mRNA_E-fasciculatus_F_contig16043.4138.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig16043.4138.1
Unique NamemRNA_E-fasciculatus_F_contig16043.4138.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig16043.4138.1 vs. uniprot
Match: D8LFH3_ECTSI (Glucosylceramidase, family GH30 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LFH3_ECTSI)

HSP 1 Score: 209 bits (533), Expect = 2.740e-61
Identity = 99/104 (95.19%), Postives = 103/104 (99.04%), Query Frame = 1 
Query:   76 AVCAERGVSGTVVVESDEKLQEIIGFGGAFTDAATINLFKLPEDVQEKVMDAYFGPNGIEYSVGRIPMGSCDFSVEQYSFDEVPGDYNLTHFDDGVEKDTAQRV 387
            AVCAERGVSGTVVVESD+KLQEIIGFGGAFTDAATIN FKLPEDVQE+V+DAYFGPNGIEYSVGRIPMGSCDFSVEQYSFDEVPGDYNLTHFDDGVEKDTAQR+
Sbjct:  126 AVCAERGVSGTVVVESDDKLQEIIGFGGAFTDAATINFFKLPEDVQEQVLDAYFGPNGIEYSVGRIPMGSCDFSVEQYSFDEVPGDYNLTHFDDGVEKDTAQRI 229
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BLAST of mRNA_E-fasciculatus_F_contig16043.4138.1 vs. uniprot
Match: A0A835Z8L7_9STRA (Glucosylceramidase, family GH30 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z8L7_9STRA)

HSP 1 Score: 139 bits (351), Expect = 5.630e-36
Identity = 60/95 (63.16%), Postives = 79/95 (83.16%), Query Frame = 1 
Query:  103 GTVVVESDEKLQEIIGFGGAFTDAATINLFKLPEDVQEKVMDAYFGPNGIEYSVGRIPMGSCDFSVEQYSFDEVPGDYNLTHFDDGVEKDTAQRV 387
            G + V+S    QEI+GFG AFTD+ATIN +KLP++ Q+ V+DAYFGP+GI Y++GRIPMGSCDFSVEQY++D+VPGDY++ HFD+ +E D  QRV
Sbjct:   30 GRIDVDSTASYQEIVGFGAAFTDSATINFYKLPKEAQDFVIDAYFGPDGIGYTIGRIPMGSCDFSVEQYNYDDVPGDYSMEHFDNNIEYDQKQRV 124
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BLAST of mRNA_E-fasciculatus_F_contig16043.4138.1 vs. uniprot
Match: UPI0006B08D0A (glucosylceramidase-like isoform X1 n=2 Tax=Limulus polyphemus TaxID=6850 RepID=UPI0006B08D0A)

HSP 1 Score: 115 bits (287), Expect = 2.530e-27
Identity = 46/81 (56.79%), Postives = 65/81 (80.25%), Query Frame = 1 
Query:  109 VVVESDEKLQEIIGFGGAFTDAATINLFKLPEDVQEKVMDAYFGPNGIEYSVGRIPMGSCDFSVEQYSFDEVPGDYNLTHF 351
            V V   +K QEI+GFGGAFTDAA IN+  LP+ +Q+ ++ +YF P+G+EYS+GR+P+ SCDFS   YS+D++PGD+NLT+F
Sbjct:  103 VTVNKSQKFQEILGFGGAFTDAAGINIVSLPKKMQDMLISSYFSPDGLEYSIGRVPIASCDFSTHDYSYDDIPGDFNLTYF 183
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BLAST of mRNA_E-fasciculatus_F_contig16043.4138.1 vs. uniprot
Match: UPI001425A5C3 (lysosomal acid glucosylceramidase-like n=1 Tax=Anneissia japonica TaxID=1529436 RepID=UPI001425A5C3)

HSP 1 Score: 114 bits (286), Expect = 3.780e-27
Identity = 48/81 (59.26%), Postives = 65/81 (80.25%), Query Frame = 1 
Query:  109 VVVESDEKLQEIIGFGGAFTDAATINLFKLPEDVQEKVMDAYFGPNGIEYSVGRIPMGSCDFSVEQYSFDEVPGDYNLTHF 351
            + +    + Q+++GFGGAFTD+ATIN+ KL E VQE V+++YF  NGIEY++GRIPM SCDFS   YS+D+VPGD+NLT+F
Sbjct:   89 LTINRTARYQKVLGFGGAFTDSATINIMKLSESVQEHVLNSYFSKNGIEYNMGRIPMASCDFSTRNYSYDDVPGDFNLTNF 169
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BLAST of mRNA_E-fasciculatus_F_contig16043.4138.1 vs. uniprot
Match: A0A6B0VE65_IXORI (Glucosylceramidase (Fragment) n=1 Tax=Ixodes ricinus TaxID=34613 RepID=A0A6B0VE65_IXORI)

HSP 1 Score: 113 bits (282), Expect = 7.090e-27
Identity = 48/88 (54.55%), Postives = 68/88 (77.27%), Query Frame = 1 
Query:   88 ERGVSGTVVVESDEKLQEIIGFGGAFTDAATINLFKLPEDVQEKVMDAYFGPNGIEYSVGRIPMGSCDFSVEQYSFDEVPGDYNLTHF 351
            E   S +VV++S +K Q I GFGGAFTDAA INL  LPE++QE ++ +Y+   G+ Y++GR+PM SCDFSV QY++++VPGD ++THF
Sbjct:   14 ENADSVSVVIDSSKKYQSIFGFGGAFTDAAGINLQSLPEELQEDILKSYYSNEGLSYNIGRVPMASCDFSVRQYTYNDVPGDLDMTHF 101
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BLAST of mRNA_E-fasciculatus_F_contig16043.4138.1 vs. uniprot
Match: UPI000C6EA664 (putative glucosylceramidase 4 n=1 Tax=Centruroides sculpturatus TaxID=218467 RepID=UPI000C6EA664)

HSP 1 Score: 107 bits (266), Expect = 2.140e-26
Identity = 44/93 (47.31%), Postives = 69/93 (74.19%), Query Frame = 1 
Query:  109 VVVESDEKLQEIIGFGGAFTDAATINLFKLPEDVQEKVMDAYFGPNGIEYSVGRIPMGSCDFSVEQYSFDEVPGDYNLTHFDDGVEKDTAQRV 387
            + ++  ++ QEI+GFGGAFTDAA IN+  LPE +Q++++  Y+  +G++Y+ GR+P+ SCDFS   YS+D+VPGD NLTHF    E+D+  ++
Sbjct:   11 ISIDRTKRYQEILGFGGAFTDAAGINILSLPEQLQDQILRGYYSSDGLDYTFGRVPIASCDFSTHVYSYDDVPGDANLTHFS-LTEEDSKLKI 102
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BLAST of mRNA_E-fasciculatus_F_contig16043.4138.1 vs. uniprot
Match: A0A7S2V2A4_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V2A4_9STRA)

HSP 1 Score: 110 bits (274), Expect = 2.250e-25
Identity = 48/95 (50.53%), Postives = 68/95 (71.58%), Query Frame = 1 
Query:  103 GTVVVESDEKLQEIIGFGGAFTDAATINLFKLPEDVQEKVMDAYFGPNGIEYSVGRIPMGSCDFSVEQYSFDEVPGDYNLTHFDDGVEKDTAQRV 387
            G V V++ +K QE++GFGGAFT+++  N   LP+ +QEKV++AYFG +GI YSVGR+ + SCDFS+  Y+FD+V  D+ L HFD  V  D A  +
Sbjct:  118 GFVEVDTSQKFQEVMGFGGAFTESSAFNWAMLPKKIQEKVLEAYFGEDGIGYSVGRVHINSCDFSMASYNFDDVEDDFELEHFDSNVTHDAALLI 212
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BLAST of mRNA_E-fasciculatus_F_contig16043.4138.1 vs. uniprot
Match: UPI0003F0CE10 (glucosylceramidase-like n=1 Tax=Saccoglossus kowalevskii TaxID=10224 RepID=UPI0003F0CE10)

HSP 1 Score: 108 bits (269), Expect = 3.490e-25
Identity = 45/82 (54.88%), Postives = 64/82 (78.05%), Query Frame = 1 
Query:  106 TVVVESDEKLQEIIGFGGAFTDAATINLFKLPEDVQEKVMDAYFGPNGIEYSVGRIPMGSCDFSVEQYSFDEVPGDYNLTHF 351
            T  + S    Q+I+GFGGAFTDAA IN+  L +DVQ++++++Y+ PNGIEY++GRIPM SCDFS   YS+D+V GD++L +F
Sbjct:   25 TFTINSTHTYQQILGFGGAFTDAAGINILNLTKDVQDRLLESYYSPNGIEYTLGRIPMASCDFSTHPYSYDDVVGDFSLENF 106
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BLAST of mRNA_E-fasciculatus_F_contig16043.4138.1 vs. uniprot
Match: A0A6P7V3Q0_IXOSC (Glucosylceramidase n=6 Tax=Ixodes TaxID=6944 RepID=A0A6P7V3Q0_IXOSC)

HSP 1 Score: 108 bits (271), Expect = 3.990e-25
Identity = 45/82 (54.88%), Postives = 65/82 (79.27%), Query Frame = 1 
Query:  106 TVVVESDEKLQEIIGFGGAFTDAATINLFKLPEDVQEKVMDAYFGPNGIEYSVGRIPMGSCDFSVEQYSFDEVPGDYNLTHF 351
            +VV++S +K Q I GFGGAFTDAA INL  LPE++QE ++ +Y+   G+ Y++GR+PM SCDFSV  Y++++VPGD ++THF
Sbjct:   90 SVVIDSSKKYQSIFGFGGAFTDAAGINLQSLPEELQEDLLKSYYSVEGLSYNIGRVPMASCDFSVRLYTYNDVPGDLDMTHF 171
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BLAST of mRNA_E-fasciculatus_F_contig16043.4138.1 vs. uniprot
Match: V5GP52_IXORI (Glucosylceramidase (Fragment) n=2 Tax=Ixodes ricinus TaxID=34613 RepID=V5GP52_IXORI)

HSP 1 Score: 102 bits (255), Expect = 5.230e-25
Identity = 43/82 (52.44%), Postives = 62/82 (75.61%), Query Frame = 1 
Query:  106 TVVVESDEKLQEIIGFGGAFTDAATINLFKLPEDVQEKVMDAYFGPNGIEYSVGRIPMGSCDFSVEQYSFDEVPGDYNLTHF 351
            +VV++S +K Q I GFGGAFTDAA IN+  LPE +QE V+ +Y+   G+ Y++GR+PM SCDFSV QY++++ PG  N+ +F
Sbjct:   89 SVVIDSSKKYQSIFGFGGAFTDAAGINIQSLPEQLQEGVLKSYYSDEGLSYNMGRVPMASCDFSVRQYTYNDAPGYLNMANF 170
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig16043.4138.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LFH3_ECTSI2.740e-6195.19Glucosylceramidase, family GH30 n=1 Tax=Ectocarpus... [more]
A0A835Z8L7_9STRA5.630e-3663.16Glucosylceramidase, family GH30 n=1 Tax=Tribonema ... [more]
UPI0006B08D0A2.530e-2756.79glucosylceramidase-like isoform X1 n=2 Tax=Limulus... [more]
UPI001425A5C33.780e-2759.26lysosomal acid glucosylceramidase-like n=1 Tax=Ann... [more]
A0A6B0VE65_IXORI7.090e-2754.55Glucosylceramidase (Fragment) n=1 Tax=Ixodes ricin... [more]
UPI000C6EA6642.140e-2647.31putative glucosylceramidase 4 n=1 Tax=Centruroides... [more]
A0A7S2V2A4_9STRA2.250e-2550.53Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
UPI0003F0CE103.490e-2554.88glucosylceramidase-like n=1 Tax=Saccoglossus kowal... [more]
A0A6P7V3Q0_IXOSC3.990e-2554.88Glucosylceramidase n=6 Tax=Ixodes TaxID=6944 RepID... [more]
V5GP52_IXORI5.230e-2552.44Glucosylceramidase (Fragment) n=2 Tax=Ixodes ricin... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig16043contigE-fasciculatus_F_contig16043:41..1284 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score210.7
Seed ortholog evalue4.3e-52
Seed eggNOG ortholog2880.D8LFH3
KEGG rclassRC00059,RC00451
KEGG koko:K01201
KEGG ReactionR01498
KEGG Pathwayko00511,ko00600,ko01100,ko04142,map00511,map00600,map01100,map04142
GOsGO:0000323,GO:0003008,GO:0003674,GO:0003824,GO:0004348,GO:0004553,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005773,GO:0006629,GO:0006643,GO:0006664,GO:0006665,GO:0006672,GO:0006677,GO:0006678,GO:0006680,GO:0006687,GO:0006807,GO:0006950,GO:0006986,GO:0007275,GO:0007568,GO:0007610,GO:0007611,GO:0007613,GO:0007626,GO:0008150,GO:0008152,GO:0008340,GO:0008344,GO:0009056,GO:0009987,GO:0010033,GO:0010259,GO:0016042,GO:0016787,GO:0016798,GO:0019377,GO:0019725,GO:0030149,GO:0030534,GO:0032501,GO:0032502,GO:0033554,GO:0034620,GO:0034641,GO:0035966,GO:0035967,GO:0042221,GO:0042592,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043603,GO:0044237,GO:0044238,GO:0044242,GO:0044248,GO:0044255,GO:0044424,GO:0044444,GO:0044464,GO:0046466,GO:0046477,GO:0046479,GO:0046514,GO:0048856,GO:0050877,GO:0050890,GO:0050896,GO:0051716,GO:0060249,GO:0065007,GO:0065008,GO:0070050,GO:0070887,GO:0071310,GO:0071704,GO:1901135,GO:1901136,GO:1901564,GO:1901565,GO:1901575,GO:1903509
EggNOG free text desc.glucosylceramidase activity
EggNOG OGsCOG5520@1,KOG2566@2759
EC3.2.1.45
COG Functional cat.M
CAZyGH30
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Hectar predicted targeting categoryother localisation
Exons2
Model size405
Cds size405
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928072.4059448-CDS-E-fasciculatus_F_contig16043:40..2261622928072.4059448-CDS-E-fasciculatus_F_contig16043:40..226Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig16043 41..226 -
1695995811.8625906-CDS-E-fasciculatus_F_contig16043:40..2261695995811.8625906-CDS-E-fasciculatus_F_contig16043:40..226Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig16043 41..226 -
1622928072.416488-CDS-E-fasciculatus_F_contig16043:1065..12841622928072.416488-CDS-E-fasciculatus_F_contig16043:1065..1284Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig16043 1066..1284 -
1695995811.886781-CDS-E-fasciculatus_F_contig16043:1065..12841695995811.886781-CDS-E-fasciculatus_F_contig16043:1065..1284Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig16043 1066..1284 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig16043.4138.1prot_E-fasciculatus_F_contig16043.4138.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig16043 41..1284 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig16043.4138.1

>prot_E-fasciculatus_F_contig16043.4138.1 ID=prot_E-fasciculatus_F_contig16043.4138.1|Name=mRNA_E-fasciculatus_F_contig16043.4138.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=135bp
SSSSSSSDDDDGSGSSDSDEEEEATAVCAERGVSGTVVVESDEKLQEIIG
FGGAFTDAATINLFKLPEDVQEKVMDAYFGPNGIEYSVGRIPMGSCDFSV
EQYSFDEVPGDYNLTHFDDGVEKDTAQRVGGWGE*
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mRNA from alignment at E-fasciculatus_F_contig16043:41..1284-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig16043.4138.1 ID=mRNA_E-fasciculatus_F_contig16043.4138.1|Name=mRNA_E-fasciculatus_F_contig16043.4138.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=1244bp|location=Sequence derived from alignment at E-fasciculatus_F_contig16043:41..1284- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
AGCAGCAGCAGCAGCAGCTCCGACGACGATGATGGCAGTGGCAGCAGCGA
CAGCGATGAGGAGGAGGAGGCTACAGCGGTCTGCGCAGAGAGGGGTGTGT
CGGGGACGGTGGTGGTGGAGTCGGACGAGAAGCTGCAGGAGATTATCGGA
TTCGGGGGGGCTTTCACCGACGCCGCCACGATCAACTTGTTCAAGCTGCC
GGAGGATGTTCAGGAGAAGGTGATGGGGTGCCTGCCTCCCTGCGGTGCTG
CTGCTTGGTTTTTGGCTGCACGCTGCGGTGTTGTGTTTTCTCGCGCTTAT
GAAAACGCAAACTATTATCTATAATATGAGCGATAAGTTGTGAGGTGTCC
GGCAGTTTCTTTTCTCAGCACTCGCTGTGAAGACACCTCTTGGACTGAAG
TACTGTATTATGTCGAACGGGTACAGCGGTCGTCCCTCGTTCAATGGCAC
ACATGGTCTTTCGACGAAACAGGTGAATATCCTTAAGCCGATGGAGTGTC
CATAAGGTTGATACCCTCCTTCCTTGAGGGACACCTGCCTATGACAAAGA
CCGGGTCCTTACCCGGGGGAGTGTCCTTCAAAATGGTGTCCTCGACTGAG
GGACGACTGTATTCTTTCTGTGATTGTATTTTCTACTTGTATACACTTAT
GAAGAATATGGTGATGGTAGAACGCATGATGACGTCACCCACCCCTTCTG
ATGATGCTTGGAATTAGTAGATCCGGCGTCCACACTTGCGTTTTTATCTC
GCGCCCATCAACGCAGACCTTTGGCAAAGTAGGAAGGAACGATTGGCCAT
CGGCGATCGGTCCTGTGGGAACAATGCTCCGTCCCCTCGAGGACGCGTGG
TCAATGGTCTAACGGCTAAGGCATGCAAAACATGAGAGTACGCCCCCCCC
CCCGCCCCCTCCATCAACGCAAGCAAGAAGAGGATTGTAGACCGATGATG
GCTTTCACTCCTTTCCGTTCGCTTGGAATTGGTAGGTTTAACATCTACAC
TCTTGTTCTTTCTCGCGCTCTTGATGGTGATGGTGATGCAATGTTGTCTA
ACCGACAGGTGATGGACGCGTACTTCGGACCCAACGGCATCGAGTACAGC
GTCGGCCGCATCCCCATGGGCAGCTGCGACTTCAGCGTGGAGCAGTACAG
CTTCGACGAAGTACCGGGAGACTACAATCTCACGCACTTCGACGATGGCG
TGGAGAAGGACACCGCTCAGAGGGTAGGGGGGTGGGGAGAATAG
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig16043:41..1284-

>mRNA_E-fasciculatus_F_contig16043.4138.1 ID=mRNA_E-fasciculatus_F_contig16043.4138.1|Name=mRNA_E-fasciculatus_F_contig16043.4138.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=810bp|location=Sequence derived from alignment at E-fasciculatus_F_contig16043:41..1284- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
AGCAGCAGCAGCAGCAGCTCCGACGACGATGATGGCAGTGGCAGCAGCGA
CAGCGATGAGGAGGAGGAGGCTACAGCGGTCTGCGCAGAGAGGGGTGTGT
CGGGGACGGTGGTGGTGGAGTCGGACGAGAAGCTGCAGGAGATTATCGGA
TTCGGGGGGGCTTTCACCGACGCCGCCACGATCAACTTGTTCAAGCTGCC
GGAGGATGTTCAGGAGAAGAGCAGCAGCAGCAGCAGCTCCGACGACGATG
ATGGCAGTGGCAGCAGCGACAGCGATGAGGAGGAGGAGGCTACAGCGGTC
TGCGCAGAGAGGGGTGTGTCGGGGACGGTGGTGGTGGAGTCGGACGAGAA
GCTGCAGGAGATTATCGGATTCGGGGGGGCTTTCACCGACGCCGCCACGA
TCAACTTGTTCAAGCTGCCGGAGGATGTTCAGGAGAAGGTGATGGACGCG
TACTTCGGACCCAACGGCATCGAGTACAGCGTCGGCCGCATCCCCATGGG
CAGCTGCGACTTCAGCGTGGAGCAGTACAGCTTCGACGAAGTACCGGGAG
ACTACAATCTCACGCACTTCGACGATGGCGTGGAGAAGGACACCGCTCAG
AGGGTAGGGGGGTGGGGAGAATAGGTGATGGACGCGTACTTCGGACCCAA
CGGCATCGAGTACAGCGTCGGCCGCATCCCCATGGGCAGCTGCGACTTCA
GCGTGGAGCAGTACAGCTTCGACGAAGTACCGGGAGACTACAATCTCACG
CACTTCGACGATGGCGTGGAGAAGGACACCGCTCAGAGGGTAGGGGGGTG
GGGAGAATAG
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