mRNA_D-dudresnayi_contig9796.28602.1 (mRNA) Desmarestia dudresnayi DdudBR16 monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_D-dudresnayi_contig9796.28602.1
Unique NamemRNA_D-dudresnayi_contig9796.28602.1
TypemRNA
OrganismDesmarestia dudresnayi DdudBR16 monoicous (Desmarestia dudresnayi DdudBR16 monoicous)
Homology
BLAST of mRNA_D-dudresnayi_contig9796.28602.1 vs. uniprot
Match: A0A7S3H5S6_9STRA (Hypothetical protein n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3H5S6_9STRA)

HSP 1 Score: 293 bits (750), Expect = 2.240e-98
Identity = 144/172 (83.72%), Postives = 160/172 (93.02%), Query Frame = 2
Query:   17 KMSTSIAANSPIPDQIVDIVEPVSGDYVIKAGQNFGEILRAHKKAVIFAVPGAFTPTCSEKHLPGFIERAHSMREAGVEEIYCLSVNDLFVMKAWGKSYATFADSGIKLVADGNADYSKAIGMELDLSAHRLGLRCKRFAAIVENGVFTAVEVDDSGLDKSSAESILAILRK 532
            KMS S+  NSPIP  +VDIVEPV+G+YV+KAGQ FG+IL AHKKAVIFAVPGAFTPTCSEKHLPGFIERAH+ R AGVEEIYCLSVNDLFVMKAWGKSY TFADSGIKLVADGN DY++A+G+ELDLSAHRLGLRCKRFAAIVENGVFT +E+DD+GL+KSSAE+ILAILRK
Sbjct:   13 KMSVSV--NSPIPAGVVDIVEPVNGEYVVKAGQAFGDILSAHKKAVIFAVPGAFTPTCSEKHLPGFIERAHNFRAAGVEEIYCLSVNDLFVMKAWGKSYETFADSGIKLVADGNGDYTRALGLELDLSAHRLGLRCKRFAAIVENGVFTTLEIDDAGLEKSSAEAILAILRK 182          
BLAST of mRNA_D-dudresnayi_contig9796.28602.1 vs. uniprot
Match: A0A0F6RCA1_9GAMM (Glutathione-dependent peroxiredoxin n=1 Tax=Kangiella geojedonensis TaxID=914150 RepID=A0A0F6RCA1_9GAMM)

HSP 1 Score: 149 bits (375), Expect = 7.510e-42
Identity = 72/131 (54.96%), Postives = 95/131 (72.52%), Query Frame = 2
Query:  125 EILRAHKKAVIFAVPGAFTPTCSEKHLPGFIERAHSMREAGVEEIYCLSVNDLFVMKAWGKSYATFADSGIKLVADGNADYSKAIGMELDLSAHRLGLRCKRFAAIVENGVFTAVEVDDSGLDKSSAESIL 517
            +IL +  K V+FAVPGAFTPTCS  HLPGF+      +E GV+ + C+SVND FVM AW K         + ++ADGNAD+S+A+G+++D +A+ +G+R KRFA I E+GV TA+EVDD GLDKSSAE IL
Sbjct:   27 DILFSGNKVVLFAVPGAFTPTCSAAHLPGFVVNFDQFKEKGVDTVACMSVNDAFVMNAWSKDQNA---ENLLMIADGNADFSEALGLDVDATAYGMGVRSKRFALIAEDGVVTALEVDDKGLDKSSAEHIL 154          
BLAST of mRNA_D-dudresnayi_contig9796.28602.1 vs. uniprot
Match: A0A1Z9BJP2_9BACT (Glutathione-dependent peroxiredoxin n=2 Tax=Bacteria TaxID=2 RepID=A0A1Z9BJP2_9BACT)

HSP 1 Score: 148 bits (374), Expect = 1.060e-41
Identity = 79/135 (58.52%), Postives = 98/135 (72.59%), Query Frame = 2
Query:  125 EILRAHKKAVIFAVPGAFTPTCSEKHLPGFIERAHSMREAGVEEIYCLSVNDLFVMKAWGKSYATFADSGIKLVADGNADYSKAIGMELDLSAHRLGLRCKRFAAIVENGVFTAVEVDDS-GLDKSSAESILAIL 526
            E L   KK V+FAVPGAFTPTCSE+HLPGFIE+A  ++  GV++I CLSVND FVMKAWGK     AD  + ++ADGNAD S+A+G++ D S    G+R +RFAAIVE+GV T + V+     D SSAESILA L
Sbjct:   25 EQLFGGKKVVLFAVPGAFTPTCSEQHLPGFIEKASEIKAKGVDDIICLSVNDAFVMKAWGKDRG--ADGKVTMIADGNADLSRALGLDFDGSGIGFGVRAQRFAAIVEDGVVTKLAVEQPMKFDVSSAESILAAL 157          
BLAST of mRNA_D-dudresnayi_contig9796.28602.1 vs. uniprot
Match: A0A2M7GLW9_9PROT (Glutathione-dependent peroxiredoxin n=6 Tax=Rhodospirillales TaxID=204441 RepID=A0A2M7GLW9_9PROT)

HSP 1 Score: 147 bits (371), Expect = 3.300e-41
Identity = 72/129 (55.81%), Postives = 96/129 (74.42%), Query Frame = 2
Query:  143 KKAVIFAVPGAFTPTCSEKHLPGFIERAHSMREAGVEEIYCLSVNDLFVMKAWGKSYATFADSGIKLVADGNADYSKAIGMELDLSAHRLGLRCKRFAAIVENGVFTAVEVDDSG-LDKSSAESILAIL 526
            KK  IF VPGAFTPTCS KHLPGFI +A  ++  G+++I C+SVND FVM AW K     AD  + LV DG+A+++KA G+ELDL+A  LGLRC+RFA +V++G   +VE+D +G  +K+SAES+LA L
Sbjct:   34 KKVAIFGVPGAFTPTCSAKHLPGFIAKADDLKAKGIDQIVCMSVNDAFVMGAWAKDQG--ADDKVLLVGDGSAEFTKAAGLELDLNARGLGLRCQRFAMVVDDGTVKSVEIDAAGTFEKTSAESVLASL 160          
BLAST of mRNA_D-dudresnayi_contig9796.28602.1 vs. uniprot
Match: C7RCK1_KANKD (Glutathione-dependent peroxiredoxin n=3 Tax=Kangiella TaxID=261963 RepID=C7RCK1_KANKD)

HSP 1 Score: 145 bits (367), Expect = 1.210e-40
Identity = 73/128 (57.03%), Postives = 99/128 (77.34%), Query Frame = 2
Query:  143 KKAVIFAVPGAFTPTCSEKHLPGFIERAHSMREAGVEEIYCLSVNDLFVMKAWGKSYATFADSGIKLVADGNADYSKAIGMELDLSAHRLGLRCKRFAAIVENGVFTAVEVDDSGLDKSSAESILAIL 526
            KK V+FAVPGAFTPTCS  HLPGF+ +A  ++  GV+ I C+SVND+FVM AWGK  A  AD  I ++ADGNAD+++A+G+E+D +   +G+R KRFA IV++GV  A+EVD+ G +KSSAE+IL+ L
Sbjct:   33 KKVVLFAVPGAFTPTCSAAHLPGFVVQADDIKSKGVDTIACMSVNDVFVMDAWGK--AQNADE-IMMLADGNADFTEAMGIEMDATGFGMGVRSKRFAMIVDDGVVKALEVDEKGFEKSSAENILSKL 157          
BLAST of mRNA_D-dudresnayi_contig9796.28602.1 vs. uniprot
Match: A0A850KY37_9GAMM (Glutathione-dependent peroxiredoxin n=1 Tax=Kangiellaceae bacterium TaxID=2026751 RepID=A0A850KY37_9GAMM)

HSP 1 Score: 145 bits (367), Expect = 1.240e-40
Identity = 72/129 (55.81%), Postives = 97/129 (75.19%), Query Frame = 2
Query:  143 KKAVIFAVPGAFTPTCSEKHLPGFIERAHSMREAGVEEIYCLSVNDLFVMKAWGKSYATFADSGIKLVADGNADYSKAIGMELDLSAHRLG-LRCKRFAAIVENGVFTAVEVDDSGLDKSSAESILAIL 526
            KK V+FAVPGAFTPTCS  HLPGF+ +A +++  GV+ I C+SVND+FVM AWGKS        I +VADGNAD+++A+G+++D +A  +G +R KRFA I  +GV  A+EVD+ GLDKSSAE +L +L
Sbjct:   33 KKVVLFAVPGAFTPTCSAAHLPGFVVQADAIKAKGVDSIVCMSVNDVFVMNAWGKSQNA---EEIMMVADGNADFTEALGLDVDATAWGMGAVRSKRFALIANDGVVEAIEVDEKGLDKSSAEHVLTLL 158          
BLAST of mRNA_D-dudresnayi_contig9796.28602.1 vs. uniprot
Match: A0A6L5QQA2_9GAMM (Glutathione-dependent peroxiredoxin n=1 Tax=Kangiella sp. HZ709 TaxID=2666328 RepID=A0A6L5QQA2_9GAMM)

HSP 1 Score: 145 bits (366), Expect = 1.700e-40
Identity = 69/129 (53.49%), Postives = 96/129 (74.42%), Query Frame = 2
Query:  140 HKKAVIFAVPGAFTPTCSEKHLPGFIERAHSMREAGVEEIYCLSVNDLFVMKAWGKSYATFADSGIKLVADGNADYSKAIGMELDLSAHRLGLRCKRFAAIVENGVFTAVEVDDSGLDKSSAESILAIL 526
            +KK ++F+VPGAFTPTCS  HLPGF+  A   +  G++EI C+SVND+FVM AWGKS        I ++ADGN +++KA+G+E+D +A  +GLR KRFA IV++G    + VDD GL+KSSAE++LA+L
Sbjct:   32 NKKVILFSVPGAFTPTCSAAHLPGFVVNADKFKSKGIDEIVCMSVNDVFVMDAWGKSSNA---EEITMLADGNGEFAKALGLEVDGTAWGMGLRSKRFAMIVDDGEVKQIAVDDKGLEKSSAEAVLAML 157          
BLAST of mRNA_D-dudresnayi_contig9796.28602.1 vs. uniprot
Match: A0A1B3B9Z5_9GAMM (Glutathione-dependent peroxiredoxin n=2 Tax=Kangiella TaxID=261963 RepID=A0A1B3B9Z5_9GAMM)

HSP 1 Score: 144 bits (364), Expect = 3.410e-40
Identity = 71/130 (54.62%), Postives = 97/130 (74.62%), Query Frame = 2
Query:  137 AHKKAVIFAVPGAFTPTCSEKHLPGFIERAHSMREAGVEEIYCLSVNDLFVMKAWGKSYATFADSGIKLVADGNADYSKAIGMELDLSAHRLGLRCKRFAAIVENGVFTAVEVDDSGLDKSSAESILAIL 526
            A KK V+FAVPGAFTPTCS  HLPGF+     ++E GV+ + C++VND FVM AWGKS    AD+ I +VADGNAD+S+A+G+E+D +   +G+R KRFA + E+G  T +E+D+ G +KSSAE+IL+ L
Sbjct:   31 ADKKVVMFAVPGAFTPTCSAAHLPGFVVNYDKLKEKGVDTVACMAVNDAFVMDAWGKSQN--ADN-IMMVADGNADFSEALGLEMDATGFGMGVRSKRFALVAEDGKVTELEIDEKGFEKSSAENILSKL 157          
BLAST of mRNA_D-dudresnayi_contig9796.28602.1 vs. uniprot
Match: A0A7X7SSQ4_9GAMM (Glutathione-dependent peroxiredoxin n=1 Tax=Xanthomonadaceae bacterium TaxID=1926873 RepID=A0A7X7SSQ4_9GAMM)

HSP 1 Score: 144 bits (362), Expect = 7.470e-40
Identity = 71/129 (55.04%), Postives = 98/129 (75.97%), Query Frame = 2
Query:  143 KKAVIFAVPGAFTPTCSEKHLPGFIERAHSMREAGVEEIYCLSVNDLFVMKAWGKSYATFADSGIKLVADGNADYSKAIGMELDLSAHRLGLRCKRFAAIVENGVFTAVEVDDSGLDK-SSAESILAIL 526
            KK V+F+VPGAFTPTC  KHLPGF+ERA  +R  GV+ I C++VND FVMKAWGKS     D  ++++ADGNA+Y+KA+G+E+D +   +G+R +RFA IV++GV  +V V++ G  K SSAE++LA L
Sbjct:   34 KKVVLFSVPGAFTPTCDAKHLPGFVERADEIRAKGVDTIACIAVNDPFVMKAWGKSQG--CDGKVQMLADGNAEYTKALGLEMDATGFGMGIRGQRFALIVDDGVVKSVNVEEKGEFKVSSAENVLAQL 160          
BLAST of mRNA_D-dudresnayi_contig9796.28602.1 vs. uniprot
Match: A0A7C7U1S0_9DELT (Glutathione-dependent peroxiredoxin n=1 Tax=Myxococcales bacterium TaxID=2026763 RepID=A0A7C7U1S0_9DELT)

HSP 1 Score: 143 bits (361), Expect = 9.650e-40
Identity = 71/129 (55.04%), Postives = 96/129 (74.42%), Query Frame = 2
Query:  143 KKAVIFAVPGAFTPTCSEKHLPGFIERAHSMREAGVEEIYCLSVNDLFVMKAWGKSYATFADSGIKLVADGNADYSKAIGMELDLSAHRLGLRCKRFAAIVENGVFTAVEVDDSG-LDKSSAESILAIL 526
            KK V+FAVPGAFTPTCS +HLPGF+E+A ++R  GV+++ C+SVND FVM AWG+     A   ++++ADGN D SKA+G++ D S    GLRC+RFAAIVE+GV T + V+  G  + SSAE+IL+ L
Sbjct:   31 KKVVLFAVPGAFTPTCSNQHLPGFVEQADAIRAKGVDDVICMSVNDAFVMGAWGEDRG--ASDRVRMIADGNGDLSKALGLDFDGSGIGFGLRCQRFAAIVEDGVVTTLAVEAPGKFEVSSAEAILSAL 157          
The following BLAST results are available for this feature:
BLAST of mRNA_D-dudresnayi_contig9796.28602.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S3H5S6_9STRA2.240e-9883.72Hypothetical protein n=1 Tax=Spumella elongata Tax... [more]
A0A0F6RCA1_9GAMM7.510e-4254.96Glutathione-dependent peroxiredoxin n=1 Tax=Kangie... [more]
A0A1Z9BJP2_9BACT1.060e-4158.52Glutathione-dependent peroxiredoxin n=2 Tax=Bacter... [more]
A0A2M7GLW9_9PROT3.300e-4155.81Glutathione-dependent peroxiredoxin n=6 Tax=Rhodos... [more]
C7RCK1_KANKD1.210e-4057.03Glutathione-dependent peroxiredoxin n=3 Tax=Kangie... [more]
A0A850KY37_9GAMM1.240e-4055.81Glutathione-dependent peroxiredoxin n=1 Tax=Kangie... [more]
A0A6L5QQA2_9GAMM1.700e-4053.49Glutathione-dependent peroxiredoxin n=1 Tax=Kangie... [more]
A0A1B3B9Z5_9GAMM3.410e-4054.62Glutathione-dependent peroxiredoxin n=2 Tax=Kangie... [more]
A0A7X7SSQ4_9GAMM7.470e-4055.04Glutathione-dependent peroxiredoxin n=1 Tax=Xantho... [more]
A0A7C7U1S0_9DELT9.650e-4055.04Glutathione-dependent peroxiredoxin n=1 Tax=Myxoco... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
D-dudresnayi_contig9796contigD-dudresnayi_contig9796:4248..4960 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Desmarestia dudresnayi DdudBR16 monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score146.4
Seed ortholog evalue1.3e-32
Seed eggNOG ortholog1122134.KB893650_gene1320
Preferred nameMA20_20905
KEGG koko:K03386
KEGG Pathwayko04214,map04214
EggNOG free text desc.Peroxiredoxin
EggNOG OGs1MU0H@1224,1RRFB@1236,1XK41@135619,COG0678@1,COG0678@2
EC1.11.1.15
COG Functional cat.O
Best tax levelOceanospirillales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko04147
Hectar predicted targeting categoryother localisation
Exons1
Model size713
Cds size516
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_D-dudresnayi_contig9796.28602.1prot_D-dudresnayi_contig9796.28602.1Desmarestia dudresnayi DdudBR16 monoicouspolypeptideD-dudresnayi_contig9796 4267..4782 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930936.634818-UTR-D-dudresnayi_contig9796:4247..42661622930936.634818-UTR-D-dudresnayi_contig9796:4247..4266Desmarestia dudresnayi DdudBR16 monoicousUTRD-dudresnayi_contig9796 4248..4266 +
1690557971.9468703-UTR-D-dudresnayi_contig9796:4247..42661690557971.9468703-UTR-D-dudresnayi_contig9796:4247..4266Desmarestia dudresnayi DdudBR16 monoicousUTRD-dudresnayi_contig9796 4248..4266 +
1622930936.6661868-UTR-D-dudresnayi_contig9796:4782..49601622930936.6661868-UTR-D-dudresnayi_contig9796:4782..4960Desmarestia dudresnayi DdudBR16 monoicousUTRD-dudresnayi_contig9796 4783..4960 +
1690557971.9800034-UTR-D-dudresnayi_contig9796:4782..49601690557971.9800034-UTR-D-dudresnayi_contig9796:4782..4960Desmarestia dudresnayi DdudBR16 monoicousUTRD-dudresnayi_contig9796 4783..4960 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930936.6501627-CDS-D-dudresnayi_contig9796:4266..47821622930936.6501627-CDS-D-dudresnayi_contig9796:4266..4782Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig9796 4267..4782 +
1690557971.9634597-CDS-D-dudresnayi_contig9796:4266..47821690557971.9634597-CDS-D-dudresnayi_contig9796:4266..4782Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig9796 4267..4782 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_D-dudresnayi_contig9796.28602.1

>prot_D-dudresnayi_contig9796.28602.1 ID=prot_D-dudresnayi_contig9796.28602.1|Name=mRNA_D-dudresnayi_contig9796.28602.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=polypeptide|length=172bp
MSTSIAANSPIPDQIVDIVEPVSGDYVIKAGQNFGEILRAHKKAVIFAVP
GAFTPTCSEKHLPGFIERAHSMREAGVEEIYCLSVNDLFVMKAWGKSYAT
FADSGIKLVADGNADYSKAIGMELDLSAHRLGLRCKRFAAIVENGVFTAV
EVDDSGLDKSSAESILAILRK*
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mRNA from alignment at D-dudresnayi_contig9796:4248..4960+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_D-dudresnayi_contig9796.28602.1 ID=mRNA_D-dudresnayi_contig9796.28602.1|Name=mRNA_D-dudresnayi_contig9796.28602.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=mRNA|length=713bp|location=Sequence derived from alignment at D-dudresnayi_contig9796:4248..4960+ (Desmarestia dudresnayi DdudBR16 monoicous)
GTTAACTTTGTCATCAAAAATGAGTACCTCTATTGCAGCAAACAGTCCTA TCCCTGATCAGATTGTTGATATTGTGGAGCCAGTTAGTGGTGACTATGTG ATCAAGGCTGGCCAGAACTTTGGCGAGATCTTGCGTGCCCACAAGAAGGC TGTTATCTTCGCTGTTCCTGGCGCTTTTACCCCCACTTGCAGTGAAAAGC ATCTTCCTGGATTCATCGAGAGAGCTCATTCTATGCGCGAGGCTGGTGTT GAGGAGATTTACTGCCTTTCCGTGAACGACTTGTTTGTAATGAAGGCATG GGGTAAGTCTTATGCTACCTTTGCTGACAGTGGCATCAAGCTTGTCGCCG ATGGAAACGCAGATTATTCTAAGGCCATTGGTATGGAGCTGGATCTTTCT GCTCATCGCCTGGGTCTCAGATGCAAGCGCTTTGCTGCCATTGTCGAGAA CGGTGTTTTCACTGCCGTTGAAGTTGACGATTCCGGTCTGGACAAATCCT CCGCTGAGTCCATCCTTGCCATCCTCAGAAAGTAAGGTGGAAGTGAGACA ATCCTTGGTTGATATATCTTTCCGTAATACTAAAGGTTGATGTTTCATGT AACTTTTTCATCTGATTAGCTTACTGTACTGCTGTTCAATACAACAATAC AGTAGTGAGCTGATGACTGATTGGTATATTGCTCCTGAATCATTGTAATA AAAAATAACTTCG
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Coding sequence (CDS) from alignment at D-dudresnayi_contig9796:4248..4960+

>mRNA_D-dudresnayi_contig9796.28602.1 ID=mRNA_D-dudresnayi_contig9796.28602.1|Name=mRNA_D-dudresnayi_contig9796.28602.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=CDS|length=1032bp|location=Sequence derived from alignment at D-dudresnayi_contig9796:4248..4960+ (Desmarestia dudresnayi DdudBR16 monoicous)
ATGAGTACCTCTATTGCAGCAAACAGTCCTATCCCTGATCAGATTGTTGA
TATTGTGGAGCCAGTTAGTGGTGACTATGTGATCAAGGCTGGCCAGAACT
TTGGCGAGATCTTGCGTGCCCACAAGAAGGCTGTTATCTTCGCTGTTCCT
GGCGCTTTTACCCCCACTTGCAGTGAAAAGCATCTTCCTGGATTCATCGA
GAGAGCTCATTCTATGCGCGAGGCTGGTGTTGAGGAGATTTACTGCCTTT
CCGTGAACGACTTGTTTGTAATGAAGGCATGGGGTAAGTCTTATGCTACC
TTTGCTGACAGTGGCATCAAGCTTGTCGCCGATGGAAACGCAGATTATTC
TAAGGCCATTGGTATGGAGCTGGATCTTTCTGCTCATCGCCTGGGTCTCA
GATGCAAGCGCTTTGCTGCCATTGTCGAGAACGGTGTTTTCACTGCCGTT
GAAGTTGACGATTCCGGTCTGGACAAATCCTCCGCTGAGTCCATCCTTGC
CATCCTCAGAAAGTAAATGAGTACCTCTATTGCAGCAAACAGTCCTATCC
CTGATCAGATTGTTGATATTGTGGAGCCAGTTAGTGGTGACTATGTGATC
AAGGCTGGCCAGAACTTTGGCGAGATCTTGCGTGCCCACAAGAAGGCTGT
TATCTTCGCTGTTCCTGGCGCTTTTACCCCCACTTGCAGTGAAAAGCATC
TTCCTGGATTCATCGAGAGAGCTCATTCTATGCGCGAGGCTGGTGTTGAG
GAGATTTACTGCCTTTCCGTGAACGACTTGTTTGTAATGAAGGCATGGGG
TAAGTCTTATGCTACCTTTGCTGACAGTGGCATCAAGCTTGTCGCCGATG
GAAACGCAGATTATTCTAAGGCCATTGGTATGGAGCTGGATCTTTCTGCT
CATCGCCTGGGTCTCAGATGCAAGCGCTTTGCTGCCATTGTCGAGAACGG
TGTTTTCACTGCCGTTGAAGTTGACGATTCCGGTCTGGACAAATCCTCCG
CTGAGTCCATCCTTGCCATCCTCAGAAAGTAA
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