prot_D-mesarthrocarpus_Contig10624.3.1 (polypeptide) Discosporangium mesarthrocarpum MT17_79

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview
NamemRNA_D-mesarthrocarpus_Contig10624.3.1
Unique Nameprot_D-mesarthrocarpus_Contig10624.3.1
Typepolypeptide
OrganismDiscosporangium mesarthrocarpum MT17_79 (Discosporangium mesarthrocarpum MT17_79)
Sequence length230
Homology
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BLAST of mRNA_D-mesarthrocarpus_Contig10624.3.1 vs. uniprot
Match: A0A2E9JG77_9GAMM (Glycosyl transferase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A2E9JG77_9GAMM)

HSP 1 Score: 276 bits (706), Expect = 3.930e-91
Identity = 137/226 (60.62%), Postives = 171/226 (75.66%), Query Frame = 0 
Query:    1 MTHFSLIVPVLDEAAQIQQRLEPLQDLRQCGHEVIVVDGGSVDGSLPLAQSLADRILTVARGRARQMNAGARVASNEWLLFLHLDTELPESAAQIMANDIAVSGREWGWFDVQLANPGWPYRVIAWHMNRRARLTRVCTGDQALFVRRCCFEALGGFPDLPLMEDVALSKRLRRRSAPLVLSAPVTTSARRWEQHGVLRTVLLMWRLRLLYWLGVSPERLVNHYYP 226
            M   S+I+PVL+EAA I   L+ LQ +RQ G E++VVDGGS D ++ LA +LADR++    GRA+QMN GA +AS E LLFLHLD+ELPE A + +      +   WGWFDV+L++PG+ YRVIA  MN RARL+RVCTGDQALF+ R  FE +GGFP+LPLMEDVA+SKRLR+ S P+V   P+  S RRWE+ G+LRTVLLMW+LRLLY+ GVSPERLV  YYP
Sbjct:    1 MNKLSIILPVLNEAALIGVALQALQAIRQRGGELVVVDGGSSDDTVQLATALADRVVVAGAGRAQQMNRGAELASGEMLLFLHLDSELPEGAMEFIEQLCGSTANHWGWFDVRLSHPGFAYRVIARFMNLRARLSRVCTGDQALFLSRPLFEQVGGFPELPLMEDVAMSKRLRQLSTPVVCKQPIIASTRRWEERGMLRTVLLMWKLRLLYFFGVSPERLVTLYYP 226
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BLAST of mRNA_D-mesarthrocarpus_Contig10624.3.1 vs. uniprot
Match: A0A3C0P4Q0_9GAMM (Glycosyl transferase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A3C0P4Q0_9GAMM)

HSP 1 Score: 260 bits (664), Expect = 9.460e-85
Identity = 132/226 (58.41%), Postives = 165/226 (73.01%), Query Frame = 0 
Query:    1 MTHFSLIVPVLDEAAQIQQRLEPLQDLRQCGHEVIVVDGGSVDGSLPLAQSLADRILTVARGRARQMNAGARVASNEWLLFLHLDTELPESAAQIMANDIAVSGREWGWFDVQLANPGWPYRVIAWHMNRRARLTRVCTGDQALFVRRCCFEALGGFPDLPLMEDVALSKRLRRRSAPLVLSAPVTTSARRWEQHGVLRTVLLMWRLRLLYWLGVSPERLVNHYYP 226
            M   S+I+PVL+EAA I   L  LQ +R  G E++VVDGGS D ++ LA +LADR++    GRA+QMN GA +AS + LLFLHLD+ELPE A   +      S   WGWFDV+L++PG+ YRVIA  MN RARL+RVCTGDQA+F+ R  FE +GGF +LPLMEDVA+SKRLR+   P+V    V  SARRWE+ GVL TVLLMW+LRLLY+LGVSP++L   YYP
Sbjct:    1 MNKLSIILPVLNEAALIGTALTALQHVRLRGAELLVVDGGSSDDTVQLAAALADRVVVARSGRAQQMNRGAEMASGDILLFLHLDSELPEGAMDFIEQHCGSSANHWGWFDVRLSHPGYAYRVIARFMNLRARLSRVCTGDQAMFLSRPLFEQVGGFSELPLMEDVAMSKRLRKLGPPVVCEHRVIASARRWEEKGVLSTVLLMWKLRLLYFLGVSPDKLAALYYP 226
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BLAST of mRNA_D-mesarthrocarpus_Contig10624.3.1 vs. uniprot
Match: A0A1V0B643_9PSED (Glyco_trans_2-like domain-containing protein n=4 Tax=Pseudomonas TaxID=286 RepID=A0A1V0B643_9PSED)

HSP 1 Score: 257 bits (656), Expect = 4.040e-83
Identity = 134/223 (60.09%), Postives = 162/223 (72.65%), Query Frame = 0 
Query:    5 SLIVPVLDEAAQIQQRLEPLQDLRQCGH-EVIVVDGGSVDGSLPLAQSLADRILTVARGRARQMNAGARVASNEWLLFLHLDTELPESAAQIMANDIAVS--GREWGWFDVQLANPGWPYRVIAWHMNRRARLTRVCTGDQALFVRRCCFEALGGFPDLPLMEDVALSKRLRRRSAPLVLSAPVTTSARRWEQHGVLRTVLLMWRLRLLYWLGVSPERLVNHY 224
            S+I+PV DEAA I Q L PLQ+LR  G  E+IVVDG S D +  LA+ LAD+++    GRARQMNAGA +A  EWLLFLH DT LPE   Q++  D   +     WG FDV+L       R++AW MNRR+RLT VCTGDQA+FV R  FE LGG+ ++PLMED+ LSKRLRRRS PL +S P+TTS+RRW+QHG L T+LLMWRLRLLYWLGV+PERL   Y
Sbjct:   27 SVILPVRDEAAGIVQALLPLQELR--GELEIIVVDGNSNDATRVLAEPLADQVIVTETGRARQMNAGALLARGEWLLFLHADTRLPEGFMQLLPLDAEANTTNHTWGRFDVRLQPSSPLLRLVAWMMNRRSRLTGVCTGDQAIFVHRTLFEQLGGYAEIPLMEDIELSKRLRRRSPPLCISTPLTTSSRRWQQHGTLPTILLMWRLRLLYWLGVAPERLAQLY 247
[Close]
BLAST of mRNA_D-mesarthrocarpus_Contig10624.3.1 vs. uniprot
Match: A0A5C9AYT4_9BACT (Family 2 glycosyl transferase n=1 Tax=bacterium TaxID=1869227 RepID=A0A5C9AYT4_9BACT)

HSP 1 Score: 250 bits (639), Expect = 5.180e-81
Identity = 130/224 (58.04%), Postives = 154/224 (68.75%), Query Frame = 0 
Query:    3 HFSLIVPVLDEAAQIQQRLEPLQDLRQCGHEVIVVDGGSVDGSLPLAQSLADRILTVARGRARQMNAGARVASNEWLLFLHLDTELPESAAQIMANDIAVSGREWGWFDVQLANPGWPYRVIAWHMNRRARLTRVCTGDQALFVRRCCFEALGGFPDLPLMEDVALSKRLRRRSAPLVLSAPVTTSARRWEQHGVLRTVLLMWRLRLLYWLGVSPERLVNHYYP 226
            H S+I+P LDEA  I   L PLQ LR+ GHEVIV DGGS DG+  LA  LADR++   RGRARQMNAGA  A  + LLFLH DT LPE AA+ +A  +A     WG FDV++        V+A  MN R+RLT + TGDQA+FV R  F+A+GGFPD PLMED+ LSKRL+R   P  L+A VTTS RRWE+HGV RT+LLMWRLR  YW GV  +RL   YYP
Sbjct:    2 HLSIILPTLDEADGIDAALAPLQALRRAGHEVIVADGGSTDGTPALATPLADRVIQSTRGRARQMNAGAAAAGGDALLFLHADTRLPEGAAERIAEVLA--DHAWGRFDVRIEGQPALLGVVAAMMNLRSRLTGIATGDQAIFVSRAAFDAVGGFPDQPLMEDIELSKRLKRLDPPACLTARVTTSGRRWERHGVWRTILLMWRLRFDYWRGVPAQRLARRYYP 223
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BLAST of mRNA_D-mesarthrocarpus_Contig10624.3.1 vs. uniprot
Match: A0A7C5NJ55_9GAMM (Glycosyltransferase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A7C5NJ55_9GAMM)

HSP 1 Score: 248 bits (634), Expect = 3.750e-80
Identity = 130/229 (56.77%), Postives = 161/229 (70.31%), Query Frame = 0 
Query:    1 MTHFSLIVPVLDEAAQIQQRLEPLQDLRQCGHEVIVVDGGSVDGSLPLAQSLADRILTVARGRARQMNAGARVASNEWLLFLHLDTELPESAAQIMANDIAVSGREWGWFDVQLANPGWPYRVIAWHMNRRARLTRVCTGDQALFVRRCCFEALGGFPDLPLMEDVALSKRLRRRSAPLVLSAPVTTSARRWEQHGVLRTVLLMWRLRLLYWLGVSPERLVNHYYPRRP 229
            M+  S+IVPVL+EA  I+  L  LQ LRQ GHEVIVVDGGS DG+  +A +LAD +L    GRARQMNAGA  A  + LLFLH DT LP  A +++   +  SGR+WG FDV+LA      R++   MN R+RL+ + TGDQA+FVRR  F A+GGFPD+ LMED+ALS+RL+  S PL L A V TS+RRWEQHGVLRT++LMWRLRL Y+LG  P  L   YY  +P
Sbjct:    1 MSRISVIVPVLNEAPAIRGALSSLQPLRQAGHEVIVVDGGSADGTPDIAAALADSVLRAPPGRARQMNAGAHRARGDILLFLHGDTLLPRQADRLVMEGLRRSGRQWGRFDVRLAGRRPLLRLVETLMNLRSRLSGIATGDQAMFVRREAFTAVGGFPDIALMEDIALSRRLKSLSRPLCLRARVVTSSRRWEQHGVLRTIVLMWRLRLAYFLGADPAALARRYYGDQP 229
[Close]
BLAST of mRNA_D-mesarthrocarpus_Contig10624.3.1 vs. uniprot
Match: A0A4Q5L697_9GAMM (Glycosyltransferase n=2 Tax=Methylococcaceae TaxID=403 RepID=A0A4Q5L697_9GAMM)

HSP 1 Score: 248 bits (633), Expect = 4.660e-80
Identity = 129/220 (58.64%), Postives = 154/220 (70.00%), Query Frame = 0 
Query:    5 SLIVPVLDEAAQIQQRLEPLQDLRQCGHEVIVVDGGSVDGSLPLAQSLADRILTVARGRARQMNAGARVASNEWLLFLHLDTELPESAAQIMANDIAVSGREWGWFDVQLANPGWPYRVIAWHMNRRARLTRVCTGDQALFVRRCCFEALGGFPDLPLMEDVALSKRLRRRSAPLVLSAPVTTSARRWEQHGVLRTVLLMWRLRLLYWLGVSPERLVNHY 224
            S+I+P L+EA +I   L  L  LR  GHE+IVVDGGS DG+   A+S ADR+ +  RGRA QMNAGA +A    LLFLH DTEL  +AA+ + + +  SGR+WG FDV +A      RVIAW MN R+RL+ + TGDQALFVRR  F A+G FPD PLMED+ LSKRLRR S PL L   +TTS RRWE HGV RT+ LMW LR LYW GVSPERL + Y
Sbjct:    6 SIIIPTLNEADRIDSLLASLAPLRAQGHELIVVDGGSADGTAERARSGADRVCSAPRGRALQMNAGAAIAGGSVLLFLHADTELRPTAAEAVLDGLGRSGRDWGRFDVDIAGRHPMLRVIAWCMNHRSRLSGIATGDQALFVRRATFWAVGAFPDQPLMEDIELSKRLRRVSRPLCLRTRITTSGRRWEAHGVWRTIGLMWLLRWLYWRGVSPERLASRY 225
[Close]
BLAST of mRNA_D-mesarthrocarpus_Contig10624.3.1 vs. uniprot
Match: A0A7C1XK95_9GAMM (Glycosyltransferase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A7C1XK95_9GAMM)

HSP 1 Score: 244 bits (624), Expect = 1.160e-78
Identity = 131/226 (57.96%), Postives = 158/226 (69.91%), Query Frame = 0 
Query:    5 SLIVPVLDEAAQIQQRLEPLQDLRQCGHEVIVVDGGSVDGSLPLAQSLADRILTVARGRARQMNAGARVASNEWLLFLHLDTELPESAAQIMANDIAVSGRE--WGWFDVQLANPGWPYRVIAWHMNRRARLTRVCTGDQALFVRRCCFEALGGFPDLPLMEDVALSKRLRRRSAPLVLSAPVTTSARRWEQHGVLRTVLLMWRLRLLYWLGVSPERLVNHYYPRR 228
            S+I+PVLDE   +   L  LQDLR  GHE+++VDGGS D SL LAQ   DRIL    GRARQMNAGA  A+ E LLFLH DT LP +A     NDIA + R   WG FDV+L+   WP R+I   MN R+ LT + TGDQA+FVRR  FE +GG+PD+PLMED+ LS+RL+R S P  + APV TS+RRWE+ G+LRTVLLMW LRL ++LGV P RL   YYP R
Sbjct:    4 SIIMPVLDEGDALADSLAALQDLRAAGHELVLVDGGSRDDSLRLAQGRVDRILHSPPGRARQMNAGAAAATGEVLLFLHADTRLPATAL----NDIAQALRTSVWGRFDVRLSGRAWPLRIIERLMNWRSCLTGMATGDQAIFVRRAVFEQVGGYPDIPLMEDLELSRRLKRLSRPACVRAPVITSSRRWEEQGILRTVLLMWSLRLAWFLGVPPGRLARIYYPGR 225
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BLAST of mRNA_D-mesarthrocarpus_Contig10624.3.1 vs. uniprot
Match: A0A2E9ZI19_9GAMM (Glycosyl transferase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A2E9ZI19_9GAMM)

HSP 1 Score: 244 bits (624), Expect = 1.200e-78
Identity = 125/226 (55.31%), Postives = 159/226 (70.35%), Query Frame = 0 
Query:    5 SLIVPVLDEAAQIQQRLEPLQDLRQCGHEVIVVDGGSVDGSLPLAQSLADRILTVARGRARQMNAGARVASNEWLLFLHLDTELPESAAQIMANDIAVSG--REWGWFDVQLANPGWPYRVIAWHMNRRARLTRVCTGDQALFVRRCCFEALGGFPDLPLMEDVALSKRLRRRSAPLVLSAPVTTSARRWEQHGVLRTVLLMWRLRLLYWLGVSPERLVNHYYPRR 228
            S I+PVL+EAA I+ +L  LQ  R  GHE+++VDGGS D ++  A  LADRI   A GR+ QMN GA +A  + LLFLH+DT LPE A Q+++ ++      R+WGWFD++L+NPG  YR+IA  MN RAR + VCTGDQALFV    F  LGGFP +PLMEDVA+ K LRR + P  +      S+RRW+QHG+++TVLLMW LRL Y+LGVSP  LV  YYPRR
Sbjct:    4 SFILPVLNEAALIRAQLRGLQSYRVEGHEIVLVDGGSSDDTVENAAGLADRIEISAPGRSAQMNRGAELARGDLLLFLHIDTSLPEQADQLISAEMNAGNQRRQWGWFDIRLSNPGLAYRIIAAAMNLRARWSAVCTGDQALFVEVELFHLLGGFPAIPLMEDVAMCKLLRRAARPAPIKTAALASSRRWQQHGLVKTVLLMWWLRLQYFLGVSPRVLVKRYYPRR 229
[Close]
BLAST of mRNA_D-mesarthrocarpus_Contig10624.3.1 vs. uniprot
Match: A0A7V9AJU8_9BACT (TIGR04283 family arsenosugar biosynthesis glycosyltransferase n=1 Tax=Gemmatimonadetes bacterium TaxID=2026742 RepID=A0A7V9AJU8_9BACT)

HSP 1 Score: 244 bits (623), Expect = 1.700e-78
Identity = 129/222 (58.11%), Postives = 156/222 (70.27%), Query Frame = 0 
Query:    3 HFSLIVPVLDEAAQIQQRLEPLQDLRQCGHEVIVVDGGSVDGSLPLAQSLADRILTVARGRARQMNAGARVASNEWLLFLHLDTELPESAAQIMANDIAVSGREWGWFDVQLANPGWPYRVIAWHMNRRARLTRVCTGDQALFVRRCCFEALGGFPDLPLMEDVALSKRLRRRSAPLVLSAPVTTSARRWEQHGVLRTVLLMWRLRLLYWLGVSPERLVNHY 224
            H S+IVP L+EAA I+  LEPLQ LR  GHEVIVVDGGS D ++ LAQ LADR+L   RGRARQMNAGA+ A  E LLFLH DT LP  A + +  ++A S R WG FD++L       R+ A  +  R+RLT + TGDQA+FVRR  FE  GGFP++PLMED+ALS+ L+RR  PL L   VTTS+RRWE+ G+LRTVLLMWRLRL Y+ G  P  L   Y
Sbjct:    7 HLSIIVPALNEAAGIRATLEPLQPLRARGHEVIVVDGGSEDATVALAQPLADRVLVAGRGRARQMNAGAQEARGEVLLFLHADTLLPPEADRRVLEELARSSRGWGRFDLRLTGRHPLLRLTARLIGLRSRLTGMATGDQAIFVRRDWFEQAGGFPEIPLMEDLALSRALKRRGRPLCLHERVTTSSRRWEERGILRTVLLMWRLRLAYYRGADPHDLARRY 228
[Close]
BLAST of mRNA_D-mesarthrocarpus_Contig10624.3.1 vs. uniprot
Match: A0A7C5NF57_9GAMM (Glycosyltransferase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A7C5NF57_9GAMM)

HSP 1 Score: 243 bits (620), Expect = 4.100e-78
Identity = 123/221 (55.66%), Postives = 159/221 (71.95%), Query Frame = 0 
Query:    5 SLIVPVLDEAAQIQQRLEPLQDLRQCGHEVIVVDGGSVDGSLPLAQSLADRILTVARGRARQMNAGARVASNEWLLFLHLDTELPESAAQIMANDIAVSGREWGWFDVQLANPGWPYRVIAWHMNRRARLTRVCTGDQALFVRRCCFEALGGFPDLPLMEDVALSKRLRRRSAPLVLSAPVTTSARRWEQHGVLRTVLLMWRLRLLYWLGVSPERLVNHYY 225
            S+IVPVL+EA+ I   L+ LQ  R  GHEVIVVDGGS D +L LA+ L DR+L    GRARQMN GA+ A  E LLFLH DT LPE+A +++ + +  + R WG FDV+L+     +R+I + MN R+RLTR+ TGDQA+FVR+  F A+GGF D+PLMED+ALS+RL++ +    L   V TS+RRWE++G+ +TVLLMWRLRL YWLG  P RLV  YY
Sbjct:    4 SIIVPVLNEASTIAVCLDSLQPYRAQGHEVIVVDGGSRDDTLALARPLTDRVLVAPAGRARQMNQGAQAARGEVLLFLHADTRLPEAACEVIVSALRTAAR-WGRFDVRLSGAAPAFRMIEFMMNLRSRLTRIATGDQAMFVRKDLFHAVGGFEDIPLMEDIALSRRLKKMAPAACLRQRVVTSSRRWEENGIFQTVLLMWRLRLRYWLGADPARLVRQYY 223
The following BLAST results are available for this feature:
BLAST of mRNA_D-mesarthrocarpus_Contig10624.3.1 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2E9JG77_9GAMM3.930e-9160.62Glycosyl transferase n=1 Tax=Gammaproteobacteria b... [more]
A0A3C0P4Q0_9GAMM9.460e-8558.41Glycosyl transferase n=1 Tax=Gammaproteobacteria b... [more]
A0A1V0B643_9PSED4.040e-8360.09Glyco_trans_2-like domain-containing protein n=4 T... [more]
A0A5C9AYT4_9BACT5.180e-8158.04Family 2 glycosyl transferase n=1 Tax=bacterium Ta... [more]
A0A7C5NJ55_9GAMM3.750e-8056.77Glycosyltransferase n=1 Tax=Gammaproteobacteria ba... [more]
A0A4Q5L697_9GAMM4.660e-8058.64Glycosyltransferase n=2 Tax=Methylococcaceae TaxID... [more]
A0A7C1XK95_9GAMM1.160e-7857.96Glycosyltransferase n=1 Tax=Gammaproteobacteria ba... [more]
A0A2E9ZI19_9GAMM1.200e-7855.31Glycosyl transferase n=1 Tax=Gammaproteobacteria b... [more]
A0A7V9AJU8_9BACT1.700e-7858.11TIGR04283 family arsenosugar biosynthesis glycosyl... [more]
A0A7C5NF57_9GAMM4.100e-7855.66Glycosyltransferase n=1 Tax=Gammaproteobacteria ba... [more]

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InterPro
Analysis Name: InterProScan on OGS1.0
Date Performed: 2022-09-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029044Nucleotide-diphospho-sugar transferasesGENE3D3.90.550.10Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain Acoord: 1..196
e-value: 2.7E-19
score: 71.8
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILY53448Nucleotide-diphospho-sugar transferasescoord: 3..206
IPR026461Transferase 2, rSAM/selenodomain-associatedTIGRFAMTIGR04283TIGR04283coord: 5..224
e-value: 1.1E-80
score: 268.4
IPR026461Transferase 2, rSAM/selenodomain-associatedCDDcd02522GT_2_like_acoord: 5..224
e-value: 1.16768E-91
score: 266.744
IPR001173Glycosyltransferase 2-likePFAMPF00535Glycos_transf_2coord: 5..115
e-value: 7.2E-11
score: 42.2
NoneNo IPR availablePANTHERPTHR43646GLYCOSYLTRANSFERASEcoord: 3..227

Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
D-mesarthrocarpus_Contig10624contigD-mesarthrocarpus_Contig10624:1789..2478 +
Analyses
This polypeptide is derived from or has results from the following analyses
Analysis NameDate Performed
InterProScan on OGS1.02022-09-29
Diamond blastp: OGS1.0 vs UniRef902022-09-16
Discosporangium mesarthrocarpum MT17_79 OGS1.02022-07-08
Relationships

This polypeptide derives from the following mRNA feature(s):

Feature NameUnique NameSpeciesTypePosition
mRNA_D-mesarthrocarpus_Contig10624.3.1mRNA_D-mesarthrocarpus_Contig10624.3.1Discosporangium mesarthrocarpum MT17_79mRNAD-mesarthrocarpus_Contig10624 1789..3119 +


Sequences
The following sequences are available for this feature:

polypeptide sequence

>prot_D-mesarthrocarpus_Contig10624.3.1 ID=prot_D-mesarthrocarpus_Contig10624.3.1|Name=mRNA_D-mesarthrocarpus_Contig10624.3.1|organism=Discosporangium mesarthrocarpum MT17_79|type=polypeptide|length=230bp
MTHFSLIVPVLDEAAQIQQRLEPLQDLRQCGHEVIVVDGGSVDGSLPLAQ
SLADRILTVARGRARQMNAGARVASNEWLLFLHLDTELPESAAQIMANDI
AVSGREWGWFDVQLANPGWPYRVIAWHMNRRARLTRVCTGDQALFVRRCC
FEALGGFPDLPLMEDVALSKRLRRRSAPLVLSAPVTTSARRWEQHGVLRT
VLLMWRLRLLYWLGVSPERLVNHYYPRRP*
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Annotated Terms
The following terms have been associated with this polypeptide:
Vocabulary: INTERPRO
TermDefinition
IPR029044Nucleotide-diphossugar_trans
IPR026461Trfase_2_rSAM/seldom_assoc
IPR001173Glyco_trans_2-like