mRNA_E_fasciculatus_S2_contig7657.15825.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E_fasciculatus_S2_contig7657.15825.1
Unique NamemRNA_E_fasciculatus_S2_contig7657.15825.1
TypemRNA
OrganismEctocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)
Homology
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BLAST of mRNA_E_fasciculatus_S2_contig7657.15825.1 vs.
Match: Sulfotransfer_1 (domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G7A7_ECTSI)

HSP 1 Score: 205 bits (521), Expect = 3.410e-60
Identity = 100/114 (87.72%), Postives = 106/114 (92.98%), Query Frame = 1 
Query:  688 MAHHPALRWVSNLRMIGRVAEEAHVLDEMGSEGFQQMLGEGPSGKYGQTAPVAEESDKVIDY---TPHCSIKAEVPYRILDIYGEAARDGSNKYLVQLRDPVARAISSWTSKFD 1020
            MAHHPAL+WVSNL MIGRVAEEAHVLDE+G E FQQMLG+GP+GKYGQTAP AEESDKVID    TPHCSIKAEVPYRILDIYG+AARDGSNKYL+QLRDPVARAISSWTSKFD
Sbjct:    1 MAHHPALQWVSNLHMIGRVAEEAHVLDELGKEDFQQMLGQGPTGKYGQTAPFAEESDKVIDCAADTPHCSIKAEVPYRILDIYGDAARDGSNKYLIQLRDPVARAISSWTSKFD 114
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BLAST of mRNA_E_fasciculatus_S2_contig7657.15825.1 vs.
Match: Sulfotransferase, (C-terminal Sulfotransferase, N-terminal n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G7B1_ECTSI)

HSP 1 Score: 145 bits (367), Expect = 4.070e-37
Identity = 78/147 (53.06%), Postives = 95/147 (64.63%), Query Frame = 1 
Query:  589 RCWADHETGRHRCQANVFLFGVSKCGTTSLASWMAHHPALRWVSNL-RMIGRVAEEAHVLDEMGSEGFQQMLGEGPSGKYGQTAPVAEESDKVIDYTPHCSIKAEVPYRILDIYGEAARDGSNKYLVQLRDPVARAISSWTSKFDRE 1026
            RC+        RC ANV+ FGVSKCGTTSLA WMA HPAL WVSN+     +   EAHV+ EM +    +         Y  TAP+A ++D VIDYTPH SI AEVPYRI D+YG+ + DG+ KY+  LR+PVAR ISSW  K+D E
Sbjct:   70 RCFVLEGEDSSRCHANVYFFGVSKCGTTSLAHWMARHPALSWVSNVWHTRHKQGGEAHVM-EMSTIKDNEST-------YALTAPMAAQTDTVIDYTPHYSILAEVPYRIRDMYGKTSYDGTVKYIAVLREPVARTISSWQFKYDLE 208
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BLAST of mRNA_E_fasciculatus_S2_contig7657.15825.1 vs.
Match: Sulfotransfer_1 (domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JZE4_9PHAE)

HSP 1 Score: 146 bits (368), Expect = 2.150e-36
Identity = 75/145 (51.72%), Postives = 93/145 (64.14%), Query Frame = 1 
Query:  589 RCWADHETGRHRCQANVFLFGVSKCGTTSLASWMAHHPALRWVSNL-RMIGRVAEEAHVLDEMGSEGFQQMLGEGPSGKYGQTAPVAEESDKVIDYTPHCSIKAEVPYRILDIYGEAARDGSNKYLVQLRDPVARAISSWTSKFD 1020
            RC+        RC ANV+ FGVSKCGTTSLA WMA HPAL+WVSN      +   EAHV++    +  +          Y  TAP+  ++DKVIDYTPH SI AEVPYRI D+YG+ + DG+ KY+  LR+PVAR ISSW  K+D
Sbjct:  110 RCFVLEGEDSSRCHANVYFFGVSKCGTTSLAHWMARHPALQWVSNTCHTQHKQGGEAHVMEMSTIKNSEST--------YALTAPMTAQTDKVIDYTPHYSILAEVPYRIKDMYGKTSYDGTVKYIAVLREPVARTISSWEFKYD 246
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BLAST of mRNA_E_fasciculatus_S2_contig7657.15825.1 vs.
Match: Sulfotransferase (n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LLL8_ECTSI)

HSP 1 Score: 122 bits (306), Expect = 1.700e-27
Identity = 70/148 (47.30%), Postives = 85/148 (57.43%), Query Frame = 1 
Query:  589 RCWADHETGRHRCQANVFLFGVSKCGTTSLASWMAHHPALRWVSNLRMIGRVAE----EAHVLDE--MGSEGFQQMLGEGPSGKYGQTAPVAEESDKVIDYTPHCSIKAEVPYRILDIYGEAARDGSNKYLVQLRDPVARAISSWTSK 1014
            RC+   E G HRC   VF FG SKCGTTSLA W+ HHPA  WV+N R   ++ E    EAHV D+   G   F++ +      K   T P +   D VIDYTPH    AE P+RI DIYG   R+   K++V LR+P  RAISSW  K
Sbjct:  158 RCFVGAE-GDHRCYPTVFFFGTSKCGTTSLARWLDHHPATHWVANPRRPHQIEETGVKEAHVFDDPPKGDPAFEEGM---HHEKLFITTPKSGAEDVVIDYTPHYIAVAETPHRIADIYG--GRESGLKFIVTLREPAGRAISSWEFK 299
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BLAST of mRNA_E_fasciculatus_S2_contig7657.15825.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L1A9_9PHAE)

HSP 1 Score: 109 bits (273), Expect = 3.040e-23
Identity = 68/149 (45.64%), Postives = 83/149 (55.70%), Query Frame = 1 
Query:  589 RCWADHETGRHRCQANVFLFGVSKCGTTSLASWMAHHPALRWVSNLRMIGRVAEEAHVLDEMGSEGFQQMLGEGPSGKYGQTAPVAEESDKVIDYTPHCSIKAEVPYRILDIYGEAA--RDGSN-KYLVQLRDPVARAISSWTSKFDRE 1026
            RCW D E G  RC   VF FGVSKCGTTSL++W++ HP   +V           EAHV D       +   G    GK    +PVA   D VID+TP+ +I  E P RI D Y +A   R  SN ++LV LR+PVAR ISSW  K +RE
Sbjct:  244 RCWTD-EAGNGRCLPTVFFFGVSKCGTTSLSNWLSSHPRAVFVPPPE--AHDYREAHVFDNTP----RYWHGVSDRGKRESLSPVASSRDTVIDFTPNYAIVGEAPLRIYDYYHQAGGGRLRSNLRFLVVLREPVARTISSWQYKSERE 385
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BLAST of mRNA_E_fasciculatus_S2_contig7657.15825.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FHD4_ECTSI)

HSP 1 Score: 103 bits (257), Expect = 1.230e-20
Identity = 65/145 (44.83%), Postives = 80/145 (55.17%), Query Frame = 1 
Query:  589 RCWADHETGRHRCQANVFLFGVSKCGTTSLASWMAHHPALRWVSNLRMIGRVAEEAHVLDEMGSEGFQQMLGEGPSGKYGQTAPVAEESDKVIDYTPHCSIKAEVPYRILDIYGEAA--RDGSN-KYLVQLRDPVARAISSWTSK 1014
            RCW D E G  RC   VF FGVSKCGTTSL++W++ HP   +V           EAHV D       +   G    GK    +P+A   D VID+TP+ +I  E P RI D Y +A   R  SN ++LV LR+PVAR ISSW  K
Sbjct:  246 RCWTD-EAGNGRCLPTVFFFGVSKCGTTSLSNWLSSHPRAVFVPPPE--AHDYREAHVFDNTP----KYWHGVSDRGKRESLSPMASWRDTVIDFTPNYAIVGEAPLRIYDYYHQAGGGRLRSNLRFLVVLREPVARTISSWEYK 383
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BLAST of mRNA_E_fasciculatus_S2_contig7657.15825.1 vs.
Match: P-loop (containing nucleoside triphosphate hydrolase protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YNP6_9STRA)

HSP 1 Score: 85.1 bits (209), Expect = 4.090e-15
Identity = 53/135 (39.26%), Postives = 74/135 (54.81%), Query Frame = 1 
Query:  613 GRHRCQANVFLFGVSKCGTTSLASWMAHHPALRWVSNLRMIGRVAEEAHVLDEMGSEGFQQMLGEGPSGKYGQTAPVAEE-SDKVIDYTPHCSIKAEVPYRILDIYGEAARDGSNKYLVQLRDPVARAISSWTSK 1014
            G+ RC  NV  FGVSK GTTSLA W++ HP ++++      GR +E    L        ++        +  + AP A+  SD V+DYTPH ++  EVPYRI  +   +    S K++V LR+P  RAISSW  K
Sbjct:    2 GQTRCLPNVVFFGVSKAGTTSLAHWLSQHPQVKFMG-----GRPSESRAYLKGRTLADIRE--------RSKRLAPSADPVSDVVVDYTPHYAMVPEVPYRICHLLDCS----STKFIVLLREPAERAISSWRFK 119
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BLAST of mRNA_E_fasciculatus_S2_contig7657.15825.1 vs.
Match: Sulfotransfer_1 (domain-containing protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482UY40_9ARCH)

HSP 1 Score: 54.7 bits (130), Expect = 5.830e-5
Identity = 47/152 (30.92%), Postives = 68/152 (44.74%), Query Frame = 1 
Query:  589 RCWADHETGRHRCQANVFLFGVSKCGTTSLASWMAHHPALRWVSNLRMIGRVAEEAHVLDEMGSEGFQQMLGEGPSGKYGQTAPVAEESDKVIDYTPHCSIKAEVPYRILD-----IYGEAARDGSNKYLVQLRDPVARAISSWTSKFDREF 1029
            RC   + T   RC  NVFL G SK GTTSL  +++  P + +        +   E H  D   S  +     E  + ++     V + +  VI YTP       VPY I D     +   + R+   K+++ LRDPV RA+SS+  K    F
Sbjct:   53 RCKVFNST-HFRCLPNVFLIGSSKAGTTSLVLYLSKLPNVYFSFREETSQKDNSEQHRFDRA-SYPYSNKEVE-LAHEWASRPLVTDPNAVVIHYTPQYLYSPNVPYEIRDFIKLVLKKASVRNYDVKFVMLLRDPVDRAMSSYWFKNSHMF 201
The following BLAST results are available for this feature:
BLAST of mRNA_E_fasciculatus_S2_contig7657.15825.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 8
Match NameE-valueIdentityDescription
Sulfotransfer_13.410e-6087.72domain-containing protein n=1 Tax=Ectocarpus silic... [more]
Sulfotransferase,4.070e-3753.06C-terminal Sulfotransferase, N-terminal n=1 Tax=Ec... [more]
Sulfotransfer_12.150e-3651.72domain-containing protein n=1 Tax=Ectocarpus sp. C... [more]
Sulfotransferase1.700e-2747.30n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LLL8_ECTSI[more]
Uncharacterized3.040e-2345.64protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
Uncharacterized1.230e-2044.83protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
P-loop4.090e-1539.26containing nucleoside triphosphate hydrolase prote... [more]
Sulfotransfer_15.830e-530.92domain-containing protein n=1 Tax=archaeon TaxID=1... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E_fasciculatus_S2_contig7657contigE_fasciculatus_S2_contig7657:4166..6865 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO22022-07-07
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score122.5
Seed ortholog evalue3.9e-25
Seed eggNOG ortholog2880.D8LLL8
Model size1041
Hectar predicted targeting categorysignal peptide
Exons3
EggNOG free text desc.heparan sulfate sulfotransferase activity
EggNOG OGsKOG3704@1,KOG3704@2759
Ec32 ortholog descriptionP-loop containing nucleoside triphosphate hydrolase
Ec32 orthologEc-04_003540.1
Cds size1041
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1696842427.8706965-CDS-E_fasciculatus_S2_contig7657:4165..48291696842427.8706965-CDS-E_fasciculatus_S2_contig7657:4165..4829Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7657 4166..4829 +
1696842427.8865662-CDS-E_fasciculatus_S2_contig7657:5702..59091696842427.8865662-CDS-E_fasciculatus_S2_contig7657:5702..5909Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7657 5703..5909 +
1696842427.9026954-CDS-E_fasciculatus_S2_contig7657:6695..68651696842427.9026954-CDS-E_fasciculatus_S2_contig7657:6695..6865Ectocarpus fasciculatus EfasUO2CDSE_fasciculatus_S2_contig7657 6696..6865 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E_fasciculatus_S2_contig7657.15825.1prot_E_fasciculatus_S2_contig7657.15825.1Ectocarpus fasciculatus EfasUO2polypeptideE_fasciculatus_S2_contig7657 4166..6865 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E_fasciculatus_S2_contig7657.15825.1

>prot_E_fasciculatus_S2_contig7657.15825.1 ID=prot_E_fasciculatus_S2_contig7657.15825.1|Name=mRNA_E_fasciculatus_S2_contig7657.15825.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=347bp
MAAGATAAPMLLVSLLSFWGARRSGGGGGPLLAGAQQQHQSSNFGGVGER
GGGLRGGKWERTGAFQDTSVATAATAVTENIAGSSLAGAGASASATAART
VPADGGYLAPADFEGPRSRSERRTHGGDTRKYSGFSGGVVDIVSGGDATS
DGREGGGSPPPPPRESPNVAGGLLPASGDRLSGDGGDHSGGGWHEERCWA
DHETGRHRCQANVFLFGVSKCGTTSLASWMAHHPALRWVSNLRMIGRVAE
EAHVLDEMGSEGFQQMLGEGPSGKYGQTAPVAEESDKVIDYTPHCSIKAE
VPYRILDIYGEAARDGSNKYLVQLRDPVARAISSWTSKFDREFLGC*
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mRNA from alignment at E_fasciculatus_S2_contig7657:4166..6865+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E_fasciculatus_S2_contig7657.15825.1 ID=mRNA_E_fasciculatus_S2_contig7657.15825.1|Name=mRNA_E_fasciculatus_S2_contig7657.15825.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=2700bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig7657:4166..6865+ (Ectocarpus fasciculatus EfasUO2)
ATGGCCGCTGGCGCGACCGCTGCGCCAATGCTGCTTGTCTCCCTGCTGTC
TTTCTGGGGGGCCAGGAGAAGCGGCGGCGGGGGCGGTCCTCTCCTCGCCG
GCGCACAGCAACAGCACCAGTCTAGTAACTTCGGGGGGGTCGGTGAACGG
GGGGGGGGCTTGAGAGGCGGAAAATGGGAAAGGACGGGGGCGTTTCAAGA
TACCTCGGTGGCGACGGCGGCGACGGCGGTCACAGAAAACATAGCTGGGA
GTTCTCTTGCTGGTGCTGGGGCTTCCGCTTCCGCGACAGCAGCGCGGACC
GTACCAGCAGACGGGGGCTACTTGGCCCCCGCCGATTTTGAGGGCCCGCG
AAGCCGGTCTGAGCGGCGGACGCATGGAGGCGACACAAGAAAGTACTCGG
GTTTCAGCGGCGGCGTTGTTGATATAGTTAGTGGGGGTGACGCGACAAGC
GACGGTCGGGAAGGGGGGGGGTCACCGCCGCCGCCGCCCAGGGAGAGCCC
TAACGTTGCCGGCGGCTTGCTGCCGGCAAGCGGCGACCGATTGTCGGGCG
ACGGTGGCGATCACTCCGGCGGTGGCTGGCACGAAGAACGCTGCTGGGCT
GACCACGAGACGGGGCGGCACCGGTGTCAGGCCAACGTGTTCCTCTTCGG
CGTGTCCAAGTGCGGTGAGATCATTGTACCGCAGCACTAAGCAGTTGATA
AGAGAGTGCCGGTGGTGGCTATGTCTTCGTTGTTGTTAGGAAATACTTCA
TGCGAACAATCAGAGAAAGACTACCCGTTCGAGACGAAACCTCAGCAACC
ATAGGGATCTTGTTGTTGGCACCGGTAACAAGCGGCGGTGATTCAGTCGC
TCAATTTGGACCGCAGCAACGGGGCACAACGCCCGGGGTTGGGGATGGCA
CTTTCGCGGGGAATGCTAGACGTTTCCGGCAGGTGGCCTCGTAGCAAGGA
AAGCGGGCAGTTTCCTGGTTTTGGTGGCTATCTCTTGCATACGGTTGAAC
GGCAAGTGTCACGACCAAACAGCAAGACAAAATATAGGTTTCGCACTCTT
TCGAACCGGAACTCCTGCACCGAGCACACGACCCCCTTGCTTGCAGGTGG
GAATGTACAGAGGGGCGACTGTACAGAAGGGACTGGTGTGACAGATTCTG
TAGCATCATGTTCATGAAGGAGTTCAGCAGCCAGCCGAAGAATTAGGTGC
AGAAAAGAAATTTAGCTTTCGCGCGAGCTCCTTCGACAACCAAACGGGCG
CCCCCCCCCCCCTCCCCGTGGATTTTCTGCCGATTTGAAGCTTTCGGGGG
AATCACGGCGCCAAGAACCGAGGCGTTCCTAAAGCCCCGCCAACTCCGGA
CGCCCACACAACCATGCCAACCACCATCCCTCTTCTTGAGTTCTCCTCCC
GACGTACGCGGCGCTCGCTGGAGGAGATCGACAAGCTAACCCGCTTCCAC
TCTCTGTCTTGCCCCCTCCGGTGTAAAACCGTCCCGCGAAACGCCGCCGC
CACCGCCACCACCTACACCGGGTTGCGACTTTTGCAGGGACCACTTCTCT
AGCCTCCTGGATGGCCCACCACCCTGCGCTTCGGTGGGTGTCGAACTTGC
GCATGATCGGGCGGGTGGCCGAGGAGGCCCACGTCCTCGACGAGATGGGG
AGTGAGGGCTTCCAGCAGATGCTGGGAGAGGGCCCCTCCGGGAAGTACGG
GCAGACTGCCCCTGTCGCGGAGGAGTCGGACAAGGTCATCGACTGTGAGT
ACAGTACACGACGCGTTGCGTTTTTAATGTGCGGCTCTCCGTACTCGAAC
TTTGCGACGATCACGAGTCTGGGCCCGTGTATCGGTGGGAAACACCCCTC
TAACCCCCCCGCGTCTCATCATGTTCGAAATATGAAATATGTGGTAGCCT
GGGTGTTTAGAACAGGTGTGGGAAGGACAAGGAAAAAACACCCACCTGCG
CCGCGAGGAGGTAACAGTATCGGATGGAGATGGGGACGTCTGTGGCACCG
TGATATTGTAGCCGTCTACAATGGGGTGTTTTTTTTTGTTACGGTGATTG
TTGCTAGATGGGAGACGCCACGCTTGATTTCCCCGGAAGTGGAGCCGGCG
ACGTTTGCGATGGACACAACGGGACAGTGGCATGCGCTATTGCACGGGCA
GAAACTTACACCGACGAACAATGCTACATGTAGCAGCAGCATGCTCAAGA
GACCTCGAAATGTTTGACCCGGAATCTTCGGTAGAGTTAAACATCAACGT
TCTCGATCAAGACGGAGGGAGGAGGACACCGCTCGGTCTCCCACCCAGTT
TTGACGTTTCCTCGTCCCGCTGATTTCCCCTATCGTATTTTCCCACACCT
AGACACCTAGCCTCGCATGTCCTTCGTTGGTTTGGCCGTTCATCATTTCA
CCCTTCATCCCTTCCGTTTAACCCAGGGCCATGATTTACATGTGCCCATT
CCTCGCATTCCGCATCCATTCGTCCTTGTAACGTTGTTTTCCGCTACAAC
GACACGGCTACCTGTGCTGGGGTGCTGCAGACACACCGCACTGCTCCATC
AAGGCGGAGGTTCCCTACAGGATCCTCGACATTTACGGAGAGGCGGCCCG
CGACGGCAGCAACAAGTACCTCGTCCAGCTGCGGGATCCCGTTGCCCGCG
CCATCAGCTCGTGGACGAGCAAGTTCGACCGTGAGTTTTTGGGATGTTAG
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Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig7657:4166..6865+

>mRNA_E_fasciculatus_S2_contig7657.15825.1 ID=mRNA_E_fasciculatus_S2_contig7657.15825.1|Name=mRNA_E_fasciculatus_S2_contig7657.15825.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=1041bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig7657:4166..6865+ (Ectocarpus fasciculatus EfasUO2)
ATGGCCGCTGGCGCGACCGCTGCGCCAATGCTGCTTGTCTCCCTGCTGTC
TTTCTGGGGGGCCAGGAGAAGCGGCGGCGGGGGCGGTCCTCTCCTCGCCG
GCGCACAGCAACAGCACCAGTCTAGTAACTTCGGGGGGGTCGGTGAACGG
GGGGGGGGCTTGAGAGGCGGAAAATGGGAAAGGACGGGGGCGTTTCAAGA
TACCTCGGTGGCGACGGCGGCGACGGCGGTCACAGAAAACATAGCTGGGA
GTTCTCTTGCTGGTGCTGGGGCTTCCGCTTCCGCGACAGCAGCGCGGACC
GTACCAGCAGACGGGGGCTACTTGGCCCCCGCCGATTTTGAGGGCCCGCG
AAGCCGGTCTGAGCGGCGGACGCATGGAGGCGACACAAGAAAGTACTCGG
GTTTCAGCGGCGGCGTTGTTGATATAGTTAGTGGGGGTGACGCGACAAGC
GACGGTCGGGAAGGGGGGGGGTCACCGCCGCCGCCGCCCAGGGAGAGCCC
TAACGTTGCCGGCGGCTTGCTGCCGGCAAGCGGCGACCGATTGTCGGGCG
ACGGTGGCGATCACTCCGGCGGTGGCTGGCACGAAGAACGCTGCTGGGCT
GACCACGAGACGGGGCGGCACCGGTGTCAGGCCAACGTGTTCCTCTTCGG
CGTGTCCAAGTGCGGGACCACTTCTCTAGCCTCCTGGATGGCCCACCACC
CTGCGCTTCGGTGGGTGTCGAACTTGCGCATGATCGGGCGGGTGGCCGAG
GAGGCCCACGTCCTCGACGAGATGGGGAGTGAGGGCTTCCAGCAGATGCT
GGGAGAGGGCCCCTCCGGGAAGTACGGGCAGACTGCCCCTGTCGCGGAGG
AGTCGGACAAGGTCATCGACTACACACCGCACTGCTCCATCAAGGCGGAG
GTTCCCTACAGGATCCTCGACATTTACGGAGAGGCGGCCCGCGACGGCAG
CAACAAGTACCTCGTCCAGCTGCGGGATCCCGTTGCCCGCGCCATCAGCT
CGTGGACGAGCAAGTTCGACCGTGAGTTTTTGGGATGTTAG
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