mRNA_E-siliculosus-1a_F_contig1150.985.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig1150.985.1
Unique NamemRNA_E-siliculosus-1a_F_contig1150.985.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig1150.985.1 vs. uniprot
Match: A0A6H5KQ62_9PHAE (Reverse transcriptase domain-containing protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KQ62_9PHAE)

HSP 1 Score: 393 bits (1009), Expect = 3.280e-135
Identity = 196/199 (98.49%), Postives = 198/199 (99.50%), Query Frame = 1 
Query:    1 RQRRATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIKAAGDRLKTHKIDSVAEADEPFEIKFIKQTVKKANPQSAPGPDGLRFSYLQAGGMSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE 597
            RQRRATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIKAAGDR++THKIDSVAE DEPFEIKFIKQTVKKANPQSAPGPDGLRFSYLQAGGMSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE
Sbjct:   41 RQRRATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIKAAGDRIETHKIDSVAEPDEPFEIKFIKQTVKKANPQSAPGPDGLRFSYLQAGGMSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE 239
[Close]
BLAST of mRNA_E-siliculosus-1a_F_contig1150.985.1 vs. uniprot
Match: A0A6H5KGJ4_9PHAE (Reverse transcriptase domain-containing protein n=3 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KGJ4_9PHAE)

HSP 1 Score: 394 bits (1013), Expect = 1.040e-132
Identity = 197/199 (98.99%), Postives = 198/199 (99.50%), Query Frame = 1 
Query:    1 RQRRATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIKAAGDRLKTHKIDSVAEADEPFEIKFIKQTVKKANPQSAPGPDGLRFSYLQAGGMSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE 597
            RQRRATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIKAAGDR+KTHKIDSVAEADEPFEIKFIKQTVKKANPQSAPGPDGLRFSYLQAGGMSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFC QYKGGIANIFEAANQFGGGVPGGVE
Sbjct:   41 RQRRATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIKAAGDRIKTHKIDSVAEADEPFEIKFIKQTVKKANPQSAPGPDGLRFSYLQAGGMSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCHQYKGGIANIFEAANQFGGGVPGGVE 239
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BLAST of mRNA_E-siliculosus-1a_F_contig1150.985.1 vs. uniprot
Match: A0A6H5JCM0_9PHAE (Reverse transcriptase domain-containing protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JCM0_9PHAE)

HSP 1 Score: 396 bits (1018), Expect = 9.240e-131
Identity = 198/199 (99.50%), Postives = 199/199 (100.00%), Query Frame = 1 
Query:    1 RQRRATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIKAAGDRLKTHKIDSVAEADEPFEIKFIKQTVKKANPQSAPGPDGLRFSYLQAGGMSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE 597
            RQRRATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIKAAGDR+KTHKIDSVAEADEPFEIKFIKQTVKKANPQSAPGPDGLRFSYLQAGGMSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE
Sbjct:   41 RQRRATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIKAAGDRIKTHKIDSVAEADEPFEIKFIKQTVKKANPQSAPGPDGLRFSYLQAGGMSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE 239
[Close]
BLAST of mRNA_E-siliculosus-1a_F_contig1150.985.1 vs. uniprot
Match: A0A6H5LN63_9PHAE (Reverse transcriptase domain-containing protein n=3 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5LN63_9PHAE)

HSP 1 Score: 392 bits (1006), Expect = 1.040e-126
Identity = 196/199 (98.49%), Postives = 197/199 (98.99%), Query Frame = 1 
Query:    1 RQRRATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIKAAGDRLKTHKIDSVAEADEPFEIKFIKQTVKKANPQSAPGPDGLRFSYLQAGGMSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE 597
            RQRRATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIK AGDR+KTHKIDSVAEADEPFEIKFIKQTVKKANPQSAPGPDGLRFSYLQAGGMSD FTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE
Sbjct:   41 RQRRATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIKVAGDRIKTHKIDSVAEADEPFEIKFIKQTVKKANPQSAPGPDGLRFSYLQAGGMSDSFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE 239
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BLAST of mRNA_E-siliculosus-1a_F_contig1150.985.1 vs. uniprot
Match: A0A6H5KSY9_9PHAE (Reverse transcriptase domain-containing protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KSY9_9PHAE)

HSP 1 Score: 177 bits (448), Expect = 1.370e-49
Identity = 86/87 (98.85%), Postives = 87/87 (100.00%), Query Frame = 1 
Query:  337 MSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE 597
            MSD+FTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE
Sbjct:    1 MSDYFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE 87
[Close]
BLAST of mRNA_E-siliculosus-1a_F_contig1150.985.1 vs. uniprot
Match: A0A6H5K1H0_9PHAE (Reverse transcriptase domain-containing protein n=2 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K1H0_9PHAE)

HSP 1 Score: 168 bits (426), Expect = 8.920e-45
Identity = 84/87 (96.55%), Postives = 84/87 (96.55%), Query Frame = 1 
Query:  337 MSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE 597
            MSD FTETLADFSFKVFYYGDELPDF WNLHTSANLSALGEKKRPIAVGGVLRRIISGSF RQYKGGIANIFEAANQFGGGVPGGVE
Sbjct:    1 MSDSFTETLADFSFKVFYYGDELPDFLWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFYRQYKGGIANIFEAANQFGGGVPGGVE 87
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BLAST of mRNA_E-siliculosus-1a_F_contig1150.985.1 vs. uniprot
Match: A0A6H5L3H3_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L3H3_9PHAE)

HSP 1 Score: 154 bits (390), Expect = 2.060e-44
Identity = 76/87 (87.36%), Postives = 81/87 (93.10%), Query Frame = 1 
Query:  337 MSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE 597
            MSD F+ETLADFS+KVFYYGDELPD FW   TSA+LSALGEKKRPIAVGGVLRRIISGSFCRQYK GIA+IFEA+NQFGGGVPGGVE
Sbjct:    1 MSDDFSETLADFSYKVFYYGDELPDLFWGFQTSASLSALGEKKRPIAVGGVLRRIISGSFCRQYKQGIADIFEASNQFGGGVPGGVE 87
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BLAST of mRNA_E-siliculosus-1a_F_contig1150.985.1 vs. uniprot
Match: A0A6P0N5L4_9CYAN (Patatin-like protein n=1 Tax=Moorena sp. SIO3C2 TaxID=2607842 RepID=A0A6P0N5L4_9CYAN)

HSP 1 Score: 160 bits (405), Expect = 6.190e-42
Identity = 90/197 (45.69%), Postives = 123/197 (62.44%), Query Frame = 1 
Query:   10 RATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAW-DSSIKAAGDRLKTHKIDSVAEADEPFEIKFIKQTVKKANPQSAPGPDGLRFSYLQAGGMSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE 597
            RA  LAH+ GG++KAAS L++P   PRN +TLE LR  HP E   +IA A  +++ + +       +I++V   +EPF  + +  T+++ NPQ +PGP GLRFS LQ+  ++    E +A     V + G  LPD FW LHTSANLSA+G+K RPIAVG + RRI+ G  CRQY   +A  FE   Q+G  VPGG E
Sbjct: 1084 RAEHLAHQRGGLSKAASTLLAPPSPPRNAETLETLRKKHPGESQQEIAEACCEATARLSRPPPGVSRIEAV---EEPFATETVTATIRRGNPQGSPGPSGLRFSLLQSS-LTPELCEAIAKLC-SVIFDGSHLPDSFWQLHTSANLSAIGDKCRPIAVGDLFRRIVGGVICRQYGPSLAEHFEPQGQYGVAVPGGTE 1275
[Close]
BLAST of mRNA_E-siliculosus-1a_F_contig1150.985.1 vs. uniprot
Match: A0A6H5KQE6_9PHAE (Uncharacterized protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KQE6_9PHAE)

HSP 1 Score: 146 bits (369), Expect = 1.950e-41
Identity = 77/99 (77.78%), Postives = 81/99 (81.82%), Query Frame = 1 
Query:  337 MSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSALGEKKRPIAVGGVLRRII------------SGSFCRQYKGGIANIFEAANQFGGGVPGGVE 597
            MSD F+ TLADFS KVFYYGDELPD FW LHTSA+LSALGEKKRPIAVGGVLRRII            SGSFCRQYK GIA+IFEA+NQFGGGVPGGVE
Sbjct:    1 MSDDFSATLADFSHKVFYYGDELPDLFWGLHTSASLSALGEKKRPIAVGGVLRRIIXIXXXXXXXXXISGSFCRQYKQGIADIFEASNQFGGGVPGGVE 99
[Close]
BLAST of mRNA_E-siliculosus-1a_F_contig1150.985.1 vs. uniprot
Match: A0A6H5K8P4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K8P4_9PHAE)

HSP 1 Score: 138 bits (348), Expect = 3.670e-38
Identity = 69/77 (89.61%), Postives = 72/77 (93.51%), Query Frame = 1 
Query:    1 RQRRATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIKAAGDRLKTHKIDSVAEAD 231
            RQRRATRL HE GGIT+ ASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIKAAGD +KT +IDSVAEAD
Sbjct:   92 RQRRATRLVHERGGITEVASALISPAPSPRNNDTLEHLRNLHPNEDPDDIATAWDSSIKAAGDSIKTRRIDSVAEAD 168
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig1150.985.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 12
Match NameE-valueIdentityDescription
A0A6H5KQ62_9PHAE3.280e-13598.49Reverse transcriptase domain-containing protein (F... [more]
A0A6H5KGJ4_9PHAE1.040e-13298.99Reverse transcriptase domain-containing protein n=... [more]
A0A6H5JCM0_9PHAE9.240e-13199.50Reverse transcriptase domain-containing protein (F... [more]
A0A6H5LN63_9PHAE1.040e-12698.49Reverse transcriptase domain-containing protein n=... [more]
A0A6H5KSY9_9PHAE1.370e-4998.85Reverse transcriptase domain-containing protein (F... [more]
A0A6H5K1H0_9PHAE8.920e-4596.55Reverse transcriptase domain-containing protein n=... [more]
A0A6H5L3H3_9PHAE2.060e-4487.36Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6P0N5L4_9CYAN6.190e-4245.69Patatin-like protein n=1 Tax=Moorena sp. SIO3C2 Ta... [more]
A0A6H5KQE6_9PHAE1.950e-4177.78Uncharacterized protein (Fragment) n=1 Tax=Ectocar... [more]
A0A6H5K8P4_9PHAE3.670e-3889.61Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig1150contigE-siliculosus-1a_F_contig1150:2..598 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start0
Stop0
Cds size597
Model size597
Exons1
Hectar predicted targeting categoryother localisation
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927664.714166-CDS-E-siliculosus-1a_F_contig1150:1..5981622927664.714166-CDS-E-siliculosus-1a_F_contig1150:1..598Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1150 2..598 -
1696318630.3696313-CDS-E-siliculosus-1a_F_contig1150:1..5981696318630.3696313-CDS-E-siliculosus-1a_F_contig1150:1..598Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig1150 2..598 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig1150.985.1prot_E-siliculosus-1a_F_contig1150.985.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig1150 2..598 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig1150.985.1

>prot_E-siliculosus-1a_F_contig1150.985.1 ID=prot_E-siliculosus-1a_F_contig1150.985.1|Name=mRNA_E-siliculosus-1a_F_contig1150.985.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=199bp
RQRRATRLAHESGGITKAASALISPAPSPRNNDTLEHLRNLHPNEDPDDI
ATAWDSSIKAAGDRLKTHKIDSVAEADEPFEIKFIKQTVKKANPQSAPGP
DGLRFSYLQAGGMSDFFTETLADFSFKVFYYGDELPDFFWNLHTSANLSA
LGEKKRPIAVGGVLRRIISGSFCRQYKGGIANIFEAANQFGGGVPGGVE
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mRNA from alignment at E-siliculosus-1a_F_contig1150:2..598-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig1150.985.1 ID=mRNA_E-siliculosus-1a_F_contig1150.985.1|Name=mRNA_E-siliculosus-1a_F_contig1150.985.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=597bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1150:2..598- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
CGCCAAAGAAGAGCCACAAGACTTGCGCACGAAAGCGGAGGCATCACTAA
AGCTGCCAGTGCCTTAATCTCCCCAGCACCTTCACCGCGCAACAACGACA
CGCTGGAGCATCTTCGCAACCTACACCCTAACGAAGATCCTGACGATATT
GCTACTGCGTGGGATTCCAGCATCAAAGCTGCGGGAGATCGTCTAAAAAC
GCACAAGATAGACTCCGTCGCCGAAGCTGATGAACCATTCGAAATCAAGT
TCATCAAGCAGACGGTAAAGAAAGCCAACCCTCAGAGTGCTCCGGGGCCT
GATGGTCTTCGTTTTTCGTATCTCCAAGCTGGTGGGATGAGTGATTTTTT
TACTGAAACCTTGGCGGACTTCAGCTTTAAAGTCTTCTACTACGGAGACG
AGCTTCCGGATTTCTTTTGGAACCTCCACACCAGTGCCAACCTTTCAGCG
CTGGGAGAGAAAAAACGGCCAATTGCAGTTGGAGGGGTTTTGCGTCGCAT
CATCAGCGGCAGCTTCTGCCGCCAGTACAAGGGAGGAATTGCGAACATCT
TCGAAGCAGCAAATCAGTTCGGAGGTGGTGTTCCAGGAGGAGTTGAG
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig1150:2..598-

>mRNA_E-siliculosus-1a_F_contig1150.985.1 ID=mRNA_E-siliculosus-1a_F_contig1150.985.1|Name=mRNA_E-siliculosus-1a_F_contig1150.985.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=1194bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig1150:2..598- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
CGCCAAAGAAGAGCCACAAGACTTGCGCACGAAAGCGGAGGCATCACTAA
AGCTGCCAGTGCCTTAATCTCCCCAGCACCTTCACCGCGCAACAACGACA
CGCTGGAGCATCTTCGCAACCTACACCCTAACGAAGATCCTGACGATATT
GCTACTGCGTGGGATTCCAGCATCAAAGCTGCGGGAGATCGTCTAAAAAC
GCACAAGATAGACTCCGTCGCCGAAGCTGATGAACCATTCGAAATCAAGT
TCATCAAGCAGACGGTAAAGAAAGCCAACCCTCAGAGTGCTCCGGGGCCT
GATGGTCTTCGTTTTTCGTATCTCCAAGCTGGTGGGATGAGTGATTTTTT
TACTGAAACCTTGGCGGACTTCAGCTTTAAAGTCTTCTACTACGGAGACG
AGCTTCCGGATTTCTTTTGGAACCTCCACACCAGTGCCAACCTTTCAGCG
CTGGGAGAGAAAAAACGGCCAATTGCAGTTGGAGGGGTTTTGCGTCGCAT
CATCAGCGGCAGCTTCTGCCGCCAGTACAAGGGAGGAATTGCGAACATCT
TCGAAGCAGCAAATCAGTTCGGAGGTGGTGTTCCAGGAGGAGTTGAGCGC
CAAAGAAGAGCCACAAGACTTGCGCACGAAAGCGGAGGCATCACTAAAGC
TGCCAGTGCCTTAATCTCCCCAGCACCTTCACCGCGCAACAACGACACGC
TGGAGCATCTTCGCAACCTACACCCTAACGAAGATCCTGACGATATTGCT
ACTGCGTGGGATTCCAGCATCAAAGCTGCGGGAGATCGTCTAAAAACGCA
CAAGATAGACTCCGTCGCCGAAGCTGATGAACCATTCGAAATCAAGTTCA
TCAAGCAGACGGTAAAGAAAGCCAACCCTCAGAGTGCTCCGGGGCCTGAT
GGTCTTCGTTTTTCGTATCTCCAAGCTGGTGGGATGAGTGATTTTTTTAC
TGAAACCTTGGCGGACTTCAGCTTTAAAGTCTTCTACTACGGAGACGAGC
TTCCGGATTTCTTTTGGAACCTCCACACCAGTGCCAACCTTTCAGCGCTG
GGAGAGAAAAAACGGCCAATTGCAGTTGGAGGGGTTTTGCGTCGCATCAT
CAGCGGCAGCTTCTGCCGCCAGTACAAGGGAGGAATTGCGAACATCTTCG
AAGCAGCAAATCAGTTCGGAGGTGGTGTTCCAGGAGGAGTTGAG
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