mRNA_E-siliculosus-1a_F_contig1191.1225.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig1191.1225.1
Unique NamemRNA_E-siliculosus-1a_F_contig1191.1225.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig1191.1225.1 vs. uniprot
Match: D7G288_ECTSI (D-lactate dehydrogenase, putative n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G288_ECTSI)

HSP 1 Score: 139 bits (351), Expect = 3.810e-38
Identity = 64/65 (98.46%), Postives = 64/65 (98.46%), Query Frame = 1 
Query:    1 MEGTCTGEHGVGVGKIKYLPEEHGDGAMHAMRVIKRGLDPGGLMNPGKVLAHRRDPQTGRLVLCA 195
            MEGTCTGEHGVGVGKIKYLPEEHGDGAMH MRVIKRGLDPGGLMNPGKVLAHRRDPQTGRLVLCA
Sbjct:  369 MEGTCTGEHGVGVGKIKYLPEEHGDGAMHTMRVIKRGLDPGGLMNPGKVLAHRRDPQTGRLVLCA 433
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BLAST of mRNA_E-siliculosus-1a_F_contig1191.1225.1 vs. uniprot
Match: A0A835YUA6_9STRA (Putative d-lactate dehydrogenase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YUA6_9STRA)

HSP 1 Score: 93.2 bits (230), Expect = 1.140e-20
Identity = 42/64 (65.62%), Postives = 48/64 (75.00%), Query Frame = 1 
Query:    1 MEGTCTGEHGVGVGKIKYLPEEHGDGAMHAMRVIKRGLDPGGLMNPGKVLAHRRDPQTGRLVLC 192
            MEGTCTGEHGVG GKIKYL +E    A+  M  IK  +DP G+MNPGKV+ H+RDP TGRLV C
Sbjct:  432 MEGTCTGEHGVGYGKIKYLQKELNGAAIRTMVSIKNAIDPNGIMNPGKVVPHKRDPVTGRLVAC 495
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BLAST of mRNA_E-siliculosus-1a_F_contig1191.1225.1 vs. uniprot
Match: UPI0012389722 (FAD-binding protein n=1 Tax=Rhabdaerophilum calidifontis TaxID=2604328 RepID=UPI0012389722)

HSP 1 Score: 82.8 bits (203), Expect = 5.440e-17
Identity = 37/53 (69.81%), Postives = 44/53 (83.02%), Query Frame = 1 
Query:    1 MEGTCTGEHGVGVGKIKYLPEEHGDGAMHAMRVIKRGLDPGGLMNPGKVLAHR 159
            MEGTCTGEHG+G GK+KYL  E G GA+ AMRVIKRGLDP G++NPGK++  R
Sbjct:  414 MEGTCTGEHGIGQGKMKYLDAELGAGALEAMRVIKRGLDPEGILNPGKIVTLR 466
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BLAST of mRNA_E-siliculosus-1a_F_contig1191.1225.1 vs. uniprot
Match: UPI001ADC633B (FAD-binding protein n=1 Tax=Aquabacter sp. L1I39 TaxID=2820278 RepID=UPI001ADC633B)

HSP 1 Score: 82.4 bits (202), Expect = 7.500e-17
Identity = 36/51 (70.59%), Postives = 42/51 (82.35%), Query Frame = 1 
Query:    1 MEGTCTGEHGVGVGKIKYLPEEHGDGAMHAMRVIKRGLDPGGLMNPGKVLA 153
            MEGTCTGEHGVG GK+KYLP EHG+  + AMR IKR LDP  +MNPGK++A
Sbjct:  419 MEGTCTGEHGVGQGKMKYLPVEHGEATLDAMRAIKRALDPQDIMNPGKIVA 469
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BLAST of mRNA_E-siliculosus-1a_F_contig1191.1225.1 vs. uniprot
Match: A0A1R1ICB1_9RHOO (2-hydroxy-acid oxidase n=1 Tax=Azonexus hydrophilus TaxID=418702 RepID=A0A1R1ICB1_9RHOO)

HSP 1 Score: 80.1 bits (196), Expect = 4.880e-16
Identity = 35/50 (70.00%), Postives = 41/50 (82.00%), Query Frame = 1 
Query:    1 MEGTCTGEHGVGVGKIKYLPEEHGDGAMHAMRVIKRGLDPGGLMNPGKVL 150
            MEGTCTGEHG+G+GK KYL  EHG+ A+  MR +KR LDP GL+NPGKVL
Sbjct:  409 MEGTCTGEHGIGLGKRKYLVAEHGEVAVEVMRAVKRALDPAGLLNPGKVL 458
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BLAST of mRNA_E-siliculosus-1a_F_contig1191.1225.1 vs. uniprot
Match: Q47JF4_DECAR (FAD linked oxidase, C-terminal:FAD linked oxidase, N-terminal n=2 Tax=Azonexaceae TaxID=2008795 RepID=Q47JF4_DECAR)

HSP 1 Score: 80.1 bits (196), Expect = 4.910e-16
Identity = 35/50 (70.00%), Postives = 41/50 (82.00%), Query Frame = 1 
Query:    1 MEGTCTGEHGVGVGKIKYLPEEHGDGAMHAMRVIKRGLDPGGLMNPGKVL 150
            MEGTCTGEHG+G+GK KYLP EHGD A+  MR IK+ LDP  L+NPGK+L
Sbjct:  410 MEGTCTGEHGIGLGKRKYLPLEHGDVAVDVMRTIKQALDPNNLLNPGKIL 459
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BLAST of mRNA_E-siliculosus-1a_F_contig1191.1225.1 vs. uniprot
Match: A0A5C1ED71_9RHOO (D-lactate dehydrogenase n=1 Tax=Oryzomicrobium terrae TaxID=1735038 RepID=A0A5C1ED71_9RHOO)

HSP 1 Score: 80.1 bits (196), Expect = 4.950e-16
Identity = 35/50 (70.00%), Postives = 41/50 (82.00%), Query Frame = 1 
Query:    1 MEGTCTGEHGVGVGKIKYLPEEHGDGAMHAMRVIKRGLDPGGLMNPGKVL 150
            MEGTCTGEHG+G+GK K+L  EHG GA+  MRVIKR +DP  LMNPGK+L
Sbjct:  416 MEGTCTGEHGIGLGKQKFLVPEHGQGAVDLMRVIKRAIDPANLMNPGKIL 465
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BLAST of mRNA_E-siliculosus-1a_F_contig1191.1225.1 vs. uniprot
Match: UPI0015EEDDA2 (FAD-binding protein n=1 Tax=Chthonobacter albigriseus TaxID=1683161 RepID=UPI0015EEDDA2)

HSP 1 Score: 80.1 bits (196), Expect = 5.010e-16
Identity = 39/58 (67.24%), Postives = 45/58 (77.59%), Query Frame = 1 
Query:    1 MEGTCTGEHGVGVGKIKYLPEEHGDGAMHAMRVIKRGLDPGGLMNPGKVLAHRRDPQT 174
            M GTCTGEHGVG GKIKYLP EHG  A+  MR +K+ LDP G++NPGK+LA R DP T
Sbjct:  417 MGGTCTGEHGVGQGKIKYLPREHG-AALGLMRAVKQALDPLGILNPGKILA-RHDPDT 472
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BLAST of mRNA_E-siliculosus-1a_F_contig1191.1225.1 vs. uniprot
Match: A0A8H5MAJ0_9AGAR (FAD-binding PCMH-type domain-containing protein n=1 Tax=Tricholomella constricta TaxID=117010 RepID=A0A8H5MAJ0_9AGAR)

HSP 1 Score: 80.1 bits (196), Expect = 5.230e-16
Identity = 36/58 (62.07%), Postives = 44/58 (75.86%), Query Frame = 1 
Query:    1 MEGTCTGEHGVGVGKIKYLPEEHGDGAMHAMRVIKRGLDPGGLMNPGKVLAHRRDPQT 174
            +EGTCTGEHGVGVGK +YL +E G+G +  MRVIKR +DP  LMNPGK+   RR P+ 
Sbjct:  510 LEGTCTGEHGVGVGKKEYLTDELGEGTVELMRVIKRAIDPLNLMNPGKLYPERRKPKA 567
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BLAST of mRNA_E-siliculosus-1a_F_contig1191.1225.1 vs. uniprot
Match: A0A5J4E700_9BACT (Putative FAD-linked oxidoreductase n=1 Tax=bacterium MnTg03 TaxID=2080453 RepID=A0A5J4E700_9BACT)

HSP 1 Score: 74.7 bits (182), Expect = 1.210e-15
Identity = 31/50 (62.00%), Postives = 41/50 (82.00%), Query Frame = 1 
Query:    1 MEGTCTGEHGVGVGKIKYLPEEHGDGAMHAMRVIKRGLDPGGLMNPGKVL 150
            M GTCTGEHG+G+GK+K++  EHGDGA+  M+ IK+ LDP  +MNPGKV+
Sbjct:   79 MGGTCTGEHGIGIGKLKHMRNEHGDGAIATMQSIKQALDPHNIMNPGKVV 128
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig1191.1225.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G288_ECTSI3.810e-3898.46D-lactate dehydrogenase, putative n=1 Tax=Ectocarp... [more]
A0A835YUA6_9STRA1.140e-2065.63Putative d-lactate dehydrogenase n=1 Tax=Tribonema... [more]
UPI00123897225.440e-1769.81FAD-binding protein n=1 Tax=Rhabdaerophilum calidi... [more]
UPI001ADC633B7.500e-1770.59FAD-binding protein n=1 Tax=Aquabacter sp. L1I39 T... [more]
A0A1R1ICB1_9RHOO4.880e-1670.002-hydroxy-acid oxidase n=1 Tax=Azonexus hydrophilu... [more]
Q47JF4_DECAR4.910e-1670.00FAD linked oxidase, C-terminal:FAD linked oxidase,... [more]
A0A5C1ED71_9RHOO4.950e-1670.00D-lactate dehydrogenase n=1 Tax=Oryzomicrobium ter... [more]
UPI0015EEDDA25.010e-1667.24FAD-binding protein n=1 Tax=Chthonobacter albigris... [more]
A0A8H5MAJ0_9AGAR5.230e-1662.07FAD-binding PCMH-type domain-containing protein n=... [more]
A0A5J4E700_9BACT1.210e-1562.00Putative FAD-linked oxidoreductase n=1 Tax=bacteri... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig1191contigE-siliculosus-1a_F_contig1191:33142..34216 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start1
Stop1
Cds size198
Model size198
Exons2
Hectar predicted targeting categoryother localisation
EC1.1.2.4
Ec32 orthologEc-16_002060.1
Ec32 ortholog descriptionFAD-binding, type 2, subdomain 1
EggNOG OGsCOG0277@1,KOG1231@2759
EggNOG free text desc.cytokinin dehydrogenase activity
BRITEko00000,ko00001,ko01000
Best eggNOG OGNA|NA|NA
Best tax levelEukaryota
BiGG ReactioniMM904.YDL174C,iND750.YDL174C
COG Functional cat.C
GOsGO:0000166,GO:0003674,GO:0003824,GO:0003973,GO:0004457,GO:0004458,GO:0005488,GO:0005515,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005740,GO:0005743,GO:0005759,GO:0006081,GO:0006082,GO:0006089,GO:0008144,GO:0008150,GO:0008152,GO:0008720,GO:0008891,GO:0009056,GO:0009438,GO:0009987,GO:0016020,GO:0016054,GO:0016491,GO:0016614,GO:0016616,GO:0016898,GO:0016899,GO:0017076,GO:0019154,GO:0019752,GO:0019866,GO:0030554,GO:0031090,GO:0031966,GO:0031967,GO:0031974,GO:0031975,GO:0032553,GO:0032555,GO:0032559,GO:0032787,GO:0035639,GO:0036094,GO:0042180,GO:0042182,GO:0042802,GO:0043167,GO:0043168,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043436,GO:0044237,GO:0044248,GO:0044281,GO:0044282,GO:0044422,GO:0044424,GO:0044429,GO:0044444,GO:0044446,GO:0044464,GO:0046185,GO:0046395,GO:0048037,GO:0050660,GO:0050662,GO:0051596,GO:0055114,GO:0070013,GO:0071704,GO:0072329,GO:0097159,GO:0097367,GO:1901265,GO:1901363,GO:1901575,GO:1901615,GO:1901616,GO:1903457
KEGG Pathwayko00620,map00620
KEGG ReactionR00197
KEGG koko:K00102
KEGG rclassRC00044
Preferred nameDLD1
Seed eggNOG ortholog2880.D7G288
Seed ortholog evalue4.5e-31
Seed ortholog score139.8
Taxonomic scopeEukaryota
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927710.632664-CDS-E-siliculosus-1a_F_contig1191:33141..332781622927710.632664-CDS-E-siliculosus-1a_F_contig1191:33141..33278Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1191 33142..33278 -
1696318649.261797-CDS-E-siliculosus-1a_F_contig1191:33141..332781696318649.261797-CDS-E-siliculosus-1a_F_contig1191:33141..33278Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1191 33142..33278 -
1622927710.6440291-CDS-E-siliculosus-1a_F_contig1191:34155..342161622927710.6440291-CDS-E-siliculosus-1a_F_contig1191:34155..34216Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1191 34156..34216 -
1696318649.2744198-CDS-E-siliculosus-1a_F_contig1191:34155..342161696318649.2744198-CDS-E-siliculosus-1a_F_contig1191:34155..34216Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1191 34156..34216 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig1191.1225.1prot_E-siliculosus-1a_F_contig1191.1225.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig1191 33142..34216 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig1191.1225.1

>prot_E-siliculosus-1a_F_contig1191.1225.1 ID=prot_E-siliculosus-1a_F_contig1191.1225.1|Name=mRNA_E-siliculosus-1a_F_contig1191.1225.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=66bp
MEGTCTGEHGVGVGKIKYLPEEHGDGAMHAMRVIKRGLDPGGLMNPGKVL
AHRRDPQTGRLVLCA*
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mRNA from alignment at E-siliculosus-1a_F_contig1191:33142..34216-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig1191.1225.1 ID=mRNA_E-siliculosus-1a_F_contig1191.1225.1|Name=mRNA_E-siliculosus-1a_F_contig1191.1225.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=1075bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1191:33142..34216- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATGGAGGGCACTTGCACGGGCGAGCACGGAGTGGGTGTCGGGAAGATCAA
GTACTTGCCTGGTGAGGTTATTTGCCGGTTGCTGTGTTGATGGTGCAGTT
TTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTCCTGCTGCTGTT
GGTGTTGTTGGTGTTGGTTGTTGTTGTTGTTGTTGTTGCCTTTGTCGTGA
CTTGTCGCGAGTGGCGCAGGCGAGTTAGTTATCCTAGGTTCGATTAAGGT
CGTGTGCCAACAACGGGGATCTTCTCGGCAATTATGCATAGCAGCAGTAG
TAGAACAACATACCGATATGGACGGTTGGCCCACCTCGAGTATTCCTCGT
GCCAATGTGCACCTCGTGCCTGTCAGAGTCGGGCTCAAGCAACGCAAGCA
GCAATAATTTGTCGGAGAATTTCCGTGCACCTATGATAGGGTGGGGTTGT
TTCACCAGGACACGGTCGTCAGCCGCTTTTGGCCTCAACATAACCTCTTT
TCTCCCCTCCCACCCCATAGATACTTCGTTTCCGTCCGGGGATCTCGCTC
TGAGGCAAGGAATTCACCTCACGCAAAGCTTGATATTGTCAGCTATGAAC
TGGGGGGGGTCCGCAGTTGAGCGTGCGGTCGATCAAGTGTCTGGACTGCT
CCATGGCGTACTTTTTTCGTTTTCTTGAAGCCTCGTGGCGTATCTTATTT
CGTTCGTTCGACATCAGGTCCTTGTCTCGCAAGAATCGACATCCAACGTT
GCCCGTCCGCCCTCGCCCTCGTCCGCCCACCCGACCGTGTCCGCCCGCAT
GTCGCTCTCGGCCTGTTCTCTTTGCCCCATCGCTCGACCACTCAAATAAC
GAAAACCTTGCGCTCAACAACAACCACAACGACAACGACAAATGGCGTTA
TCAACAACAATAACAATAACGACAACAACGATCACCAGAGGAGCATGGGG
ACGGCGCCATGCACGCGATGAGGGTCATCAAGCGGGGGCTTGATCCCGGA
GGCCTTATGAACCCGGGCAAAGTGCTAGCGCATCGGAGGGACCCCCAGAC
GGGCCGTTTGGTCCTCTGCGCTTAA
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig1191:33142..34216-

>mRNA_E-siliculosus-1a_F_contig1191.1225.1 ID=mRNA_E-siliculosus-1a_F_contig1191.1225.1|Name=mRNA_E-siliculosus-1a_F_contig1191.1225.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=396bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1191:33142..34216- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATGGAGGGCACTTGCACGGGCGAGCACGGAGTGGGTGTCGGGAAGATCAA
GTACTTGCCTGATGGAGGGCACTTGCACGGGCGAGCACGGAGTGGGTGTC
GGGAAGATCAAGTACTTGCCTGAGGAGCATGGGGACGGCGCCATGCACGC
GATGAGGGTCATCAAGCGGGGGCTTGATCCCGGAGGCCTTATGAACCCGG
GCAAAGTGCTAGCGCATCGGAGGGACCCCCAGACGGGCCGTTTGGTCCTC
TGCGCTTAAAGGAGCATGGGGACGGCGCCATGCACGCGATGAGGGTCATC
AAGCGGGGGCTTGATCCCGGAGGCCTTATGAACCCGGGCAAAGTGCTAGC
GCATCGGAGGGACCCCCAGACGGGCCGTTTGGTCCTCTGCGCTTAA
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