prot_D-herbacea_F_contig100.254.1 (polypeptide) Desmarestia herbacea DmunF female

BLAST of mRNA_D-herbacea_F_contig100.254.1 vs. uniprot
Match: D7FHY7_ECTSI (General transcription factor IIH subunit 4 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FHY7_ECTSI)

HSP 1 Score: 728 bits (1878), Expect = 9.590e-260
Identity = 378/500 (75.60%), Postives = 409/500 (81.80%), Query Frame = 0

Query:   39 MNTNVFDYLGRLPPEALDKLYG------QDAGQSDHGQWTCRALLQSLPQLAKQHVMRLLFVDGFVDKGMLKSWVKKEYQRVHTASVRKLVGLRLLLQSADGQEYRLNPPFRDNLQRALSASDKTPWQGNVPLTADRSPPTVAKIERQMHSQWQDVLYFLVGSDEEGRSIPPARVVKFMEESGLMRAVSRSGALRITDKGYEFMLKETHVQAWMVVHALINGYGRTQPGCRDDLLAFLFQLSYCKVGDAYPLEALTPTQRELAQDFVELGLLFKRKAKSKRFYPTSIAVNLIFGSVLA-DGGG------RAQQQTAGSVPVGKKEDDTTIQIIVETNFQVVAYTRSKLHYAMLSLFLELRARLPNVIIGAITRESMRRALATGIKGRQVLDFLKWHAHPVVRRRSPVVPENIADQILLWERERDRMVQSEGVLLDVSNAHDESYRRIVEFANGVGGLLWHSPFGASADGY-----------------NRKKLIIVTPRAV 508
            M+ NVFDYL RLPP+ALDKLYG         G   HGQWTCRALLQSLPQLAKQHVMRLLFV+G V KGMLKSWVKKEYQRVH ASVRKLV LR+LL SA GQEY+LNPPFR+NLQRAL ASDKTPWQG+VPL+ADRSPPTVAKIERQMHSQWQDVLYFLVGSDEEGRS PPARVVKFMEESGLMR V   GALRITDKGYEFMLKE HVQAWMVVHALINGYGRTQPGCRD+LLAFLFQLSYCKVGDAYPL ALT TQR+LAQDFVELGLLFKRKAKS RFYPTSIAVNLIFGS  + D GG      + + Q A      K+EDDT+I IIVETNFQV+AYTRSKLH+AMLSLFLELRA LPN I+GAITRESMR+AL+TGIKGRQVLDFLKWHAHPVVRRR+PVVPENIADQ+LLWERERDRM   +GVL+DVS A  +++R + EFAN   GLLW SP G                        RKKL++V+P  V
Sbjct:   19 MSLNVFDYLERLPPDALDKLYGAAPPAADGQGGRSHGQWTCRALLQSLPQLAKQHVMRLLFVEGPVGKGMLKSWVKKEYQRVHAASVRKLVSLRVLLPSAGGQEYQLNPPFRENLQRALCASDKTPWQGDVPLSADRSPPTVAKIERQMHSQWQDVLYFLVGSDEEGRSTPPARVVKFMEESGLMRPVRGRGALRITDKGYEFMLKEAHVQAWMVVHALINGYGRTQPGCRDELLAFLFQLSYCKVGDAYPLGALTQTQRDLAQDFVELGLLFKRKAKSTRFYPTSIAVNLIFGSSPSGDAGGAXXXXTQRKPQPAXXXXXXKREDDTSIHIIVETNFQVIAYTRSKLHFAMLSLFLELRALLPNAIVGAITRESMRKALSTGIKGRQVLDFLKWHAHPVVRRRTPVVPENIADQVLLWERERDRMEHRDGVLVDVSYASRDAFRGMTEFANAKQGLLWSSPLGGEDXXXXXXXXXXXXXXXXXXLAGRKKLMVVSPGIV 518          

BLAST of mRNA_D-herbacea_F_contig100.254.1 vs. uniprot
Match: A0A6H5KJZ4_9PHAE (General transcription factor IIH subunit 4 n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KJZ4_9PHAE)

HSP 1 Score: 495 bits (1274), Expect = 5.470e-170
Identity = 266/369 (72.09%), Postives = 279/369 (75.61%), Query Frame = 0

Query:   39 MNTNVFDYLGRLPPEALDKLYG------QDAGQSDHGQWTCRALLQSLPQLAKQHVMRLLFVDGFVDKGMLKSWVKKEYQRVHTASVRKLVGLRLLLQSADGQEYRLNPPFRDNLQRALSASDKTPWQGNVPLTADRSPPTVAKIERQMHSQWQDVLYFLVGSDEEGRSIPPARVVKFMEESGLMRAVSRSGALRITDKG------------------------------------------------YEFMLKETHVQAWMVVHALINGYGRTQPGCRDDLLAFLFQLSYCKVGDAYPLEALTPTQRELAQDFVELGLLFKRKAKSKRFYPTSIAVNLIFGSVLAD-----GGGRAQQ--QTAGSVPV 346
            M+ NVFDYL RLPP+ALDKLYG      Q  G   HGQWTCRALLQSLPQLAKQHVMRLLFV+G V KGMLKSWVKKEYQRVH ASVRKLV LR+LL SADGQEY+LNPPFR+NLQRAL ASDKTPWQG+VPL+ADRSPPTVAKIERQMHSQWQDVLYFLVGSDEEGRS PPARVVKFMEESGLMR V   GALRITDKG                                                YEFMLKE HVQAWMVVHALINGYGRTQPGCRD+LLAFLFQLSYCKVGDAYPL ALT TQR+LAQDFVELGLLFKRKAKS RFYPTSIAVNLIFGS  +      GGG AQ+  Q AG + V
Sbjct:   19 MSLNVFDYLERLPPDALDKLYGAAPPAAQGQGGRSHGQWTCRALLQSLPQLAKQHVMRLLFVEGPVGKGMLKSWVKKEYQRVHAASVRKLVSLRVLLPSADGQEYQLNPPFRENLQRALCASDKTPWQGDVPLSADRSPPTVAKIERQMHSQWQDVLYFLVGSDEEGRSTPPARVVKFMEESGLMRPVRGGGALRITDKGQEEAWNCSRGARGGSFLCLRGGVGRGGARVLSTSSMLEGAVYPRRTGLYEFMLKEAHVQAWMVVHALINGYGRTQPGCRDELLAFLFQLSYCKVGDAYPLGALTQTQRDLAQDFVELGLLFKRKAKSTRFYPTSIAVNLIFGSSPSGDAGGAGGGGAQRKPQPAGGLGV 387          

BLAST of mRNA_D-herbacea_F_contig100.254.1 vs. uniprot
Match: A0A836C856_9STRA (General transcription factor IIH subunit 4 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C856_9STRA)

HSP 1 Score: 358 bits (920), Expect = 2.390e-115
Identity = 215/481 (44.70%), Postives = 282/481 (58.63%), Query Frame = 0

Query:   95 DGFVDKGMLKSWVKKEYQRVHTASVRKLVGLRLLLQS-------ADGQEYR-----LNPPFRDNLQRALSASDKTPWQGN--VPLTADRSPPTVAKIERQMHSQWQDVLYFLVGSDEEGRSIPPARVVKFMEESGLMRAVSRSGALRITDKGYEFMLKETHVQAWMVVHALINGYGRTQPGCRDDLLAFLFQLSYC------------------------------------------KVGDAYPLEALTPTQRELAQDFVELGLLFKRKAKSKRFYPTSIAVNLIFGSVLADGGGRAQQQTAGSVPVGKKEDDTTIQIIVETNFQVVAYTRSKLHYAMLSLFLELRARLPNVIIGAITRESMRRALATGIKGRQVLDFLKWHAHPVVRRRSPVVPENIADQILLWERERDRMVQSEGVLLDVSNAHDESYRRIVEFANGVGGLLWHSPFGASADGYNRKKLIIVTPRAVGAVTEFVERTM 519
            DG + K +LK WV+  Y  VH A+VR+L  LR+L+++       A  Q+ R     LNPPFR NL +AL   D  PW+ +    L  D+  P+ AKIE +M  +W+ VL+FLV SD  G    P+ V  FM+++GLM    RS  L IT++GYEFML++ HVQAWM V +LI     +    RDDLL+FLF LSYC                                          KV + YPL ALTP Q +LA+DF++LGLL++RK  S+RFY T IAVNL+FG+  A+   RA  Q A +    + +    + IIVETNFQVVAYT S LH AML+LFLELRARLPN++IG ITRES+R+AL TGIK  Q+L+FLKWHAHP V  R P++PENIADQI+LWERER R+    G LLD+       + ++ E+A  +G  LW +           K+++IV   AV  V  F +  M
Sbjct:   22 DGAISKDLLKGWVRDAYTGVHRAAVRQLSALRVLVRAQELSGAAAAAQQQRRGMIALNPPFRKNLLQALLTPDVAPWKDDPRFALRPDKLTPSRAKIEGEMRRKWEAVLHFLVASDGAGAGPTPS-VRAFMQQAGLMARRGRSEQLVITNRGYEFMLRDIHVQAWMFVLSLIRSKS-SDAQARDDLLSFLFMLSYCTVSAALQPLEGHHNAQSVCAAPSLSCKPLPAAPSASVNRCSEKVCEDYPLLALTPAQAQLARDFIDLGLLYQRKPSSQRFYTTEIAVNLVFGAAAAEHPSRAVIQAADAS--AQLDLAPRMTIIVETNFQVVAYTTSDLHVAMLTLFLELRARLPNMVIGVITRESVRQALVTGIKASQILNFLKWHAHPNVINRRPMIPENIADQIILWERERTRVKYDAGTLLDLPFEGVHDFDKVAEYAEELGACLWAA---------REKRVLIVAAAAVNKVVAFADGLM 489          

BLAST of mRNA_D-herbacea_F_contig100.254.1 vs. uniprot
Match: A0A024TBA0_9STRA (General transcription factor IIH subunit 4 n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024TBA0_9STRA)

HSP 1 Score: 333 bits (855), Expect = 7.430e-106
Identity = 197/461 (42.73%), Postives = 279/461 (60.52%), Query Frame = 0

Query:   42 NVFDYLGRLPPEALDKLYGQDAGQSDHGQWTCRALLQSLPQLAKQHVMRLLFVD-GFVDKGMLKSWVKKEYQRVHTASVRKLVGLRLLLQSADGQEYRLNPPFRDNLQRALSASDKTPWQ-GNVPLTADRSPPTVAKIERQMHSQWQDVLYFLVGSDEEGRSIPPARVVKFMEESGLMR-AVSRSGALRITDKGYEFMLKETHVQAWMVVHALINGYGRTQPGCRDDLLAFLFQLSYCKVGDAYPLEALTPTQRELAQDFVELGLLFKRKAKSKRFYPTSIAVNLIFGSVLADGGGR-------------AQQQTAGSVP-VGKKEDDTTIQIIVETNFQVVAYTRSKLHYAMLSLFLELRARLPNVIIGAITRESMRRALATGIKGRQVLDFLKWHAHPVVRRRSPVVPENIADQILLWERERDRMVQSEGVLLDVSNAHDESYRRIVEFANGVGGLLWH 485
            N F++LG LP  ++D+LY QDA       W C+A+ QSL  LA+Q VMRLLF +        +  WV+   Q   TA++ KL  LR+L       +Y LNP F++ L++ALS+   +PW+ G   L A++  P V ++E     +W  VL+F+VGS     + PPA V+  +E + LM+ + S S AL ITD GYEFMLK+ H Q W+ V   +    R      D++L FLFQ+SYC++ + Y +  LT  Q++L  D VELGLL++R AK+ RFY TS+AVNLIFG +L                   A   +   +P       D+++ I+VETNF+V AYT+S LH AMLS+F+++ ARLPN+ +G +TRES+R AL+ GI   Q+ DFL  HAHP +   SPV+PENIADQ+ LW+RER+R+    G L+DV+N   E +  +V+ A  +G LLWH
Sbjct:    2 NAFEFLGSLPGGSVDRLY-QDA-------WACQAVFQSLSPLAQQIVMRLLFSNHATYAHDAILHWVQDAAQDKMTAAIEKLRHLRVLRVVQGSLDYVLNPVFQEQLKKALSSLGGSPWEAGRSKLPAEKPIPAV-ELELYARKRWDSVLHFMVGSTAV--AAPPATVIGILEHTKLMQPSKSDSRALHITDTGYEFMLKDIHAQTWIFVLEYMKDIDRKGILPADEMLQFLFQMSYCRMNEYYAVADLTRNQQQLLGDLVELGLLYRRGAKATRFYTTSLAVNLIFGGMLGQTRANMTMSQGLDHITNYAADPSKSRLPSASPARADSSLLIVVETNFKVYAYTQSSLHIAMLSVFVDIVARLPNLAVGFLTRESIRSALSNGISAEQIYDFLMQHAHPKMLGNSPVIPENIADQLYLWQRERNRIKFEPGELVDVAN---EDFDAVVKHAEDMGVLLWH 448          

BLAST of mRNA_D-herbacea_F_contig100.254.1 vs. uniprot
Match: W4FDT1_9STRA (General transcription factor IIH subunit 4 n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=W4FDT1_9STRA)

HSP 1 Score: 333 bits (854), Expect = 1.020e-105
Identity = 192/465 (41.29%), Postives = 276/465 (59.35%), Query Frame = 0

Query:   42 NVFDYLGRLPPEALDKLYGQDAGQSDHGQWTCRALLQSLPQLAKQHVMRLLFVD-GFVDKGMLKSWVKKEYQRVHTASVRKLVGLRLLLQSADGQEYRLNPPFRDNLQRALSASDKTPWQGNVPLTADRSPPTVAKIERQMHSQWQDVLYFLVGSDEEGRSIPPARVVKFMEESGLMR-AVSRSGALRITDKGYEFMLKETHVQAWMVVHALINGYGRTQPGCRDDLLAFLFQLSYCKVGDAYPLEALTPTQRELAQDFVELGLLFKRKAKSKRFYPTSIAVNLIFGSVLADGGGRAQQQTAGSVPVGKKED-------------------DTTIQIIVETNFQVVAYTRSKLHYAMLSLFLELRARLPNVIIGAITRESMRRALATGIKGRQVLDFLKWHAHPVVRRRSPVVPENIADQILLWERERDRMVQSEGVLLDVSNAHDESYRRIVEFANGVGGLLWH 485
            N F++LG LP  ++D+LY QDA       W C+A+ QS+  LA+Q VMRLLF + G      +  WV+   Q   TA++ KL  LR+L  +    EY LNP F+D L++ALS+   +PW+          P   A++E+    +W  VL+F+VGS     + PP  V+  +E +GLM+ + + + AL ITD GYEFMLK+ H Q W+ V   +    R      +++L FLFQ+SYC++ + Y +  LT  Q++L  D VELGLL++R AK+ RFY TS+AVNLIFG +L         QT  S+ + +  D                   D  + I+VETNF+V AYT+S LH AMLS+F+++ ARLPN+ +G +TRES+R AL+ GI   Q+ DFL  HAHP +   SPV+PENIADQ+ LW+RER+R+    G L+D      E +  +++FA  VG +LW+
Sbjct:    2 NAFEFLGSLPGGSVDRLY-QDA-------WACQAVFQSMSPLAQQIVMRLLFTNQGSYSHDAILQWVQDPAQVKMTAAIEKLRHLRVLRMAHGTAEYVLNPVFQDQLKKALSSLGGSPWEAGRHKLPSEKPIAAAELEQYARKRWDAVLHFMVGSTAV--AAPPPTVIGILEHTGLMQPSKTDARALHITDTGYEFMLKDIHAQTWIFVLEYMKDIDRKGILSAEEMLQFLFQMSYCRMNEYYAVADLTRHQQQLLGDLVELGLLYRRGAKATRFYTTSLAVNLIFGGMLG--------QTRSSITMSQGLDHLHRPSKSPHLPPPTAAAADAHLLIVVETNFKVYAYTQSSLHIAMLSVFVDIVARLPNLAVGFLTRESIRSALSNGISAEQIYDFLMQHAHPKMLGNSPVIPENIADQLYLWQRERNRIKFDAGELVD-GFVTTEDFDVVLKFAQDVGVMLWY 447          

BLAST of mRNA_D-herbacea_F_contig100.254.1 vs. uniprot
Match: A0A6G0WGX7_9STRA (General transcription factor IIH subunit 4 n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WGX7_9STRA)

HSP 1 Score: 326 bits (836), Expect = 5.040e-103
Identity = 191/488 (39.14%), Postives = 277/488 (56.76%), Query Frame = 0

Query:   42 NVFDYLGRLPPEALDKLYGQDAGQSDHGQWTCRALLQSLPQLAKQHVMRLLFVDGFVDKGMLKSWVKKEYQRVHTASVRKLVGLRLLLQSADGQEYRLNPPFRDNLQRALSASDKTPWQGNVPLTADRSPPTVAKIERQMHSQWQDVLYFLVGSDEEGRSIPPARVVKFMEESGLMR-AVSRSGALRITDKGYEFMLKETHVQAWMVVHALINGYGRTQPGCRDDLLAFLFQLSYCKVGDAYPLEALTPTQRELAQDFVELGLLFKRKAKSKRFYPTSIAVNLIFGSV---------LADGGGRAQQQTAGSV---PVGKKEDDTTIQIIVETNFQVVAYTRSKLHYAMLSLFLELRARLPNVIIGAITRESMRRALATGIKGRQVLDFLKWHAHPVVRRRSPVVPENIADQILLWERERDRMVQSEGVLLDVSNAHDESYRRIVEFANGVGGLLWHSPFGASADGYNRKKLIIVTPRAVGAVTEFVE 516
            N F+YLG LP  +LD+LY QD        W C+A+ QS+  L++Q VMRLLF    V K  L  W++   Q   TA++ KL  LR+L       ++ LNP F++ L+ ALS+   +PW+          P   A +E+    +W  VL+F+VGS       PP  V+  +E + LM+ + + + AL ITD GYEFMLK+ H Q W+ V   I    R      D++L F FQ+SYC++ + Y +  LT  Q++L  D V+LGLL+++ +K+ RFY TS+AVNLIFG +         +        QQ +  V   P       + + I+VETNF+V AYT S LH AMLS+F+++ ARLPN+ +G +TRES+R AL  GI   Q+ DFL  HAHP +   SPV+PENIADQ+ LW+RER+R+    G LLD      E +  +V++A  +  L+WH       D  + K  +++T     AV +F++
Sbjct:    2 NAFEYLGALPGTSLDRLY-QDP-------WACQAVFQSMNPLSQQIVMRLLFTTTHVSKDALMHWIQDSAQSKMTAAIEKLCHLRVLRPVQGTNKFGLNPAFQEKLKTALSSLGGSPWEAGRKNLPPEKPIAAADLEQYARKRWDAVLHFMVGSTAV--PAPPKEVIGILEHTRLMKPSETDARALHITDTGYEFMLKDIHAQTWIFVLNYIKDIDRKGILSADEMLQFFFQMSYCRMNEYYAVADLTRHQQQLLGDLVQLGLLYRKDSKATRFYTTSLAVNLIFGGMAGQTRTNITMVQSLEAVHQQASSQVVPRPFASTSSASHLLIVVETNFKVYAYTESTLHIAMLSVFVDIVARLPNLAVGYLTRESIRSALIHGISAEQIYDFLMQHAHPKMLANSPVIPENIADQLYLWQRERNRIKFDAGELLD-GFVTTEDFDSVVKYAQDLHVLMWH-------DAVHLK--LVITKAGGDAVRQFMQ 469          

BLAST of mRNA_D-herbacea_F_contig100.254.1 vs. uniprot
Match: D0NRC9_PHYIT (General transcription factor IIH subunit 4 n=21 Tax=Phytophthora TaxID=4783 RepID=D0NRC9_PHYIT)

HSP 1 Score: 325 bits (832), Expect = 2.860e-102
Identity = 198/468 (42.31%), Postives = 270/468 (57.69%), Query Frame = 0

Query:   42 NVFDYLGRLPPEALDKLYGQDAGQSDHGQWTCRALLQSLPQLAKQHVMRLLFVDGFVDKGMLKSWV------KKEYQRVHTASVRKLVGLRLLLQSADGQEYRLNPPFRDNLQRALSASDKTPWQ-GNVPLTADRSPPTVA-KIERQMHSQWQDVLYFLVGSDEEGRSIPPARVVKFMEESGLMRAVSR-SGALRITDKGYEFMLKETHVQAWMVVHALINGYGRTQPGCRDDLLAFLFQLSYCKVGDAYPLEALTPTQRELAQDFVELGLLFKRKAKSKRFYPTSIAVNLIFGSVLAD-----------GGGRA--QQQTAGSVPVGKKEDDTTIQIIVETNFQVVAYTRSKLHYAMLSLFLELRARLPNVIIGAITRESMRRALATGIKGRQVLDFLKWHAHPVVRRRSPVVPENIADQILLWERERDRMVQSEGVLLDVSNAHDESYRRIVEFANGVGGLLWHSP 487
            + FD+L  LPP  L++LY QD        W C+A+ Q+LP LA+Q VMRLL  +  V + +L+ WV       K       A+++KL GLR+ +Q   G  Y+ +P F+  L  ALS    +PW+ G + L  D      A  +ER   ++W  VL+++VGS       PP  VV  +  + L++A    S AL ITD GYEFMLK+ HVQ W+ +   I     T    ++D+L FLFQ+SYC+ G  Y +  LT TQR L  DF++ GLLF+++  S RFY TS+AVNLIFG                 G RA  + Q A        +    + ++VETNF++ AYT S LH AMLS+F+++ ARLPN+ IG ITRES+R AL  GI  +Q+ DFL  HAHP +RR SPV+PENIADQI LWERER+R+   EG+L D  N   E Y  + ++A G+  L W  P
Sbjct:    2 DAFDFLETLPPATLERLY-QDP-------WACQAIFQALPSLAQQFVMRLLPTNSAVPRDLLEQWVVPIPGETKRMPPQFNAALQKLEGLRVFVQQDGG--YKPHPTFQKQLMYALSNLGGSPWERGRLHLPRDPENTFAAVDLERYARARWDLVLHYMVGSTAVQE--PPQSVVDILLRTKLLQASGADSRALHITDTGYEFMLKDIHVQMWIFMLEYIRTLDNTGTLKQEDILQFLFQISYCQTGGYYAVADLTETQRLLLGDFIDFGLLFRKRQNSDRFYTTSLAVNLIFGGSTGQKRSHVSLTSSFAGVRAGMKSQVADPRQAPTVDHGARLLVVVETNFKIYAYTSSTLHVAMLSVFVDIVARLPNLAIGFITRESLRSALIHGISAQQIYDFLMKHAHPKMRRNSPVIPENIADQIYLWERERNRVQFLEGILFDGFNTK-EDYESVRDYAKGLMVLTWSDP 456          

BLAST of mRNA_D-herbacea_F_contig100.254.1 vs. uniprot
Match: A0A485KU14_9STRA (General transcription factor IIH subunit 4 n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KU14_9STRA)

HSP 1 Score: 320 bits (819), Expect = 1.870e-100
Identity = 189/461 (41.00%), Postives = 266/461 (57.70%), Query Frame = 0

Query:   42 NVFDYLGRLPPEALDKLYGQDAGQSDHGQWTCRALLQSLPQLAKQHVMRLLFVD--GFVDKGMLKSWVKKEYQRVHTASVRKLVGLRLLLQSADGQEYRLNPPFRDNLQRALSASDKTPWQGNVPLTADRSPPTVAKIERQMHSQWQDVLYFLVGSDEEGRSIPPARVVKFMEESGLMRAVSRSG-ALRITDKGYEFMLKETHVQAWMVVHALINGYGRTQPGCRDDLLAFLFQLSYCKVGDAYPLEALTPTQRELAQDFVELGLLFKRKAKSKRFYPTSIAVNLIFGSVLADG--------------GGRAQQQTAGSVPVGKKEDDTTIQIIVETNFQVVAYTRSKLHYAMLSLFLELRARLPNVIIGAITRESMRRALATGIKGRQVLDFLKWHAHPVVRRRSPVVPENIADQILLWERERDRMVQSEGVLLDVSNAHDESYRRIVEFANGVGGLLWH 485
            N F++L  LP  ++D+LY QDA       W C+A+ QS+  L +Q VMRLLF     +  + +L+ WV++  Q+    ++ KL  LR+L      +E+ LNP F++ L++ALS+   +PW+      A   P +   +E+    +W  VL+F+VGS       PP  V+  +E + LM+  S    AL ITD GYEFMLK+ H Q W+ V   I    R      +++L FLFQ+SYC++ + Y +  LT  Q++L  D VELGLLF++  K+ RFY TS+AVNLIFG VL                     QQ T G+VP         + I+VETNF+V AYT S LH AMLS+F+++ ARLPN+ +G +TRES+R AL  GI   Q+ DFL  HAHP +   SPV+PENIADQ+ LW+RER+R+    G LLD      E +  +V +A  +  LLWH
Sbjct:    2 NAFEFLATLPAASVDRLY-QDA-------WACQAVFQSMEPLGQQIVMRLLFTSQVSYSQESLLE-WVQEPAQQKMIHAIEKLSHLRVLRVVKGQREFVLNPVFQEQLKKALSSLGGSPWEAGRQKLAAEPPISALDLEQYARKRWDAVLHFMVGSTAV--PAPPPTVIGILEHTRLMQPSSSDARALHITDTGYEFMLKDVHAQTWIFVLEYIKDIDRRGILSAEEMLQFLFQMSYCRMNEYYAIGDLTRNQQQLLGDLVELGLLFRKGTKATRFYTTSLAVNLIFGGVLGQTRTSISMVQSLEQVHSASGQQLTKGAVP-RPVAVAAHLLIVVETNFKVYAYTESTLHIAMLSVFVDIVARLPNLAVGYLTRESIRSALIHGISAEQIYDFLMQHAHPKMLANSPVIPENIADQLYLWQRERNRIKFDAGELLD-GFVTTEDFDTVVNYAQDLDVLLWH 449          

BLAST of mRNA_D-herbacea_F_contig100.254.1 vs. uniprot
Match: A0A3M6VGH3_9STRA (General transcription factor IIH subunit 4 n=1 Tax=Peronospora effusa TaxID=542832 RepID=A0A3M6VGH3_9STRA)

HSP 1 Score: 317 bits (813), Expect = 1.980e-99
Identity = 197/469 (42.00%), Postives = 269/469 (57.36%), Query Frame = 0

Query:   42 NVFDYLGRLPPEALDKLYGQDAGQSDHGQWTCRALLQSLPQLAKQHVMRLLFVDGFVDKGMLKSWVKKEYQRV-------HTASVRKLVGLRLLLQSADGQEYRLNPPFRDNLQRALSASDKTPWQ-GNVPLTAD-RSPPTVAKIERQMHSQWQDVLYFLVGSDEEGRSIPPARVVKFMEESGLMRAVSR-SGALRITDKGYEFMLKETHVQAWMVVHALINGYGRTQPGCRDDLLAFLFQLSYCKVGDAYPLEALTPTQRELAQDFVELGLLFKRKAKSKRFYPTSIAVNLIFGSVLAD-----------GGGRA--QQQTAGSVPVGKKEDDTTIQIIVETNFQVVAYTRSKLHYAMLSLFLELRARLPNVIIGAITRESMRRALATGIKGRQVLDFLKWHAHPVVRRRSPVVPENIADQILLWERERDRMVQSEGVLLDVSNAHDESYRRIVEFANGVGGLLWHSP 487
            + FD+L  LPP  L++LY           W C+A+ Q+LP LA+Q V+RLL  +  V   +L+ WV  E   V       H A + KL GLR+ LQ  DG  YR +P F+  L  ALS    +PW+ G + L  D  +  T A +ER   ++W  VL+++VGS       PP  VV  +  + L++A    S AL ITD GYEFMLK+ HVQ W+ +   I     T    ++D+L FLFQ+SYC+ G+ Y +  LT TQR L  DF++ GLL++++  S RFY TS+AVNLIFG                 G RA  + Q A        +    + ++VETNF++ AYT S LH AMLS+F+++ ARLPN+ IG ITRES+R AL  GI  +Q+ DFL  HAHP +R  +PV+PENIADQI LWERER+R+    G+L D  N   E Y  + ++A G+  L W  P
Sbjct:    2 DAFDFLETLPPATLERLYEDP--------WACQAIFQALPPLAQQFVIRLLPTNAAVPCELLEQWVVPEPGEVKRMPPQFHVA-LHKLKGLRVFLQQ-DGS-YRSHPIFQKQLMYALSNLGGSPWESGRLCLPKDLENMFTTADLERYARARWDAVLHYMVGSTAVQE--PPQSVVDILIRTKLLQASGADSRALHITDTGYEFMLKDIHVQMWIFLLEYIRTLDNTGTLKQEDILQFLFQISYCQTGEYYAVADLTETQRLLLGDFIDFGLLYRKRTNSDRFYTTSLAVNLIFGGSTGQKRNHVSLSSSFAGVRAGMKSQMANPRQTPALDRGAQLLVVVETNFKIYAYTTSTLHVAMLSVFVDIVARLPNLAIGFITRESLRSALIHGISAQQIYDFLIKHAHPKMRHNTPVIPENIADQIYLWERERNRVQFMAGILFDGFNTK-EDYESVRDYAKGLKVLTWSDP 456          

BLAST of mRNA_D-herbacea_F_contig100.254.1 vs. uniprot
Match: A0A0P1AW06_PLAHL (General transcription factor IIH subunit 4 n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1AW06_PLAHL)

HSP 1 Score: 316 bits (810), Expect = 6.250e-99
Identity = 196/470 (41.70%), Postives = 271/470 (57.66%), Query Frame = 0

Query:   42 NVFDYLGRLPPEALDKLYGQDAGQSDHGQWTCRALLQSLPQLAKQHVMRLLFVDGFVDKGMLKSWV------KKEYQRVHTASVRKLVGLRLLLQSADGQEYRLNPPFRDNLQRALSASDKTPWQ-GNVPLTADRSPPTVAK-IERQMHSQWQDVLYFLVGSDEEGRSIPPARVVKFMEESGLMRAVSR-SGALRITDKGYEFMLKETHVQAWMVVHALINGYGRTQPGCRDDLLAFLFQLSYCKVGDAYPLEALTPTQRELAQDFVELGLLFKRKAKSKRFYPTSIAVNLIFGSVLADGGGRAQQQTAGS---VPVGKKEDDTT------------IQIIVETNFQVVAYTRSKLHYAMLSLFLELRARLPNVIIGAITRESMRRALATGIKGRQVLDFLKWHAHPVVRRRSPVVPENIADQILLWERERDRMVQSEGVLLDVSNAHDESYRRIVEFANGVGGLLWHSP 487
            + F++L  LP   L++LY QD        W C+A+ Q+LP LA+Q VMRLL  +  V + +L+ WV        E      A++ KL GLR+ +Q   G  YR +P F+  L  ALS    +PW+ G + L  D      AK +ER   ++W  VL+++VGS       PP  VV  +  + L+++    S AL ITD GYEFMLK+ HVQ W  +   I     T    ++D+L FLFQLSYC+ G  Y +  LT TQ+ L  DF++ GLLF++++ S RFY TS+AVNLIFG   + G  R+  +   S   V  G K   T             + ++VETNF++ AYT S LH AMLS+F+++ ARLPN+ IG ITRES+R AL  GI  +Q+ DFL  HAHP +R+ +P+VPENIADQI LWERER+R+   EG+L D  N   E Y  + ++A  +  L W  P
Sbjct:    2 DAFEFLETLPAATLERLY-QDP-------WACQAVFQALPPLAQQFVMRLLPTNTSVPRDLLEKWVVPLPGDSHEMPPQFNAALHKLDGLRVFIQENGG--YRPHPLFQKQLMYALSNLGGSPWERGRLRLPKDPENTFAAKDLERYARARWDSVLHYMVGSTAVQE--PPQSVVDILLRTKLLQSSEADSRALHITDTGYEFMLKDIHVQMWTFMLEYIRTLDNTGTLKQEDILQFLFQLSYCQTGGYYAVADLTQTQQLLLGDFIDFGLLFRKRSNSDRFYTTSLAVNLIFGG--SKGQERSHVRLTSSFAGVRAGVKSQVTNPRLTPVLDHEAQLLVVVETNFKIYAYTSSTLHVAMLSVFVDIVARLPNLAIGFITRESLRSALIHGISAQQIYDFLVKHAHPKMRQNTPIVPENIADQIYLWERERNRVQFIEGILFDGFNTK-EDYESVCDYARNLDVLTWADP 456