mRNA_E-siliculosus-1a_F_contig1259.1594.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig1259.1594.1
Unique NamemRNA_E-siliculosus-1a_F_contig1259.1594.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig1259.1594.1 vs. uniprot
Match: D7FVI9_ECTSI (Ankyrin repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FVI9_ECTSI)

HSP 1 Score: 84.3 bits (207), Expect = 5.430e-14
Identity = 80/231 (34.63%), Postives = 107/231 (46.32%), Query Frame = 1 
Query:  544 EDSRLVLIPLVDTASQGPRFVIEVLLDASIDINTHCTHNSSTTLHYAAREGHHRIVAALLHMGVDKDTLDAAGQTPLIVAASRGLLPVVKTLLNADAGVHLPR--TNDKNY---------HSSPLRCHWRNDGTVAALLD-GGADMNASALPAKPHPFWQPAMATA-PS*ETLLEAGAGVNIRFAWRSTS-LRRSTALHFAARSGHNWAVNALLRGGAETDATGTYGHTPL 1194
            E  +    PL+  A  G   V+E LLD   D       + ST++  AA  G++ IV ALL+ G DKD+  A G+TPL+ A+ +G LPVVKTLLNADA V L      D++Y         H +    H    G V ALL  GG +++A              M  + P  ETLL AGA  +IR            TA H     G       L+  GA+ ++   +G TPL
Sbjct:   81 EQDQFGYTPLMKAAENGHMPVMETLLDQGADATFRHLDDGSTSIIAAALGGYNEIVIALLNKGADKDSPAANGRTPLMEASRKGHLPVVKTLLNADADVTLAAHGLTDEDYCLGDNCTALHFATAGGHL---GVVNALLSKGGLEIDAVDTDGTTALHLAVIMGRSLPIMETLLVAGADPDIRINTGGVQDCPLETACHSKDNIGTYHVCKTLIDHGADVNSRCPFGSTPL 308
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BLAST of mRNA_E-siliculosus-1a_F_contig1259.1594.1 vs. uniprot
Match: A0A2K3CTM6_CHLRE (HET domain-containing protein n=1 Tax=Chlamydomonas reinhardtii TaxID=3055 RepID=A0A2K3CTM6_CHLRE)

HSP 1 Score: 60.8 bits (146), Expect = 4.240e-6
Identity = 47/132 (35.61%), Postives = 64/132 (48.48%), Query Frame = 1 
Query:  568 PLVDTASQGPRFVIEVLLDASIDINTHCTHNS-STTLHYAAREGHHRIVAALLHMGVDKDTLDAAGQTPLIVAASRGLLPVVKTLLNADAGVHLPRTNDKNYHSSPLRCHWRNDGTVAALLDGGADMNASAL 960
            PL+     G   V+ VLL+A   +    +    +T LH AA  G   +V ALL  G +    DAAG TPL VAA  G LPVV+ LL    G  +   +  N  ++PL     ++   AAL   GA++ A  L
Sbjct:  607 PLLMAVCAGHVEVVHVLLEAGAAVQVCLSEEERNTALHVAAARGDPAVVQALLAAGANIGARDAAGCTPLSVAAREGHLPVVELLLATSGGKDVDAKD--NAGNTPLHHSAIHEDVAAALQAAGANVAARNL 736
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BLAST of mRNA_E-siliculosus-1a_F_contig1259.1594.1 vs. uniprot
Match: A0A4Y8CVW4_9HELO (NACHT domain-containing protein n=15 Tax=Sclerotiniaceae TaxID=28983 RepID=A0A4Y8CVW4_9HELO)

HSP 1 Score: 59.7 bits (143), Expect = 8.820e-6
Identity = 60/206 (29.13%), Postives = 85/206 (41.26%), Query Frame = 1 
Query:  682 AAREGHHRIVAALLHMGVDKDTLDAAGQTPLIVAASRGLLPVVKTLLNADAG--------------------------VHLPRTNDKNYHSSPLRCHWRNDGTVAALLDGGADMNASALPAKPHPFWQPAMATAPS*ETLLEAGAGVNIRFAWRSTSLRRSTALHFAARSGHNWAVNALLRGGAETDA-TGTYGHTPLVAAGHEGH 1218
            AA  G   IV  LL  G D +T+     TPL++AA  G  PVV+ L+N+ A                           + +P   + N  +  +    RN+G + AL++ GAD+NAS   +   P  +  +   P    L            +R  +     AL  AA +G+  A+  LL  GAE DA   TY  T LV A   GH
Sbjct:  680 AAMFGLPTIVGKLLKTGADIETVCPKDFTPLLIAAELGHSPVVQILINSKANLLAKDYQQENALHKAARKGHVEIPSLIEIPFGENFNKTALHIAIDRRNEGVIRALIEAGADVNASDELSL-TPLMKSVVDNLPDTIDLXXXXXXXXXXXGFREHA-----ALSMAATAGNIVAIKKLLAAGAEVDAGKDTYSGTALVRAASSGH 879
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BLAST of mRNA_E-siliculosus-1a_F_contig1259.1594.1 vs. uniprot
Match: H2UME2_TAKRU (Uncharacterized protein n=1 Tax=Takifugu rubripes TaxID=31033 RepID=H2UME2_TAKRU)

HSP 1 Score: 59.3 bits (142), Expect = 1.190e-5
Identity = 52/168 (30.95%), Postives = 78/168 (46.43%), Query Frame = 1 
Query:  532 SHG----IEDSRLVLIPLVDTASQGPRFVIEVLLD-ASIDINTHCTHNSSTTLHYAAREGHHRIVAALLHMGVDKDTLD---AAGQTPLIVAASRGLLPVVKTLLNADAGVHLPRTNDKNYHSSPLRCHWRNDGTVAALLDGGADMNASALPAKPHPFWQPAMATAPS 1011
            SHG    + D+RL   PL+  A  G    +EVL++ A +D+     H + T LH A  +GH      +L    D++ ++   AA QTPL VAA RGL  VV+ LL  +A V     N +N +++ L                 +   AS  PA     + PA++  P+
Sbjct:  841 SHGAQANVADTRLCRTPLMMAALNGQTNTVEVLVNSAKVDLTLQDAHRN-TALHLACSKGHETCALLILEKIGDRNLINCTNAALQTPLHVAARRGLTVVVQELLGKEASVLAVDENGENENAAGLSXXXXXXXXXXXXXSPASCFTASLCPAG----YTPALSCTPN 1003
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BLAST of mRNA_E-siliculosus-1a_F_contig1259.1594.1 vs. uniprot
Match: A0A4Z2BL08_9TELE (Uncharacterized protein n=3 Tax=Takifugu TaxID=31032 RepID=A0A4Z2BL08_9TELE)

HSP 1 Score: 59.3 bits (142), Expect = 1.190e-5
Identity = 48/134 (35.82%), Postives = 68/134 (50.75%), Query Frame = 1 
Query:  532 SHG----IEDSRLVLIPLVDTASQGPRFVIEVLLD-ASIDINTHCTHNSSTTLHYAAREGHHRIVAALLHMGVDKDTLD---AAGQTPLIVAASRGLLPVVKTLLNADAGVHLPRTNDKNYHS---SPLRCHWR 900
            SHG    + D+RL   PL+  A  G    +EVL++ A +D+     H + T LH A  +GH      +L    D++ ++   AA QTPL VAA RGL  VV+ LL  +A V     N +N ++   SP  C  R
Sbjct:  884 SHGAQANVADTRLCRTPLMMAALNGQTNTVEVLVNSAKVDLTLQDAHRN-TALHLACSKGHETCALLILEKISDRNLINCTNAALQTPLHVAARRGLTVVVQELLGKEASVLAVDENGENENAAGLSPPSCFTR 1016
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BLAST of mRNA_E-siliculosus-1a_F_contig1259.1594.1 vs. uniprot
Match: D7FK36_ECTSI (Protein kinase domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FK36_ECTSI)

HSP 1 Score: 57.8 bits (138), Expect = 3.350e-5
Identity = 38/97 (39.18%), Postives = 55/97 (56.70%), Query Frame = 1 
Query:  673 LHYAAREGHHRIVAALLHMGVDKDTLDAAGQTPLIVAASRGLLPVVKTLLNADAGVHLPRT-NDKNYHSSPLRCHWRND--GTVAALLDGGADMNAS 954
            LH A R G    V+ LL  G D  +LD +G++PL++A   G LPVV+ LL+ DAG     T N  N  S+ ++   R +  G + AL+  GAD+ A+
Sbjct:   69 LHSAVRRGEADAVSKLLSEGADAASLDGSGRSPLLLATQEGHLPVVRVLLDGDAGDAACITPNGPNGMSALVKSAARKEHLGILRALIARGADVEAT 165
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BLAST of mRNA_E-siliculosus-1a_F_contig1259.1594.1 vs. uniprot
Match: Q8AX92_TAKRU (Uncharacterized protein n=1 Tax=Takifugu rubripes TaxID=31033 RepID=Q8AX92_TAKRU)

HSP 1 Score: 57.8 bits (138), Expect = 3.680e-5
Identity = 46/128 (35.94%), Postives = 66/128 (51.56%), Query Frame = 1 
Query:  532 SHG----IEDSRLVLIPLVDTASQGPRFVIEVLLD-ASIDINTHCTHNSSTTLHYAAREGHHRIVAALLHMGVDKDTLD---AAGQTPLIVAASRGLLPVVKTLLNADAGVHLPRTNDKNYHSSPLRC 891
            SHG    + D+RL   PL+  A  G    +EVL++ A +D+     H + T LH A  +GH      +L    D++ ++   AA QTPL VAA RGL  VV+ LL  +A V      D+N ++  L C
Sbjct:  965 SHGAQANVADTRLCRTPLMMAALNGQTNTVEVLVNSAKVDLTLQDAHRN-TALHLACSKGHETCALLILEKIGDRNLINCTNAALQTPLHVAARRGLTVVVQELLGKEASV---LAVDENGYTPALSC 1088
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig1259.1594.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 7
Match NameE-valueIdentityDescription
D7FVI9_ECTSI5.430e-1434.63Ankyrin repeat protein n=1 Tax=Ectocarpus siliculo... [more]
A0A2K3CTM6_CHLRE4.240e-635.61HET domain-containing protein n=1 Tax=Chlamydomona... [more]
A0A4Y8CVW4_9HELO8.820e-629.13NACHT domain-containing protein n=15 Tax=Sclerotin... [more]
H2UME2_TAKRU1.190e-530.95Uncharacterized protein n=1 Tax=Takifugu rubripes ... [more]
A0A4Z2BL08_9TELE1.190e-535.82Uncharacterized protein n=3 Tax=Takifugu TaxID=310... [more]
D7FK36_ECTSI3.350e-539.18Protein kinase domain-containing protein n=2 Tax=E... [more]
Q8AX92_TAKRU3.680e-535.94Uncharacterized protein n=1 Tax=Takifugu rubripes ... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig1259contigE-siliculosus-1a_F_contig1259:6288..8740 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start1
Stop1
Cds size504
Model size1352
Exons2
Hectar predicted targeting categoryother localisation
Best eggNOG OGNA|NA|NA
Best tax levelEukaryota
COG Functional cat.G
EggNOG OGsCOG0666@1,KOG4177@2759
EggNOG free text desc.response to abiotic stimulus
Seed eggNOG ortholog2880.D7FVI9
Seed ortholog evalue5.4e-12
Seed ortholog score77.8
Taxonomic scopeEukaryota
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig1259.1594.1prot_E-siliculosus-1a_F_contig1259.1594.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig1259 7899..8402 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927780.608747-UTR-E-siliculosus-1a_F_contig1259:6287..65361622927780.608747-UTR-E-siliculosus-1a_F_contig1259:6287..6536Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig1259 6288..6536 +
1696318676.9489272-UTR-E-siliculosus-1a_F_contig1259:6287..65361696318676.9489272-UTR-E-siliculosus-1a_F_contig1259:6287..6536Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig1259 6288..6536 +
1622927780.6209052-UTR-E-siliculosus-1a_F_contig1259:7637..78981622927780.6209052-UTR-E-siliculosus-1a_F_contig1259:7637..7898Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig1259 7638..7898 +
1696318676.960166-UTR-E-siliculosus-1a_F_contig1259:7637..78981696318676.960166-UTR-E-siliculosus-1a_F_contig1259:7637..7898Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig1259 7638..7898 +
1622927780.6476238-UTR-E-siliculosus-1a_F_contig1259:8402..87401622927780.6476238-UTR-E-siliculosus-1a_F_contig1259:8402..8740Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig1259 8403..8740 +
1696318676.9803836-UTR-E-siliculosus-1a_F_contig1259:8402..87401696318676.9803836-UTR-E-siliculosus-1a_F_contig1259:8402..8740Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig1259 8403..8740 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927780.6330256-CDS-E-siliculosus-1a_F_contig1259:7898..84021622927780.6330256-CDS-E-siliculosus-1a_F_contig1259:7898..8402Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1259 7899..8402 +
1696318676.969687-CDS-E-siliculosus-1a_F_contig1259:7898..84021696318676.969687-CDS-E-siliculosus-1a_F_contig1259:7898..8402Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1259 7899..8402 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig1259.1594.1

>prot_E-siliculosus-1a_F_contig1259.1594.1 ID=prot_E-siliculosus-1a_F_contig1259.1594.1|Name=mRNA_E-siliculosus-1a_F_contig1259.1594.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=168bp
MQRHVPESHGIEDSRLVLIPLVDTASQGPRFVIEVLLDASIDINTHCTHN
SSTTLHYAAREGHHRIVAALLHMGVDKDTLDAAGQTPLIVAASRGLLPVV
KTLLNADAGVHLPRTNDKNYHSSPLRCHWRNDGTVAALLDGGADMNASAL
PAKPHPFWQPAMATAPS*
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mRNA from alignment at E-siliculosus-1a_F_contig1259:6288..8740+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig1259.1594.1 ID=mRNA_E-siliculosus-1a_F_contig1259.1594.1|Name=mRNA_E-siliculosus-1a_F_contig1259.1594.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=2453bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1259:6288..8740+ (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
CTGACTGTTTCTTCCAAATAACAAAAAAAAAACGAAAAACTGTGGCTCAT
CGTGTCCGTCATCTTTTTTTATGACCGGGATCGTTTGAACGTTTTCATCG
GAAAATTATTTTCCGGGTGCGTCTTGTTTCGCGAAGCTTGTTTTCCCGAT
AACCCCTTCCTCCCTAGGCTCTCTTTCGGGATGAAGCGCGCTCCTGACCG
AGCCTTCATGTGAATCCGAGGGTGACTAGACTGTACCTTTGGCGATAAGG
TGAGTCTGTGGGGAAACTGCATTAACGAACTGCAACGAATCGCCTACTAC
GTTGGCTCCAATCAAAAGGCAAACGATATAATCAAAATGAAAGTCACGAG
CAATGTCTACCTGGGCGGCCAAATAATCAAATACTAGTGGGACCGATAAG
GGCGCGAGGTTGAAGCTTCCGGCGAGGACGAGGTGTTTGCCGAGTTGTAG
CGTTTGGGGAAAAGTAGCATGCCTCTGCAAAGGTATATTTGTCCGTCTCT
CTTCGTGTAAACGCGTGTGCGCGTGTCTGACGGACCAGCCAGCCGTTGAC
GAGGGGCGCATTTTGGACAACAGAAGCGACGATACGGGCTACCAGGTGCC
ACCGCAGCAGGCAGGAATGACAATTTTCCATTCCTATGGTATCAAGGTGT
CTTTCTGCGTGCGAAAGTTTGTTTTTAGCGCGCCTACTGACTTCTCTCGT
TTCGTGCGTGCGTGTGGAAATTACTTTCTGTGTCACCCCCCCCCCCCCCC
TCCAAAGGTTCTTTTTGACAAACACCCCCCATCGGCCTAAAAATGTGTAT
TGAGAGGTCGGGGGTACCTCTCTCTGAAAACTGCGTGTACATGTTTTGCT
CGATATCTCGGCCGCACATCATCCGTTGCCTAGATCTATCTACATTAACA
CACATACTCAGAGTTGATCTGAGAAGATCTCGATATCCCCATTTTTTAGG
CAAGCCTACATGAAAATTGATAGTTATCTAAGTCGTGGCCAATTCGTTTG
TTGATGTCAAAACAAATTGCACGCGAGTGTGCCTGGCCCACACGCGTCAA
TTCCAAAAATAACTTTAGAAATCGAAGTGAATATAGGAGGCTAGCTCCCA
GGTTCGCAAGGTATATATCTCTTTGGGGATGTTGTGCGGTGAGGAGTACC
TACCGGTTGCCTCACTGTCGCACGGAGAGAGAGAGGAACGACACAAGCTC
TCCCATTGTTTGTGACTACAACAAGTCGCATTTTTGTCTTGATGAGCTGA
CTCGGCAACAGGCGATTCTCCGGCTCGATTTTTCACCATTTTTCTATCTC
TGATCAGGTATCATCGGTGATGAACCCCCTACTCCTCCTATCACACACAG
GACATGCCAACAGACGCAAGGAGGGACGACCAACAGCTCGCGCTGGGGGC
GGGAGGCAACGACCGGGATCGTGACCGGGGTGACCGACGGCCCAACGGTG
TGATGGCGGCTGCATCGGCTATATATACCGGCTACCGCTTTCGGCACCAC
ATCTGCTGCTGCCGCTGCTGCTCCTGCGCTGGTTACACCATCCGATGTTA
GCACCCACCTCATGAGAATCAATGTCCCTCCGGAGTAGGTGTACGTGACG
GGATTCTTGCGATGCAGAGACATGTGCCCGAAAGCCACGGCATAGAAGAC
TCAAGATTGGTGTTGATCCCGTTGGTCGACACAGCCAGTCAAGGTCCACG
TTTTGTCATTGAGGTTCTGCTGGACGCCAGTATCGATATCAACACCCACT
GCACCCACAACAGCTCGACGACTCTTCACTATGCTGCCCGTGAAGGACAT
CATAGGATCGTGGCTGCTCTTCTACACATGGGTGTGGACAAGGATACACT
CGACGCTGCTGGTCAAACTCCATTGATTGTGGCAGCCTCTCGTGGCCTTC
TTCCTGTCGTGAAGACTCTGCTGAACGCCGACGCTGGGGTCCATCTCCCC
CGCACTAACGACAAGAACTATCACAGCTCTCCGCTTCGCTGCCATTGGAG
GAATGATGGAACGGTGGCTGCTCTTTTGGACGGAGGAGCGGACATGAATG
CCTCGGCACTACCGGCAAAACCCCATCCATTCTGGCAGCCGGCAATGGCC
ACAGCTCCGTCATAAGAGACTCTGCTCGAAGCCGGCGCCGGTGTCAATAT
CCGCTTCGCTTGGAGAAGCACCAGCCTCAGAAGGTCTACCGCCCTCCACT
TTGCCGCCCGAAGTGGACACAACTGGGCGGTGAATGCTCTTTTGAGAGGG
GGAGCAGAGACGGACGCAACCGGCACTTACGGTCATACCCCGTTGGTTGC
CGCAGGCCATGAGGGTCATTGTTCTGTCGTTGAGAGTTGCTGGCCGCCGG
TGCCGACCCCAACATCCGCAGCAGTAGTGGCGGGTATTTTACGCCCGGCT
CGAGTGTGCTGTCGGCGAGATGGACATCGGTATGATCAAAGCCCTTATTC
GTC
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig1259:6288..8740+

>mRNA_E-siliculosus-1a_F_contig1259.1594.1 ID=mRNA_E-siliculosus-1a_F_contig1259.1594.1|Name=mRNA_E-siliculosus-1a_F_contig1259.1594.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=1008bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1259:6288..8740+ (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATGCAGAGACATGTGCCCGAAAGCCACGGCATAGAAGACTCAAGATTGGT
GTTGATCCCGTTGGTCGACACAGCCAGTCAAGGTCCACGTTTTGTCATTG
AGGTTCTGCTGGACGCCAGTATCGATATCAACACCCACTGCACCCACAAC
AGCTCGACGACTCTTCACTATGCTGCCCGTGAAGGACATCATAGGATCGT
GGCTGCTCTTCTACACATGGGTGTGGACAAGGATACACTCGACGCTGCTG
GTCAAACTCCATTGATTGTGGCAGCCTCTCGTGGCCTTCTTCCTGTCGTG
AAGACTCTGCTGAACGCCGACGCTGGGGTCCATCTCCCCCGCACTAACGA
CAAGAACTATCACAGCTCTCCGCTTCGCTGCCATTGGAGGAATGATGGAA
CGGTGGCTGCTCTTTTGGACGGAGGAGCGGACATGAATGCCTCGGCACTA
CCGGCAAAACCCCATCCATTCTGGCAGCCGGCAATGGCCACAGCTCCGTC
ATAAATGCAGAGACATGTGCCCGAAAGCCACGGCATAGAAGACTCAAGAT
TGGTGTTGATCCCGTTGGTCGACACAGCCAGTCAAGGTCCACGTTTTGTC
ATTGAGGTTCTGCTGGACGCCAGTATCGATATCAACACCCACTGCACCCA
CAACAGCTCGACGACTCTTCACTATGCTGCCCGTGAAGGACATCATAGGA
TCGTGGCTGCTCTTCTACACATGGGTGTGGACAAGGATACACTCGACGCT
GCTGGTCAAACTCCATTGATTGTGGCAGCCTCTCGTGGCCTTCTTCCTGT
CGTGAAGACTCTGCTGAACGCCGACGCTGGGGTCCATCTCCCCCGCACTA
ACGACAAGAACTATCACAGCTCTCCGCTTCGCTGCCATTGGAGGAATGAT
GGAACGGTGGCTGCTCTTTTGGACGGAGGAGCGGACATGAATGCCTCGGC
ACTACCGGCAAAACCCCATCCATTCTGGCAGCCGGCAATGGCCACAGCTC
CGTCATAA
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