mRNA_E-siliculosus-1a_F_contig12350.1470.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig12350.1470.1
Unique NamemRNA_E-siliculosus-1a_F_contig12350.1470.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig12350.1470.1 vs. uniprot
Match: D8LI64_ECTSI (Catalytic, putative n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LI64_ECTSI)

HSP 1 Score: 125 bits (315), Expect = 1.410e-34
Identity = 63/64 (98.44%), Postives = 63/64 (98.44%), Query Frame = 1 
Query:   22 EHLDARLTDLGKQQCATLKATKHGIEKEAQLVVVSPLTRAIQTATLTIDQVEGVPWVALECVRE 213
            EHLDARLTDLGKQQCATLKATKHGIEKEAQLVVVSPL RAIQTATLTIDQVEGVPWVALECVRE
Sbjct:   56 EHLDARLTDLGKQQCATLKATKHGIEKEAQLVVVSPLARAIQTATLTIDQVEGVPWVALECVRE 119
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BLAST of mRNA_E-siliculosus-1a_F_contig12350.1470.1 vs. uniprot
Match: D8LI65_ECTSI (Catalytic, putative n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LI65_ECTSI)

HSP 1 Score: 116 bits (290), Expect = 8.210e-31
Identity = 57/64 (89.06%), Postives = 60/64 (93.75%), Query Frame = 1 
Query:   22 EHLDARLTDLGKQQCATLKATKHGIEKEAQLVVVSPLTRAIQTATLTIDQVEGVPWVALECVRE 213
            EHLDARLTDLGK+QCATLKA  HGIEKEA+LVVVSPLTRAI+TA L IDQVEGVPWVALECVRE
Sbjct:   56 EHLDARLTDLGKEQCATLKAANHGIEKEAELVVVSPLTRAIETAMLAIDQVEGVPWVALECVRE 119
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BLAST of mRNA_E-siliculosus-1a_F_contig12350.1470.1 vs. uniprot
Match: A0A835YRM9_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YRM9_9STRA)

HSP 1 Score: 70.5 bits (171), Expect = 4.540e-13
Identity = 35/63 (55.56%), Postives = 47/63 (74.60%), Query Frame = 1 
Query:   28 LDARLTDLGKQQCATLKATKHGIEKEAQLVVVSPLTRAIQTATLTIDQVEG-VPWVALECVRE 213
            +DARLTDLG++QC TL+       +E QL+VVSP++RAI TA +  D+V   VPW+ALEC+RE
Sbjct:   62 MDARLTDLGREQCRTLQGRVVSNCEEVQLMVVSPMSRAIDTAMIAFDKVPSHVPWIALECIRE 124
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BLAST of mRNA_E-siliculosus-1a_F_contig12350.1470.1 vs. uniprot
Match: A0A836CMC3_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CMC3_9STRA)

HSP 1 Score: 70.1 bits (170), Expect = 6.800e-13
Identity = 36/64 (56.25%), Postives = 47/64 (73.44%), Query Frame = 1 
Query:   25 HLDARLTDLGKQQCATLKATKHGIEKEAQLVVVSPLTRAIQTATLTIDQVEG-VPWVALECVRE 213
            HLDARLTDLG++Q   L AT      E QL+VVSP++RA++TA++  +QV   +PWVALEC RE
Sbjct:   66 HLDARLTDLGREQAQGLGATLGTTIDEIQLMVVSPMSRALETASIAFEQVPSHIPWVALECARE 129
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BLAST of mRNA_E-siliculosus-1a_F_contig12350.1470.1 vs. uniprot
Match: A0A7S2Y325_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2Y325_9STRA)

HSP 1 Score: 67.8 bits (164), Expect = 7.440e-12
Identity = 36/64 (56.25%), Postives = 46/64 (71.88%), Query Frame = 1 
Query:   31 DARLTDLGKQQCATLKATK---HGIEKEAQLVVVSPLTRAIQTATLTIDQVEGVPWVALECVRE 213
            DARLT+ GK+QC      +   H + K  QLV+VSPLTR ++TA L +D+V GVPWVALEC+RE
Sbjct:   93 DARLTEHGKRQCREANEAEKLNHHL-KGHQLVLVSPLTRCLETAELVVDKVAGVPWVALECIRE 155
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BLAST of mRNA_E-siliculosus-1a_F_contig12350.1470.1 vs. uniprot
Match: A0A7S3HBU0_9STRA (Hypothetical protein (Fragment) n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3HBU0_9STRA)

HSP 1 Score: 61.2 bits (147), Expect = 8.480e-10
Identity = 31/62 (50.00%), Postives = 43/62 (69.35%), Query Frame = 1 
Query:   31 DARLTDLGKQQCATLKATKHGIEKEAQLVVVSPLTRAIQTATLTIDQVEG-VPWVALECVRE 213
            DA LT+LG +QC   + +      +AQL+VVSPL R +QTAT    Q++G +PWVA+EC+RE
Sbjct:   89 DATLTELGLEQCKNFRESNTNSFADAQLLVVSPLRRTMQTATHCFPQLKGNIPWVAVECLRE 150
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BLAST of mRNA_E-siliculosus-1a_F_contig12350.1470.1 vs. uniprot
Match: A0A6V1S4P3_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1S4P3_HETAK)

HSP 1 Score: 62.0 bits (149), Expect = 9.690e-10
Identity = 40/68 (58.82%), Postives = 44/68 (64.71%), Query Frame = 1 
Query:   31 DARLTDLGKQQCATLKAT------KHGIEKEAQLVVVSPLTRAIQTATLTIDQVEG-VPWVALECVRE 213
            DA LT LGK+QCAT KA       +HG     +LVVVSPL R +QTATL   Q  G VPWVALE VRE
Sbjct:   65 DAELTALGKEQCATAKAEWASARGRHGYGPP-ELVVVSPLRRCLQTATLAFGQYAGQVPWVALEEVRE 131
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BLAST of mRNA_E-siliculosus-1a_F_contig12350.1470.1 vs. uniprot
Match: A0A4D9DAJ2_9STRA (Uncharacterized protein n=3 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9DAJ2_9STRA)

HSP 1 Score: 60.1 bits (144), Expect = 5.170e-9
Identity = 31/62 (50.00%), Postives = 37/62 (59.68%), Query Frame = 1 
Query:   28 LDARLTDLGKQQCATLKATKHGIEKEAQLVVVSPLTRAIQTATLTIDQVEGVPWVALECVRE 213
            LD  LT LG  QC  LK +   I+    LVV+SPL RA+ TA L  D  + V W+ALE VRE
Sbjct:  107 LDPELTKLGVSQCLALKPSVEAIQSSIDLVVISPLRRALMTAALAFDHRKEVSWIALETVRE 168
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BLAST of mRNA_E-siliculosus-1a_F_contig12350.1470.1 vs. uniprot
Match: A0A6T6L9S4_9RHOD (Hypothetical protein n=2 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A6T6L9S4_9RHOD)

HSP 1 Score: 53.5 bits (127), Expect = 1.070e-6
Identity = 31/64 (48.44%), Postives = 45/64 (70.31%), Query Frame = 1 
Query:   22 EHLDARLTDLGKQQCATLKATKHGIEKEAQLVVVSPLTRAIQTATLTIDQVEGVPWVALECVRE 213
            EH+DARLTDLG++Q ++L  ++ G+  E  L++VSPLTR +QTATL   +   +P V  E +RE
Sbjct:   75 EHVDARLTDLGREQASSL--SERGLVPE--LILVSPLTRTLQTATLAFPE-NKIPMVVKEDIRE 133
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BLAST of mRNA_E-siliculosus-1a_F_contig12350.1470.1 vs. uniprot
Match: A0A482S1J1_9ARCH (Uncharacterized protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482S1J1_9ARCH)

HSP 1 Score: 52.4 bits (124), Expect = 2.980e-6
Identity = 24/62 (38.71%), Postives = 41/62 (66.13%), Query Frame = 1 
Query:   31 DARLTDLGKQQCATLKATKHGIEKEAQLVVVSPLTRAIQTATLTIDQVEG-VPWVALECVRE 213
            DA +T+ G +QC  L      +   +QL+VVSP++R +QTA+ +   ++  +PWV++EC+RE
Sbjct:   76 DAHITEEGLKQCQELSEKMKHVTAHSQLLVVSPMSRTLQTASHSFPHLQHKIPWVSIECIRE 137
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig12350.1470.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 18
Match NameE-valueIdentityDescription
D8LI64_ECTSI1.410e-3498.44Catalytic, putative n=2 Tax=Ectocarpus TaxID=2879 ... [more]
D8LI65_ECTSI8.210e-3189.06Catalytic, putative n=2 Tax=Ectocarpus TaxID=2879 ... [more]
A0A835YRM9_9STRA4.540e-1355.56Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A836CMC3_9STRA6.800e-1356.25Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A7S2Y325_9STRA7.440e-1256.25Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A7S3HBU0_9STRA8.480e-1050.00Hypothetical protein (Fragment) n=1 Tax=Spumella e... [more]
A0A6V1S4P3_HETAK9.690e-1058.82Hypothetical protein n=2 Tax=Heterosigma akashiwo ... [more]
A0A4D9DAJ2_9STRA5.170e-950.00Uncharacterized protein n=3 Tax=Monodopsidaceae Ta... [more]
A0A6T6L9S4_9RHOD1.070e-648.44Hypothetical protein n=2 Tax=Rhodosorus marinus Ta... [more]
A0A482S1J1_9ARCH2.980e-638.71Uncharacterized protein n=1 Tax=archaeon TaxID=190... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig12350contigE-siliculosus-1a_F_contig12350:230..1385 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start0
Stop0
Cds size213
Model size213
Exons2
Hectar predicted targeting categoryother localisation
Best eggNOG OGNA|NA|NA
Best tax levelEukaryota
COG Functional cat.S
EggNOG OGsKOG4754@1,KOG4754@2759
EggNOG free text desc.isomerase activity
Seed eggNOG ortholog2880.D8LI64
Seed ortholog evalue5.6e-27
Seed ortholog score126.3
Taxonomic scopeEukaryota
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927755.987692-CDS-E-siliculosus-1a_F_contig12350:229..4001622927755.987692-CDS-E-siliculosus-1a_F_contig12350:229..400Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig12350 230..400 +
1696318667.7283728-CDS-E-siliculosus-1a_F_contig12350:229..4001696318667.7283728-CDS-E-siliculosus-1a_F_contig12350:229..400Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig12350 230..400 +
1622927755.9997742-CDS-E-siliculosus-1a_F_contig12350:1343..13851622927755.9997742-CDS-E-siliculosus-1a_F_contig12350:1343..1385Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig12350 1344..1385 +
1696318667.7408204-CDS-E-siliculosus-1a_F_contig12350:1343..13851696318667.7408204-CDS-E-siliculosus-1a_F_contig12350:1343..1385Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig12350 1344..1385 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig12350.1470.1prot_E-siliculosus-1a_F_contig12350.1470.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig12350 230..1385 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig12350.1470.1

>prot_E-siliculosus-1a_F_contig12350.1470.1 ID=prot_E-siliculosus-1a_F_contig12350.1470.1|Name=mRNA_E-siliculosus-1a_F_contig12350.1470.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=71bp
HTRVRAQEHLDARLTDLGKQQCATLKATKHGIEKEAQLVVVSPLTRAIQT
ATLTIDQVEGVPWVALECVRE
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mRNA from alignment at E-siliculosus-1a_F_contig12350:230..1385+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig12350.1470.1 ID=mRNA_E-siliculosus-1a_F_contig12350.1470.1|Name=mRNA_E-siliculosus-1a_F_contig12350.1470.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=1156bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig12350:230..1385+ (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
CACACACGCGTGCGTGCGCAGGAGCACCTCGACGCCAGGCTTACCGATCT
CGGCAAGCAGCAGTGCGCGACATTGAAGGCGACGAAGCACGGCATCGAAA
AGGAGGCGCAGCTGGTCGTTGTTTCTCCACTTACCAGAGCGATACAGACC
GCCACGTTGACCATTGATCAGGTATGACACCCAACCTTGAGGGCGTACTC
ATGTTTGGCATGCCTTAGCGGTTGAGCCAATAACCACGCATCCTCGAGAG
GACTGAGGGGGGGGGGGTGTACTCATAGTCGTGCATGCCGTAGTCGTCAG
ACCATTGACCTCGCAACCCTCCAAAGCCGGGACGGAGCACGCCGGGTTCT
CACCGGACCAGGCTAGCACATCACCAAGCGCGAAGCCCCGTGACGTATTC
GGCGAGTCGCATGAAGGGTGCGCTGCATCCTTCTAAGGGTGTTCGGCATC
TTGTGCTTACCTCCTTGCATACCGATGATAGCTGCGTACGACTTTATTTA
TTTTCTGGTGTTTTTACTCCCAGAAACAGTTATGAGCACAGTGTAAACTG
CCTCGAGGGTGTTTGACCAATTTTACCACAACGTGGTGCGAGAGGACCAT
GGAGGGCGGGACTATTTTTCCCAGTTGTACCGGCATCGACCGTTTTGTGT
GAATTCAGGCGTGCAAACATTGGTGCTGAAGATGTTTGGGAGAAGTGTCG
CGGGTCGTCATTCTTAGCGCTCCGTCGATCTTACCCAACCCTAGGCCAGA
GAAATGCCAGAGTTGTCTGTCGTGCTCCTTCAACGTATGAGCTTTATTCT
CACCTCTGTAGTTCTTGTCTAAATCAATTTGTCTATAAGATTCTCACCAT
CTATCTTGACCAGAAACCGTGCTTCTCCCCGCCTGGCCTAGATCAAGTAC
CGCAAGTGTGAGTAAGGGGTGCCACGTGTTCGTATATACCCCCAAGTTGG
TATTTCATCTCGTCTTCTACGGATCCGACCGCACACGCACGCAATCACAC
GAACGTCGCGTTGTCTCCACGATCACCCCCCCACACCCCGCGCTCCCCGC
CCCTGCCCCGGGTGTGTCGCCGCTTGGATTGCACCGCACCTACTTTAATA
CTGTCGTTATCCAGGTGGAGGGAGTGCCGTGGGTGGCTCTGGAGTGCGTG
AGGGAG
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig12350:230..1385+

>mRNA_E-siliculosus-1a_F_contig12350.1470.1 ID=mRNA_E-siliculosus-1a_F_contig12350.1470.1|Name=mRNA_E-siliculosus-1a_F_contig12350.1470.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=426bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig12350:230..1385+ (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
CACACACGCGTGCGTGCGCAGGAGCACCTCGACGCCAGGCTTACCGATCT
CGGCAAGCAGCAGTGCGCGACATTGAAGGCGACGAAGCACGGCATCGAAA
AGGAGGCGCAGCTGGTCGTTGTTTCTCCACTTACCAGAGCGATACAGACC
GCCACGTTGACCATTGATCAGCACACACGCGTGCGTGCGCAGGAGCACCT
CGACGCCAGGCTTACCGATCTCGGCAAGCAGCAGTGCGCGACATTGAAGG
CGACGAAGCACGGCATCGAAAAGGAGGCGCAGCTGGTCGTTGTTTCTCCA
CTTACCAGAGCGATACAGACCGCCACGTTGACCATTGATCAGGTGGAGGG
AGTGCCGTGGGTGGCTCTGGAGTGCGTGAGGGAGGTGGAGGGAGTGCCGT
GGGTGGCTCTGGAGTGCGTGAGGGAG
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