mRNA_E-fasciculatus_F_contig1431.3146.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig1431.3146.1
Unique NamemRNA_E-fasciculatus_F_contig1431.3146.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig1431.3146.1 vs. uniprot
Match: D7G710_ECTSI (General transcription and DNA repair factor IIH subunit TFB5 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G710_ECTSI)

HSP 1 Score: 139 bits (351), Expect = 9.470e-38
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 1 
Query:  289 MSTGYRSSKGVLITCDVPTKQFIMSLDARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVRPRT 498
            MSTGYRSSKGVLITCDVPTKQFIMSLDARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVRPRT
Sbjct:    1 MSTGYRSSKGVLITCDVPTKQFIMSLDARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVRPRT 70
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BLAST of mRNA_E-fasciculatus_F_contig1431.3146.1 vs. uniprot
Match: A0A835ZC95_9STRA (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZC95_9STRA)

HSP 1 Score: 75.5 bits (184), Expect = 1.170e-13
Identity = 39/69 (56.52%), Postives = 52/69 (75.36%), Query Frame = 1 
Query:  289 MSTGYRSSKGVLITCDVPTKQFIMSLDARLA--CVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVR 489
            MS    S KGVLITCDVPTKQFI+ L+++ A   +++DLD TH+FV+  +V EI+ +LE+L E NVYVR
Sbjct:    1 MSNVRTSRKGVLITCDVPTKQFILHLNSKQAQGFIINDLDETHLFVEASAVHEIREQLERLQEMNVYVR 69
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BLAST of mRNA_E-fasciculatus_F_contig1431.3146.1 vs. uniprot
Match: A0A383WPN6_TETOB (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Tetradesmus obliquus TaxID=3088 RepID=A0A383WPN6_TETOB)

HSP 1 Score: 57.4 bits (137), Expect = 3.600e-7
Identity = 26/67 (38.81%), Postives = 45/67 (67.16%), Query Frame = 1 
Query:  307 SSKGVLITCDVPTKQFIMSLD-----ARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVRP 492
            ++ G L+TCDVPTK+F++ L+      + A ++ +LD TH+ VK+D V+ +Q +++  + KNVY  P
Sbjct:    4 ATSGALLTCDVPTKEFLVWLNNQNKATKRAFIIKELDDTHLLVKEDMVEFVQEQVKAFNNKNVYTPP 70
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BLAST of mRNA_E-fasciculatus_F_contig1431.3146.1 vs. uniprot
Match: A0A6T6HXU1_9STRA (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A6T6HXU1_9STRA)

HSP 1 Score: 58.2 bits (139), Expect = 4.840e-7
Identity = 30/63 (47.62%), Postives = 42/63 (66.67%), Query Frame = 1 
Query:  307 SSKGVLITCDVPTKQFIMSLDARLAC----VVSDLDATHVFVKKDSVKEIQRELEQLHEKNVY 483
            +S G LITCDVPTKQ+I  L+   A     ++ DLDATH+ VKK +  EI+R +E   ++NV+
Sbjct:   36 ASAGYLITCDVPTKQYIQYLNEMKAVDKKFIIEDLDATHLLVKKKARAEIERRVESWMDENVF 98
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BLAST of mRNA_E-fasciculatus_F_contig1431.3146.1 vs. uniprot
Match: A0A2V3IN38_9FLOR (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3IN38_9FLOR)

HSP 1 Score: 58.2 bits (139), Expect = 5.200e-7
Identity = 30/73 (41.10%), Postives = 50/73 (68.49%), Query Frame = 1 
Query:  271 TRLRCAMSTGYRSSKGVLITCDVPTKQFIMSLDARLA--CVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVY 483
            TR +  M T   ++ GVLI+CD+P KQF++ +D +     ++SDLD THV VK+ ++  I+ +L++L+E+N Y
Sbjct:   41 TRTQSEMVT---ATAGVLISCDIPVKQFLLHVDEKDTGKFIISDLDETHVLVKESALDYIKSQLDKLYEENQY 110
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BLAST of mRNA_E-fasciculatus_F_contig1431.3146.1 vs. uniprot
Match: M2VWH3_GALSU (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Galdieria sulphuraria TaxID=130081 RepID=M2VWH3_GALSU)

HSP 1 Score: 56.6 bits (135), Expect = 5.940e-7
Identity = 27/60 (45.00%), Postives = 44/60 (73.33%), Query Frame = 1 
Query:  313 KGVLITCDVPTKQFIMSLDARLA---CVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVY 483
            +GVLITCD+P KQFI+ LD   A    V+++LD T +F++  ++K +Q +LE+L+E+N +
Sbjct:    6 RGVLITCDIPMKQFILKLDEDQAPNRFVIANLDETSLFIQSWALKLVQEKLEELYEENYF 65
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BLAST of mRNA_E-fasciculatus_F_contig1431.3146.1 vs. uniprot
Match: I0Z2U2_COCSC (General transcription and DNA repair factor IIH subunit TFB5 n=2 Tax=Coccomyxa TaxID=41891 RepID=I0Z2U2_COCSC)

HSP 1 Score: 53.1 bits (126), Expect = 1.050e-5
Identity = 27/65 (41.54%), Postives = 43/65 (66.15%), Query Frame = 1 
Query:  313 KGVLITCDVPTKQFIMSLD----ARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVRPR 495
            +GVL+ CDVPTK+FI+SL+         V+ DLD T +FV+   V+ +++ L++ +E+N Y  PR
Sbjct:    2 RGVLLQCDVPTKEFILSLNDSKPTNERFVILDLDDTRLFVQPTVVEWLEKRLKEFNEENTYQPPR 66
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BLAST of mRNA_E-fasciculatus_F_contig1431.3146.1 vs. uniprot
Match: A0A1X6PEZ7_PORUM (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Porphyra umbilicalis TaxID=2786 RepID=A0A1X6PEZ7_PORUM)

HSP 1 Score: 52.0 bits (123), Expect = 2.550e-5
Identity = 27/62 (43.55%), Postives = 41/62 (66.13%), Query Frame = 1 
Query:  307 SSKGVLITCDVPTKQFIMSLDARLA---CVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVY 483
            ++ GVL+TCDVP KQF++ LD   A    +V+ LD TH+F+   +V  I+ +LE+L  +N Y
Sbjct:    4 ATHGVLVTCDVPVKQFLLKLDNDRAPDRFIVAKLDETHLFIHAWAVDMIKDQLERLFAENQY 65
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BLAST of mRNA_E-fasciculatus_F_contig1431.3146.1 vs. uniprot
Match: A4S7H4_OSTLU (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Ostreococcus lucimarinus (strain CCE9901) TaxID=436017 RepID=A4S7H4_OSTLU)

HSP 1 Score: 50.8 bits (120), Expect = 7.600e-5
Identity = 29/63 (46.03%), Postives = 37/63 (58.73%), Query Frame = 1 
Query:  316 GVLITCDVPTKQFIMSLD----ARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVRP 492
            GV+IT DVP KQ+I+ L+    A    VV DLDA  V V+ D+V  I RE+E   +   YV P
Sbjct:    7 GVMITADVPIKQYILHLNDVAPAERKFVVVDLDARRVLVQPDAVDAIHREIEAFQQSTQYVAP 69
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BLAST of mRNA_E-fasciculatus_F_contig1431.3146.1 vs. uniprot
Match: B5Y3U5_PHATC (General transcription and DNA repair factor IIH subunit TFB5 n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B5Y3U5_PHATC)

HSP 1 Score: 51.6 bits (122), Expect = 9.660e-5
Identity = 29/79 (36.71%), Postives = 45/79 (56.96%), Query Frame = 1 
Query:  259 RAPSTRLRCAMSTGYRSSKGVLITCDVPTKQFIMSLD----ARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVY 483
            R+P  R +         S G L++CDVPTKQFI  L+         ++ DLDATH+ V+  +  EI+R++E+  + NV+
Sbjct:   20 RSPRHRPKIHNDDDLPPSAGYLVSCDVPTKQFIQHLNDLKPVDKKFILQDLDATHLLVELKARDEIRRKVEEWEDSNVF 98
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig1431.3146.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 10
Match NameE-valueIdentityDescription
D7G710_ECTSI9.470e-38100.00General transcription and DNA repair factor IIH su... [more]
A0A835ZC95_9STRA1.170e-1356.52General transcription and DNA repair factor IIH su... [more]
A0A383WPN6_TETOB3.600e-738.81General transcription and DNA repair factor IIH su... [more]
A0A6T6HXU1_9STRA4.840e-747.62General transcription and DNA repair factor IIH su... [more]
A0A2V3IN38_9FLOR5.200e-741.10General transcription and DNA repair factor IIH su... [more]
M2VWH3_GALSU5.940e-745.00General transcription and DNA repair factor IIH su... [more]
I0Z2U2_COCSC1.050e-541.54General transcription and DNA repair factor IIH su... [more]
A0A1X6PEZ7_PORUM2.550e-543.55General transcription and DNA repair factor IIH su... [more]
A4S7H4_OSTLU7.600e-546.03General transcription and DNA repair factor IIH su... [more]
B5Y3U5_PHATC9.660e-536.71General transcription and DNA repair factor IIH su... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig1431contigE-fasciculatus_F_contig1431:9826..12604 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionTFIIH subunit TTDA/Tfb5
Ec32 orthologEc-01_011510.1
Exons3
Model size1137
Cds size213
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig1431.3146.1prot_E-fasciculatus_F_contig1431.3146.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig1431 10114..11968 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927922.251541-UTR-E-fasciculatus_F_contig1431:9825..101131622927922.251541-UTR-E-fasciculatus_F_contig1431:9825..10113Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig1431 9826..10113 +
1695995752.079321-UTR-E-fasciculatus_F_contig1431:9825..101131695995752.079321-UTR-E-fasciculatus_F_contig1431:9825..10113Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig1431 9826..10113 +
1622927922.349308-UTR-E-fasciculatus_F_contig1431:11968..126041622927922.349308-UTR-E-fasciculatus_F_contig1431:11968..12604Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig1431 11969..12604 +
1695995752.1178153-UTR-E-fasciculatus_F_contig1431:11968..126041695995752.1178153-UTR-E-fasciculatus_F_contig1431:11968..12604Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig1431 11969..12604 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927922.3029132-CDS-E-fasciculatus_F_contig1431:10113..101571622927922.3029132-CDS-E-fasciculatus_F_contig1431:10113..10157Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1431 10114..10157 +
1695995752.0883904-CDS-E-fasciculatus_F_contig1431:10113..101571695995752.0883904-CDS-E-fasciculatus_F_contig1431:10113..10157Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1431 10114..10157 +
1622927922.319869-CDS-E-fasciculatus_F_contig1431:11435..115491622927922.319869-CDS-E-fasciculatus_F_contig1431:11435..11549Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1431 11436..11549 +
1695995752.0988293-CDS-E-fasciculatus_F_contig1431:11435..115491695995752.0988293-CDS-E-fasciculatus_F_contig1431:11435..11549Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1431 11436..11549 +
1622927922.3346488-CDS-E-fasciculatus_F_contig1431:11913..119681622927922.3346488-CDS-E-fasciculatus_F_contig1431:11913..11968Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1431 11914..11968 +
1695995752.1074533-CDS-E-fasciculatus_F_contig1431:11913..119681695995752.1074533-CDS-E-fasciculatus_F_contig1431:11913..11968Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig1431 11914..11968 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig1431.3146.1

>prot_E-fasciculatus_F_contig1431.3146.1 ID=prot_E-fasciculatus_F_contig1431.3146.1|Name=mRNA_E-fasciculatus_F_contig1431.3146.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=71bp
MSTGYRSSKGVLITCDVPTKQFIMSLDARLACVVSDLDATHVFVKKDSVK
EIQRELEQLHEKNVYVRPRT*
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mRNA from alignment at E-fasciculatus_F_contig1431:9826..12604+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig1431.3146.1 ID=mRNA_E-fasciculatus_F_contig1431.3146.1|Name=mRNA_E-fasciculatus_F_contig1431.3146.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=2779bp|location=Sequence derived from alignment at E-fasciculatus_F_contig1431:9826..12604+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
CGTGGACAGCACAACTCCTGGTTAACTGCTGTGGGTGACGTCTTCACCAA
AGGACGCCATGTGTTTCTGAAACGAGCGTACAGAGAGCCGGAGAGCCGAG
CGTATGCACACAAAATTGGTACAGCAGCAGTGTAACGGTGCGGGATTTTC
AGAACAGGAGACAGGAACGTTCTATCTCCTACTCGCCGACCGACGGCAAG
CACAAAAAATCCGACAGTCCCGGAAGCAACCAACAACATCCGAGCACTGA
CTGAGTACCGAGCACCGAGCACACGGCTGCGTTGTGCGATGAGCACAGGA
TACCGGAGCAGCAAAGGCGTTCTCATCACCTGGTACGGTACGGAAAGGGA
ACTGGTCTGTTGCATGCCCTGAGTCGAAGCCTGCCCCAATCATAGCAGCT
TGTCAAGTGATCGCATAGTGGGTCGACTGATGTACATGTACCGTCCGTAG
ACTGCTGTAGACACAGTGTCTAGACGGTGTAGTGCATTAGAGTACTGCTA
CATCAACAGCCTTGTTCTTTCACTGGTAGTTCTATTGCTACTGTACCGAA
CTGCCAATGTGGCTCAACATTGCGGGCTACGATACTGACGCTGGTCATGC
CGTTTGTTGCTTAAGATACCACCCCCCTTGGGACCGGTTTACTCTATGTA
TGCATACCTACCGGAATACACGTAGCGCAGCCACGTTCAGCAGCGACGTC
GCCGCGGCGGCTTGGGCACCGTCTCGTCGAAAGCTTTCCAAAAACGTGCC
CTTCGACGGTGGCATCCTCTTCCGTTGTGGAGTAATTGAGCTTTGGAAAA
TCTGTCACGGGGGTGTTGCATCTTGTGCAACCTACATCAGGATGAACAAA
GTTCATTTCTGTATTTGAAACCATGGGCGCCCGTGTTACGGCCTATTGCA
TTATTAGGAAAAATTGCATGTAGGGAATGCAGAGAGCGGTGCTGTTGTTT
AACGCGTGTATTTACGCATGCGCGTGAAGTCCACAGGTTGGAAATTTGCT
GAACTCCTCTTAGAGATGCACATGCGAAGCAGCGGAGCATGTAGTAATTG
GCGGTCTTCTCTTGCAGGCACAGTAGCAGTGGTGATGGCGTTCCAGAAAT
GTTTTTTTTTTTCACGGTGACAAACAATTCGCTTTGGCACCTCAAGGGGG
AACCCGCCGTCTACAAAAACATGGCCCAGAACATTGTCGTGGGGTTGATC
TATTTCGCCGCTCTCGTTGTCTTCCCTCCATCGTCGTCGTTCTCTTGTGC
TTGGCATTGCCCGATATAACGTGGTGTTTCACTGTATGTAGCTGCGCATG
TACCATGTAGCTGCGCATGTACCCTGTTTACGTTTTCCCTCTCAGGCAAA
ATCAAGGGCAGGGTAGAATCCATTCTATGTCTTTTTTGAAGAGTCCGAAC
TCGTTTTTGTTCAAGTTTTCGGCGCTCGATATCGCTATGATGACTGGTAT
TATTCCCCCGCCTCCGCCCCTTCCTACCCAGCGCTGTCCCTCGTTTTGAT
GTTCGTGTTGGGTGTTCTGACGCGAAAACGATGGCTGTGTGGACTTTCGC
CGTTTTCGTTGGAGTTGATAACCAACCATGTTGCTTTATTTGTCCCCTTG
TTGTATCCAGCGATGTGCCCACGAAGCAGTTCATTATGAGCCTGGACGCA
CGGCTGGCGTGCGTGGTGTCGGACCTGGACGCCACACATGTCTTCGTCAA
GAAAGACAGCGTCAAAGAGATACAGTGAGTGCTATCCTGCCCACCTGGTT
GTCTGTACAAGTTTGGCAGGAATCAACTTGTAGGAGAAGGCGCATGCGTG
TGTCGAGCTCTGAAATGAGTTGTTTCAGTTCTATGTTTGCTTTTTGTATT
GTTTTAGAGAAAGCGATGCCGATGTTACGGACTGCGCCGTGGTTGCACTG
TATGTAGTGGGTGTCCTTATCCATATTGTCCAGAAGTTACGCTACATACA
ATTTATTGCGGAGTGAGTTGTGGACGTGTGCAAGGGGTAAAAGGGACATA
TTATATTTCGGCCGCGGTGATGCTCGCTTTGGGGTATACCTAGCCATCAA
CGACCATTCGCCGCATTCCATGCCTCATCCGTTTTCAGGCGCGAGCTGGA
ACAGCTGCACGAGAAAAACGTTTACGTGCGGCCTCGAACATAGATAGCAA
CAGCGGCGGTACGCAGTAGTGCGCAACAGGAAGCAGAAAGACGGCACCGG
CACGACGTCTGCAGGGTCGTTGTACTCACCACGCGGACGTTTCATTTCGC
GTGGCAGAGAGCAGGAAAACTGGCGAAGAGAGGGCGGCTTTCAATTTTTT
TTTGTTGGAGCAAGCAGTGTTGAAGGGACGTTGCAGGAGGAAGTCGTGCA
GCTTACTGAGAGGGGGGTTCGCGATCTCTACTGCTGTGCTCGCACTGCTG
TCTGTGTGATCGGCGGTAGTGTGTCTCTGTTCGGATTTCACACCTGGACG
AGCTATGTCTTGCTTCGACGAGTTTGCAAGCCCCGTACCGTTGATAAGGT
GATGGTACCTCGTTGAAGAAGGGCATTACAGCAGTGTAGTAAAGCGTACA
ATCAATAGGACCGTGCCCAGTTGCACGCAGCATCTCGCAACCATCGTGGC
CATGTGGTGTGGAGATACGTATTGTGGAGGATTGAGACACCCTTGTCACT
GTAGCATCATCAATGAAGCGTGTTCTGTGTAGCAAGCCTCCGTCCTAGCT
GGTTGAAGTTTCTGTCGTCACCAGATAGACTACAGCCCACATACATTGGC
GTGGAAGGAGGCCTTTGGAGCCATGGATG
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig1431:9826..12604+

>mRNA_E-fasciculatus_F_contig1431.3146.1 ID=mRNA_E-fasciculatus_F_contig1431.3146.1|Name=mRNA_E-fasciculatus_F_contig1431.3146.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=426bp|location=Sequence derived from alignment at E-fasciculatus_F_contig1431:9826..12604+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
ATGAGCACAGGATACCGGAGCAGCAAAGGCGTTCTCATCACCTGATGAGC
ACAGGATACCGGAGCAGCAAAGGCGTTCTCATCACCTGCGATGTGCCCAC
GAAGCAGTTCATTATGAGCCTGGACGCACGGCTGGCGTGCGTGGTGTCGG
ACCTGGACGCCACACATGTCTTCGTCAAGAAAGACAGCGTCAAAGAGATA
CACGATGTGCCCACGAAGCAGTTCATTATGAGCCTGGACGCACGGCTGGC
GTGCGTGGTGTCGGACCTGGACGCCACACATGTCTTCGTCAAGAAAGACA
GCGTCAAAGAGATACAGCGCGAGCTGGAACAGCTGCACGAGAAAAACGTT
TACGTGCGGCCTCGAACATAGGCGCGAGCTGGAACAGCTGCACGAGAAAA
ACGTTTACGTGCGGCCTCGAACATAG
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