mRNA_E-fasciculatus_F_contig13357.2518.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig13357.2518.1
Unique NamemRNA_E-fasciculatus_F_contig13357.2518.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig13357.2518.1 vs. uniprot
Match: D8LDD4_ECTSI (PDZ domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LDD4_ECTSI)

HSP 1 Score: 124 bits (310), Expect = 1.210e-33
Identity = 57/59 (96.61%), Postives = 57/59 (96.61%), Query Frame = 1 
Query:    1 AKTGDNLRKTLLAGKVEVYDMVGKMTNCNGGGQCGTCAVDVVEAEGWDPRSEWEAGKLK 177
            AKTGDNLRKTLLAGKVEVYDMVGKMTNCNGGGQCGTC V VVEAEGWDPRSEWEAGKLK
Sbjct:  175 AKTGDNLRKTLLAGKVEVYDMVGKMTNCNGGGQCGTCVVQVVEAEGWDPRSEWEAGKLK 233
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BLAST of mRNA_E-fasciculatus_F_contig13357.2518.1 vs. uniprot
Match: A0A836CP36_9STRA (2Fe-2S ferredoxin-type domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CP36_9STRA)

HSP 1 Score: 82.4 bits (202), Expect = 9.780e-18
Identity = 37/59 (62.71%), Postives = 45/59 (76.27%), Query Frame = 1 
Query:    1 AKTGDNLRKTLLAGKVEVYDMVGKMTNCNGGGQCGTCAVDVVEAEGWDPRSEWEAGKLK 177
            A+ GDNLRK L+AGKV +Y+ +  MTNCNG GQCGTC   VV  +GW PRS++EA KLK
Sbjct:  154 ARNGDNLRKVLMAGKVSIYEGLAVMTNCNGAGQCGTCVTKVV-GQGWSPRSDYEANKLK 211
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BLAST of mRNA_E-fasciculatus_F_contig13357.2518.1 vs. uniprot
Match: A0A7S3YA98_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3YA98_HETAK)

HSP 1 Score: 80.9 bits (198), Expect = 5.400e-17
Identity = 40/59 (67.80%), Postives = 45/59 (76.27%), Query Frame = 1 
Query:    4 KTGDNLRKTLLAGKVEVYDMVGKMTNCNGGGQCGTCAVDVVEAEGWDP-RSEWEAGKLK 177
            + G NLRK LLA  VEVY  + K+TNCNGGGQCGTCAV+VV  EG  P RSEWE GKL+
Sbjct:  178 QAGANLRKELLANGVEVYAGMAKLTNCNGGGQCGTCAVEVVAGEGLGPARSEWEEGKLR 236
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BLAST of mRNA_E-fasciculatus_F_contig13357.2518.1 vs. uniprot
Match: A0A4D9D0U6_9STRA (2Fe-2S ferredoxin-type domain-containing protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9D0U6_9STRA)

HSP 1 Score: 79.0 bits (193), Expect = 7.100e-17
Identity = 37/58 (63.79%), Postives = 43/58 (74.14%), Query Frame = 1 
Query:    1 AKTGDNLRKTLLAGKVEVYDMVGKMTNCNGGGQCGTCAVDVVEAEGWDPRSEWEAGKL 174
            AK G NLRKTL A K++VYD++GKMTNCNG GQCGTC V V     W  RSE+EA K+
Sbjct:   90 AKPGSNLRKTLQASKIDVYDLMGKMTNCNGAGQCGTCVVKVTSPS-WGLRSEYEADKI 146
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BLAST of mRNA_E-fasciculatus_F_contig13357.2518.1 vs. uniprot
Match: A0A7S1BW89_9STRA (Hypothetical protein n=1 Tax=Corethron hystrix TaxID=216773 RepID=A0A7S1BW89_9STRA)

HSP 1 Score: 72.0 bits (175), Expect = 7.050e-15
Identity = 35/59 (59.32%), Postives = 43/59 (72.88%), Query Frame = 1 
Query:    1 AKTGDNLRKTLLAGKVEVY-DMVGKMTNCNGGGQCGTCAVDVVEAEGWDPRSEWEAGKL 174
            A+ GDNLRKTLL  KVE+Y  +  K+ NC GGGQC  CAVD+VE +GW  RSE+E  K+
Sbjct:   30 ARVGDNLRKTLLENKVELYRGLKKKLGNCGGGGQCTFCAVDMVETDGWLERSEYEDSKI 88
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BLAST of mRNA_E-fasciculatus_F_contig13357.2518.1 vs. uniprot
Match: A0A1E7EPR9_9STRA (Uncharacterized protein n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7EPR9_9STRA)

HSP 1 Score: 72.4 bits (176), Expect = 6.830e-14
Identity = 33/58 (56.90%), Postives = 41/58 (70.69%), Query Frame = 1 
Query:    4 KTGDNLRKTLLAGKVEVYD-MVGKMTNCNGGGQCGTCAVDVVEAEGWDPRSEWEAGKL 174
            K GDNLR TLL   +EVY  M  K+ NC GGGQCG CA D +++EGW+PR+E+E  K 
Sbjct:  166 KVGDNLRTTLLDNNIEVYKGMKQKLGNCGGGGQCGFCAADFIDSEGWEPRNEYEDKKF 223
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BLAST of mRNA_E-fasciculatus_F_contig13357.2518.1 vs. uniprot
Match: A0A098TL89_9CYAN (Iron ABC transporter substrate-binding protein n=1 Tax=Neosynechococcus sphagnicola sy1 TaxID=1497020 RepID=A0A098TL89_9CYAN)

HSP 1 Score: 68.2 bits (165), Expect = 1.670e-13
Identity = 35/62 (56.45%), Postives = 41/62 (66.13%), Query Frame = 1 
Query:    1 AKTGDNLRKTLLAGKVEVYDMVGKMTNCNGGGQCGTCAVDVVE-AEGWDPRSEWEAGKLKVK 183
            A  G NLR   L   V++Y  VGKMTNC G GQCGTC V++VE AE   PRS++E  KLK K
Sbjct:   15 AADGANLRLQALENGVDIYTFVGKMTNCGGYGQCGTCVVEIVEGAENLSPRSDFETRKLKKK 76
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BLAST of mRNA_E-fasciculatus_F_contig13357.2518.1 vs. uniprot
Match: A0A7S1E0B0_9STRA (Hypothetical protein n=1 Tax=Thalassionema nitzschioides TaxID=33649 RepID=A0A7S1E0B0_9STRA)

HSP 1 Score: 69.3 bits (168), Expect = 2.480e-13
Identity = 34/59 (57.63%), Postives = 42/59 (71.19%), Query Frame = 1 
Query:    1 AKTGDNLRKTLLAGKVEVY-DMVGKMTNCNGGGQCGTCAVDVVEAEGWDPRSEWEAGKL 174
            A+ GDNLRK LL   VE+Y  +  K+ NC GGGQC  CAVD VE+EGW+ RSE+E  K+
Sbjct:   79 AQVGDNLRKLLLENDVELYRGLKKKLGNCGGGGQCTFCAVDFVESEGWEERSEYEDKKI 137
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BLAST of mRNA_E-fasciculatus_F_contig13357.2518.1 vs. uniprot
Match: A0A7S2GWY2_9STRA (Hypothetical protein n=1 Tax=Helicotheca tamesis TaxID=374047 RepID=A0A7S2GWY2_9STRA)

HSP 1 Score: 68.2 bits (165), Expect = 3.440e-12
Identity = 32/59 (54.24%), Postives = 41/59 (69.49%), Query Frame = 1 
Query:    1 AKTGDNLRKTLLAGKVEVY-DMVGKMTNCNGGGQCGTCAVDVVEAEGWDPRSEWEAGKL 174
            A+ GDNLRK LL   VE+Y  +  K+ NC GGGQC  CAVD V+ EGW+ RSE+E  ++
Sbjct:  187 ARVGDNLRKKLLENNVELYRGLKKKLGNCGGGGQCTYCAVDFVDGEGWEERSEYEDSRI 245
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BLAST of mRNA_E-fasciculatus_F_contig13357.2518.1 vs. uniprot
Match: A0A7S2W1W1_9STRA (Hypothetical protein (Fragment) n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2W1W1_9STRA)

HSP 1 Score: 67.8 bits (164), Expect = 4.080e-12
Identity = 32/59 (54.24%), Postives = 42/59 (71.19%), Query Frame = 1 
Query:    1 AKTGDNLRKTLLAGKVEVY-DMVGKMTNCNGGGQCGTCAVDVVEAEGWDPRSEWEAGKL 174
            AK G+NLRKTLL  KVEVY  +  K+ NC G G C  CAVD V++EGW+ RS++E  ++
Sbjct:  177 AKVGNNLRKTLLENKVEVYRGLKKKLGNCGGSGTCAFCAVDFVDSEGWEKRSDYEEKRI 235
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig13357.2518.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LDD4_ECTSI1.210e-3396.61PDZ domain-containing protein n=2 Tax=Ectocarpus T... [more]
A0A836CP36_9STRA9.780e-1862.712Fe-2S ferredoxin-type domain-containing protein n... [more]
A0A7S3YA98_HETAK5.400e-1767.80Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A4D9D0U6_9STRA7.100e-1763.792Fe-2S ferredoxin-type domain-containing protein n... [more]
A0A7S1BW89_9STRA7.050e-1559.32Hypothetical protein n=1 Tax=Corethron hystrix Tax... [more]
A0A1E7EPR9_9STRA6.830e-1456.90Uncharacterized protein n=1 Tax=Fragilariopsis cyl... [more]
A0A098TL89_9CYAN1.670e-1356.45Iron ABC transporter substrate-binding protein n=1... [more]
A0A7S1E0B0_9STRA2.480e-1357.63Hypothetical protein n=1 Tax=Thalassionema nitzsch... [more]
A0A7S2GWY2_9STRA3.440e-1254.24Hypothetical protein n=1 Tax=Helicotheca tamesis T... [more]
A0A7S2W1W1_9STRA4.080e-1254.24Hypothetical protein (Fragment) n=1 Tax=Eucampia a... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig13357contigE-fasciculatus_F_contig13357:1177..2161 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score123.6
Seed ortholog evalue3.1e-26
Seed eggNOG ortholog2880.D8LDD4
EggNOG free text desc.Domain present in PSD-95, Dlg, and ZO-1/2.
EggNOG OGs2DJ3Z@1,2S5ZV@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionPDZ domain
Ec32 orthologEc-25_001670.1
Exons2
Model size183
Cds size183
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927836.811178-CDS-E-fasciculatus_F_contig13357:1176..12481622927836.811178-CDS-E-fasciculatus_F_contig13357:1176..1248Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig13357 1177..1248 +
1695995716.3399684-CDS-E-fasciculatus_F_contig13357:1176..12481695995716.3399684-CDS-E-fasciculatus_F_contig13357:1176..1248Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig13357 1177..1248 +
1622927836.8258631-CDS-E-fasciculatus_F_contig13357:2050..21611622927836.8258631-CDS-E-fasciculatus_F_contig13357:2050..2161Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig13357 2051..2161 +
1695995716.3589892-CDS-E-fasciculatus_F_contig13357:2050..21611695995716.3589892-CDS-E-fasciculatus_F_contig13357:2050..2161Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig13357 2051..2161 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig13357.2518.1prot_E-fasciculatus_F_contig13357.2518.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig13357 1177..2161 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig13357.2518.1

>prot_E-fasciculatus_F_contig13357.2518.1 ID=prot_E-fasciculatus_F_contig13357.2518.1|Name=mRNA_E-fasciculatus_F_contig13357.2518.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=61bp
AKTGDNLRKTLLAGKVEVYDMVGKMTNCNGGGQCGTCAVDVVEAEGWDPR
SEWEAGKLKVK
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mRNA from alignment at E-fasciculatus_F_contig13357:1177..2161+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig13357.2518.1 ID=mRNA_E-fasciculatus_F_contig13357.2518.1|Name=mRNA_E-fasciculatus_F_contig13357.2518.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=985bp|location=Sequence derived from alignment at E-fasciculatus_F_contig13357:1177..2161+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
GCCAAGACGGGGGATAACCTAAGGAAGACGCTCCTCGCGGGGAAAGTGGA
GGTGTACGACATGGTCGGGAAGGTAGGTTTCTTTTTTCGCGTTTTGGGTG
CACGATCGGCGGAAAAAAATATCGCGACTCTCTCGCGTTTTCTGCTCCAA
TCCCCTAATCTGGCGAAAAACCCGGCATATCGCTCCGTTTCCGGTAGCAT
TTTAGGGACACGAGGTCCATGGTCTAACGACTACAGCATGCGTTTACATG
AATACCCCCCATTAGCCTGGTGCGGTGAAAACCCGGCGTGCTCCGTCCCG
GCTTTGTCGGGATGCGACGTCGAATATGGTGTAACGTCTATGCACACGGC
GTGCGTTTACGGGAGCACACCACCCCCTTCTCCCCATTTTTTTTGTCGTT
CCCCCTCTCTGTGTGTCAACAGAGAGGGTTACGTAGCGGTCCAGGGCGTG
TTTTTCGTTGAGTCTTGTGAACCACAGAACGAGACAATGATTCAGGCGGC
GCTTTGGCGCTTGTAGCTGCGACAGATCGGGATGGACGCACTAACGACAA
TTCTGAACGAAGTTTTAGTACAATCGTCTTGCGGGGCATGCACGTGTTTC
ACTACTCCAGACGCGGTGTTTTCGTGGAGAACGGTGAATTTTCCAGTACA
GTACCACGAATTGCACCAATACTGTAGCAGTAGGGTTCACCTCAACTAGT
AGTACTTGTTACTGTACTGTTGTCCGAGCAAGATTGAGGAACTGGGAGAA
ATTGCTGAACCAAAAGAAGAACATACACGGCAAGCAACAGTAGTACCACA
CACATGCAACATTCAGACAGCAACTAAAACTGCCGTGCTTTCTGCATTCC
GATTCCGCGTAAACTATCCATCAGATGACCAACTGCAACGGAGGAGGTCA
GTGCGGCACGTGCGCTGTAGACGTTGTCGAAGCCGAAGGCTGGGATCCCA
GGAGCGAGTGGGAAGCTGGCAAGCTCAAGGTGAAG
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig13357:1177..2161+

>mRNA_E-fasciculatus_F_contig13357.2518.1 ID=mRNA_E-fasciculatus_F_contig13357.2518.1|Name=mRNA_E-fasciculatus_F_contig13357.2518.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=366bp|location=Sequence derived from alignment at E-fasciculatus_F_contig13357:1177..2161+ (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
GCCAAGACGGGGGATAACCTAAGGAAGACGCTCCTCGCGGGGAAAGTGGA
GGTGTACGACATGGTCGGGAAGGCCAAGACGGGGGATAACCTAAGGAAGA
CGCTCCTCGCGGGGAAAGTGGAGGTGTACGACATGGTCGGGAAGATGACC
AACTGCAACGGAGGAGGTCAGTGCGGCACGTGCGCTGTAGACGTTGTCGA
AGCCGAAGGCTGGGATCCCAGGAGCGAGTGGGAAGCTGGCAAGCTCAAGG
TGAAGATGACCAACTGCAACGGAGGAGGTCAGTGCGGCACGTGCGCTGTA
GACGTTGTCGAAGCCGAAGGCTGGGATCCCAGGAGCGAGTGGGAAGCTGG
CAAGCTCAAGGTGAAG
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