mRNA_E_fasciculatus_S2_contig9018.17113.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig9018.17113.1
Unique NamemRNA_E_fasciculatus_S2_contig9018.17113.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig9018.17113.1 vs.
Match: Putative (respiratory burst oxidase protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G8C8_ECTSI)

HSP 1 Score: 277 bits (709), Expect = 3.130e-79
Identity = 145/170 (85.29%), Postives = 148/170 (87.06%), Query Frame = 1 
Query:  136 RRQPSAGQLQRARGYHPVSLNFGRPDIEAFITGTVRGTAVVAQWAPPVESLPXXXXXXXXXQQTTASGLRQMLTRLNTGQRISKRDLMEADLTVVDSSLTKSKKPSSVSGVTGFDMPNDSATASKGSGGCVNEKSWLVLYCGANGKVEQAVQTASEGLNVTWRKEYFSKW 645
            R QPSAGQLQRARGYHPVSLNFGRPDI AFITGTVRGTAVVAQWAPPV+S P         QQ TA GLRQMLTRLNTGQRISKRDLMEADLTVVDSSL KSKKPSSVSGVTGFDMPN S   + GS GCV+EKSWLVLYCGANGKVEQAVQTASEGLNV WRKEYFSKW
Sbjct:  754 RGQPSAGQLQRARGYHPVSLNFGRPDIAAFITGTVRGTAVVAQWAPPVDSRPPAPSSSSPRQQPTAGGLRQMLTRLNTGQRISKRDLMEADLTVVDSSLAKSKKPSSVSGVTGFDMPN-SDIPNNGSVGCVDEKSWLVLYCGANGKVEQAVQTASEGLNVAWRKEYFSKW 922
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BLAST of mRNA_E_fasciculatus_S2_contig9018.17113.1 vs.
Match: FAD-binding (FR-type domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JHI2_9PHAE)

HSP 1 Score: 272 bits (695), Expect = 3.730e-77
Identity = 151/168 (89.88%), Postives = 155/168 (92.26%), Query Frame = 1 
Query:  142 QPSAGQLQRARGYHPVSLNFGRPDIEAFITGTVRGTAVVAQWAPPVESLPXXXXXXXXXQQTTASGLRQMLTRLNTGQRISKRDLMEADLTVVDSSLTKSKKPSSVSGVTGFDMPNDSATASKGSGGCVNEKSWLVLYCGANGKVEQAVQTASEGLNVTWRKEYFSKW 645
            QPSAGQLQRARGYHPVSLNFGRPDI AFITGTVRGTAVVAQWAPPVES PXXXXXXXXX   TA GLRQMLTRLNTGQRISKRDLMEADLTVV+ SL KSKKPSS SGV+GFDMPN S TA+ GS GCV+EKSWLVLYCGANGKVEQAVQTASEGLNVTWRKEYFSKW
Sbjct:  771 QPSAGQLQRARGYHPVSLNFGRPDIAAFITGTVRGTAVVAQWAPPVESRPXXXXXXXXXXXPTAGGLRQMLTRLNTGQRISKRDLMEADLTVVNCSLAKSKKPSSTSGVSGFDMPN-SDTANNGSVGCVDEKSWLVLYCGANGKVEQAVQTASEGLNVTWRKEYFSKW 937
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BLAST of mRNA_E_fasciculatus_S2_contig9018.17113.1 vs.
Match: Putative (respiratory burst oxidase homolog n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G8C4_ECTSI)

HSP 1 Score: 112 bits (280), Expect = 2.290e-22
Identity = 78/200 (39.00%), Postives = 102/200 (51.00%), Query Frame = 1 
Query:  157 QLQRARGYHPVSLNFGRPDIEAFITGTVRGTAVVA--QWA----PPVESLPXXXXXXXXXQQTTASGLR---------------------------QMLTRLNTGQRISKRDLMEADLTVVDSSLTKSKKPSS---VSGVTGFDMPND-SATASKGSGGCVNEKSWLVLYCGANGKVEQAVQTASEGLNVTWRKEYFSKW 645
            +L+R     P+SLNFGRPDIE F+T T+RG++     +WA    PP + +           Q  AS LR                           ++L RLNT QRISKRDLM ADL+VV    +  + P S    +  +    P+         SG   + ++WLVLYCGAN KVE+AV+TA + L VTWRKEYFS W
Sbjct:  452 RLRRTPFGKPISLNFGRPDIEKFLTETIRGSSSSQHEKWAATGGPPGKIVGAAAVGDSWQPQQNASKLRASPPPSTGRHAEKERDYSDTSSRDVRKKVLARLNTNQRISKRDLMSADLSVVPGKASPPRVPRSGRVTAAASDMGSPDGVGGXXXXASGAAWDARTWLVLYCGANAKVEEAVETACDDLCVTWRKEYFSAW 651
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BLAST of mRNA_E_fasciculatus_S2_contig9018.17113.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JAC5_9PHAE)

HSP 1 Score: 101 bits (251), Expect = 8.730e-20
Identity = 80/212 (37.74%), Postives = 103/212 (48.58%), Query Frame = 1 
Query:  157 QLQRARGYHPVSLNFGRPDIEAFITGTVRGT------------------------AVVAQWAP------PVESLPXXXXXXXXXQQT---TASGLR-----QMLTRLNTGQRISKRDLMEADLTVVDSSLTKSKKPSSVSGVTGFDMPNDSATASKGSGGCV-----------NEKSWLVLYCGANGKVEQAVQTASEGLNVTWRKEYFSKW 645
            +L+R    +P+SLNFGRPDIE F+T  + G+                        AV   W P      P  S P         ++    T+S +R     ++L RLNT QRISKRDLM ADL+VV    +    P S   VT     +D  +    SGG             + ++WLVLYCGAN KVE+AV+TA + L VTWRKEYFS W
Sbjct:  123 RLRRTPFGNPISLNFGRPDIEKFLTEIIHGSSTSSSQLEEWAATGGPPGKIVGSAAVGDSWQPQQSSSKPRASPPPSTGRHAESEREYSETSSRVRTDVRKKVLARLNTNQRISKRDLMSADLSVVPGKASPPGVPRS-GRVTA--AASDMGSPDGVSGGXXXXXXXXXXAAWDPRTWLVLYCGANAKVEKAVETACDDLGVTWRKEYFSAW 331
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig9018.17113.1 vs.
Match: Putative (respiratory burst oxidase homolog protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G8C9_ECTSI)

HSP 1 Score: 85.1 bits (209), Expect = 3.210e-15
Identity = 49/97 (50.52%), Postives = 59/97 (60.82%), Query Frame = 1 
Query:  355 TRLNTGQRISKRDLMEADLTVVDSSLTKSKKPSSVSGVTGFDMPNDSATASKGSGGCVNEKSWLVLYCGANGKVEQAVQTASEGLNVTWRKEYFSKW 645
            +RL+  QRI K DLMEADL+VVDS+L K          T   +         G+    N+ +WLVLY GAN KVEQAV  AS  LNV+WRKEYF+KW
Sbjct:  122 SRLDNDQRICKMDLMEADLSVVDSNLRKGSSTPETHNPTDIYL---------GASRSFNKSTWLVLYSGANAKVEQAVIAASNRLNVSWRKEYFNKW 209
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig9018.17113.1 vs.
Match: Putative (respiratory burst oxidase homolog protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LF83_ECTSI)

HSP 1 Score: 67.0 bits (162), Expect = 6.620e-8
Identity = 50/173 (28.90%), Postives = 73/173 (42.20%), Query Frame = 1 
Query:  181 HPVSLNFGRPDIEAFI------------------TGTVRGTAVVAQWAPPVESLPXXXXXXXXXQQTTASGLRQMLTRLNTGQRISKRDLMEADLTVVDSSLTKSKKPSSVSGVTGFDMPNDSATASKGSGGCVNEKSWLVLYCGANGKVEQAVQTASEGLNVTWRKEYFSKW 645
            H V+LNFGRPDI  FI                  +GT   T + A    P  S              T     Q+   L+  Q+ S+  L+ A  + V++   +S+   + +   G   PN    +            W+V+YCGAN +VE+ + +  + LNVTWRKEYF  W
Sbjct:  586 HDVTLNFGRPDIAKFILDALNATPSATLTAGIGLSGTGATTGIGAARTSPRSS-------GNTANAATKRMDNQVTEHLDASQKKSRTVLIRASTSTVENIKKQSRDSDNAASRKGRKAPNYKPKSFP----------WIVIYCGANARVEEVIASTCDELNVTWRKEYFGGW 741
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig9018.17113.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
Putative3.130e-7985.29respiratory burst oxidase protein n=1 Tax=Ectocarp... [more]
FAD-binding3.730e-7789.88FR-type domain-containing protein n=1 Tax=Ectocarp... [more]
Putative2.290e-2239.00respiratory burst oxidase homolog n=1 Tax=Ectocarp... [more]
Uncharacterized8.730e-2037.74protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
Putative3.210e-1550.52respiratory burst oxidase homolog protein n=1 Tax=... [more]
Putative6.620e-828.90respiratory burst oxidase homolog protein n=2 Tax=... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig9018contigE_fasciculatus_S2_contig9018:1571..5201 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score282.0
Seed ortholog evalue2.4e-73
Seed eggNOG ortholog2880.D7G8C8
Model size1732
Hectar predicted targeting categoryother localisation
Exons5
EggNOG free text desc.oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
EggNOG OGsKOG0039@1,KOG0039@2759
Ec32 ortholog descriptionputative respiratory burst oxidase protein
Ec32 orthologEc-19_004000.1
Cds size648
COG Functional cat.C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig9018.17113.1prot_E_fasciculatus_S2_contig9018.17113.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig9018 1571..4117 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842556.4078116-CDS-E_fasciculatus_S2_contig9018:1570..16421696842556.4078116-CDS-E_fasciculatus_S2_contig9018:1570..1642Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig9018 1571..1642 +
1696842556.4252157-CDS-E_fasciculatus_S2_contig9018:2072..23481696842556.4252157-CDS-E_fasciculatus_S2_contig9018:2072..2348Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig9018 2073..2348 +
1696842556.4417756-CDS-E_fasciculatus_S2_contig9018:2810..29091696842556.4417756-CDS-E_fasciculatus_S2_contig9018:2810..2909Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig9018 2811..2909 +
1696842556.4522865-CDS-E_fasciculatus_S2_contig9018:3462..35971696842556.4522865-CDS-E_fasciculatus_S2_contig9018:3462..3597Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig9018 3463..3597 +
1696842556.463124-CDS-E_fasciculatus_S2_contig9018:4051..41171696842556.463124-CDS-E_fasciculatus_S2_contig9018:4051..4117Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig9018 4052..4117 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842556.4749207-UTR-E_fasciculatus_S2_contig9018:4117..52011696842556.4749207-UTR-E_fasciculatus_S2_contig9018:4117..5201Ectocarpus fasciculatus EfasUO2UTRE_fasciculatus_S2_contig9018 4118..5201 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig9018.17113.1

>prot_E_fasciculatus_S2_contig9018.17113.1 ID=prot_E_fasciculatus_S2_contig9018.17113.1|Name=mRNA_E_fasciculatus_S2_contig9018.17113.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=216bp
EGSALGVSSSHGGSALGGRRGRTEAAAARREQEQEERTTKTKKRHRRQPS
AGQLQRARGYHPVSLNFGRPDIEAFITGTVRGTAVVAQWAPPVESLPPPP
SVSPPPQQTTASGLRQMLTRLNTGQRISKRDLMEADLTVVDSSLTKSKKP
SSVSGVTGFDMPNDSATASKGSGGCVNEKSWLVLYCGANGKVEQAVQTAS
EGLNVTWRKEYFSKW*
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mRNA from alignment at E_fasciculatus_S2_contig9018:1571..5201+

Legend: polypeptideCDSUTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig9018.17113.1 ID=mRNA_E_fasciculatus_S2_contig9018.17113.1|Name=mRNA_E_fasciculatus_S2_contig9018.17113.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=3631bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig9018:1571..5201+ (Ectocarpus fasciculatus EfasUO2)
GAAGGGTCCGCGCTAGGGGTGTCTTCGTCGCACGGAGGGAGCGCGCTGGG
AGGAAGGAGGGGGCGCACCGAGGTACTCCTGCGTTGCAGTTGTCGAAGAG
TTGTTCGGCGATCGAGGCTCCGTGGTATCTGTCGCTCCCAGGTCCATGGC
GTTGTCGTTTCCTATTGTTCCAAACAGAGTATTACCGTATGAAACAGTGA
CCTAAGAACAGCAAGTTGTAGCAGGAGCATGTGGACGTGGTTCTGTCATA
GTAGCGTGTATCGCGCCTTCGGAGAGCCCTCCCGGATAAACACAACACCT
ACAACCCTGAAAAAAGATGAAACTAGCGTTTCGAGGTGAACATTTGGCAA
AAACGGGATATTGCCCTGCGAACGGTACCACACGTCACTCCCGTCGCCGG
CTTTCGTAGCAGCAGGTATGATAATGCAAAGTGCCCACTAGCAAGCTCTT
TGCTAATAATCATTTATGTCGCGAACGCGTTCGCATGTACGCCTTCCATC
AGGCTGCCGCCGCTCGGAGAGAACAAGAACAAGAGGAACGAACAACGAAG
ACGAAAAAGCGACACCGTCGCCAGCCGTCCGCCGGCCAGCTGCAGCGCGC
TCGCGGGTACCACCCGGTGTCGCTGAACTTCGGCAGGCCCGACATCGAAG
CGTTCATCACGGGTACCGTCAGGGGCACCGCCGTGGTGGCCCAGTGGGCG
CCCCCGGTCGAGTCACTGCCGCCGCCCCCTTCGGTGTCGCCGCCGCCGCA
ACAGACCACCGCCAGCGGGTTGCGACAGGTGTGTGCGTCTCGGGAGACAT
GCGTGTTATGCTGCTCGCGGTGGTTCGTTCGGGCGCGGGGGGGGGGGAGG
GGAGGGCGGGCGAGACACAGTCGCGGATGGCTATGGTAGGGAATTTGTTG
GGGATTTCTGTTCACGTCCCCACCACATTCTTCGCGTACCTCGGTTGCTC
TCGTATGGGTGCAGTAGACGGCGGAGAGAAGCGTTGTAGTTTTTTGGCGA
CGCGATGCCTTATGCGGCCTTCTGCTGCTGCGTTCCATGCGCCCCGGGTA
TTGTCACAGATGTCCTGTGTGTCACAGCCACGAGTAGCTTGGAGGTTTCA
AACGTTATATTAGGTGAGTTCTCCGCGTCACAACCATTGCATGCTGGTCC
ATACCAAGCAGAGTCGGGGTATTGTACGTGCCAGAATGTTCTTCTCCTTG
AAATACGGCTTGCGTGCGCGTGGTCTGTCTAACTGTCCAGATGCTGACGA
GGCTGAATACCGGCCAACGCATCTCGAAGAGGGATCTGATGGAAGCCGAC
CTTACCGTGGTGGACTCCAGCCTTACCAAGTCGAAGAAGGTGAGATTACG
GGGACGAATGTTGAGAACGTACATGTAGCGGGGCTGGCCTTTCTGGTAGG
AATGCACTAGAGACACGAATAGTCTAGAGCAGTAGCGGCTCCGTTGAGAT
CATCGGTTGATGAGGTTTTTGTTTTGCTTCGGGATGATTTCCTGCAGTGG
CTGGGTGGGCGTTTCGCATGTATGGCGATCATCGACGGAGTAATCGCGTG
CCGTTCCTCAAAGGCCCCTTTTGGCACGTACATGGCCACTTTCCGGGACA
TTTCGACGGTCGAAGGTCGAAGGTCGGTTCATCTTCTGAATGCCCCTACC
CCCCCCAGGTCAACGGTCTCGCAACTAAGGCATGCAAAACTTGAAAAGTA
CGCCCCCCCAGCTAGCCGAGGGCTGCTGCTGAAGCGTTTGCGATCGTCGC
CTCTCCTCTGCCTAGCTCGCGAACGCCTCCTTCCGCCAGGAGCTTCTGCC
GTACAAGCCCTCGTGTCTCGGCCATAGCAAATCTCGTTTCCTCTCCCCCT
TCTTGTTTGTCTCGTGGTGTGCGTCGTGGCCGCTGCGTGCAGCCTTCTTC
TGTGTCCGGTGTGACCGGGTTTGATATGCCGAATGATTCGGCCACCGCCA
GCAAAGGGTCCGGCGGCTGTGTAAACGAGAAGAGTTGGCTGGTGTTGTAC
TGCGGCGCCAACGGCAAGGTCGAGCAGGTGCGTCGGCGGGGGTACTCATG
GCCGTGCATGCCGTAGTCGCTAGACCATTAACCACGCAATCGCATCCCTA
CAATGCCGGGACGGAGCACGCCGGAGTTTCACCGGACCAGGCTCTAATAT
CACATCACCAAGCGCGAAGCGCCGTGAGGTATTGGTCGCATCGCCTGGAC
GGTGCGCTGCATCCTGCTAAGGGTAATGACCACGTGGAGACCGATGAAAC
CTCCGCCGATGCGAAGCCTGTAAAATGGGAGACCCGGGCAATCGTGTTCG
TCCATTGTCGGCTTGATGATAGTTGGGGGTCACCGCGCGTGAGCGCACCG
CTAGGTACTCTTTGCTGCGTGTGTGCGTTGCAGACAGGTAAGGGTGTGGA
TATTCTTGGGAGTGGTTTTGTCTCAGACTAAAATAAAAGTGTCGCTGCTT
CTGCTGCTGCTCCAACTGTTGCCATCGTTAGGCGGTGCAAACAGCAAGCG
AAGGGCTGAACGTGACATGGCGAAAGGAGTACTTCTCCAAGTGGTGAATG
CTGTGCATCCTCCTGTCATCCTCCGCCGTGACCCTCTTATTGGGGGGGGC
AAGCGCACACACACGTGTCTGGGGTGCAGAATGAACGCACATGGCCATGG
ACACAAAAACGCACATGCTTGTAGAAGCGATGAGTTCGCGGTAGGGCAAT
GGTTGGGTTGTAGGGACACCTGGCTAGCAACTGCTGTAGGGCGAGAACAC
ACAACAGCTGTGGCAACGTTAGGGGTTAGAGCGTGTGATGCTGCAGAGAC
ACGCGTCAGGATTGGAGAAACGCAACCGTTGTGTTCCCAGATCGCGGGTT
GCGTGAGGCGCGCCCAATCCCATCCATGTGGTCTCGTCTTGCAACACAAA
ACATGCGTCTTCGGCACGAGAGGAATACACGCTCGAGGAAGATAGATGTC
GACAGAAAATATGAGTTTCGGTATTGCTGATGGGGGGGCCATTGTCAGCG
TGAGTTTTCAGTACGTTTGGATGGTAGGCCACTGTTGTTGTAATTCTTGC
GCTTGAAGAATGGAGAAAATTGATGCTGACGGCCGTGGGCGCCGATCGGC
AAGTAGGTGGGCACTCAGCGCACGTCATGTGAAATAATCATGTCTTATGA
CAACTCAGAGAAGGAGTCTTTTCGGATTCACTCTTTCAAGCCCCGTTTTT
GTTCTGGCCTCCTCGTCGATGCCGACCGTCCCCTCGTGGGAGGGTCGGTC
GGTCGCACGGGCCTAAGCTAGAAACCCGGTCCTACGTAATCGCCTTAGGA
ATTGCACCGTCACCACTTGTTGAAGTACTCCTTCCGCCAGGTAACGTTCA
ACCTGTTACTCGCGGCGACAACCGCCTAGACAAGGACATCCGAATTGCGG
GGGGGGGGGATCAAAACGACGGGCTTGGGGTGGTAAGTGTTGCGTTGAAA
ACAAAAGGTTGGGTTGAGAGTGAAATCGTTGTACGAACAGGGGAAAACTA
CCTCCGCGCCGGTAGTCTCTCGCAGGCATCTCCTTTGTGAAGGGTAATGA
ATTGTCGATAGTATCCCGAACTGCAAAACGATGGAGTTTGCAATGTACAA
AGAGCCTAATGAACACACAGCGTGCATCGAC
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig9018:1571..5201+

>mRNA_E_fasciculatus_S2_contig9018.17113.1 ID=mRNA_E_fasciculatus_S2_contig9018.17113.1|Name=mRNA_E_fasciculatus_S2_contig9018.17113.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=648bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig9018:1571..5201+ (Ectocarpus fasciculatus EfasUO2)
GAAGGGTCCGCGCTAGGGGTGTCTTCGTCGCACGGAGGGAGCGCGCTGGG
AGGAAGGAGGGGGCGCACCGAGGCTGCCGCCGCTCGGAGAGAACAAGAAC
AAGAGGAACGAACAACGAAGACGAAAAAGCGACACCGTCGCCAGCCGTCC
GCCGGCCAGCTGCAGCGCGCTCGCGGGTACCACCCGGTGTCGCTGAACTT
CGGCAGGCCCGACATCGAAGCGTTCATCACGGGTACCGTCAGGGGCACCG
CCGTGGTGGCCCAGTGGGCGCCCCCGGTCGAGTCACTGCCGCCGCCCCCT
TCGGTGTCGCCGCCGCCGCAACAGACCACCGCCAGCGGGTTGCGACAGAT
GCTGACGAGGCTGAATACCGGCCAACGCATCTCGAAGAGGGATCTGATGG
AAGCCGACCTTACCGTGGTGGACTCCAGCCTTACCAAGTCGAAGAAGCCT
TCTTCTGTGTCCGGTGTGACCGGGTTTGATATGCCGAATGATTCGGCCAC
CGCCAGCAAAGGGTCCGGCGGCTGTGTAAACGAGAAGAGTTGGCTGGTGT
TGTACTGCGGCGCCAACGGCAAGGTCGAGCAGGCGGTGCAAACAGCAAGC
GAAGGGCTGAACGTGACATGGCGAAAGGAGTACTTCTCCAAGTGGTGA
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