mRNA_E_fasciculatus_S2_contig779.15956.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig779.15956.1
Unique NamemRNA_E_fasciculatus_S2_contig779.15956.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig779.15956.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KT30_9PHAE)

HSP 1 Score: 212 bits (540), Expect = 9.780e-60
Identity = 101/108 (93.52%), Postives = 105/108 (97.22%), Query Frame = 1 
Query:    1 DIANHFCECAGFDADFRRRYPSDKQRQTFLRAYLEAARPGALQNAVSANNVISELTRLVDRYTLASHLTWALWAVIQANTSEIEDFDFAGYATKRLDGYAFHKGEFYA 324
            DIANHFCECAGFDADFRRRYPSDKQRQ FLRAY+EAARPGALQ A+SA+NVISEL RLV+RYTLASHLTWALWAVIQANTSEIEDFDFAGYATKRLDGYAFHKGEFYA
Sbjct:  329 DIANHFCECAGFDADFRRRYPSDKQRQAFLRAYVEAARPGALQKALSADNVISELARLVNRYTLASHLTWALWAVIQANTSEIEDFDFAGYATKRLDGYAFHKGEFYA 436
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BLAST of mRNA_E_fasciculatus_S2_contig779.15956.1 vs.
Match: Choline/ethanolamine (kinase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G1J0_ECTSI)

HSP 1 Score: 205 bits (522), Expect = 3.560e-57
Identity = 99/108 (91.67%), Postives = 102/108 (94.44%), Query Frame = 1 
Query:    1 DIANHFCECAGFDADFRRRYPSDKQRQTFLRAYLEAARPGALQNAVSANNVISELTRLVDRYTLASHLTWALWAVIQANTSEIEDFDFAGYATKRLDGYAFHKGEFYA 324
            DIANHFCECAGFDADFRRRYPSDKQRQ FLRAY+EAARP ALQNA+SA+NVISEL RLVDRYTLASHLTWALWAVIQANTSEIE FDFAGYATKRLDGYAFHK  FYA
Sbjct:  323 DIANHFCECAGFDADFRRRYPSDKQRQAFLRAYVEAARPEALQNALSADNVISELARLVDRYTLASHLTWALWAVIQANTSEIEGFDFAGYATKRLDGYAFHKEAFYA 430
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BLAST of mRNA_E_fasciculatus_S2_contig779.15956.1 vs.
Match: Hypothetical (protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XSL1_HETAK)

HSP 1 Score: 87.8 bits (216), Expect = 1.070e-15
Identity = 45/110 (40.91%), Postives = 63/110 (57.27%), Query Frame = 1 
Query:    1 DIANHFCECAGFDADFRRRYPSDKQRQTFLRAYLEAARPGALQNAVSANNV---ISELTRLVDRYTLASHLTWALWAVIQANTSEIEDFDFAGYATKRLDGYAFHKGEFY 321
            DIANHFCE AGFD D +  +P+ +Q+  FL  Y+  A P       S   +   + EL + +  + LASHL W  WA++QA  S I DFD+ GYA  R +G+ +H   F+
Sbjct:   74 DIANHFCEYAGFDFDIKNHFPNKEQQLYFLEKYVSHATPELHAKLTSEGRLGILLEELYQELKYFVLASHLWWGYWAIMQAKHSPI-DFDYMGYARLRFEGFDYHTCLFF 182
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BLAST of mRNA_E_fasciculatus_S2_contig779.15956.1 vs.
Match: Uncharacterized (protein n=1 Tax=Chytriomyces confervae TaxID=246404 RepID=A0A507FFN8_9FUNG)

HSP 1 Score: 87.4 bits (215), Expect = 4.330e-15
Identity = 48/108 (44.44%), Postives = 68/108 (62.96%), Query Frame = 1 
Query:    1 DIANHFCECAGFDADFRRRYPSDKQRQTFLRAYLEAARPGALQNAVSANNVISELTRLVDRYTLASHLTWALWAVIQANTSEIEDFDFAGYATKRLDGYAFHKGEFYA 324
            DI NHFCE AGFD ++   YP +  ++ +LR YLE A  G+  + V+   V S L   V++Y LA+HL W +WA++QA  S++ DFD+AGY+  R+D Y   K  F A
Sbjct:  253 DIGNHFCEYAGFDCEWS-LYPDESFQKVWLRRYLETAN-GSESSPVTEEQV-SMLYAEVNKYALAAHLYWGIWALVQAEISDL-DFDYAGYSKLRIDEYFRRKSVFLA 356
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BLAST of mRNA_E_fasciculatus_S2_contig779.15956.1 vs.
Match: Choline/ethanolamine (kinase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LQ37_ECTSI)

HSP 1 Score: 87.8 bits (216), Expect = 5.400e-15
Identity = 51/129 (39.53%), Postives = 64/129 (49.61%), Query Frame = 1 
Query:    1 DIANHFCECAGFDADFRRRYPSDKQRQTFLRAYLEAAR--------PGALQNAVSANNV--------------ISELTRLVDRYTLASHLTWALWAVIQANTSEIEDFDFAGYATKRLDGYAFHKGEFY 321
            DIANHFCE AGFD++F + YP+   +  FL AY+ A          PG         +               +  L   V+R+ L SHL W+LWAV+QA  S IE FDF  YA  RL GY  HK  F+
Sbjct:  269 DIANHFCEHAGFDSNFEKSYPTADTQAAFLTAYVRAVTTPEPSTDMPGEADETTEEESTDASTDSPRSDEESYVEALRTEVNRWALPSHLWWSLWAVVQARYSPIE-FDFMDYARLRLAGYRLHKKSFF 396
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BLAST of mRNA_E_fasciculatus_S2_contig779.15956.1 vs.
Match: Uncharacterized (protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482UQX4_9ARCH)

HSP 1 Score: 87.8 bits (216), Expect = 8.160e-15
Identity = 51/110 (46.36%), Postives = 62/110 (56.36%), Query Frame = 1 
Query:    1 DIANHFCECAGFDADFRRRYPS-DKQRQTFLRAYLEAARPGALQNA--VSANNVISELTRLVDRYTLASHLTWALWAVIQANTSEIEDFDFAGYATKRLDGYAFHKGEFY 321
            D+ANHFCE AGFD D  R YP+ D QR  F     + AR GA   A  +       EL  + +R+ LASH  W LWA++QA  S I DFDF GY   R  GY  HK +F+
Sbjct:  344 DVANHFCEHAGFDFDLERWYPAPDLQRHWFQVYTTKLARGGAGVPAPLMEQEAFWDELYLVANRFALASHYWWGLWAIVQAQHSAI-DFDFLGYCIMRFKGYEKHKHQFF 452
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BLAST of mRNA_E_fasciculatus_S2_contig779.15956.1 vs.
Match: Choline/ethanolamine (kinase n=1 Tax=Rhizoclosmatium globosum TaxID=329046 RepID=A0A1Y2C6Y5_9FUNG)

HSP 1 Score: 85.5 bits (210), Expect = 2.180e-14
Identity = 46/108 (42.59%), Postives = 68/108 (62.96%), Query Frame = 1 
Query:    1 DIANHFCECAGFDADFRRRYPSDKQRQTFLRAYLEAARPGALQNAVSANNVISELTRLVDRYTLASHLTWALWAVIQANTSEIEDFDFAGYATKRLDGYAFHKGEFYA 324
            DI NHFCE AGFD ++   YP+ + ++ +LR YL +A P    +     + I++L   V++Y LA+HL W +WA++QA  S++ DFD+AGYA  R+D Y   K  F A
Sbjct:  269 DIGNHFCEHAGFDCEWD-LYPNQEFQRKWLREYLGSAHP----DQEVTEDEITKLYAEVNKYALAAHLYWGIWALVQAEISDL-DFDYAGYAKLRIDEYFRRKTGFLA 370
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BLAST of mRNA_E_fasciculatus_S2_contig779.15956.1 vs.
Match: Choline/ethanolamine (kinase n=1 Tax=Basidiobolus meristosporus CBS 931.73 TaxID=1314790 RepID=A0A1Y1XTJ2_9FUNG)

HSP 1 Score: 85.1 bits (209), Expect = 2.380e-14
Identity = 46/108 (42.59%), Postives = 65/108 (60.19%), Query Frame = 1 
Query:    1 DIANHFCECAGFDADFRRRYPSDKQRQTFLRAYLEAARPGALQNAVSANNVISELTRLVDRYTLASHLTWALWAVIQANTSEIEDFDFAGYATKRLDGYAFHKGEFYA 324
            DI NHF E AGF+ D+  RYP+ + ++ +LR YLE+A     Q    +   +  L R V+++ LASH+ W LWA++QA  S+I DFD+  YA  R + Y   K EF A
Sbjct:  249 DIGNHFAEYAGFECDYS-RYPTREYQKVWLRKYLESA-----QEHSPSEEEVESLYREVNQFALASHIYWGLWALVQAQLSDI-DFDYMSYAVLRFNEYYRKKEEFLA 349
[Close]
BLAST of mRNA_E_fasciculatus_S2_contig779.15956.1 vs.
Match: Uncharacterized (protein n=1 Tax=Hyaloperonospora arabidopsidis (strain Emoy2) TaxID=559515 RepID=M4BDE0_HYAAE)

HSP 1 Score: 83.2 bits (204), Expect = 2.970e-14
Identity = 47/109 (43.12%), Postives = 60/109 (55.05%), Query Frame = 1 
Query:    1 DIANHFCECAGFDADFRRRYPSDKQRQTFLRAYLEAARP---GALQNAVSANNVISELTRLVDRYTLASHLTWALWAVIQANTSEIEDFDFAGYATKRLDGYAFHKGEF 318
            D ANHFCE  GF+ D    YPS +++  F RAY+  A P     L+    +      L   V RYTLASHL W  WA++QA  S+I DFDF  YA KR + +  H+  F
Sbjct:  145 DFANHFCENCGFELDLA-FYPSMEKQFAFFRAYMSTAAPDLLAELETNRESKAFFRALYNAVTRYTLASHLFWGYWALVQAAHSKI-DFDFLAYAAKRFNTFDVHRELF 251
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BLAST of mRNA_E_fasciculatus_S2_contig779.15956.1 vs.
Match: Predicted (protein n=1 Tax=Phaeodactylum tricornutum (strain CCAP 1055/1) TaxID=556484 RepID=B7G1A1_PHATC)

HSP 1 Score: 83.2 bits (204), Expect = 2.010e-13
Identity = 45/110 (40.91%), Postives = 64/110 (58.18%), Query Frame = 1 
Query:    1 DIANHFCECAGFD---ADFRRRYPSDKQRQTFLRAYLEAARPGALQNAVSANNVISELTRLVDRYTLASHLTWALWAVIQANTSEIEDFDFAGYATKRLDGYAFHKGEFY 321
            DIAN FCE    +   AD+ R YPS+  +  F RAYL+     +L  A      ++ +   V +YTL SHL WA+W+++Q N S+IE FD+  YA  R+DGY   K +F+
Sbjct:  330 DIANTFCEFCDMNNMRADYEREYPSEDVQNEFFRAYLKDLECSSLL-AGQQEEFLTAMRLHVGKYTLLSHLGWAVWSLVQHNLSDIE-FDYLAYAQHRMDGYELFKAKFW 437
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig779.15956.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
Uncharacterized9.780e-6093.52protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
Choline/ethanolamine3.560e-5791.67kinase n=1 Tax=Ectocarpus siliculosus TaxID=2880 R... [more]
Hypothetical1.070e-1540.91protein n=1 Tax=Heterosigma akashiwo TaxID=2829 Re... [more]
Uncharacterized4.330e-1544.44protein n=1 Tax=Chytriomyces confervae TaxID=24640... [more]
Choline/ethanolamine5.400e-1539.53kinase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LQ37_... [more]
Uncharacterized8.160e-1546.36protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A48... [more]
Choline/ethanolamine2.180e-1442.59kinase n=1 Tax=Rhizoclosmatium globosum TaxID=3290... [more]
Choline/ethanolamine2.380e-1442.59kinase n=1 Tax=Basidiobolus meristosporus CBS 931.... [more]
Uncharacterized2.970e-1443.12protein n=1 Tax=Hyaloperonospora arabidopsidis (st... [more]
Predicted2.010e-1340.91protein n=1 Tax=Phaeodactylum tricornutum (strain ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig779contigE_fasciculatus_S2_contig779:3630..6038 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score205.3
Seed ortholog evalue1.5e-50
Seed eggNOG ortholog2880.D7G1J0
Model size1524
KEGG rclassRC00002,RC00017
KEGG koko:K00894
KEGG ReactionR01468
KEGG Pathwayko00564,ko01100,map00564,map01100
KEGG ModuleM00092
Hectar predicted targeting categoryother localisation
GOsGO:0005575,GO:0005623,GO:0005886,GO:0006629,GO:0006644,GO:0006793,GO:0006796,GO:0008150,GO:0008152,GO:0008610,GO:0008654,GO:0009058,GO:0009987,GO:0016020,GO:0019637,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044464,GO:0071704,GO:0071944,GO:0090407,GO:1901576
Exons3
EggNOG free text desc.ethanolamine kinase activity
EggNOG OGsCOG0510@1,KOG4720@2759
EC2.7.1.82
Cds size327
COG Functional cat.M
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842440.5747588-CDS-E_fasciculatus_S2_contig779:3629..37671696842440.5747588-CDS-E_fasciculatus_S2_contig779:3629..3767Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig779 3630..3767 +
1696842440.5967312-CDS-E_fasciculatus_S2_contig779:4076..41901696842440.5967312-CDS-E_fasciculatus_S2_contig779:4076..4190Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig779 4077..4190 +
1696842440.607146-CDS-E_fasciculatus_S2_contig779:4768..48431696842440.607146-CDS-E_fasciculatus_S2_contig779:4768..4843Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig779 4769..4843 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842440.6173632-UTR-E_fasciculatus_S2_contig779:4843..60381696842440.6173632-UTR-E_fasciculatus_S2_contig779:4843..6038Ectocarpus fasciculatus EfasUO2UTRE_fasciculatus_S2_contig779 4844..6038 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig779.15956.1prot_E_fasciculatus_S2_contig779.15956.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig779 3630..4843 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig779.15956.1

>prot_E_fasciculatus_S2_contig779.15956.1 ID=prot_E_fasciculatus_S2_contig779.15956.1|Name=mRNA_E_fasciculatus_S2_contig779.15956.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=109bp
DIANHFCECAGFDADFRRRYPSDKQRQTFLRAYLEAARPGALQNAVSANN
VISELTRLVDRYTLASHLTWALWAVIQANTSEIEDFDFAGYATKRLDGYA
FHKGEFYA*
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mRNA from alignment at E_fasciculatus_S2_contig779:3630..6038+

Legend: CDSpolypeptideUTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig779.15956.1 ID=mRNA_E_fasciculatus_S2_contig779.15956.1|Name=mRNA_E_fasciculatus_S2_contig779.15956.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=2409bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig779:3630..6038+ (Ectocarpus fasciculatus EfasUO2)
GACATCGCCAACCACTTCTGCGAGTGCGCCGGCTTCGATGCTGATTTCCG
GAGGCGTTATCCTTCCGACAAGCAGCGGCAAACATTCCTTAGGGCGTACT
TGGAGGCGGCAAGACCTGGGGCGCTTCAAAACGCGGTGGTGGGTCAGGGA
CTGCAAACGCGTGGTCTGGAGGTGGCAGTGGTTTGTTGGGGGTGAATTTT
GGTTTGGTTGATGGTGCTGTTTTGTTTTTTTTCCTGCTGCTGCTGTTGGT
GGTGATAGTTTCGATGCTGCTATTGTGTGTTCCCTGGTATCGATCCCGAT
CGCCTTTGTAGGTGCCGTTGCTGCCTCTATGGGAGTGTTTTCTACTTCTA
GTCTGTCGCGGTTTTCTAACGCGTGTGTCCTAGTCAGCGTTGGCGTCAAC
CCCCTCCCTCCCGCCTCCCTCGATGTATGACCTCCCGCGGCCCGCAGTCT
GCCAACAACGTCATTAGTGAGCTCACGAGGCTCGTCGACCGGTACACACT
AGCCAGCCACCTTACGTGGGCCCTGTGGGCAGTGATCCAGGCAAACACTT
CGGAGATAGAGGTGGGTCCTTGTCATTTGTTGTTGTTGTTGTCGTTGCCG
TGCCTGCCTTCACGTGGAAGCGTTGGCGGCCGTGTGAATACAATAGCGCC
ACGCAGCTGCTTGTCGCCGTGTTCGTTGTTTTTGCTATTGTTGTGGTCGT
CGTAGTCGCAGAAGTTTTCGTTCTTCCTTGGTGTTGCCAAAGCGTCTGTT
GCTGTGATTTGTTTAAAACGTCTGTCGCTGTGATTTGTTTGAAACCCAGT
TGTTAGAGGTGGTTGCGCTTGTTATTCGAATGTTTTTTTGTTGTTATCTC
GGAATTTGACCGCGGGCTCCGTCACTTGCTGCTTCGTTCGAACCGCCGAA
CATCCCAGCATCCCTCAATCGCTTGGTCCTAACAAATCTGCCCTGGTCCG
CGAGTTCCACCAAGGTGGAAGTGCCTCGTCCTTGCTATTCCGTCGAGGGC
CTGTCGTTCCTAAGAACAATGTGACCATGCGTACAGATGAGTGTTCGTTT
CTCCCCGCTTGCCAGATGTGTTTGGTTTCGAGGTGGAAGATCTCGGCTCT
CTCCCTCTCTCCTCATTTTTCGTGACAATCGCTTTGCAGGACTTCGATTT
CGCGGGCTACGCCACGAAGCGGCTGGACGGTTACGCCTTCCACAAGGGGG
AGTTCTACGCGTAATGTCCCTTTCTTTCTTCGTCTTGGATCTTGGAGAGG
GCGATGCCCTGAGAATCGTGGGAAGTTCAGCGCAATTTTTTCTGTCGCTT
GTCTTAATGTTTACGGCAGCTACTGGGCTTCCAGAGACTTGCCATCGGGC
CATAAACGCTCAGTGTTTTGGTGTTTCATGGGTGCTGGCTTCGTGAATTT
GTGTAGAACACTAGAGACCGCCAACGGCAACGAGACATCGGAGGTTGTAA
TTTCGGCAACGCTGGCACCCGGAGTAGCAGTCGGCCACGCCCGGGCGGTT
GTGGTTGAAGCTGACAGTGGCCTTGGTTACAAGGGTCACCCGAGGTTTGA
ACCCGCAAGTCCTTGGCAACTCCGGTGGCGCGATGGTTGACCTGTGTGAG
ATTCTGATGTACTAGAACGGCGCCAACAGCTGGAGTTAGTGGGTGGGTGG
TGTACATGTAGATGGGATGTAGATGTAGACGTTTTTTTCTTGTTCAATCA
ACCGAGGACGTGCCAACAATTTATAGACATTAGAAGAGAGGCGACGTGGA
GGCTAGGGCAACTAGGGGAAACGAGAAAGAAAGACGGGAGTGGGGGCAGT
GAGTCAGATGTGCGGCGCCACGATTCGACTTCCGCTGTCAAGCAGCTTGC
GACGTCTGTCTCCTGCGCCCGCTACAGTCGCGCGGAGGGAAATGGGTATT
TGTCTGGGTTTACCTTGTTACGTTGAAAGACGGTAGACGTTTCGCTTACG
GCATATCCCCAGCAATAGAGCGCCGGGGGCTACGGTACACCACACGGTTC
TCCTGTGTGACAATAGTCAGGAGCGTATGCTGATAGACTCAGGGTGTCAA
TGGGACTAGAGCTGCTTGTTTTGTATTTTTCTCGTCTGGTGTTTTGTGCT
AACAAATTGTAGCAGCGTGGCACCATATTGGGCGCAGTGGCGGCAGAGGG
GGGCTTTGGGCGGTAATAGCGCCAACGCGACATTGTCTGCGGCCCTTGGT
TTACGAGGTACGACGCACGTAGCTTTTTCGCAATGTGTGTGTGCGGGTGT
GGGAGAGGCGTTCATGATATTTTCTTGCTTGCTTTGTTGGAACATTTGTT
TCTGTCTTGCTGGGGATGCTCCCGCTAGACTGTTGTTGTACCACAGGGAA
AGGAAAAGTCGGTGCGAACGTAAAAGTTGGCATCGGTATGCGCTGTTGCC
TGGTTTTCG
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig779:3630..6038+

>mRNA_E_fasciculatus_S2_contig779.15956.1 ID=mRNA_E_fasciculatus_S2_contig779.15956.1|Name=mRNA_E_fasciculatus_S2_contig779.15956.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=327bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig779:3630..6038+ (Ectocarpus fasciculatus EfasUO2)
GACATCGCCAACCACTTCTGCGAGTGCGCCGGCTTCGATGCTGATTTCCG
GAGGCGTTATCCTTCCGACAAGCAGCGGCAAACATTCCTTAGGGCGTACT
TGGAGGCGGCAAGACCTGGGGCGCTTCAAAACGCGGTGTCTGCCAACAAC
GTCATTAGTGAGCTCACGAGGCTCGTCGACCGGTACACACTAGCCAGCCA
CCTTACGTGGGCCCTGTGGGCAGTGATCCAGGCAAACACTTCGGAGATAG
AGGACTTCGATTTCGCGGGCTACGCCACGAAGCGGCTGGACGGTTACGCC
TTCCACAAGGGGGAGTTCTACGCGTAA
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