prot_D-herbacea_F_contig113.1036.1 (polypeptide) Desmarestia herbacea DmunF female

BLAST of mRNA_D-herbacea_F_contig113.1036.1 vs. uniprot
Match: D7FTQ1_ECTSI (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FTQ1_ECTSI)

HSP 1 Score: 1375 bits (3559), Expect = 0.000e+0
Identity = 777/1422 (54.64%), Postives = 925/1422 (65.05%), Query Frame = 0

Query:    2 PLDGGVPPALFARAMTSLAPHYRDDYDALRAHLQEEHAVFKA-ESPDVAR-----------VHLCCTDYRNGPAVKSALESACGGKSFVRTALN-NVLSGYCAVAHLSSASAAEAHSLVHADVRCSPLTHASKEPESLLAPSSSGNGGG-------------LFGLDLGRSLQDGPGRGLVVTMSPGSLPVE-AASVLAPPNTRGAAGTRGGADSNRKLLEGEKQRGLTAFEGLEERWRTRWGDAQGRRGAREMAEMTPWT------SSSRVSGGGDGRRSLMNGRGEG---------RGSSAQRFHGGKAAGYGAALEHLGEEMAA-------EGGEELPLSEACGWDSNMSFVHARKDVMYLRGIHHADHDEATATACFLALVGFLSAQPEVMHIHPEAIPAPTNKVLTAYVEGGGDSSTPMRTAGLDGSGEVIGITDTGLDDHSCFFRDDENGLVPRTTMSEASVYPDQRKVIQYVVYADDKDENGHGTHVSGIAAGAITDGWKAPWESQFTEAKCEDEGHVRSCLGSCVEEMVGGECHWNQELSCPMSDCDEDTSCDDDTGYTFPCFGDPVDELPEASGMAPASKIAFFDIGTADDGLVIPDNIGDMWDAQYAAGARIMTNSWSLVTVTEPSVRDVELDEWVHDHPDAFVVFGAGNNGYEDGGFNPGSVQSPATAKTAMTVGCSNSGPDRAYAYYTTASSNYEGVMWPEDDVSFEVSPFSARGPVGRFTAKPDVIAPGLMVHSARASRSEDGEETCALDSYGGTSMSAPAIAGAAALVRQYFRQGVFAKYLDDEGLCN-----------SDGIHTEAGLCKAFEPSGYLVKAVLINSAEWLGNMQTVSYDDDAEWMTVLDFAQGFGAVQMASGMPTKDAPVGAFYHENALATDRLHYYSIYVADPSKDVSVTVAWYDPPSTVASYN-----------------------LLHDLDLFVMSVESRKYFRSNSYNTMLSMCADNETCANKYEFTFGNNLADYMNPVERISIPSSDLDEGYYVIGVRARTLTESDSQAYGLAASGGDLVLHDMAKYWSDLGAETQSTDGNG--------DEPNPDADNNEPLAPSPVPPLTSSPTLLDSSGTGNEIVLAPPGTPSPTVQDASRGAEAASXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPTADLSGGGSGGESGWTDNSKIAIVASVASAVVLIGVGICLARRRR----NGGKDETGSFFAGVVSTGSGRSARIVHVG-----------------------DESDGGEEDSL-------EADPRVPLGARELPSYATAVACPPEEEGTTVISDASGSREVIELTAADESEMSGFYAAVDPTAVSKLVGWGIGRDFARVALRQTENDVPEALKLIAEGNMDEQLAMDHDEMAQEGAQAAAA 1298
            P D  VP  LF RAMTSL  HY+DD++AL AHL+EEHA+F A E+PD AR            HLCCTDYR+GPA+KSALE+A GGK FVRTALN N L GYCAVAH+SS++A          VRCSP+THASKEP SLLAPS++                  LFGL LG  +QDG  RGLV+TMSPGSLP++ AA  +A  NTRG  G   GA S R+ L   +Q   ++   LE  WR  WG A+G  GAR +AE  PWT                   SL+ G G G         R S A+ FHGGKAAGYGAAL HLGE+MA        EGG  + L+  CG  +N+ FVH   D++YLRGIHH +HDE T+ ACF+ LVGFLSAQPEVMHIHPE      NKVLTAY+EGG  + TPMR AGL G+G+VIG+TDTGLDD SCFF+D+E G VPRTTM+ A  YPDQRKVIQYVV+ADD D +GHGTHV+GIAAG + D W+  W    ++  C+D G + SC G CV   +GG CHWN ELSCPMSDCDED SCDD T Y FPCF DPVDELPEASG+A  +++AFFDIG  D GLVIPD+IGDMWDAQYAAGAR+++NSWSLVT+TEP+ RDV+LDEWVHDHPD  VVFGAGNNG+ED GFNPGSVQSPAT KTAMTVG SNSGP RAYAYY  A+SN++GV+WP D+  F+VSPFSARGPVGRFTAKPDV+APGLMVHSA+AS S+ GEETCALDS GGTSMSAPA+AGAAALVRQ+F QG  A YLD  GLC            + G++ EAGLC+AF PSGYLVKAVL+NSA WLG+MQ VSY+D  ++M+VLDFAQGFGAVQ+A+ +PT+ AP+GAFYHE+ LA+D LHYY IYV+D SK VSVTVAW+DPPS V+SYN                       LLHDLD+FVMSV++R+Y+RSN ++T+ +MC  +E+CA KYEFT+ N+LADYMNPVER+++PSSDL+EGY+V+GVRARTLTESD QAYG+AA+GG LVLH M+K W+DL  ET ST G G                                        TG+ + +AP   P  T   ++ G+  +                                             D+ GG                         LIG GICLA RR     +G +DETGSF   V +  S                                 D + G E+D +       +  P  P   R LP YATAVA  PEEEG T+I D +G   ++ELTAADESE+SGFY AVDP AV  LV WGI RDFARVALRQT+NDV EAL++IAEGNMDE LAMDH+EMA+EG +AAAA
Sbjct:   64 PADSSVPAGLFIRAMTSLPGHYQDDHEALEAHLREEHAIFAASETPDDARSXXXXXXXXXXAHLCCTDYRDGPALKSALEAATGGKRFVRTALNSNSLGGYCAVAHVSSSAAXXXXXXXAETVRCSPVTHASKEPLSLLAPSTATESXXXXXXXXXXSGRTPLFGLRLGPGVQDGDSRGLVITMSPGSLPMDDAAEDVAKGNTRG--GDEAGAGSMRRNLSTRQQ--SSSLAALERTWRDFWGGARGSDGARALAETVPWTIPPGXXXXXXXXXXXXXXXSLLAGDGTGDASEPSSGVRVSRAREFHGGKAAGYGAALAHLGEKMAKRDDDEEEEGGVGVTLAGVCGLGNNLEFVHGGNDLLYLRGIHHPEHDEETSAACFIGLVGFLSAQPEVMHIHPEPRAEANNKVLTAYIEGGEPTETPMRVAGLTGAGQVIGVTDTGLDDSSCFFKDEE-GSVPRTTMALAEYYPDQRKVIQYVVFADDTDTDGHGTHVAGIAAGKVYDDWEEAWVGDVSKEVCDDAGLIMSCFGDCVAGTMGGSCHWNPELSCPMSDCDEDVSCDDYTQYDFPCFEDPVDELPEASGVASDAQLAFFDIGNEDGGLVIPDDIGDMWDAQYAAGARVISNSWSLVTMTEPTARDVQLDEWVHDHPDTLVVFGAGNNGFEDHGFNPGSVQSPATGKTAMTVGASNSGPGRAYAYYDDAASNFDGVLWPSDEEMFQVSPFSARGPVGRFTAKPDVVAPGLMVHSAKASLSDPGEETCALDSLGGTSMSAPAVAGAAALVRQFFEQGFLASYLDGAGLCGGTDENEEVVAAAGGVYAEAGLCEAFAPSGYLVKAVLVNSAMWLGDMQMVSYEDKTQYMSVLDFAQGFGAVQLAASVPTEGAPMGAFYHEHELASDELHYYLIYVSDASKTVSVTVAWFDPPSPVSSYNRSSSWAQESMMTFMTFLRLRRPKLLHDLDMFVMSVDARRYYRSNHHDTIATMCDADESCAEKYEFTYENDLADYMNPVERVTVPSSDLEEGYHVVGVRARTLTESDVQAYGVAAAGGGLVLHGMSKNWTDLVLETDSTQGTGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTGDSVAIAPTSDPVETATGSTSGSGTSMAGDEDEFSGDSTGSGAGVSHLGGGDDDISAAGGIETDVPEGGDLDVDGGXX------XXXXXXXXXXXXXXXXXLIGAGICLAVRRNKLRSSGRRDETGSFLCPVRNASSSAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDRNHGEEDDRVVGFYDGGDMPPLEPQ--RGLPGYATAVASMPEEEGHTIIRDRAGVERMVELTAADESEVSGFYYAVDPGAVETLVSWGISRDFARVALRQTDNDVQEALQMIAEGNMDELLAMDHEEMAREGREAAAA 1472          

BLAST of mRNA_D-herbacea_F_contig113.1036.1 vs. uniprot
Match: A0A4D9D243_9STRA (Subtilisin n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D243_9STRA)

HSP 1 Score: 262 bits (670), Expect = 4.690e-68
Identity = 214/668 (32.04%), Postives = 317/668 (47.46%), Query Frame = 0

Query:  325 DEATATACFLALVGFLSAQPEVMHIHPEAIPAPTNKVLTAYVEGGGDSS--TPMRTAGLDGSGEVIGITDTGLDDHSCFFRDDENGLVPRTTMSEASVYPDQRKVIQYVVYADDKD-ENGHGTHVSGIAAGAITDGWKAPWESQFTEAKCEDEGHVRSCLGSCV-------EEMVGGECH-WNQE------LSCPMSDCDEDTSCDDDTGYTFPCFGDPVDELPEASGMAPASKIAFFDIGTADDGLVIPDNIGDM-WDAQYAAGARIMTNSWSLVTVTEPSVRDVELDEWVHDHPDAFVVFGAGNNGYEDGGFNPGSVQSPATAKTAMTVGCSNSGPDRAYAYYTTASSNYEGVMWPEDDVSFEVSPFSARGPVGRFTAKPDVIAPGLMVHSARASRSEDGEETCALDSYGGTSMSAPAIAGAAALVRQYFRQGVFAKYLDDEGLCNSDGIHTEAGLCKAFEPSGYLVKAVLINSAEWLGNMQTVSYDDDAEWMTVLDFAQGFGAVQMASGMPTKDAPVGAFYHENALAT--DRLHYYSIYVADPSKDV--SVTVAWYDPPST-VASYNLLHDLDLFVMSVESRKYFRSNSYNTMLSMCADNETCANKYEFTFGNNLADYMNPVERISIPSSDLDEGYYVIGVRARTLTESDSQAYGLAASGGDLVL 969
            DEA   AC   LV F+++ P+++ I   A  A  N   T  ++ G +++   P+   GL G GE+IG+ DTGLDD SCFF D+  G V R++         QRKV+QYV + D  D   GHGTHVSG  AG +      P         C  EG   +C G C+       +E     C  W Q+       +CP+  CD      D  G    C  +P + L    GMA  +KIAFFDI      L  P +   M +   Y AGARI +NSW +  V E     +++D++ ++H D  +++ AGN  Y + GF+  S+ SP   K  +TVG + SGP+R      +   NY             ++ FS+RGP      KPD+++PG  ++SAR++   +G  TC+     GTSM+AP +AGAAAL+RQYF++G +   L   G C       +       +PS  L+KA+ +NSA  +  ++    D+        D AQG G +++ + +         F  + +LA+   R +Y+ I  A  +  V   VT+ W DPP++  A   LLHDLDL V+     +++ +                        G    D  N VE+I +    L  G Y + V A  L+E++ Q Y L A+G    L
Sbjct:   89 DEADRQACLEGLVAFIASHPDILSIQTRATYALQNDQATWIIQSGDEATHARPLWDLGLTGKGELIGVADTGLDDESCFFYDNALGQVARSSWEAPFTDMTQRKVVQYVSFVDGGDLVEGHGTHVSGSLAGVVE-----PRNEGRRGHVCA-EGETLACTGMCLSAPTCQLKEYGYVSCQDWLQDGHCDAWFACPLYGCDGG----DCVGLA--CNPNPEEALMANQGMAREAKIAFFDIADEHGNLSTPSDFHRMLFPPAYEAGARIHSNSWGI-NVGEYLFDAMKIDQYAYEHQDFLIIYAAGN--YGEDGFH--SIGSPGNTKNVLTVGATESGPERQ-----SQDRNY-------------IAKFSSRGPTKDGRLKPDIVSPGSYIYSARSAGYNNGG-TCSYVGMAGTSMAAPLVAGAAALIRQYFKEGHYYAALRARGECGPPYQCEDV-----IQPSAPLLKALFVNSAVAVKALRLE--DELVALYHPPDLAQGHGRIKIDAVLDFYGHFSLFFADKQSLASGKSRTYYFQIAAASNASSVPLQVTLCWIDPPNSFTAQKQLLHDLDLHVLDPSGVRFYPN------------------------GLPGPDSKNNVEKIVVVEP-LPGGVYTLVVTAGELSEAEEQLYALVATGDGRAL 688          

BLAST of mRNA_D-herbacea_F_contig113.1036.1 vs. uniprot
Match: W7TM23_9STRA (Subtilisin n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TM23_9STRA)

HSP 1 Score: 262 bits (669), Expect = 1.700e-67
Identity = 213/677 (31.46%), Postives = 325/677 (48.01%), Query Frame = 0

Query:  325 DEATATACFLALVGFLSAQPEVMHIHPEAIPAPTNKVLTAYVEGGGDSS--TPMRTAGLDGSGEVIGITDTGLDDHSCFFRDDENGLVPRTTMSEASVYPDQRKVIQYVVYADDKD-ENGHGTHVSGIAAGAI---TDGWKAPWESQFTEAKC------------EDEGHVRSCLGSCVEEMVGGECH-WNQELSCPMSDCDEDTSCDDDTGYTFPCFGDPVDELPEASGMAPASKIAFFDIGTADDGLVIPDNIGDM-WDAQYAAGARIMTNSWSLVTVTEPSVRDVELDEWVHDHPDAFVVFGAGNNGYEDGGFNPGSVQSPATAKTAMTVGCSNSGPDRAYAYYTTASSNYEGVMWPEDDVSFEVSPFSARGPVGRFTAKPDVIAPGLMVHSARASRSEDGEETCALDSYGGTSMSAPAIAGAAALVRQYFRQGVFAKYLDDEGLCNSDGIHTEAGLCKAFEPSGYLVKAVLINSAEWLGNMQTVSYDDDAEWMTVLDFAQGFGAVQMASGMPTKDAPVGAFYHENALAT--DRLHYYSIYVADPSKDV--SVTVAWYDPPST-VASYNLLHDLDLFVMSVESRKYFRSNSYNTMLSMCADNETCANKYEFTFGNNLADYMNPVERISIPSSDLDEGYYVIGVRARTLTESDSQAYGLAASGGDLVLHDMAKYW 976
            DEA   AC   LV F+++ P+++ I   A  A  N   T  ++ G +++   P+   GL G GE+IG+ DTGLDD SCFF D+  G V R++         QRKV+QYV + D  D   GHGTHVSG  AG +    +G +  + ++     C            +D G+V     SC + +  G C  W    +CP+  CD      D  G    C  +P + L    GMA  +KIAFFDI      L  P +   M +   Y AGARI +NSW +  V E    D+++D++ ++H D  +++ AGN  Y + GF+  S+ SP   K  +TVG + SGP+R                    D  + ++ FS+RGP      KPD+++PG  ++SAR++   +G  TC+  +  GTSM+AP +AGAAAL+RQYF++G +   L   G C       +       +PS  L+KA+ +NSA  +  ++    D+        D AQG G +++ + +         F  + +LA+   R +Y+ I  A  +  V   VT+ W DPP++  A   LLHDLDL V+      ++ +                        G    D  N VE+I +    L  G Y + V A  L+E++ Q Y L A+G    L + + ++
Sbjct:  416 DEADRQACLEGLVAFIASHPDILSIQTRARYALQNDQATWIIQSGDEATHARPLWDLGLTGKGELIGVADTGLDDESCFFYDNALGQVARSSWEAPFTDMTQRKVVQYVSFVDGGDLVEGHGTHVSGSLAGVVEPRNEGRRGHFCAEGETLACTGMCLSAPTCQLKDYGYV-----SCQDWLQDGHCDAW---FACPLYGCDGG----DCVGLA--CNPNPEEALMANQGMAREAKIAFFDIADEHGNLSTPSDFHRMLFPPAYEAGARIHSNSWGI-NVGEYLFDDMKIDQYAYEHQDFLIIYAAGN--YGEDGFH--SIGSPGNTKNVLTVGATESGPERQ-----------------SQDRDY-IAKFSSRGPTKDGRLKPDIVSPGSYIYSARSAGYNNGG-TCSYVAMAGTSMAAPVVAGAAALIRQYFKEGHYYAALRARGECGPPYQCEDV-----IQPSAPLLKALFVNSAVPVKALRLE--DELVALYHPPDLAQGHGRIKIDAVLDFYGHFSLFFADKQSLASGESRTYYFQIAAASNASSVPLQVTLCWIDPPNSFTAQKQLLHDLDLHVLDPSGVLFYPN------------------------GLPGPDRKNNVEKIVVVEP-LPGGVYTLVVTAGELSEAEEQLYALVATGDGRALTEESVWY 1022          

BLAST of mRNA_D-herbacea_F_contig113.1036.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 249 bits (636), Expect = 1.470e-64
Identity = 221/749 (29.51%), Postives = 337/749 (44.99%), Query Frame = 0

Query:  296 EACGWDSNMSFVHARKDVMYLRGIHH---------ADHDEATA---TACFLALVGFLSAQPEVMHIHPEAIPAPTNKVLTAYVEGGGDSSTPMRTAGLDGSGEVIGITDTGLDDHSCFFRDD-----------ENGLVPRTTMSEASVYP-----------DQRKVIQYVVYAD-------------------------DKDENGHGTHVSGIAAGAITDGWKAPWESQFTEAKCEDEGHVRSCLGSCVEEMVGGECHWNQELS---------CPMSDCDEDTSCDDDTGYTFPCFGDPVDE-LPEASGMAPASKIAFFDIGTADDGLVIPDNIGD-MWDAQYAAGARIMTNSWSLVTVTEPSVRDVELDEWVHDHPDAFVVFGAGNNGYEDGGFNPGSVQSPATAKTAMTVGCSNSGPDRAYAYYTTASSNYEGVMWPEDDVSFE-VSPFSARGPVGRFTAKPDVIAPGLMVHSARASRSEDGEETCALDSYGGTSMSAPAIAGAAALVRQYFRQGVFAKYLDD---EGLCNSDGIHTEAGLCKAFEPSGYLVKAVLINSAEWLGNMQTVSYDDDAEWMTVLDFAQGFGAVQMASGMPTKDAPVGAFYHENALAT----DRLHYYSIYV-ADPSKDVSVTVAWYDPPSTVAS-YNLLHDLDLFVMSVESRKYFRSNSYNTMLSMCADNETCANKYEFTFGNNLADYMNPVERISIPSSDLDEGYYVIGVRARTLTESDSQAYGLAASG 964
            + C WD  ++ + A  D + + G+ H           H+E +     ACF+ LV F + +PEV+ + P       N+     ++    + TP+  AGLDG+GEVI + DTGLD+ SCFF DD           E   V  T  S+   YP           ++RKVIQY+   +                           D  GHGTH +G AAGA  +      +   T     DE     C+G C++E    +   +  +          CPM  CD         G T PC  D VDE L +  GMA  +K++ FD+     GL+  + +G+ +W+    AG ++ + S    T+  P   ++  D++++++P+  ++F AGN+G  +      ++ SPA  K  + VG S SG  R              V+  E     + V+ FS+ G       KP+V+APG MV+SA +  ++    +C L +Y GTSMS P +AGAAA+VRQYF    F  Y  D    G C SDG      +C+ F PS   VKA+LINSA  +G     S   DA         +GFG + +  GMP +     A +  +AL T    D +  Y   V AD   ++  T++W D P+T  S   L++DLDL V+S     Y                          +G+++ D  N  ER+ + + D++ G + + V + +L  +DSQ+Y L  +G
Sbjct:  272 DICSWDE-VALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTGSSQLIYYPVFEAGDFTYTEERRKVIQYIELVNPDSDYVAFSTTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLN------DPAETLTCSGDE--TLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCD---------GSTDPCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLI--EVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETERSVC--TMASPAIGKNVLAVGASTSGDTR--------------VISTEGGAGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDS--HSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETF--YTADVTARGFC-SDGF-----ICEGFSPSAATVKALLINSANLMGE----SSQPDAY--------RGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRM------------------------WGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLV-TDSQSYSLVVNG 937          

BLAST of mRNA_D-herbacea_F_contig113.1036.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 248 bits (634), Expect = 8.470e-64
Identity = 236/817 (28.89%), Postives = 347/817 (42.47%), Query Frame = 0

Query:  287 EGGEELPLSEACGWDSNMSFVHARKDVMYLRGIHH------------ADHDEATATACFLALVGFLSAQPEVMHIHPEAIPAPTNKVLTAYVEGGGDSSTPMRTAGLDGSGEVIGITDTGLDDHSCFFRDDENG--------------------------------------------------LV-----------------------------PRTTMSEASVYPD--------QRKVIQYVVYADDKDENGHGTHVSGIAAGAITDGWKAPWESQFTEAKCEDEGHVRSCLGSC--VEEMVGGE---CHWNQELS------------CPMSDCDEDTSCDDDTGYTFPCFGDPVDE-LPEASGMAPASKIAFFDIGTADDGLVIPDNIGDMWDAQYAAGARIMTNSWSLVTVTEPSVRDVELDEWVHDHPDAFVVFGAGNNGYEDGGFNPGSVQSPATAKTAMTVGCSNSGPDRAYAYYTTASSNYEGVMWPEDDVSFEVSPFSARGPVGRFTAKPDVIAPGLMVHSARASRSEDGEETCALDSYGGTSMSAPAIAGAAALVRQYFRQGVF-AKYLDDEGLCNSDGIHTEAGLCKAFEPSGYLVKAVLINSAEWLGNMQTVSYDDDAEWMTVLDFAQGFGAVQMASGMPTKDAPVGAFYHENALATDR----LHYYSIYV-ADPSKDVSVTVAWYDPPSTV-ASYNLLHDLDLFVMSVESRKYFRSNSYNTMLSMCADNETCANKYEFTFGNNLADYMNPVERISIPSSDL---------------DEGYYVIGVRARTLTESDSQAYGLAASG 964
            EGG  +   + C WD  +  VH   D++ ++G+ H                +    ACF+AL+ FL+++PEV+ + P       N      V+    + +PM  AGLDG+GEVI I DTGLD +SCFF D++ G                                                  LV                             P  T + ++ Y +        Q   I    +A+D D  GHGTH +G AAGA+           FTE  CE EG   SC+G C  V++  G +     W+ +              CP  DCD       D    + C GD   E L +  G+A  +++A FDI     G+ I      MW+    AG+++ +NSW +    +    DV  D++++ + D  ++F AGN+G +  G   G+   PA AK  + VG S SGP R  ++        +G    +D  +     FS+ GP      KP+V+APG M++SA  S   DG  TC L  Y GTSMS P +AGAAALVRQYF+   F A  +++ G C+S G       C+ F PS   VKA+LINSA  +G     S + D          +GFG V + +GMP       A +  ++  T      L  YS  V A    D+  T++W DPP+T  +S  L+HDLDL V S    +Y          SM  D                 D +N  ER+ +P+S++                +G + + V ++ L  +++Q+Y L  +G
Sbjct:  324 EGGPTI--GDVCSWDG-LVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAED-DAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEV-CE-EGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCD-------DLPEEY-CLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGDDWSGCTMGT---PALAKNVLAVGASTSGPGRL-SFTDEDGDPTDGDSRVQDIDAMAY--FSSFGPTLDNRIKPEVVAPGDMIYSAAGSG--DGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFCDSFG-------CEPFAPSAATVKAMLINSANLMGG----SAEPDG--------LRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRY----------SMWRDG--------------TVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTG 1075          

BLAST of mRNA_D-herbacea_F_contig113.1036.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 240 bits (613), Expect = 2.170e-61
Identity = 223/776 (28.74%), Postives = 337/776 (43.43%), Query Frame = 0

Query:  296 EACGWDSNMSFVHARKDVMYLRGIHHADHDEATA-------------TACFLALVGFLSAQPEVMHIHPEAIPAPTNKVLTAYVEGGGDSSTPMRTAGLDGSGEVIGITDTGLDDHSCFFRDDENG-------------LVPRTTMSEAS-------------------------------------VYPDQRKVIQYV--VYADDKD---------------------------ENGHGTHVSGIAAGAITDGWKAPWESQFTEAKCEDEGHVRSCLGSCVEEMVGGECHWNQELSCPMSDC-DEDTSC------DDDTGYTFPCFGDPVDE-LPEASGMAPASKIAFFDIGTADDGLVIPDNIGDMWDAQYAAGARIMTNSWSLVTVTEPSVRDVELDEWVHDHPDAFVVFGAGNNGYEDGGFNPGSVQSPATAKTAMTVGCSNSGPDRAYAYYTTASSNYEGVMWPEDDVSFEVSPFSARGPVGRFTAKPDVIAPGLMVHSARASRSEDGEETCALDSYGGTSMSAPAIAGAAALVRQYF-RQGVFAKYLDDEGLCNSDGIHTEAGLCKAFEPSGYLVKAVLINSAEWLGNMQTVSYDDDAEWMTVLDFAQGFGAVQMASGMPTKDAPVGAFYHENALATD-----RLHYYSIYVADPSKDVSVTVAWYDPPSTVAS-YNLLHDLDLFVMSVESRKYFRSNSYNTMLSMCADNETCANKYEFTFGNNLADYMNPVERISIPSSDLDEGYYVIGVRARTLTESDSQAYGLAASG 964
            + C W+  +S  HA  DV+ + G+ H  H    A              ACF+ LV FL+++PEV+ + P    A  N    A ++    + TP+  AGLDG+G+V+ + D+G+D+ SCFF  DE+G             + P  T S  S                                     ++PD+RK+IQY+  + +DD +                           E GHGTHV+G  AGA  +    P E       C+D     SC+G+CV++    +     +L+ P  +  D D  C      D D   T  C  D V E L    GMA  +K+A FD    + GL  P     +W+    A  +I +NS+           DV  D W+  +P+  ++F AGN+G      +  ++ SPA AK  + VG S SG  R  A   T     + V           + FS+ GP      KP+V+APG  ++SA +  S D   +C L +Y GTSMS P +AGA+A++RQYF  +  FA  +   G C+      E   C+AF PS   +KA++INSA  +G                 D ++GFG + + +GMP +     A +  ++  T      R  Y     A    D+ VT++W DPP+T  S   L+HDLDL V+S    +Y       TM                 + + + D  N  ER+ + + D++ G + + + A  LT +D Q+Y L  +G
Sbjct:  262 DICSWN-RVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLN---TPAEL----ISCKDT-ETMSCVGACVDDDEANDV---DDLATPNVEIVDLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLS-PFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERRR--STCTMSSPAIAKNVLAVGASTSGDTRLSA---TPGLGIDTV-----------AVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMD-SHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCD------EWAGCEAFSPSSATLKALMINSANLMGGSSEP------------DGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRY-------TM-----------------WASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLT-TDVQSYSLVVNG 964          

BLAST of mRNA_D-herbacea_F_contig113.1036.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 238 bits (606), Expect = 2.650e-61
Identity = 208/627 (33.17%), Postives = 305/627 (48.64%), Query Frame = 0

Query:  296 EACGWDSNMSFVHARKDVMYLRGIHHADH---------DEATA--TACFLALVGFLSAQPEVMHIHPEAIPAPTNKVLTAYVEGGGDSSTPMRTAGLDGSGEVIGITDTGLDDHSCFFRDDENGLVPRTTMSEASVYPDQRKVIQYVVYADDKDENGHGTHVSGIAAGAITDGWKAPWESQFTEAKCEDEGHVRSCLGSCVEEMVGGECHWNQELS----------------CPMSDCDE--DTSC-DDDTGYTFPCFGDPVDELPEASGMAPASKIAFFDIGTADDGLVIPDNIGD-MWDAQYAAGARIMTNSWSLVTVTEPSV--RDVELDEWVHDHPDAFVVFGAGNNGYEDGGFNPGSVQSPATAKTAMTVGCSNSGPDRAYAYYTTASSNYEGVMWPEDDVSFEVSPFSARGPVGRFTAKPDVIAPGLMVHSARASRSEDGEETCALDSYGGTSMSAPAIAGAAALVRQYFRQGVFAKYLDDEGLCNSDGIHTEAGL-CKAFEPSGYLVKAVLINSAEWLGNMQTVSYDDDAEWMTVLDFAQGFGAVQMASGMPTKDAPVGAFYHENALATDRLHYYS---IYVADPSK--DVSVTVAWYDPPSTVAS-YNLLHDLDLFVMS 882
            + C WDS +S  +A  D++ + G+ H  H         +EAT    ACF+ LV FLS + EV+ + P       N V    ++    +  P+  AGLDG+G+VI + DTGLD+ SCFF DD+   VP     EA       ++I     A D D  GHGTH +G A GA     + P E+  T +  E    V  C+G C++        W  +L                 CPM DCD+  D  C  DD G T          L +  GMA  +K+AFFD+    DG      +G+ +W+    AG +I  +S S+    E SV  +DV  D++++++P+  VV  AGN G  + G    ++ SPA  K A+ VG ++SG  R     T+A  +         DV   V+ FS+ GP      KP+V+APG  ++SAR+  S++   +C L +  GTSM+ P +AGA+A+VRQYF    F  Y+ D+    + G   ++G  C++F PS   VKA+LINSA  +G                 D  +GFG + +  GMP       A +  ++  T      S   I+  D +   D   T++W DPP++ AS   L+HDLDL V+S
Sbjct:  269 DICSWDS-ISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEAL-----GEIIPSSSSARD-DVEGHGTHCAGSALGATI---RTPAETPTTCSGTE----VPGCVGGCID---ADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATDPRCLSDDVGQT----------LADHGGMAQGAKLAFFDVFA--DGSSGTHFVGNGLWEPCLEAGCKI--HSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDGRTVCTMYSPAIGKNALAVGATSSGETRITK--TSADGDLIDGTNGYADVD-TVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDN--HSCRLSAKSGTSMACPIVAGASAMVRQYFMDESF--YVADQA---ARGWSDDSGRRCESFSPSSATVKALLINSANLMGGSSEP------------DGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVS 842          

BLAST of mRNA_D-herbacea_F_contig113.1036.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 235 bits (599), Expect = 8.100e-60
Identity = 225/816 (27.57%), Postives = 339/816 (41.54%), Query Frame = 0

Query:  294 LSEACGWDSNMSFVHARKDVMYLRGIHHA----DHDEATATACFLALVGFLSAQPEVMHIHPEAIPAPTNKVLTAYVEGGGDSSTPMRTAGLDGSGEVIGITDTGLDDHSCFFRDDENGLVPRTTMSEASVYPD------------------QRKVIQYV---------------------------------------------VYADDKDEN------------------------------------------GHGTHVSGIAAGAITDGWKAPWESQFTEAKCEDEGHVRSCLGSCVE----EMVGGECHWNQELSCPMSDCDEDTSCDDDTGYTFPCFGDPVDELPEASGMAPASKIAFFDIGTADDGLVIPDNIGDMWDAQYAAGARIMTNSWSLVTVTEPSVRDVELDEWVHDHPDAFVVFGAGNNG-YED-GGFNPGSVQSPATAKTAMTVGCSNSGP--------DRAYAYYTTASSNY--EGVMW---------PE--------DDVSFEVSPFSARGPVGRFTAKPDVIAPGLMVHSARASRSEDGEETCALDSYGGTSMSAPAIAGAAALVRQYFRQ-GVFAKYLDDEGLCNSDGIHTEAGLCKAFEPSGYLVKAVLINSAEWLGNMQTVSYDDDAEWMTVLDFAQGFGAVQMASGMPTKDA-PVGAFYHENALATDRLHYYSIYVADPSKDVSVTVAWYDPPSTVASYNLL-HDLDLFVMSVESRKYFRSNSYNTMLSMCADNETCANKYEFTFGNNLADYMNPVERISIPSSDLDEGYYVIGVRARTLTESDSQAYGLAASG 964
            + E CG+   +   H   D++ + G++H          T TAC++ L+  L+A+PEV+ + P       N V  A V+    + TP+  AGLDG+GEVI + D+GLD+ SCFF DD+   V    + +     D                  +RK++QY+                                              Y+D   E+                                          GHGT  +G AAGAI+       +S   E  C  +  +  C G C+     + +  +  ++ ++ CPM  CD +T    D   +     DPV+ L +  G+AP ++I+ FD+      +       D+W +    GA+I TNSW   T+ + +  +   D +++++P+  ++F AGN+G YED       +V SPA  K ++ VG ++SGP        D    Y     ++Y  EG  W         P         D V+F    FS++GP      KPDV+APG  V SA +S   DG  +C L +  GTS S P +AGAAALVRQYF+    FAK +   G C     H+ +  C  F PS   VKA++INSA  +G     +              +GFG V + +GMP       G    E A+A   +        D   ++  T+AW DPP+T  S   L HDLDL V++    KY       TM     D                 D +N VER+ +P   ++ G + + V A+ L  +D Q Y L  +G
Sbjct:  254 VGEICGF-GKLKLRHVADDLVLVEGMNHLLPGKRFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISA------QSILEEEACYGD-ELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYTCDGNT----DIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAF----FSSQGPTLDGRIKPDVVAPGDQVASA-SSDGTDGH-SCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAE---HSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNG------------VRGFGRVHLEAGMPMDGVGETGLSVVEAAIADGEITELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKY-------TMWESGVD-----------------DTVNVVERVIVPVESMESGVWSVVVSAKALL-TDEQLYSLVVTG 1011          

BLAST of mRNA_D-herbacea_F_contig113.1036.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 234 bits (596), Expect = 1.280e-59
Identity = 221/744 (29.70%), Postives = 335/744 (45.03%), Query Frame = 0

Query:  275 AALEHLGEEMAAEGGEELPLSEACGWDSNMSFVH-ARKDVMYLRGIHH---------ADHDEATA---TACFLALVGFLSAQPEVMHIHPEAIPAPTNKVLTAYVEG----GGDSSTPMRTAGLDGSGEVIGITDTGLDDHSCFFRDDENGLVPR----TTMSEASVYPDQRKVIQYVV--------YADDKDENGHGTHVSGIAAGAITDGWKAPWESQFTEAKCEDEGHVRSCLGSCVEEM--------VGGECHWNQ-ELSCPMSDCDEDTSCDDDTGYTFPCFGDPVDE-LPEASGMAPASKIAFFDI--GTADDGLVIPDNIGDMWDAQYAAGARIMTNSWSLVTVTEPSVRDVELDEWVHDHPDAFVVFGAGNNGYEDGGFNPGS--VQSPATAKTAMTVGCSNSGPDRAYAYYTTASSNYEGVMWPEDDVSFEVSPFSARGPVGRFTAKPDVIAPGLMVHSARASRSEDGEET--CALDSYGGTSMSAPAIAGAAALVRQYFRQGVFAKYLDD---EGLCNSDGIHTEAGLCKAFEPSGYLVKAVLINSAEWLGNMQTVSYDDDAEWMTVLDFAQGFGAVQMASGMPTKDAPVGAFYHENALATD----RLHYYSIYV-ADPSKDVSVTVAWYDPPSTVAS-YNLLHDLDLFVMSVESRKYFRSNSYNTMLSMCADNETCANKYEFTFGNNLADYMNPVERISIPSSDLDEGYYVIGVRARTLTESDSQAYGLAASG 964
            A++ H   E A+ G  ++     C W  N  FVH A  D + + G+ H           H+E +     ACF+ LV FL+ +PEV+ + P     PT +VL A   G       + TP+  AGLDG+GE+I + D+GLD+ SC+F DD    V        +  A+VY       +           Y  ++ + GHGTH +G AAGA  +          T+          SC+G+C++ +        +  +      +  CPM  CD  T    DT     C  D V E L    GMA  +K+A FD+  G  D    I +N+   W+    AG ++ +NS         +  D+  D++++++ +  ++F AGN    +GG    +  + +P   K ++ +G S SG  R  +  T  ++  + V           + FS+ G       KP+V+APG  V+SA +    DG +T  C L +Y GTSMS P +AGAAA++RQYF    F  Y  D    G C SDG      +C+ F PS   VKA+LINSA  +G                 D  +GFG V + +GMP +       +  +AL T      L  Y   V AD   D+  T++W DPP+T  S   L+H+LDL V+S +   Y                          +G++  D  N  ER+ + ++D+  G + + V A  L  +DSQ+Y L  +G
Sbjct:  254 ASVVHELSEAASTGPGDI-----CSW--NRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPR----PTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELGRAAVYSSSYSSDETATIFNGGDFSYYPERRKAGHGTHTAGSAAGATLNDPAETITCNVTD--------TLSCVGACIDLLDATDDLVSISSQTDGTDLDRLCPMYGCDNST----DTT----CLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSIGNNL---WEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGN----EGGIERSTCTINTPGIGKNSLAIGASTSGDTRVIS--TVGATGIDTV-----------AEFSSWGFTTDDRIKPEVVAPGDSVYSAAS----DGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETF--YTADVTARGFC-SDGF-----ICEGFSPSAATVKALLINSANLMGGSSEP------------DGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRM------------------------WGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLV-TDSQSYSLVVNG 901          

BLAST of mRNA_D-herbacea_F_contig113.1036.1 vs. uniprot
Match: A0A6H5JBB4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JBB4_9PHAE)

HSP 1 Score: 232 bits (591), Expect = 2.140e-59
Identity = 175/489 (35.79%), Postives = 242/489 (49.49%), Query Frame = 0

Query:  318 GIHHADHDEATATACFLALVGFLSAQPEVMHIHPEAIPAPT--NKVLTAYVEGGGDSSTPMRTAGLDGSGEVIGITDTGLDDHSCFFRDDENGLVPRT--------TMSEASVYPD--QRKVIQYV-------VYADDKDENGHGTHVSGIAAGAITDGWKAPWESQFTEAKCEDEGHVRSCLGSCVEEMVGGEC----HWNQELSCPMSDCDEDTSCDDDTGYTFPCFGDPVDE-LPEASGMAPASKIAFFDIGTADDGLVIPDNIGD-MWDAQYAAGARIMTNSWSLVTVTEPSVRD---VELDEWVHDHPDAFVVFGAGNNGYEDGGFNPGSVQSPATAKTAMTVGCSNSGPDRAYAYYTTASSNYEGVMWPEDDVSFEVSPFSARGPVGRFTAKPDVIAPGLMVHSARASRSEDGEETCALDSYGGTSMSAPAIAGAAALVRQYFRQGVFAKYLDDEGLCNSDGIHTEAGLCKAFEPSGYLVKAV 778
            G   A   E   TAC + LV FL+A+PEV+ +   A+P     N V    V+GG  ++ P+   G+DGSGEV+ + D+G+D+ SCFFRD    L P          T+       D  QRKV+QY+       VY++D D +GHG+HV+G  AG+I  GW  P           +E    SC+G C++  V  E      ++ +  CP  DCD          +   C  D   E L +A G+AP +K+A  D+G++D    +   +G  MW+     GARI + SW  +   EP   D   V  D+W +++P+  +VF AGN G +  G    S+ SPA +K  + VG S SG  R               + P D    EVS FS+ GP      KPD++APG  + SA    S  G+ +C LD   GTSMS P  AGAAALVRQY   G +A+ +   G   + G    A  C AF PSG LVK +
Sbjct:  412 GSGSASASEREKTACLMGLVSFLAARPEVVRV--SALPRAELFNAVAGRIVQGGSATTQPIWDRGVDGSGEVVQVVDSGIDEGSCFFRDSATTLGPDHGYLRDYDGTLGAGDYTYDLSQRKVVQYIESTRGDDVYSED-DPDGHGSHVAGSVAGSIYSGWTGPANCPDGVGDATEEQTAMSCVGVCLDPEVLEEYISDNTFDLDALCPEYDCD--------VYWEAACLSDDRSETLEDAKGVAPGAKLAVLDLGSSDS---LEQALGGTMWEVSAGTGARIHSASWGFID--EPCTVDDSSVSFDQWAYENPEHLLVFAAGNLGDDMTGC---SIASPAVSKNCLAVGSSMSGELR---------------LSPGD--MDEVSDFSSMGPTTDDRIKPDILAPGHYIFSA----SNGGDGSCQLDRNSGTSMSCPIAAGAAALVRQYLVDGFYAEDVGARGSLCAAGTDP-AWACDAFSPSGALVKVM 859