mRNA_E_fasciculatus_S2_contig8108.16266.1 (mRNA) Ectocarpus fasciculatus EfasUO2

BLAST of mRNA_E_fasciculatus_S2_contig8108.16266.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQE5_ECTSI)

HSP 1 Score: 783 bits (2023), Expect = 2.780e-285
Identity = 393/424 (92.69%), Postives = 407/424 (95.99%), Query Frame = 1

Query:    1 MSSVRRLARALMAVSTFLRAPWGVSLAVKATINARPRPPAASTWSGYH--GSCGSGRGTTAVRRRTVATGSGEAAVLSESVDVGV-DASKNAAEGVKVSQREVLRREAWMARAEAHRERVRSLTSGSVHHDKNDPIFNFLFEYYHFKPRMLLQWTPGFDVYLLDADPSIKGCPLPQKAWESGPGAGGSYNPFGLDDNRRVQVQWMRELLQATTSRAPVFHCFGLHEWAMLYRPHGAEEAGVDARSVHQALPLRVSQDEINAMVEGRGGALACTHFDAFRFFTPEAKPLNPVQLERVGQKSNDQPGCVHANMDLFKWALRLWPLLPSEKLADALDLAVSCRMLDMRASPYDLTAHGKVPDTVVGRGGGKVGGLPPEVWDPTPVRIETEEGRKEYQQAQAELYQRSMPVRRSLMRHMDRLLDVWGR 1263
            MSS RRLARALMAVSTFLRAPWGVSLAVKATINARPRPP ASTWSGYH   SCG GRG TAVR+R VATGSGEAAVLSE VDV V DASK+AAEGV+VSQREVL REAWMARAEAHR+RVRSLT+GSVHHDK+DPIFNFLFEYYHFKPRMLLQWTPGFD YLLDADPS+KGCPLPQKAWESGPGAGGSYNPFGLD+NRRVQVQWMR+LLQATTSRAPV HCFGLHEWAMLYRP GAEEAG+DARSVHQ LPLRVSQDEINAMVEGRGGALACTHFDAFRFFTPEAKPLNPVQLERVGQ+SNDQPGCVHANMDLFKWALRLWPLLPSEKLA+AL+LAVSCRMLDMRASPYDLTAHGKVPDTVVGRGGG+VGGLPPE WDPTPVRIETEEGRKEYQQAQAELYQRSMPVRRSLMRHMDRLLDVWGR
Sbjct:    1 MSSARRLARALMAVSTFLRAPWGVSLAVKATINARPRPPGASTWSGYHRSSSCG-GRGVTAVRQRAVATGSGEAAVLSEPVDVRVEDASKSAAEGVEVSQREVLSREAWMARAEAHRKRVRSLTNGSVHHDKSDPIFNFLFEYYHFKPRMLLQWTPGFDAYLLDADPSLKGCPLPQKAWESGPGAGGSYNPFGLDNNRRVQVQWMRDLLQATTSRAPVLHCFGLHEWAMLYRPRGAEEAGIDARSVHQTLPLRVSQDEINAMVEGRGGALACTHFDAFRFFTPEAKPLNPVQLERVGQRSNDQPGCVHANMDLFKWALRLWPLLPSEKLANALELAVSCRMLDMRASPYDLTAHGKVPDTVVGRGGGRVGGLPPEAWDPTPVRIETEEGRKEYQQAQAELYQRSMPVRRSLMRHMDRLLDVWGR 423          

BLAST of mRNA_E_fasciculatus_S2_contig8108.16266.1 vs.
Match: Hypothetical (protein (Fragment) n=1 Tax=Amorphochlora amoebiformis TaxID=1561963 RepID=A0A7S0D3Z9_9EUKA)

HSP 1 Score: 243 bits (620), Expect = 3.440e-73
Identity = 133/314 (42.36%), Postives = 190/314 (60.51%), Query Frame = 1

Query:  316 WMARAEAHRERVRSLTSGSVHH----DKNDPIFNFLFEYYHFKPRMLLQWTPGFDVYLLDA---DPSIKGCPLPQKAWESGPGAGGSYNPFGLDDNRRVQ-VQWMRELLQATTSRAPVFHCFGLHEWAMLYRPHGAEEAGVDARSVHQALPLRVSQDEINAMVEGRGGALACTHFDAFRFFTPEAKPLNPVQLERVGQKSNDQPGCVHANMDLFKWALRLWPLLPSEKLADALDLAVSCRMLDMRASPYDLTAHGKVPDTVVGRGGGKVGGLPPEVWDPTPVRIETEEGRKEYQQAQAELYQRSMPVRRSLMRH 1233
            W   AE  + R+  L  G +       ++ PI+NF+FEYY FKP+MLLQ++PG  V L  A   DP++        +W       G++    L  N+ V+ ++W R+LL+ T  R P  HC+GLHEWAMLY P  ++E        +Q++PLRVSQ+ +N++++  GG + CTHFDAFRFFT +A P N VQL R  Q++ +QPGC+HANMDLFKW ++L P +PS  L D L LA+  R+LDMRASPYD+   G                   E +D +P+ IET EGR EY++ QAEL++++ P+R  L+ +
Sbjct:   98 WRREAEKEQARMLELLGGEMRSKNGKSRHHPIYNFIFEYYMFKPKMLLQYSPGLGVRLEGAQPGDPALSSRGFSHDSW-------GAWADPSLAKNKTVESLRWTRDLLKTTVDRKPELHCYGLHEWAMLYHPAESKEKPPHK---YQSIPLRVSQETVNSVIDREGG-IRCTHFDAFRFFTKDASPKNNVQLSREQQQAYEQPGCLHANMDLFKWTIKLSPWVPSSMLQDTLRLAIQARVLDMRASPYDVEGMG-------------------EGFDLSPIAIETLEGRAEYKRLQAELWKQAGPIRERLVEY 381          

BLAST of mRNA_E_fasciculatus_S2_contig8108.16266.1 vs.
Match: Uncharacterized (protein n=1 Tax=Chrysochromulina tobinii TaxID=1460289 RepID=A0A0M0J9Y2_9EUKA)

HSP 1 Score: 236 bits (603), Expect = 3.170e-71
Identity = 142/322 (44.10%), Postives = 191/322 (59.32%), Query Frame = 1

Query:  316 WMARAEAHRERVRSLTSGSVHHDKNDPIFNFLFEYYHFKPRMLLQWTPGFDVYLLDADPSIKGCPLPQKAWESGPG------AGGSYNPFGLDDNRRVQVQWMRELLQATTSRAPVFHCFGLHEWAMLYRPHGAEEAGVDARSVHQALPLRVSQDEINAMVEGRGGALACTHFDAFRFFTPEAKPLNPVQL--ERVGQKSNDQPGCVHANMDLFKWALRLWPLLPSEKLADALDLAVSCRMLDMRASPYDLTAHGKVPDTVVGRGGGKVGGLPPEVWDPTPVRIETEEGRKEYQQAQAELYQRSMPVRRSLMRHMDRLLDVW 1257
            W   AEAHR R+ SL  GS++ D ++PI+NF+F YY F  ++LL+++PG  V L       KG  + ++   +G G           +P     + R        +++AT+ RAP  +C+GLHEWAMLY+P G+        S +Q LPLRVSQ E+NA+VE     LACTHFDAFRFFT  A PLN +     R  Q   +QPGCVHA+MDLF+++L+LWP LP+E LAD L+LA++ R+LDMRASPYDL+             GG  G       D +PVRIET EGRK YQ+ Q+ L   + P+R  L+R  D  + VW
Sbjct:   19 WRDAAEAHRARMLSLIGGSLNVDLSNPIYNFIFVYYSFDQKLLLRYSPGAGVVL-------KGVGVKEEDLWTGRGYVSYGDGSARMDPMLCKPSMRNGAVTAASIMRATSGRAPHLNCYGLHEWAMLYQPPGS----TCHLSKYQELPLRVSQAELNAVVESM--PLACTHFDAFRFFTAPATPLNTISPTPSRATQAQLEQPGCVHASMDLFRYSLKLWPWLPAELLADTLELAIASRVLDMRASPYDLSQWQ----------GGAAGSF-----DLSPVRIETAEGRKLYQREQSRLASWAQPLRARLLREYDSAIAVW 312          

BLAST of mRNA_E_fasciculatus_S2_contig8108.16266.1 vs.
Match: Uncharacterized (protein n=1 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J5Y100_DIALT)

HSP 1 Score: 228 bits (581), Expect = 1.820e-67
Identity = 144/333 (43.24%), Postives = 181/333 (54.35%), Query Frame = 1

Query:  292 REVLRREAWMARAEAHRERVRSL--TSGSVHHDKNDPIFNFLFEYYHFKPRMLLQWTPGFDVYLLDADPSIKGCPLPQKAWESGP-GAGGSYNPFGLDDNRRVQVQWMRELLQATTSRAPVFHCFGLHEWAMLYRPHGAEEAGVDARSVHQA-LPLRVSQDEINAMVEGRGGALACTHFDAFRFFTPEAKPLNPV-QLERVGQKSNDQPGCVHANMDLFKWALRLWPLLPSEKLADALDLAVSCRMLDMRASPYDL----------------TAHGKVPDTVVGRGGGKVGGLPPEVWDPTPVRIETEEGRKEYQQAQAELYQRSMPVRRSLM 1227
            R VL R  W+  AEAH  RV S    S +  +D+ DP+ NF+F+YYH KP  L  W PG DV +     +  G  +P + W     G+   Y      + +RV +     +L+A   R P   C GLHEWAMLY P  A  AGV     HQ  LPLRVSQ E+NA VE     L CTHFDAFRFF PEA+PLN    L R  Q + +QPGCVHA MDLFKWALR  P + +  + + L LA+  R+LDMRASPYDL                 A G +  T   RGG  +   P  V    P+RIE+ +GR EY+  Q  LY+ S PVR +++
Sbjct:   31 RTVLERAEWLPLAEAHSARVASALGVSSTFEYDRADPLRNFVFQYYHLKPSQLRVWCPGPDVAMRGVTSADFGKLVPHERWARAVHGSATLYPAEAAPEQQRVAMTSALAVLRAAAGREPHLLCHGLHEWAMLYHPASAAAAGVPPPPRHQGGLPLRVSQAELNACVESL--PLRCTHFDAFRFFAPEARPLNREGPLSRARQAALEQPGCVHATMDLFKWALRALPFIDAALVPETLALALKARVLDMRASPYDLGAWDAHAASARAFPPAAAGGAMTSTTRTRGGRTL--APLSVEHLAPIRIESRDGRDEYRAYQLALYEESRPVRAAMI 359          

BLAST of mRNA_E_fasciculatus_S2_contig8108.16266.1 vs.
Match: 3-methyladenine (DNA glycosylase n=2 Tax=Nocardia stercoris TaxID=2483361 RepID=A0A3M2L419_9NOCA)

HSP 1 Score: 223 bits (567), Expect = 1.890e-66
Identity = 137/319 (42.95%), Postives = 173/319 (54.23%), Query Frame = 1

Query:  301 LRREAWMARAEAHRERVRSLTSGSVHH---DKNDPIFNFLFEYYHFKPRMLLQWTPGFDVYLLDADPSIKGCPLPQKAWESGPGAGGSYNPFGLDDNRRVQVQWMRELLQATTSRAPVFHCFGLHEWAMLYRPHGAEEAGVDARSVHQALPLRVSQDEINAMVEGRGGALACTHFDAFRFFTPEAKPLNPVQLERVGQKSNDQPGCVHANMDLFKWALRLWPLLPSEKLADALDLAVSCRMLDMRASPYDLTAHGKVPDTVVGRGGGKVGGLPPEVWDPTPVRIETEEGRKEYQQAQAELYQRSMPVRRSLMRHMDRLL 1248
            L +E W ARA AHRERV  L    V         P+ +FLF YY  KP  L +W PGF V L  A          + +   GP    + +P  LD  RR  V+++  LL+AT SR P   CFGLHEWAM+YR              H+ +PLR+     +A+VE    +L CTHFDA+RFFTP+A P N + L R GQ   +QPGC+HANMDL+KW  +L PL+ SE L D  +LA + R LDMRASPYDL+A G                        TPVRIET  GR EY +AQAEL   + P+R  L+   +RL+
Sbjct:    4 LPQEEWRARAAAHRERVAELIGAHVERRATGAKHPVVDFLFTYYGHKPAQLRRWHPGFGVALAGAQEYAGSRGYHEVSTTGGPAW--TADPAFLD-RRRPTVEFVATLLRATASRPPQLSCFGLHEWAMVYRTDEVR---------HEQVPLRLGAAGTDAVVESM--SLRCTHFDAYRFFTPDAVPRNTLPLTRAGQPDREQPGCLHANMDLYKWGFKLVPLIDSELLLDCFELACAARELDMRASPYDLSALGY-----------------------TPVRIETASGRAEYARAQAELADHAAPLRARLLAAAERLV 285          

BLAST of mRNA_E_fasciculatus_S2_contig8108.16266.1 vs.
Match: 3-methyladenine (DNA glycosylase n=1 Tax=Balneolaceae bacterium TaxID=2053516 RepID=A0A6A8Q2L9_9BACT)

HSP 1 Score: 221 bits (563), Expect = 8.680e-66
Identity = 120/331 (36.25%), Postives = 182/331 (54.98%), Query Frame = 1

Query:  280 KVSQREVLRREAWMARAEAHRERVRSLTSGSV----HHDKNDPIFNFLFEYYHFKPRMLLQWTPGFDVYLLDADPSIKGCPLPQKAWESGPGAGGSYNPFGLDDNRRVQVQWMRELLQATTSRAPVFHCFGLHEWAMLYRPHGAEEAGVDARSVHQALPLRVSQDEINAMVEGRGGALACTHFDAFRFFTPEAKPLNPVQLERVGQKSNDQPGCVHANMDLFKWALRLWPLLPSEKLADALDLAVSCRMLDMRASPYDLTAHGKVPDTVVGRGGGKVGGLPPEVWDPTPVRIETEEGRKEYQQAQAELYQRSMPVRRSLMRHMDRLLDVWG 1260
            K+    V+  E W  + +AH ++V +L    +    HH KN P+ +FLFEYY F+P  L +W+PG ++ L    P      LP+ +           +P      R   ++WM  LL+ + ++ P F CFG+HEWAM+Y+              H  +PLR+  DE+   VE R   L CTHFDAFRFFTP+A+PLN  +L R   +  +QPGC+H+NMDL+KWA +L+P + S  + DA +LAV  R +DM+ASPYDL  +G  P                       ++IETEEGRK+Y++ Q  ++++S P+R+ ++    +L ++ G
Sbjct:    4 KIQDLPVIPEEIWTKQKDAHEQKVDNLLGNHLKARSHHKKN-PVMDFLFEYYAFRPSALRRWSPGINIPLQYRSPEQ----LPEVSELKTEDGVAYVDPSLFPKKRHSSLKWMLGLLEKSQAKRPSFGCFGMHEWAMVYKSDNIR---------HSQVPLRMDDDELTEFVESR--PLRCTHFDAFRFFTPDARPLNRFELSREKFQDTEQPGCIHSNMDLYKWAFKLYPWINSSLILDAFELAVEARHVDMQASPYDLRDYGLEP-----------------------IKIETEEGRKQYREHQEHIFEKSRPLRQRIIEEYRKLTEIIG 295          

BLAST of mRNA_E_fasciculatus_S2_contig8108.16266.1 vs.
Match: 3-methyladenine (DNA glycosylase n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A2W4JFU3_9ACTN)

HSP 1 Score: 221 bits (563), Expect = 1.010e-65
Identity = 135/324 (41.67%), Postives = 178/324 (54.94%), Query Frame = 1

Query:  298 VLRREAWMARAEAHRERVRSLTSGSVH---HDKNDPIFNFLFEYYHFKPRMLLQWTPGFDVYLLDADPSIKGCPLPQKAWESGPGAGGSYNPFGLDDNRRVQVQWMRELLQATTSRAPVFHCFGLHEWAMLYRPHGAEEAGVDARSVHQALPLRVSQDEINAMVEGRGGALACTHFDAFRFFTPEAKPLNPVQLERVGQKSNDQPGCVHANMDLFKWALRLWPLLPSEKLADALDLAVSCRMLDMRASPYDLTAHGKVPDTVVGRGGGKVGGLPPEVWDPTPVRIETEEGRKEYQQAQAELYQRSMPVRRSLMRHMDRLLDVWG 1260
            VL  + W AR +AHR RV    +  +    H +  P+ +FLF YY ++P  L +W PG  V L+DADP+  G     + + +  G G   +   +   R   V W+R LL  T +R P   CF LHEWAM+YR    E         H   PLR+  +    +VE     L C+HFDAFRFFTPEA+PLN  QL R GQ  N+QPGC+HANMDL+K+A +L PL+PSE +A+A  LA   R LDMRASPYDL+A G                        +PVRIET EGR EY  AQ +  +R+ P+R  L+  +DR+  + G
Sbjct:   13 VLDPDTWRARRDAHRARVDRWLAEHLERRRHGRKHPVEDFLFTYYSYRPGQLRRWHPGAGVLLVDADPAEFG-----RDYRAVDG-GAMLDATAILQRRGQSVAWIRTLLANTAARRPQLSCFNLHEWAMVYRQRPEELR-------HAGWPLRLGPERTARVVEEL--RLRCSHFDAFRFFTPEARPLNQRQLSREGQLENEQPGCLHANMDLYKYAYKLCPLIPSELVAEAFALAREIRALDMRASPYDLSALGY-----------------------SPVRIETPEGRAEYVAAQRDFAERAAPLRARLIEAIDRVTALAG 298          

BLAST of mRNA_E_fasciculatus_S2_contig8108.16266.1 vs.
Match: 3-methyladenine (DNA glycosylase n=1 Tax=Myxococcales bacterium TaxID=2026763 RepID=A0A2E5HLB1_9DELT)

HSP 1 Score: 219 bits (559), Expect = 4.360e-65
Identity = 131/320 (40.94%), Postives = 177/320 (55.31%), Query Frame = 1

Query:  316 WMARAEAHRERVRSLTSGSVHHDKN-----DPIFNFLFEYYHFKPRMLLQWTPGFDVYLLD--ADPSIKGCPLPQKAWESGPGAGGSYNPFGLDDNRRVQVQWMRELLQATTSRAPVFHCFGLHEWAMLYRPHGAEEAGVDARSVHQALPLRVSQDEINAMVEGRGGALACTHFDAFRFFTPEAKPLNPVQLERVGQKSNDQPGCVHANMDLFKWALRLWPLLPSEKLADALDLAVSCRMLDMRASPYDLTAHGKVPDTVVGRGGGKVGGLPPEVWDPTPVRIETEEGRKEYQQAQAELYQRSMPVRRSLMRHMDRLLDV 1254
            W AR +AH+ R+    S  VH ++      DP+ +FLFEYY F+P  LL+W+PG  V L    AD  +K     +K + S    G   +P      RR   +W+ ELL  T +R P   C G+HEWAM+YR   A+E        H  +PLRVS +E+   VE R   + C+HFDAFRFFTP A+PLN +Q       + +QPGC+H NMD+++WA +L P +PSE LADA DLA   R +DMRASPYDL   G  P                       +R+ET EGR EYQ+ Q E++ R +P+R  L+   +RL+D 
Sbjct:   22 WRARQDAHKRRLEPFLS--VHMERRSRRIKDPVADFLFEYYAFRPTRLLRWSPGVGVVLEGQRADDFLK-----RKGFSS-TSEGVWLDPAAFPPRRRQGARWIHELLVQTQAREPFLGCAGMHEWAMVYR---ADEVR------HDQIPLRVSDEEVARFVESR--PIRCSHFDAFRFFTPAARPLNHLQPAADTMPALEQPGCIHVNMDIYRWAFKLHPWVPSELLADAFDLARDARTIDMRASPYDLRDAGLEP-----------------------LRVETVEGRAEYQRHQREIHLRGLPIRARLIAACERLIDA 299          

BLAST of mRNA_E_fasciculatus_S2_contig8108.16266.1 vs.
Match: 3-methyladenine (DNA glycosylase n=1 Tax=Rhodohalobacter sp. WB101 TaxID=2911366 RepID=UPI001EDC3145)

HSP 1 Score: 219 bits (558), Expect = 5.290e-65
Identity = 124/318 (38.99%), Postives = 172/318 (54.09%), Query Frame = 1

Query:  286 SQREVLRREAWMARAEAHRERVRSLTSGSVH---HDKNDPIFNFLFEYYHFKPRMLLQWTPGFDVYLLDADPSIKGCPLPQKAWESGPGAGGSYNPFGLDDNRRVQVQWMRELLQATTSRAPVFHCFGLHEWAMLYRPHGAEEAGVDARSVHQALPLRVSQDEINAMVEGRGGALACTHFDAFRFFTPEAKPLNPVQLERVGQKSNDQPGCVHANMDLFKWALRLWPLLPSEKLADALDLAVSCRMLDMRASPYDLTAHGKVPDTVVGRGGGKVGGLPPEVWDPTPVRIETEEGRKEYQQAQAELYQRSMPVRRSLMR 1230
            ++R VL    W A A  H E++ ++    +      + DP+ +FLFEYY F+P  L +W+PG  + L  +D S     LP+ +  +G       NP      R    +W  ELL+ +  R P+F CFG+HEWAM+YR              HQ +PLR+S DEI   VE R   L CTHFDAFRFFT +A+P+N  +L R   +  +QPGCVH NMDL+KWA +L+P + SE + +A   A+  R +DM+ASPYD T  G  P                       ++IETE GRKEY + Q E+YQ S P+R  L++
Sbjct:   10 TKRSVLDEPDWTAMASRHEEKLSAVLDPYLEKRSRQQKDPVLDFLFEYYAFRPSHLTRWSPGIGITLKYSDKSK----LPEISELNGSDGFAKLNPAFFPQKRIRSAKWTLELLENSAERKPMFGCFGMHEWAMVYRAENVR---------HQQIPLRLSDDEIAEFVESR--PLVCTHFDAFRFFTKKARPMNKHELSRETFQDTEQPGCVHTNMDLYKWAFKLYPWISSEIIREAFFNALKTRKVDMQASPYDATEFGLKP-----------------------IKIETEPGRKEYVERQMEIYQNSQPIRHRLIK 289          

BLAST of mRNA_E_fasciculatus_S2_contig8108.16266.1 vs.
Match: Uncharacterized (protein n=1 Tax=Actinocorallia herbida TaxID=58109 RepID=A0A3N1D5H6_9ACTN)

HSP 1 Score: 219 bits (557), Expect = 5.680e-65
Identity = 132/316 (41.77%), Postives = 174/316 (55.06%), Query Frame = 1

Query:  316 WMARAEAHRERVRSLTSGSV---HHDKNDPIFNFLFEYYHFKPRMLLQWTPGFDVYLLDADPSIKGCPLPQKAWESGPGAGGSYNPFGLDDNRRVQVQWMRELLQATTSRAPVFHCFGLHEWAMLYRPHGAEEAGVDARSVHQALPLRVSQDEINAMVEGRGGALACTHFDAFRFFTPEAKPLNPVQLERVGQKSNDQPGCVHANMDLFKWALRLWPLLPSEKLADALDLAVSCRMLDMRASPYDLTAHGKVPDTVVGRGGGKVGGLPPEVWDPTPVRIETEEGRKEYQQAQAELYQRSMPVRRSLMRHMDRLLDV 1254
            W AR EAHR R+  +  G +      ++ P+ +FLF YY  +P  LL W PG  V L       +G     +A+   P  G + +   L   R   + W R+LL AT SR P   CFG+HEWAM+YR  G EE        H A PLR+   E+ A+VE RG  + C+HFDAFRFFTP A+PLN +Q  R GQ+  +QPGC+HANMDL+KWA +L PL+P+E + D   LA   R +DMRASPYD+ A G  P                       +R+ET EGR EY   Q +   R+ P+R +L+ H DRLL +
Sbjct:   14 WRARREAHRARLEGMIGGYLALRRAGRSHPVEDFLFTYYGHRPGRLLTWHPGAGVVL-------EGARDFGQAYRDTP-EGTTLDLEALLPKRAKSLAWTRDLLAATASRQPHLGCFGMHEWAMVYRL-GQEEVR------HAAWPLRLPPGEVAAVVEQRG--VRCSHFDAFRFFTPPARPLNVLQPTREGQRDQEQPGCLHANMDLYKWAYKLSPLIPAELVVDCFALAREIRSVDMRASPYDMAALGHPP-----------------------IRVETAEGRAEYAARQRDFAARAEPLRAALVGHCDRLLSL 289