mRNA_E-fasciculatus_F_contig14908.3492.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig14908.3492.1
Unique NamemRNA_E-fasciculatus_F_contig14908.3492.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig14908.3492.1 vs. uniprot
Match: A0A6H5KEJ8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KEJ8_9PHAE)

HSP 1 Score: 155 bits (393), Expect = 4.210e-44
Identity = 74/78 (94.87%), Postives = 75/78 (96.15%), Query Frame = 1 
Query:   22 IHLGSPCILPTTAPVSFLDLRDLTELLQDGGHADLVSLETWSRDAGAYYCNEMFYRTLHAIRELGTPSVDGSSLIPAS 255
            IH GSPCILPTTAPVSFLDL DL ELLQDGGHADLV+LETWSRDAGAYYCNEMFYRTLHAIRELGTPSVDGSSLIPAS
Sbjct:  177 IHEGSPCILPTTAPVSFLDLGDLAELLQDGGHADLVTLETWSRDAGAYYCNEMFYRTLHAIRELGTPSVDGSSLIPAS 254
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BLAST of mRNA_E-fasciculatus_F_contig14908.3492.1 vs. uniprot
Match: A0A7S3EUW8_9EUKA (Hypothetical protein n=1 Tax=Haptolina ericina TaxID=156174 RepID=A0A7S3EUW8_9EUKA)

HSP 1 Score: 71.6 bits (174), Expect = 3.910e-13
Identity = 48/119 (40.34%), Postives = 64/119 (53.78%), Query Frame = 1 
Query:   31 GSPCILPTTAPVSFLDLRDLTELLQDGG----------HADLVSLETWSRDAGAYYCNEMFYRTLHAIRELGT-PSVDGSSLIP--ASFFSAMFVHLPDVAVMGLDDMAVMVSFVAQRI 348
            G+ C+L TTAP+  ++L  L   L+DGG           A  + LE WSRDAG YYCNE+++RTL+ IR  G  P     SL    AS   A+FVHLP   V   +  A  +  V ++I
Sbjct:   40 GAACLLGTTAPLDRINLP-LPAHLRDGGLAIQTLSLQQEAPRLPLELWSRDAGTYYCNEVYFRTLNVIRRAGLRPHYHPLSLASHGASLLPALFVHLPAAEVSTDEQSAEFIWQVIEKI 157
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BLAST of mRNA_E-fasciculatus_F_contig14908.3492.1 vs. uniprot
Match: A0A7S4VFG5_9DINO (Hypothetical protein n=2 Tax=Alexandrium monilatum TaxID=311494 RepID=A0A7S4VFG5_9DINO)

HSP 1 Score: 72.4 bits (176), Expect = 2.190e-12
Identity = 43/112 (38.39%), Postives = 60/112 (53.57%), Query Frame = 1 
Query:   22 IHLGSPCILPTTAPVSFLDLRDLTELLQDGGHADLVSLETWSRDAGAYYCNEMFYRTLHAIRELGTPSVDGSS-LIPASFFSAMFVHLPDVAVMGLDDMAVMVSFVAQRIML 354
            + +  PC+L +TAP++ L+L  L               E WSRDAGAYYCNE++YRTL A+R    P   G   L+P      +FVHLP   V  +++ A  V  VA R+ +
Sbjct:  237 LDVRGPCLLASTAPLNGLELPLLRRFPSPR--------EIWSRDAGAYYCNEVYYRTLQAVRTRLIPPASGQQDLLPV-----IFVHLPKPEVSSIEESAKFVQDVADRLSI 335
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BLAST of mRNA_E-fasciculatus_F_contig14908.3492.1 vs. uniprot
Match: A0A7S2W0K8_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2W0K8_9STRA)

HSP 1 Score: 65.1 bits (157), Expect = 5.920e-11
Identity = 41/95 (43.16%), Postives = 56/95 (58.95%), Query Frame = 1 
Query:   34 SPCILPTTAPVSFLDLRDLTELLQDGGHADLVSLETWSRDAGAYYCNEMFYRTLHAIRELGTPSVDGSSLIPASFFSAMFVHLPDVAV-MGLDDM 315
            +P +LPTT  + ++ LR L +      H   V+ E WSRDAGAYYCNE++YR+L  +R     S D S +IP      +F+HLP     +GLDDM
Sbjct:   18 APHLLPTTVRLDYVTLRRLQQETASE-HLRRVT-ELWSRDAGAYYCNELYYRSLETVRR----SRDSSWVIPV-----LFIHLPTPDPDLGLDDM 101
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BLAST of mRNA_E-fasciculatus_F_contig14908.3492.1 vs. uniprot
Match: A0A7S4BFQ2_CHRCT (Hypothetical protein n=1 Tax=Chrysotila carterae TaxID=13221 RepID=A0A7S4BFQ2_CHRCT)

HSP 1 Score: 65.5 bits (158), Expect = 7.940e-10
Identity = 36/85 (42.35%), Postives = 48/85 (56.47%), Query Frame = 1 
Query:   31 GSPCILPTTAPVSFLDLRDLTELLQDGGHADLVSLETWSRDAGAYYCNEMFYRTLHAIRELGTPSVDGSSLIPASFFSAMFVHLP 285
            G+ C++PTTAP     +  LT  L    H  L   E WSRDAG+YYCNE+++RTLHA+R     +  G     A     +FVH+P
Sbjct:  351 GAACLVPTTAP-----MHKLTLPLLRRPHR-LEQEEIWSRDAGSYYCNEVYFRTLHAVRSKQIGAATGR----AGLLPVVFVHMP 425
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BLAST of mRNA_E-fasciculatus_F_contig14908.3492.1 vs. uniprot
Match: A0A7S2VZR4_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2VZR4_9STRA)

HSP 1 Score: 65.1 bits (157), Expect = 8.060e-10
Identity = 41/95 (43.16%), Postives = 56/95 (58.95%), Query Frame = 1 
Query:   34 SPCILPTTAPVSFLDLRDLTELLQDGGHADLVSLETWSRDAGAYYCNEMFYRTLHAIRELGTPSVDGSSLIPASFFSAMFVHLPDVAV-MGLDDM 315
            +P +LPTT  + ++ LR L +      H   V+ E WSRDAGAYYCNE++YR+L  +R     S D S +IP      +F+HLP     +GLDDM
Sbjct:  208 APHLLPTTVRLDYVTLRRLQQETASE-HLRRVT-ELWSRDAGAYYCNELYYRSLETVRR----SRDSSWVIPV-----LFIHLPTPDPDLGLDDM 291
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BLAST of mRNA_E-fasciculatus_F_contig14908.3492.1 vs. uniprot
Match: A0A7S3JRC4_9STRA (Hypothetical protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3JRC4_9STRA)

HSP 1 Score: 61.6 bits (148), Expect = 1.570e-8
Identity = 44/125 (35.20%), Postives = 58/125 (46.40%), Query Frame = 1 
Query:    4 TRWDGIIHLGSPCILPTTAPVSFLDLRDLTELLQDGGHADLVSLETWSRDAGAYYCNEMFYRTLHAIR--ELGTPS------------VDGSSLIPAS---------FFSAMFVHLPDVAVMGLD 309
            T+    I  G PC+  TTAP+  L L    E           + E WSRDAGA+YCNE+++R+LH +R  +L  PS            +D  SL  AS             +FVHLPD  +M  D
Sbjct:  180 TKESAAIENG-PCLQVTTAPLDRLILPSTNE-----------TNEVWSRDAGAFYCNELYFRSLHTVRSRQLLVPSPSSSCLLLSQQLLDNDSLTRASDHHLHFAQALIPVLFVHLPDTNIMDND 292
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BLAST of mRNA_E-fasciculatus_F_contig14908.3492.1 vs. uniprot
Match: A0A7S2Z4Q2_9CHLO (Hypothetical protein n=1 Tax=Chloropicon laureae TaxID=464258 RepID=A0A7S2Z4Q2_9CHLO)

HSP 1 Score: 58.2 bits (139), Expect = 9.860e-8
Identity = 47/156 (30.13%), Postives = 61/156 (39.10%), Query Frame = 1 
Query:    1 PTRWDGIIHLG----------------------------SPCILPTTAPVSFLDLRDLTELLQDGGHADLVSLETWSRDAGAYYCNEMFYRTLHAIRELGTPSVDGSSLI----------PASFFSAMFVHLP-DVAVMGLDDMAVMVSFVAQRIM 351
            P+ W  ++H+G                            +P ILPTT         DL +L  D   A   + E WSRDAG YYCNE+FYR+LHA+R                       P      +FVHLP D     +  MA MV+ +   IM
Sbjct:   39 PSNWRAVVHMGLEDRAKGLKIEVAALNNRASDNTTVYPTAPPILPTTV--------DLGKLALDPTQA---TGEIWSRDAGHYYCNEIFYRSLHAVRRAAAXXXXXXXXXXXXXXXXXXXPRQLLPVVFVHLPEDTGGNTIAHMADMVTTIIATIM 183
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BLAST of mRNA_E-fasciculatus_F_contig14908.3492.1 vs. uniprot
Match: A0A5B8MWB4_9CHLO (Uncharacterized protein n=1 Tax=Chloropicon primus TaxID=1764295 RepID=A0A5B8MWB4_9CHLO)

HSP 1 Score: 57.4 bits (137), Expect = 3.030e-7
Identity = 41/123 (33.33%), Postives = 52/123 (42.28%), Query Frame = 1 
Query:    1 PTRWDGIIHLG----------------------------SPCILPTTAPVSFLDLRDLTELLQDGGHADLVSLETWSRDAGAYYCNEMFYRTLHAIRELGTPSVDGSSLIPASFFSAMFVHLP 285
            P  W+ IIHLG                               ILPTT  +  LDL      + DG   +    E WSRDAG +YCNE+FYRTL  +R +    +    L+P      +FVHLP
Sbjct:   98 PKAWEAIIHLGLESTTKGLKVEVAALNNKAKDNSTIIDGGSTILPTTVDLGALDLAPALSGV-DGSSPE----EMWSRDAGHFYCNEIFYRTLRVVRRM---YMKDGRLMPV-----VFVHLP 207
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BLAST of mRNA_E-fasciculatus_F_contig14908.3492.1 vs. uniprot
Match: A0A812X286_SYMPI (Pcp protein (Fragment) n=1 Tax=Symbiodinium pilosum TaxID=2952 RepID=A0A812X286_SYMPI)

HSP 1 Score: 54.3 bits (129), Expect = 9.660e-7
Identity = 35/99 (35.35%), Postives = 52/99 (52.53%), Query Frame = 1 
Query:   16 GIIHLGSPCILPTTAPVSFLDLRDLTELLQDGGHADLVSLETWSRDAGAYYCNEMFYRTLHAIRELGTPSVDGSSLIPASFFSAMFVHLPDVAVMGLDD 312
            G + +G P +LPTT  +  + L           H      E WSRDAG ++CNE++YRTL +IRE       G+ LIP+     +F+H+P +  M L +
Sbjct:   48 GRVVVGGPQLLPTTGDLGAVALNT------QNPH------ELWSRDAGDFFCNEIYYRTLFSIREHRRLRCPGA-LIPS-----IFIHVPPLHKMPLQE 128
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig14908.3492.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 18
Match NameE-valueIdentityDescription
A0A6H5KEJ8_9PHAE4.210e-4494.87Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A7S3EUW8_9EUKA3.910e-1340.34Hypothetical protein n=1 Tax=Haptolina ericina Tax... [more]
A0A7S4VFG5_9DINO2.190e-1238.39Hypothetical protein n=2 Tax=Alexandrium monilatum... [more]
A0A7S2W0K8_9STRA5.920e-1143.16Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A7S4BFQ2_CHRCT7.940e-1042.35Hypothetical protein n=1 Tax=Chrysotila carterae T... [more]
A0A7S2VZR4_9STRA8.060e-1043.16Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A7S3JRC4_9STRA1.570e-835.20Hypothetical protein n=1 Tax=Aureoumbra lagunensis... [more]
A0A7S2Z4Q2_9CHLO9.860e-830.13Hypothetical protein n=1 Tax=Chloropicon laureae T... [more]
A0A5B8MWB4_9CHLO3.030e-733.33Uncharacterized protein n=1 Tax=Chloropicon primus... [more]
A0A812X286_SYMPI9.660e-735.35Pcp protein (Fragment) n=1 Tax=Symbiodinium pilosu... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig14908contigE-fasciculatus_F_contig14908:1291..3182 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score65.9
Seed ortholog evalue1.7e-08
Seed eggNOG ortholog192875.XP_004348153.1
EggNOG OGs2E8W4@1,2SFAX@2759
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionPeptidase C15, pyroglutamyl peptidase I-like
Ec32 orthologEc-06_002440.1
Exons3
Model size396
Cds size396
Stop1
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig14908.3492.1prot_E-fasciculatus_F_contig14908.3492.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig14908 1291..3182 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927971.8303566-CDS-E-fasciculatus_F_contig14908:1290..14221622927971.8303566-CDS-E-fasciculatus_F_contig14908:1290..1422Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig14908 1291..1422 -
1695995773.137922-CDS-E-fasciculatus_F_contig14908:1290..14221695995773.137922-CDS-E-fasciculatus_F_contig14908:1290..1422Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig14908 1291..1422 -
1622927971.8437476-CDS-E-fasciculatus_F_contig14908:2016..22501622927971.8437476-CDS-E-fasciculatus_F_contig14908:2016..2250Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig14908 2017..2250 -
1695995773.1501594-CDS-E-fasciculatus_F_contig14908:2016..22501695995773.1501594-CDS-E-fasciculatus_F_contig14908:2016..2250Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig14908 2017..2250 -
1622927971.8571522-CDS-E-fasciculatus_F_contig14908:3152..31821622927971.8571522-CDS-E-fasciculatus_F_contig14908:3152..3182Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig14908 3153..3182 -
1695995773.1600282-CDS-E-fasciculatus_F_contig14908:3152..31821695995773.1600282-CDS-E-fasciculatus_F_contig14908:3152..3182Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig14908 3153..3182 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig14908.3492.1

>prot_E-fasciculatus_F_contig14908.3492.1 ID=prot_E-fasciculatus_F_contig14908.3492.1|Name=mRNA_E-fasciculatus_F_contig14908.3492.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=132bp
PTRWDGIIHLGSPCILPTTAPVSFLDLRDLTELLQDGGHADLVSLETWSR
DAGAYYCNEMFYRTLHAIRELGTPSVDGSSLIPASFFSAMFVHLPDVAVM
GLDDMAVMVSFVAQRIMLGTVGLLPDACSDR*
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mRNA from alignment at E-fasciculatus_F_contig14908:1291..3182-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig14908.3492.1 ID=mRNA_E-fasciculatus_F_contig14908.3492.1|Name=mRNA_E-fasciculatus_F_contig14908.3492.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=1892bp|location=Sequence derived from alignment at E-fasciculatus_F_contig14908:1291..3182- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
CCCACGAGATGGGACGGCATCATCCACTTGGTGTGTGTGGGCTCCACTCC
GTCTTCGTTTCGCTGTTGTGTAACCTTTGGGATTGGTATGTGTTGTAGCA
GCAGTTGTTCCTGGCGCGTGGACACGTGGCTAGGGGAGCGTGCGTTTCGG
AGAGGCTAAGCACGTACGTTCTTTGTATATTCGTGCCCACAACCCTCGCC
GCAGCTGCTCGCACGAAATGCGCTGAGTACCAGCAGGTTGGTTTGTACCT
TCGTAGCTTTCATAGAAACTAAGCTCACGGCGCGTGTTTGTGGACACTAT
AGTGTTGTCTGGGTATTTGCTGACGATCCATCGTTAACTTCCAATAACAG
TGATTTTTCACCTGTTTGTATCACGGTTGTATTTCGTATAACTCCTAGAC
TCCTCTCACTTCTCCCCCTCTTAATCTCCTCTCCTTTGTTGGTTGGTTGC
GGATGACCGGATGACTTCTTCTCCGTGGGAAGGGCTTCGAGTCGTCGGCG
AAGGGTCTTCGGCTGGAAACGATAGCGGCCAACGTCAAAACTTCGGACGA
CTACCACTACCTGTGGAACTCGGACATCCCGTGCCAGAAGGAAGGCACCC
CTTTCGAGTATATCCATGAGGTTGGGTGGACAGTTAACGTAGGATTTCTG
TGTCGACCGATCGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
GAGAGACCGACAGAGGGACAGCGAGACAGAGAGATACTGTAGAGGCAGAG
ACTAACAAGCGGCCTACGACAAAATTACTCGGAATACAAACTCACCATAT
ACCTGTTCGAGTTTGTCCCTCAACTCCCCACATGTCTTTAACAACAGACC
CCCCCCCCGCGTTTCCCGATCCAATCATGCCTAAACCTTCATGTGTGCGG
CACCCCCGCGCTGCTTCGTCCCCTCCGCGCAGGGCTCCCCCTGCATTCTC
CCCACCACGGCGCCCGTGTCCTTCCTCGATCTCCGTGACCTCACGGAACT
CCTGCAAGACGGCGGGCACGCCGACCTCGTGTCTCTGGAGACTTGGAGCA
GGGACGCCGGGGCGTACTACTGCAACGAGATGTTCTACCGCACGCTGCAC
GCCATTCGCGAGCTGGGCACCCCGTCGGTAGATGGATCTTCTCTTATCCC
GGCGAGTTTTTTTTCCGTGTTTGTTGGGGTTGAGGGGGGAGAAAGATGAA
GCAGGTGACAGACGACCGTAAATTGCTTGCGGTAGGTAGTCTTGCGGACA
GGCGTGTACGTAGACGGGGGCGAACTCGCTCCAAGCCCGGGTTCTCGCGG
AGGGAATACGCTTGTTGGTTTTGTTTCGATAGGTGCTCATGGTTACAGCA
AGAATAGTGCACGGTTTAAAAGTTAGTCTGGGTGCGTAGGGTCTTGGCTC
CTGGCTGCAAACTCATCGCCGCAGTTCTTCTTCCGCGACTGTACGTCGTT
CGTGCATTGGTGCATTTCTCATAGCCCTTGCGCGTGCTCCGCCCACCGCA
AGACTCTCCGTCCAACTCTAAGCACGCCTGACCGGCCGGCCGCTTTGTGC
TGCCGCATACCCGACCCTTCTGTAGACACCAATGTCTAGGCAACGGTGTT
ACAACAAAATGAGCCAAATTCACGCACAAAGAGTCCATAAAGAAATATCC
CTACTAATGAGACAACTAAACCCGGAGTTACCTCGGGTCAAAAGCATGGC
TGTTTTTGAGTAGGCCCCGTGCCCGCCGACGCCTTCCTTTTCAATGGAAC
TCTTTCGCAGGCAATGTTCGTCCACCTACCGGACGTGGCGGTGATGGGCC
TAGACGATATGGCCGTCATGGTGAGCTTCGTGGCGCAGAGGATCATGCTC
GGCACCGTGGGGCTGCTCCCCGACGCTTGCAGCGACCGATAG
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig14908:1291..3182-

>mRNA_E-fasciculatus_F_contig14908.3492.1 ID=mRNA_E-fasciculatus_F_contig14908.3492.1|Name=mRNA_E-fasciculatus_F_contig14908.3492.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=792bp|location=Sequence derived from alignment at E-fasciculatus_F_contig14908:1291..3182- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
CCCACGAGATGGGACGGCATCATCCACTTGCCCACGAGATGGGACGGCAT
CATCCACTTGGGCTCCCCCTGCATTCTCCCCACCACGGCGCCCGTGTCCT
TCCTCGATCTCCGTGACCTCACGGAACTCCTGCAAGACGGCGGGCACGCC
GACCTCGTGTCTCTGGAGACTTGGAGCAGGGACGCCGGGGCGTACTACTG
CAACGAGATGTTCTACCGCACGCTGCACGCCATTCGCGAGCTGGGCACCC
CGTCGGTAGATGGATCTTCTCTTATCCCGGCGAGTTTTTTTTCCGGCTCC
CCCTGCATTCTCCCCACCACGGCGCCCGTGTCCTTCCTCGATCTCCGTGA
CCTCACGGAACTCCTGCAAGACGGCGGGCACGCCGACCTCGTGTCTCTGG
AGACTTGGAGCAGGGACGCCGGGGCGTACTACTGCAACGAGATGTTCTAC
CGCACGCTGCACGCCATTCGCGAGCTGGGCACCCCGTCGGTAGATGGATC
TTCTCTTATCCCGGCGAGTTTTTTTTCCGCAATGTTCGTCCACCTACCGG
ACGTGGCGGTGATGGGCCTAGACGATATGGCCGTCATGGTGAGCTTCGTG
GCGCAGAGGATCATGCTCGGCACCGTGGGGCTGCTCCCCGACGCTTGCAG
CGACCGATAGGCAATGTTCGTCCACCTACCGGACGTGGCGGTGATGGGCC
TAGACGATATGGCCGTCATGGTGAGCTTCGTGGCGCAGAGGATCATGCTC
GGCACCGTGGGGCTGCTCCCCGACGCTTGCAGCGACCGATAG
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