mRNA_E_fasciculatus_S2_contig9219.17279.1 (mRNA) Ectocarpus fasciculatus EfasUO2

BLAST of mRNA_E_fasciculatus_S2_contig9219.17279.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K396_9PHAE)

HSP 1 Score: 617 bits (1591), Expect = 2.630e-219
Identity = 304/337 (90.21%), Postives = 317/337 (94.07%), Query Frame = 1

Query:    4 AFDIDGVLVRGEGVLPGARESLKALEDARIPYVFVTNGGGCTEEAKARDLTSKLGVQVHRSMVVLSHSPMRALAPEYSGKRVMVLGSSAKKIAEEDLGLLDTVTPEEFAGEWPDIFHFTGDGYYIPRNFPRRLAEGERPRSVSAVMVLHDPLDWALEAQVVVDVLRGGEPPGSGGASQTPMYISNPDFVFASMYQEPRFAAGAFADTVRMLYAKRFATGEPTIEVFGKPTISTFDYAKRLLEERHGVIRLQQQHKATSKIERIYMVGDNPSGDIRGANAAGPPWISNLVKTGVYRDTGDRGNDAEDPAHFVHDGVRQCVDFAMERETLGVDEYRGDE 1014
            AFDIDGVLVRG+ VLPGARESLKALEDAR+PYVFVTNGGGCTEEAKARDLTSKLGVQVHRSMV LSHSPMRALA EYSGKRVMVLGSSAKKIAEEDLGLLDTVTPEEFAGEWPDIFHFTGD YYIPRNFPRRLAEGERPRSVSAVMVLHDPLDWALEAQVVVDVLRGG+PPGSGGASQTPMYISNPDFVFAS YQEPRFAAGAFADTVRMLYAKRFAT EPTIEVFGKPTISTFDYA+RLLEER GVIR Q++HK    IERIYMVGDNPSGDIRGANAAGPPW+SNLVKTGV+RD G RGNDAEDPAHFVH+ VR+CV+FAMERE   V+EY  D+
Sbjct:   33 AFDIDGVLVRGQEVLPGARESLKALEDARVPYVFVTNGGGCTEEAKARDLTSKLGVQVHRSMVALSHSPMRALASEYSGKRVMVLGSSAKKIAEEDLGLLDTVTPEEFAGEWPDIFHFTGDRYYIPRNFPRRLAEGERPRSVSAVMVLHDPLDWALEAQVVVDVLRGGDPPGSGGASQTPMYISNPDFVFASTYQEPRFAAGAFADTVRMLYAKRFATSEPTIEVFGKPTISTFDYARRLLEERDGVIRPQEEHK----IERIYMVGDNPSGDIRGANAAGPPWLSNLVKTGVFRDKGGRGNDAEDPAHFVHNDVRECVNFAMEREARRVEEYPCDQ 365          

BLAST of mRNA_E_fasciculatus_S2_contig9219.17279.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FVJ2_ECTSI)

HSP 1 Score: 346 bits (887), Expect = 3.340e-115
Identity = 168/188 (89.36%), Postives = 178/188 (94.68%), Query Frame = 1

Query:  439 MVLHDPLDWALEAQVVVDVLRGGEPPGSGGASQTPMYISNPDFVFASMYQEPRFAAGAFADTVRMLYAKRFATGEPTIEVFGKPTISTFDYAKRLLEERHGVIRLQQQHKATSKIERIYMVGDNPSGDIRGANAAGPPWISNLVKTGVYRDTGDRGNDAEDPAHFVHDGVRQCVDFAMERETLGVDEY 1002
            MVLHDPLDWALEAQVVVDVLRGG+PPGSGGASQTPMYISNPDFVFASMYQEPRFAAGAFADTVRMLYAKRFAT EPTIEVFGKPTISTFDYA+RLLEER GVIR QQ   ATSKIERIYMVGDNPSGDIRGANAAGPPW+SNLVKTGV+RDTG+RGNDA+DPAHFVHD V++CV+FA+ERE  G DEY
Sbjct:    1 MVLHDPLDWALEAQVVVDVLRGGDPPGSGGASQTPMYISNPDFVFASMYQEPRFAAGAFADTVRMLYAKRFATSEPTIEVFGKPTISTFDYARRLLEERDGVIRPQQ---ATSKIERIYMVGDNPSGDIRGANAAGPPWVSNLVKTGVFRDTGNRGNDAKDPAHFVHDDVKECVNFAIEREARGGDEY 185          

BLAST of mRNA_E_fasciculatus_S2_contig9219.17279.1 vs.
Match: HAD-like (domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YU48_9STRA)

HSP 1 Score: 331 bits (848), Expect = 3.420e-106
Identity = 188/404 (46.53%), Postives = 232/404 (57.43%), Query Frame = 1

Query:    1 QAFDIDGVLVRGEGVL------------------------------------PGARESLKALEDARIPYVFVTNGGGCTEEAKARDLTSKLGVQVHRSMVVLSHSPMRALAPEYSGKRVMVLGSSAKKIAEEDLGLLDTVTPEEFAGEWPDIFHFTGDGYYIPRNF--PRRLAEGERPRSVSAVMVLHDPLDWALEAQVVVDVLRGGEPPGSGGAS--QTPMYISNPDFVFASMYQEPRFAAGAFADTVRMLYAKRFATGEPTIEV-------------------------------------FGKPTISTFDYAKRLLEERHGVIRLQQQHKATSKIERIYMVGDNPSGDIRGANAAGPPWISNLVKTGVYRDTGDRGNDAEDPAHFVHDGVRQCVDFAMERE 981
            QAF IDGVL RG+  +                                      A E+L+ LE  R+P +F+TNGGGCTE  KA +L  K G++V R MV+LSH+PMR LA  Y+ +RV+VLGS+  +IA +DLGL +  T EE A EWP ++ F G  YYIPRNF  P   +  +    V AVMVLHDPLDWALEAQVV DVLRGG PPG+G A   QTP +ISNPDF FA+ Y  PRFAAGAFAD V++LY KRF       E                                      +GKPT++TF YA+R+L+ R   + L       +   RIYMVGDNP+GDIRGANAAG  W+SNLV+TG++  TG   ND +DPA  VHD  RQCVDF ++RE
Sbjct:    6 QAFGIDGVLARGKDEIIHASNTFCQWNNSFHTHAXXXXXXXXXXXXXXXXXXXXAAETLRKLEQLRVPLIFLTNGGGCTEAQKAAELAKKFGIKVTRDMVLLSHTPMRDLAAAYARRRVLVLGSACTQIARKDLGLQNVTTVEEVAREWPTLYPFQGPKYYIPRNFSLPPGASAPKPGAPVEAVMVLHDPLDWALEAQVVTDVLRGGSPPGAGRADGGQTPWFISNPDFTFAAQYPHPRFAAGAFADVVKLLYKKRFGADAKVTETASAILMRGCRAARAPCTHALRCRLRFRRCLRSRCRRQYGKPTLATFRYAERMLDARAKAMGL------AAGPHRIYMVGDNPAGDIRGANAAGERWVSNLVRTGLF--TGAH-NDPQDPADHVHDDARQCVDFVLQRE 400          

BLAST of mRNA_E_fasciculatus_S2_contig9219.17279.1 vs.
Match: Uncharacterized (protein n=3 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A0G4F8D8_VITBC)

HSP 1 Score: 244 bits (624), Expect = 2.800e-73
Identity = 139/328 (42.38%), Postives = 187/328 (57.01%), Query Frame = 1

Query:    7 FDIDGVLVRGEGVLPGARESLKALEDARIPYVFVTNGGGCTEEAKARDLTSKLGVQVHRSMVVLSHSPMRALAPEYSGKRVMVLGSSAKKIAEEDLGLLDTVTPEEFAGEWPDIFHFTGDGYYIPRNFPRRLAEGERPRSV--SAVMVLHDPLDWALEAQVVVDVLRGGEPPGSGGASQTP-MYISNPDFVFASMYQEPRFAAGAFADTVRMLYAKRFATGEPTIEVFGKPTISTFDYAKRLLEERHGVIR-LQQQHKATSKIERIYMVGDNPSGDIRGANAAGPPWISNLVKTGVYRDTGDRGNDAEDPAHFVHDGVRQCVDFAMER 978
            FDIDGVLVRG+  L GA+E+LK L D  +P+ F+TNGGG TE  KA +LT+ L  +V    V +SH+PMR +A  Y+ +RVMV+GS       +  G  D +TP++F  EWP+++             P R  E  RP+     AV++LHDP DWALEAQV+ D+L G     + G   +P +Y SNPDF++++ +  PRF  GAF   +  L+ K      P +  +GKP    + YA R+LE     IR L      T   +RIY +GDNP  D+RGAN AG PW S LV+TGV+       ND +DPAH V   V   V   +ER
Sbjct:   43 FDIDGVLVRGKKALQGAKEALKLLRDGDLPFTFLTNGGGITESNKAEELTTLLDTEVSPDQVQMSHTPMREVAQRYTDRRVMVVGSKRFLDVAKSCGFSDIITPQDFLQEWPNLY-------------PYRKVE-TRPKGPLPEAVVILHDPQDWALEAQVLCDILSGSL---AAGEEHSPHLYASNPDFLYSAAHHTPRFGQGAFTTALEHLFEKLHGRPLP-VTYYGKPNAVVYRYATRILEAEVRRIRSLPAADNVT--FDRIYAIGDNPKADVRGANRAGLPWTSVLVRTGVFS---GEDNDPDDPAHIVSHDVHAAVKMIVER 347          

BLAST of mRNA_E_fasciculatus_S2_contig9219.17279.1 vs.
Match: Hypothetical (protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813CRI9_9DINO)

HSP 1 Score: 242 bits (617), Expect = 5.200e-72
Identity = 148/345 (42.90%), Postives = 186/345 (53.91%), Query Frame = 1

Query:    4 AFDIDGVLVRGEGVLPGARESLKALEDARIPYVFVTNGGGCTEEAKARDLTSKLGVQVHRSMVVLSHSPMRALAPEYSGKRVMVLGSSAKKIAEEDLGLLDTVTPEEFAGEWPDIFHFTGDGYYIPRNFPRRLAEGERPRSVSAVMVLHDPLDWALEAQVVVDVLRGGEPPGSGGASQTPMYISNPDFVFASMYQEPRFAAGAFADTVRMLYAKRFATGEPTIEVFGKPTISTFDYAKRLLEERHGVIRLQQQHKATSKIERIYMVGDNPSGDIRGANAAGPPWISNLVKTGVY---------------------RDTGD-----RGNDAEDPAHFVHDGVRQCV 960
            AFDIDGVL+RGEGVL GA E+L+ L    IP+VF+TNGGG  E AKA  L   LGV V    V++SHSPMR LA +Y+G++V+VLG    K   E  G    VT +  A   P  + F     +  +  P  +A+        AV+++HDP+ WA + Q+ +DVLRGG+P GSG     P+Y SNPDFVFA  +  PRFA GAF  T+  L+ K+    +  I  +GKP   TFD+A   L+ + G   LQ          R+YMVGDNP  DIRGAN AG PW S LV TGV+                     R T        GND  DPA  V  GV   V
Sbjct:   36 AFDIDGVLLRGEGVLTGAPEALQLLHACGIPHVFLTNGGGVLESAKAAQLADALGVPVAPEQVIVSHSPMRPLAEKYAGQKVLVLGWRDVKGVAEAYGFAPVVTVDCLASHDPTAYPFMS---WPDKRLPPSVADAP----FGAVLIMHDPVHWAPDLQLALDVLRGGDPLGSGTGQAVPLYASNPDFVFAGRHPVPRFAQGAFTQTLSFLF-KQLTGQDLAITQYGKPHAVTFDFALERLQAQAGGPPLQ----------RVYMVGDNPKADIRGANWAGDPWRSLLVSTGVHGAPREAFTSPALQGGSHQELLRSTPSLAADGTGNDVTDPASAVVAGVLDAV 362          

BLAST of mRNA_E_fasciculatus_S2_contig9219.17279.1 vs.
Match: Uncharacterized (protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CC39_PYTOL)

HSP 1 Score: 237 bits (605), Expect = 8.610e-71
Identity = 139/333 (41.74%), Postives = 192/333 (57.66%), Query Frame = 1

Query:    4 AFDIDGVLVRGEGVLPGARESLKALEDARIPYVFVTNGGGCTEEAKARDLTSKLGVQVHRSMVVLSHSPMRALAPEYSGKRVMVLGSSAKKIAEEDLGLLDTVTPEEFAGEWPDIFHFTGDGYYIPRNFPRRLAEGERPRSVSAVMVLHDPLDWALEAQVVVDVLRGGEPPGSG--GASQTPMYISNPDFVFASMYQEPRFAAGAFADTVRMLYAKRFATGEPTIEVFGKPTISTFDYAKRLLEERHGVIRLQQQHKATSKIERIYMVGDNPSGDIRGANAAGPPWISNLVKTGVYRDTGDRGNDAEDPAHFVH---DGVRQCVDFAMERETL 987
            AFDIDGVLVRG  V+P ARE LK+L++  IP+VF+TN GG  E AKA  L   LGV++    ++LSH+PMR LA ++   R+++LGSS       + G     T E+ A   P  F F     Y  +N P      + P  + A++++HDP+DWA E Q+ VDVL GG P GSG    +QTP++ISN DFVF+S Y  PRFA GAF   +R+LY K +   +  +  +GKP   T+ YA+ LL +  G         A++ ++ IY +GDNP  DI+GAN AG  W S LV+TG+Y  T       +DP H      D V + + +  + E L
Sbjct:   20 AFDIDGVLVRGGHVIPRAREVLKSLQEKNIPHVFLTNNGGVLEAAKAGSLGKTLGVEIEPERMILSHTPMRGLAEKFRDSRILLLGSSDVNNVAREYGFKKITTVEDLARHSPGQFAFKT---YEQKNAPYH----DEP--IEAIIIMHDPIDWAPELQITVDVLIGGNPLGSGRPDGTQTPLFISNEDFVFSSEYPFPRFAQGAFMSCLRLLYNK-YTGKDLEVTHYGKPHAVTYAYAEALLNKIAG-------RTASNPLKSIYGIGDNPHADIQGANNAGDHWSSILVRTGIYDGT-------KDPEHIPDVYADTVFEAIQYIYKNEGL 328          

BLAST of mRNA_E_fasciculatus_S2_contig9219.17279.1 vs.
Match: Predicted (phosphatase n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1B333_PLAHL)

HSP 1 Score: 236 bits (603), Expect = 2.750e-70
Identity = 133/335 (39.70%), Postives = 195/335 (58.21%), Query Frame = 1

Query:    4 AFDIDGVLVRGEGVLPGARESLKALEDARIPYVFVTNGGGCTEEAKARDLTSKLGVQVHRSMVVLSHSPMRALAPEYSGKRVMVLGSSAKKIAEEDLGLLDTVTPEEFAGEWPDIFHFTGDGYYIPRNFPRRLAEGERPRSVSAVMVLHDPLDWALEAQVVVDVLRGGEPPGSGGAS--QTPMYISNPDFVFASMYQEPRFAAGAFADTVRMLYAKRFATGEPTIEVFGKPTISTFDYAKRLLEERHGVIRLQQQHKATSKIERIYMVGDNPSGDIRGANAAGPPWISNLVKTGVYRDTGDRGNDAEDPAHFVHDGVRQCVDFAMERETLGVDEY 1002
            AFDIDGVL+RG   LP A+  L++L    + ++F+TNGGGC EE+KA  L++ LG+ ++   ++LSH+PMR L   Y  KRV+++GS       +  G    V+ ++     P  + F         ++ +RLA       + A++V+HDP++WA E QV VDVL GG+PPGSG  S  QTP+++SN DFVF+  YQ PRFA GAF   +++LY + F   +  +  FGKP   T++YA+RLL    G          T  ++ IY +GDNPS DI+GAN  G  W S LV+TGVY    D  N+ +     + D V + +    +RE  G+++Y
Sbjct:   36 AFDIDGVLIRGGHELPNAKRVLQSLRANNVQHIFLTNGGGCMEESKAEKLSNILGLSINPEHMILSHTPMRELTKTYGDKRVLIMGSHDVWHVAKCYGFKKVVSVQDLLHHCPAQYPFN--------HYEQRLAPYYN-EPIEAIIVMHDPINWAPEIQVAVDVLIGGDPPGSGSLSGKQTPLFVSNDDFVFSGEYQSPRFAQGAFTKCLKLLY-EDFTGCKLEMTHFGKPHTITYNYAERLLNSISG---------QTEPLKHIYGIGDNPSSDIQGANNVGERWTSVLVRTGVY----DGVNEPDHTPDVIVDNVYEAIKHIYKRE--GINDY 345          

BLAST of mRNA_E_fasciculatus_S2_contig9219.17279.1 vs.
Match: Uncharacterized (protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WMD0_9STRA)

HSP 1 Score: 234 bits (596), Expect = 1.060e-69
Identity = 127/327 (38.84%), Postives = 188/327 (57.49%), Query Frame = 1

Query:    4 AFDIDGVLVRGEGVLPGARESLKALEDARIPYVFVTNGGGCTEEAKARDLTSKLGVQVHRSMVVLSHSPMRALAPEYSGKRVMVLGSSAKKIAEEDLGLLDTVTPEEFAGEWPDIFHFTGDGYYIPRNFPRRLAEGERPRSVSAVMVLHDPLDWALEAQVVVDVLRGGEPPGSGGASQTPMYISNPDFVFASMYQEPRFAAGAFADTVRMLYAKRFATGEPT-IEVFGKPTISTFDYAKRLLEERHGVIRLQQQHKATSKIERIYMVGDNPSGDIRGANAAGPPWISNLVKTGVYRDTGDRGNDAEDPAHFVHDGVRQCVDFAMERE 981
            AFDIDGVL+RG   LP A ++++ L++  +P++F+TNGGGC EE K + L+  L V +    ++LSH+PMR +A  +   +V+V+G+          G    VT E+ A   P  + F    + +P  FP    E        A++VLHDP+ WA + Q+ VDVL GGEP GSG   QTP+++SN DF F+  Y  PRFA GAF   +++LY +   TG+P  ++ FGKP   T+ +A+ ++           Q ++   IER Y +GDNP  DI+GAN AG  W S LV+TG++    D  N  E PA+ V   V++ V++ +  E
Sbjct:    7 AFDIDGVLIRGGQALPHAAKTIRRLQERCVPHIFLTNGGGCREEKKTKTLSDILQVPLQHEQMILSHTPMRDVAKTFGDSKVLVMGAYEVVDVARHYGFKKVVTVEDLARHSPTQYPFLRWDH-VPSPFPNEAVE--------AIVVLHDPIHWAQDLQIAVDVLVGGEPLGSGQGKQTPLFVSNDDFTFSGAYPVPRFAQGAFTRCLKLLYEQH--TGKPLEVQRFGKPHAVTYKFAEDVM-----------QRQSQGPIERFYGIGDNPFSDIQGANTAGHHWTSILVRTGIFNAPDD--NHDEHPANVVVHSVQEAVEWILNEE 309          

BLAST of mRNA_E_fasciculatus_S2_contig9219.17279.1 vs.
Match: TIGR01456 (family HAD hydrolase n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024TM95_9STRA)

HSP 1 Score: 232 bits (592), Expect = 7.360e-69
Identity = 137/338 (40.53%), Postives = 190/338 (56.21%), Query Frame = 1

Query:    4 AFDIDGVLVRGEGVLPGARESLKALEDARIPYVFVTNGGGCTEEAKARDLTSKLGVQVHRSMVVLSHSPMRALAPEYSGKRVMVLGSSAKKIAEEDLGLLDTVTPEEFAGEWPDIFHFTGDGYYIPRNFPRRLAEGERPRSVSAVMVLHDPLDWALEAQVVVDVLRGGEPPGSG---GASQTPMYISNPDFVFASMYQEPRFAAGAFADTVRMLYAKRFATGEPT-IEVFGKPTISTFDYAKRLLEERHGV-------IRLQQQHKATSKIERIYMVGDNPSGDIRGANAAGPPWISNLVKTGVYRDTGDRGNDAEDPAHFVHDGVRQCVDFAMERET 984
            AFDIDGVL+RG   LP A + ++ L+  ++P++F+TNGGGC EE K   L+  LGV +    ++LSH+PMR LA ++   +V+VLG+       +  G    V+ ++ A   P  + F       P  FP      + P  + A++VLHDP+ WA + Q+ VDVL GG+P GSG   G  QTP+++SN DF F+  Y  PRFA GAF   +++LY +   TG+P  ++ FGKP   T  Y   LL  R GV            Q +A   +ER Y +GDNP  DI+GAN AG  W S LV+TG++ DT    N AE PA  V D V   V + +  ET
Sbjct:    7 AFDIDGVLIRGGKALPQAAKIIRELQQRQVPHIFLTNGGGCREEKKTNTLSQILGVPLRHEQMILSHTPMRDLAKKFGDSKVLVLGAYEVMDVAQHYGFKKVVSVQDLARHSPAQYPFL-KWDPTPSPFP------DDP--IEAIVVLHDPIHWAQDLQIAVDVLVGGDPLGSGRPDGGKQTPLFVSNDDFTFSGAYPVPRFAQGAFTRCLQLLYEQH--TGKPLEVQRFGKPHAVT--YRSVLLSNRSGVHAGSNRFAEDVMQAQAAGPVERYYGIGDNPYSDIQGANNAGDHWTSVLVRTGIFTDT----NHAEHPADVVLDAVDDAVQWILANET 327          

BLAST of mRNA_E_fasciculatus_S2_contig9219.17279.1 vs.
Match: Uncharacterized (protein n=3 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A3F2RHX1_9STRA)

HSP 1 Score: 231 bits (590), Expect = 2.340e-68
Identity = 136/337 (40.36%), Postives = 196/337 (58.16%), Query Frame = 1

Query:    4 AFDIDGVLVRGEGVLPGARESLKALEDARIPYVFVTNGGGCTEEAKARDLTSKLGVQVHRSMVVLSHSPMRALAPEYSGKRVMVLGSSAKKIAEEDLGLLDTVTPEEFAGEWPDIFHFTGDGYYIPRNFPRRLA-EGERPRSVSAVMVLHDPLDWALEAQVVVDVLRGGEPPGSGGAS--QTPMYISNPDFVFASMYQEPRFAAGAFADTVRMLYAKRFATGEPTIEV--FGKPTISTFDYAKRLLEERHGVIRLQQQHKATSKIERIYMVGDNPSGDIRGANAAGPPWISNLVKTGVYRDTGDRGNDAEDPAHFVHDGVRQCVDFAMERETLGVDE 999
            AFDIDGVL+RG   LP AR  L++L    +P++F+TNGGGC EE KA++L+  LGV ++   ++LSH+PMR ++  Y   RV+++GS       ++ G    V+ ++     P  + F          + ++LA   E P  + A++V+HDP+DWA E QV VDVL GG+PPGSG  S  QTP+++SN DFVF+  Y  PRFA GAF   +++LY     TG  T+EV  +GKP   T++YA+ LL    G             ++ IY +GDNP  DI+GAN AG  W S LV++G+Y    D  N+ E       DGV + +    +RE  G+D+
Sbjct:   36 AFDIDGVLIRGGHELPKARRVLESLRANGVPHIFLTNGGGCMEEDKAQNLSDVLGVPINPEHMILSHTPMREISKTYGDSRVLIMGSRDVWNVAKNYGFKKVVSVKDLVRHNPTEYPFL--------RYEQKLAPHHEEP--IEAIIVMHDPIDWAPEIQVAVDVLIGGDPPGSGNPSGKQTPLFVSNDDFVFSGAYPFPRFAQGAFTRCLKLLYED--FTGR-TLEVTNYGKPHNVTYNYAEGLLNTISG---------RAGPLQHIYGIGDNPMSDIQGANNAGDGWTSVLVRSGIY----DGSNEPEHAPDVTVDGVYEAIKHIYKRE--GIDD 344