mRNA_D-mesarthrocarpus_Contig11249.1.1 (mRNA) Discosporangium mesarthrocarpum MT17_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_D-mesarthrocarpus_Contig11249.1.1
Unique NamemRNA_D-mesarthrocarpus_Contig11249.1.1
TypemRNA
OrganismDiscosporangium mesarthrocarpum MT17_79 (Discosporangium mesarthrocarpum MT17_79)
Homology
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BLAST of mRNA_D-mesarthrocarpus_Contig11249.1.1 vs. uniprot
Match: D7FPP9_ECTSI (Alanine--tRNA ligase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FPP9_ECTSI)

HSP 1 Score: 101 bits (252), Expect = 5.260e-23
Identity = 56/91 (61.54%), Postives = 66/91 (72.53%), Query Frame = 1 
Query:    1 PQVVRIMKVIFPEVVEAEARVVEVLLDEETTFNRTLDKGIKEFNKIADGLVDSNSPLFCFPARVVSGEAAFFLYSTMGFPLDLTALMAEEK 273
            P VV +MK IFPE+V+AEARV  V+ DEE +FN+TLDKG+KEFNK+A  L DS         +V  G AAF LYS+MGFPLDL  LMAEEK
Sbjct:  377 PVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAALGDS---------KVFPGTAAFQLYSSMGFPLDLLTLMAEEK 458
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BLAST of mRNA_D-mesarthrocarpus_Contig11249.1.1 vs. uniprot
Match: A0A6H5L0C8_9PHAE (Alanine--tRNA ligase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L0C8_9PHAE)

HSP 1 Score: 100 bits (248), Expect = 1.800e-22
Identity = 55/91 (60.44%), Postives = 65/91 (71.43%), Query Frame = 1 
Query:    1 PQVVRIMKVIFPEVVEAEARVVEVLLDEETTFNRTLDKGIKEFNKIADGLVDSNSPLFCFPARVVSGEAAFFLYSTMGFPLDLTALMAEEK 273
            P VV +MK IFPE+V+AEARV  V+ DEE +FN+TLDKG+KEFNK+A  L DS         +V  G  AF LYS+MGFPLDL  LMAEEK
Sbjct:  266 PVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVAAALGDS---------KVFPGTVAFQLYSSMGFPLDLLTLMAEEK 347
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BLAST of mRNA_D-mesarthrocarpus_Contig11249.1.1 vs. uniprot
Match: A0A8K1CQN1_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CQN1_PYTOL)

HSP 1 Score: 91.7 bits (226), Expect = 1.710e-19
Identity = 50/98 (51.02%), Postives = 64/98 (65.31%), Query Frame = 1 
Query:    1 PQVVRIMKVIFPEVVEAEARVVEVLLDEETTFNRTLDKGIKEFNKIADGLVDSNSPLFCFPARVVSGEAAFFLYSTMGFPLDLTALMAEEKVWYFSLP 294
            P VV ++   FPE+   EA V EV+LDEE +F RTL+KGI  F KIA GL +++        +VV GE AFFLY +MGFPLDLT +MAEE+     +P
Sbjct:  358 PVVVSMLSEAFPELTAKEAEVKEVILDEEKSFGRTLNKGIDRFKKIAQGLKEADK-------KVVPGEDAFFLYDSMGFPLDLTQIMAEEQGMTVDVP 448
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BLAST of mRNA_D-mesarthrocarpus_Contig11249.1.1 vs. uniprot
Match: A0A484EK79_BRELC (Alanine--tRNA ligase n=1 Tax=Bremia lactucae TaxID=4779 RepID=A0A484EK79_BRELC)

HSP 1 Score: 90.5 bits (223), Expect = 4.350e-19
Identity = 46/91 (50.55%), Postives = 66/91 (72.53%), Query Frame = 1 
Query:    1 PQVVRIMKVIFPEVVEAEARVVEVLLDEETTFNRTLDKGIKEFNKIADGLVDSNSPLFCFPARVVSGEAAFFLYSTMGFPLDLTALMAEEK 273
            P VV ++   FPE+++ +A V E++LDEE +F RTL+KGI+ F KIA G+ ++N+      A +V GE AFFLY +MGFP+DLT +MAEE+
Sbjct:  388 PVVVTMLGEAFPELLQKQAEVEEIILDEEHSFGRTLNKGIERFKKIASGIKEANAG--SNEALIVPGEDAFFLYDSMGFPIDLTEIMAEEE 476
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BLAST of mRNA_D-mesarthrocarpus_Contig11249.1.1 vs. uniprot
Match: A0A024UCS6_9STRA (Alanine--tRNA ligase n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024UCS6_9STRA)

HSP 1 Score: 89.0 bits (219), Expect = 1.510e-18
Identity = 48/91 (52.75%), Postives = 61/91 (67.03%), Query Frame = 1 
Query:    1 PQVVRIMKVIFPEVVEAEARVVEVLLDEETTFNRTLDKGIKEFNKIADGLVDSNSPLFCFPARVVSGEAAFFLYSTMGFPLDLTALMAEEK 273
            P+VV +M   FPE+V  +++V+EV+LDEE +F RTL+KG+  F KIA  L             VV GE AFFLY +MGFPLDLT LMAEE+
Sbjct:  366 PKVVEMMGAAFPELVTKQSQVIEVILDEEESFGRTLNKGLDRFKKIAAALKAEKK-------TVVPGEDAFFLYDSMGFPLDLTQLMAEEQ 449
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BLAST of mRNA_D-mesarthrocarpus_Contig11249.1.1 vs. uniprot
Match: A0A2D4BS21_PYTIN (Alanine--tRNA ligase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BS21_PYTIN)

HSP 1 Score: 89.0 bits (219), Expect = 1.550e-18
Identity = 48/91 (52.75%), Postives = 61/91 (67.03%), Query Frame = 1 
Query:    1 PQVVRIMKVIFPEVVEAEARVVEVLLDEETTFNRTLDKGIKEFNKIADGLVDSNSPLFCFPARVVSGEAAFFLYSTMGFPLDLTALMAEEK 273
            P VV ++   FPE+   EA V EV+LDEE +F RTL+KGI+ F KIA  + D+   L      VV GE AFFLY +MGFP+DLT +MAEE+
Sbjct: 1077 PVVVGMLSEAFPELAAKEATVKEVILDEEQSFGRTLNKGIERFKKIAQAIKDNKGEL------VVPGEDAFFLYDSMGFPIDLTQIMAEEE 1161
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BLAST of mRNA_D-mesarthrocarpus_Contig11249.1.1 vs. uniprot
Match: A0A1W0A011_9STRA (Alanine--tRNA ligase n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1W0A011_9STRA)

HSP 1 Score: 88.6 bits (218), Expect = 2.060e-18
Identity = 45/91 (49.45%), Postives = 61/91 (67.03%), Query Frame = 1 
Query:    1 PQVVRIMKVIFPEVVEAEARVVEVLLDEETTFNRTLDKGIKEFNKIADGLVDSNSPLFCFPARVVSGEAAFFLYSTMGFPLDLTALMAEEK 273
            P VV ++   FPE+ E +A V+E++LDEE +F RTL+KG++ F KIA  +             VVSGE AFFLY +MGFP+DLT +MAEE+
Sbjct:  367 PVVVEMLSSAFPELKEKQAHVIEIILDEEVSFGRTLNKGLERFKKIASAMKTEKK-------TVVSGEDAFFLYDSMGFPVDLTEIMAEEQ 450
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BLAST of mRNA_D-mesarthrocarpus_Contig11249.1.1 vs. uniprot
Match: W4FJR7_9STRA (Alanine--tRNA ligase n=11 Tax=Aphanomyces astaci TaxID=112090 RepID=W4FJR7_9STRA)

HSP 1 Score: 88.2 bits (217), Expect = 2.820e-18
Identity = 47/91 (51.65%), Postives = 60/91 (65.93%), Query Frame = 1 
Query:    1 PQVVRIMKVIFPEVVEAEARVVEVLLDEETTFNRTLDKGIKEFNKIADGLVDSNSPLFCFPARVVSGEAAFFLYSTMGFPLDLTALMAEEK 273
            P+VV ++   FPE++  +A+V+EV+LDEE +F RTL KG+  F KIA  L             VV GE AFFLY +MGFPLDLT LMAEE+
Sbjct:  388 PKVVEMLGGAFPELISKQAQVIEVILDEEESFGRTLSKGLDRFKKIAAALKSEKK-------TVVPGEDAFFLYDSMGFPLDLTQLMAEEQ 471
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BLAST of mRNA_D-mesarthrocarpus_Contig11249.1.1 vs. uniprot
Match: W7TIB5_9STRA (Alanine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TIB5_9STRA)

HSP 1 Score: 87.8 bits (216), Expect = 3.840e-18
Identity = 54/96 (56.25%), Postives = 62/96 (64.58%), Query Frame = 1 
Query:    7 VVRIMKVIFPEVVEAEARVVEVLLDEETTFNRTLDKGIKEFNKIADGLVDSNSPL-------FCFPARVVSGEAAFFLYSTMGFPLDLTALMAEEK 273
            VV  MK IFPE+ E E RVVEVL DEE  FNRTLD+G K F K+AD L+ + S           FPA+      A +LYSTMGFP+DLT LMAEEK
Sbjct:  374 VVNQMKDIFPELEEKEKRVVEVLADEERAFNRTLDQGEKHFKKVADELLLAASQRGGDKGEKVAFPAK-----EAHYLYSTMGFPVDLTELMAEEK 464
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BLAST of mRNA_D-mesarthrocarpus_Contig11249.1.1 vs. uniprot
Match: A0A5D6XGK8_9STRA (Alanine--tRNA ligase n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XGK8_9STRA)

HSP 1 Score: 87.8 bits (216), Expect = 3.840e-18
Identity = 51/92 (55.43%), Postives = 62/92 (67.39%), Query Frame = 1 
Query:    1 PQVVRIMKVIFPEVVEAEARVVEVLLDEETTFNRTLDKGIKEFNKIADGLVDSNS--PLFCFPARVVSGEAAFFLYSTMGFPLDLTALMAEE 270
            P VV ++   FPE+   +A V EV+LDEE +F RTL+KGI+ F KIA G+ +S    PL      VV GE AFFLY +MGFPLDLT LMAEE
Sbjct:  382 PVVVAMLAGAFPELAAKQAAVQEVILDEEQSFGRTLNKGIERFKKIAQGIRESGGAGPL------VVPGEDAFFLYDSMGFPLDLTQLMAEE 467
The following BLAST results are available for this feature:
BLAST of mRNA_D-mesarthrocarpus_Contig11249.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FPP9_ECTSI5.260e-2361.54Alanine--tRNA ligase n=1 Tax=Ectocarpus siliculosu... [more]
A0A6H5L0C8_9PHAE1.800e-2260.44Alanine--tRNA ligase n=1 Tax=Ectocarpus sp. CCAP 1... [more]
A0A8K1CQN1_PYTOL1.710e-1951.02Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A484EK79_BRELC4.350e-1950.55Alanine--tRNA ligase n=1 Tax=Bremia lactucae TaxID... [more]
A0A024UCS6_9STRA1.510e-1852.75Alanine--tRNA ligase n=2 Tax=Aphanomyces invadans ... [more]
A0A2D4BS21_PYTIN1.550e-1852.75Alanine--tRNA ligase n=1 Tax=Pythium insidiosum Ta... [more]
A0A1W0A011_9STRA2.060e-1849.45Alanine--tRNA ligase n=1 Tax=Thraustotheca clavata... [more]
W4FJR7_9STRA2.820e-1851.65Alanine--tRNA ligase n=11 Tax=Aphanomyces astaci T... [more]
W7TIB5_9STRA3.840e-1856.25Alanine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID... [more]
A0A5D6XGK8_9STRA3.840e-1855.43Alanine--tRNA ligase n=1 Tax=Pythium brassicum Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
D-mesarthrocarpus_Contig11249contigD-mesarthrocarpus_Contig11249:2282..3077 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Discosporangium mesarthrocarpum MT17_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog4787.PITG_12961T0
PFAMsDHHA1,tRNA-synt_2c,tRNA_SAD
Model size294
Max annot lvl4776|Peronosporales
KEGG rclassRC00055,RC00523
KEGG koko:K01872
KEGG ReactionR03038
KEGG Pathwayko00970,map00970
KEGG ModuleM00359,M00360
Hectar predicted targeting categoryother localisation
Exons2
Evalue2.36e-18
EggNOG OGsCOG0013@1|root,KOG0188@2759|Eukaryota,3Q7RJ@4776|Peronosporales
EC6.1.1.7
DescriptionCatalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain
Cds size276
COG categoryJ
BRITEko00000,ko00001,ko00002,ko01000,ko01007,ko03016
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789247.8220096-CDS-D-mesarthrocarpus_Contig11249:2281..24141680789247.8220096-CDS-D-mesarthrocarpus_Contig11249:2281..2414Discosporangium mesarthrocarpum MT17_79CDSD-mesarthrocarpus_Contig11249 2282..2414 -
1680789247.8368294-CDS-D-mesarthrocarpus_Contig11249:2916..30591680789247.8368294-CDS-D-mesarthrocarpus_Contig11249:2916..3059Discosporangium mesarthrocarpum MT17_79CDSD-mesarthrocarpus_Contig11249 2917..3059 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789247.8471546-UTR-D-mesarthrocarpus_Contig11249:3059..30771680789247.8471546-UTR-D-mesarthrocarpus_Contig11249:3059..3077Discosporangium mesarthrocarpum MT17_79UTRD-mesarthrocarpus_Contig11249 3060..3077 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_D-mesarthrocarpus_Contig11249.1.1prot_D-mesarthrocarpus_Contig11249.1.1Discosporangium mesarthrocarpum MT17_79polypeptideD-mesarthrocarpus_Contig11249 2282..3059 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_D-mesarthrocarpus_Contig11249.1.1

>prot_D-mesarthrocarpus_Contig11249.1.1 ID=prot_D-mesarthrocarpus_Contig11249.1.1|Name=mRNA_D-mesarthrocarpus_Contig11249.1.1|organism=Discosporangium mesarthrocarpum MT17_79|type=polypeptide|length=92bp
MKVIFPEVVEAEARVVEVLLDEETTFNRTLDKGIKEFNKIADGLVDSNSP
LFCFPARVVSGEAAFFLYSTMGFPLDLTALMAEEKVWYFSLP
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mRNA from alignment at D-mesarthrocarpus_Contig11249:2282..3077-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_D-mesarthrocarpus_Contig11249.1.1 ID=mRNA_D-mesarthrocarpus_Contig11249.1.1|Name=mRNA_D-mesarthrocarpus_Contig11249.1.1|organism=Discosporangium mesarthrocarpum MT17_79|type=mRNA|length=796bp|location=Sequence derived from alignment at D-mesarthrocarpus_Contig11249:2282..3077- (Discosporangium mesarthrocarpum MT17_79)
CCTCAGGTTGTCCGTATCATGAAGGTTATATTCCCTGAGGTGGTCGAAGC
GGAGGCCCGGGTAGTGGAGGTGCTCCTGGATGAAGAGACCACCTTCAACC
GCACCCTGGATAAGGGCATCAAGGAGTTCAACAAGATAGCCGATGGGCTG
GTGGACTCCAAGTGGGATACACATTGCATTGGCTTGCCCCCCCCCCCTGT
GTTAGGGTTAGGGTTAGGGTTACAGTGTTGAAGGTTATAGAGTTAGGCTT
AGGGTCCACGGGTTCGAGGGTCGCCCCCCATGCAATGAGCATGGCCTGCA
TCTAGGGTAGAGTTACAGGGTTGGGGTTAGGTTCAGGGGAAGAGGGTCTA
AGAATCGCCCTGCAGCCGAGCATGACTTGCATCTCCCATAAAATCAGTTG
ACTCGGGGATGTCACACGAATTGGCCTACGGTTAGGGCTGGGGTTGGGGT
CAATAAAAGTAGTAACATACATCAGGGATATCGTTATTAGGGGATGTGAG
GGTTAGGAGGGTGTGCTGTGGGTGAGAATTCACTTTGACCCCCTCCCCCA
CTCTGACCAGTGTTCCCCTGACCTGTCTCTGAGGATTTCACTCCTTGCTA
TAGTTAATTAAGAGACCCCCCCCCTGTCTCTTGGCGCCCGTCCTTCTCCA
TGGTGATTATGAGCTCCCCATTGTTTTGTTTTCCTGCCAGGGTTGTCTCC
GGTGAGGCTGCCTTCTTCTTGTACTCCACCATGGGCTTCCCCCTGGACCT
GACTGCCCTCATGGCCGAGGAGAAGGTGTGGTATTTCTCCCTCCCA
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Coding sequence (CDS) from alignment at D-mesarthrocarpus_Contig11249:2282..3077-

>mRNA_D-mesarthrocarpus_Contig11249.1.1 ID=mRNA_D-mesarthrocarpus_Contig11249.1.1|Name=mRNA_D-mesarthrocarpus_Contig11249.1.1|organism=Discosporangium mesarthrocarpum MT17_79|type=CDS|length=276bp|location=Sequence derived from alignment at D-mesarthrocarpus_Contig11249:2282..3077- (Discosporangium mesarthrocarpum MT17_79)
ATGAAGGTTATATTCCCTGAGGTGGTCGAAGCGGAGGCCCGGGTAGTGGA
GGTGCTCCTGGATGAAGAGACCACCTTCAACCGCACCCTGGATAAGGGCA
TCAAGGAGTTCAACAAGATAGCCGATGGGCTGGTGGACTCCAACTCCCCA
TTGTTTTGTTTTCCTGCCAGGGTTGTCTCCGGTGAGGCTGCCTTCTTCTT
GTACTCCACCATGGGCTTCCCCCTGGACCTGACTGCCCTCATGGCCGAGG
AGAAGGTGTGGTATTTCTCCCTCCCA
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