mRNA_E_fasciculatus_S2_contig7651.15818.1 (mRNA) Ectocarpus fasciculatus EfasUO2

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_E_fasciculatus_S2_contig7651.15818.1
Unique Name	mRNA_E_fasciculatus_S2_contig7651.15818.1
Type	mRNA
Organism	Ectocarpus fasciculatus EfasUO2 (Ectocarpus fasciculatus EfasUO2)

Homology

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BLAST of mRNA_E_fasciculatus_S2_contig7651.15818.1 vs.
Match: DUF6314 (domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FHH0_ECTSI)

HSP 1 Score: 418 bits (1075), Expect = 2.060e-140
Identity = 213/252 (84.52%), Postives = 220/252 (87.30%), Query Frame = 1

Query:  157 MASTTASPITAGTTPTCSRDTSGGVEGSALLGLDTPAGVYRYLRGRWGLEKTIDYKAGGMAGTWRGVATFSPKQQQACDGSNASKEQGEQNHGGDGQDKVDHDSLLFLRYREQGTFRVNGEGAGFEAGQRLVYDCSGPVVRVHFVDDPKKPDALRFFHELDFQTARPCETIAGAGTGGESSXXXXXXXXXXXXXXXXXXRAEFEHLCVRDMYRGKVEVVGPNEFKTSWHVTGPQKDGRINATFKRTLSEEDD 912
            MAST ASPITAGTT TCSR++SG VEGSA LGLDTPAGVYRYLRGRWGLEKTIDYKAGGMAGTWRGVATFSPKQQQACDGSN  KE+GEQNH GDGQD++DH SLLFLRYREQGTFRVNGEGAGFEAGQRLVYDCSGPVVRVHFVDDPKKPDALRFFHELDFQTA P ET AGAGTGGE+                   RAEFEHLCVRDMYRGKVEV GPNEFKTSWHVTGPQKDGRINATFKR LS+EDD
Sbjct:    1 MASTNASPITAGTT-TCSRESSGSVEGSASLGLDTPAGVYRYLRGRWGLEKTIDYKAGGMAGTWRGVATFSPKQQQACDGSNT-KEEGEQNHDGDGQDEMDHSSLLFLRYREQGTFRVNGEGAGFEAGQRLVYDCSGPVVRVHFVDDPKKPDALRFFHELDFQTASPYETTAGAGTGGEAGGTGVAAAGASFAPPPP--RAEFEHLCVRDMYRGKVEVAGPNEFKTSWHVTGPQKDGRINATFKRRLSQEDD 248

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BLAST of mRNA_E_fasciculatus_S2_contig7651.15818.1 vs.
Match: Hypothetical (protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2DLI4_9STRA)

HSP 1 Score: 79.0 bits (193), Expect = 1.260e-13
Identity = 49/129 (37.98%), Postives = 63/129 (48.84%), Query Frame = 1

Query:  259 TPAGVYRYLRGRWGLEKTIDYKAGGMAGTWRGVATFSPKQQQACDGSNASKEQGEQNHGGDGQDKVDHDSLLFLRYREQGTFRVNGEGAGF-EAGQRLVYDCSGPVVRVHFVDDPKKPDALRFFHELDF 642
            TP G++ YL G W L K ++YK GG  G+W GVA+F P       G             GDG  +        L YRE G    +G    F  AG+ L +DCS    RV+F+D P   + LRFFHE+ F
Sbjct:   14 TPMGLHTYLIGSWSLSKRLEYKRGGGFGSWSGVASFIPPNNNLVVGE------------GDGSKQ--------LVYREDGNIIFDGSQKSFTSAGKPLCFDCSVEPTRVYFMDAPD--ERLRFFHEIPF 120

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BLAST of mRNA_E_fasciculatus_S2_contig7651.15818.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KF72_9PHAE)

HSP 1 Score: 70.5 bits (171), Expect = 4.180e-10
Identity = 31/51 (60.78%), Postives = 38/51 (74.51%), Query Frame = 1

Query: 1066 ILRRSPLFAGALARQPDGCLSKRVTFGDIVRGEDPGRWCPEQRWAKCFKDD 1218
            + +R  LFAGA+ARQPDG L KR+ FG++V GE+PGR  PEQ W  C KDD
Sbjct:   55 VRQRCLLFAGAMARQPDGRLPKRLMFGELVGGENPGRGSPEQNWLICLKDD 105

[Close]

BLAST of mRNA_E_fasciculatus_S2_contig7651.15818.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JS41_9PHAE)

HSP 1 Score: 66.6 bits (161), Expect = 8.480e-10
Identity = 29/45 (64.44%), Postives = 34/45 (75.56%), Query Frame = 1

Query: 1084 LFAGALARQPDGCLSKRVTFGDIVRGEDPGRWCPEQRWAKCFKDD 1218
            L AGA+ARQPDG L KR+ FG++V GE+PGR  PEQ W  C KDD
Sbjct:    2 LVAGAVARQPDGRLPKRLMFGELVGGENPGRGSPEQNWLICLKDD 46

[Close]

BLAST of mRNA_E_fasciculatus_S2_contig7651.15818.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KLX9_9PHAE)

HSP 1 Score: 71.2 bits (173), Expect = 1.000e-9
Identity = 35/74 (47.30%), Postives = 46/74 (62.16%), Query Frame = 1

Query: 1066 ILRRSPLFAGALARQPDGCLSKRVTFGDIVRGEDPGRWCPEQRWAKCFKDDSSKSSKIQQFVVVDSQGGSRLNT 1287
            + +R  LFAGA+ARQPDG L KR+ FG++V GEDPG+  PEQ W  C KDD      ++ F   + +G   L T
Sbjct:  225 VRQRRLLFAGAVARQPDGRLPKRLMFGELVGGEDPGQGNPEQNWLTCLKDD------MKMFEARELRGSWPLGT 292

[Close]

BLAST of mRNA_E_fasciculatus_S2_contig7651.15818.1 vs.
Match: Uncharacterized (protein n=2 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JLH5_9PHAE)

HSP 1 Score: 70.5 bits (171), Expect = 1.030e-9
Identity = 31/51 (60.78%), Postives = 38/51 (74.51%), Query Frame = 1

Query: 1066 ILRRSPLFAGALARQPDGCLSKRVTFGDIVRGEDPGRWCPEQRWAKCFKDD 1218
            + +R  LFAGA+ARQPDG L KR+ FG++V GEDPG+  PEQ W  C KDD
Sbjct:  174 VRQRRLLFAGAVARQPDGRLPKRLMFGELVGGEDPGQGNPEQNWLTCLKDD 224

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BLAST of mRNA_E_fasciculatus_S2_contig7651.15818.1 vs.
Match: Reverse (transcriptase domain-containing protein n=2 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JWS4_9PHAE)

HSP 1 Score: 70.5 bits (171), Expect = 2.450e-9
Identity = 31/51 (60.78%), Postives = 38/51 (74.51%), Query Frame = 1

Query: 1066 ILRRSPLFAGALARQPDGCLSKRVTFGDIVRGEDPGRWCPEQRWAKCFKDD 1218
            + +R  LFAGA+ARQPDG L KR+ FG++V GEDPG+  PEQ W  C KDD
Sbjct:  193 VRQRRLLFAGAVARQPDGRLPKRLMFGELVGGEDPGQGNPEQNWLTCLKDD 243

[Close]

BLAST of mRNA_E_fasciculatus_S2_contig7651.15818.1 vs.
Match: Reverse (transcriptase domain-containing protein n=9 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K7U5_9PHAE)

HSP 1 Score: 72.0 bits (175), Expect = 2.790e-9
Identity = 32/51 (62.75%), Postives = 38/51 (74.51%), Query Frame = 1

Query: 1066 ILRRSPLFAGALARQPDGCLSKRVTFGDIVRGEDPGRWCPEQRWAKCFKDD 1218
            + +R  LFAGA+ARQPDG L KR+ FG++V GEDPGR  PEQ W  C KDD
Sbjct:  973 VRQRRLLFAGAVARQPDGRLPKRLMFGELVGGEDPGRGNPEQNWLTCLKDD 1023

[Close]

BLAST of mRNA_E_fasciculatus_S2_contig7651.15818.1 vs.
Match: Reverse (transcriptase domain-containing protein n=5 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KLC4_9PHAE)

HSP 1 Score: 70.5 bits (171), Expect = 4.510e-9
Identity = 31/51 (60.78%), Postives = 38/51 (74.51%), Query Frame = 1

Query: 1066 ILRRSPLFAGALARQPDGCLSKRVTFGDIVRGEDPGRWCPEQRWAKCFKDD 1218
            + +R  LFAGA+ARQPDG L KR+ FG++V GEDPG+  PEQ W  C KDD
Sbjct:  315 VRQRRLLFAGAVARQPDGRLPKRLMFGELVGGEDPGQGNPEQNWLTCLKDD 365

[Close]

BLAST of mRNA_E_fasciculatus_S2_contig7651.15818.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K9G5_9PHAE)

HSP 1 Score: 70.5 bits (171), Expect = 6.360e-9
Identity = 31/51 (60.78%), Postives = 38/51 (74.51%), Query Frame = 1

Query: 1066 ILRRSPLFAGALARQPDGCLSKRVTFGDIVRGEDPGRWCPEQRWAKCFKDD 1218
            + +R  LFAGA+ARQPDG L KR+ FG++V GEDPG+  PEQ W  C KDD
Sbjct:  499 VRQRRLLFAGAVARQPDGRLPKRLMFGELVGGEDPGQGNPEQNWLTCLKDD 549

The following BLAST results are available for this feature:

BLAST of mRNA_E_fasciculatus_S2_contig7651.15818.1 vs.

BLAST of mRNA_E_fasciculatus_S2_contig7651.15818.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90)
Total hits: 25


Match Name	E-value	Identity	Description
DUF6314	2.060e-140	84.52	domain-containing protein n=2 Tax=Ectocarpus TaxID...	[more]
Hypothetical	1.260e-13	37.98	protein n=1 Tax=Dictyocha speculum TaxID=35687 Rep...	[more]
Uncharacterized	4.180e-10	60.78	protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=...	[more]
Uncharacterized	8.480e-10	64.44	protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=...	[more]
Uncharacterized	1.000e-9	47.30	protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=...	[more]
Uncharacterized	1.030e-9	60.78	protein n=2 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=...	[more]
Reverse	2.450e-9	60.78	transcriptase domain-containing protein n=2 Tax=Ec...	[more]
Reverse	2.790e-9	62.75	transcriptase domain-containing protein n=9 Tax=Ec...	[more]
Reverse	4.510e-9	60.78	transcriptase domain-containing protein n=5 Tax=Ec...	[more]
Uncharacterized	6.360e-9	60.78	protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=...	[more]

Pages

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
E_fasciculatus_S2_contig7651	contig	E_fasciculatus_S2_contig7651:38..2485 -

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus EfasUO2 vs UniRef90	2022-09-19
OGS1.0 of Ectocarpus fasciculatus EfasUO2	2022-07-07

Properties


Property Name	Value
Taxonomic scope	Eukaryota
Stop	1
Start	1
Seed ortholog score	420.2
Seed ortholog evalue	8e-115
Seed eggNOG ortholog	2880.D7FHH0
Model size	1962
Hectar predicted targeting category	signal peptide
Exons	2
EggNOG OGs	2E4H1@1,2SG7J@2759
Ec32 ortholog description	expressed unknown protein
Ec32 ortholog	Ec-25_002440.1
Cds size	915
Best tax level	Eukaryota
Best eggNOG OG	NA\|NA\|NA

Relationships

The following UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1696842427.3244147-UTR-E_fasciculatus_S2_contig7651:37..1084	1696842427.3244147-UTR-E_fasciculatus_S2_contig7651:37..1084	Ectocarpus fasciculatus EfasUO2	UTR	E_fasciculatus_S2_contig7651 38..1084 -

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1696842427.3347945-CDS-E_fasciculatus_S2_contig7651:1084..1163	1696842427.3347945-CDS-E_fasciculatus_S2_contig7651:1084..1163	Ectocarpus fasciculatus EfasUO2	CDS	E_fasciculatus_S2_contig7651 1085..1163 -
1696842427.3444881-CDS-E_fasciculatus_S2_contig7651:1649..2485	1696842427.3444881-CDS-E_fasciculatus_S2_contig7651:1649..2485	Ectocarpus fasciculatus EfasUO2	CDS	E_fasciculatus_S2_contig7651 1650..2485 -

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_E_fasciculatus_S2_contig7651.15818.1	prot_E_fasciculatus_S2_contig7651.15818.1	Ectocarpus fasciculatus EfasUO2	polypeptide	E_fasciculatus_S2_contig7651 1085..2485 -

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at E_fasciculatus_S2_contig7651:38..2485-
coding sequence from alignment at E_fasciculatus_S2_contig7651:38..2485-

protein sequence of mRNA_E_fasciculatus_S2_contig7651.15818.1

>prot_E_fasciculatus_S2_contig7651.15818.1 ID=prot_E_fasciculatus_S2_contig7651.15818.1|Name=mRNA_E_fasciculatus_S2_contig7651.15818.1|organism=Ectocarpus fasciculatus EfasUO2|type=polypeptide|length=305bp
MPPVRLLALTVCGCLSSALRRPVRPQLLHRCHHHQHQYPTSSSAVRGLRF
KSMASTTASPITAGTTPTCSRDTSGGVEGSALLGLDTPAGVYRYLRGRWG
LEKTIDYKAGGMAGTWRGVATFSPKQQQACDGSNASKEQGEQNHGGDGQD
KVDHDSLLFLRYREQGTFRVNGEGAGFEAGQRLVYDCSGPVVRVHFVDDP
KKPDALRFFHELDFQTARPCETIAGAGTGGESSGTGVAAAAGASFAPPPP
PRAEFEHLCVRDMYRGKVEVVGPNEFKTSWHVTGPQKDGRINATFKRTLS
EEDD*

mRNA from alignment at E_fasciculatus_S2_contig7651:38..2485-

Legend: polypeptideCDSUTR

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_E_fasciculatus_S2_contig7651.15818.1 ID=mRNA_E_fasciculatus_S2_contig7651.15818.1|Name=mRNA_E_fasciculatus_S2_contig7651.15818.1|organism=Ectocarpus fasciculatus EfasUO2|type=mRNA|length=2448bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig7651:38..2485- (Ectocarpus fasciculatus EfasUO2)
ATGCCCCCTGTGAGACTGCTAGCACTGACTGTCTGCGGCTGCCTTTCCTC
TGCTCTACGGAGACCTGTACGTCCCCAGCTGCTGCACCGCTGCCACCATC
ACCAACACCAATATCCGACCTCATCATCAGCGGTTCGCGGCCTTCGTTTC
AAGAGCATGGCGTCAACAACGGCGAGCCCTATCACTGCTGGAACAACACC
AACATGCAGCAGAGATACTAGTGGCGGCGTCGAAGGATCCGCTTTGCTCG
GCCTCGACACCCCTGCCGGGGTGTATCGGTATCTCCGTGGTCGATGGGGG
CTCGAGAAGACTATCGACTACAAGGCCGGGGGAATGGCGGGGACCTGGAG
AGGAGTCGCAACATTCTCTCCCAAACAACAGCAAGCTTGCGACGGCAGCA
ACGCCAGCAAGGAGCAGGGAGAACAAAACCACGGTGGTGATGGCCAAGAC
AAGGTGGACCACGACTCTCTGCTCTTTCTGCGCTACAGGGAACAGGGAAC
CTTTCGAGTGAACGGCGAGGGTGCCGGCTTCGAAGCTGGGCAGCGGCTGG
TCTACGACTGCAGCGGCCCCGTCGTGCGCGTTCACTTCGTCGACGACCCG
AAAAAGCCCGATGCTCTCCGATTTTTTCACGAACTCGACTTTCAAACAGC
ACGCCCGTGCGAGACAATAGCAGGGGCTGGAACCGGCGGGGAGTCAAGCG
GTACGGGGGTGGCGGCGGCAGCGGGGGCTAGTTTTGCGCCGCCGCCGCCG
CCGCGAGCGGAGTTTGAGCACCTGTGTGTCCGAGACATGTACAGGGGGAA
GGTGGAGGTGGTCGGGCCAAACGAGTTCAAGACAAGGTATGCAATAACCG
TGGTTGCTCCACGTCAACTTGCTCGTTTTTTTGGATACTTCTAGTGAGGT
TAGCGGTGTACATAATCACAACGCTGCCTGGAAAGGCCTTCCTGTGTTGT
TCAGAACCTTGCCACATGTGGATTTGAAGTGAAGGTGTAAGCTTTGAGAT
TCTCGTTATACCACGTACCGGAACCAGCTAGCAGTACCAGGTGAAGTATT
TTCCTTTCATGTGGTTACAGGTGTCCATCGATGGGAAGTGGAAGTCTCGG
CGTGTGTAGGCTGTGTCTATTGCATCGTCTGCAAGACGTGAAACCGGCGA
CGTGCTTAGTTGAATTAAAACCCTCTGCTCCTTTGCGCCAGCAATGGCAT
TTGGCGAAGCCGCCGGTAGGAACCTCTATTCTGAGGGTTATTATTCCACA
CTGCACCTCCCACAGTGCTGCCGCACTCCCAACAATGCCCTCGTTGTGTT
CACCGCAACTCTCTCGTCACAGTTGGCACGTTACGGGGCCGCAGAAGGAT
GGCCGCATAAACGCCACCTTTAAACGAACACTATCCGAGGAAGACGATTG
ACAAGACCGTTCCGTGGCTGGGCCTACTGCCAGCCTTGGTTAGTTTGAGC
AACCATGCAAGTTTCCTACGTGCAGCCGCATGAGCGCCGAAGATATAGGT
CCTTCGCCCAGACGAAGCTAGCTACCGAAGCGTGGACACCACTCCGTCCG
GATACTGCGGCGATCACCGCTGTTTGCTGGAGCATTAGCCAGACAACCGG
ACGGATGTCTCTCGAAAAGAGTGACGTTCGGCGACATCGTGAGGGGGGAG
GACCCAGGCAGGTGGTGCCCGGAACAGCGTTGGGCGAAATGCTTCAAGGA
CGATTCTTCCAAGAGTTCGAAGATTCAGCAGTTCGTTGTCGTTGATTCAC
AGGGAGGTAGTAGACTTAACACTCCGTATTGTGAGGTAGACAAGAGAGAG
CTGGCTGCGCGTATGCGCACAGTACATGCCAACAACGGAGTCACTGCGAC
ATAGTGTAGTAGAACGATCGTTTGTGTGAAGCATGCATTGGTGGTAGTCT
TTGGTGCGGCCGGTATGACTTTGCTGCCATCGCATAGGGGGCACCCGCGA
TTGCGGGGTGCACATGTGATGGGAGGAAAGGTAGTTGGATCAATAACTAA
TTTCCAGTCGGCAAACAATTATCTCCAAGAAGATTATCTTCGTGGATGAA
TGCCCGCACTCAATTCCAACCGCTTAGGCCGAGTCTTACGGTCTGATTTT
TTCTTGGATTGTTTTTTATCCTCTCTCGGGGGGTGGTGGTCTGCGCAGGA
AAAATTATCCAGATCCAATCGACCTTCTACGGTTCCTCGACGGATTTTGT
TTTTCCAAGGGGAAAACATAGGCCGATTCAGGTTTGTGTTGGTCACAACA
TGCTTCAGTTCACTTTTTCGGCTCGTGCTCGACCAACAAGACACGGAGCG
CCCTGTTCTTGGGCCGCCTGCAGAGGGCATGTGTATGATCGCACGCCATT
GGCCGCAGTTTGTAGATATCGTCGGCCGAAGGCCTCTTTCTGGCATGCGT
TGTGGACTGATCACACTCGGTGCAACCAGAAAAAAAAAGGCAACGTCC

Coding sequence (CDS) from alignment at E_fasciculatus_S2_contig7651:38..2485-

>mRNA_E_fasciculatus_S2_contig7651.15818.1 ID=mRNA_E_fasciculatus_S2_contig7651.15818.1|Name=mRNA_E_fasciculatus_S2_contig7651.15818.1|organism=Ectocarpus fasciculatus EfasUO2|type=CDS|length=915bp|location=Sequence derived from alignment at E_fasciculatus_S2_contig7651:38..2485- (Ectocarpus fasciculatus EfasUO2)
ATGCCCCCTGTGAGACTGCTAGCACTGACTGTCTGCGGCTGCCTTTCCTC
TGCTCTACGGAGACCTGTACGTCCCCAGCTGCTGCACCGCTGCCACCATC
ACCAACACCAATATCCGACCTCATCATCAGCGGTTCGCGGCCTTCGTTTC
AAGAGCATGGCGTCAACAACGGCGAGCCCTATCACTGCTGGAACAACACC
AACATGCAGCAGAGATACTAGTGGCGGCGTCGAAGGATCCGCTTTGCTCG
GCCTCGACACCCCTGCCGGGGTGTATCGGTATCTCCGTGGTCGATGGGGG
CTCGAGAAGACTATCGACTACAAGGCCGGGGGAATGGCGGGGACCTGGAG
AGGAGTCGCAACATTCTCTCCCAAACAACAGCAAGCTTGCGACGGCAGCA
ACGCCAGCAAGGAGCAGGGAGAACAAAACCACGGTGGTGATGGCCAAGAC
AAGGTGGACCACGACTCTCTGCTCTTTCTGCGCTACAGGGAACAGGGAAC
CTTTCGAGTGAACGGCGAGGGTGCCGGCTTCGAAGCTGGGCAGCGGCTGG
TCTACGACTGCAGCGGCCCCGTCGTGCGCGTTCACTTCGTCGACGACCCG
AAAAAGCCCGATGCTCTCCGATTTTTTCACGAACTCGACTTTCAAACAGC
ACGCCCGTGCGAGACAATAGCAGGGGCTGGAACCGGCGGGGAGTCAAGCG
GTACGGGGGTGGCGGCGGCAGCGGGGGCTAGTTTTGCGCCGCCGCCGCCG
CCGCGAGCGGAGTTTGAGCACCTGTGTGTCCGAGACATGTACAGGGGGAA
GGTGGAGGTGGTCGGGCCAAACGAGTTCAAGACAAGTTGGCACGTTACGG
GGCCGCAGAAGGATGGCCGCATAAACGCCACCTTTAAACGAACACTATCC
GAGGAAGACGATTGA