mRNA_E-fasciculatus_F_contig13834.2825.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig13834.2825.1
Unique NamemRNA_E-fasciculatus_F_contig13834.2825.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig13834.2825.1 vs. uniprot
Match: A0A6H5KNI6_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KNI6_9PHAE)

HSP 1 Score: 141 bits (356), Expect = 2.510e-32
Identity = 79/149 (53.02%), Postives = 95/149 (63.76%), Query Frame = 3 
Query:    9 RWYTSTLELQLVSLAFCGECRSVPTLHVHIGEATPSSLWSHHESHKTPDECSREDNISSRVPAVEAFSWTWAFEIPEEALLQRST---------------AFAACLASALWPQTLKRLVFDSNMPVDTALWPASLQQISFGYGFDKPIV 410
            RWY STLELQLVSL  CG CR V TLHV +   TP SLW   ++H T +  S +  ISSRVP V+AF  TWA  +P   L QRS                + AA L SA+WPQ LKR+VF+S+M V+TA WPASLQQ+SFG GF++ IV
Sbjct:   27 RWYVSTLELQLVSLEVCGACRRVQTLHVRVKPQTPPSLWFPSQNHTTTNNRSPKVCISSRVPVVQAFGLTWA--LPMGVLQQRSATEVRSGSGCRRANTHSSAASLESAVWPQRLKRMVFESDMTVNTASWPASLQQLSFGCGFNQAIV 173
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BLAST of mRNA_E-fasciculatus_F_contig13834.2825.1 vs. uniprot
Match: A0A6H5KLV1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KLV1_9PHAE)

HSP 1 Score: 135 bits (340), Expect = 1.260e-31
Identity = 78/147 (53.06%), Postives = 90/147 (61.22%), Query Frame = 3 
Query:    9 RWYTSTLELQLVSLAFCGECRSVPTLHVHIGEATPSSLWSHHESHKTPDECSREDNISSRVPAVEAFSWTWAFEIPEEALLQRST---------------AFAACLASALWPQT-LKRLVFDSNMPVDTALWPASLQQISFGYGFDK 401
            RWY STLELQLVSL FCG CR VPTLHV +   TP SLWS  +SH T  + SRE  +S+RVPAV+A   TWA ++  E LLQ S                +  A L S  W Q  LKRLVFDS+MP  TA WPASL Q+ FG  FD+
Sbjct:   35 RWYASTLELQLVSLEFCGACRRVPTLHVRVDRQTPRSLWSPCKSHATTKKRSREACVSNRVPAVQASGLTWALDM--EVLLQTSAVELRSRSKYRRVSGNSSTASLGSMFWLQMMLKRLVFDSDMPASTASWPASLPQLLFGDRFDQ 179
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BLAST of mRNA_E-fasciculatus_F_contig13834.2825.1 vs. uniprot
Match: A0A6H5K8K9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K8K9_9PHAE)

HSP 1 Score: 117 bits (293), Expect = 1.860e-24
Identity = 70/149 (46.98%), Postives = 84/149 (56.38%), Query Frame = 3 
Query:    9 RWYTSTLELQLVSLAFCGECRSVPTLHVHIGEATPSSLWSHHESHKTPDECSREDNISSRVPAVEAFSWTWAFEIPEEALLQRST---------------AFAACLASALWPQTLKRLVFDSNMPVDTALWPASLQQISFGYGFDKPIV 410
            RWY STLELQLVSL FCG CRS  +  V I   TP SLWS  +SH T  + SRE  I SRVPAV+A  W WA  +P E LL RS                + A       W Q L RL+F S   V++  WPA LQ++SFG  F++PI+
Sbjct:   47 RWYVSTLELQLVSLEFCGACRSALSFDVQIHRQTPHSLWSPRKSHGTLKKRSREACIPSRVPAVQANGWMWA--LPMEILLPRSATELHQVSENRQPTGPSSATSPGRMGWFQKLTRLMFYSERSVNSVSWPACLQELSFGDFFNQPII 193
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BLAST of mRNA_E-fasciculatus_F_contig13834.2825.1 vs. uniprot
Match: A0A6H5L3M5_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L3M5_9PHAE)

HSP 1 Score: 112 bits (281), Expect = 5.170e-23
Identity = 67/150 (44.67%), Postives = 85/150 (56.67%), Query Frame = 3 
Query:    9 RWYTSTLELQLVSLAFCGECRSVPTLHVHIGEATPSSLWSHHES--HKTPDECSREDNISSRVPAVEAFSWTWAFEIPEEALLQ--------------RSTAFAACLASALWPQTLKRLVFDSNMPVDTALWPASLQQISFGYGFDKPIV 410
            RWYTS  ELQLVSL FCG CRS   L V +    P SLWS  +S  +  PD         +RVPAV A+ WTW+  IP + LL+              R +   A LAS +WPQ LK+L FD +  V+ A WPASLQ ++FG  F++PI+
Sbjct:  122 RWYTSIRELQLVSLKFCGVCRSTSILRVRVEHNAPPSLWSPTKSFSYALPD-------AWNRVPAVRAYGWTWS--IPVDTLLKVCDLRYWAKAELSRRGSFPEAKLASVVWPQGLKQLEFDLDTAVEKASWPASLQSLTFGCSFNQPIL 262
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BLAST of mRNA_E-fasciculatus_F_contig13834.2825.1 vs. uniprot
Match: A0A6H5L1V9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L1V9_9PHAE)

HSP 1 Score: 102 bits (253), Expect = 1.100e-19
Identity = 61/147 (41.50%), Postives = 83/147 (56.46%), Query Frame = 3 
Query:    9 RWYTSTLELQLVSLAFCGECRSVPTLHVHIGEATPSSLWSHHESHKTPDECSREDNISSRVPAVEAFSWTWA--FEIPE-----------EALLQRSTAFAACLASALWPQTLKRLVFDSNM---PVDTALWPASLQQISFGYGFDK 401
            RWYTSTL+LQL SL FCG CRS P LHV++   TP +LWS  +++ T          S+RVPAV+A   TWA   E P            E+L  + +   A L S +WPQ L+RLV D  +   P++   WP+SL+ ++F   FD+
Sbjct:   27 RWYTSTLQLQLASLEFCGACRSTPMLHVYVDAETPPTLWSLGQANPTA-------GTSTRVPAVQAVGLTWARPTESPLGSASSELWTRLESLQLQVSLCTANLDSVVWPQGLQRLVMDCTVFIIPLEMMSWPSSLKSLTFAGIFDQ 166
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BLAST of mRNA_E-fasciculatus_F_contig13834.2825.1 vs. uniprot
Match: A0A6H5K2L2_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K2L2_9PHAE)

HSP 1 Score: 95.1 bits (235), Expect = 4.860e-18
Identity = 65/144 (45.14%), Postives = 74/144 (51.39%), Query Frame = 3 
Query:    9 RWYTSTLELQLVSLAFCGECRSVPTLHVHIGEATPSSLWSHHESHKTPDECSREDNISSRVPAVEAFSWTWAFEIPE-----EALLQRSTAF--------AACLASALWPQTLKRLVFDSNMPVDTALWPASLQQISFGYGFDK 401
            RWY STLELQ VSL FC  CR VPT H     +T   L S                  SR P V A+S TWA  +          LQR + +        AA LAS LW Q LKRLV + + PV TA WPASLQQ+SFGY F+K
Sbjct:   37 RWYASTLELQPVSLEFCVACRRVPTSH---STSTVGRLVSG----------------VSRAPVVRAYSLTWALSMDVLLQSCAMELQRGSKYRRSGGHSSAANLASMLWLQRLKRLVLELDTPVITASWPASLQQLSFGYKFNK 161
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BLAST of mRNA_E-fasciculatus_F_contig13834.2825.1 vs. uniprot
Match: A0A6H5JL88_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JL88_9PHAE)

HSP 1 Score: 82.8 bits (203), Expect = 1.990e-13
Identity = 59/160 (36.88%), Postives = 82/160 (51.25%), Query Frame = 3 
Query:    3 HNRWYTSTLELQLVSLAFCGECRSVPTLHVHIGEATPSSLWSHHESHKTPDE-------CSREDNISSRVPAVEAFSWTWAFEIPEEALLQRSTAFA----------------ACLASALWPQTLKRLVF--DSNMPVDTALWPASLQQISFGYGFDKPI 407
            +NRW+TSTL +QL SL FC   RS+PT+ +H+    P SLW   +S +TP         C +   +S RVP + A    W   +  E LLQ STA                  A  AS + PQ L++L    D+   V+   WPA LQ++SFG+ F++PI
Sbjct:    2 NNRWFTSTLNIQLASLEFCRASRSIPTIALHVDSRIPRSLWDGRKS-QTPSSTRGLEIRCWKARPLS-RVPLIRASRLNWGLSM--ETLLQ-STAVELWTGLKSLHLCGDVSDASPASMVQPQRLEQLEGNKDARDSVEAVPWPAFLQKLSFGFPFNRPI 156
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BLAST of mRNA_E-fasciculatus_F_contig13834.2825.1 vs. uniprot
Match: D7G0Z9_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G0Z9_ECTSI)

HSP 1 Score: 68.9 bits (167), Expect = 1.670e-10
Identity = 30/40 (75.00%), Postives = 31/40 (77.50%), Query Frame = 3 
Query:    9 RWYTSTLELQLVSLAFCGECRSVPTLHVHIGEATPSSLWS 128
            RWY STLELQLVSL FCG CR VPTLHV +   TP SLWS
Sbjct:   59 RWYASTLELQLVSLEFCGACRHVPTLHVRVDRRTPRSLWS 98
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BLAST of mRNA_E-fasciculatus_F_contig13834.2825.1 vs. uniprot
Match: A0A6H5KLS8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KLS8_9PHAE)

HSP 1 Score: 69.3 bits (168), Expect = 2.040e-9
Identity = 41/89 (46.07%), Postives = 53/89 (59.55%), Query Frame = 3 
Query:  180 SSRVPAVEAFSWTWAFEIPEEALLQRST---------------AFAACLASALWPQTLKRLVFDSNMPVDTALWPASLQQISFGYGFDK 401
            SSRVPAV+AF   W  E+P E LLQR+                + A+ L  A+ P  LKRL FDS+MPV+T  WPASLQ++SF   F++
Sbjct:    9 SSRVPAVQAFGLKW--ELPVEVLLQRAATKPRTGVECQRPTGPSSASRLQRAIRPPNLKRLEFDSDMPVNTVSWPASLQELSFDTEFNQ 95
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BLAST of mRNA_E-fasciculatus_F_contig13834.2825.1 vs. uniprot
Match: A0A6H5JSE5_9PHAE (Reverse transcriptase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JSE5_9PHAE)

HSP 1 Score: 70.9 bits (172), Expect = 3.600e-9
Identity = 35/76 (46.05%), Postives = 44/76 (57.89%), Query Frame = 3 
Query:    9 RWYTSTLELQLVSLAFCGECRSVPTLHVHIGEATPSSLWSHHESHKTPDECSREDNI------SSRVPAVEAFSWT 218
            +WY STLELQLVSLAFCG CRS   LH+H+    P SLW  H+S+ TP++      +      +  V A E   WT
Sbjct:  993 QWYASTLELQLVSLAFCGACRSAKMLHLHVDRGKPPSLWFPHKSYTTPNKRIAASRVWFDVGAADGVSAAEFCHWT 1068
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig13834.2825.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 11
Match NameE-valueIdentityDescription
A0A6H5KNI6_9PHAE2.510e-3253.02Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5KLV1_9PHAE1.260e-3153.06Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5K8K9_9PHAE1.860e-2446.98Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5L3M5_9PHAE5.170e-2344.67Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5L1V9_9PHAE1.100e-1941.50Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5K2L2_9PHAE4.860e-1845.14Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JL88_9PHAE1.990e-1336.88Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G0Z9_ECTSI1.670e-1075.00Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5KLS8_9PHAE2.040e-946.07Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JSE5_9PHAE3.600e-946.05Reverse transcriptase domain-containing protein n=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig13834contigE-fasciculatus_F_contig13834:2..1503 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Exons1
Model size1502
Cds size867
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig13834.2825.1prot_E-fasciculatus_F_contig13834.2825.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig13834 334..1200 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927877.4235928-UTR-E-fasciculatus_F_contig13834:1..3331622927877.4235928-UTR-E-fasciculatus_F_contig13834:1..333Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig13834 2..333 +
1695995734.1210523-UTR-E-fasciculatus_F_contig13834:1..3331695995734.1210523-UTR-E-fasciculatus_F_contig13834:1..333Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig13834 2..333 +
1622927877.4495811-UTR-E-fasciculatus_F_contig13834:1200..15031622927877.4495811-UTR-E-fasciculatus_F_contig13834:1200..1503Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig13834 1201..1503 +
1695995734.1386542-UTR-E-fasciculatus_F_contig13834:1200..15031695995734.1386542-UTR-E-fasciculatus_F_contig13834:1200..1503Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleUTRE-fasciculatus_F_contig13834 1201..1503 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927877.434672-CDS-E-fasciculatus_F_contig13834:333..12001622927877.434672-CDS-E-fasciculatus_F_contig13834:333..1200Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig13834 334..1200 +
1695995734.130746-CDS-E-fasciculatus_F_contig13834:333..12001695995734.130746-CDS-E-fasciculatus_F_contig13834:333..1200Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig13834 334..1200 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig13834.2825.1

>prot_E-fasciculatus_F_contig13834.2825.1 ID=prot_E-fasciculatus_F_contig13834.2825.1|Name=mRNA_E-fasciculatus_F_contig13834.2825.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=289bp
MPVDTALWPASLQQISFGYGFDKPIVDVVWPASLQQLSLGDSFDQPIVGA
VLPGSLQRLSFGVCFNQPIVGAVLPASLQQLSFGQSFNQPIAGAVLPASL
QQLSFGKFFNQPIVYVVWPASLQQLSFGQRFNQPIARVVWPDSLQRLSFG
PFLNEPIVGVVWPASLQQLSLGNSFNQPIVGVVWPDSLQQLSFGNSFNRP
IIGVVWPDSLQKLSFGSDFNQPIVGVVWPDSLQTLLFQGRFNQPVVGVVW
PTSLQHLELRVAFKQSLAEVVWPTSLRSLICYGRRRML*
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mRNA from alignment at E-fasciculatus_F_contig13834:2..1503+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig13834.2825.1 ID=mRNA_E-fasciculatus_F_contig13834.2825.1|Name=mRNA_E-fasciculatus_F_contig13834.2825.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=1502bp|location=Sequence derived from alignment at E-fasciculatus_F_contig13834:2..1503+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
AACACAACCGTTGGTATACGTCTACACTGGAACTACAGCTTGTGTCGCTG
GCGTTTTGTGGGGAGTGCAGAAGCGTCCCGACCTTGCACGTGCACATCGG
TGAAGCAACCCCTTCTAGTTTGTGGTCCCATCACGAATCGCACAAAACAC
CAGACGAGTGCTCTCGAGAGGATAACATCTCGAGTCGCGTGCCGGCGGTC
GAGGCTTTTTCTTGGACATGGGCATTTGAAATTCCGGAGGAGGCTCTGCT
GCAGCGTTCTACCGCTTTTGCAGCATGTCTAGCATCGGCGCTTTGGCCTC
AAACACTCAAGCGACTGGTGTTTGATTCCAATATGCCCGTGGATACGGCC
TTGTGGCCTGCCTCCCTGCAGCAGATATCGTTTGGGTATGGATTCGACAA
GCCCATTGTCGATGTCGTGTGGCCTGCCTCTCTGCAGCAGCTATCGCTCG
GTGATAGCTTCGACCAGCCCATCGTCGGAGCCGTGTTGCCGGGCTCCTTG
CAACGGCTTTCGTTTGGAGTGTGCTTCAACCAGCCCATCGTCGGAGCCGT
GTTGCCGGCTTCCCTGCAGCAGCTATCATTTGGACAATCGTTCAATCAGC
CCATAGCCGGAGCCGTGTTGCCGGCCTCCCTGCAGCAGCTATCGTTTGGA
AAGTTCTTCAACCAACCCATCGTATATGTCGTGTGGCCGGCCTCCCTCCA
GCAACTATCGTTTGGACAACGCTTCAACCAGCCCATCGCCCGAGTCGTGT
GGCCGGACTCCCTGCAGCGGCTATCGTTTGGACCTTTCTTGAACGAGCCC
ATCGTCGGAGTCGTGTGGCCGGCCTCTCTGCAGCAGCTATCGCTCGGGAA
TAGCTTCAACCAGCCCATCGTCGGAGTCGTGTGGCCGGACTCCTTGCAGC
AGCTATCGTTTGGGAATAGCTTCAACCGGCCCATCATCGGAGTCGTGTGG
CCGGACTCCCTTCAAAAGCTATCCTTTGGTTCTGATTTCAACCAGCCCAT
CGTAGGAGTCGTGTGGCCGGACTCCTTGCAAACGTTGTTGTTTCAGGGGC
GCTTCAACCAGCCCGTGGTCGGAGTGGTGTGGCCGACCTCACTTCAGCAT
TTAGAACTCCGTGTTGCGTTCAAGCAATCCTTGGCCGAAGTGGTATGGCC
GACTTCCTTGCGAAGTTTGATTTGTTATGGACGACGCCGAATGCTGTGAC
AACTGTGAAAGGAGAAGATTTGTTGCAGGAGCAGACGGAAACAAATCAAG
GTTTATTGAAAGCAATCGAATTTGTAACGTCGTTGTTAGGGTATGTAGAA
ATGGCTACGGAAGCACGGGGCCCCCCTCCCAAGCGGGAGCATGGGAGTAG
CCACTACCTCGGCACTATGTCGAAGCTAGGTGTTCACACAACGTCTTACG
AGGATTGGTCTAGAGAGAGCAACGCAGAGACAAGGAGCGAGTGGACACTT
TGAGAGCTTCGAGTGGTTTGCCAGCTGGAGGAGCGGCAAACAAGTTTGGT
CT
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig13834:2..1503+

>mRNA_E-fasciculatus_F_contig13834.2825.1 ID=mRNA_E-fasciculatus_F_contig13834.2825.1|Name=mRNA_E-fasciculatus_F_contig13834.2825.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=1734bp|location=Sequence derived from alignment at E-fasciculatus_F_contig13834:2..1503+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
ATGCCCGTGGATACGGCCTTGTGGCCTGCCTCCCTGCAGCAGATATCGTT
TGGGTATGGATTCGACAAGCCCATTGTCGATGTCGTGTGGCCTGCCTCTC
TGCAGCAGCTATCGCTCGGTGATAGCTTCGACCAGCCCATCGTCGGAGCC
GTGTTGCCGGGCTCCTTGCAACGGCTTTCGTTTGGAGTGTGCTTCAACCA
GCCCATCGTCGGAGCCGTGTTGCCGGCTTCCCTGCAGCAGCTATCATTTG
GACAATCGTTCAATCAGCCCATAGCCGGAGCCGTGTTGCCGGCCTCCCTG
CAGCAGCTATCGTTTGGAAAGTTCTTCAACCAACCCATCGTATATGTCGT
GTGGCCGGCCTCCCTCCAGCAACTATCGTTTGGACAACGCTTCAACCAGC
CCATCGCCCGAGTCGTGTGGCCGGACTCCCTGCAGCGGCTATCGTTTGGA
CCTTTCTTGAACGAGCCCATCGTCGGAGTCGTGTGGCCGGCCTCTCTGCA
GCAGCTATCGCTCGGGAATAGCTTCAACCAGCCCATCGTCGGAGTCGTGT
GGCCGGACTCCTTGCAGCAGCTATCGTTTGGGAATAGCTTCAACCGGCCC
ATCATCGGAGTCGTGTGGCCGGACTCCCTTCAAAAGCTATCCTTTGGTTC
TGATTTCAACCAGCCCATCGTAGGAGTCGTGTGGCCGGACTCCTTGCAAA
CGTTGTTGTTTCAGGGGCGCTTCAACCAGCCCGTGGTCGGAGTGGTGTGG
CCGACCTCACTTCAGCATTTAGAACTCCGTGTTGCGTTCAAGCAATCCTT
GGCCGAAGTGGTATGGCCGACTTCCTTGCGAAGTTTGATTTGTTATGGAC
GACGCCGAATGCTGTGAATGCCCGTGGATACGGCCTTGTGGCCTGCCTCC
CTGCAGCAGATATCGTTTGGGTATGGATTCGACAAGCCCATTGTCGATGT
CGTGTGGCCTGCCTCTCTGCAGCAGCTATCGCTCGGTGATAGCTTCGACC
AGCCCATCGTCGGAGCCGTGTTGCCGGGCTCCTTGCAACGGCTTTCGTTT
GGAGTGTGCTTCAACCAGCCCATCGTCGGAGCCGTGTTGCCGGCTTCCCT
GCAGCAGCTATCATTTGGACAATCGTTCAATCAGCCCATAGCCGGAGCCG
TGTTGCCGGCCTCCCTGCAGCAGCTATCGTTTGGAAAGTTCTTCAACCAA
CCCATCGTATATGTCGTGTGGCCGGCCTCCCTCCAGCAACTATCGTTTGG
ACAACGCTTCAACCAGCCCATCGCCCGAGTCGTGTGGCCGGACTCCCTGC
AGCGGCTATCGTTTGGACCTTTCTTGAACGAGCCCATCGTCGGAGTCGTG
TGGCCGGCCTCTCTGCAGCAGCTATCGCTCGGGAATAGCTTCAACCAGCC
CATCGTCGGAGTCGTGTGGCCGGACTCCTTGCAGCAGCTATCGTTTGGGA
ATAGCTTCAACCGGCCCATCATCGGAGTCGTGTGGCCGGACTCCCTTCAA
AAGCTATCCTTTGGTTCTGATTTCAACCAGCCCATCGTAGGAGTCGTGTG
GCCGGACTCCTTGCAAACGTTGTTGTTTCAGGGGCGCTTCAACCAGCCCG
TGGTCGGAGTGGTGTGGCCGACCTCACTTCAGCATTTAGAACTCCGTGTT
GCGTTCAAGCAATCCTTGGCCGAAGTGGTATGGCCGACTTCCTTGCGAAG
TTTGATTTGTTATGGACGACGCCGAATGCTGTGA
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