mRNA_E-fasciculatus_F_contig13354.2516.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig13354.2516.1
Unique NamemRNA_E-fasciculatus_F_contig13354.2516.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig13354.2516.1 vs. uniprot
Match: D7G723_ECTSI (DNA topoisomerase I n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G723_ECTSI)

HSP 1 Score: 68.2 bits (165), Expect = 1.200e-13
Identity = 34/41 (82.93%), Postives = 35/41 (85.37%), Query Frame = 1 
Query:    1 FTLIPVIWNFVPPGLSAGRVQSVALAMVVEPECARLKFKSA 123
            FTL PVIWNFV PGLSAGRVQSVA AMV+E ECARLK  SA
Sbjct:   66 FTLTPVIWNFVAPGLSAGRVQSVAPAMVIERECARLKSNSA 106
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BLAST of mRNA_E-fasciculatus_F_contig13354.2516.1 vs. uniprot
Match: D8LDU2_ECTSI (DNA topoisomerase (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LDU2_ECTSI)

HSP 1 Score: 71.6 bits (174), Expect = 1.690e-13
Identity = 37/41 (90.24%), Postives = 37/41 (90.24%), Query Frame = 1 
Query:    1 FTLIPVIWNFVPPGLSAGRVQSVALAMVVEPECARLKFKSA 123
            FTL PVIWNFV PGLSAGRVQSVALAMVVE E ARLKFKSA
Sbjct:  261 FTLTPVIWNFVAPGLSAGRVQSVALAMVVERERARLKFKSA 301
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BLAST of mRNA_E-fasciculatus_F_contig13354.2516.1 vs. uniprot
Match: A0A7V5M4C3_9BACT (DNA topoisomerase 1 n=2 Tax=cellular organisms TaxID=131567 RepID=A0A7V5M4C3_9BACT)

HSP 1 Score: 58.2 bits (139), Expect = 9.470e-9
Identity = 27/41 (65.85%), Postives = 34/41 (82.93%), Query Frame = 1 
Query:    1 FTLIPVIWNFVPPGLSAGRVQSVALAMVVEPECARLKFKSA 123
            +TL P++W  + PGLSAGRVQSVALAM+VE E  RL+F+SA
Sbjct:  367 YTLSPLLWKKIAPGLSAGRVQSVALAMIVERELERLRFRSA 407
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BLAST of mRNA_E-fasciculatus_F_contig13354.2516.1 vs. uniprot
Match: A0A7J7IIF0_9RHOD (Toprim domain-containing protein n=1 Tax=Cyanidiococcus yangmingshanensis TaxID=2690220 RepID=A0A7J7IIF0_9RHOD)

HSP 1 Score: 57.0 bits (136), Expect = 2.410e-8
Identity = 26/41 (63.41%), Postives = 34/41 (82.93%), Query Frame = 1 
Query:    1 FTLIPVIWNFVPPGLSAGRVQSVALAMVVEPECARLKFKSA 123
            +TL P++W  + PGLSAGRVQSVAL+M+VE E  RL+F+SA
Sbjct:  298 YTLSPLLWKKIAPGLSAGRVQSVALSMIVERELERLRFRSA 338
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BLAST of mRNA_E-fasciculatus_F_contig13354.2516.1 vs. uniprot
Match: A0A835Z3T3_9STRA (DNA topoisomerase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z3T3_9STRA)

HSP 1 Score: 56.6 bits (135), Expect = 3.310e-8
Identity = 26/41 (63.41%), Postives = 34/41 (82.93%), Query Frame = 1 
Query:    1 FTLIPVIWNFVPPGLSAGRVQSVALAMVVEPECARLKFKSA 123
            +T+ P++W  + P LSAGRVQSVALAM+++ E ARLKFKSA
Sbjct:  520 YTMSPLLWRKIAPKLSAGRVQSVALAMIIKREMARLKFKSA 560
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BLAST of mRNA_E-fasciculatus_F_contig13354.2516.1 vs. uniprot
Match: A0A521DC44_9BACT (DNA topoisomerase 1 n=1 Tax=Gracilimonas mengyeensis TaxID=1302730 RepID=A0A521DC44_9BACT)

HSP 1 Score: 52.0 bits (123), Expect = 1.420e-6
Identity = 24/41 (58.54%), Postives = 32/41 (78.05%), Query Frame = 1 
Query:    1 FTLIPVIWNFVPPGLSAGRVQSVALAMVVEPECARLKFKSA 123
            +T+ P++W  + PGLSAGRVQSVA+  +VE E  R+KFKSA
Sbjct:  160 YTVSPLLWKKISPGLSAGRVQSVAVEFLVERERERMKFKSA 200
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BLAST of mRNA_E-fasciculatus_F_contig13354.2516.1 vs. uniprot
Match: A0A1B6Y878_9BACT (DNA topoisomerase 1 n=7 Tax=Bacteroidetes/Chlorobi group TaxID=68336 RepID=A0A1B6Y878_9BACT)

HSP 1 Score: 51.6 bits (122), Expect = 1.940e-6
Identity = 23/40 (57.50%), Postives = 32/40 (80.00%), Query Frame = 1 
Query:    1 FTLIPVIWNFVPPGLSAGRVQSVALAMVVEPECARLKFKS 120
            +T+ P++W  + PGLSAGRVQSVA+ M+VE E  R+KF+S
Sbjct:  160 YTVSPLLWKKISPGLSAGRVQSVAVKMLVERERERMKFRS 199
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BLAST of mRNA_E-fasciculatus_F_contig13354.2516.1 vs. uniprot
Match: M2XMQ1_GALSU (DNA topoisomerase n=1 Tax=Galdieria sulphuraria TaxID=130081 RepID=M2XMQ1_GALSU)

HSP 1 Score: 51.2 bits (121), Expect = 2.660e-6
Identity = 23/39 (58.97%), Postives = 30/39 (76.92%), Query Frame = 1 
Query:    1 FTLIPVIWNFVPPGLSAGRVQSVALAMVVEPECARLKFK 117
            FTL P++W  + P LSAGRVQSVALAM+V+ E  R+ F+
Sbjct:  264 FTLSPILWKKIAPRLSAGRVQSVALAMIVQRELERITFR 302
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BLAST of mRNA_E-fasciculatus_F_contig13354.2516.1 vs. uniprot
Match: A0A2A2GBY7_9BACT (DNA topoisomerase 1 n=1 Tax=Aliifodinibius sp. WN023 TaxID=2032627 RepID=A0A2A2GBY7_9BACT)

HSP 1 Score: 51.2 bits (121), Expect = 2.660e-6
Identity = 23/41 (56.10%), Postives = 32/41 (78.05%), Query Frame = 1 
Query:    1 FTLIPVIWNFVPPGLSAGRVQSVALAMVVEPECARLKFKSA 123
            +T+ P++W  + PGLSAGRVQSVA+  +VE E  R+KF+SA
Sbjct:  160 YTISPLLWKKIAPGLSAGRVQSVAVRFLVERERERMKFRSA 200
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BLAST of mRNA_E-fasciculatus_F_contig13354.2516.1 vs. uniprot
Match: A0A5D3YIY6_9BACT (DNA topoisomerase 1 n=1 Tax=Fodinibius salinus TaxID=860790 RepID=A0A5D3YIY6_9BACT)

HSP 1 Score: 51.2 bits (121), Expect = 2.660e-6
Identity = 23/41 (56.10%), Postives = 32/41 (78.05%), Query Frame = 1 
Query:    1 FTLIPVIWNFVPPGLSAGRVQSVALAMVVEPECARLKFKSA 123
            +T+ P++W  + PGLSAGRVQSVA+  +VE E  R+KF+SA
Sbjct:  160 YTISPLLWKKIAPGLSAGRVQSVAVRFLVERERERMKFRSA 200
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig13354.2516.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G723_ECTSI1.200e-1382.93DNA topoisomerase I n=1 Tax=Ectocarpus siliculosus... [more]
D8LDU2_ECTSI1.690e-1390.24DNA topoisomerase (Fragment) n=1 Tax=Ectocarpus si... [more]
A0A7V5M4C3_9BACT9.470e-965.85DNA topoisomerase 1 n=2 Tax=cellular organisms Tax... [more]
A0A7J7IIF0_9RHOD2.410e-863.41Toprim domain-containing protein n=1 Tax=Cyanidioc... [more]
A0A835Z3T3_9STRA3.310e-863.41DNA topoisomerase n=1 Tax=Tribonema minus TaxID=30... [more]
A0A521DC44_9BACT1.420e-658.54DNA topoisomerase 1 n=1 Tax=Gracilimonas mengyeens... [more]
A0A1B6Y878_9BACT1.940e-657.50DNA topoisomerase 1 n=7 Tax=Bacteroidetes/Chlorobi... [more]
M2XMQ1_GALSU2.660e-658.97DNA topoisomerase n=1 Tax=Galdieria sulphuraria Ta... [more]
A0A2A2GBY7_9BACT2.660e-656.10DNA topoisomerase 1 n=1 Tax=Aliifodinibius sp. WN0... [more]
A0A5D3YIY6_9BACT2.660e-656.10DNA topoisomerase 1 n=1 Tax=Fodinibius salinus Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig13354contigE-fasciculatus_F_contig13354:3522..3644 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score71.6
Seed ortholog evalue9.4e-11
Seed eggNOG ortholog2880.D8LDU2
KEGG koko:K03168
EggNOG free text desc.DNA topoisomerase type I activity
EggNOG OGsCOG0550@1,KOG1956@2759
EC5.99.1.2
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03032,ko03400
Hectar predicted targeting categoryno signal peptide or anchor
Ec32 ortholog descriptionDNA topoisomerase, type IA, central
Ec32 orthologEc-01_011320.1
Exons1
Model size123
Cds size123
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927836.6912928-CDS-E-fasciculatus_F_contig13354:3521..36441622927836.6912928-CDS-E-fasciculatus_F_contig13354:3521..3644Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig13354 3522..3644 -
1695995716.2734725-CDS-E-fasciculatus_F_contig13354:3521..36441695995716.2734725-CDS-E-fasciculatus_F_contig13354:3521..3644Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig13354 3522..3644 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig13354.2516.1prot_E-fasciculatus_F_contig13354.2516.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig13354 3522..3644 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig13354.2516.1

>prot_E-fasciculatus_F_contig13354.2516.1 ID=prot_E-fasciculatus_F_contig13354.2516.1|Name=mRNA_E-fasciculatus_F_contig13354.2516.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=41bp
FTLIPVIWNFVPPGLSAGRVQSVALAMVVEPECARLKFKSA
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mRNA from alignment at E-fasciculatus_F_contig13354:3522..3644-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig13354.2516.1 ID=mRNA_E-fasciculatus_F_contig13354.2516.1|Name=mRNA_E-fasciculatus_F_contig13354.2516.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=123bp|location=Sequence derived from alignment at E-fasciculatus_F_contig13354:3522..3644- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
TTTACCCTGATCCCGGTCATCTGGAACTTCGTCCCTCCCGGGCTTAGCGC
CGGACGGGTGCAGAGCGTAGCGCTGGCGATGGTGGTCGAGCCCGAGTGCG
CGCGCCTCAAGTTCAAGTCCGCG
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig13354:3522..3644-

>mRNA_E-fasciculatus_F_contig13354.2516.1 ID=mRNA_E-fasciculatus_F_contig13354.2516.1|Name=mRNA_E-fasciculatus_F_contig13354.2516.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=246bp|location=Sequence derived from alignment at E-fasciculatus_F_contig13354:3522..3644- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
TTTACCCTGATCCCGGTCATCTGGAACTTCGTCCCTCCCGGGCTTAGCGC
CGGACGGGTGCAGAGCGTAGCGCTGGCGATGGTGGTCGAGCCCGAGTGCG
CGCGCCTCAAGTTCAAGTCCGCGTTTACCCTGATCCCGGTCATCTGGAAC
TTCGTCCCTCCCGGGCTTAGCGCCGGACGGGTGCAGAGCGTAGCGCTGGC
GATGGTGGTCGAGCCCGAGTGCGCGCGCCTCAAGTTCAAGTCCGCG
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