mRNA_D-mesarthrocarpus_Contig912.5.1 (mRNA) Discosporangium mesarthrocarpum MT17_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_D-mesarthrocarpus_Contig912.5.1
Unique NamemRNA_D-mesarthrocarpus_Contig912.5.1
TypemRNA
OrganismDiscosporangium mesarthrocarpum MT17_79 (Discosporangium mesarthrocarpum MT17_79)
Homology
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BLAST of mRNA_D-mesarthrocarpus_Contig912.5.1 vs. uniprot
Match: D8LKA1_ECTSI (eIF-2B GDP-GTP exchange factor subunit epsilon n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LKA1_ECTSI)

HSP 1 Score: 171 bits (432), Expect = 6.280e-46
Identity = 86/127 (67.72%), Postives = 102/127 (80.31%), Query Frame = 2 
Query:    2 ECMRPAMEVVLDLAGAGGTGAQGKALIPAVQRQMVHWKPLLSNLKVEPGDDLEMIKVVEGYALRPECEKSLRAVFRYVLQLLLNEEMVDEECLMRWVTLRRSTPGETPERKLFDEREVQDFVEWLEE 382
            ECMRPA+E+VLDLAGA  TG  G AL+ AV+ Q+ HWKPLLS L VE GDD+EMIKVVE YALRPECE +LRAVFRYV+Q    EEMV E+CL RW+ LRR+TP  +PER LF+++EVQ FV WLE+
Sbjct:  508 ECMRPAVELVLDLAGAD-TG-NGMALVAAVKTQLRHWKPLLSKLNVEAGDDVEMIKVVESYALRPECEGTLRAVFRYVVQSFFQEEMVSEDCLERWIQLRRATPQGSPERGLFEQKEVQAFVAWLED 632
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BLAST of mRNA_D-mesarthrocarpus_Contig912.5.1 vs. uniprot
Match: A0A835YX04_9STRA (eIF-2B GDP-GTP exchange factor subunit epsilon n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YX04_9STRA)

HSP 1 Score: 114 bits (285), Expect = 1.480e-25
Identity = 60/128 (46.88%), Postives = 79/128 (61.72%), Query Frame = 2 
Query:    2 ECMRPAMEVVLDLAGAGGT-GAQGKALIPAVQRQMVHWKPLLSNLKVEPGDDLEMIKVVEGYALRPECEKSLRAVFRYVLQLLLNEEMVDEECLMRWVTLRRSTPGETPERKLFDEREVQDFVEWLEE 382
            EC+R + E VLDLAG G    A G  L+ AV+ Q+  WK LLS L     D +E+I  VE YALRP     L  VFR++LQLL + E+V EE + +W+ LRRS P   PE  LF+  + Q+F+ WLE+
Sbjct:  602 ECIRASTEAVLDLAGCGKPDAAAGMPLVAAVREQLQLWKTLLSRLAQNAADQVEVIMAVEQYALRPGKATPLAGVFRFILQLLYDTEVVSEEGMEQWIALRRSGPATAPEMTLFNAPQTQEFIAWLED 729
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BLAST of mRNA_D-mesarthrocarpus_Contig912.5.1 vs. uniprot
Match: A0A7S2XW38_9STRA (Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XW38_9STRA)

HSP 1 Score: 85.5 bits (210), Expect = 2.120e-17
Identity = 44/125 (35.20%), Postives = 76/125 (60.80%), Query Frame = 2 
Query:    5 CMRPAMEVVLDLAGAGGTGAQGKALIPAVQRQMVHWKPLLSNLKVEPGDDLEMIKVVEGYALRPECEKSLRAVFRYVLQLLLNEEMVDEECLMRWVTLRRSTPGETPERKLFDEREVQDFVEWLE 379
            C++  +  +LDL   G +     AL   ++  +  WK L+++L ++  D+LE+IK  E + L  E +++L  +FR VLQ++ + ++V E+ L+ W+ LRR+  GE  E+ LFD  + Q FVEW+E
Sbjct:   32 CLQGVVPGILDLVEIGSSDQM--ALASKMKLTLTTWKSLITDLSMDENDELEVIKTTEFWVLSSEHKETLTPLFRLVLQIMYDLDVVSEDALLAWIDLRRNEDGE--EKVLFDHPQTQAFVEWIE 152
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BLAST of mRNA_D-mesarthrocarpus_Contig912.5.1 vs. uniprot
Match: A0A7S3XLN7_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XLN7_HETAK)

HSP 1 Score: 68.6 bits (166), Expect = 6.450e-11
Identity = 42/125 (33.60%), Postives = 64/125 (51.20%), Query Frame = 2 
Query:   50 GGTGAQGKA---LIPAVQRQMVHWKPLLSNLKVEPGDDLEMIKVVEGYALRPECEKSLRAVFRYVLQLLLNEEMVDEECLMRWVTLRRS------TPGETPERK-----LFDEREVQDFVEWLEE 382
            G T   GK    ++   +  +  W  L+S L VE  D + +IK +E Y ++   + +L  +FR +LQLL + E++ E+ L+ W  LRRS        G + ER      LF   + Q+FV WLEE
Sbjct:   14 GATDPAGKTAMQIVTGYKNTLATWGELISKLCVEDKDQMAVIKAIEKYVVQSAEKATLIPLFRLILQLLYDAEVLAEDALLEWADLRRSGDQDEDEEGASAERHAEVLALFQHPQTQEFVTWLEE 138
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BLAST of mRNA_D-mesarthrocarpus_Contig912.5.1 vs. uniprot
Match: B8CFK7_THAPS (eIF-2B GDP-GTP exchange factor subunit epsilon n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8CFK7_THAPS)

HSP 1 Score: 61.6 bits (148), Expect = 2.060e-7
Identity = 34/130 (26.15%), Postives = 64/130 (49.23%), Query Frame = 2 
Query:    2 ECMRPAMEVVLDLAGAGGTGAQGKALIPAVQRQMVHWKPLLSNLKVEPGDDLEMIKVVEGYAL---RPECEKSLRAVFRYVLQLLLNEEMVDEECLMRWVTLRRSTPGETPERKLFDEREVQDFVEWLEE 382
            +C + AM  V+D             L+ +++  + +W  L  ++ +   ++  +I  +E  AL   +     +    FR+VLQ L ++E+V+E+ +  W   R+     +P   LF ++  QDF+EWLEE
Sbjct:  765 DCCKGAMMAVMDKVLEKSDNPTPGKLVASLRTMLSYWGSLFQSICIGSEEEKAIIHALETMALGSGKGSVALATEPAFRFVLQTLHDQEIVNEDAIFEWAAERKEEEKGSPRGALFWQKPTQDFLEWLEE 894
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BLAST of mRNA_D-mesarthrocarpus_Contig912.5.1 vs. uniprot
Match: M4B7H9_HYAAE (W2 domain-containing protein n=1 Tax=Hyaloperonospora arabidopsidis (strain Emoy2) TaxID=559515 RepID=M4B7H9_HYAAE)

HSP 1 Score: 54.3 bits (129), Expect = 5.680e-5
Identity = 26/111 (23.42%), Postives = 56/111 (50.45%), Query Frame = 2 
Query:   53 GTGAQGKALIPAVQRQMVHWKPLLSNLKVEPGDDLEMIKVVEGYALRPECEKSL-RAVFRYVLQLLLNEEMVDEECLMRWVTLRRSTPGETPERKLFDEREVQDFVEWLEE 382
            G      A++  V+ +   W  ++    VE  D L ++  +E Y   PE  ++L   +FR++LQ + + E V E+ ++ W    +++           +++VQ+F++WL++
Sbjct:  566 GQELSAMAILGHVRTKFQKWSSVIQRCIVEQEDQLAVVNALESYCAAPEERRTLWLPLFRFLLQTVYDLEWVGEDVILEWHKTTQASEAPVDALTAASKKDVQEFIDWLQQ 676
The following BLAST results are available for this feature:
BLAST of mRNA_D-mesarthrocarpus_Contig912.5.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
D8LKA1_ECTSI6.280e-4667.72eIF-2B GDP-GTP exchange factor subunit epsilon n=2... [more]
A0A835YX04_9STRA1.480e-2546.88eIF-2B GDP-GTP exchange factor subunit epsilon n=1... [more]
A0A7S2XW38_9STRA2.120e-1735.20Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa... [more]
A0A7S3XLN7_HETAK6.450e-1133.60Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
B8CFK7_THAPS2.060e-726.15eIF-2B GDP-GTP exchange factor subunit epsilon n=1... [more]
M4B7H9_HYAAE5.680e-523.42W2 domain-containing protein n=1 Tax=Hyaloperonosp... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
D-mesarthrocarpus_Contig912contigD-mesarthrocarpus_Contig912:18768..21214 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Discosporangium mesarthrocarpum MT17_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D8LKA1
Preferred nameEIF2B5
PFAMsHexapep,NTP_transferase,W2
Model size678
Max annot lvl2759|Eukaryota
KEGG koko:K03240,ko:K03859
KEGG ReactionR05916
KEGG Pathwayko00563,ko01100,ko03013,map00563,map01100,map03013
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0001541,GO:0002181,GO:0002183,GO:0002253,GO:0002376,GO:0002429,GO:0002682,GO:0002684,GO:0002757,GO:0002764,GO:0002768,GO:0003006,GO:0003674,GO:0003676,GO:0003723,GO:0003743,GO:0005085,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005829,GO:0005851,GO:0006412,GO:0006413,GO:0006417,GO:0006446,GO:0006518,GO:0006807,GO:0006950,GO:0007154,GO:0007165,GO:0007166,GO:0007272,GO:0007275,GO:0007399,GO:0007417,GO:0007420,GO:0007548,GO:0007568,GO:0008135,GO:0008150,GO:0008152,GO:0008366,GO:0008406,GO:0008585,GO:0009058,GO:0009059,GO:0009266,GO:0009408,GO:0009628,GO:0009719,GO:0009725,GO:0009743,GO:0009746,GO:0009749,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0010001,GO:0010033,GO:0010035,GO:0010038,GO:0010226,GO:0010243,GO:0010467,GO:0010468,GO:0010556,GO:0010557,GO:0010604,GO:0010608,GO:0010628,GO:0010941,GO:0010942,GO:0014002,GO:0014003,GO:0019222,GO:0019538,GO:0019899,GO:0021537,GO:0021543,GO:0021761,GO:0021766,GO:0021782,GO:0022008,GO:0022414,GO:0023052,GO:0030154,GO:0030900,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031369,GO:0032045,GO:0032268,GO:0032270,GO:0032501,GO:0032502,GO:0032991,GO:0033554,GO:0034248,GO:0034250,GO:0034284,GO:0034641,GO:0034645,GO:0034976,GO:0042063,GO:0042221,GO:0042552,GO:0042981,GO:0043043,GO:0043065,GO:0043067,GO:0043068,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043434,GO:0043603,GO:0043604,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044424,GO:0044444,GO:0044464,GO:0045137,GO:0045727,GO:0045948,GO:0046545,GO:0046660,GO:0048468,GO:0048513,GO:0048518,GO:0048522,GO:0048583,GO:0048584,GO:0048608,GO:0048708,GO:0048709,GO:0048731,GO:0048856,GO:0048869,GO:0050776,GO:0050778,GO:0050789,GO:0050794,GO:0050851,GO:0050852,GO:0050896,GO:0051020,GO:0051171,GO:0051173,GO:0051246,GO:0051247,GO:0051716,GO:0060255,GO:0060322,GO:0061458,GO:0065007,GO:0065009,GO:0071704,GO:0080090,GO:0097159,GO:0098772,GO:1901363,GO:1901564,GO:1901566,GO:1901576,GO:1901652,GO:1901698,GO:1901700,GO:2000112
Exons4
Evalue6.64e-46
EggNOG OGsCOG1208@1|root,KOG1461@2759|Eukaryota
Ec32 ortholog descriptioneukaryotic translation initiation factor 2B epsilon subunit
Ec32 orthologEc-01_009500.3
Descriptiontranslation initiation factor activity
Cds size450
COG categoryJM
BRITEko00000,ko00001,ko03012
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_D-mesarthrocarpus_Contig912.5.1prot_D-mesarthrocarpus_Contig912.5.1Discosporangium mesarthrocarpum MT17_79polypeptideD-mesarthrocarpus_Contig912 18989..21207 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680791067.3582087-UTR-D-mesarthrocarpus_Contig912:18767..189881680791067.3582087-UTR-D-mesarthrocarpus_Contig912:18767..18988Discosporangium mesarthrocarpum MT17_79UTRD-mesarthrocarpus_Contig912 18768..18988 -
1680791067.421081-UTR-D-mesarthrocarpus_Contig912:21207..212141680791067.421081-UTR-D-mesarthrocarpus_Contig912:21207..21214Discosporangium mesarthrocarpum MT17_79UTRD-mesarthrocarpus_Contig912 21208..21214 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680791067.3731463-CDS-D-mesarthrocarpus_Contig912:18988..191421680791067.3731463-CDS-D-mesarthrocarpus_Contig912:18988..19142Discosporangium mesarthrocarpum MT17_79CDSD-mesarthrocarpus_Contig912 18989..19142 -
1680791067.3836255-CDS-D-mesarthrocarpus_Contig912:19672..197831680791067.3836255-CDS-D-mesarthrocarpus_Contig912:19672..19783Discosporangium mesarthrocarpum MT17_79CDSD-mesarthrocarpus_Contig912 19673..19783 -
1680791067.3935826-CDS-D-mesarthrocarpus_Contig912:20241..203661680791067.3935826-CDS-D-mesarthrocarpus_Contig912:20241..20366Discosporangium mesarthrocarpum MT17_79CDSD-mesarthrocarpus_Contig912 20242..20366 -
1680791067.4070807-CDS-D-mesarthrocarpus_Contig912:21147..212071680791067.4070807-CDS-D-mesarthrocarpus_Contig912:21147..21207Discosporangium mesarthrocarpum MT17_79CDSD-mesarthrocarpus_Contig912 21148..21207 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_D-mesarthrocarpus_Contig912.5.1

>prot_D-mesarthrocarpus_Contig912.5.1 ID=prot_D-mesarthrocarpus_Contig912.5.1|Name=mRNA_D-mesarthrocarpus_Contig912.5.1|organism=Discosporangium mesarthrocarpum MT17_79|type=polypeptide|length=150bp
MRPAMEVVLDLAGAGGTGAQGKALIPAVQRQMVHWKPLLSNLKVEPGDDL
EMIKVVEGYALRPECEKSLRAVFRYVLQLLLNEEMVDEECLMRWVTLRRS
TPGETPERKLFDEREVQDFVEWLEEEEEEDDDEDEDEDDGSSEDFEEEG*
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mRNA from alignment at D-mesarthrocarpus_Contig912:18768..21214-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_D-mesarthrocarpus_Contig912.5.1 ID=mRNA_D-mesarthrocarpus_Contig912.5.1|Name=mRNA_D-mesarthrocarpus_Contig912.5.1|organism=Discosporangium mesarthrocarpum MT17_79|type=mRNA|length=2447bp|location=Sequence derived from alignment at D-mesarthrocarpus_Contig912:18768..21214- (Discosporangium mesarthrocarpum MT17_79)
GGAGTGCATGCGCCCAGCAATGGAAGTGGTGCTGGACCTAGCAGGGGCGG
GGGGGACTGGGGCACAGGTGTGGTGCGAGGGGGGTTGGCCATCGCTATCT
AAGATGAAGATAATGCCGACTGACCATTGATGTTCGACCGCTTCTGTTTC
TGGAACACCTGAGTGTAACGGTTTCTGACGGGACAGGTATTGCTGCCTTT
GGAAATTGCCTTGCGGGTACGTTGTACAGTGGAGCACTACCGTAGCAGAA
AAACGTTTCATGTTCTATAGGAAGAGGTGTATTTTGTTTTGTGGAGAGTA
TGGCTGAGGTTCTCTGGGCTCAACTCATGCTGTACGTATCTCCCGCCAAG
GAGTTTGTACAGGATTCCTTGGCCTCTTTTTTTGCATGGTGTGTTGGTTT
GCTGTCTTCATTTTGTTGGCGGCGCGTTACTATTGCCTCAAAGTATGCAT
GCACTCAATTCTATGGTACTCTTGTGTGTTTTCGAGGTACTGGTGTATAT
GCTGTGATCACCGTGTTGGAGTCATGAAAGTAACTTTGGAGCTTGTTTGA
AGGTTCAAGTTTAAAGTGGTGAGTCCCAAGCCGTGCCAGCAGCCCTACCA
CGGTCGATCACTCTGATCAAACAACGGCAAAAATGTGTAGGAATGTCTCC
TTTCCTGACACAAAAGCACCTGCTGACCTACCGGAGTACCAAGATTATCC
AGACAACTTGAAGCCCAAAACTGGTGTGATGAAATTGCCCCAACTGTGGT
CTCTGGGTGGATATTCATCAAACAATGAAGTAGACAATGTCCTTTGCATT
GTGAAGGGCTGCCAAGTGTGGTAATATGTTCCGTGTGTGTGGTTGCAGGG
CAAGGCTTTGATTCCAGCTGTCCAGCGCCAGATGGTGCACTGGAAGCCCC
TCTTGAGTAACCTCAAGGTTGAGCCGGGAGACGATCTGGAAATGATCAAG
GTCGTTGAGGGGTATGCCCTTAGGTGAGCTTTAGCCCAGGGTTGTGGAGA
ATAGGGGGGAAGGGTGGGTGGAGTCATGCATCACATACTGTGGGATTGAA
ATGTCGTTGGCAGGTCGTTGGATGATGCTACACTGCATGAAGACCTTCTT
GTGCTGGTGCTTGGGAGTAGGCTACAACACAGCTTGTGAATCAACCAGGC
ATTTGTTGCACCATGCTTTAAGCTTACCGTTGTCTGGATGACTCTTGCTC
CTGCTGGTGGCAACACGAACAAAAGAAATACGTAACAAGGTTGCTTAAAT
AGTTGATAGTTTTCCCGCATTGCGGGAGAAAAATCTGCGCATTAGTTTGA
AATTCAGAGTACCCCATGAGTACAAAGCTAAAAGCATATCCCTACCCCTA
CCCCTACCCCTTGGATCAAACTTCGAAGATGAGCTCTACAGTACACTGCC
TCCCTTTGCTCCCCCTTTGTGCTTGGCCTAGGCCTGAATGTGAGAAGAGC
CTTCGCGCTGTGTTCCGGTATGTCCTCCAGCTCCTTCTCAATGAAGAGAT
GGTGGATGAAGAGTGCCTAATGCGCTGGGTCACACTCCGGAGGTGGGCAA
GGGGAATGCTGAGATAGTTCACCTCGCTATCCTTTTGGAGCAGTGAGGTT
TTGGAGGAGGCTCTGTTACAAGTAGGGCAGTGACAGGTGGTGCAGCCAGG
AGGATGCTGCACGCATGCATGTGGTGTGGTGTGGTGTGGTGTGGTGTGGT
GTGGCGTGGTGTATATATCAACCCTGTCTGTGTCCTCAGTTATGCTTATT
CCATGCTCAGCACCGGTCAAAGAAAAACGAAATAATTTGCCCCCCTTGGA
AGCTACTCGATGCCCGCCAAGCAGTTGCTCCAACGGACAGCTACACCATC
ATTTTGTGCACAGTTGCAGCAGCCTTGACTCAACCTTATTCCACCTGAGG
ACCATCGGATGTGTTCATGGAGGTTGTGCTACAATGGCACTTCAGCGCAG
TAGGAATGTGACAAGGGCTTTTCTCATCTCATTCTCACCATCATCAGTCA
CTTACTCTCAATGAATCCAACGGACTATAAATTAAGCTGACGCTTCCCCT
TGTGGCAAAAAAAATGATGAAGGTCCACGCCTGGAGAGACTCCAGAGCGA
AAACTTTTTGATGAGCGGGAAGTGCAAGATTTTGTTGAGTGGCTCGAGGA
GGAGGAGGAGGAAGATGACGATGAGGACGAGGATGAGGATGATGGTTCTT
CTGAGGACTTTGAGGAAGAAGGCTAATCTGAGTGACCCCACGAACGAGAA
GAAAGATATGTCTGGCAGGGGACGGCCTGACTAGTTTTGAAATGACCTTG
GATGGGCACAGTCGACTGATGAAGTGCATGGCGAGGGGGAATATGTTTGA
TTTTCCGTGGAAAGGAAGCTGAAGGAGTGCTTCGCCAAAACCAGGGTTTC
CTCTCGACTTTTTAAAACTTAATTTCCTATCATATCTCTTCATCATG
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Coding sequence (CDS) from alignment at D-mesarthrocarpus_Contig912:18768..21214-

>mRNA_D-mesarthrocarpus_Contig912.5.1 ID=mRNA_D-mesarthrocarpus_Contig912.5.1|Name=mRNA_D-mesarthrocarpus_Contig912.5.1|organism=Discosporangium mesarthrocarpum MT17_79|type=CDS|length=450bp|location=Sequence derived from alignment at D-mesarthrocarpus_Contig912:18768..21214- (Discosporangium mesarthrocarpum MT17_79)
ATGCGCCCAGCAATGGAAGTGGTGCTGGACCTAGCAGGGGCGGGGGGGAC
TGGGGCACAGGGCAAGGCTTTGATTCCAGCTGTCCAGCGCCAGATGGTGC
ACTGGAAGCCCCTCTTGAGTAACCTCAAGGTTGAGCCGGGAGACGATCTG
GAAATGATCAAGGTCGTTGAGGGGTATGCCCTTAGGCCTGAATGTGAGAA
GAGCCTTCGCGCTGTGTTCCGGTATGTCCTCCAGCTCCTTCTCAATGAAG
AGATGGTGGATGAAGAGTGCCTAATGCGCTGGGTCACACTCCGGAGGTCC
ACGCCTGGAGAGACTCCAGAGCGAAAACTTTTTGATGAGCGGGAAGTGCA
AGATTTTGTTGAGTGGCTCGAGGAGGAGGAGGAGGAAGATGACGATGAGG
ACGAGGATGAGGATGATGGTTCTTCTGAGGACTTTGAGGAAGAAGGCTAA
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