mRNA_E-siliculosus-1a_F_contig1234.1464.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig1234.1464.1
Unique NamemRNA_E-siliculosus-1a_F_contig1234.1464.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig1234.1464.1 vs. uniprot
Match: D7FZK7_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FZK7_ECTSI)

HSP 1 Score: 207 bits (528), Expect = 5.310e-65
Identity = 176/189 (93.12%), Postives = 177/189 (93.65%), Query Frame = 1 
Query:   55 MALSLVGDYGGSXXXXXXXXXXXXXXXXXXXXXVSLPSAADLFDSVDTSSASFLSAPATSAADAPLTTPKRKKTDGGGSSNKKNKDSKGGTGSANKGLVPPQMSRPNIVTEDSALWSSDASRKXXXXXXXXXXXXXXXXXXXXXXXXXXXAKELTFKQREKAKRDKGQASRGKSYVEEEKRLLRELGSS 621
            MALSLVGDYGGSXXXXXXXXXXXXXXXXXXXXX   PSAADLFDSVDTSSASFLSAPATSA DAPLTTPKRKKTDG       +KDSKGG GSANKGLVPPQMSRPNIVTEDSALWSSDASRKXXXXXXXXXXXXXXXXXXXXXXXXXXXAKELTFKQREKAKRDKGQASRGKSYVEEEKRLLRELGSS
Sbjct:    1 MALSLVGDYGGSXXXXXXXXXXXXXXXXXXXXXXXXPSAADLFDSVDTSSASFLSAPATSATDAPLTTPKRKKTDGXXXXXXXSKDSKGGAGSANKGLVPPQMSRPNIVTEDSALWSSDASRKXXXXXXXXXXXXXXXXXXXXXXXXXXXAKELTFKQREKAKRDKGQASRGKSYVEEEKRLLRELGSS 189
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BLAST of mRNA_E-siliculosus-1a_F_contig1234.1464.1 vs. uniprot
Match: A0A7S2R5U2_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2R5U2_9STRA)

HSP 1 Score: 72.8 bits (177), Expect = 1.490e-12
Identity = 68/159 (42.77%), Postives = 83/159 (52.20%), Query Frame = 1 
Query:  160 LPSAADLFDSVDTSSASFLS-APATSAADAPLTTPKRKKT----DGGGSSNKKNKDSKGGTGSANKGLVPPQMS-RPNIVTEDSALWSSDASRKXXXXXXXXXXXXXXXXXXXXXXXXXXXAKELTFKQREKAKRDKGQASRGKSYVEEEKRLLRELGS 618
            LPSAA+LF SV    ASFLS A  T A + P    K++KT    D GG S  K + +K      +  LVPPQ+S RPNI TED + WS+  S                              ++L+F QREK KRD G  SRGKS+VEEEKRLLR+  S
Sbjct:   44 LPSAAELFSSVG--EASFLSVASPTPAPEIPPIQAKKRKTATASDVGGGSGDKPRRTK------SSHLVPPQLSSRPNIATEDVSQWSTSKSSAALKKKDK---------------------EKLSFNQREKRKRDNGMQSRGKSFVEEEKRLLRQAES 173
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BLAST of mRNA_E-siliculosus-1a_F_contig1234.1464.1 vs. uniprot
Match: A0A7S2HPJ6_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2HPJ6_9STRA)

HSP 1 Score: 62.8 bits (151), Expect = 8.490e-9
Identity = 60/158 (37.97%), Postives = 78/158 (49.37%), Query Frame = 1 
Query:  154 VSLPSAADLFDSVDTSSASFLSA--PATSAADAPLTTPKRKKTDGGGSSNKKNKDS--KGGTGSANKGLVPPQMSRPNIVTEDSALWSSDASRKXXXXXXXXXXXXXXXXXXXXXXXXXXXAKELTFKQREKAKRDKGQASRGKSYVEEEKRLLRELG 615
            V+LPSA++LF SV+   ASFL+A  P  +   APL  PK+KK        KKN D+          + L+PPQ++RPN+ TED   WS+  S                                 +F  REK KRD G  SR K++VEEEKRLLR+ G
Sbjct:   44 VALPSASELFSSVE--GASFLTAASPVPTPEVAPLQ-PKKKK--------KKNTDAFQPASRRKMREQLLPPQLARPNLSTEDYRQWSTTKSADAHNDSKKKDKSSKDAK---------------SFNSREKRKRDLGMQSRHKNFVEEEKRLLRQAG 175
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BLAST of mRNA_E-siliculosus-1a_F_contig1234.1464.1 vs. uniprot
Match: A0A6V3BDJ3_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V3BDJ3_HETAK)

HSP 1 Score: 58.2 bits (139), Expect = 6.340e-7
Identity = 37/91 (40.66%), Postives = 48/91 (52.75%), Query Frame = 1 
Query:  346 LVPPQMS--RPNIVTEDSALWSSDASRKXXXXXXXXXXXXXXXXXXXXXXXXXXXAKELTFKQREKAKRDKGQASRGKSYVEEEKRLLREL 612
            LVPPQM   RPN VTE + LW+ +                                K  +F+ +EK KRD+GQASRGK+YVEEEKR+LR++
Sbjct:  138 LVPPQMQLKRPNHVTEATELWTKNKQGSKQQD------------------------KNKSFRHKEKLKRDRGQASRGKNYVEEEKRILRQM 204
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BLAST of mRNA_E-siliculosus-1a_F_contig1234.1464.1 vs. uniprot
Match: A0A484NNZ0_9ASTE (Uncharacterized protein n=2 Tax=Cuscuta sect. Cleistogrammica TaxID=1824901 RepID=A0A484NNZ0_9ASTE)

HSP 1 Score: 55.1 bits (131), Expect = 5.150e-6
Identity = 29/36 (80.56%), Postives = 31/36 (86.11%), Query Frame = 1 
Query:  508 KELTFKQREKAKRDKGQASRGKSYVEEEKRLLRELG 615
            K L+F Q+EK KRD GQASRGKSYVEEEKRLLRE G
Sbjct:  130 KNLSFSQKEKRKRDMGQASRGKSYVEEEKRLLRESG 165
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BLAST of mRNA_E-siliculosus-1a_F_contig1234.1464.1 vs. uniprot
Match: A0A444EQ59_ENSVE (Uncharacterized protein n=2 Tax=Ensete ventricosum TaxID=4639 RepID=A0A444EQ59_ENSVE)

HSP 1 Score: 53.5 bits (127), Expect = 7.870e-6
Identity = 28/36 (77.78%), Postives = 31/36 (86.11%), Query Frame = 1 
Query:  508 KELTFKQREKAKRDKGQASRGKSYVEEEKRLLRELG 615
            K L+F Q+EK KRD GQASRGK+YVEEEKRLLRE G
Sbjct:   81 KNLSFSQKEKRKRDLGQASRGKNYVEEEKRLLRESG 116
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BLAST of mRNA_E-siliculosus-1a_F_contig1234.1464.1 vs. uniprot
Match: A0A5A7NWG9_STRAF (UPF0690 protein C1orf52 homolog n=1 Tax=Striga asiatica TaxID=4170 RepID=A0A5A7NWG9_STRAF)

HSP 1 Score: 54.7 bits (130), Expect = 8.090e-6
Identity = 29/37 (78.38%), Postives = 32/37 (86.49%), Query Frame = 1 
Query:  505 AKELTFKQREKAKRDKGQASRGKSYVEEEKRLLRELG 615
            A  L+F+Q+EK KRD GQASRGKSYVEEEKRLLRE G
Sbjct:  138 ANNLSFQQKEKRKRDLGQASRGKSYVEEEKRLLRESG 174
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BLAST of mRNA_E-siliculosus-1a_F_contig1234.1464.1 vs. uniprot
Match: UPI001C4AD994 (UPF0690 protein C1orf52 homolog isoform X1 n=4 Tax=Zingiber officinale TaxID=94328 RepID=UPI001C4AD994)

HSP 1 Score: 54.7 bits (130), Expect = 8.660e-6
Identity = 29/36 (80.56%), Postives = 31/36 (86.11%), Query Frame = 1 
Query:  508 KELTFKQREKAKRDKGQASRGKSYVEEEKRLLRELG 615
            K L+F Q+EK KRD GQASRGKSYVEEEKRLLRE G
Sbjct:  144 KNLSFSQKEKRKRDLGQASRGKSYVEEEKRLLRESG 179
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BLAST of mRNA_E-siliculosus-1a_F_contig1234.1464.1 vs. uniprot
Match: A0A6P6WL81_COFAR (uncharacterized protein LOC113716146 n=2 Tax=Coffea TaxID=13442 RepID=A0A6P6WL81_COFAR)

HSP 1 Score: 54.3 bits (129), Expect = 1.220e-5
Identity = 28/36 (77.78%), Postives = 32/36 (88.89%), Query Frame = 1 
Query:  508 KELTFKQREKAKRDKGQASRGKSYVEEEKRLLRELG 615
            +EL+F Q+EK KRD GQASRGK+YVEEEKRLLRE G
Sbjct:  146 RELSFSQKEKRKRDLGQASRGKNYVEEEKRLLRESG 181
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BLAST of mRNA_E-siliculosus-1a_F_contig1234.1464.1 vs. uniprot
Match: UPI00125D6EC0 (uncharacterized protein LOC116004549 n=2 Tax=Ipomoea TaxID=4119 RepID=UPI00125D6EC0)

HSP 1 Score: 53.9 bits (128), Expect = 1.310e-5
Identity = 28/36 (77.78%), Postives = 31/36 (86.11%), Query Frame = 1 
Query:  508 KELTFKQREKAKRDKGQASRGKSYVEEEKRLLRELG 615
            K L+F Q+EK KRD GQASRGK+YVEEEKRLLRE G
Sbjct:  128 KNLSFSQKEKRKRDMGQASRGKNYVEEEKRLLRESG 163
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig1234.1464.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FZK7_ECTSI5.310e-6593.12Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A7S2R5U2_9STRA1.490e-1242.77Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A7S2HPJ6_9STRA8.490e-937.97Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A6V3BDJ3_HETAK6.340e-740.66Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A484NNZ0_9ASTE5.150e-680.56Uncharacterized protein n=2 Tax=Cuscuta sect. Clei... [more]
A0A444EQ59_ENSVE7.870e-677.78Uncharacterized protein n=2 Tax=Ensete ventricosum... [more]
A0A5A7NWG9_STRAF8.090e-678.38UPF0690 protein C1orf52 homolog n=1 Tax=Striga asi... [more]
UPI001C4AD9948.660e-680.56UPF0690 protein C1orf52 homolog isoform X1 n=4 Tax... [more]
A0A6P6WL81_COFAR1.220e-577.78uncharacterized protein LOC113716146 n=2 Tax=Coffe... [more]
UPI00125D6EC01.310e-577.78uncharacterized protein LOC116004549 n=2 Tax=Ipomo... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig1234contigE-siliculosus-1a_F_contig1234:22857..24831 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start0
Stop1
Cds size624
Model size626
Exons3
Ec32 orthologEc-01_010070.1
Ec32 ortholog descriptionconserved unknown protein
Hectar predicted targeting categoryother localisation
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig1234.1464.1prot_E-siliculosus-1a_F_contig1234.1464.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig1234 22857..24831 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927755.1160102-CDS-E-siliculosus-1a_F_contig1234:22856..229431622927755.1160102-CDS-E-siliculosus-1a_F_contig1234:22856..22943Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1234 22857..22943 -
1696318667.1535764-CDS-E-siliculosus-1a_F_contig1234:22856..229431696318667.1535764-CDS-E-siliculosus-1a_F_contig1234:22856..22943Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1234 22857..22943 -
1622927755.126033-CDS-E-siliculosus-1a_F_contig1234:23495..238791622927755.126033-CDS-E-siliculosus-1a_F_contig1234:23495..23879Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1234 23496..23879 -
1696318667.1774027-CDS-E-siliculosus-1a_F_contig1234:23495..238791696318667.1774027-CDS-E-siliculosus-1a_F_contig1234:23495..23879Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1234 23496..23879 -
1622927755.1356902-CDS-E-siliculosus-1a_F_contig1234:24678..248311622927755.1356902-CDS-E-siliculosus-1a_F_contig1234:24678..24831Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1234 24679..24831 -
1696318667.1967292-CDS-E-siliculosus-1a_F_contig1234:24678..248311696318667.1967292-CDS-E-siliculosus-1a_F_contig1234:24678..24831Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1234 24679..24831 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig1234.1464.1

>prot_E-siliculosus-1a_F_contig1234.1464.1 ID=prot_E-siliculosus-1a_F_contig1234.1464.1|Name=mRNA_E-siliculosus-1a_F_contig1234.1464.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=208bp
QQYAPVPARPYACFPASGMALSLVGDYGGSGSSSEESEEESEEEQKPKKP
QVSLPSAADLFDSVDTSSASFLSAPATSAADAPLTTPKRKKTDGGGSSNK
KNKDSKGGTGSANKGLVPPQMSRPNIVTEDSALWSSDASRKRQRQGEAER
RGAAAAAAGGAGKGKGGGAKELTFKQREKAKRDKGQASRGKSYVEEEKRL
LRELGSS*
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mRNA from alignment at E-siliculosus-1a_F_contig1234:22857..24831-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig1234.1464.1 ID=mRNA_E-siliculosus-1a_F_contig1234.1464.1|Name=mRNA_E-siliculosus-1a_F_contig1234.1464.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=1975bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1234:22857..24831- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
CAGCAGTACGCACCCGTGCCTGCTAGACCCTACGCTTGCTTCCCTGCTTC
AGGTATGGCTCTGTCACTTGTGGGAGACTATGGGGGTTCGGGGTCTTCTT
CCGAGGAGTCAGAGGAGGAGTCAGAGGAGGAGCAAAAGCCGAAGAAACCT
CAGGTATGGCTATACCCCCCCCGTTGCTGAAGTTTTATTTTCAAGTCAAA
CGAGGATAGATCTGGCGCTCTTGACCTCCTGCAGGTTATAACGGGCTTGG
AGCTACAGCTCTCGTGCGCCCTTCCCTCCCCTCCCCCCACTCGTGCTCCT
GGTTTCGAGTACTTGTGAGTACCGCATTGCTGGCAGTCGGGCACGTTGGT
CTACGACAAGAGGTGGAACGCCAGTCTCGTCTCTGCAGCGGTCGGAAAAA
GCGGTTACATTTGCGGCAATTTGATCATACTCATAACCGTGGCGCTGCGC
ATAGTACTCGCTTGACGATCCTGGTGGAAGGCCGGGCAAGGATCCGCAGT
TGACGTACACCTAGATTGGCTGTCTCTCCGTGGGTTTCGTTGCTATGACG
TCTTTCTCGAGAGTGGCACGGTTGGTACGTTGTATACCACCCCCCCTCGC
CGACGAACAGTGTACAACCCTCCTGGTTTTCCCGAGTTAGCACTGGCCCT
TGGTTCGATCACTTAAAACACATCACTCATCATTCTCAACCCCACGGGTG
TTAAAAATCGACATTCTGCGTCCCCCCCCCACTCCTCCAATCAATCCATG
GCGTATCTGGTTTGGCACAGCAATCCCAAAGATGATGACAAGCTTCCGTT
TGATCTTACGCAACGCAACCTGGACCGCCAAAACCCGGCATATCTCTCCG
GCTCGGCGTTGCAGGGATTCGAGGTCGATGGTCTAACGGCTGCGGCATGC
GTTTACACGAGTACACACACATACACACACACACACACACACCCTCTCGC
AGGTCTCCCTCCCGTCAGCCGCGGATCTGTTCGACAGCGTAGACACGAGC
TCCGCTTCCTTCCTGTCCGCCCCGGCGACATCAGCGGCAGACGCCCCGCT
CACAACACCCAAGCGCAAGAAAACAGATGGCGGCGGCAGTAGCAACAAGA
AGAACAAGGACTCCAAGGGGGGGACGGGGAGCGCGAACAAGGGCCTGGTT
CCCCCGCAGATGTCTCGGCCGAACATCGTCACGGAGGACTCTGCATTGTG
GTCTTCGGACGCCTCGAGGAAGCGGCAACGGCAGGGCGAAGCGGAGAGGA
GAGGGGCCGCCGCCGCGGCTGCGGGGGGAGCGGGCAAGGGGAAAGGTGGG
GGCGCGAAAGAGCTGACGTTCAAGCAGAGAGAGAAGGTAAGCGGCGCTGG
GTGGGGTTCTCCCGTTTTTGTAGGCAAGTGTCGGGGGTCTGCCCCCCCCC
CCCCTTCTTTTTGTTTTACGGGATTGGTGCCTTTCTTTGGGATAAGACAA
CCAAGCCGGTACCTGGGTAGCAGCTGCAAGTTCGTCCTCTTTCGTGCTAC
AGCACTCGTGCACGTGTACTGTCAGCGCACCGGCGATCTCCGTCCAGTGC
CACATTCTTCCACCACGAAAACTCTCGTCTGCCCTAGCACCGAAGCTTAT
TTGCGCTGTGCCAAGCCATGTTCCAGTTTGCCAGGCACCAACCTGAAGAA
GCTGTTTTTTCTTTGTTGCCAGCGTGGAGTTCGTTTTTCTCAGGGCTCAG
GTTTTGGAGTTGCGGCCAAAATGCAACAATGAACAATAGACGGAGAGATA
CCATGCGCGGTCTACAGCAGTCGGGCGAGCGGACCAGTAACCCCCGAAAG
ATTTCGAGTCGACTTATTATGTAGGCCCTTACGTTCTGTCAAGTAAATGT
TCTTTCTTGTGCTGTGTCCGCGTCTTCGAAACCGTCAGGCAAAGCGAGAC
AAGGGGCAGGCGTCGCGAGGCAAGAGCTACGTTGAGGAGGAGAAGCGGTT
GCTGAGAGAACTCGGCTCGTCATAG
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig1234:22857..24831-

>mRNA_E-siliculosus-1a_F_contig1234.1464.1 ID=mRNA_E-siliculosus-1a_F_contig1234.1464.1|Name=mRNA_E-siliculosus-1a_F_contig1234.1464.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=1248bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1234:22857..24831- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
CAGCAGTACGCACCCGTGCCTGCTAGACCCTACGCTTGCTTCCCTGCTTC
AGGTATGGCTCTGTCACTTGTGGGAGACTATGGGGGTTCGGGGTCTTCTT
CCGAGGAGTCAGAGGAGGAGTCAGAGGAGGAGCAAAAGCCGAAGAAACCT
CAGCAGCAGTACGCACCCGTGCCTGCTAGACCCTACGCTTGCTTCCCTGC
TTCAGGTATGGCTCTGTCACTTGTGGGAGACTATGGGGGTTCGGGGTCTT
CTTCCGAGGAGTCAGAGGAGGAGTCAGAGGAGGAGCAAAAGCCGAAGAAA
CCTCAGGTCTCCCTCCCGTCAGCCGCGGATCTGTTCGACAGCGTAGACAC
GAGCTCCGCTTCCTTCCTGTCCGCCCCGGCGACATCAGCGGCAGACGCCC
CGCTCACAACACCCAAGCGCAAGAAAACAGATGGCGGCGGCAGTAGCAAC
AAGAAGAACAAGGACTCCAAGGGGGGGACGGGGAGCGCGAACAAGGGCCT
GGTTCCCCCGCAGATGTCTCGGCCGAACATCGTCACGGAGGACTCTGCAT
TGTGGTCTTCGGACGCCTCGAGGAAGCGGCAACGGCAGGGCGAAGCGGAG
AGGAGAGGGGCCGCCGCCGCGGCTGCGGGGGGAGCGGGCAAGGGGAAAGG
TGGGGGCGCGAAAGAGCTGACGTTCAAGCAGAGAGAGAAGGTCTCCCTCC
CGTCAGCCGCGGATCTGTTCGACAGCGTAGACACGAGCTCCGCTTCCTTC
CTGTCCGCCCCGGCGACATCAGCGGCAGACGCCCCGCTCACAACACCCAA
GCGCAAGAAAACAGATGGCGGCGGCAGTAGCAACAAGAAGAACAAGGACT
CCAAGGGGGGGACGGGGAGCGCGAACAAGGGCCTGGTTCCCCCGCAGATG
TCTCGGCCGAACATCGTCACGGAGGACTCTGCATTGTGGTCTTCGGACGC
CTCGAGGAAGCGGCAACGGCAGGGCGAAGCGGAGAGGAGAGGGGCCGCCG
CCGCGGCTGCGGGGGGAGCGGGCAAGGGGAAAGGTGGGGGCGCGAAAGAG
CTGACGTTCAAGCAGAGAGAGAAGGCAAAGCGAGACAAGGGGCAGGCGTC
GCGAGGCAAGAGCTACGTTGAGGAGGAGAAGCGGTTGCTGAGAGAACTCG
GCTCGTCATAGGCAAAGCGAGACAAGGGGCAGGCGTCGCGAGGCAAGAGC
TACGTTGAGGAGGAGAAGCGGTTGCTGAGAGAACTCGGCTCGTCATAG
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