosus-1a_F_contig1268.1643.1|Name=mRNA_E-siliculosus-1a_F_contig1268.1643.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=493bp
MMSSAAFERSLRGGASSSSSSMATSSSSSTSSLRPPKKKSVTWDDDEVLP
DDSGGESGVLRNSSIACTNTNSGGAAATAAGAGAIHEPQSFDHLRVPAKP
SASSTRVVDAAANQHHHGAGSAGGGGGGLLASARKRVGAAEQTRGIQMGP
LRVRTPAKPKRSRADATPFHAPSTSSVPAPSAGVTHNYLRRGGGTAAATA
AAVASSPTNRSSPTSAAGAAARRPRSPSLARSPTAAAAGRSSPPLHPSRI
PCAAEPTAARPGAATTPGAAGSTRNRSSPRRQRQMSSSQALRNVAGGNGE
DDDDAEATFMEDDSGDHGGGAEGDGNGGAAPPVDGPSPMSLLSNTLSAIG
LAVGVNSGGKPPRSPRASKGSPRLSISGSGSSPRVSPRSSPRAAARSRTS
PFFDTSGSGGGCEGLPCMLEEDEEEDFPLGVARQSLESRLADAAAASSSL
AAVAHRASVENMQNSPATSLSPFTKSSTTVRRTSTSRYNVNGE
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mRNA from alignment at E-siliculosus-1a_F_contig1268:638..2673-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig1268.1643.1 ID=mRNA_E-siliculosus-1a_F_contig1268.1643.1|Name=mRNA_E-siliculosus-1a_F_contig1268.1643.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=2036bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1268:638..2673- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATGATGTCTTCGGCGGCGTTCGAGCGCTCCTTGAGAGGGGGGGCATCATC
TTCGTCCTCGTCGATGGCGACCTCCTCCTCCTCCTCGACATCCTCGCTGA
GGCCCCCCAAGAAGAAGAGCGTCACGTGGGACGACGACGAGGTGCTTCCG
GACGACAGCGGCGGCGAATCCGGCGTGCTCCGGAACAGTAGCATCGCCTG
CACCAACACCAACAGCGGGGGTGCAGCAGCAACAGCAGCCGGGGCAGGTG
CAATCCACGAGCCGCAGAGCTTCGACCACCTCCGAGTGCCGGCCAAGCCT
TCGGCTAGCAGCACCCGAGTCGTCGACGCGGCCGCGAACCAACACCACCA
CGGCGCGGGCTCGGCCGGTGGCGGCGGCGGCGGCCTGTTGGCCTCCGCTC
GCAAGAGGGTGGGAGCGGCGGAGCAGACGCGAGGCATCCAGATGGGGCCC
CTGCGAGTGCGGACCCCGGCCAAGCCCAAGCGGTCTCGCGCGGACGCGAC
GCCCTTCCACGCCCCGTCCACTTCTTCCGTTCCCGCTCCCAGCGCGGGCG
TCACACACAACTACCTCCGGCGCGGCGGCGGGACGGCGGCAGCGACAGCG
GCAGCGGTGGCGTCGTCGCCGACTAATAGATCGTCTCCTACTTCCGCCGC
CGGTGCCGCTGCCCGCCGCCCGAGGAGTCCCAGCCTGGCAAGGTCGCCTA
CTGCCGCCGCTGCTGGCAGATCGTCTCCCCCGCTCCATCCAAGTAGAATC
CCTTGCGCCGCCGAGCCGACTGCTGCCAGGCCCGGCGCGGCGACTACACC
TGGGGCCGCGGGCAGCACCAGGAATCGCAGCAGCCCCCGTCGGCAGAGGC
AAATGTCTTCGAGCCAAGCGCTGCGCAACGTCGCTGGGGGCAACGGCGAA
GACGACGACGACGCCGAAGCTACCTTCATGGAGGACGACAGCGGAGATCA
CGGCGGGGGTGCGGAGGGAGACGGCAACGGCGGTGCGGCGCCCCCTGTGG
ACGGCCCATCCCCGATGTCTCTCCTGTCGAACACTCTCTCGGCAATCGGC
CTCGCGGTCGGGGTAAATTCCGGCGGAAAGCCACCCAGGTCGCCCAGGGC
TAGCAAGGGCTCCCCCAGGCTCAGCATTAGCGGTAGCGGCAGTTCCCCTA
GGGTTTCTCCGAGGAGCTCTCCGAGAGCTGCTGCGAGAAGCCGCACGTCG
CCGTTTTTTGACACCTCCGGGAGCGGCGGCGGCTGCGAGGGCCTCCCGTG
TATGCTGGAAGAAGACGAGGAAGAAGACGTGAGTTTAATGTGTCTCGCCC
AGTGGGCGGCACAGCAAGCGCTTATACGTGTATAGTAGCACAGGCAGTCG
GCCCTCTCTTGTTGGTTTTGAACGCGTCTCGTGACGTGCAGGCCTGTTTT
CGCTGCGCTGCAGCGCGTGGAGCGCCTGGTTGTGCACATGTCTGGTCTGT
ACCCGCTCTCGCCCTCTCTGTAGACTGCAATGAGAGCAGGGGAAGTCGGG
AATTAACATCAATAGCACCTACTGGTTGGGATCACACTTCAGTGTTTCAC
ATCTTAGCGAGGGATGCTGTGCATAACTTCCACGCATTCGAACGGCTGTC
CTGATGTGTAGGATAACGAGGTCTTCCGAACTCGCTCTGTCGCTGTACCG
TATGTTCGAGATGTTATCACCCTCTGCTACAGCGCTGTTGGTTCCCCGCC
CCTCTCCTCCTCGCCCGCACATTACCTTACCCCGTTCCCCCGAACAGAAT
CACGTGTAGCGTGACTTCGAATGATTGCACCGTGTCCCATATATATATAT
ATATATACTTTTTTTTTTCCCGGTCTGCACGGCAGTTCCCCCTGGGCGTG
GCTCGGCAATCTCTGGAGAGCCGATTGGCGGACGCGGCGGCCGCGAGCTC
GAGCCTGGCGGCCGTCGCTCACAGGGCCTCCGTGGAAAACATGCAGAACA
GTCCGGCTACCTCGCTGAGCCCTTTCACCAAGAGCAGCACCACAGTCAGG
CGGACCTCGACCAGTCGCTACAACGTCAACGGCGAG
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig1268:638..2673-

>mRNA_E-siliculosus-1a_F_contig1268.1643.1 ID=mRNA_E-siliculosus-1a_F_contig1268.1643.1|Name=mRNA_E-siliculosus-1a_F_contig1268.1643.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=2958bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1268:638..2673- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATGATGTCTTCGGCGGCGTTCGAGCGCTCCTTGAGAGGGGGGGCATCATC
TTCGTCCTCGTCGATGGCGACCTCCTCCTCCTCCTCGACATCCTCGCTGA
GGCCCCCCAAGAAGAAGAGCGTCACGTGGGACGACGACGAGGTGCTTCCG
GACGACAGCGGCGGCGAATCCGGCGTGCTCCGGAACAGTAGCATCGCCTG
CACCAACACCAACAGCGGGGGTGCAGCAGCAACAGCAGCCGGGGCAGGTG
CAATCCACGAGCCGCAGAGCTTCGACCACCTCCGAGTGCCGGCCAAGCCT
TCGGCTAGCAGCACCCGAGTCGTCGACGCGGCCGCGAACCAACACCACCA
CGGCGCGGGCTCGGCCGGTGGCGGCGGCGGCGGCCTGTTGGCCTCCGCTC
GCAAGAGGGTGGGAGCGGCGGAGCAGACGCGAGGCATCCAGATGGGGCCC
CTGCGAGTGCGGACCCCGGCCAAGCCCAAGCGGTCTCGCGCGGACGCGAC
GCCCTTCCACGCCCCGTCCACTTCTTCCGTTCCCGCTCCCAGCGCGGGCG
TCACACACAACTACCTCCGGCGCGGCGGCGGGACGGCGGCAGCGACAGCG
GCAGCGGTGGCGTCGTCGCCGACTAATAGATCGTCTCCTACTTCCGCCGC
CGGTGCCGCTGCCCGCCGCCCGAGGAGTCCCAGCCTGGCAAGGTCGCCTA
CTGCCGCCGCTGCTGGCAGATCGTCTCCCCCGCTCCATCCAAGTAGAATC
CCTTGCGCCGCCGAGCCGACTGCTGCCAGGCCCGGCGCGGCGACTACACC
TGGGGCCGCGGGCAGCACCAGGAATCGCAGCAGCCCCCGTCGGCAGAGGC
AAATGTCTTCGAGCCAAGCGCTGCGCAACGTCGCTGGGGGCAACGGCGAA
GACGACGACGACGCCGAAGCTACCTTCATGGAGGACGACAGCGGAGATCA
CGGCGGGGGTGCGGAGGGAGACGGCAACGGCGGTGCGGCGCCCCCTGTGG
ACGGCCCATCCCCGATGTCTCTCCTGTCGAACACTCTCTCGGCAATCGGC
CTCGCGGTCGGGGTAAATTCCGGCGGAAAGCCACCCAGGTCGCCCAGGGC
TAGCAAGGGCTCCCCCAGGCTCAGCATTAGCGGTAGCGGCAGTTCCCCTA
GGGTTTCTCCGAGGAGCTCTCCGAGAGCTGCTGCGAGAAGCCGCACGTCG
CCGTTTTTTGACACCTCCGGGAGCGGCGGCGGCTGCGAGGGCCTCCCGTG
TATGCTGGAAGAAGACGAGGAAGAAGACATGATGTCTTCGGCGGCGTTCG
AGCGCTCCTTGAGAGGGGGGGCATCATCTTCGTCCTCGTCGATGGCGACC
TCCTCCTCCTCCTCGACATCCTCGCTGAGGCCCCCCAAGAAGAAGAGCGT
CACGTGGGACGACGACGAGGTGCTTCCGGACGACAGCGGCGGCGAATCCG
GCGTGCTCCGGAACAGTAGCATCGCCTGCACCAACACCAACAGCGGGGGT
GCAGCAGCAACAGCAGCCGGGGCAGGTGCAATCCACGAGCCGCAGAGCTT
CGACCACCTCCGAGTGCCGGCCAAGCCTTCGGCTAGCAGCACCCGAGTCG
TCGACGCGGCCGCGAACCAACACCACCACGGCGCGGGCTCGGCCGGTGGC
GGCGGCGGCGGCCTGTTGGCCTCCGCTCGCAAGAGGGTGGGAGCGGCGGA
GCAGACGCGAGGCATCCAGATGGGGCCCCTGCGAGTGCGGACCCCGGCCA
AGCCCAAGCGGTCTCGCGCGGACGCGACGCCCTTCCACGCCCCGTCCACT
TCTTCCGTTCCCGCTCCCAGCGCGGGCGTCACACACAACTACCTCCGGCG
CGGCGGCGGGACGGCGGCAGCGACAGCGGCAGCGGTGGCGTCGTCGCCGA
CTAATAGATCGTCTCCTACTTCCGCCGCCGGTGCCGCTGCCCGCCGCCCG
AGGAGTCCCAGCCTGGCAAGGTCGCCTACTGCCGCCGCTGCTGGCAGATC
GTCTCCCCCGCTCCATCCAAGTAGAATCCCTTGCGCCGCCGAGCCGACTG
CTGCCAGGCCCGGCGCGGCGACTACACCTGGGGCCGCGGGCAGCACCAGG
AATCGCAGCAGCCCCCGTCGGCAGAGGCAAATGTCTTCGAGCCAAGCGCT
GCGCAACGTCGCTGGGGGCAACGGCGAAGACGACGACGACGCCGAAGCTA
CCTTCATGGAGGACGACAGCGGAGATCACGGCGGGGGTGCGGAGGGAGAC
GGCAACGGCGGTGCGGCGCCCCCTGTGGACGGCCCATCCCCGATGTCTCT
CCTGTCGAACACTCTCTCGGCAATCGGCCTCGCGGTCGGGGTAAATTCCG
GCGGAAAGCCACCCAGGTCGCCCAGGGCTAGCAAGGGCTCCCCCAGGCTC
AGCATTAGCGGTAGCGGCAGTTCCCCTAGGGTTTCTCCGAGGAGCTCTCC
GAGAGCTGCTGCGAGAAGCCGCACGTCGCCGTTTTTTGACACCTCCGGGA
GCGGCGGCGGCTGCGAGGGCCTCCCGTGTATGCTGGAAGAAGACGAGGAA
GAAGACTTCCCCCTGGGCGTGGCTCGGCAATCTCTGGAGAGCCGATTGGC
GGACGCGGCGGCCGCGAGCTCGAGCCTGGCGGCCGTCGCTCACAGGGCCT
CCGTGGAAAACATGCAGAACAGTCCGGCTACCTCGCTGAGCCCTTTCACC
AAGAGCAGCACCACAGTCAGGCGGACCTCGACCAGTCGCTACAACGTCAA
CGGCGAGTTCCCCCTGGGCGTGGCTCGGCAATCTCTGGAGAGCCGATTGG
CGGACGCGGCGGCCGCGAGCTCGAGCCTGGCGGCCGTCGCTCACAGGGCC
TCCGTGGAAAACATGCAGAACAGTCCGGCTACCTCGCTGAGCCCTTTCAC
CAAGAGCAGCACCACAGTCAGGCGGACCTCGACCAGTCGCTACAACGTCA
ACGGCGAG
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