mRNA_E-siliculosus-1a_F_contig122.1389.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

BLAST of mRNA_E-siliculosus-1a_F_contig122.1389.1 vs. uniprot
Match: D8LNX3_ECTSI (Tubulin folding cofactor D n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNX3_ECTSI)

HSP 1 Score: 2553 bits (6617), Expect = 0.000e+0
Identity = 1374/1449 (94.82%), Postives = 1385/1449 (95.58%), Query Frame = 2

Query:  101 MGMLQQLVATDLDLAGREGILKAVKGALEKYLEQPHLLDPHLEDMMAVVMGKAKELVVEREEQVLAESGGGSRGRPGEAFPFQVFICPQLHSMFCVIYQLCRVRGAKAVVRLMPHEAADLEPVIHALQAQDTDDYSTWETRYGLLLWLSMLSLVPFDIDTIDSGLRDSVPPGAGANEDGTKGVVAPVAGVGERQADLVGTILALGMRHLGDAGPTRDAAAACLSSLLTRPDMEAAHLRRFLEWSASVLERVTAEGRAGEEGLSKLAFLVMGVMATLAALFKQGHREKLIDLIPVVFYPVVGLAELGPGQTLLRKMLVKLLQRVGTTYVPPRVVAWRYRRGQRSLLQNLESTPDAASDGGDCCQDEQQAPSVEEKPLDRQEGETVEAGGGEGDAEESSAGGVGVAQDGPPAQVKVDATEVDVIAEEEE-VDVPEELEDIVEALLCGLRDRDTVVRWSAAKGIGRITERLPRELADDVVCSVLDLFVEAEWDGAWHGGCLALAELARRGLLLPERLPRACPLVRRALAYDVRRGAGGVGAHVRDAACYVCWAFARAYSPRVLGPHLPGLCDSMLSTALFDREVNVRRAAAAALQENVGRQGQQHHSGEDDGSGWTDEGGGGVAHGIYIITAADYFSLGNRQQAYLDIGKTVAAFDRYRHHIVDTLRLDKLKHWDPELRRLSALALGRIASLEPAYAAETVLPAALGDTLSPDLLRRHGACLALAEVTLSLGQVSYALPAETVAGLVGIVPALEKARLYRGRGGELMRQAACRLVECLALTKSEVAIKTQLRLLDSVDESLRHAVESVQLSAAAALKAVLENWFPVGRDGPSERLRTRTIGLYVGGLRTEENAAVARGYAMALGVLPRKLAGASPAVLGEVLDALKDVALGRHGPSRSPSPAATGGGXXXXXXXXXXXXXIGGPGGEPDAETRRNAVRSLVTLCEEVGVGRQQQXXXXXXXSRDPGGGEGRQGSGGVSGGRDGVDAREGESGVPAAAACPAWLTLADVEGVLATLLGSSEDYSVDKRGDVGSWCRVEALAGMERLLRLAARASRGLPLANRRGNASVQPPAQQAPETFILPSLEDRLALLDQPPAAMPTPHPCDEVYLTPSMVERVMCAVLKQLSEKLDSVRERAGTVLQKLVQSDDPQLPFVPERAVVKHAITHGAATPAAPGAAGEEGKEAVNWAVPATTFPMVVGLLAVAEYHDAIVEGLVISVGGLSESVVTHSSKTLLRWLRACKSARNLRAVAGLALRLVGMFGDAKGNPRIVVPLLKTLELLLSNECFDSLPAEEHPFAELLLVAVVAEMKGCRDVRKICLGASVFVCMLNYSDPVRPKALRNSVLFLGHRFPNVRKTVAEAMYLKLLANEDVVDEAVYEEALDILTCTAWDGDLQTAKAARSALAQLLGVNVPTA--NKKAGAGTGDAAGEGSGNPRKADEMESYESLVRTVGY 4438
            MGMLQQLVATDLDLAGREG LKAVKGALEKYLEQPHLLDPHLEDMMAVVMG+AKELVVEREEQVLAESGGGSRGRPGEAFPFQVFICPQLHSMFCVIYQLCRVRGAKAVVRLMPHEAADLEPVIHALQAQDTDDYSTWETRYGLLLWLSMLSLVPFDIDTIDSGLRDSVPPGAGANEDG KGVVAPVAGVGERQADLVGTILALGMRHLGDAGPTRDAAA CLSSLLTRPDMEAAHLRRFLEWSASVLERVTAEGRAGEEGLSKLAFLVMGVMATLAALFKQGHREKLIDLIPVVFYPVVGLAELGPGQTLLRKMLVKLLQRVGTTYVPPRVVAWRYRRGQRSLLQNLESTPDAASDGGDCCQDEQQA SVEEKPL+RQEGETVEAGGGEGDAEESSAGGVGVAQDGPPAQVKVDATEVDV+AEEEE VDVPEELEDIVEALLCGLRDRDTVVRWSAAKGIGRITERLPRELADDVVCSVLDLFVEAEWDGAWHGGCLALAELARRGLLLPERLPRACPLVRRALAYDVRRGAGGVGAHVRDAACYVCWAFARAYSPRVLGPHLPGLCDSMLSTALFDREVNVRRAAAAALQENVGRQGQQ  SGEDDG         GVAHGI IITAADYFSLGNRQQAYL+IGKTVAAFDRYRHHIVDTLRLDKLKHWDPELRRLSALALGRIASLEPAYAAETVLPAALGDTLSPDLLRRHGACLALAEVTLSLGQVSYALPAETVAGLVGIVPALEKARLYRGRGGELMRQAACRLVECLALTKSEVAIKTQLRLLDSVDESLRHAVESVQLSAAAALKAVLENWFPVGRDGPSERLRTRTIGLYVGGLRTEENAAVARGYAMALGVLPRKLAGASPAVLGE+LDALK+ ALGRH P RSPS A TG  XXXXXXXXXXXXXIGGPGGEPDAETRRNAVRSLVTLCEEVGVGRQ  XXXXXXX                     GVDAREGESG   AA CPAWLTLADVEGVLATLL SSEDYSVDKRGDVGSWCRVEALAGMERLLRLAARASRGLPLANRRG ASVQPPAQQAPET ILPSL+DRLALLDQPPA++PTPHPCDEVYLTPSMVERVMCAVLKQLSEKLDSVRERAGTVLQKLVQSDDPQLPFVPERA VKHAITHGAATPA PGAAGEEGKE VNWAVPATTFPMVVGLLAVAEYHDAIVEGLV+SVGGLSESVVTHSSK LLRWLRACKSARNLRAVAGLALRLVGMFGDAKGNPRIVVPLLKTLELLLSNECFDSLPAE HPFAELLLVAVVAEMKGCRDVRKICLGASVFVCMLNYSDPVRPKALRNSVLFLGHRFPNVRKTVAEAMYLKLLANE+VVDEAVYEEALDILTCTAWDGDLQTAKAARSALA LLGVNVPTA  NKKAGAGTG AAGEG GNPRKADEMESYESLVRTVGY
Sbjct:    1 MGMLQQLVATDLDLAGREGTLKAVKGALEKYLEQPHLLDPHLEDMMAVVMGRAKELVVEREEQVLAESGGGSRGRPGEAFPFQVFICPQLHSMFCVIYQLCRVRGAKAVVRLMPHEAADLEPVIHALQAQDTDDYSTWETRYGLLLWLSMLSLVPFDIDTIDSGLRDSVPPGAGANEDGAKGVVAPVAGVGERQADLVGTILALGMRHLGDAGPTRDAAAGCLSSLLTRPDMEAAHLRRFLEWSASVLERVTAEGRAGEEGLSKLAFLVMGVMATLAALFKQGHREKLIDLIPVVFYPVVGLAELGPGQTLLRKMLVKLLQRVGTTYVPPRVVAWRYRRGQRSLLQNLESTPDAASDGGDCCQDEQQAASVEEKPLERQEGETVEAGGGEGDAEESSAGGVGVAQDGPPAQVKVDATEVDVVAEEEEEVDVPEELEDIVEALLCGLRDRDTVVRWSAAKGIGRITERLPRELADDVVCSVLDLFVEAEWDGAWHGGCLALAELARRGLLLPERLPRACPLVRRALAYDVRRGAGGVGAHVRDAACYVCWAFARAYSPRVLGPHLPGLCDSMLSTALFDREVNVRRAAAAALQENVGRQGQQQ-SGEDDGXXXXXXXXXGVAHGIDIITAADYFSLGNRQQAYLNIGKTVAAFDRYRHHIVDTLRLDKLKHWDPELRRLSALALGRIASLEPAYAAETVLPAALGDTLSPDLLRRHGACLALAEVTLSLGQVSYALPAETVAGLVGIVPALEKARLYRGRGGELMRQAACRLVECLALTKSEVAIKTQLRLLDSVDESLRHAVESVQLSAAAALKAVLENWFPVGRDGPSERLRTRTIGLYVGGLRTEENAAVARGYAMALGVLPRKLAGASPAVLGELLDALKNAALGRHAPPRSPSLATTGXXXXXXXXXXXXXXXIGGPGGEPDAETRRNAVRSLVTLCEEVGVGRQXXXXXXXXXXXXXXXXXXXXXXXXXXX-XXGVDAREGESG---AAWCPAWLTLADVEGVLATLLASSEDYSVDKRGDVGSWCRVEALAGMERLLRLAARASRGLPLANRRGIASVQPPAQQAPETLILPSLQDRLALLDQPPASVPTPHPCDEVYLTPSMVERVMCAVLKQLSEKLDSVRERAGTVLQKLVQSDDPQLPFVPERAAVKHAITHGAATPAVPGAAGEEGKEPVNWAVPATTFPMVVGLLAVAEYHDAIVEGLVMSVGGLSESVVTHSSKALLRWLRACKSARNLRAVAGLALRLVGMFGDAKGNPRIVVPLLKTLELLLSNECFDSLPAE-HPFAELLLVAVVAEMKGCRDVRKICLGASVFVCMLNYSDPVRPKALRNSVLFLGHRFPNVRKTVAEAMYLKLLANEEVVDEAVYEEALDILTCTAWDGDLQTAKAARSALAHLLGVNVPTAAANKKAGAGTGAAAGEGGGNPRKADEMESYESLVRTVGY 1443          

BLAST of mRNA_E-siliculosus-1a_F_contig122.1389.1 vs. uniprot
Match: A0A6H5KH41_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KH41_9PHAE)

HSP 1 Score: 1612 bits (4175), Expect = 0.000e+0
Identity = 873/988 (88.36%), Postives = 889/988 (89.98%), Query Frame = 2

Query:  377 MFCVIYQLCRVRGAKAVVRLMPHEAADLEPVIHALQAQDTDDYSTWETRYGLLLWLSMLSLVPFDIDTIDSGLRDSVPPGAGANE---DGTKGVVAPVAGVGERQADLVGTILALGMRHLGDAGPTRDAAAACLSSLLTRPDMEAAHLRRFLEWSASVLERVTAEGRAGEEGLSKLAFLVMGVMATLAALFKQGHREKLIDLIPVVFYPVVGLAELGPGQTLLRKMLVKLLQRVGTTYVPPRVVAWRYRRGQRSLLQNLESTPDAASDGGDCCQDEQQAPSVEEKPLDRQEGETVEAGGGEGDAEESSAGGVGVAQDGPPAQVKVDATEVDVIAEEE-EVDVPEELEDIVEALLCGLRDRDTVVRWSAAKGIGRITERLPRELADDVVCSVLDLFVEAEWDGAWHGGCLALAELARRGLLLPERLPRACPLVRRALAYDVRRGAGGVGAHVRDAACYVCWAFARAYSPRVLGPHLPGLCDSMLSTALFDREVNVRRAAAAALQENVGRQGQQHHSGEDDGSGWTDEGGGGVAHGIYIITAADYFSLGNRQQAYLDIGKTVAAFDRYRHHIVDTLRLDKLKHWDPELRRLSALALGRIASLEPAYAAETVLPAALGDTLSPDLLRRHGACLALAEVTLSLGQVSYALPAETVAGLVGIVPALEKARLYRGRGGELMRQAACRLVECLALTKSEVAIKTQLRLLDSVDESLRHAVESVQLSAAAALKAVLENWFPVGRDGPSERLRTRTIGLYVGGLRTEENAAVARGYAMALGVLPRKLAGASPAVLGEVLDALKDVALGRHGPSRSPSPAATGGGXXXXXXXXXXXXXIGGPGGEPDAETRRNAVRSLVTLCEEVGVGRQQQXXXXXXX-----SRDPGGGEGRQGSGGVSGGRDGVDAREGESGVPAAAACPAWLTLADVEGVLATLLGSSEDYSVDKRGDVGSWCRVEALAGMERLLRLAARASRGLPLANRRGNASVQPPAQQAP 3313
            MFC+IYQLCR+RGAKAV RLMPHEAADLEPVIHALQAQDTDDYSTWETRYGLLLWLSMLSLVPFDIDTIDS LRDSVPPG GANE   DG KGVVAPVAGVGERQADLVGTILALG+RHLGDAGPTRDAAAACLSSLLTRPDMEAAHLRRFLEWSASVLERVTAEGRAGEEGLSKLAFLVMGVMATLAALFKQGHREKLIDLIPVVFYPVVGLAE+GPGQTLLRKMLVKLLQRVGTTYVPPRV+AWRYRRGQRSLLQ                     A SVEEKPLDRQEG+TVE  G  GDAEE S  GVGVA+DG  AQ K D  EVD +AEEE EV VPEELEDIVEALLCGLRDRDTVVRWSAAKGIGRITERLPRELADDVVCSVLDLFVEAEWDGAWHGGCLALAELARRGLLLPERLPRACPLVRRALAYDVRRGAGGVGAHVRDAACYVCWAFARAYSPRVLGPHLPGLCDSMLSTALFDREVNVRRAAAAALQENVGRQGQQ  SGE DGSGW+D GGGGVAHGI IITAA+YFSLGNRQQAYLDIGK VAAFDRYRHHIVDTLRLDKLKHWDPELRRLSALALGRIA LEPAYAAETVL AALGDTLSPDLLRRHGACLALAEVTLSLGQVSYALPAETVAGLV IVPALEKARLYRGRGGELMRQAACRLVECLAL KSEV+IK+QLRLLDSVDESLRHAVESVQLSAAAALKAVLENWFPVGRDGPSERLRTRTIGLYVGGLRT+ENAAVARGYAMALGVLPRKLAGASPAVLGEVLDALKD ALGRHGP RSPSPA TGGG             IGGPGGEPDAETRRNAVRSLVTLCEEVGVGRQQQXXXXXXX     S++PG GEGR  SGGVS G  GVDA EGESG  AAA CPAWLTLADVEGVLATLL SSEDYSVDKRGDVGSWCRVEAL GMERLLRLAARASRGLPLANR      +     AP
Sbjct:    1 MFCMIYQLCRLRGAKAVARLMPHEAADLEPVIHALQAQDTDDYSTWETRYGLLLWLSMLSLVPFDIDTIDSSLRDSVPPGGGANEEGADGAKGVVAPVAGVGERQADLVGTILALGIRHLGDAGPTRDAAAACLSSLLTRPDMEAAHLRRFLEWSASVLERVTAEGRAGEEGLSKLAFLVMGVMATLAALFKQGHREKLIDLIPVVFYPVVGLAEVGPGQTLLRKMLVKLLQRVGTTYVPPRVIAWRYRRGQRSLLQ---------------------AASVEEKPLDRQEGQTVETNGSGGDAEEISTKGVGVARDGASAQEKADVNEVDAVAEEEDEVGVPEELEDIVEALLCGLRDRDTVVRWSAAKGIGRITERLPRELADDVVCSVLDLFVEAEWDGAWHGGCLALAELARRGLLLPERLPRACPLVRRALAYDVRRGAGGVGAHVRDAACYVCWAFARAYSPRVLGPHLPGLCDSMLSTALFDREVNVRRAAAAALQENVGRQGQQQ-SGEHDGSGWSDGGGGGVAHGIDIITAAEYFSLGNRQQAYLDIGKNVAAFDRYRHHIVDTLRLDKLKHWDPELRRLSALALGRIAPLEPAYAAETVLSAALGDTLSPDLLRRHGACLALAEVTLSLGQVSYALPAETVAGLVSIVPALEKARLYRGRGGELMRQAACRLVECLALAKSEVSIKSQLRLLDSVDESLRHAVESVQLSAAAALKAVLENWFPVGRDGPSERLRTRTIGLYVGGLRTQENAAVARGYAMALGVLPRKLAGASPAVLGEVLDALKDAALGRHGPPRSPSPATTGGGAANGTTETNGGGDIGGPGGEPDAETRRNAVRSLVTLCEEVGVGRQQQXXXXXXXXXXXXSQEPGSGEGRHDSGGVSSGGSGVDAPEGESGA-AAAWCPAWLTLADVEGVLATLLASSEDYSVDKRGDVGSWCRVEALEGMERLLRLAARASRGLPLANRESGGKEEDVVVSAP 965          

BLAST of mRNA_E-siliculosus-1a_F_contig122.1389.1 vs. uniprot
Match: A0A835ZJS0_9STRA (Tubulin folding cofactor D n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZJS0_9STRA)

HSP 1 Score: 1001 bits (2589), Expect = 0.000e+0
Identity = 659/1546 (42.63%), Postives = 829/1546 (53.62%), Query Frame = 2

Query:   65 CERILFAEREQVMGMLQQLVATDLDLAGREGILKAVKGALEKYLEQPHLLDPHLEDMMAVVMGKAKELVVEREEQVLAESGGGSRGRPGEAFPFQVFICPQLHSMFCVIYQLCRVRGAKAVVRLMPHEAADLEPVIHALQAQDTDDYSTWETRYGLLLWLSMLSLVPFDIDTIDSGLRDSVPPGAGANEDGTKGVVAPVAGVGERQADLVGTILALGMRHLGDAGPTRDAAAACLSSLLTRPDMEAAHLRRFLEWSASVLERVTAEGRAGEEGLSKLAFLVMGVMATLAALFKQGHREKLIDLIPVVFYPVVGLAELGPGQTLLRKMLVKLLQRVGTTYVPPRVVAWRYRRGQRSLLQNLESTPDAASDGGDCCQDEQQAPSVEEKPLDRQEGETVEAGGGEGDAEESSAGGVGVAQDGPPAQVKVDATEVDVIAEEEEVDVPEELEDIVEALLCGLRDRDTVVRWSAAKGIGRITERLPRELADDVVCSVLDLFVEAEWDGAWHGGCLALAELARRGLLLPERLPRACPLVRRALAYDVRRGAGGVGAHVRDAACYVCWAFARAYSPRVLGPHLPGLCDSMLSTALFDREVNVRRAAAAALQENVGRQGQQHHSGEDDGSGWTDEGGGGVAHGIYIITAADYFSLGNRQQAYLDIGKTVAAFDRYRHHIVDTLRLDKLKHWDPELRRLSALALGRIASLEPAYAAETVLPAALGDTLSPDLLRRHGACLALAEVTLSLGQVSYA---------------LPAETVAGLVGIVPALEKARLYRGRGGELMRQAACRLVECLALTKS-EVAIKTQLRLLDSVDESLRHAVESVQLSAAAALKAVLENWFPVGRDGPSERLRTRTIGLYVGGLRTEENAAVARGYAMALGVLPRKLAGASPAVLGEVLDALKDVALGRHGPSRSPSPAATGGGXXXXXXXXXXXXXIGGPGGEPDAETRRNAVRSLVTLCEEVGVGRQQQXXXXXXXSRDPGGGEGRQGSGGVSGGRDGVDAREGESGVPAAAACPAWLTLADVEGVLATLLGSSEDYSVDKRGDVGSWCRVEALAGMERLLRLAARASRGLPLANRRGNASVQPPAQQAPETFILPSLEDRLALLDQ---------PPAAMPTPHPCDEVYLTPSMVERVMCAVLKQLSEKLDSVRERAGTVLQKLVQSDDPQLPFVPERAVVKHAI-THGAATPAAPGAAGEEGKEAVNWAVPATTFPMVVGLLAVAEYHDAIVEGLVISVGGLSESVVTHSSKTLLRWLRACKSARNLRAVAGLALRLVGMFGDAKG--------------------------NPRIVVPLLKTLELLLSNECFDSLPAEEHP-FAELLLVAVVAEMKGCRDVRKICLGASVFVCMLNYSDPVRPKALRNSVLFLGHRFPNVRKTVAEAMYLKLLANEDVVD-----------------------------------EAVYEEALDILTCTAWDGDLQTAKAARSALAQLLGVNVPTANKKAGAGTGDAAGEGSGNPRKADEMESYESLVRTVGY 4438
            C  IL ++  +++G L    A D D   R   ++ V+  LEKY+EQ +LLDPHLE +M+V+MG+ K ++   EE    E  G  R               QLH++   +YQLCRVRGAK V+RL+PH+AADLEP + A Q QD  D +TW +RY LLLWL+ML LVPFDI TIDS    +  P A A            A            I AL + HL DA  TRDAAA CL++LL+RPDM+ A L  F+ ++ + LER  A G A EE     AF V G +ATLAALFK+G R  L+ +   +  P+  +A  G  QTLLRKM+VKL QR+G  ++PPRVVAWRY+RGQRSLLQNL                               EG                                         A++ E +V EE+E++VE LLCGLRDRDTVVRWSAAKG+GR+T RLP  LAD+VV SVLDLF +AE DG WHGGCLALAELARRGLLLP+RLP A P+V RA+AYDVRRG  GVGAHVRDAACYVCWAFARAYSP  L PH+  LC +ML TALFDRE+NVRRAAAAA QENVGRQG Q+                 VAHGI ++TAADYF+LG+R  AYL +   VA FD YR  + D  R  +L+HWD ++R  +A  LG +A L+ AYAA+ +LP AL DTLSPDLL RHGACL +AEV L+LG    A               LP +  A +   VPALE+A LYRGRGGE +R AACRL EC AL+ + E+     +RLLD+ DE LR   E VQ +AAAA +A+L      G     E L  R +  +V GL      A ARG+A+ALG LPR LA    A    VL AL          + + +P A                     GGE DAETRRNAV +L  +CE VG G                          VSGG  G                 A LT AD   V A LL +++DY  DKRGDVGSWCRV A+  +ER+ RLA  ASRG+PL      A+  PP        ++P+L  R   +           PP A        E Y TP     V+ A+LKQLSEKLD+VR+ AG  LQ+L+ +  P +P++P R  ++ A+   GAA P              NWA  A  FP +   L++  Y  A+  GLV+SVGGL+ESVV  S+  LL W RA  +A +  AV+ L   LV +                                  R+ +P L+TL+LLL+N+ F  LPA         LL A    ++GCR V K+C GA   + +  + D V   ALR++V  L  RFP+VR+ VAE MY++LLA ED  D                                   EA+ +EAL+IL+CTAWDG L+ A+ AR  +A  LGV +  A  + G+ +G     G     +ADE+  YESLVR VGY
Sbjct:   27 CCSILASQLLELLGRLADPSAADDDALRRSRYVEQVQAILEKYMEQSNLLDPHLEAIMSVIMGRVKAILASEEESGAGEQEGVCRSA-------------QLHALLACVYQLCRVRGAKTVMRLLPHDAADLEPALRAAQRQDRADAATWTSRYALLLWLAMLCLVPFDIATIDSSPVAAAAPHASAE----------AAEXXXXXXGAARAIAALAIAHLSDAAATRDAAAVCLAALLSRPDMDGAPLDGFVAYALAALERA-ARGGAAEEATGA-AFAVRGALATLAALFKRGARAALLPVAQRLLAPLAAIAAQGSSQTLLRKMVVKLFQRIGLAFLPPRVVAWRYQRGQRSLLQNLAGXXXXXXXXXXXX-------XXXXXXXXXXEG-----------------------------------------ADDVEFEVAEEVEEVVEQLLCGLRDRDTVVRWSAAKGVGRVTGRLPLALADEVVGSVLDLFSDAESDGGWHGGCLALAELARRGLLLPQRLPVAVPVVIRAMAYDVRRGTTGVGAHVRDAACYVCWAFARAYSPAALRPHMQELCRAMLCTALFDREINVRRAAAAAFQENVGRQGHQN-----------------VAHGIEVLTAADYFTLGSRAAAYLTVAPYVARFDAYRAALADCARASRLRHWDADVRAAAARCLGALARLDAAYAADVILPQALRDTLSPDLLVRHGACLGVAEVVLALGTPPPASPXXXXXXXXXXXXPLPPQLSAAVADAVPALERANLYRGRGGEAVRAAACRLAECAALSDAVELTAPQLVRLLDTCDECLRQVSEDVQRAAAAAARALLRRACAGGAGAADEALFERAVAAHVRGLAEAAAPAEARGHALALGALPRPLAARDAAARARVLTAL----------AAACAPGAR-------------------VGGEGDAETRRNAVAALAAVCETVGFG--------------------------VSGGGGG-----------------AGLTAADAAAVFAALLRATDDYGTDKRGDVGSWCRVAAMHALERMARLAVAASRGIPLERAPAAAATAPP--------LVPALSGRQPFMQVAVVNGAVAVPPRARRRAAAAAEAYWTPVDAAAVVRALLKQLSEKLDAVRDHAGQALQRLLAARAPAIPYIPLRPQLEAALGVRGAAPPP-------------NWASAADAFPRIARALSLPSYQSAVAAGLVVSVGGLTESVVKQSAAALLAWARAAGAA-DPSAVSQLGATLVALLNGCNAAAXXXXXXXXXXXXXXXXXXXXGSGDRDRVALPALRTLDLLLANDAFAPLPAPARARLGAELLAAARGALRGCRFVAKLCAGAGALMGLAAWPDGVGRGALRSAVALLAQRFPHVRRHVAELMYVRLLAWEDAEDGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXALSPEAL-DEALEILSCTAWDGALEAAREARDGVAAALGVPLLRAGSRRGSASGGGXXXGGA---EADEL-GYESLVRDVGY 1383          

BLAST of mRNA_E-siliculosus-1a_F_contig122.1389.1 vs. uniprot
Match: A0A4D9D438_9STRA (TFCD_C domain-containing protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9D438_9STRA)

HSP 1 Score: 834 bits (2155), Expect = 6.610e-272
Identity = 611/1591 (38.40%), Postives = 799/1591 (50.22%), Query Frame = 2

Query:   50 DNHATCERILFAEREQVMGMLQQLVATDLDLAGREGILKAVKGALEKYLEQPHLLDPHLEDMMAVVMGKAKELVVEREEQVLAESGGGSRGRPGEAFPFQVFICPQLHSMFCVIYQLCRVRGAKAVVRLMPHEAADLEPVIHALQAQDTDDYSTWETRYGLLLWLSMLSLVPFDIDTIDSGLRDSVPPGAGANEDGTKGVVAPVAGVGERQADLVGTILALGMRHLGDAGPTRDAAAACLSSLLTRPDMEAAHLRRFLEWSASVLE-RVTAEGRAGEE---------GLSKL------AFLVMGVMATLAALFKQGHREKLIDLIPVVFYPVVGLAELGPGQ-----TLLRKMLVKLLQRVGTTYVPPRVVAWRYRRGQRSLLQNLESTPDAASDGGDCCQDEQQAPSVEEKPLDRQEGETVEAGGGEGDAEESSAGGVGVAQDGPPAQVKVDATEVDVIAEEEEVDVPEELEDIVEALLCGLRDRDTVVRWSAAKGIGRITERLPRELADDVVCSVLDLFVEAEWDGAWHGGCLALAELARRGLLLPERLPRACPLVRRALAYDVRRGAGGVGAHVRDAACYVCWAFARAYSPRVLGPHLPGLCDSMLSTALFDREVNVRRAAAAALQENVGRQGQQHHSGEDDGSGWTDEGGGGVAHGIYIITAADYFSLGNRQQAYLDIGKTVAAFDRYRHHIVDTLRLDKLKHWDPELRRLSALALGRIASLEPAYAAETVLPAALGDTLSPDLLRRHGACLALAEVTLSLGQVSYALPAETVAGLVGIVPALEKARLYRGRGGELMRQAACRLVECLALTKSEVAIKTQLRLLDSVDESLRHAVESVQLSAAAALKAVLENWFPVGRDGPSERLRTRTIGLYVGGLRTEENAAVARGYAMALGVLPRKLAGASPAVLGEVLDALKDVALGRHGPSRSPSPAATGGGXXXXXXXXXXXXXIGGPGGEPDAETRRNAVRSLVTLCEEVGVGRQQQXXXXXXXSRDPGGGEGRQGSGGVSGGRDGVDAREGESGVPAAAACPAWLTLADVEGVLATLLGSSEDYSVDKRGDVGSWCRVEALAGMERLLRLAARASRGL-----------------PLA------NRRGNASVQ-------------PPAQQAPETF-------ILP-------SLEDRL--ALLDQPPA-AMPTPHPCD-EV--------YLTPSMVERVMCAVLKQLSEKLDSVRERAGTVLQKLVQSDDPQLPFVPERAVVKHAITHGAATPAAPGAAGEEGKEAVNWAVPATTFPMVVGLLAVAEYHDAIVEGLVISVGGLSESVVTHSSKTLLRWLR----------------ACKSARNLRA-VAGLALRLVGMFGDAKGNPRIVVPLLKTLELLLSNECF--------------DSLPAEEHPFAELLLVAVVAEMKGCRDVRKICLGASVFVCMLN--------YSDPVRPKALRNSVLFLGHRFPNVRKTVAEAMYLKLLANEDVVDEA--VYEEALDILTCTAWDG-DLQTAKAARSALAQLLGVNVPTANKKAGAGTGDAAGEGS---GNPRKADEMESYESLVRTVGY 4438
            +   +CE   F ER++V+  L  L   +   A   G L+ ++  L+KY+EQ  LLDP +E M+A+ MG+A+ +++    + LAE        P  +FPFQ+F   QL ++F  +Y LCR+RG K V+   PHEA DLEPV+HALQ+QD  D   W+TRY LLLWLSML LVPF+I +IDSG+        GA + G            +    LVG I      +L D GPTR+AAA CL+SLL RPDME   L  FL+W+  VL  R  + G   EE         GL         AFL +GV+ TL+ L K GHR+ L+  +P +   +      GP       TL RK+ +KLLQR+   Y+PPRV AWRYRRGQRSLL NL S                                  EAGGG               + GP    +                  E LEDIVE LL GLRDRDTVVRWSAAKG+GRI  RLP   AD+VV SVL  F E+  D AWHGGCLALAELARRG LLPERL  A P+V RAL YDVRRG   VGAHVRDAACYVCWAFARAYSP+ + P++  L   ML TALFDRE+N RRAAAAA QENVGRQG                 G   AHGI I+TAADYF+LGNRQ AYL IG  VA F  Y   I++ L L KL+H DP +R L + AL R+A L P++  + +LP  +    + D+  RHGA L +AE+ L L Q+   LP ET+  +V IVP +E+ARLYRGRGGEL+R AACRL+ECLAL    +++KTQLRLLDS+DE+LRH  ++V+  A AA++A    +F  G D PS R++ R +  Y   + T+EN A+ RG+  ALG LP +L       L     AL D    +  P R                           G EPDAETRRN + +L  + E +GV                           VSGG      R+G  G          +  A V  +++ LL  ++DY++DKRGDVGSW R+ AL G+E+ + L  R   GL                 PL          G   V+             PP Q     F       + P       S+ D +  A  D   A A+ T    D EV         ++P +   ++ AVLKQL EKLD+VRE AG VL  +       LP    R    ++    A    A G    +G ++      A  +P VV LL V  YH+AIV GLVISVGGL+ESVV  S   ++ W R                ACK    +   +   +  L+G+F   +GN R+++PLL++L+L                     D   A +   +  L   ++ E+ GCR+V K+     V + +L+          +     ALR  +L LGHRFP VRK  A+ +Y  LL ++ ++       E  L+ L   AW+  DLQ     R  +  +L +    A    GAG    +  GS   G     +E+ SY +LV+ +GY
Sbjct:   16 EEETSCESTYFVERDEVLARLTLLAGPEPP-ASLTGELEWLQKVLDKYIEQSTLLDPAMEKMLALTMGRARLIILSWHNKRLAEQVTA----PAPSFPFQIFRNAQLDALFAFVYVLCRLRGYKTVITFFPHEATDLEPVLHALQSQDRQDCGLWQTRYILLLWLSMLVLVPFNICSIDSGV--------GAGQSGRP----------QSSLTLVGAICDQATEYLRDPGPTREAAAVCLASLLKRPDMECGQLAVFLKWAEGVLGVRDPSAGAEMEERGQRGTGGGGLEATLAAPGEAFLPLGVLQTLSVLLKAGHRQNLLAFLPSLLRCLAQ----GPSSDLGSLTLSRKLKIKLLQRIALAYLPPRVAAWRYRRGQRSLLDNLRSVQ--------------------------------EAGGGR--------------RLGPERGTEEGGXXXXXXXXXXXXXXXEALEDIVEELLLGLRDRDTVVRWSAAKGLGRIASRLPESYADEVVASVLSSFTESAPDSAWHGGCLALAELARRGSLLPERLGLAVPVVTRALRYDVRRGQASVGAHVRDAACYVCWAFARAYSPQAMRPYVQRLATGMLLTALFDREINCRRAAAAAFQENVGRQG-----------------GRNFAHGIAILTAADYFTLGNRQAAYLSIGPAVAHFPPYTRSIIEHLALVKLRHPDPSIRLLGSQALHRMAGLAPSFLVDDILPTLVAQVDALDIYCRHGALLGVAELLLGLSQLPSFLPGETLKAVVAIVPRIEQARLYRGRGGELVRAAACRLIECLALALVPLSVKTQLRLLDSLDENLRHPTDAVRAHAVAAIRAFTHAYFGRGMDEPSARVQARVVDKYRRLVETDENVALTRGFLRALGALPSRL-------LFPSFPALLDTIETQLAPDRR-------------------------VGDEPDAETRRNGILALGEMAETLGVE--------------------------VSGGE-----RKGARG----------MNRAQVHRLISLLLKGTDDYAMDKRGDVGSWVRMAALQGLEKTVTLVRRGGGGLTGGAVEDSREGWQDGQRPLRVGDWVLTTHGRGQVRDLRCNEHLCEIAFPPGQAGAYLFPYGPGCGLFPRQSCQPLSVSDEVKDARTDDAAATAVGTASIGDAEVARFASSTSLISPELTTAIVSAVLKQLCEKLDAVRECAGRVLSTI-------LPLA--RDAEAYSTLLAAFLSEAEGGFSLKGGDS------AAVYPRVVRLLGVRAYHEAIVAGLVISVGGLTESVVKASVAAVVEWARQQRLSKRERQEECRSNACKDGHKMPPRLQEFSESLLGLFDAHRGNDRVIMPLLRSLDLFYQKGVLVPRAQAAGWEVGSEDGKHAGKASLSWRLADRLLREVTGCRNVTKLLACLRVALHLLSPLTFRAGQEEEEAGAAALRLVLLLLGHRFPRVRKLAAQELYTALLLHDHLLAGGGDAQERVLEALLGAAWEEEDLQALCGVRDEICAVLVLAPLGAGGTLGAGGTVVSRRGSDEGGREGAGEELASYAALVKEMGY 1428          

BLAST of mRNA_E-siliculosus-1a_F_contig122.1389.1 vs. uniprot
Match: A0A2R5GKZ4_9STRA (Tubulin-specific chaperone D n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GKZ4_9STRA)

HSP 1 Score: 793 bits (2047), Expect = 1.880e-255
Identity = 582/1596 (36.47%), Postives = 772/1596 (48.37%), Query Frame = 2

Query:  140 LAGREGILKAVKGALEKYLEQPHLLDPHLEDMMAVVMGKAKELVVEREEQVLAESGGGSRGRPG--EAFPFQVFICPQLHSMFCVIYQLCRVRGAKAVVRLMPHEAADLEPVIHALQAQDTDDYSTWETRYGLLLWLSMLSLVPFDIDTIDSGLRDSVPPGAGANEDGTKGVVAPVAGVGERQADLVGTILALGMRHLGDAGPTRDAAAACLSSLLTRPDMEAAHLRRFLEWSASVLERVTAEGRAGEEGLSKLAFLVMGVMATLAALFKQGHREKLIDLIPVVFYPVVGLAEL-GPGQTLLRKMLVKLLQRVGTTYVPPRVVAWRYRRGQRSLLQNLESTPDAASDGGDCCQDEQQAPSVEEKPLDRQEGETVEAGGGEGDAEESSAGGVGVAQDGPPAQVKVDATEVDVIAEEEEVDVPEELEDIVEALLCGLRDRDTVVRWSAAKGIGRITERLPRELADDVVCSVLDLFVEAEWDGAWHGGCLALAELARRGLLLPERLPRACPLVRRALAYDVRRGAGGVGAHVRDAACYVCWAFARAYSPRVLGPHLPGLCDSMLSTALFDREVNVRRAAAAALQENVGRQGQQHHSGEDDGSGWTDEGGGGVAHGIYIITAADYFSLGNRQQAYLDIGKTVAAFDRYRHHIVDTLRLDKLKHWDPELRRLSALALGRIASLEPAYAAETVLPAALGDTLSPDLLRRHGACLALAEVTLSLGQVSYALPAETVAGLVGIVPALEKARLYRGRGGELMRQAACRLVECLALTKSEVAIKTQLRLLDSVDESLRHAVESVQLSAAAALKAVLENWFPVGRDGPSER------------------------------------LRTRTIGLYVGGLRTEE-NAAVARGYAMALGVLPRKLAGASPAVLGEVLDALKDVALGRHGPSRSPSPAATGGGXXXXXXXXXXXXXIGGPGGEPDAETRRNAVRSLVTLCEEVGVGRQQQXXXXXXXSRDPGGGEGRQGSGGVSGGRDGVDAREGESGVPAAAACPAWLTLADVEGVLATLLGSSEDYSVDKRGDVGSWCRVEALAGME-------------------------------------RLLRLAARASRGLPLANRRGNASVQPPAQ-----QAPETFILPSLEDRLALL--------------DQPPAAMPTPHPCD-EVYLTPSMVERVMCAVLKQLSEKLDSVRERAGTVLQKLVQSDDPQLPFVPERAVVKHAITHGAATPAAPGAAGEEGKEAVNWAVPATTFPMVVGLLAVAEYHDAIVEGLVISVGGLSESVVTHSSKTLLRWLRACKSARNLRAVAGLALRLVGMFGDAKGNPRIVVPLLKTLELLLSNECFDSLPAEEHPFAELLLVA---VVAEMKGCRDVRKICLGASVFVCMLNYSDPVRPKALRNSVLFLGHRFPNVRKTVAE--------------------------------------------------AMYLKLLANE----------DVVDEAVYEEALDILTCTAWDG-DLQTAKAARSALAQLLGVNVPTANKKAGAGTGDAAGEGSGNPRKADEMESYESLVRT--VGY 4438
            LA  E  L  V+  LEKY EQ HLLD HLED++  +M   K ++  RE     E    SRG      +FPFQV+    L  +   +Y LC+VRG KAVV+ +PHE ADLEP +  LQ QD  DY+TWETRY LLLWLSML L+PFD++T+DS L       AG                G  Q+ LV T+  L    L D+G TR+AAA CL+ LLTRPDME+ HL  FL W+A  LE V+ + R G E  S+  FL+ G+   L  +FK GHREKL+  IPV+F  V+ ++   G   +L RK+ VKL+QR G  ++PPRVV WRY+RGQRSLLQNL  +  AA                                                                         D+PEE+ED+VE LL GLRDRDT+VRWSAAKGIGR+T RLP   ADDVV  VL+LF  +E D AWHGGCLALAELARRGLLLP+RL    P V +AL YDVRRG   VGA+VRDAACYVCWAFARAY P V+ PH+  LC  M+ TA+FDREVN RRAA+AA QENVGRQG  +                   HGI I+TAADYF+LG R+ AYL+I   VA FD YR  +++ L   KL HWD +LR LSA AL  + + +P + AETVL   L  TLSPD+L RHGA LA++ +  +L +V Y L  E  A +   V  +EK R YRGRGGE +R AAC+L+E +A    +++ + Q+RLL+SV+ESL+H  + V+ +A AAL A+ + +F  G D  +E                                     +  R +  Y   L T + N AV RG A+ALGVLP ++ GA  AVL  V+  L         P                                 DAETRRNAV SL  L   VGV                           V+G    +              C A L       V+  LLG+ EDY  D RGDVGSW RV A+ G+                                      R+LR  A  +            +V+ PA      ++ E F +PS ++R A+                +P A +   +P      L      R++CA++KQL EK+  VRE AG  L ++++  D     +P    V H                +   E  NW V + TFP V  +LA+++Y +A + GLV+SVG L+ESV ++++  LL W +A K+  N   +  +A  LV +  D     R+VVP LK L+LLL+ E F+      HP    LL     +  E+KG  +V K+   + V   + ++  P+RP AL + ++ LGH+FP VR + A+                                                   MY + LA+           D   E  +E+A D++  T WD   ++ A+  R  L +LL V  P   K A +   D+A      P   +E +SY +L+    +GY
Sbjct:   58 LAEAERALDGVQKILEKYQEQAHLLDRHLEDLVTRIMDAVKHVLRLREAD---EEARPSRGEDSAVNSFPFQVYRNAGLQLVLKALYLLCKVRGYKAVVKQLPHEVADLEPTLWLLQCQDAKDYTTWETRYSLLLWLSMLVLIPFDLETVDSSLA------AGTT--------------GASQSSLVATVTDLCKAFLNDSGATREAAAICLARLLTRPDMESVHLEGFLNWAADTLEDVS-DRREGGESRSEDPFLITGIFHALVEIFKHGHREKLLSHIPVIFSRVLRISNRDGQVSSLERKLTVKLVQRAGLNFLPPRVVKWRYQRGQRSLLQNLAKSAPAAD----------------------------------------------------AXXXXXXXXXXXXXXXXXXADIPEEVEDVVEKLLSGLRDRDTIVRWSAAKGIGRVTSRLPESHADDVVAFVLELFDASEGDAAWHGGCLALAELARRGLLLPKRLGAVVPFVVQALIYDVRRGHNSVGANVRDAACYVCWAFARAYEPAVMAPHVSELCPQMMVTAVFDREVNCRRAASAAFQENVGRQGHDN-----------------FPHGIEILTAADYFTLGIRKNAYLEIAPFVARFDVYRRPLIEHLVNYKLSHWDLQLRMLSADALHVLTASDPTFMAETVLRDLLPKTLSPDVLVRHGATLAVSTIVAALAKVPYRLSDEMRADVRNTVMRIEKGRWYRGRGGEWIRVAACKLIEAMARAGHQLSRRAQIRLLESVEESLKHPKDEVKEAAVAALAALADEYF--GPDANAEDGADALYEVANAADFSAAASRISRPLLPQMLPPVAADVTDRLVARYCQSLETPDPNPAVRRGCALALGVLPARMLGADRAVLERVVKCLAAATRLEDDPDLR------------------------------DAETRRNAVISLAKLASTVGVT--------------------------VAGAEKVL--------------CTATLDKELFTIVIDALLGACEDYETDNRGDVGSWVRVVAIKGLVTTIRAVRTFPQRALEAARAVQDDQAEIIASVGDMGRVRVLRYCADGAAAWVETLEPSGRAVKLPAGDLKTVESNERFSVPSEDERSAIFLPDAGARAIQSVMRQEPIADLGNYYPSAYSRLLDEPSTARIVCAIIKQLCEKMGQVRESAGEALVEILKDADSPAQGIP--LAVSHVDVLREIFDQ------KSSSEETNWKVESYTFPRVSQVLALSDYVNAALSGLVLSVGDLTESVSSNAADALLDWCKAQKARENYTQLTLIAKALVRIIEDNGRVDRVVVPALKALDLLLAAEVFNF---PRHPRLSWLLQTHDVIKQEIKGAANVSKLVNCSRVLFHLASFEGPIRPVALSSVLILLGHKFPLVRMSCAKDFNMWALAMDGTAICGPGNDAEAVLSAPVPEANPSNEGDSDFLGFLRQQNVMYEQELADRPDAFRPALSPDFDPEQAFEDAQDLIAETRWDTIPVRRAREQRDKLYELLRVQKPEGRKTAASAEVDSA---PAPPAPTEEEDSYATLIHNTQMGY 1474          

BLAST of mRNA_E-siliculosus-1a_F_contig122.1389.1 vs. uniprot
Match: A0A7S2WC38_9STRA (Hypothetical protein n=1 Tax=labyrinthulid quahog parasite QPX TaxID=96639 RepID=A0A7S2WC38_9STRA)

HSP 1 Score: 771 bits (1990), Expect = 2.290e-247
Identity = 533/1428 (37.32%), Postives = 731/1428 (51.19%), Query Frame = 2

Query:  182 LEKYLEQPHLLDPHLEDMMAVVMGKAKELVVEREEQVLAESGGGSRGRPGEAFPFQVFICPQLHSMFCVIYQLCRVRGAKAVVRLMPHEAADLEPVIHALQAQDTDDYSTWETRYGLLLWLSMLSLVPFDIDTIDSGLRDSVPPGAGANEDGTKGVVAPVAGVGERQADLVGTILALGMRHLGDAGPTRDAAAACLSSLLTRPDMEAAHLRRFLEWSASVLERVTAEGRAGEEGLSKLAFLVMGVMATLAALFKQGHREKLIDLIPVVFYPVVGLAEL-GPGQTLLRKMLVKLLQRVGTTYVPPRVVAWRYRRGQRSLLQNLESTPDAASDGGDCCQDEQQAPSVEEKPLDRQEGETVEAGGGEGDAEESSAGGVGVAQDGPPAQVKVDATEVDVIAEEEEVDVPEELEDIVEALLCGLRDRDTVVRWSAAKGIGRITERLPRELADDVVCSVLDLFVEAEWDGAWHGGCLALAELARRGLLLPERLPRACPLVRRALAYDVRRGAGGVGAHVRDAACYVCWAFARAYSPRVLGPHLPGLCDSMLSTALFDREVNVRRAAAAALQENVGRQGQQHHSGEDDGSGWTDEGGGGVAHGIYIITAADYFSLGNRQQAYLDIGKTVAAFDRYRHHIVDTLRLDKLKHWDPELRRLSALALGRIASLEPAYAAETVLPAALGDTLSPDLLRRHGACLALAEVTLSLGQVSYALPAETVAGLVGIVPALEKARLYRGRGGELMRQAACRLVECLALTKSEVAIKTQLRLLDSVDESLRHAVESVQLSAAAALKAVLENWF-------------------------------PVGRDGPSERLRTRTIGLYVGGLRTEE-NAAVARGYAMALGVLPRKLAGASPAVLGEVLDALKDVALGRHGPSRSPSPAATGGGXXXXXXXXXXXXXIGGPGGEPDAETRRNAVRSLVTLCEEVGVGRQQQXXXXXXXSRDPGGGEGRQGSGGVSGGRDGVDAREGESGVPAAAACPAWLTLADVEGVLATLLGSSEDYSVDKRGDVGSWCRVEALAGMERLLRLAAR---------------------------------ASRGLPLANRRGNASV---------QPPAQQAPETFILPSLEDRLALLDQPPAA-----MPTPHPCDEV----------YLTPSMVERVMCAVLKQLSEKLDSVRERAGTVLQKLVQ-SDDPQ--LPFVPERAVVKHAITHGAATPAAPGAAGEEGKEAV----NWAVPATTFPMVVGLLAVAEYHDAIVEGLVISVGGLSESVVTHSSKTLLRWLRACKSARNLRA-VAGLALRLVGMFGDAKGNPRIVVPLLKTLELLLSNECFDSLPAEEHPFAELLLVA---VVAEMKGCRDVRKICLGASVFVCMLNYSDPVRPKALRNSVLFLGHRFPNVRKTVAE 4162
            L+KY EQ HL+DP LE+++  +M  AK +V  RE+  L  SGG S      +FPFQ++    LH +F  +Y LC+VRG KAVV+ +PHE A+LEP +  LQ QD+ D+STWETRY LLLWLSML L+PFD+DT+DS          G N                 +  LVG++L L   +L D GP R AA+ CL+ L TRPDME+ +L  FL W+A  LE +    R  +E LS   FL+ GV  +L  +FK GHREKL+  IP++F  V+ +    G   +L RK+ +KL+QR G  ++PP+VV WRY+RGQRSLL                                                        G A+    AQV+++ +E      +E+VD+PEE+ED++E LLCGLRDRDT+VRWSAAKGIGRIT RLP ELADDVV  VL+LF  +E D AWHGGCLALAELARRGLLLP RL    P V +AL YDVRRG   VGA+VRDAACYVCWAFARAY P V+ PH+  L   ML TA+FDREVN RRAA+AA QENVGRQG  +                   +G+ I+TAADYF+LGNR  A+++I   V+ ++ YR  ++D L   KL HWD ELR LS+     +    P Y A+TVL   L  TLSPD+L RHGA L + E+T +L  + Y+L AE ++ +   +  +EKARLYRGRGGE MR AAC+L+  +A     ++ + QLRLLDS+DESL+H  + V+ +A  AL+A+   +F                               P       + +  R +  Y   L T + N AV RG A+ALG LP +   A+P  L  V+ AL        G S+                       +G      DAETRRNAV SL  + E VG+                                + VD       VP+     A L+L  +  +  TLL +  DY  D RGDVGSW R  A+  + +++R+  +                                 A   +     +G+ ++           PA+      +LP    R   + +  A+     + T  P +E+          YL P++   ++CA++KQL EK+ +VR+ AG  L +L++ +DD    +PF      V H  T       +  + GE   E      NW V + TFP V  LL+  EY DA V GLV+SVG L+ESV  +++  L  W +  KS  +  + +   A  L+ +  +     R+ VP +KT+ ++L  E    L  ++ P    LL     V AE+KGC +V K+ L   +F  +  ++ P R   L + ++ LGH +P VR   A+
Sbjct:   64 LDKYQEQSHLIDPFLEEIVGTLMESAKTVVQLREKDRLEASGGVS------SFPFQIYRNNGLHLVFTALYTLCKVRGYKAVVKQLPHEVAELEPTLLMLQCQDSKDHSTWETRYSLLLWLSMLVLIPFDLDTVDSN--------QGTNS----------------KLTLVGSVLELCKGYLSDPGPPRGAASLCLARLTTRPDMESRYLEEFLGWAAQTLEDIIKR-RTSDESLSTDTFLITGVFQSLVEIFKYGHREKLVSHIPIIFSRVLMITNRDGNISSLERKLTMKLVQRSGLNFLPPKVVKWRYQRGQRSLLH-------------------------------------------------------GAAKGVESAQVELEESE------DEDVDIPEEVEDVIEKLLCGLRDRDTIVRWSAAKGIGRITSRLPEELADDVVAYVLELFDVSEGDSAWHGGCLALAELARRGLLLPNRLDTVVPFVVKALVYDVRRGYNSVGANVRDAACYVCWAFARAYEPDVMAPHVGSLAPQMLVTAVFDREVNCRRAASAAFQENVGRQGHDNFQ-----------------YGVEILTAADYFTLGNRTNAFMEISAFVSKYEPYRKPLIDHLVSRKLVHWDLELRILSSKTFSVLTPKSPDYIAKTVLKDLLPMTLSPDMLIRHGATLGVGEITKALAAIPYSLDAEMLSDIRNTIMRIEKARLYRGRGGEWMRVAACQLIGAIAYAGHPLSARAQLRLLDSIDESLKHPKDEVKNAAVDALRALSTEYFGPNTKRDDGADVFSSNEDVEGLVLSHGRPLLPQSLQPVRQEILDRLVYKYCLALETNDPNPAVRRGCALALGALPARFLAATPNSLERVVKALV-------GASK-----------------------LGDDPDLCDAETRRNAVLSLGEVVESVGI--------------------------------EIVD-------VPSGYLLTAGLSLETIRLIFRTLLDACNDYEADNRGDVGSWVRCAAMKTLVKVVRIIRQFPSKTLEIVRSHDSKFKVVSTKSYGDVEVIRMTAGNSIAWVRSKGDNTLLKVSVENEFSEPAECDAAFGMLPDAAKRAEFVIKDVASKATSCIQTMTPLEELGKLTTPHYDQYLNPTVSTEIVCALIKQLCEKMGNVRDCAGDSLTELLEHADDVDYGIPFA-----VVHLDTIRTIFDVSKPSKGEYTDETFHFHCNWKVESDTFPRVSDLLSFGEYIDAAVGGLVLSVGDLTESVSKNAANALTAWCKEQKSCPSTTSKLTTFAHALLRILRENPKCDRVTVPTMKTIAMILEAEL---LSVKDQPRMAWLLDLHDLVKAEIKGCTNVTKLLLAGRIFFQLALFARPARTVVLGSILILLGHAYPKVRAGTAK 1305          

BLAST of mRNA_E-siliculosus-1a_F_contig122.1389.1 vs. uniprot
Match: B8BSV8_THAPS (Tubulin-specific chaperone d (Fragment) n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8BSV8_THAPS)

HSP 1 Score: 757 bits (1954), Expect = 6.870e-246
Identity = 509/1396 (36.46%), Postives = 719/1396 (51.50%), Query Frame = 2

Query:  161 LKAVKGALEKYLEQPHLLDPHLEDMMAVVMGKAKELVVEREEQVLAESGGGSRGRPGEAFPFQVFICPQLHSMFCVIYQLCRVRGAKAVVRLMPHEAADLEPVIHALQAQDTDDYSTWETRYGLLLWLSMLSLVPFDIDTIDSGLRDSVPPGAGANEDGTKGVVAPVAGVGERQADLVGTILALGMRHLGDAGPTRDAAAACLSSLLTRPDMEAAHLRRFLEWSASVLERVTAEGRAGEEGLSKLAFLVMGVMATLAALFKQGHREKLIDLIPV--VFYPVVGLAELGPGQTLLRKMLVKLLQRVGTTYVPPRVVAWRYRRGQRSLLQNLESTPDAASDGGDCCQDEQQAPSVEEKPLDRQEGETVEAGGGEGDAEESSAGGVGVAQDGPPAQVKVDATEVDVIAEEEEVDVPEELEDIVEALLCGLRDRDTVVRWSAAKGIGRITERLPRELADDVVCSVLDLFVEAEWDGAWHGGCLALAELARRGLLLPERLPRACPLVRRALAYDVRRGAGGVGAHVRDAACYVCWAFARAYSPRVLGPHLPGLCDSMLSTALFDREVNVRRAAAAALQENVGRQGQQHHSGEDDGSGWTDEGGGGVAHGIYIITAADYFSLGNRQQAYLDIGKTVAAFDRYRHHIVDTLRLDKLKHWDPELRRLSALALGRIASLEPAYAAETVLPAALGDTLSPDLLRRHGACLALAEVTLSLGQVSYALPAETVA-----GLVGIVPALEKARLYRGRGGELMRQAACRLVECLALTKSEVAIKTQLRLLDSVDESLRHAVESVQLSAAAALKAVLENWFPVGRDGPSERLRTRTIGLYVGGLRTEENAAVARGYAMALGVLPRKLAGASPAVLGEVLDALKDVALGRHGPSRSPSPAATGGGXXXXXXXXXXXXXIGGPGGEPDAETRRNAVRSLVTLCEEVGVGRQQQXXXXXXXSRDPGGGEGRQGSGGVSGGRDGVDAREGESGVPAAAACPAWLTLADVEGVLATLLGSSEDYSVDKRGDVGSWCRVEALAGMERLLRLAARASRGLPLANRRGNASVQPPAQQAPETFILPSLEDRLALLDQPPAAMPTPHPCDEVYLTPSMVERVMCAVLKQLSEKLDSVRERAGTVLQKLVQSDDPQLPFVPERAVVKHAITHGAATPAAPGAAGEEGKEAVNWAVPATTFPMVVGLLAVAEYHDAIVEGLVISVGGLSESVVTHSSKTLLRWLRACKSARNLRAVAGLALRLVGMFGDAKGNPRIVVPLLKTLELLLSNECFDSLPA-EEHPFAELLLVAVVAEMKGCRDVRKICLGASV-FVCMLNYSDPVRPKALRNSVLFLGHRFPNVRKTVAEAMYLKLLANEDVV--DEAVYEEALDILTCTAWDGDLQ---TAKAARSALAQLL 4306
            L  ++  L+KYLE P LLDP+LE M+                Q L    G +  +              L  +   IY L +VRG K + RL+PHEAAD+EPV+  LQA   ++   WE+ Y LL WL ++SLVPFD+  IDS L +            T    A  A        LV +IL     HL D GPTR+ AAACL+SLL+RPD+E + L  F+ WSA     +                LVMGV+ TLAA+FK GHR  L+       +               LLRK+LVKL  R+G  Y+PPRV AWRY+RG+RSL++NL    DA++             +   K ++ +EG                        DG   Q+                  P+++ED ++ LL  L D  T+VRWSAAKGIGR+TERLP   ADDV+ +VL    + E D AWHG CLALAELARRGLLLP+RL    P+V +++ YDVRRG   VGAHVRDAACY CWAFARAY+P VL PH+  L  +++ ++LFDREVN RRAA+AA QE+VGRQG  +                   HGI I+T+ADY+S+GNR +++L I   +A F  YR  I+  L   K+ HWD E+R L++ AL R++ L+P+Y A  V+P  +    S DL+ RHG+ L +AE+ L+ G+++      T++      +  +VP++EKARLYRGRGGE+MR AACR +EC++     + +K Q+RLLDSVD  L H  E++Q SAA AL A+L ++FPV   GPSERL++R +  Y+  +++E+N A  RG+++ALG LP KL   S  VL  VLD L       H   +S                          GGE DAETRRNA+ SL+ +C+  G+G                                        S + A  +    LT    E V A LL + EDY++D+RGDVGSW RV A+ G+E L                               T++  S +                      +    +   ++ A+LKQL EKLD+VR  AG  L++L+ +++P+LPFVP R ++  A+              +  K+  NW+ PA TFP+++  + +  + D I+ GLVISVGGL+ESV   SS +L  W+R  +SA+    +  +    +G+F   K N R+++PLL TL+ LLS+   D L + ++  F   L++ +  E KGC DV+++     V F  +  + + ++  AL   +  L + +P VR+ VAE ++ KL  + D +  D    EEA  +L    W  +     T   AR+ +A LL
Sbjct:    6 LSRLRSILDKYLECPTLLDPYLEGMV----------------QRLRNDDGSTTAK-----------LQTLMHLLSGIYALSKVRGRKYIQRLLPHEAADVEPVLAMLQAMG-EEAKVWESVYSLLTWLGIISLVPFDLHIIDSSLEE------------TNDAKATTA-------TLVQSILNTSTSHLDDPGPTRETAAACLASLLSRPDLEQSELEGFVNWSAQYTPTILPMPDKAPS-----VILVMGVLQTLAAIFKTGHRSNLLSTQQKQSILVAEXXXXXXXXXXXLLRKLLVKLFARIGCAYLPPRVAAWRYQRGKRSLVENL-MRGDAST----------AVVTESVKSVEVEEGR----------------------DDGVLFQI------------------PDQVEDAMDQLLRSLTDPATIVRWSAAKGIGRLTERLPAMCADDVLDAVLQTCSDHEHDRAWHGACLALAELARRGLLLPDRLGEVVPIVVQSIRYDVRRGQHSVGAHVRDAACYTCWAFARAYAPAVLRPHVKELSVALVLSSLFDREVNCRRAASAAFQESVGRQGADNFK-----------------HGIAILTSADYYSIGNRTESFLTISLDIAKFKEYREPIIQHLSRVKVVHWDAEIRTLASRALSRVSVLDPSYCATKVMPTLIQQCFSDDLIVRHGSLLGVAEMVLAFGELNLVQGDATMSDDLKLSIAELVPSIEKARLYRGRGGEIMRAAACRTIECISTANITLTVKQQVRLLDSVDACLVHPNETIQNSAAKALLALLTSYFPVSSKGPSERLQSRVVDKYISIVKSEDNPAATRGFSLALGHLPGKLLAPSEQVLDSVLDCLI------HASKKSTL-----------------------VGGEGDAETRRNAILSLINVCKTAGIG---------------------------------------HSSLEANLSPTNSLTTCQTERVFAALLSAMEDYNMDRRGDVGSWSRVAAMNGLEAL-------------------------------TYLSYSAK----------------------FFDEGLCCSILSALLKQLGEKLDAVRCEAGECLERLLTNNNPRLPFVPHRTMLIRAL--------------DLNKQGKNWSNPAMTFPLLMCAINIDVFLDPILSGLVISVGGLTESVSKSSSASLFEWIRDLRSAKATSKLYQMGEVFLGLFDKNKRNGRVLLPLLATLDKLLSHGYMDELLSRKDGAFLSCLMICLSNESKGCSDVKRLLAIVGVSFNVIQPHLETMKEMALPFIMTMLLNSYPRVRRYVAEQLFAKLSVDGDALFDDHECLEEANQLLLSVVWHDEHDPCGTITEARNRIADLL 1146          

BLAST of mRNA_E-siliculosus-1a_F_contig122.1389.1 vs. uniprot
Match: A0A8K1C6S2_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1C6S2_PYTOL)

HSP 1 Score: 756 bits (1952), Expect = 2.530e-242
Identity = 558/1581 (35.29%), Postives = 789/1581 (49.91%), Query Frame = 2

Query:   59 ATCERILFAEREQVMGMLQQLV-ATDLDLAGREGILKAVKGALEKYLEQPHLLDPHLEDMMAVVMGKAKELVVEREEQVLAESGGGSRGRPGEA-FPFQVFICPQLHSMFCVIYQLCRVRGAKAVVRLMPHEAADLEPVIHALQAQDTDDYSTWETRYGLLLWLSMLSLVPFDIDTIDSGLRDSVPPGAGANEDGTKGVVAPVAGVGERQADLVGTILALGMRHLGDAGPTRDAAAACLSSLLTRPDMEAAHLRRFLEWSASVLERVTAEGRAGEEGLSKLA--FLVMGVMATLAALFKQGHREKLIDLIPVVFYPVVGL----------AELGP--GQTLLRKMLVKLLQRVGTTYVPPRVVAWRYRRGQRSLLQNLESTPDAASDGGDCCQDEQQAPSVEEKPLDRQEGETVEAGGGEGDAEESSAGGVGVAQDGPPAQVKVDATEVDVIAEEEEV--DVPEELEDIVEALLCGLRDRDTVVRWSAAKGIGRITERLPRELADDVVCSVLDLFVEAEWDGAWHGGCLALAELARRGLLLPERLPRACPLVRRALAYDVRRGAGGVGAHVRDAACYVCWAFARAYSPRVLGPHLPG-LCDSMLSTALFDREVNVRRAAAAALQENVGRQGQQHHSGEDDGSGWTDEGGGGVAHGIYIITAADYFSLGNRQQAYLDIGKTVAAFDRYRHHIVDTLRLDKLKHWDPELRRLSALALGRIASLEPAYAAETVLPAALGDTL--SPDLLRRHGACLALAEVTLSLGQVSYALPAETVAGLVGIVPALEKARLYRGRGGELMRQAACRLVECLALTKSEVAIKTQLRLLDSVDESLRHAVESVQLSAAAALKAVLENWFP-VGRDGPS--ERLRTRTIGLY-------------VGGLRTEENAAVARGYAMALGVLPRKLAGASPAVLGEVLDALKDVALGRHGPSRSPSPAATGGGXXXXXXXXXXXXXIGGPGGEPDAETRRNAVRSLVTLCEEVGVGRQQQXXXXXXXSRDPGGGEGRQGSGGVSGGRDGVDAREGESGVPAAAACPAWLTLADV--EGVLATLLGS-SEDYSVDKRGDVGSWCRVEALAGMERLLR---LAARASR----GLPLANRRGNASVQPPA----QQAPETFILPSLEDRLALLDQPPAAMPTPHPC------------------------------------DEVYLTPSMVER-------------------------VMCAVLKQLSEKLDSVRERAGTVLQKLVQSDDPQLPFVPERAVVKHAITHGAATPAAPGAAGEEGKEAVNWAVPATTFPMVVGLLAVAEYHDAIVEGLVISVGGLSESVVTHSSKTLLRWLRACKSARNLRAVAGLALRLVGMFGDAKGNPRIVVPLLKTLELLLSNECFDSL-----PAEEHP----FAELLLVAVVAEMKGCRDVRKICLGASVFVCMLNYSDPVRPKALRNSVLFLGHRFPNVRKTVAEAMYLKLLANEDVVDEAVYEEALDILTCTAWDGDLQTAKAARSALAQLLGVNVPTANKKAGAGTGDAAGEGSGNPRKADEMESYESLVRTVGY 4438
            A  +R  F ER QV  +L+ +  A  +D    E   K +   L++YLEQ HLLDP+L +++  ++ + K +V     +  AE    + G  G A FPFQ+   P+LH +  +IY LC+VRG K +V+L+PHE +D EP +  LQ+QD  D+STWETRY LLLWLSML LVPFD++TIDS    +                       +    +V +IL L   +L D G T+ AAA CL+ LL+RPDMEA +L RFL W+   L  VTA   A +  L      F + G+M +LA L K   REK ID   + F  V+ L           E  P    TL RK+ VKL+QR+G  Y+PPRV AWRY+RG RSL  N+++   A++  G         PS                GGG+                             D   +++++  D  EELE IVE LLCGLRD+DTVVRWSAAKGIGRIT RLP E ADDVV SVL+LFV  E +GAWHG  LALAELARRG+LLP+RL  A   V RAL YD+RRGA  +G+HVRDAACY CW+FARAY P +L PHL   L  +ML   +FDRE+N RRAA+AA QENVGRQG+ +                   HGI ++T ADYFS+ N + A+LD+   +A +  YR+ +V+ L + K+ HWD  +R L+A ALG+I +L+ A+A   VLP  L   L    +++ RHGA LA+ EVTL+L  +   +  E  + +  +   +EK RL+RGRGGE++R A C++V+ +A+    ++     + +  ++E + H VESV+ +A  A  A    + P +   GP    +  T  +  Y             V  +    N AV RG+  A+GV  ++L   +   L E L  +   A+ +H  +                              E DAE+R  A+R L  LC  V                                  DG                   L   DV  E ++ TL+    +DY +D+RGDVGSW R EA+ G+ERL      A  AS     GL + +R G+ ++        ++  +     S  D+ AL   P   +    P                                     DE +L P+  ER                         V+CA+ KQL+EKLD++R  AG++L +L+ S +P++  +P+R  ++  I                   A+NW++   TFP+VV ++ + EY +A+  GLV+SVGGL+ESVV  S   L  W R   +A++   ++  +  LV +    + + R+ +PLLKTL +LL +  F  L       EE+     F + L  A+  EM+    + K+  G SV V +L        KALR  ++F+ HRFP VRK  AE +Y +LL ++DVV E  Y+   DIL+CTAWDG +   +AAR+ + + LG++VP   KKA     D A   +   R A    SY++LV+ +GY
Sbjct:   21 ACSQRKFFQERAQVQELLKDVCNAVTVDDEQFERSHKQITTILDRYLEQSHLLDPYLRELLDPILSEIKRIVATVASERAAERQ--TAGDDGVAVFPFQLRRDPKLHRLCQLIYHLCKVRGYKTIVKLLPHEVSDFEPTLMMLQSQDRSDHSTWETRYVLLLWLSMLCLVPFDLNTIDSSTSTTT----------------------DTNLSIVSSILTLCKGYLADPGATQLAAAVCLARLLSRPDMEAHYLERFLAWANQELASVTASTGAPQSHLDGRVQQFRITGIMLSLAYLAKHSPREKHIDATRIYFASVMQLIEKLTDDDSHGEYRPIHSSTLHRKLSVKLVQRLGLLYLPPRVQAWRYKRGLRSLELNMQAIGLASATKG---------PST--------------LGGGQ-----------------------------DTTGQDDDIMFDAVEELEQIVEVLLCGLRDKDTVVRWSAAKGIGRITGRLPYEYADDVVQSVLELFVSTESEGAWHGASLALAELARRGVLLPQRLTEAVDCVARALQYDIRRGAHSIGSHVRDAACYACWSFARAYEPLLLLPHLEKTLAPAMLINCVFDRELNCRRAASAAFQENVGRQGRTN-----------------FPHGIDLLTKADYFSVANLRHAFLDVSVFIAKYPEYRYSLVEHLVMQKITHWDASIRSLAAQALGKICALDAAHAMHFVLPRLLETALVAESEVIARHGAVLAICEVTLALLDLPSLIDGELQSKIKNLAIEMEKRRLFRGRGGEMIRVAVCQVVQVVAIGGFSLSFAHVKKYVSVLEECIIHPVESVRDAAIDAFAAFTAQYCPKMFERGPPPVRQFLTELVPRYLNNGVLVTMKERGVSSIVLNPNVAVRRGFLRAIGVASKELIQPT---LSESLRVMMRAAVMKHQTAE-----------------------------EQDAESRVAAIRGLADLCSRVQC--------------------------------DG-------------------LVFGDVAEEEIVRTLIECVQDDYGMDERGDVGSWVRKEAMLGLERLYLNEPFAGCASELLLVGLRVRSRYGDGTIVKVTGRCRRRQSDAGDTTSETDKGALALNPICHVQFTKPALGFYYFAPLGIGQIHWSELHANEKELSRRRNGGDGTDEPFLIPAHSERLLTPARAEPPSLPFIRRVPVTITSEVVCALTKQLAEKLDNIRLLAGSILFRLLHSTNPRVDGIPDRFQLEKTIFPPTL--------------AINWSMAHDTFPLVVRMMDIPEYMEAVASGLVVSVGGLTESVVKASKAALFEWFRTHLNAKDFGLLSRFSFFLVTLIRRHQQDDRVTIPLLKTLAMLLDDGLFAFLFEKKSSDEENEAAASFGDRLYDALKDEMQKATAMPKLAAGISVLVGLLPSDTETEGKALRALLMFIAHRFPKVRKLTAEKLYTRLLVHDDVVPEDKYDAITDILSCTAWDGAVTEVRAARNEVLEHLGMDVP--QKKARVDKIDEAASTATETRSASS--SYQALVKEMGY 1407          

BLAST of mRNA_E-siliculosus-1a_F_contig122.1389.1 vs. uniprot
Match: A0A7S3Q9K5_9STRA (Hypothetical protein n=1 Tax=Chaetoceros debilis TaxID=122233 RepID=A0A7S3Q9K5_9STRA)

HSP 1 Score: 746 bits (1925), Expect = 6.480e-238
Identity = 526/1530 (34.38%), Postives = 794/1530 (51.90%), Query Frame = 2

Query:  152 EGILKAVKGALEKYLEQPHLLDPHLEDMMAVVMGKAKELVVEREEQVLAESGGGSRGRPGEAFPF-----------------------QVFICPQLHSM---FCVIYQLCRVRGAKAVVRLMPHEAADLEPVIHALQAQDT-DDYS---------------TWETRYGLLLWLSMLSLVPFDIDTIDSGLRDSVPPGAGANEDGTKGVVAPVAGVGERQADLVGTILALGMRHLGDAGPTRDAAAACLSSLLTRPDMEAAHLRRFLEWSASVLERVTAEGRAGEEGLSKLAFLVMGVMATLAALFKQGHREKLID----LIPVVFYPVVGLAELGPGQT--------------------LLRKMLVKLLQRVGTTYVPPRVVAWRYRRGQRSLLQNLESTPDAASDGGDCCQDEQQAPSVEEKPLDRQEGETVEAGGGE---GDAEESSAGGVGVAQDGPPAQVKVDATEVDVIAEEEEVDVPEELEDIVEALLCGLRDRDTVVRWSAAKGIGRITERLPRELADDVVCSVLDLFVEAEWDGAWHGGCLALAELARRGLLLPERLPRACPLVRRALAYDVRRGAGGVGAHVRDAACYVCWAFARAYSPRVLGPHLPGLCDSMLSTALFDREVNVRRAAAAALQENVGRQGQQHHSGEDDGSGWTDEGGGGVAHGIYIITAADYFSLGNRQQAYLDIGKTVAAFDRYRHHIVDTLRLDKLKHWDPELRRLSALALGRIASLEPAYAAETVLPAALGDTLSPDLLRRHGACLALAEVTLSLG--------QVSYALPAETVAGLVGIVPALEKARLYRGRGGELMRQAACRLVECLALTKSEVAIKTQLRLLDSVDESLRHAVESVQLSAAAALKAVLENWFPVGRDGPSERLRTRTIGLYVGGLRTEENAAVARGYAMALGVLPRKLAGASPAVLGEVLDALKDVALGRHGPSRSPSPAATGGGXXXXXXXXXXXXXIGGPGGEPDAETRRNAVRSLVTLCEEVGVGRQQQXXXXXXXSRDPGGGEGRQGSGGVSGGRDGVDAREGESGVPAAAACPAWLTLA----DVEGVLATLLGSSEDYSVDKRGDVGSWCRVEALAGMERLLRLAARASRGLPLANRRGNASVQPPAQQ-APETFILPSLEDRLALLDQPPAAMPTP------------HPCDEVYLTPSMVERVMCAVLKQLSEKLDSVRERAGTVLQKLVQSDDPQL-PFVPERAVVKHAITHGAATPAAPGAAGEEGKEAVNWAVPATTFPMVVGLLAVAEYHDAIVEGLVISVGGLSESVVTHSSKTLLRWLRACKSARNLRAVAGLALRLVGMFGDAKGNPRIVVPLLKTLELLLSNECFDS-LPAEEHPFAELLLVAVVAEMKGCRDVRKICLGASVFVCMLNYS-DPVRPKALRNSVLFLGHRFPNVRKTVAEAMYLKLLANEDVVDEAVYEEA-LDILTCTAWDGDLQ---TAKAARSALAQLLGVNVPTANKKAGAGTGDAAGEGSGNPRKA-DEMESYESLVRTVG 4435
            +  L  ++   +KYLE   L+DPHLE +++ +   AK ++ +       E                                      +  I   + ++      IY + +VRG K + + +PH+AAD+EPV+  L+  +   DY+                WE+ Y LLLWL MLSLVPFD++TIDS L  +       +                 +  L+ ++LA   +HL DAG TR+AAA+ L+SLL+RPD+E A L  F+ +S  VL+           G     FLVMGV+ TLA +FK G R  L++     + +++   + +AE    Q                     +LRK+LVKL  RVG +Y+PP++ +WRY+RG+RSLL+NLE+T +                          E ET  A GG+   G+  +++AG    +QD                 + +  DVP+++ED +  L+  L D  T VRWSAAKGIGR++ERLP   ADD++ ++L+L  + E D AWHG CL+LAELARRGLLLP+RL    P+  RA+ YD+ RG   VG+HVRDAACY CWAFARAY+P VL P++P L  +++  +LFDRE+N RRAA+A+ QE VGRQG  +                   HGI I+TAADYFSLGN+  AY  + + +A +  YR  I+  L  +KL HWDPE+R LS+++L  +A++EPA+ A+TVLP  +G  +  +L  RHGA + +AE+ L+LG             +  E +A +  +V A+EKARLYRGRGGE+MR A  RL+EC+AL +  + +K Q+ LLDS+D +L+H  E +Q++A++AL +++ ++FPV   GPSERL++R +  YV  +R +EN A  RGY++ALG LP KL   +P V G  LDA+ D       PS                        IGG G   DAETRRN+++SLV +C+ VGVG                          +S                 A A P++ T+A     V  V   L  + EDY+ D+RGDVGSW R+EA+ GME L  L  +AS      N     S  P   +   +   +PS+  RL  L+   +                 +   + Y    +V +V+ ++LKQLSEKLD+VR +AG+ L++++      + PFVP +A++  A+               +     NW+ PA TFP+++  + +  + ++I+ G++ISVGGL+ES+   SSK L+ ++RA    + +  +A +   L+ +F     + R+++PLL T++ LLS+   D+ L +  + F++ L + V  E   C D++++     V + +L+      +   L   +  L H+FP VR+  AE +Y+KL+ +E VV  A   EA  D+L+  +WD +L      +A R+ +A  LG+ +   ++K      D +G       K  D+ ESY SLV T G
Sbjct:   82 DAALARLRVIFDKYLECSTLMDPHLERIVSSLSSSAKHIIHDIYNITTTEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTVETIIDANIQNLKRHLSAIYAISKVRGRKHIKKFLPHDAADVEPVLVTLRLMEHYKDYNQNYNSCGFGSEIQAYAWESMYTLLLWLGMLSLVPFDLNTIDSSLTLN-------HGXXXXXXXXXXXXXXXXKMTLISSMLATAQKHLSDAGATREAAASSLASLLSRPDLEQAELEDFVMFSNDVLKGYLRGDDGTGNGKDVSVFLVMGVIQTLATIFKTGSRSNLMERHLRCVEMLWEQAILVAEKAQPQQSSDGFGSSDXXXXXXXXXVLVLRKLLVKLFARVGCSYLPPKIASWRYQRGRRSLLENLENTSN--------------------------ENETGNADGGKSGNGNGTDNAAGLRDRSQD-----------------DSDLFDVPDQVEDSMAQLIQALTDPATTVRWSAAKGIGRVSERLPAICADDILDAILELCNDEENDNAWHGACLSLAELARRGLLLPKRLGEVIPIAIRAIQYDIPRGQHSVGSHVRDAACYTCWAFARAYAPNVLKPYVPELSKAIVVASLFDREINCRRAASASFQECVGRQGADNFK-----------------HGIAILTAADYFSLGNKTDAYTTVARQIAQYQEYRQAIISHLYEEKLFHWDPEIRDLSSVSLRGLATMEPAHFAKTVLPCLVGYAMHENLFVRHGAVIGVAEIVLALGGDNEEGIENTGSGISEELIASISDLVFAIEKARLYRGRGGEIMRAAVSRLIECMALAQVPMNVKQQIGLLDSLDANLKHPNEIIQVAASSALYSLMRSYFPVRESGPSERLQSRVVDKYVESVRGDENPAATRGYSLALGYLPAKLL--APTVEG--LDAVIDCLCVSSHPST----------------------LIGGQG---DAETRRNSIKSLVRICQVVGVG--------------------------IS-----------------ADANPSYPTVAMDGSQVMRVFKALFDAVEDYNTDRRGDVGSWSRIEAMKGMEALTYLVIKAS------NIPHTMSCGPEENENTKDVPCVPSIARRLRYLEADVSTRVRSCLSESKPFRQFSYSSTQTYFDDELVSKVLSSILKQLSEKLDAVRGQAGSCLERMLSEKTSIIVPFVPSKALLLEALRLN------------DIPLQHNWSNPAVTFPLMMKAINIEAFFESILAGMIISVGGLTESITKSSSKALMDYMRALHKIKAVGKIAKIGHGLIKLFDKHTKDGRVILPLLVTVDKLLSHGLLDAILVSPTNDFSKDLSLRVRREASRCNDIKRLMAIVPVALSILHCEVAETQNTTLLFLMRLLAHKFPRVRRHTAEQLYIKLVEDESVVPNATNIEAGNDLLSQASWDRELGPPGNVRAYRNQVANFLGIEL---SEK------DLSGPVMKKVVKVKDDFESYASLVSTAG 1445          

BLAST of mRNA_E-siliculosus-1a_F_contig122.1389.1 vs. uniprot
Match: A0A5D6XM80_9STRA (TFCD_C domain-containing protein n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XM80_9STRA)

HSP 1 Score: 738 bits (1905), Expect = 8.190e-236
Identity = 565/1545 (36.57%), Postives = 744/1545 (48.16%), Query Frame = 2

Query:   35 VGSMGDNHATCERILFAEREQVMGMLQQLVATDLDL----AGREGILKAVKGALEKYLEQPHLLDPHLEDMMAVVMGKAKELVVEREEQVLAESGGGSR--GRPGEAFPFQVFICPQLHSMFCVIYQLCRVRGAKAVVRLMPHEAADLEPVIHALQAQDTDDYSTWETRYGLLLWLSMLSLVPFDIDTIDSGLRDSVPPGAGANEDGTKGVVAPVAGVGERQADLVGTILALGMRHLGDAGPTRDAAAACLSSLLTRPDMEAAHLRRFLEWSASVLERVTAEGRAGEEGLSKLAFLVMGVMATLAALFKQGHREKLIDLIPVVFYPVVGL----------AELGPGQTLLRKMLVKLLQRVGTTYVPPRVVAWRYRRGQRSLLQNLESTPDAASDGGDCCQDEQQAPSVEEKPLDRQEGETVEAGGGEGDAEESSAGGVGVAQDGPPAQVKVDATEVDVIAEEEEVDVPEELEDIVEALLCGLRDRDTVVRWSAAKGIGRITERLPRELADDVVCSVLDLFVEAEWDGAWHGGCLALAELARRGLLLPERLPRACPLVRRALAYDVRRGAGGVGAHVRDAACYVCWAFARAYSPRVLGPHLPG-LCDSMLSTALFDREVNVRRAAAAALQENVGRQGQQHHSGEDDGSGWTDEGGGGVAHGIYIITAADYFSLGNRQQAYLDIGKTVAAFDRYRHHIVDTLRLDKLKHWDPELRRLSALALGRIASLEPAYAAETVLPAALGDTLSPDL--LRRHGACLALAEVTLSLGQVSYALPAETVAGLVGIVPALEKARLYRGRGGELMRQAACRLVE---CLALTKSEVAIKTQLRLLDSVDESLRHAVESVQLSAAAALKAVLENWFP-VGRDGPSER------LRTRTIGLYVGGLRTEE---------NAAVARGYAMALGVLPRKLAGASPAVLGEVLDALKDVALGRHGPSRSPSPAATGGGXXXXXXXXXXXXXIGGPGGEPDAETRRNAVRSLVTLCEEVGVGRQQQXXXXXXXSRDPGGGEGRQGSGGVSGGRDGVDAREGESGVPAAAACPAWLTLADVEG-VLATLLGS-SEDYSVDKRGDVGSWCRVEALAGMERLL-------RLAARASRGLPLANRRG-------NASVQPPAQQAPET--------------------------------------------FILPSLEDRLALLDQP----------PAAMPTPHPCDEVYLTPSMVERVMCAVLKQLSEKLDSVRERAGTVLQKLVQSDDPQLPFVPERAVVKHAITHGAATPAAPGAAGEEGKEAVNWAVPATTFPMVVGLLAVAEYHDAIVEGLVISVGGLSESVVTHSSKTLLRWLRACKSARNLRAVAGLALRLVGMFGDAKGNPRIVVPLLKTLELLLSNECFDSLPAE-EHPFAEL-------LLVAVVAEMKGCRDVRKICLGASVFVCMLNYSDPVRPKALRNSVLFLGHRFPNVRKTVAEAMYLKLLANEDVVDEAVYEEALDILTCTAWDGDLQTAKAARSALAQLLGVNVP 4321
            V   GD   + E+  F E  QV  +L +L+A   D+    A  E     +   L++YLEQ HLLDP L ++   +M   K  V        A +G           AFPFQV+  P+LH +  ++Y LC+VRG K VV+L+PHEA+D EP +  LQ+QD  D+STWETRY LLLWLSML LVPFD++TIDS +     PGAGA   G   +V                 L L   +LGD G T+ AA+ CLS LL+RPDMEA +L RFL W+ S L    A    G +  +   F V GVM +LA + K   RE+      + F  V+ L          A+     TL RK+ VKL+QR+G  Y+PPRV +WRY+RG RSL  N++S                                 + AG GEG    S        +  P   V ++A E              ELE IVE LLCGLRD+DTVVRWSAAKG+GRIT RLP E ADDVV SVL+LFV  E DGAWHG  LALAELARRG+LLPERLP A   V RAL YDVRRG   +G+HVRDAACY CW+FARAY   +  P L   L  +ML   +FDRE+N RRAA+AA QE VGRQG+ +                   HGI ++T ADYFS+ N + AYLD+   VA+F  YR+ +++ L   KL HWD ++R L+A ALGRIA+L+P +A   + P  L   L+ D+  + RHGA LALAE+ L+L +V   L  E    L  +   ++K RL+RGRGGEL+R A C +VE   C AL  S   +K    +L+   E   H VESV+ +A  A  A    + P +   G +        L  R +   V    TE          N A  RG+  A+GV    L   S   +G  L AL   A  R   +                              E DAE+R  AVR+LV +C                 SR                                       L LA +E  V+ TL+    EDY VD+RGDVGSW R EA+ G+ERLL         A +A  G  +A+  G        A ++  A   P T                                            F LP   DRL    QP          P A     P  +    P +V    C + KQL+EKLD VR  AG  L +++ S  P++  +P+R  ++  +   +                VNW++   TFP+ V +++  EY +A+  GLV+SVGGL+ESVV  S   L  W+R     +N+  +   A  LV +        R+ +PL+KTL +LL       L A  +H  A+        L  AV  E++    V KI  G +V   +L        KAL+  VLFLGHRFP VRK  AE +Y++LL +E+VVDEA Y+  ++IL+ TAWD  +   + AR+ L  LL +  P
Sbjct:   16 VPEAGDESCS-EKKFFEEHAQVSQLLAELLAAPPDVTEVDAAFERQCATIATILDRYLEQAHLLDPFLRELTEPIMAHVKRAV--------AATGAACEPPAAANVAFPFQVYRNPRLHKLLQLVYHLCKVRGYKTVVKLLPHEASDFEPTLRLLQSQDRSDHSTWETRYVLLLWLSMLCLVPFDLNTIDSSI-----PGAGAAAGGAIAIVTDT--------------LTLCKEYLGDPGATQLAASVCLSRLLSRPDMEALYLHRFLAWANSEL---AAAAHQGADLRAANQFRVTGVMRSLAHIAKNAPREQHAQASRIYFTSVLQLIARMADEDARADRPSSSTLHRKLSVKLVQRLGLLYLPPRVQSWRYQRGLRSLELNMQSIG------------------------------LLPAGTGEGGVATSXXXXXXXXEHEPDDDVGLEAVE--------------ELEQIVEVLLCGLRDKDTVVRWSAAKGVGRITGRLPYEFADDVVQSVLELFVATEGDGAWHGASLALAELARRGVLLPERLPEAADCVARALRYDVRRGTHSIGSHVRDAACYACWSFARAYDAALFLPRLERTLAPAMLINCVFDRELNCRRAASAAFQECVGRQGRAN-----------------FPHGIELLTRADYFSVSNLRHAYLDVSVFVASFPEYRYCLLEHLLATKLSHWDVQVRTLAAAALGRIATLDPGFAVAQLFPRLLASALAGDVEVIVRHGATLALAELLLALARVPVFLDGEVQKQLKMLPIEVDKRRLFRGRGGELIRAAVCNVVEAIACAALALSFAHVKKYFAVLE---ECFVHPVESVRTAAINAFAAFTAQYCPKIFEKGSAAHVAFLRALVPRYLNAGVLMTTTERGASTVALNANVAARRGFIRAIGVSADALLQPS---VGACLTALMRAASIRRQSAD-----------------------------EQDAESRVAAVRALVDICSRA-------------RSR---------------------------------------LDLAHLEDDVVRTLVQCIREDYGVDERGDVGSWVRKEAIVGLERLLLGESSYDASALQALVGDRVASAYGPGTITRVQAGLRDAADHEPSTSDPICAVQFEKPAFGFYYFPPHGVGLLRLSKLRVSDEENAIDAAFQLPGFADRLEQAQQPLERSSERVGSPQAARQQLPFAKRVSAP-LVGEFFCELGKQLAEKLDGVRLVAGRTLFRVLHSTSPRVDAIPDRLALEQTVFPPSLQ--------------VNWSMAHDTFPLTVQMMSSPEYLEAVASGLVLSVGGLTESVVKSSKAALFAWVRRHTEQKNVGLLTRFAFFLVALLTHHSQEDRVTLPLMKTLAILLEANVLGFLFATAKHDTADARAGFGAQLYDAVRDEIQRTTSVPKIAAGVAVLTGLLPSEPATERKALKALVLFLGHRFPKVRKVAAEKLYMRLLVHEEVVDEAKYDAVVEILSETAWDASIAHVREARNELLDLLDMERP 1366