prot_E_fasciculatus_S2_contig9105.17184.1 (polypeptide) Ectocarpus fasciculatus EfasUO2

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview
NamemRNA_E_fasciculatus_S2_contig9105.17184.1
Unique Nameprot_E_fasciculatus_S2_contig9105.17184.1
Typepolypeptide
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Sequence length234
Homology
BLAST of mRNA_E_fasciculatus_S2_contig9105.17184.1 vs. uniprot
Match: D8LF05_ECTSI (Gamma-glutamyl transpeptidase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LF05_ECTSI)

HSP 1 Score: 442 bits (1136), Expect = 3.810e-151
Identity = 219/236 (92.80%), Postives = 226/236 (95.76%), Query Frame = 0
Query:    1 LYHPSRASADVKKGSPTKSSCTVSFQVVDEQGNAVSFVNSNYLGFGSGIVPDGCGFSLQSRGSNFVLDPEHPNVLAPGKRPYHTIIPCMVTSEATGELFATMTNMGGFMQPQGHVQLLSNLLLRGMDPQAAIDAPRFCIRDGMANGAVALEPWFPDAAA---GRETPEGADKGSGEEGYEAVVSELQRMGHAIKVVRGHARCEMGRAQIIQRKMSGGTQVLWCGSDGRADGCAVAW 233
            L+ P+RAS DVKKGSPTKSSCTVSFQVVD++GNAVSFVNSNY+GFGSGIVPDGCGFSLQSRGSNFVLDPEHPNVLAPGKRPYHTIIPCMVTSEATGELFATMTNMG FMQPQGHVQLLSNLLLRGMDPQAAIDAPRFCIRDGMANGAVALEPW+PDAAA   GR+TPEGADKG   EGYEAVVSELQRMGH IKVVRGHARCEMGRAQIIQRKMSGGTQVLWCGSDGRADGCAVAW
Sbjct:  357 LFDPTRASVDVKKGSPTKSSCTVSFQVVDDRGNAVSFVNSNYMGFGSGIVPDGCGFSLQSRGSNFVLDPEHPNVLAPGKRPYHTIIPCMVTSEATGELFATMTNMGAFMQPQGHVQLLSNLLLRGMDPQAAIDAPRFCIRDGMANGAVALEPWYPDAAAAAAGRDTPEGADKGG--EGYEAVVSELQRMGHDIKVVRGHARCEMGRAQIIQRKMSGGTQVLWCGSDGRADGCAVAW 590          
BLAST of mRNA_E_fasciculatus_S2_contig9105.17184.1 vs. uniprot
Match: W7TJT7_9STRA (Gamma-glutamyltranspeptidase n=2 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TJT7_9STRA)

HSP 1 Score: 254 bits (650), Expect = 2.220e-78
Identity = 139/235 (59.15%), Postives = 164/235 (69.79%), Query Frame = 0
Query:    2 YHPSRASADVKKGSPTKSSCTVSFQVVDEQGNAVSFVNSNYLGFGSGIVPDGCGFSLQSRGSNFVLDPEHPNVLAPGKRPYHTIIPCMVTSEATGELFATMTNMGGFMQPQGHVQLLSNLLLRGMDPQAAIDAPRFCIRDGMANGAVALEPWFPDAAAGRETPEGADKGSGEEGYEAVVSELQRMGHAI--KVVRGHA-RCEMGRAQIIQRKMSGGTQVLWCGSDGRADGCAVAW 233
            +HP+RA ADV+ G+PT+SS TVSFQVVD  GNAVSFVNSNY+ FGSG+VP GCGF+LQ+RG NF L P HPNVLAPGKRP+HTIIP + T   +G L+A+ +NMGGFMQPQGHVQLL NLLL  MDPQAA+DAPRFCI+DG A GA ALE             EG +        E V+ EL+  GH +  +  RG+A R   G+AQ+I R     T VLW GSDGR DGC   W
Sbjct:  349 FHPTRAQADVQAGTPTRSSSTVSFQVVDPAGNAVSFVNSNYMHFGSGLVPRGCGFTLQNRGHNFSLSPTHPNVLAPGKRPFHTIIPGLATRLDSGALYASFSNMGGFMQPQGHVQLLLNLLLHRMDPQAAVDAPRFCIQDGSAQGAAALE-------------EGIE--------EDVLEELRARGHRVAGEERRGYAHRVVFGKAQVIVRDPV--TGVLWGGSDGRGDGCVYGW 560          
BLAST of mRNA_E_fasciculatus_S2_contig9105.17184.1 vs. uniprot
Match: K0SP96_THAOC (Uncharacterized protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0SP96_THAOC)

HSP 1 Score: 253 bits (645), Expect = 7.240e-77
Identity = 136/224 (60.71%), Postives = 155/224 (69.20%), Query Frame = 0
Query:   11 VKKGSPTKSSCTVSFQVVDEQGNAVSFVNSNYLGFGSGIVPDGCGFSLQSRGSNFVLDPEHPNVLAPGKRPYHTIIPCMVTSEATGELFATMTNMGGFMQPQGHVQLLSNLLLRGMDPQAAIDAPRFCIRDGMANGAVALEPWFPDAAAGRETPEGADKGSGEEGYEAVVSELQRMGHAIKV-VRGHARCEMGRAQIIQRKMSGGTQVLWCGSDGRADGCAVAW 233
            V +G+PT SSCTVSFQVVDE+GNA+SFVNSNY+GFG+G+ P GCGF+LQ+RGS F LD  HPN LAP KRPYHTIIPCMVT   TGEL++T+TNMGGFMQPQGH+QL  NLL  GMDPQ AIDAPRFCI DG  NG V +E  F         PE              V  L RMGH +K  V G+ R   GRAQ+I R     T VL  GSDGRADGCA+ +
Sbjct:  439 VVQGTPTPSSCTVSFQVVDERGNAMSFVNSNYMGFGTGLTPKGCGFTLQNRGSGFSLDATHPNALAPNKRPYHTIIPCMVTHSDTGELYSTLTNMGGFMQPQGHLQLAVNLLALGMDPQTAIDAPRFCILDGTRNGTVFVETGF--------LPE-------------TVEALGRMGHRMKSGVAGNEREVFGRAQVITRDRE--TGVLCAGSDGRADGCALGY 639          
BLAST of mRNA_E_fasciculatus_S2_contig9105.17184.1 vs. uniprot
Match: A0A7S2WGH2_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WGH2_9STRA)

HSP 1 Score: 248 bits (632), Expect = 2.100e-75
Identity = 132/227 (58.15%), Postives = 156/227 (68.72%), Query Frame = 0
Query:    7 ASADVKKGSPTKSSCTVSFQVVDEQGNAVSFVNSNYLGFGSGIVPDGCGFSLQSRGSNFVLDPEHPNVLAPGKRPYHTIIPCMVTSEATGELFATMTNMGGFMQPQGHVQLLSNLLLRGMDPQAAIDAPRFCIRDGMANGAVALEPWFPDAAAGRETPEGADKGSGEEGYEAVVSELQRMGHAIKVVRGHARCEMGRAQIIQRKMSGGTQVLWCGSDGRADGCAVAW 233
            A+  ++ G P +SSCTVSFQVVD  GNAVS VNSNY GFG+G  P G GF+LQ+RG NF L+P HPNV+APGKRPYHTIIP M+T  ++G+L A+ +NMGGFMQPQGHVQLL N+   GMDPQAAIDAPRFCI+DG + G V LE             +G D         +VV+ELQR GH+  + +GHAR   GRAQII R     T VL  GSDGR DGCA  W
Sbjct:  387 ANLSLEHGFPERSSCTVSFQVVDRWGNAVSMVNSNYCGFGTGYTPKGYGFTLQNRGHNFSLEPGHPNVVAPGKRPYHTIIPAMLTCSSSGQLLASYSNMGGFMQPQGHVQLLLNMTKAGMDPQAAIDAPRFCIQDGTSKGKVCLE-------------DGIDP--------SVVAELQRRGHSASLEKGHARGVFGRAQIIVRNPD--TGVLVGGSDGRGDGCAAGW 590          
BLAST of mRNA_E_fasciculatus_S2_contig9105.17184.1 vs. uniprot
Match: A0A4D9CY46_9STRA (Uncharacterized protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CY46_9STRA)

HSP 1 Score: 249 bits (636), Expect = 9.780e-74
Identity = 138/232 (59.48%), Postives = 162/232 (69.83%), Query Frame = 0
Query:    2 YHPSRASADVKKGSPTKSSCTVSFQVVDEQGNAVSFVNSNYLGFGSGIVPDGCGFSLQSRGSNFVLDPEHPNVLAPGKRPYHTIIPCMVTSEATGELFATMTNMGGFMQPQGHVQLLSNLLLRGMDPQAAIDAPRFCIRDGMANGAVALEPWFPDAAAGRETPEGADKGSGEEGYEAVVSELQRMGHAI--KVVRGHA-RCEMGRAQIIQRKMSGGTQVLWCGSDGRADGCA 230
            +HP+RA ADV+ G+PT+SS TVSFQVVD  GNAVSFVNSNY+ FGSG+VP GCGFSLQ+RG NF L P HPNVLAPGKRP+HTIIP + T   +G L+A+ +NMGGFMQPQGHVQLL NLLL  MDPQAA+DAPRFCI+DG A GA ALE             EG +        E V+ EL+  GH +  +  RG+  R   G+AQ+I R     T VLW GSDGR DG A
Sbjct:  349 FHPTRAQADVQAGTPTRSSSTVSFQVVDPAGNAVSFVNSNYMHFGSGLVPQGCGFSLQNRGHNFSLSPSHPNVLAPGKRPFHTIIPGLATRLDSGALYASFSNMGGFMQPQGHVQLLLNLLLHRMDPQAAVDAPRFCIQDGSAQGAAALE-------------EGIE--------EDVLEELRARGHRVAGEERRGYVHRVVFGKAQVIVRDPV--TGVLWGGSDGRGDGAA 557          
BLAST of mRNA_E_fasciculatus_S2_contig9105.17184.1 vs. uniprot
Match: A0A7S2ERD2_TRICV (Hypothetical protein n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S2ERD2_TRICV)

HSP 1 Score: 240 bits (613), Expect = 3.430e-73
Identity = 127/233 (54.51%), Postives = 155/233 (66.52%), Query Frame = 0
Query:    2 YHPSRASADVKKGSPTKSSCTVSFQVVDEQGNAVSFVNSNYLGFGSGIVPDGCGFSLQSRGSNFVLDPEHPNVLAPGKRPYHTIIPCMVTSEATGELFATMTNMGGFMQPQGHVQLLSNLLLRGMDPQAAIDAPRFCIRDGMANGAVALEPWFPDAAAGRETPEGADKGSGEEGYEAVVSELQRMGHA-IKVVRGHARCEMGRAQIIQRKMSGGTQVLWCGSDGRADGCAVAW 233
            Y P+RA   V +G+P  +SCTVSFQVVD  G+AVSFVNSN+ GFG+G+VP+GCGFSLQ+RG  F LDP HPN LAP KRP+HTIIP M+T   TGEL+AT++NMGG+MQPQGH+QL   ++  G+DPQ+A+DAPRFCI DG   G V LE    D                      V+  L+  GH  +  V GH R   GRAQII+R     T VLW GSDGRADGCA+ +
Sbjct:  323 YDPNRA---VAQGAPPPTSCTVSFQVVDSDGDAVSFVNSNFKGFGTGLVPEGCGFSLQNRGFGFSLDPAHPNALAPNKRPFHTIIPGMLTHADTGELYATLSNMGGYMQPQGHMQLTVGMVAGGLDPQSAVDAPRFCIADGTIGGTVMLEEGMSDE---------------------VIRGLKDRGHKMVSGVSGHERALFGRAQIIKRDRR--TGVLWAGSDGRADGCAMGY 529          
BLAST of mRNA_E_fasciculatus_S2_contig9105.17184.1 vs. uniprot
Match: A0A835Z955_9STRA (Gamma-glutamyl transpeptidase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z955_9STRA)

HSP 1 Score: 240 bits (612), Expect = 5.150e-72
Identity = 131/243 (53.91%), Postives = 155/243 (63.79%), Query Frame = 0
Query:    1 LYHPSRASADVKKGSPTKSSCTVSFQVVDEQGNAVSFVNSNYLGFGSGIVPDGCGFSLQSRGSNFVLDPEHPNVLAPG----------KRPYHTIIPCMVTSEATGELFATMTNMGGFMQPQGHVQLLSNLLLRGMDPQAAIDAPRFCIRDGMANGAVALEPWFPDAAAGRETPEGADKGSGEEGYEAVVSELQRMGHAIKVVRGHARCEMGRAQIIQRKMSGGTQVLWCGSDGRADGCAVAW 233
            L+ P  A   ++ G P   SCTVSFQVVD+ GNAVS V SNY GFG+G+VP GCGF+LQ+RG NF LD  HPN LAP            RPYHTIIPCM T  ATGEL+AT+TNMG FMQPQGH+QLL +LL RG+DPQ A+D PRFCI D      V +E   PD                      VV  L+R GH ++V+ GH+R + GRAQII R  + G  VLWCGSDGRADG A+A+
Sbjct:  419 LFDPQSACRQLQHGHPAAQSCTVSFQVVDDAGNAVSMVVSNYEGFGTGLVPTGCGFTLQNRGLNFSLDAAHPNCLAPSAHPLPFSLMSNRPYHTIIPCMATVAATGELYATLTNMGRFMQPQGHLQLLCHLL-RGVDPQTAVDMPRFCITDIDGGRRVDIEDGTPDE---------------------VVESLRRRGHDVRVLSGHSRAQFGRAQIILRDGTSG--VLWCGSDGRADGMAIAF 637          
BLAST of mRNA_E_fasciculatus_S2_contig9105.17184.1 vs. uniprot
Match: L8H169_ACACA (Gammaglutamyltranspeptidase n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8H169_ACACA)

HSP 1 Score: 237 bits (604), Expect = 8.230e-72
Identity = 125/233 (53.65%), Postives = 152/233 (65.24%), Query Frame = 0
Query:    2 YHPSRASADVKKGSPTKSSCTVSFQVVDEQGNAVSFVNSNYLGFGSGIVPDGCGFSLQSRGSNFVLDPEHPNVLAPGKRPYHTIIPCMVTSEATG--ELFATMTNMGGFMQPQGHVQLLSNLLLRGMDPQAAIDAPRFCIRDGMANGAVALEPWFPDAAAGRETPEGADKGSGEEGYEAVVSELQRMGHAIKVVRGHARCEMGRAQIIQRKMSGGTQVLWCGSDGRADGCAVA 232
            + P +A+ DV+KGSP  SSCTV F VVDEQGNA SF+NSNY+GFGSGI+P GCGF+LQ+RG+NF L+   PN+L   KRPYHTIIP M T +  G  EL+ +   MGGFMQPQGHVQ+L N++   M+PQ A+DAPRFCI DG +NG V++E   P                     EAVV +L  MGH + +VR H R   GR QII R     T V W GSD RADG A+A
Sbjct:  322 FKPDKAAVDVEKGSPASSSCTVYFSVVDEQGNACSFINSNYVGFGSGIIPKGCGFTLQNRGANFTLEEGSPNMLEGNKRPYHTIIPGMATHQVNGKVELYCSFGVMGGFMQPQGHVQVLLNMIDYKMNPQQAVDAPRFCIEDGTSNGTVSIEEGVP---------------------EAVVQQLADMGHRVVIVRDHDRATFGRGQIIWRHPV--TGVFWAGSDPRADGMAIA 531          
BLAST of mRNA_E_fasciculatus_S2_contig9105.17184.1 vs. uniprot
Match: A0A7S2LJ05_9STRA (Hypothetical protein n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A7S2LJ05_9STRA)

HSP 1 Score: 239 bits (610), Expect = 1.060e-71
Identity = 125/224 (55.80%), Postives = 152/224 (67.86%), Query Frame = 0
Query:   11 VKKGSPTKSSCTVSFQVVDEQGNAVSFVNSNYLGFGSGIVPDGCGFSLQSRGSNFVLDPEHPNVLAPGKRPYHTIIPCMVTSEATGELFATMTNMGGFMQPQGHVQLLSNLLLRGMDPQAAIDAPRFCIRDGMANGAVALEPWFPDAAAGRETPEGADKGSGEEGYEAVVSELQRMGHAIKV-VRGHARCEMGRAQIIQRKMSGGTQVLWCGSDGRADGCAVAW 233
            V +G PT SSCTVSFQVVDE GNA+SFVNSNY+GFGSG+ P  CGF+LQ+RG+ F LD  HPN LAP KRPYHTIIP M+T   TGEL+A+++NMGGFMQPQGH+QL+  L+  G+DPQ A+DAPRFCI DG  NG + +E  F                      EA V++L R GH ++  V GH R   GRAQII +     T +L  GSDGRADGCA+ +
Sbjct:  439 VVQGKPTPSSCTVSFQVVDELGNAMSFVNSNYMGFGSGLTPTNCGFTLQNRGAGFSLDTNHPNALAPRKRPYHTIIPAMLTHSDTGELYASLSNMGGFMQPQGHMQLVVQLVANGLDPQTAVDAPRFCILDGSQNGKIFIETGF---------------------NEATVAKLARYGHNMEPNVSGHEREIFGRAQIITKDRE--TGILAAGSDGRADGCALGY 639          
BLAST of mRNA_E_fasciculatus_S2_contig9105.17184.1 vs. uniprot
Match: A0A1E7FUA8_9STRA (Gamma-glutamyltransferase n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7FUA8_9STRA)

HSP 1 Score: 238 bits (606), Expect = 1.520e-71
Identity = 126/234 (53.85%), Postives = 157/234 (67.09%), Query Frame = 0
Query:    1 LYHPSRASADVKKGSPTKSSCTVSFQVVDEQGNAVSFVNSNYLGFGSGIVPDGCGFSLQSRGSNFVLDPEHPNVLAPGKRPYHTIIPCMVTSEATGELFATMTNMGGFMQPQGHVQLLSNLLLRGMDPQAAIDAPRFCIRDGMANGAVALEPWFPDAAAGRETPEGADKGSGEEGYEAVVSELQRMGHAIKV-VRGHARCEMGRAQIIQRKMSGGTQVLWCGSDGRADGCAVAW 233
            L++P +A+     G P  SSCTVSFQVVD+ GNA+SFVNSNY+GFG+G+VP  CGF+LQ+RG  F L+ +HPNV+AP KRP HTIIP M+T   T EL+AT++NMGG MQPQGH+Q + N++  GMDPQA+ID PRFCI DG  NG V LE                         E+VV EL+R GH +   + GH+R   GRAQII+R    G  VLW GSDGRADGCA+ +
Sbjct:  384 LFNPEKATI---AGMPDPSSCTVSFQVVDKAGNAISFVNSNYMGFGTGMVPKNCGFTLQNRGFGFNLEKDHPNVIAPRKRPCHTIIPGMITHRETNELYATISNMGGNMQPQGHMQHVVNMITGGMDPQASIDFPRFCIPDGTQNGIVQLENGI---------------------NESVVKELKRRGHTLMSGIVGHSRAIFGRAQIIKRNRDNG--VLWAGSDGRADGCAIGY 591          
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig9105.17184.1 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LF05_ECTSI3.810e-15192.80Gamma-glutamyl transpeptidase n=2 Tax=Ectocarpus T... [more]
W7TJT7_9STRA2.220e-7859.15Gamma-glutamyltranspeptidase n=2 Tax=Nannochlorops... [more]
K0SP96_THAOC7.240e-7760.71Uncharacterized protein n=1 Tax=Thalassiosira ocea... [more]
A0A7S2WGH2_9STRA2.100e-7558.15Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A4D9CY46_9STRA9.780e-7459.48Uncharacterized protein n=1 Tax=Nannochloropsis sa... [more]
A0A7S2ERD2_TRICV3.430e-7354.51Hypothetical protein n=1 Tax=Trieres chinensis Tax... [more]
A0A835Z955_9STRA5.150e-7253.91Gamma-glutamyl transpeptidase n=1 Tax=Tribonema mi... [more]
L8H169_ACACA8.230e-7253.65Gammaglutamyltranspeptidase n=1 Tax=Acanthamoeba c... [more]
A0A7S2LJ05_9STRA1.060e-7155.80Hypothetical protein n=1 Tax=Skeletonema marinoi T... [more]
A0A1E7FUA8_9STRA1.520e-7153.85Gamma-glutamyltransferase n=1 Tax=Fragilariopsis c... [more]

Pages

back to top
InterPro
Analysis Name: InterProScan on OGS1.0 of Ectocarpus fasciculatus EfasUO2
Date Performed: 2022-09-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR01210GGTRANSPTASEcoord: 22..40
score: 37.56
coord: 77..92
score: 36.93
NoneNo IPR availablePFAMPF01019G_glu_transpeptcoord: 11..228
e-value: 3.3E-43
score: 148.2
NoneNo IPR availableGENE3D3.60.20.40coord: 22..233
e-value: 7.4E-64
score: 216.8
NoneNo IPR availablePANTHERPTHR43881:SF1coord: 2..233
NoneNo IPR availablePANTHERPTHR43881FAMILY NOT NAMEDcoord: 2..233
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 17..233

Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig9105contigE_fasciculatus_S2_contig9105:193..2505 +
Analyses
This polypeptide is derived from or has results from the following analyses
Analysis NameDate Performed
InterProScan on OGS1.0 of Ectocarpus fasciculatus EfasUO22022-09-29
Diamond blastp: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-16
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Relationships

This polypeptide derives from the following mRNA feature(s):

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig9105.17184.1mRNA_E_fasciculatus_S2_contig9105.17184.1Ectocarpus fasciculatus EfasUO2mRNAE_fasciculatus_S2_contig9105 193..2505 +


Sequences
The following sequences are available for this feature:

polypeptide sequence

>prot_E_fasciculatus_S2_contig9105.17184.1 ID=prot_E_fasciculatus_S2_contig9105.17184.1|Name=mRNA_E_fasciculatus_S2_contig9105.17184.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=234bp
LYHPSRASADVKKGSPTKSSCTVSFQVVDEQGNAVSFVNSNYLGFGSGIV
PDGCGFSLQSRGSNFVLDPEHPNVLAPGKRPYHTIIPCMVTSEATGELFA
TMTNMGGFMQPQGHVQLLSNLLLRGMDPQAAIDAPRFCIRDGMANGAVAL
EPWFPDAAAGRETPEGADKGSGEEGYEAVVSELQRMGHAIKVVRGHARCE
MGRAQIIQRKMSGGTQVLWCGSDGRADGCAVAW*
back to top
Annotated Terms
The following terms have been associated with this polypeptide:
Vocabulary: INTERPRO
TermDefinition
IPR029055Ntn_hydrolases_N